Moc03g28140.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g28140.1
TypemRNA
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptioncysteine-rich receptor-like protein kinase 10
Locationchr3: 20217053 .. 20219650 (-)
Sequence length1866
RNA-Seq ExpressionMoc03g28140.1
SyntenyMoc03g28140.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCAACAAAGAGCGTGGTCTTGGGCATACTCAATTTTCTTAGTTTCATCTTCCTCCCTAGTTTCATCACCGCTCAGACTCTATTACAATACCCATATCGCAGTTGCTTACCCGGCAATTTCACCCAGAACAGCGCCTATCAGTCCAGCCTCAATCTTCTGTTTTTAGAATTCAACAACGTCGCCCACCAAAAAACGTTCCACAGTACGTCCGTTGGTCTGACATCAACCGACACCGTTTACGGTCTCTTCCAGTGCTGGGAAGACGTCACCGACAACTGTGGAGCTTGCGTGGCCACAGCCATCAACGACACGGCCAACAACTTCTGCCGCTTCAGGAAAGGCGCTGTGAGTTGGTACGACGGCTGCATATTGCGCTATTCCAACCAATCTATCTTTTCCGACCTCTCCACCGTCCCAAATCTCATGCTACTCAACACCGCAGATATAGACGCCGACAAGCCTCGCTTCAACCAACTGGTCATGTCCACGTTTGGGGGCACCGTGGCCCGGGCGGCTGACGCCTCTTCCGTGGCCGAAATGGTTGCAACTCGAGAGGCAAATTTCACATTTCATACACTTTACACATTGGCACAGTGCACCGGCGATTTATCCATTTCCAATTGCCGAGAATGCTTACGACAGGCCATCGTTGCTGTCCCGAGTTGTTGCGCAAATAAACGAGGCGGCAGAGTTTTGTATCCCAGCTGCTACGTTCGTTACGAAATTTACCCCTTTTACCGACTCACCGCCAACAATCCTCCACCAATTCCCCCGTCCACTCCCAAAACTCCACCACTGCCTCCTCCTCCTCATGCTCCCTCAGGTAATTGATATGACTTAAATTATATTAGTATAATTGTGCTGTGCTATCCAATTTTATTGGGTAATTTGTAAAAACAGGGGAAACCAGATCCTCCACTCTCATAATTCTGGTCACTGTGCTTCCAATTACCGTCTCCATTCTGCTTTTCGTCGGCTGGTGTTTCCTACGCCAGAGAGCAAAGAAGAGATACACTACTGTAAAGGAAGATCACAGTGGTATACGTTATTCTTTCCACACTAGCTCTGAAATTCAAGTTGGTTGATTTCTGAAAAATGAGATGCCAAGTTTGGACATAATTTGTGGGGTATGGCTTAATTTGTGCAGTTGTTAGTGGGATTAGCACTGTGGAGTCTCTGCAATTTGATTTCAATACAATCGATGCCGCTACTAACAAATTCTCAGCAGAGAACAGGATAGGTGAAGGTGGATTTGGTGCAGTCTACAAGGTACCCAAACAAATCCTTCACTTCTAATCTTTGTGTAATTAGCAAATTTCATTTGCTACTGATTTAGAAGCACGTTATTTGTTTCAGGGAAGGCTTGAAAATGGGCAAGAGATAGCAGTGAAAAGACTATCGAGAGGATCTTTGCAAGGCTCTGAAGAGTTCAAGAATGAGGTTATGTTGGTTGCTAAGCTTCAACATAGAAATCTAGTGAGGCTTTTGGGATTCTGCTTGGAGGGTGAAGAAAAGATTCTCATCTATGAATATATTCCCAACAAAAGCCTTGATTACTTCCTATTTGGTTTGTTCATTTCACACATTACCTTTCTTTCTACCTTTATTCATTTACTTTATACTTTAGAGTTTGTTTGATTCTTCTATTTTGAGTGCAGACCCCAAAGAGACAAAAAGAGTTGGATTGGTTGACCCGATATAAGATTATTAATGGGATTGTTCGGGGAATTTTATATCTTCATGAAGATTCCCGACTTCGAATTATCCATCGTGGTCTTAAAGCTAGTAATGTTTTATTGGATGAAAATATGAATCCGAAAGTTTCAGACTTCGGTATGGCACGAATTATTCAAGTGGATCAGAATGAAGCAAATACAAATCGAATTGCCTAGTAAGTTTTCCTTCTATCCCAAAGCTTGTTTGAAATCATTTATGCTCAATGATCGAGTAATTATAATAAATATGGGATAATTTTTACTTTGTAACTTAAAACTTGAATGTTGATTATTAATAATTCTACATTTCAGCGGTTACATGTCTCCTGAATATGCAATGCACGGAAACTTCTTTACAAAATCTGATGTGTATAGCTTTGGAGTCTTAGTGCTGGAGATTATAAGCAGCAAGAAAAACAATACATTTTATCTATCGGGTCTTTCGAAAGACATCTTGACCTATGTAAGTCAATGATGAAACTTTTCAATTCTTTCTCTTAGCAATTAAATTGAACTTAGCAAATGATGTATTACGCTTAACTCTCAAAATTATTATTTTTTTCGTCAGACATGGAAACTATGGAAGGACGAAATACCACTAGAACTATTAGACCCGAGTTTGGGAGACAACTACTTAAGAAATGAGGTACTTAGATGCATTCATATAGCTTTACTATGCGTGCAAGAGGATCCTAGCTGTAGACCTTCCATGGCATCCATTGTTCTTATGTTGAATAGTTATTCGTTTCCTTTACCTCTTCCTAATGAGCCAGCATCCTTCATACGAAATACAGACAATAACAGTAATCAATCTGCAAGTAGGTCAGTCCAATGGTCTGCCAATAAAATATCCATAATTGAGTTGGATCCTGGTTGA

mRNA sequence

ATGGCTGCAACAAAGAGCGTGGTCTTGGGCATACTCAATTTTCTTAGTTTCATCTTCCTCCCTAGTTTCATCACCGCTCAGACTCTATTACAATACCCATATCGCAGTTGCTTACCCGGCAATTTCACCCAGAACAGCGCCTATCAGTCCAGCCTCAATCTTCTGTTTTTAGAATTCAACAACGTCGCCCACCAAAAAACGTTCCACAGTACGTCCGTTGGTCTGACATCAACCGACACCGTTTACGGTCTCTTCCAGTGCTGGGAAGACGTCACCGACAACTGTGGAGCTTGCGTGGCCACAGCCATCAACGACACGGCCAACAACTTCTGCCGCTTCAGGAAAGGCGCTGTGAGTTGGTACGACGGCTGCATATTGCGCTATTCCAACCAATCTATCTTTTCCGACCTCTCCACCGTCCCAAATCTCATGCTACTCAACACCGCAGATATAGACGCCGACAAGCCTCGCTTCAACCAACTGGTCATGTCCACGTTTGGGGGCACCGTGGCCCGGGCGGCTGACGCCTCTTCCGTGGCCGAAATGGTTGCAACTCGAGAGGCAAATTTCACATTTCATACACTTTACACATTGGCACAGTGCACCGGCGATTTATCCATTTCCAATTGCCGAGAATGCTTACGACAGGCCATCGTTGCTGTCCCGAGTTGTTGCGCAAATAAACGAGGCGGCAGAGTTTTGTATCCCAGCTGCTACGTTCGTTACGAAATTTACCCCTTTTACCGACTCACCGCCAACAATCCTCCACCAATTCCCCCGTCCACTCCCAAAACTCCACCACTGCCTCCTCCTCCTCATGCTCCCTCAGGGGAAACCAGATCCTCCACTCTCATAATTCTGGTCACTGTGCTTCCAATTACCGTCTCCATTCTGCTTTTCGTCGGCTGGTGTTTCCTACGCCAGAGAGCAAAGAAGAGATACACTACTGTAAAGGAAGATCACAGTGTTGTTAGTGGGATTAGCACTGTGGAGTCTCTGCAATTTGATTTCAATACAATCGATGCCGCTACTAACAAATTCTCAGCAGAGAACAGGATAGGTGAAGGTGGATTTGGTGCAGTCTACAAGGGAAGGCTTGAAAATGGGCAAGAGATAGCAGTGAAAAGACTATCGAGAGGATCTTTGCAAGGCTCTGAAGAGTTCAAGAATGAGACCCCAAAGAGACAAAAAGAGTTGGATTGGTTGACCCGATATAAGATTATTAATGGGATTGTTCGGGGAATTTTATATCTTCATGAAGATTCCCGACTTCGAATTATCCATCGTGGTCTTAAAGCTAGTAATGTTTTATTGGATGAAAATATGAATCCGAAAGTTTCAGACTTCGGTATGGCACGAATTATTCAAGTGGATCAGAATGAAGCAAATACAAATCGAATTGCCTACGGTTACATGTCTCCTGAATATGCAATGCACGGAAACTTCTTTACAAAATCTGATGTGTATAGCTTTGGAGTCTTAGTGCTGGAGATTATAAGCAGCAAGAAAAACAATACATTTTATCTATCGGGTCTTTCGAAAGACATCTTGACCTATACATGGAAACTATGGAAGGACGAAATACCACTAGAACTATTAGACCCGAGTTTGGGAGACAACTACTTAAGAAATGAGGTACTTAGATGCATTCATATAGCTTTACTATGCGTGCAAGAGGATCCTAGCTGTAGACCTTCCATGGCATCCATTGTTCTTATGTTGAATAGTTATTCGTTTCCTTTACCTCTTCCTAATGAGCCAGCATCCTTCATACGAAATACAGACAATAACAGTAATCAATCTGCAAGTAGGTCAGTCCAATGGTCTGCCAATAAAATATCCATAATTGAGTTGGATCCTGGTTGA

Coding sequence (CDS)

