Moc04g36440 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g36440
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionCalcium-binding EF-hand family protein
Locationchr4: 27351723 .. 27352277 (+)
RNA-Seq ExpressionMoc04g36440
SyntenyMoc04g36440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCTGAATCTGTACAACCTTTCAATGGCGGAGAAAACCGATCGCCCCGAAAATCCCTCTCTCCGCTTCCTTATAATGATCGATTGCCTCGTGTTTCAGAAACTAACCTCGTGGCTCAGCGCCACATACCACGGCTTCGCCTCCACAAGAAATTCAAATCGTTGTTCCCCCATTCCGAAAGCGGTCGTAGACGGCGGCGAGAGTGTGAGCAGAGAGGAGATGAAATCGGTGATGGGAGATTTGGGGATTGTTTGCGGCGAGGAGGGCGATGAATCGCTTCCTCTGTTCGGATCTGTTGAGGAAATCGCGAAGCTGTTCGAGGAGAGAGGGCCGTGTATTGCGGAGGTGAAGAGAGCTTTCGACGTTTTCGACGCCAATGGCGATGGATTCATCGATGTGGTGGAGCTTCAGAGAGTTCTCTGCGTTTTGGGCCTCAAAGAAGGGGAGGGGGTTGAGAATTGCAAGAGGATGATCCAGAAGTTTGATGAAAATGAAGATGGCAAAATTGATTTTGAAGAGTTTGTTAAGTTGATGGAGTCTGGTTTCAATTGA

mRNA sequence

ATGGATCTGAATCTGTACAACCTTTCAATGGCGGAGAAAACCGATCGCCCCGAAAATCCCTCTCTCCGCTTCCTTATAATGATCGATTGCCTCGTGTTTCAGAAACTAACCTCGTGGCTCAGCGCCACATACCACGGCTTCGCCTCCACAAGAAATTCAAATCGTTGTTCCCCCATTCCGAAAGCGGTCGTAGACGGCGGCGAGAGTGTGAGCAGAGAGGAGATGAAATCGGTGATGGGAGATTTGGGGATTGTTTGCGGCGAGGAGGGCGATGAATCGCTTCCTCTGTTCGGATCTGTTGAGGAAATCGCGAAGCTGTTCGAGGAGAGAGGGCCGTGTATTGCGGAGGTGAAGAGAGCTTTCGACGTTTTCGACGCCAATGGCGATGGATTCATCGATGTGGTGGAGCTTCAGAGAGTTCTCTGCGTTTTGGGCCTCAAAGAAGGGGAGGGGGTTGAGAATTGCAAGAGGATGATCCAGAAGTTTGATGAAAATGAAGATGGCAAAATTGATTTTGAAGAGTTTGTTAAGTTGATGGAGTCTGGTTTCAATTGA

Coding sequence (CDS)

ATGGATCTGAATCTGTACAACCTTTCAATGGCGGAGAAAACCGATCGCCCCGAAAATCCCTCTCTCCGCTTCCTTATAATGATCGATTGCCTCGTGTTTCAGAAACTAACCTCGTGGCTCAGCGCCACATACCACGGCTTCGCCTCCACAAGAAATTCAAATCGTTGTTCCCCCATTCCGAAAGCGGTCGTAGACGGCGGCGAGAGTGTGAGCAGAGAGGAGATGAAATCGGTGATGGGAGATTTGGGGATTGTTTGCGGCGAGGAGGGCGATGAATCGCTTCCTCTGTTCGGATCTGTTGAGGAAATCGCGAAGCTGTTCGAGGAGAGAGGGCCGTGTATTGCGGAGGTGAAGAGAGCTTTCGACGTTTTCGACGCCAATGGCGATGGATTCATCGATGTGGTGGAGCTTCAGAGAGTTCTCTGCGTTTTGGGCCTCAAAGAAGGGGAGGGGGTTGAGAATTGCAAGAGGATGATCCAGAAGTTTGATGAAAATGAAGATGGCAAAATTGATTTTGAAGAGTTTGTTAAGTTGATGGAGTCTGGTTTCAATTGA