ATGGCTGCAACAAAGAGCGTGGTCTTGGGCATACTCAATTTTCTTAGTTTCATCTTCCTCCCTAGTTTCATCACCGCTCAGACTCTATTACAATACCCATATCGCAGTTGCTTACCCGGCAATTTCACCCAGAACAGCGCCTATCAGTCCAGCCTCAATCTTCTGTTTTTAGAATTCAACAACGTCGCCCACCAAAAAACGTTCCACAGTACGTCCGTTGGTCTGACATCAACCGACACCGTTTACGGTCTCTTCCAGTGCTGGGAAGACGTCACCGACAACTGTGGAGCTTGCGTGGCCACAGCCATCAACGACACGGCCAACAACTTCTGCCGCTTCAGGAAAGGCGCTGTGAGTTGGTACGACGGCTGCATATTGCGCTATTCCAACCAATCTATCTTTTCCGACCTCTCCACCGTCCCAAATCTCATGCTACTCAACACCGCAGATATAGACGCCGACAAGCCTCGCTTCAACCAACTGGTCATGTCCACGTTTGGGGGCACCGTGGCCCGGGCGGCTGACGCCTCTTCCGTGGCCGAAATGGTTGCAACTCGAGAGGCAAATTTCACATTTCATACACTTTACACATTGGCACAGTGCACCGGCGATTTATCCATTTCCAATTGCCGAGAATGCTTACGACAGGCCATCGTTGCTGTCCCGAGTTGTTGCGCAAATAAACGAGGCGGCAGAGTTTTGTATCCCAGCTGCTACGTTCGTTACGAAATTTACCCCTTTTACCGACTCACCGCCAACAATCCTCCACCAATTCCCCCGTCCACTCCCAAAACTCCACCACTGCCTCCTCCTCCTCATGCTCCCTCAGGGGAAACCAGATCCTCCACTCTCATAATTCTGGTCACTGTGCTTCCAATTACCGTCTCCATTCTGCTTTTCGTCGGCTGGTGTTTCCTACGCCAGAGAGCAAAGAAGAGATACACTACTGTAAAGGAAGATCACAGTGTTGTTAGTGGGATTAGCACTGTGGAGTCTCTGCAATTTGATTTCAATACAATCGATGCCGCTACTAACAAATTCTCAGCAGAGAACAGGATAGGTGAAGGTGGATTTGGTGCAGTCTACAAGGGAAGGCTTGAAAATGGGCAAGAGATAGCAGTGAAAAGACTATCGAGAGGATCTTTGCAAGGCTCTGAAGAGTTCAAGAATGAGACCCCAAAGAGACAAAAAGAGTTGGATTGGTTGACCCGATATAAGATTATTAATGGGATTGTTCGGGGAATTTTATATCTTCATGAAGATTCCCGACTTCGAATTATCCATCGTGGTCTTAAAGCTAGTAATGTTTTATTGGATGAAAATATGAATCCGAAAGTTTCAGACTTCGGTATGGCACGAATTATTCAAGTGGATCAGAATGAAGCAAATACAAATCGAATTGCCTACGGTTACATGTCTCCTGAATATGCAATGCACGGAAACTTCTTTACAAAATCTGATGTGTATAGCTTTGGAGTCTTAGTGCTGGAGATTATAAGCAGCAAGAAAAACAATACATTTTATCTATCGGGTCTTTCGAAAGACATCTTGACCTATACATGGAAACTATGGAAGGACGAAATACCACTAGAACTATTAGACCCGAGTTTGGGAGACAACTACTTAAGAAATGAGGTACTTAGATGCATTCATATAGCTTTACTATGCGTGCAAGAGGATCCTAGCTGTAGACCTTCCATGGCATCCATTGTTCTTATGTTGAATAGTTATTCGTTTCCTTTACCTCTTCCTAATGAGCCAGCATCCTTCATACGAAATACAGACAATAACAGTAATCAATCTGCAAGTAGGTCAGTCCAATGGTCTGCCAATAAAATATCCATAATTGAGTTGGATCCTGGTTGA

Protein sequence

MAATKSVVLGILNFLSFIFLPSFITAQTLLQYPYRSCLPGNFTQNSAYQSSLNLLFLEFNNVAHQKTFHSTSVGLTSTDTVYGLFQCWEDVTDNCGACVATAINDTANNFCRFRKGAVSWYDGCILRYSNQSIFSDLSTVPNLMLLNTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMVATREANFTFHTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYEIYPFYRLTANNPPPIPPSTPKTPPLPPPPHAPSGETRSSTLIILVTVLPITVSILLFVGWCFLRQRAKKRYTTVKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGGFGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNETPKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNVLLDENMNPKVSDFGMARIIQVDQNEANTNRIAYGYMSPEYAMHGNFFTKSDVYSFGVLVLEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIALLCVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIRNTDNNSNQSASRSVQWSANKISIIELDPG
Homology
BLAST of Moc03g28140.1 vs. NCBI nr
Match: XP_022139941.1 (cysteine-rich receptor-like protein kinase 10 [Momordica charantia])

HSP 1 Score: 809.7 bits (2090), Expect = 1.8e-230
Identity = 447/671 (66.62%), Postives = 500/671 (74.52%), Query Frame = 0

Query: 11  ILNFLSFIFLPSFI-----TAQTLLQYPYRSCLPGNFTQNSAYQSSLNLLFLEFN-NVAH 70
           +LN +SFIFL S +      AQT + YPYRSCLP NFTQNS YQSSLNLLF   + N   
Sbjct: 1   MLNSVSFIFLLSLVLIIHGNAQTPVPYPYRSCLPANFTQNSTYQSSLNLLFSNLSANAPG 60

Query: 71  QKTFHSTSVGLTSTDTVYGLFQCWEDVTD-NCGACVATAINDTANNFCRFRKGAVSWYDG 130
              F +TSVG T  DTVYGLFQC  DVT+  C  CVA+   + A  FC  RK AV W D 
Sbjct: 61  INGFFNTSVGRTPNDTVYGLFQCRGDVTNTTCKNCVASTARNVAQQFCPLRKAAVVWDDD 120

Query: 131 CILRYSNQSIFSDLSTVPNLMLLNTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMV 190
           CI RYS+QS FS +ST P+L LLNTADIDADK  FNQLVMST   T ARAA+A++V E+ 
Sbjct: 121 CIFRYSDQSFFSAVSTDPDLTLLNTADIDADKAGFNQLVMSTLRATAARAANATAVGELF 180

Query: 191 ATREANFTF-HTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRY 250
           AT++ANFT   TLYTLAQCTGDLS SNC +CLRQA+ A+P CC+NK+GGRVLYPSCYVRY
Sbjct: 181 ATQQANFTSERTLYTLAQCTGDLSNSNCEDCLRQAVGAIPGCCSNKQGGRVLYPSCYVRY 240

Query: 251 EIYPFYRLTANNPPPIPPSTP----KTPPLPPPPHAPSGETRSSTLIILVTVLPITVSIL 310
           EIY FY LT  NPPP PP+TP     TPP PPPP  P GE RSS ++I+  V PITVSIL
Sbjct: 241 EIYSFYDLTRANPPP-PPATPPPNSTTPPPPPPP--PPGEHRSSIVLIVAIVAPITVSIL 300

Query: 311 LFV-GWCFLRQRAKKRYTTVKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGG 370
           LFV GWCFLR+RAKKR++  KED SVV+ ++TVESLQFDFNTIDAATNKFS ENR+GEGG
Sbjct: 301 LFVLGWCFLRRRAKKRHSAAKED-SVVNEMTTVESLQFDFNTIDAATNKFSEENRLGEGG 360

Query: 371 FGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNET------------------------- 430
           FGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNE                          
Sbjct: 361 FGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNEVMLVAKLQHRNLVRLLGFCLEGEEKI 420

Query: 431 -----------------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASN 490
                            P+ Q+ELDWL RYKII+GI RGILYLHEDSRLRIIHR LK SN
Sbjct: 421 LIYEYIPNKSLDYFLFDPEGQRELDWLKRYKIIHGIARGILYLHEDSRLRIIHRDLKVSN 480

Query: 491 VLLDENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGV 550
           +LLD +MNPK+SDFGM RI QVDQ + NT+RI   YGYMSPEYAMHGNF  KSDVYSFGV
Sbjct: 481 ILLDGDMNPKISDFGMGRIFQVDQTQGNTSRIVGTYGYMSPEYAMHGNFSIKSDVYSFGV 540

Query: 551 LVLEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIA 610
           LVLEI+S KKNNTFYLS L++DILTY WKLWK+ IPLELLDPSL DNY RNEVLRCIHIA
Sbjct: 541 LVLEILSGKKNNTFYLSDLAEDILTYAWKLWKEGIPLELLDPSLRDNYSRNEVLRCIHIA 600

Query: 611 LLCVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIRNTDN----NSNQSASRSVQWS 621
           LLCVQEDP  RPSM SIVLMLNSYS  LPLP EPA F+R  D+    +S+QS S+S+QWS
Sbjct: 601 LLCVQEDPGSRPSMTSIVLMLNSYSVTLPLPEEPAFFMRTKDHGIIIDSDQSMSKSIQWS 660

BLAST of Moc03g28140.1 vs. NCBI nr
Match: XP_038893612.1 (cysteine-rich receptor-like protein kinase 10 isoform X2 [Benincasa hispida])

HSP 1 Score: 750.4 bits (1936), Expect = 1.3e-212
Identity = 421/668 (63.02%), Postives = 476/668 (71.26%), Query Frame = 0

Query: 11  ILNFLSFIFLPSFI---TAQTLLQYPYRSCLPGNFTQNSAYQSSLNLLFLEFN-NVAHQK 70
           +LNFL+F+F  +F+     QT++  PYRSCL GNFT NS YQS+LNLLF   + N   + 
Sbjct: 1   MLNFLTFLFFLNFLIHGKPQTVVSDPYRSCLSGNFTHNSTYQSNLNLLFSALSTNGPPRN 60

Query: 71  TFHSTSVGLTSTDTVYGLFQCWEDVTDN-CGACVATAINDTANNFCRFRKGAVSWYDGCI 130
            F +TS G    DTVYGLFQC  DV D  C   +ATA  D    +C  RKGAV WYD  I
Sbjct: 61  RFFNTSFGRPPNDTVYGLFQCRGDVADAVCRTVLATATTDAERLYCPLRKGAVIWYDEII 120

Query: 131 LRYSNQSIFSDLSTVPNLMLLNTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMVAT 190
            RYS+Q  FS +S +PNL LLNT DID DK RFNQLVMST   T A+AADASSV  + AT
Sbjct: 121 FRYSDQPFFSVISLIPNLQLLNTGDIDVDKARFNQLVMSTLRATAAQAADASSVGGLFAT 180

Query: 191 REANFTFH-TLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYEI 250
           + ANFT    LYTLAQCTGDLS ++C ECLRQA   +PSCC NKRGGRVL PSCYVRYE+
Sbjct: 181 QMANFTGDLILYTLAQCTGDLSNTDCEECLRQATNGIPSCCTNKRGGRVLLPSCYVRYEV 240

Query: 251 YPFYRLTANN--PPPIPPSTPKTPPLPP--PPHAPSGETRSSTLIILVTVLPITVSILLF 310
           Y FY LT  N  PPP  P+     P P   P   PSGE RSST++I+  V PI VSILLF
Sbjct: 241 YMFYELTPTNSVPPPATPTPNSRTPTPARLPSSPPSGERRSSTVLIVAIVAPIAVSILLF 300

Query: 311 -VGWCFLRQRAKKRYTTVKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGGFG 370
            VG C LR+RAKKRY  VKED SVV  ++TVESLQFDF TI+AATNKFS ENR+GEGGFG
Sbjct: 301 VVGCCLLRRRAKKRYFAVKED-SVVDEMTTVESLQFDFKTIEAATNKFSEENRLGEGGFG 360

Query: 371 AVYKGRLENGQEIAVKRLSRGSLQGSEEFKNET--------------------------- 430
           AV+KGRLENGQEIAVKRLSRGSLQGSEEFKNE                            
Sbjct: 361 AVFKGRLENGQEIAVKRLSRGSLQGSEEFKNEVMLVAKLQHRNLVRLVGFCLEGEEKILI 420

Query: 431 ---------------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNVL 490
                          P+ QK+L+WL RYKIINGI RGILYLHEDSRLRIIHR LKASN+L
Sbjct: 421 YEYIPNKSLDFFLFDPEGQKQLNWLKRYKIINGIARGILYLHEDSRLRIIHRDLKASNIL 480

Query: 491 LDENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVLV 550
           LD +MN K+SDFGMARIIQ+DQ + NT+RI   YGYMSPEYAMHGNF  KSDVYSFGVLV
Sbjct: 481 LDGDMNAKISDFGMARIIQMDQTQGNTSRIVGTYGYMSPEYAMHGNFSMKSDVYSFGVLV 540

Query: 551 LEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIALL 610
           LEI+S +KNNTFYLS L++DILTY WKLWKD  PL+LLDP L D Y RNEVLRCIHIALL
Sbjct: 541 LEILSGRKNNTFYLSDLAEDILTYAWKLWKDGTPLDLLDPLLSDTYSRNEVLRCIHIALL 600

Query: 611 CVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIRNTDN---NSNQSASRSVQWSANK 621
           CVQED   RPSMASIVLMLNSYS  LPLP EPA F+R+ DN   +++ S S+SV+ S N+
Sbjct: 601 CVQEDSKSRPSMASIVLMLNSYSVTLPLPEEPAFFMRSKDNGITDTDHSTSKSVKCSVNQ 660

BLAST of Moc03g28140.1 vs. NCBI nr
Match: XP_022933188.1 (cysteine-rich receptor-like protein kinase 10 [Cucurbita moschata])

HSP 1 Score: 749.6 bits (1934), Expect = 2.2e-212
Identity = 421/669 (62.93%), Postives = 488/669 (72.94%), Query Frame = 0

Query: 11  ILNFLSFIFLPSFI---TAQTLLQYPYRSCLPGNFTQNSAYQSSLNLLFLEFN-NVAHQK 70
           +LNFL+F+FL  F+    AQT + YPYRSCLPGNFTQNS Y S+L+LLF   + N   + 
Sbjct: 1   MLNFLTFLFLLCFLIHGNAQTRVSYPYRSCLPGNFTQNSTYHSNLDLLFSNLSANGPPKN 60

Query: 71  TFHSTSVGLTSTDTVYGLFQCWEDVTDN-CGACVATAINDTANNFCRFRKGAVSWYDGCI 130
            F +TS G    D VYGLFQC  DV+D+ C + +ATAI D A  +C  RKGAV+WYD CI
Sbjct: 61  RFFNTSAGRPPNDAVYGLFQCRGDVSDSVCRSFLATAIKDVAQEYCPLRKGAVAWYDECI 120

Query: 131 LRYSNQSIFSDLSTVPNLMLLNTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMVAT 190
            RYS+Q  FSD+ST P+L LLNTA+ID DK RFNQLVMS    T ARAA+A SV E+ AT
Sbjct: 121 FRYSDQRFFSDVSTEPSLPLLNTAEIDVDKARFNQLVMSMLRATAARAANA-SVGELFAT 180

Query: 191 REANFTFH-TLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYEI 250
           +EANFT    LYTLAQCTGDLS ++C  CLRQAI  +PSCC+NKRGGRVL+PSCYVRYE+
Sbjct: 181 QEANFTSDLPLYTLAQCTGDLSNTDCELCLRQAINGIPSCCSNKRGGRVLFPSCYVRYEV 240

Query: 251 YPFYRLTANNPPPIPPSTP-----KTPPLPPPPHAPSGETRSSTLIILVTVLPITVSILL 310
           Y FY  T  N  P P +TP      TPPLPPPP    G+ R ST++I+  V PIT+SILL
Sbjct: 241 YMFYEQTPTNSVPPPATTPPNNTTPTPPLPPPP--TPGKRRISTMLIVAIVAPITISILL 300

Query: 311 FV-GWCFLRQRAKKRYTTVKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGGF 370
           FV G CFLRQR +KR++ VKED SVV+ ++T+ESLQFDF TIDAATNKFS EN++GEGGF
Sbjct: 301 FVLGCCFLRQRVRKRHSAVKED-SVVNEMTTMESLQFDFKTIDAATNKFSEENKLGEGGF 360

Query: 371 GAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNET-------------------------- 430
           G V+KGRLENG+EIAVKRLSRGSLQGSEEFKNE                           
Sbjct: 361 GVVFKGRLENGEEIAVKRLSRGSLQGSEEFKNEVMLVAQLQHRNLVRLLGFCLEGEEKIL 420

Query: 431 -----PKR-----------QKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNV 490
                P +           Q +LDWL RYKIINGI RG+LYLHEDSRLRIIHR LKASN+
Sbjct: 421 IYEYIPNKSLDFFLFDLEGQTQLDWLKRYKIINGIARGMLYLHEDSRLRIIHRDLKASNI 480

Query: 491 LLDENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVL 550
           LLDE MN K+SDFGMARIIQVD+++ NT RI   YGYMSPEYAMHG F  KSDVYSFGVL
Sbjct: 481 LLDEYMNAKISDFGMARIIQVDESQVNTKRIVGTYGYMSPEYAMHGIFSMKSDVYSFGVL 540

Query: 551 VLEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIAL 610
           VLEI+S +KN++FYLS L++D+LTY WKLWK E PLE+LDP L ++Y RNEVLRCIHIAL
Sbjct: 541 VLEILSGQKNSSFYLSDLAEDLLTYAWKLWKTEQPLEILDPVLRNSYSRNEVLRCIHIAL 600

Query: 611 LCVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIR--NTD-NNSNQSASRSVQWSAN 621
           LCVQED S RPSMASIVLMLNS S  LPLP EPA F+R  NTD  +S+ S  R VQWS N
Sbjct: 601 LCVQEDSSQRPSMASIVLMLNSNSVTLPLPEEPAFFMRSKNTDIKDSDISTDRFVQWSVN 660

BLAST of Moc03g28140.1 vs. NCBI nr
Match: XP_023001238.1 (cysteine-rich receptor-like protein kinase 10 [Cucurbita maxima])

HSP 1 Score: 746.9 bits (1927), Expect = 1.4e-211
Identity = 415/669 (62.03%), Postives = 486/669 (72.65%), Query Frame = 0

Query: 11  ILNFLSFIFLPSFI---TAQTLLQYPYRSCLPGNFTQNSAYQSSLNLLFLEFN-NVAHQK 70
           +LNF++F+FL  F+    AQT + YPYRSCLPGNFTQNS Y S+LNLLF   + N     
Sbjct: 1   MLNFVTFLFLLCFLIHGNAQTPVPYPYRSCLPGNFTQNSTYHSNLNLLFSNLSANAPPNN 60

Query: 71  TFHSTSVGLTSTDTVYGLFQCWEDVTDN-CGACVATAINDTANNFCRFRKGAVSWYDGCI 130
            F +TS G    D VYGLFQC  DV+D+ C + +ATAI D A  +C  R GAV+WYD CI
Sbjct: 61  RFFNTSAGRPPNDAVYGLFQCRGDVSDSVCRSFLATAIKDVAEKYCPLRNGAVAWYDECI 120

Query: 131 LRYSNQSIFSDLSTVPNLMLLNTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMVAT 190
            RYS+Q  FSD+ST P+L LLNT +ID DK RFNQLVMST   T ARAA+A SV E+ AT
Sbjct: 121 FRYSDQPFFSDVSTDPSLSLLNTDEIDVDKARFNQLVMSTLRATAARAANA-SVGELFAT 180

Query: 191 REANFTFH-TLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYEI 250
           +EANFT    L+TLAQCTGDLS ++C  CLRQAI  +PSCC+NKRGGRVL+PSCYVRYE+
Sbjct: 181 QEANFTSDLPLHTLAQCTGDLSNTDCELCLRQAINGIPSCCSNKRGGRVLFPSCYVRYEV 240

Query: 251 YPFYRLTANNPPPIPPSTP-----KTPPLPPPPHAPSGETRSSTLIILVTVLPITVSILL 310
           Y FY  T  N  P P +TP      TPPLPPPP  P G+ R ST++I+  V PIT+SILL
Sbjct: 241 YMFYEQTLTNSVPPPATTPPNNTTPTPPLPPPPPTP-GKRRISTMLIVAIVAPITISILL 300