Protein sequence

MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMESGFN
Homology
[Close]
BLAST of Moc04g36440 vs. NCBI nr
Match: XP_022133625.1 (probable calcium-binding protein CML45 [Momordica charantia])

HSP 1 Score: 373.2 bits (957), Expect = 1.3e-99
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0 

Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP
Sbjct: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60

Query: 61  KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA 120
           KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA
Sbjct: 61  KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA 120

Query: 121 FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME 180
           FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME
Sbjct: 121 FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME 180

Query: 181 SGFN 185
           SGFN
Sbjct: 181 SGFN 184

[Close]
BLAST of Moc04g36440 vs. NCBI nr
Match: XP_038889669.1 (probable calcium-binding protein CML46 [Benincasa hispida])

HSP 1 Score: 203.4 bits (516), Expect = 1.7e-48
Identity = 110/184 (59.78%), Postives = 134/184 (72.83%), Query Frame = 0 

Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSP-I 60
           MD  +YN+SM +KT   +NPS RFLI+ID L FQKL SWL    + FA+T+      P  
Sbjct: 1   MDFGVYNVSMDDKT-TTQNPSFRFLIVIDFLRFQKLASWL----YAFATTKQKPSSFPAT 60

Query: 61  PKAVVDGGES--VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEV 120
            K    GGE   ++ EEMK VMG+LGI CGE   ES+PLFGS EE+A++F+ER     EV
Sbjct: 61  SKTEEHGGEERLMTTEEMKWVMGNLGIDCGE---ESVPLFGSTEEVAEMFDERAASTVEV 120

Query: 121 KRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVK 180
           K+AFDVFD NGDGFID  ELQRV+CVLG KEGE ++NC+RMI+KFD N DG+IDFEEF+K
Sbjct: 121 KKAFDVFDVNGDGFIDSEELQRVMCVLGFKEGERIQNCERMIRKFDHNGDGRIDFEEFLK 176

Query: 181 LMES 182
           LMES
Sbjct: 181 LMES 176

[Close]
BLAST of Moc04g36440 vs. NCBI nr
Match: XP_022993675.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 199.5 bits (506), Expect = 2.5e-47
Identity = 104/170 (61.18%), Postives = 121/170 (71.18%), Query Frame = 0 

Query: 17  PENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAV-----VDGGESVS 76
           P NPS RFLIMIDC  FQKL SWL    + FAS +      P  +        +GGE VS
Sbjct: 7   PANPSFRFLIMIDCFRFQKLGSWL----YAFASGKRKQWSLPASETAKSEQRKEGGERVS 66

Query: 77  REEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGF 136
           REEMK VM +LG+  GEE +ESLP F S+EE+ K+F++     AEVK AFDVFD N DGF
Sbjct: 67  REEMKWVMEELGLSWGEETEESLPGFASMEEMCKIFDQTAASAAEVKEAFDVFDVNSDGF 126

Query: 137 IDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMES 182
           IDV ELQRVLCVLG KEGE ++NC+RMI KFDEN DG+IDF+EFVKLMES
Sbjct: 127 IDVEELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMES 172

[Close]
BLAST of Moc04g36440 vs. NCBI nr
Match: XP_031741770.1 (probable calcium-binding protein CML47 [Cucumis sativus])

HSP 1 Score: 198.4 bits (503), Expect = 5.5e-47
Identity = 107/184 (58.15%), Postives = 135/184 (73.37%), Query Frame = 0 

Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPI- 60
           M+L LYN+SM +K+  P+NPS RFL++ID L FQ L SWL ++     ST+      P  
Sbjct: 132 MNLGLYNVSMDDKS-TPQNPSFRFLLIIDFLRFQNLASWLCSS----TSTKLKQSTPPAS 191

Query: 61  PKAVVDGGES--VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEV 120
            K+V    E   VS EEMK VMG+LGI C E    S+PLFGS EE+A++F++    +AEV
Sbjct: 192 SKSVESRAEERLVSTEEMKWVMGNLGIDCSE--GSSVPLFGSAEEVAEMFDQTAASVAEV 251