Query: 311 FV-GWCFLRQRAKKRYTTVKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGGF 370
           FV G CFLRQRA+KR++TVKED SVV+ ++TVESL FDF TID+ATNKFS EN++GEGGF
Sbjct: 301 FVLGCCFLRQRARKRHSTVKED-SVVNEMTTVESLLFDFKTIDSATNKFSEENKLGEGGF 360

Query: 371 GAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNET-------------------------- 430
           G V+KGR ENG+EIAVKRLSRGSLQGSEEFKNE                           
Sbjct: 361 GVVFKGRFENGEEIAVKRLSRGSLQGSEEFKNEVLLVAQLQHRNLVRLLGFCLEGEEKIL 420

Query: 431 ----------------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNV 490
                            +RQ  LDWL RYKIINGI RG+LYLHEDSRLRIIHR LKASN+
Sbjct: 421 IYEYIPNKSLDFFLFDSERQTRLDWLKRYKIINGIARGMLYLHEDSRLRIIHRDLKASNI 480

Query: 491 LLDENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVL 550
           LLDE MN K+SDFGMARIIQVD+++ NT RI   YGYMSPEYAMHG F  KSDVYSFGVL
Sbjct: 481 LLDEYMNAKISDFGMARIIQVDESQVNTKRIVGTYGYMSPEYAMHGIFSMKSDVYSFGVL 540

Query: 551 VLEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIAL 610
           VLEI+S +KN++FYLS L++D+LTY WKLWK E PLE+L P L + Y RNEVL+CIHIAL
Sbjct: 541 VLEILSGQKNSSFYLSDLAEDLLTYAWKLWKTEQPLEILHPVLRNCYSRNEVLKCIHIAL 600

Query: 611 LCVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIR--NTD-NNSNQSASRSVQWSAN 621
           LCVQEDPS RPSM SIVLML+S S  +PLP EPA F+R  NTD  NS+ S ++ VQWS N
Sbjct: 601 LCVQEDPSQRPSMTSIVLMLSSNSVTMPLPEEPAFFLRSKNTDIKNSDISTNKFVQWSVN 660

BLAST of Moc03g28140.1 vs. NCBI nr
Match: XP_023519606.1 (cysteine-rich receptor-like protein kinase 10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 742.3 bits (1915), Expect = 3.5e-210
Identity = 413/669 (61.73%), Postives = 487/669 (72.80%), Query Frame = 0

Query: 11  ILNFLSFIFLPSFI---TAQTLLQYPYRSCLPGNFTQNSAYQSSLNLLFLEFN-NVAHQK 70
           +LNF++F+F+  F+    AQ  + YPYRSCLPGNFTQNS Y S+L+LLF   + N   + 
Sbjct: 1   MLNFVTFLFILCFLIHGNAQIPVSYPYRSCLPGNFTQNSTYHSNLDLLFSNLSANGPPKN 60

Query: 71  TFHSTSVGLTSTDTVYGLFQCWEDVTDN-CGACVATAINDTANNFCRFRKGAVSWYDGCI 130
            F +TS G    D VYGLFQC  DV+D+ C + +ATAI D A  +C   KGAV+WYD CI
Sbjct: 61  RFFNTSAGRPPNDAVYGLFQCRGDVSDSGCRSLLATAIKDVAREYCPLSKGAVAWYDECI 120

Query: 131 LRYSNQSIFSDLSTVPNLMLLNTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMVAT 190
           LRYS+Q  FSD+ST P++ LLNTA+ID DK  FNQLVMST   T ARAA+A SV E+ AT
Sbjct: 121 LRYSDQPFFSDVSTKPSMSLLNTAEIDVDKAPFNQLVMSTLRATAARAANA-SVGELFAT 180

Query: 191 REANFTFH-TLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYEI 250
           +EANFT   TLYTLAQCTGDLS ++C  CLRQAI  +PSCC+NKRGGRVL+PSCYVRYE+
Sbjct: 181 QEANFTSDLTLYTLAQCTGDLSNTDCELCLRQAINGIPSCCSNKRGGRVLFPSCYVRYEV 240

Query: 251 YPFYRLTANNPPPIPPSTP-----KTPPLPPPPHAPSGETRSSTLIILVTVLPITVSILL 310
           Y FY  T  N  P P +TP      TPPLPPPP    G+ R ST++I+  V PIT+SILL
Sbjct: 241 YMFYEQTPTNSVPPPATTPPNNTTPTPPLPPPP--TPGKRRISTMLIVAIVAPITISILL 300

Query: 311 FV-GWCFLRQRAKKRYTTVKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGGF 370
           FV G CFLRQRA+ R++ VKED SVV+ ++T+ESLQFDF TID+ATNKFS EN++GEGGF
Sbjct: 301 FVLGCCFLRQRARNRHSPVKED-SVVNEMTTMESLQFDFKTIDSATNKFSEENKLGEGGF 360

Query: 371 GAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNET-------------------------- 430
           G V+KGRLENG+EIAVKRLSRGSLQGSEEFKNE                           
Sbjct: 361 GVVFKGRLENGEEIAVKRLSRGSLQGSEEFKNEVMLVAQLQHRNLVRLLGFCLEGEEKIL 420

Query: 431 -----PKR-----------QKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNV 490
                P +           Q +LDWL RYKIINGI RG+LYLHEDSRLRIIHR LKASN+
Sbjct: 421 IYEYIPNKSLDFFLFDLEGQTQLDWLKRYKIINGIARGMLYLHEDSRLRIIHRDLKASNI 480

Query: 491 LLDENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVL 550
           LLDE MN K+SDFGMARIIQVD+++ NT RI   YGYMSPEYAMHG F  KSDVYSFGVL
Sbjct: 481 LLDEYMNAKISDFGMARIIQVDESQVNTKRIVGTYGYMSPEYAMHGIFSMKSDVYSFGVL 540

Query: 551 VLEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIAL 610
           VLEI+S +KN++FYLS L++D+LTY WKLWK E PLE+LDP L ++Y RNEVLRCIHIAL
Sbjct: 541 VLEILSGQKNSSFYLSDLAEDLLTYAWKLWKTEQPLEILDPVLRNSYSRNEVLRCIHIAL 600

Query: 611 LCVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIRNTDNN---SNQSASRSVQWSAN 621
           LCVQED S RP+MASIVLMLNS S  LPLP EPA F+R+ D N   S+ S ++  QWS N
Sbjct: 601 LCVQEDSSQRPAMASIVLMLNSNSVTLPLPEEPAFFMRSKDTNIKDSDISTNQFAQWSVN 660

BLAST of Moc03g28140.1 vs. ExPASy Swiss-Prot
Match: Q8GYA4 (Cysteine-rich receptor-like protein kinase 10 OS=Arabidopsis thaliana OX=3702 GN=CRK10 PE=1 SV=3)

HSP 1 Score: 487.6 bits (1254), Expect = 2.0e-136
Identity = 295/676 (43.64%), Postives = 410/676 (60.65%), Query Frame = 0

Query: 5   KSVVLGILNFLSFIFLPSFITAQTLL----QYPYRSCL-PGNFTQNSAYQSSLNLLF--L 64
           +S ++   +   F+FL SF+T+  +      Y Y +C    N+T NS Y ++L  L   L
Sbjct: 8   ESPIMSYYSSFFFLFLFSFLTSFRVSAQDPTYVYHTCQNTANYTSNSTYNNNLKTLLASL 67

Query: 65  EFNNVAHQKTFHSTSVGLTSTDTVYGLFQCWEDV-TDNCGACVATAINDTANNFCRFRKG 124
              N ++   F + +VG  + D V GLF C  DV T+ C  CV+ A+NDT    C  +K 
Sbjct: 68  SSRNASYSTGFQNATVG-QAPDRVTGLFNCRGDVSTEVCRRCVSFAVNDTLTR-CPNQKE 127

Query: 125 AVSWYDGCILRYSNQSIFSDLSTVPNLMLLNTADIDADK-PRFNQLVMSTFGGTVARAAD 184
           A  +YD C+LRYSNQ+I S L T   ++L+NT ++ +++    + LV+ T       A +
Sbjct: 128 ATLYYDECVLRYSNQNILSTLITTGGVILVNTRNVTSNQLDLLSDLVLPTLNQAATVALN 187

Query: 185 ASSVAEMVATREANFT-FHTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVL 244
           +S   +   TR+ NFT   + Y L QCT DL+  +C  CL+  I  +P+   ++ G R++
Sbjct: 188 SS---KKFGTRKNNFTALQSFYGLVQCTPDLTRQDCSRCLQLVINQIPT---DRIGARII 247

Query: 245 YPSCYVRYEIYPFYRLTANNPPPIPPSTPKTPPLPPPPHAPSGETRSSTLIILVTVLPIT 304
            PSC  RYEIY FY  +A  PPP PPS   TPP+  PP   SG+  +S ++++  V+PI 
Sbjct: 248 NPSCTSRYEIYAFYTESAVPPPPPPPSI-STPPVSAPPR--SGKDGNSKVLVIAIVVPII 307

Query: 305 VSILLFV-GWCFLRQRAKKRYTTVKEDHSVVSG--ISTVESLQFDFNTIDAATNKFSAEN 364
           V++LLF+ G+CFL +RA+K Y T     S  +G  I+T +SLQ D+ TI  AT+ F   N
Sbjct: 308 VAVLLFIAGYCFLTRRARKSYYT----PSAFAGDDITTADSLQLDYRTIQTATDDFVESN 367

Query: 365 RIGEGGFGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNET------------------- 424
           +IG+GGFG VYKG L +G E+AVKRLS+ S QG  EFKNE                    
Sbjct: 368 KIGQGGFGEVYKGTLSDGTEVAVKRLSKSSGQGEVEFKNEVVLVAKLQHRNLVRLLGFCL 427

Query: 425 -----------------------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHR 484
                                  P ++ +LDW  RYKII G+ RGILYLH+DSRL IIHR
Sbjct: 428 DGEERVLVYEYVPNKSLDYFLFDPAKKGQLDWTRRYKIIGGVARGILYLHQDSRLTIIHR 487

Query: 485 GLKASNVLLDENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSD 544
            LKASN+LLD +MNPK++DFGMARI  +DQ E NT+RI   YGYMSPEYAMHG +  KSD
Sbjct: 488 DLKASNILLDADMNPKIADFGMARIFGLDQTEENTSRIVGTYGYMSPEYAMHGQYSMKSD 547

Query: 545 VYSFGVLVLEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVL 604
           VYSFGVLVLEIIS KKN++FY +  + D+++Y W LW +  PLEL+DP++ +N  RNEV+
Sbjct: 548 VYSFGVLVLEIISGKKNSSFYQTDGAHDLVSYAWGLWSNGRPLELVDPAIVENCQRNEVV 607

Query: 605 RCIHIALLCVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIRN---TDNNSNQSASR 621
           RC+HI LLCVQEDP+ RP++++IVLML S +  LP+P +P  F ++    D     + S+
Sbjct: 608 RCVHIGLLCVQEDPAERPTLSTIVLMLTSNTVTLPVPRQPGLFFQSRIGKDPLDTDTTSK 667

BLAST of Moc03g28140.1 vs. ExPASy Swiss-Prot
Match: Q9M0X5 (Cysteine-rich receptor-like protein kinase 25 OS=Arabidopsis thaliana OX=3702 GN=CRK25 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 6.7e-132
Identity = 278/627 (44.34%), Postives = 375/627 (59.81%), Query Frame = 0

Query: 42  FTQNSAYQSSLN--LLFLEFNNVAHQKTFHSTSVG-LTSTDTVYGLFQCWEDVT-DNCGA 101
           +++NS+Y ++L   L  L   N A+   F + + G    ++ VYG+F C  DV+ + C  
Sbjct: 40  YSRNSSYLTNLRTVLSSLSSPNAAYASLFDNAAAGEENDSNRVYGVFLCRGDVSAEICRD 99

Query: 102 CVATAINDTANNFCRFRKGAVSWYDGCILRYSNQSIFSDLSTVPNLMLLNTADIDADK-P 161
           CVA A N+T    C   K AV WYD C++RYSNQSI   +   P + L N  +I  ++  
Sbjct: 100 CVAFAANETLQR-CPREKVAVIWYDECMVRYSNQSIVGQMRIRPGVFLTNKQNITENQVS 159

Query: 162 RFNQLVMSTFGGTVARAADASSVAEMVATREANFT-FHTLYTLAQCTGDLSISNCRECLR 221
           RFN+ + +       +AA +S      AT +ANFT F T+Y+L QCT DL+  +C  CLR
Sbjct: 160 RFNESLPALLIDVAVKAALSS---RKFATEKANFTVFQTIYSLVQCTPDLTNQDCESCLR 219

Query: 222 QAIVAVPSCCANKRGGRVLYPSCYVRYEIYPFYRLTANNPPPIPP--STPKTPPLPPPPH 281
           Q I  +P CC    GGRV+ PSC  RYE+YPFY  T    P  PP  ST   PPL  P  
Sbjct: 220 QVINYLPRCCDRSVGGRVIAPSCSFRYELYPFYNETIAAAPMAPPPSSTVTAPPLNIP-- 279

Query: 282 APSGETRSSTLIILVTVLPITVSILLFVGWCFLRQRAKKRYTTVKEDHSVVSGISTVESL 341
           +  G+ ++ T+I+    +P++V +LL    C+L  R +    + + +     GI++ E+L
Sbjct: 280 SEKGKGKNLTVIVTAIAVPVSVCVLLLGAMCWLLARRRNNKLSAETEDLDEDGITSTETL 339

Query: 342 QFDFNTIDAATNKFSAENRIGEGGFGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNETP 401
           QF F+ I+AATNKFS  N++G GGFG VYKG+L  G+ +A+KRLS+GS QG+EEFKNE  
Sbjct: 340 QFQFSAIEAATNKFSESNKLGHGGFGEVYKGQLITGETVAIKRLSQGSTQGAEEFKNEVD 399

Query: 402 ------------------------------------------KRQKELDWLTRYKIINGI 461
                                                     ++++ LDW  RYKII GI
Sbjct: 400 VVAKLQHRNLAKLLGYCLDGEEKILVYEFVPNKSLDYFLFDNEKRRVLDWQRRYKIIEGI 459

Query: 462 VRGILYLHEDSRLRIIHRGLKASNVLLDENMNPKVSDFGMARIIQVDQNEANTNRI--AY 521
            RGILYLH DSRL IIHR LKASN+LLD +M+PK+SDFGMARI  VDQ +ANT RI   Y
Sbjct: 460 ARGILYLHRDSRLTIIHRDLKASNILLDADMHPKISDFGMARIFGVDQTQANTKRIVGTY 519

Query: 522 GYMSPEYAMHGNFFTKSDVYSFGVLVLEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIP 581
           GYMSPEYA+HG +  KSDVYSFGVLVLE+I+ KKN++FY      D++TY WKLW +  P
Sbjct: 520 GYMSPEYAIHGKYSVKSDVYSFGVLVLELITGKKNSSFYEEDGLGDLVTYVWKLWVENSP 579

Query: 582 LELLDPSLGDNYLRNEVLRCIHIALLCVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPAS 616
           LEL+D ++  N+  NEV+RCIHIALLCVQED S RPSM  I++M+NS++  LP+P     
Sbjct: 580 LELVDEAMRGNFQTNEVIRCIHIALLCVQEDSSERPSMDDILVMMNSFTVTLPIPKRSGF 639

BLAST of Moc03g28140.1 vs. ExPASy Swiss-Prot
Match: Q9C5S8 (Cysteine-rich receptor-like protein kinase 5 OS=Arabidopsis thaliana OX=3702 GN=CRK5 PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 2.7e-125
Identity = 288/664 (43.37%), Postives = 386/664 (58.13%), Query Frame = 0

Query: 15  LSFIFLPSF----ITAQTLLQYP--YRSCLPGNFTQNSAYQSSLNLLF--LEFNNVAHQK 74
           L+F+FL +F    +     LQ P           ++NS Y S+L  L   L  NN     
Sbjct: 7   LNFLFLLTFFIGSLRVSAQLQDPTYVGHVCTNRISRNSIYFSNLQTLLTSLSSNNAYFSL 66

Query: 75  TFHSTSVGLTSTDTVYGLFQCWEDVT-DNCGACVATAINDTANNFCRFRKGAVSWYDGCI 134
             HS + G  S D V+GL+ C  D++ ++C  CV  A  DT +  C   K  +  YD C+
Sbjct: 67  GSHSLTKGQNS-DMVFGLYLCKGDLSPESCRECVIFAAKDTRSR-CPGGKEFLIQYDECM 126

Query: 135 LRYSNQSIFSDLSTVPNLMLLNTADIDADK-PRFNQLVMSTFGGTVARAADASSVAEMVA 194
           L YS+++IF D  T   ++  NT  + AD+  RFN  V+S    +   AA+++S  +  A
Sbjct: 127 LGYSDRNIFMDTVTTTTIITWNTQKVTADQSDRFNDAVLSLMKKSAEEAANSTS--KKFA 186

Query: 195 TREANF-TFHTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYE 254
            ++++F +  +LY   QC  DL+  +C  CL+Q+I        NK GGR L PSC  RYE
Sbjct: 187 VKKSDFSSSQSLYASVQCIPDLTSEDCVMCLQQSI---KELYFNKVGGRFLVPSCNSRYE 246

Query: 255 IYPFYRLTANNPPPIPPSTPKTPPLPPPPHAPSGETRSSTLIILVTVLPITVSILLFVG- 314
           +YPFY+ T       PP +    PL   P  P G+ ++ST+II+  V+P+ +S+L+ V  
Sbjct: 247 VYPFYKETIEGTVLPPPVSAPPLPLVSTPSFPPGKGKNSTVIIIAIVVPVAISVLICVAV 306

Query: 315 WCF-LRQRAKKRYTTVKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGGFGAV 374
           + F   +RAKK Y T +ED      I+T  SLQFDF  I+AAT+KFS  N++G+GGFG V
Sbjct: 307 FSFHASKRAKKTYDTPEED-----DITTAGSLQFDFKVIEAATDKFSMCNKLGQGGFGQV 366

Query: 375 YKGRLENGQEIAVKRLSRGSLQGSEEFKNET----------------------------- 434
           YKG L NG ++AVKRLS+ S QG +EFKNE                              
Sbjct: 367 YKGTLPNGVQVAVKRLSKTSGQGEKEFKNEVVVVAKLQHRNLVKLLGFCLEREEKILVYE 426

Query: 435 -------------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNVLLD 494
                         + Q +LDW TRYKII GI RGILYLH+DSRL IIHR LKA N+LLD
Sbjct: 427 FVSNKSLDYFLFDSRMQSQLDWTTRYKIIGGIARGILYLHQDSRLTIIHRDLKAGNILLD 486

Query: 495 ENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVLVLE 554
            +MNPKV+DFGMARI ++DQ EA+T R+   YGYMSPEYAM+G F  KSDVYSFGVLVLE
Sbjct: 487 ADMNPKVADFGMARIFEIDQTEAHTRRVVGTYGYMSPEYAMYGQFSMKSDVYSFGVLVLE 546

Query: 555 IISSKKNNTFYLSGLS-KDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIALLC 614
           IIS +KN++ Y    S  +++TYTW+LW D  PL+L+D S  D+Y RNE++RCIHIALLC
Sbjct: 547 IISGRKNSSLYQMDASFGNLVTYTWRLWSDGSPLDLVDSSFRDSYQRNEIIRCIHIALLC 606

Query: 615 VQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIRNTDNNSNQSASRSVQWSANKISII 621
           VQED   RP+M++IV ML + S  L +P  P  F R+    +  S  +S   S +  SI 
Sbjct: 607 VQEDTENRPTMSAIVQMLTTSSIALAVPQPPGFFFRSNHEQAGPSMDKSSLCSIDAASIT 658