Query: 121 KRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVK 180
           K+AF VFD NGDGFIDV ELQRV+CVLG KEGEG+ENC++MI+KFD N+DG+IDFEEFVK
Sbjct: 252 KQAFGVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVK 308

Query: 181 LMES 182
           LME+
Sbjct: 312 LMEA 308

[Close]
BLAST of Moc04g36440 vs. NCBI nr
Match: XP_008459700.2 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 197.2 bits (500), Expect = 1.2e-46
Identity = 108/186 (58.06%), Postives = 133/186 (71.51%), Query Frame = 0 

Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           M+  LYN+SM +K+  P+NPS RFLI+ID L FQ L SWL +    FAST+      P  
Sbjct: 1   MNFGLYNVSMDDKS-TPQNPSFRFLIIIDFLRFQNLASWLCS----FASTKLKQSTLPAS 60

Query: 61  KAVVD--GGES---VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIA 120
              V+   GE    VS EEMK VMG+LG+ C E    S+PLFGS EE+A++F++     A
Sbjct: 61  SKSVESSAGERERLVSTEEMKWVMGNLGMDCSE--GSSVPLFGSAEEVAEMFDQTAASAA 120

Query: 121 EVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEF 180
           EVK+AFDVFD N DGFIDV ELQRV+CVLG KEGEG+ENC++MI KFD N+DG+IDFEEF
Sbjct: 121 EVKQAFDVFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEF 179

Query: 181 VKLMES 182
           VKLME+
Sbjct: 181 VKLMEA 179

[Close]
BLAST of Moc04g36440 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 6.6e-27
Identity = 61/119 (51.26%), Postives = 85/119 (71.43%), Query Frame = 0 

Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVF 124
           D  + + RE++  VM  LG+   +E +E L    S +E++ LFEE+ P + EVK+AFDVF
Sbjct: 86  DDDDGLCREDVGMVMKSLGLSTDQE-NEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVF 145

Query: 125 DANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMESGF 184
           D N DGFID ++LQRVL +LGLK+G  +ENC+RMI+ FD ++DG+IDF  FVK ME+ F
Sbjct: 146 DENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFMENNF 203

[Close]
BLAST of Moc04g36440 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.1e-25
Identity = 61/119 (51.26%), Postives = 79/119 (66.39%), Query Frame = 0 

Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVF 124
           D    +SREE + VM  LG+      D+ L    S +E++ LFEE+   + EVK+AFDVF
Sbjct: 78  DDDIDISREEAEMVMRSLGLF---YNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVF 137

Query: 125 DANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMESGF 184
           D N DGFID +ELQRVL +LG K+G  ++NC  MI+  D N+DGKIDF EFVK ME+ F
Sbjct: 138 DENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETSF 193

[Close]
BLAST of Moc04g36440 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.7e-14
Identity = 51/144 (35.42%), Postives = 73/144 (50.69%), Query Frame = 0 

Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFG----------SVEEIAKLF------- 124
           +G   ++REE++  +G LGI      DE   +             VEE  +L+       
Sbjct: 103 NGDGRITREELEDSLGKLGIPV--PADELAAVIARIDANGDGCVDVEEFGELYRSIMAGG 162

Query: 125 ---------EERGPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRM 183
                    EE      +++ AF VFDANGDG+I V EL  VL  LGLK+G   E C+RM
Sbjct: 163 DDSKDGRAKEEEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLKQGRTAEECRRM 222

[Close]
BLAST of Moc04g36440 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.2e-14
Identity = 61/154 (39.61%), Postives = 87/154 (56.49%), Query Frame = 0 

Query: 39  WLSATYHGFASTRNSNRCSPIP---KAVVDGGESV--SREEMKSVMGD---LGIVCGEEG 98
           + S  Y  F S RN+ + + +P   K VV     +  S EE+K+++ D   L     EEG
Sbjct: 42  FFSNAYILFTSIRNNKQNTELPIIKKVVVPNRADIKTSVEEVKAIIDDSEALYECLIEEG 101

Query: 99  DESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGE 158
           +E L     +E+   + +E       VK AF +FD N DGFID  EL+ VL +LG  E  
Sbjct: 102 EEYL-----LEKNEMMGKE------IVKEAFRLFDENQDGFIDENELKHVLSLLGYDECT 161