BLAST of Moc03g28140.1 vs. ExPASy Swiss-Prot
Match: Q8L7G3 (Cysteine-rich receptor-like protein kinase 7 OS=Arabidopsis thaliana OX=3702 GN=CRK7 PE=2 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 7.7e-120
Identity = 277/669 (41.41%), Postives = 378/669 (56.50%), Query Frame = 0

Query: 17  FIFLPSFIT-----AQTLLQYPYRSCLPGNFTQNSAYQSSLNLLF--LEFNNVAHQKTFH 76
           F+FL SF+T     AQ      Y       ++ NS Y ++L  L   L   N ++   F 
Sbjct: 9   FLFLFSFLTSFRASAQDPRFLAYYCPNATTYSSNSTYLTNLKTLLSSLSSRNASYSTGFQ 68

Query: 77  STSVGLTSTDTVYGLFQCWEDVT-DNCGACVATAINDTANNFCRFRKGAVSWYDGCILRY 136
           + +VG  + D V GLF C  DV+ + C  CV  A+N+T +  C  ++ AV +Y+ CILRY
Sbjct: 69  NATVG-QALDRVTGLFLCRGDVSPEVCRNCVTFAVNNTFSR-CPNQREAVFYYEECILRY 128

Query: 137 SNQSIFSDLSTVPNLMLL----NTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMVA 196
           S+++I S   T     +L    + + I     +F  LV+S        AAD       + 
Sbjct: 129 SHKNILSTAITNEGEFILRNPNHISPIQNQINQFTNLVLSNMNQIAIEAADNPRKFSTIK 188

Query: 197 TREANFTFHTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYEI 256
           T        T Y L QCT DLS  NC  CL  +I  +P    ++ G R  +PSC  RYE+
Sbjct: 189 TELT--ALQTFYGLVQCTPDLSRQNCMNCLTSSINRMP---FSRIGARQFWPSCNSRYEL 248

Query: 257 YPFYRLTANNPPPIPPSTPKTPPLPPPPHAPSGETRSSTLIILVTVLPITVSILLFV-GW 316
           Y FY  TA   PP     P  PPL  P  + S ++ +S ++++  V+PI V++L+F+ G+
Sbjct: 249 YDFYNETAIGTPP-----PPLPPLASP--SLSDKSGNSNVVVVAVVVPIIVAVLIFIAGY 308

Query: 317 CFLRQRAKKRYTT---VKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGGFGA 376
           CF  +RAKK Y T   + ED       +T+ESLQ D+  I AATN FS  N+IG GGFG 
Sbjct: 309 CFFAKRAKKTYGTTPALDEDDK-----TTIESLQLDYRAIQAATNDFSENNKIGRGGFGD 368

Query: 377 VYKGRLENGQEIAVKRLSRGSLQGSEEFKNET---------------------------- 436
           VYKG   NG E+AVKRLS+ S QG  EFKNE                             
Sbjct: 369 VYKGTFSNGTEVAVKRLSKTSEQGDTEFKNEVVVVANLRHKNLVRILGFSIEREERILVY 428

Query: 437 --------------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNVLL 496
                         P ++ +L W  RY II GI RGILYLH+DSRL IIHR LKASN+LL
Sbjct: 429 EYVENKSLDNFLFDPAKKGQLYWTQRYHIIGGIARGILYLHQDSRLTIIHRDLKASNILL 488

Query: 497 DENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVLVL 556
           D +MNPK++DFGMARI  +DQ + NT+RI   YGYMSPEYAM G F  KSDVYSFGVLVL
Sbjct: 489 DADMNPKIADFGMARIFGMDQTQQNTSRIVGTYGYMSPEYAMRGQFSMKSDVYSFGVLVL 548

Query: 557 EIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIALLC 616
           EIIS +KNN+F  +  ++D++T+ W+LW++   L+L+DP + D+  ++EV+RC HI LLC
Sbjct: 549 EIISGRKNNSFIETDDAQDLVTHAWRLWRNGTALDLVDPFIADSCRKSEVVRCTHIGLLC 608

Query: 617 VQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIR-----NTDNNSNQSASRSVQWSAN 621
           VQEDP  RP+M++I +ML S +  LP P +P  F+R     N  ++   + ++SV  S +
Sbjct: 609 VQEDPVKRPAMSTISVMLTSNTMALPAPQQPGFFVRSRPGTNRLDSDQSTTNKSVTVSID 658

BLAST of Moc03g28140.1 vs. ExPASy Swiss-Prot
Match: Q8W4G6 (Cysteine-rich receptor-like protein kinase 15 OS=Arabidopsis thaliana OX=3702 GN=CRK15 PE=2 SV=2)

HSP 1 Score: 423.3 bits (1087), Expect = 4.7e-117
Identity = 266/626 (42.49%), Postives = 353/626 (56.39%), Query Frame = 0

Query: 17  FIFLPSFITAQTLLQYPYRSC-LPGNFTQNSAYQSSLNLLF--LEFNNVAHQKTFHSTSV 76
           F FL SF  +   + Y    C     +++NS Y ++L  L   L   N ++   F S   
Sbjct: 11  FFFLTSFTASVENVFYIKHICPNTTTYSRNSPYLTNLRTLLSSLSAPNASYSTGFQSARA 70

Query: 77  GLTSTDTVYGLFQCWEDVTDN-CGACVATAINDTANNFCRFRKGAVSWYDGCILRYSNQS 136
           G  + D V GLF C  DV+   C  CVA +INDT    C   + +V +YD C+LRYS+Q+
Sbjct: 71  G-QAPDRVTGLFLCRGDVSPAVCRNCVAFSINDTLVQ-CPSERKSVFYYDECMLRYSDQN 130

Query: 137 IFSDLSTVPNLMLLN-TADIDADK-PRFNQLVMSTFGGTVARAADASSVAEMVATREANF 196
           I S L+     + +N    ID ++  RF   V ST      +A  ASS  +    +    
Sbjct: 131 ILSTLAYDGAWIRMNGNISIDQNQMNRFKDFVSSTMNQAAVKA--ASSPRKFYTVKATWT 190

Query: 197 TFHTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYEIYPFYR- 256
              TLY L QCT DL+  +C  CL  +I  +P     K GGR LY SC  RYE++ FY  
Sbjct: 191 ALQTLYGLVQCTPDLTRQDCFSCLESSIKLMP---LYKTGGRTLYSSCNSRYELFAFYNE 250

Query: 257 ---LTANNPPPIPPSTPKTPPLPPPPHAPSGETRSSTLIILVTVLPITVSILLFVGWCFL 316
               T   PPP+PPS   + PL   P  P     S+ L++ + +  +  ++LL  G+CF 
Sbjct: 251 TTVRTQQAPPPLPPS---STPLVTSPSLPGKSWNSNVLVVAIVLTILVAALLLIAGYCF- 310

Query: 317 RQRAKKRYTTVKEDHSVVSGIS-TVESLQFDFNTIDAATNKFSAENRIGEGGFGAVYKGR 376
                KR     ++     G   T ESLQ D+  I AATNKFS  N+IG+GGFG VYKG 
Sbjct: 311 ----AKRVKNSSDNAPAFDGDDITTESLQLDYRMIRAATNKFSENNKIGQGGFGEVYKGT 370

Query: 377 LENGQEIAVKRLSRGSLQGSEEFKNET--------------------------------- 436
             NG E+AVKRLS+ S QG  EFKNE                                  
Sbjct: 371 FSNGTEVAVKRLSKSSGQGDTEFKNEVVVVAKLQHRNLVRLLGFSIGGGERILVYEYMPN 430

Query: 437 ---------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNVLLDENMN 496
                    P +Q +LDW  RYK+I GI RGILYLH+DSRL IIHR LKASN+LLD +MN
Sbjct: 431 KSLDYFLFDPAKQNQLDWTRRYKVIGGIARGILYLHQDSRLTIIHRDLKASNILLDADMN 490

Query: 497 PKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVLVLEIISS 556
           PK++DFG+ARI  +DQ + NT+RI   +GYM+PEYA+HG F  KSDVYSFGVLVLEIIS 
Sbjct: 491 PKLADFGLARIFGMDQTQENTSRIVGTFGYMAPEYAIHGQFSVKSDVYSFGVLVLEIISG 550

Query: 557 KKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIALLCVQEDP 588
           KKNN+FY +  + D++T+ W+LW +   L+L+DP + DN  ++EV+RCIHI LLCVQEDP
Sbjct: 551 KKNNSFYETDGAHDLVTHAWRLWSNGTALDLVDPIIIDNCQKSEVVRCIHICLLCVQEDP 610

BLAST of Moc03g28140.1 vs. ExPASy TrEMBL
Match: A0A6J1CE69 (cysteine-rich receptor-like protein kinase 10 OS=Momordica charantia OX=3673 GN=LOC111010730 PE=4 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 8.6e-231
Identity = 447/671 (66.62%), Postives = 500/671 (74.52%), Query Frame = 0

Query: 11  ILNFLSFIFLPSFI-----TAQTLLQYPYRSCLPGNFTQNSAYQSSLNLLFLEFN-NVAH 70
           +LN +SFIFL S +      AQT + YPYRSCLP NFTQNS YQSSLNLLF   + N   
Sbjct: 1   MLNSVSFIFLLSLVLIIHGNAQTPVPYPYRSCLPANFTQNSTYQSSLNLLFSNLSANAPG 60

Query: 71  QKTFHSTSVGLTSTDTVYGLFQCWEDVTD-NCGACVATAINDTANNFCRFRKGAVSWYDG 130
              F +TSVG T  DTVYGLFQC  DVT+  C  CVA+   + A  FC  RK AV W D 
Sbjct: 61  INGFFNTSVGRTPNDTVYGLFQCRGDVTNTTCKNCVASTARNVAQQFCPLRKAAVVWDDD 120

Query: 131 CILRYSNQSIFSDLSTVPNLMLLNTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMV 190
           CI RYS+QS FS +ST P+L LLNTADIDADK  FNQLVMST   T ARAA+A++V E+ 
Sbjct: 121 CIFRYSDQSFFSAVSTDPDLTLLNTADIDADKAGFNQLVMSTLRATAARAANATAVGELF 180

Query: 191 ATREANFTF-HTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRY 250
           AT++ANFT   TLYTLAQCTGDLS SNC +CLRQA+ A+P CC+NK+GGRVLYPSCYVRY
Sbjct: 181 ATQQANFTSERTLYTLAQCTGDLSNSNCEDCLRQAVGAIPGCCSNKQGGRVLYPSCYVRY 240

Query: 251 EIYPFYRLTANNPPPIPPSTP----KTPPLPPPPHAPSGETRSSTLIILVTVLPITVSIL 310
           EIY FY LT  NPPP PP+TP     TPP PPPP  P GE RSS ++I+  V PITVSIL
Sbjct: 241 EIYSFYDLTRANPPP-PPATPPPNSTTPPPPPPP--PPGEHRSSIVLIVAIVAPITVSIL 300

Query: 311 LFV-GWCFLRQRAKKRYTTVKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGG 370
           LFV GWCFLR+RAKKR++  KED SVV+ ++TVESLQFDFNTIDAATNKFS ENR+GEGG
Sbjct: 301 LFVLGWCFLRRRAKKRHSAAKED-SVVNEMTTVESLQFDFNTIDAATNKFSEENRLGEGG 360

Query: 371 FGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNET------------------------- 430
           FGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNE                          
Sbjct: 361 FGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNEVMLVAKLQHRNLVRLLGFCLEGEEKI 420

Query: 431 -----------------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASN 490
                            P+ Q+ELDWL RYKII+GI RGILYLHEDSRLRIIHR LK SN
Sbjct: 421 LIYEYIPNKSLDYFLFDPEGQRELDWLKRYKIIHGIARGILYLHEDSRLRIIHRDLKVSN 480

Query: 491 VLLDENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGV 550
           +LLD +MNPK+SDFGM RI QVDQ + NT+RI   YGYMSPEYAMHGNF  KSDVYSFGV
Sbjct: 481 ILLDGDMNPKISDFGMGRIFQVDQTQGNTSRIVGTYGYMSPEYAMHGNFSIKSDVYSFGV 540

Query: 551 LVLEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIA 610
           LVLEI+S KKNNTFYLS L++DILTY WKLWK+ IPLELLDPSL DNY RNEVLRCIHIA
Sbjct: 541 LVLEILSGKKNNTFYLSDLAEDILTYAWKLWKEGIPLELLDPSLRDNYSRNEVLRCIHIA 600

Query: 611 LLCVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIRNTDN----NSNQSASRSVQWS 621
           LLCVQEDP  RPSM SIVLMLNSYS  LPLP EPA F+R  D+    +S+QS S+S+QWS
Sbjct: 601 LLCVQEDPGSRPSMTSIVLMLNSYSVTLPLPEEPAFFMRTKDHGIIIDSDQSMSKSIQWS 660

BLAST of Moc03g28140.1 vs. ExPASy TrEMBL
Match: A0A6J1F417 (cysteine-rich receptor-like protein kinase 10 OS=Cucurbita moschata OX=3662 GN=LOC111440040 PE=4 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 1.1e-212
Identity = 421/669 (62.93%), Postives = 488/669 (72.94%), Query Frame = 0

Query: 11  ILNFLSFIFLPSFI---TAQTLLQYPYRSCLPGNFTQNSAYQSSLNLLFLEFN-NVAHQK 70
           +LNFL+F+FL  F+    AQT + YPYRSCLPGNFTQNS Y S+L+LLF   + N   + 
Sbjct: 1   MLNFLTFLFLLCFLIHGNAQTRVSYPYRSCLPGNFTQNSTYHSNLDLLFSNLSANGPPKN 60

Query: 71  TFHSTSVGLTSTDTVYGLFQCWEDVTDN-CGACVATAINDTANNFCRFRKGAVSWYDGCI 130
            F +TS G    D VYGLFQC  DV+D+ C + +ATAI D A  +C  RKGAV+WYD CI
Sbjct: 61  RFFNTSAGRPPNDAVYGLFQCRGDVSDSVCRSFLATAIKDVAQEYCPLRKGAVAWYDECI 120

Query: 131 LRYSNQSIFSDLSTVPNLMLLNTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMVAT 190
            RYS+Q  FSD+ST P+L LLNTA+ID DK RFNQLVMS    T ARAA+A SV E+ AT
Sbjct: 121 FRYSDQRFFSDVSTEPSLPLLNTAEIDVDKARFNQLVMSMLRATAARAANA-SVGELFAT 180

Query: 191 REANFTFH-TLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYEI 250
           +EANFT    LYTLAQCTGDLS ++C  CLRQAI  +PSCC+NKRGGRVL+PSCYVRYE+
Sbjct: 181 QEANFTSDLPLYTLAQCTGDLSNTDCELCLRQAINGIPSCCSNKRGGRVLFPSCYVRYEV 240

Query: 251 YPFYRLTANNPPPIPPSTP-----KTPPLPPPPHAPSGETRSSTLIILVTVLPITVSILL 310
           Y FY  T  N  P P +TP      TPPLPPPP    G+ R ST++I+  V PIT+SILL
Sbjct: 241 YMFYEQTPTNSVPPPATTPPNNTTPTPPLPPPP--TPGKRRISTMLIVAIVAPITISILL 300

Query: 311 FV-GWCFLRQRAKKRYTTVKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGGF 370
           FV G CFLRQR +KR++ VKED SVV+ ++T+ESLQFDF TIDAATNKFS EN++GEGGF
Sbjct: 301 FVLGCCFLRQRVRKRHSAVKED-SVVNEMTTMESLQFDFKTIDAATNKFSEENKLGEGGF 360

Query: 371 GAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNET-------------------------- 430
           G V+KGRLENG+EIAVKRLSRGSLQGSEEFKNE                           
Sbjct: 361 GVVFKGRLENGEEIAVKRLSRGSLQGSEEFKNEVMLVAQLQHRNLVRLLGFCLEGEEKIL 420

Query: 431 -----PKR-----------QKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNV 490
                P +           Q +LDWL RYKIINGI RG+LYLHEDSRLRIIHR LKASN+
Sbjct: 421 IYEYIPNKSLDFFLFDLEGQTQLDWLKRYKIINGIARGMLYLHEDSRLRIIHRDLKASNI 480

Query: 491 LLDENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVL 550
           LLDE MN K+SDFGMARIIQVD+++ NT RI   YGYMSPEYAMHG F  KSDVYSFGVL
Sbjct: 481 LLDEYMNAKISDFGMARIIQVDESQVNTKRIVGTYGYMSPEYAMHGIFSMKSDVYSFGVL 540

Query: 551 VLEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIAL 610
           VLEI+S +KN++FYLS L++D+LTY WKLWK E PLE+LDP L ++Y RNEVLRCIHIAL
Sbjct: 541 VLEILSGQKNSSFYLSDLAEDLLTYAWKLWKTEQPLEILDPVLRNSYSRNEVLRCIHIAL 600

Query: 611 LCVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIR--NTD-NNSNQSASRSVQWSAN 621
           LCVQED S RPSMASIVLMLNS S  LPLP EPA F+R  NTD  +S+ S  R VQWS N
Sbjct: 601 LCVQEDSSQRPSMASIVLMLNSNSVTLPLPEEPAFFMRSKNTDIKDSDISTDRFVQWSVN 660

BLAST of Moc03g28140.1 vs. ExPASy TrEMBL
Match: A0A6J1KPY4 (cysteine-rich receptor-like protein kinase 10 OS=Cucurbita maxima OX=3661 GN=LOC111495427 PE=4 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 6.8e-212
Identity = 415/669 (62.03%), Postives = 486/669 (72.65%), Query Frame = 0

Query: 11  ILNFLSFIFLPSFI---TAQTLLQYPYRSCLPGNFTQNSAYQSSLNLLFLEFN-NVAHQK 70
           +LNF++F+FL  F+    AQT + YPYRSCLPGNFTQNS Y S+LNLLF   + N     
Sbjct: 1   MLNFVTFLFLLCFLIHGNAQTPVPYPYRSCLPGNFTQNSTYHSNLNLLFSNLSANAPPNN 60

Query: 71  TFHSTSVGLTSTDTVYGLFQCWEDVTDN-CGACVATAINDTANNFCRFRKGAVSWYDGCI 130
            F +TS G    D VYGLFQC  DV+D+ C + +ATAI D A  +C  R GAV+WYD CI
Sbjct: 61  RFFNTSAGRPPNDAVYGLFQCRGDVSDSVCRSFLATAIKDVAEKYCPLRNGAVAWYDECI 120

Query: 131 LRYSNQSIFSDLSTVPNLMLLNTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMVAT 190
            RYS+Q  FSD+ST P+L LLNT +ID DK RFNQLVMST   T ARAA+A SV E+ AT
Sbjct: 121 FRYSDQPFFSDVSTDPSLSLLNTDEIDVDKARFNQLVMSTLRATAARAANA-SVGELFAT 180

Query: 191 REANFTFH-TLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYEI 250
           +EANFT    L+TLAQCTGDLS ++C  CLRQAI  +PSCC+NKRGGRVL+PSCYVRYE+
Sbjct: 181 QEANFTSDLPLHTLAQCTGDLSNTDCELCLRQAINGIPSCCSNKRGGRVLFPSCYVRYEV 240

Query: 251 YPFYRLTANNPPPIPPSTP-----KTPPLPPPPHAPSGETRSSTLIILVTVLPITVSILL 310
           Y FY  T  N  P P +TP      TPPLPPPP  P G+ R ST++I+  V PIT+SILL
Sbjct: 241 YMFYEQTLTNSVPPPATTPPNNTTPTPPLPPPPPTP-GKRRISTMLIVAIVAPITISILL 300

Query: 311 FV-GWCFLRQRAKKRYTTVKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGGF 370
           FV G CFLRQRA+KR++TVKED SVV+ ++TVESL FDF TID+ATNKFS EN++GEGGF
Sbjct: 301 FVLGCCFLRQRARKRHSTVKED-SVVNEMTTVESLLFDFKTIDSATNKFSEENKLGEGGF 360