Query: 159 GVENCKRMIQKFDENEDGKIDFEEFVKLMESGFN 185
            +E C++M++ +DEN DGKIDF EFVKL+E  F+
Sbjct: 162 KME-CRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

[Close]
BLAST of Moc04g36440 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.8e-14
Identity = 47/129 (36.43%), Postives = 76/129 (58.91%), Query Frame = 0 

Query: 65  DGGESVSREEMKSVMGDLGIVCGEEG----DESLPLFG----SVEEIAKLFE---ERGPC 124
           +G   ++++E+   + +LGI   ++      E + L G     +EE   L++   E    
Sbjct: 16  NGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDE 75

Query: 125 IAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFE 183
             +++ AF+VFD N DGFI V EL+ VL  LGLK+G  +E+CKRMI K D + DG ++F+
Sbjct: 76  EEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFK 135

[Close]
BLAST of Moc04g36440 vs. ExPASy TrEMBL
Match: A0A6J1BVS4 (probable calcium-binding protein CML45 OS=Momordica charantia OX=3673 GN=LOC111006164 PE=4 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 6.1e-100
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0 

Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP
Sbjct: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60

Query: 61  KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA 120
           KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA
Sbjct: 61  KAVVDGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRA 120

Query: 121 FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME 180
           FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME
Sbjct: 121 FDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLME 180

Query: 181 SGFN 185
           SGFN
Sbjct: 181 SGFN 184

[Close]
BLAST of Moc04g36440 vs. ExPASy TrEMBL
Match: A0A6J1JZ70 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111489598 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.2e-47
Identity = 104/170 (61.18%), Postives = 121/170 (71.18%), Query Frame = 0 

Query: 17  PENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAV-----VDGGESVS 76
           P NPS RFLIMIDC  FQKL SWL    + FAS +      P  +        +GGE VS
Sbjct: 7   PANPSFRFLIMIDCFRFQKLGSWL----YAFASGKRKQWSLPASETAKSEQRKEGGERVS 66

Query: 77  REEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGF 136
           REEMK VM +LG+  GEE +ESLP F S+EE+ K+F++     AEVK AFDVFD N DGF
Sbjct: 67  REEMKWVMEELGLSWGEETEESLPGFASMEEMCKIFDQTAASAAEVKEAFDVFDVNSDGF 126

Query: 137 IDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMES 182
           IDV ELQRVLCVLG KEGE ++NC+RMI KFDEN DG+IDF+EFVKLMES
Sbjct: 127 IDVEELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMES 172

[Close]
BLAST of Moc04g36440 vs. ExPASy TrEMBL
Match: A0A0A0KS94 (Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 2.7e-47
Identity = 107/184 (58.15%), Postives = 135/184 (73.37%), Query Frame = 0 

Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPI- 60
           M+L LYN+SM +K+  P+NPS RFL++ID L FQ L SWL ++     ST+      P  
Sbjct: 2   MNLGLYNVSMDDKS-TPQNPSFRFLLIIDFLRFQNLASWLCSS----TSTKLKQSTPPAS 61

Query: 61  PKAVVDGGES--VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEV 120
            K+V    E   VS EEMK VMG+LGI C E    S+PLFGS EE+A++F++    +AEV
Sbjct: 62  SKSVESRAEERLVSTEEMKWVMGNLGIDCSE--GSSVPLFGSAEEVAEMFDQTAASVAEV 121

Query: 121 KRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVK 180
           K+AF VFD NGDGFIDV ELQRV+CVLG KEGEG+ENC++MI+KFD N+DG+IDFEEFVK
Sbjct: 122 KQAFGVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVK 178

Query: 181 LMES 182
           LME+
Sbjct: 182 LMEA 178

[Close]
BLAST of Moc04g36440 vs. ExPASy TrEMBL
Match: A0A1S3CAB3 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 6.0e-47
Identity = 108/186 (58.06%), Postives = 133/186 (71.51%), Query Frame = 0 