Query: 371 GAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNET-------------------------- 430
           G V+KGR ENG+EIAVKRLSRGSLQGSEEFKNE                           
Sbjct: 361 GVVFKGRFENGEEIAVKRLSRGSLQGSEEFKNEVLLVAQLQHRNLVRLLGFCLEGEEKIL 420

Query: 431 ----------------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNV 490
                            +RQ  LDWL RYKIINGI RG+LYLHEDSRLRIIHR LKASN+
Sbjct: 421 IYEYIPNKSLDFFLFDSERQTRLDWLKRYKIINGIARGMLYLHEDSRLRIIHRDLKASNI 480

Query: 491 LLDENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVL 550
           LLDE MN K+SDFGMARIIQVD+++ NT RI   YGYMSPEYAMHG F  KSDVYSFGVL
Sbjct: 481 LLDEYMNAKISDFGMARIIQVDESQVNTKRIVGTYGYMSPEYAMHGIFSMKSDVYSFGVL 540

Query: 551 VLEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIAL 610
           VLEI+S +KN++FYLS L++D+LTY WKLWK E PLE+L P L + Y RNEVL+CIHIAL
Sbjct: 541 VLEILSGQKNSSFYLSDLAEDLLTYAWKLWKTEQPLEILHPVLRNCYSRNEVLKCIHIAL 600

Query: 611 LCVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIR--NTD-NNSNQSASRSVQWSAN 621
           LCVQEDPS RPSM SIVLML+S S  +PLP EPA F+R  NTD  NS+ S ++ VQWS N
Sbjct: 601 LCVQEDPSQRPSMTSIVLMLSSNSVTMPLPEEPAFFLRSKNTDIKNSDISTNKFVQWSVN 660

BLAST of Moc03g28140.1 vs. ExPASy TrEMBL
Match: A0A5A7UQH5 (Cysteine-rich receptor-like protein kinase 10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G00560 PE=4 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 1.1e-204
Identity = 407/655 (62.14%), Postives = 474/655 (72.37%), Query Frame = 0

Query: 11   ILNFLSFIFLPSFIT---AQTLLQYPYRSCLPGNFTQNSAYQSSLNLLFLEFN-NVAHQK 70
            +LN L+FIFL + +T   AQ    YPYR CL GNFTQNS YQ++LNLLF   + N     
Sbjct: 620  MLNLLTFIFLLNLLTHGNAQNEASYPYRRCLTGNFTQNSTYQANLNLLFSTLSTNGPPTN 679

Query: 71   TFHSTSVGLTSTDTVYGLFQCWEDVTD-NCGACVATAINDTANNFCRFRKGAVSWYDGCI 130
             F +TSVG +  DTVYGLFQC  DVT+  C   +ATA  D    +C   KGAV WYD  I
Sbjct: 680  RFFNTSVGRSPNDTVYGLFQCRGDVTNATCRTFLATATRDAERLYCPLAKGAVIWYDEII 739

Query: 131  LRYSNQSIFSDLSTVPNLMLLNTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMVAT 190
             RYS+Q  FS +++ PNL LLN  +ID DK RFNQLVMST   T A A  ASS+ E+ AT
Sbjct: 740  FRYSDQPFFSIITSTPNLRLLNLVNIDGDKARFNQLVMSTLRATAALA--ASSMDELFAT 799

Query: 191  REANFT-FHTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYEI 250
            + ANFT    +YTLAQCTGDLS ++C ECLRQA   +P+CC +K+GGRVL PSC VRYE+
Sbjct: 800  QMANFTQDRNIYTLAQCTGDLSNTDCGECLRQATNEIPNCCTDKQGGRVLLPSCIVRYEV 859

Query: 251  YPFYRLTANNPPPIPPSTPKTPPLPP--PPHAPSGETRSSTLIILVTVLPITVSILLFV- 310
            Y FY  +   PPP  P+   T P  P  P   P GE RSST++I+  V PITVSILLFV 
Sbjct: 860  YSFYETSI--PPPATPTPISTIPSRPLLPSSPPPGERRSSTVLIVAIVAPITVSILLFVM 919

Query: 311  GWCFLRQRAKKRYTTVKED--------HSVVSGISTVESLQFDFNTIDAATNKFSAENRI 370
            G CFLRQRAKKR + VKED        H++V  ++T +SLQFDF TI+AATNKFS ENR+
Sbjct: 920  GCCFLRQRAKKRDSAVKEDSGTFLLFNHNIVDEMTTSDSLQFDFKTIEAATNKFSEENRL 979

Query: 371  GEGGFGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNET--------------------- 430
            GEGGFGAV+KGRL+NGQEIAVKRLSRGSLQGSEEFKNE                      
Sbjct: 980  GEGGFGAVFKGRLDNGQEIAVKRLSRGSLQGSEEFKNEVMLVAKLQHRNLVRLLGFCLED 1039

Query: 431  PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNVLLDENMNPKVSDFGMA 490
             + QK+LDWL RYKIINGI RGILYLHEDSRLRIIHR LKASN+LLD +MN K+SDFGMA
Sbjct: 1040 QEGQKQLDWLKRYKIINGIARGILYLHEDSRLRIIHRDLKASNILLDGDMNAKISDFGMA 1099

Query: 491  RIIQVDQNEAN-TNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVLVLEIISSKKNNTFYL 550
            RIIQ+DQ++ N T+RI   YGYMSPEYAMHGNF  KSD+YSFGVLVLEIIS +KNNTFYL
Sbjct: 1100 RIIQMDQSQGNYTSRIVGTYGYMSPEYAMHGNFSMKSDIYSFGVLVLEIISGRKNNTFYL 1159

Query: 551  SGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIALLCVQEDPSCRPSMAS 610
            S L++DILTY W LWKD IPLELLDP L DNY RNEVLRCIHIALLCVQEDP+ RPSMAS
Sbjct: 1160 SDLAEDILTYAWALWKDGIPLELLDPMLKDNYSRNEVLRCIHIALLCVQEDPNSRPSMAS 1219

Query: 611  IVLMLNSYSFPLPLPNEPASFIRNTDN----NSNQSASRSVQWSANKISIIELDP 621
            IVLMLNSYS  LP+P EPA F+R+ DN     S+ S+++S + S N+ S+ EL P
Sbjct: 1220 IVLMLNSYSVTLPIPKEPALFVRSKDNGNTIGSDHSSNKSTKCSVNETSLSELHP 1270

BLAST of Moc03g28140.1 vs. ExPASy TrEMBL
Match: A0A5D3BHV8 (Cysteine-rich receptor-like protein kinase 10 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006080 PE=4 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 1.2e-203
Identity = 407/655 (62.14%), Postives = 473/655 (72.21%), Query Frame = 0

Query: 11   ILNFLSFIFLPSFIT---AQTLLQYPYRSCLPGNFTQNSAYQSSLNLLFLEFN-NVAHQK 70
            +LN L+FIFL + +T   AQ    YPYR CL GNFTQNS YQ++LNLLF   + N     
Sbjct: 652  MLNLLTFIFLLNLLTHGNAQNEASYPYRRCLTGNFTQNSTYQANLNLLFSTLSTNGPPTN 711

Query: 71   TFHSTSVGLTSTDTVYGLFQCWEDVTD-NCGACVATAINDTANNFCRFRKGAVSWYDGCI 130
             F +TSVG +  DTVYGLFQC  DVT+  C   +ATA  D    +C   KGAV WYD  I
Sbjct: 712  RFFNTSVGRSPNDTVYGLFQCRGDVTNATCRTFLATATRDAERLYCPLAKGAVIWYDEII 771

Query: 131  LRYSNQSIFSDLSTVPNLMLLNTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMVAT 190
             RYS+Q  FS +++ PNL LLN  +ID DK RFNQLVMST   T A A  ASS+ E+ AT
Sbjct: 772  FRYSDQPFFSIITSTPNLRLLNLVNIDGDKARFNQLVMSTLRATAALA--ASSMDELFAT 831

Query: 191  REANFT-FHTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYEI 250
            + ANFT    +YTLAQCTGDLS ++C ECLRQA   +P+CC +K+GGRVL PSC VRYE+
Sbjct: 832  QMANFTQDRNIYTLAQCTGDLSNTDCGECLRQATNEIPNCCTDKQGGRVLLPSCIVRYEV 891

Query: 251  YPFYRLTANNPPPIPPSTPKTPPLPP--PPHAPSGETRSSTLIILVTVLPITVSILLFV- 310
            Y FY  +   PPP  P+   T P  P  P   P GE RSST++I+  V PITVSILLFV 
Sbjct: 892  YSFYETSI--PPPATPTPISTIPSRPLLPSSPPPGERRSSTVLIVAIVAPITVSILLFVM 951

Query: 311  GWCFLRQRAKKRYTTVKEDH--------SVVSGISTVESLQFDFNTIDAATNKFSAENRI 370
            G CFLRQRAKKR + VKED         +VV  ++T +SLQFDF TI+AATNKFS ENR+
Sbjct: 952  GCCFLRQRAKKRDSAVKEDTKFGYDLFCAVVDEMTTSDSLQFDFKTIEAATNKFSEENRL 1011

Query: 371  GEGGFGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNET--------------------- 430
            GEGGFGAV+KGRL+NGQEIAVKRLSRGSLQGSEEFKNE                      
Sbjct: 1012 GEGGFGAVFKGRLDNGQEIAVKRLSRGSLQGSEEFKNEVMLVAKLQHRNLVRLLGFCLED 1071

Query: 431  PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNVLLDENMNPKVSDFGMA 490
             + QK+LDWL RYKIINGI RGILYLHEDSRLRIIHR LKASN+LLD +MN K+SDFGMA
Sbjct: 1072 QEGQKQLDWLKRYKIINGIARGILYLHEDSRLRIIHRDLKASNILLDGDMNAKISDFGMA 1131

Query: 491  RIIQVDQNEAN-TNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVLVLEIISSKKNNTFYL 550
            RIIQ+DQ++