Query: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIP 60
           M+  LYN+SM +K+  P+NPS RFLI+ID L FQ L SWL +    FAST+      P  
Sbjct: 1   MNFGLYNVSMDDKS-TPQNPSFRFLIIIDFLRFQNLASWLCS----FASTKLKQSTLPAS 60

Query: 61  KAVVD--GGES---VSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIA 120
              V+   GE    VS EEMK VMG+LG+ C E    S+PLFGS EE+A++F++     A
Sbjct: 61  SKSVESSAGERERLVSTEEMKWVMGNLGMDCSE--GSSVPLFGSAEEVAEMFDQTAASAA 120

Query: 121 EVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEF 180
           EVK+AFDVFD N DGFIDV ELQRV+CVLG KEGEG+ENC++MI KFD N+DG+IDFEEF
Sbjct: 121 EVKQAFDVFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEF 179

Query: 181 VKLMES 182
           VKLME+
Sbjct: 181 VKLMEA 179

[Close]
BLAST of Moc04g36440 vs. ExPASy TrEMBL
Match: A0A6J1FKT4 (probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC111445127 PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 4.3e-45
Identity = 100/170 (58.82%), Postives = 119/170 (70.00%), Query Frame = 0 

Query: 17  PENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCSPIPKAVV-----DGGESVS 76
           P  PS RFLIMIDC  FQKL SWL    + FA+ +      P  +        +GGE VS
Sbjct: 7   PAIPSFRFLIMIDCFRFQKLGSWL----YAFATGKRKQWSLPASETAKSEQRDEGGERVS 66

Query: 77  REEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGF 136
           REEMK VMG+LG+   EE +ESLP F S+EE+ ++F++     AEVK AFDVFD N DGF
Sbjct: 67  REEMKWVMGELGLSSEEETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDVNSDGF 126

Query: 137 IDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMES 182
           ID  ELQRVLCVLG KEGE ++NC+RMI KFDEN DG+IDF+EFVKLMES
Sbjct: 127 IDGDELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMES 172

[Close]
BLAST of Moc04g36440 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 122.1 bits (305), Expect = 4.7e-28
Identity = 61/119 (51.26%), Postives = 85/119 (71.43%), Query Frame = 0 

Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVF 124
           D  + + RE++  VM  LG+   +E +E L    S +E++ LFEE+ P + EVK+AFDVF
Sbjct: 86  DDDDGLCREDVGMVMKSLGLSTDQE-NEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVF 145

Query: 125 DANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMESGF 184
           D N DGFID ++LQRVL +LGLK+G  +ENC+RMI+ FD ++DG+IDF  FVK ME+ F
Sbjct: 146 DENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFMENNF 203

[Close]
BLAST of Moc04g36440 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 117.1 bits (292), Expect = 1.5e-26
Identity = 61/119 (51.26%), Postives = 79/119 (66.39%), Query Frame = 0 

Query: 65  DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVF 124
           D    +SREE + VM  LG+      D+ L    S +E++ LFEE+   + EVK+AFDVF
Sbjct: 78  DDDIDISREEAEMVMRSLGLF---YNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVF 137

Query: 125 DANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFEEFVKLMESGF 184
           D N DGFID +ELQRVL +LG K+G  ++NC  MI+  D N+DGKIDF EFVK ME+ F
Sbjct: 138 DENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETSF 193

[Close]
BLAST of Moc04g36440 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.6e-15
Identity = 61/154 (39.61%), Postives = 87/154 (56.49%), Query Frame = 0 

Query: 39  WLSATYHGFASTRNSNRCSPIP---KAVVDGGESV--SREEMKSVMGD---LGIVCGEEG 98
           + S  Y  F S RN+ + + +P   K VV     +  S EE+K+++ D   L     EEG
Sbjct: 42  FFSNAYILFTSIRNNKQNTELPIIKKVVVPNRADIKTSVEEVKAIIDDSEALYECLIEEG 101

Query: 99  DESLPLFGSVEEIAKLFEERGPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGE 158
           +E L     +E+   + +E       VK AF +FD N DGFID  EL+ VL +LG  E  
Sbjct: 102 EEYL-----LEKNEMMGKE------IVKEAFRLFDENQDGFIDENELKHVLSLLGYDECT 161

Query: 159 GVENCKRMIQKFDENEDGKIDFEEFVKLMESGFN 185
            +E C++M++ +DEN DGKIDF EFVKL+E  F+
Sbjct: 162 KME-CRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

[Close]
BLAST of Moc04g36440 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 79.7 bits (195), Expect = 2.7e-15
Identity = 47/129 (36.43%), Postives = 76/129 (58.91%), Query Frame = 0 

Query: 65  DGGESVSREEMKSVMGDLGIVCGEEG----DESLPLFG----SVEEIAKLFE---ERGPC 124
           +G   ++++E+   + +LGI   ++      E + L G     +EE   L++   E    
Sbjct: 16  NGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDE 75

Query: 125 IAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFE 183
             +++ AF+VFD N DGFI V EL+ VL  LGLK+G  +E+CKRMI K D + DG ++F+
Sbjct: 76  EEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFK 135

[Close]
BLAST of Moc04g36440 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 79.3 bits (194), Expect = 3.5e-15
Identity = 45/132 (34.09%), Postives = 76/132 (57.58%), Query Frame = 0 

Query: 65  DGGESVSREEMKSVMGDLGIVCGEE------------GDESLPL--FGSVEEIAKLFEER 124
           DG   ++ +E+     +LGI+  E+            GD  + +  FG + +   + +E 
Sbjct: 16  DGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFGELYKTIMVEDED 75

Query: 125 GPCIAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKI 183
                ++K AF+VFD NGDGFI V EL+ VL  LGLK+G+ +E C++MI + D + DG++
Sbjct: 76  EVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRV 135

The following BLAST results are available for this feature:
BLAST of Moc04g36440 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022133625.11.3e-99100.00probable calcium-binding protein CML45 [Momordica charantia][more]
XP_038889669.11.7e-4859.78probable calcium-binding protein CML46 [Benincasa hispida][more]
XP_022993675.12.5e-4761.18probable calcium-binding protein CML30 [Cucurbita maxima][more]
XP_031741770.15.5e-4758.15probable calcium-binding protein CML47 [Cucumis sativus][more]
XP_008459700.21.2e-4658.06PREDICTED: probable calcium-binding protein CML30 [Cucumis melo][more]
BLAST of Moc04g36440 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q93Z276.6e-2751.26Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ... [more]
Q9MBG52.1e-2551.26Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ... [more]
Q0JC441.7e-1435.42Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9SN892.2e-1439.61Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ... [more]
Q9SRR73.8e-1436.43Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1[more]
BLAST of Moc04g36440 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1BVS46.1e-100100.00probable calcium-binding protein CML45 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1JZ701.2e-4761.18probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114895... [more]
A0A0A0KS942.7e-4758.15Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1[more]
A0A1S3CAB36.0e-4758.06probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 P... [more]
A0A6J1FKT44.3e-4558.82probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
BLAST of Moc04g36440 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G39670.14.7e-2851.26Calcium-binding EF-hand family protein [more]
AT3G29000.11.5e-2651.26Calcium-binding EF-hand family protein [more]
AT3G47480.11.6e-1539.61Calcium-binding EF-hand family protein [more]
AT3G07490.12.7e-1536.43ARF-GAP domain 11 [more]
AT4G03290.13.5e-1534.09EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 116..144
e-value: 0.0027
score: 26.9
coord: 154..182
e-value: 1.7E-4
score: 31.0
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 116..180
e-value: 1.7E-12
score: 47.6
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 150..184
score: 13.202322
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 112..147
score: 14.262346
IPR002048EF-hand domainCDDcd00051EFhcoord: 116..179
e-value: 2.21093E-17
score: 70.6545
NoneNo IPR availableGENE3D1.10.238.10coord: 34..184
e-value: 5.0E-21
score: 77.2
NoneNo IPR availablePANTHERPTHR10891:SF805CALCIUM-BINDING PROTEIN CML45-RELATEDcoord: 58..183
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 58..183
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 125..137
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 163..175
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 64..180

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g36440.1Moc04g36440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding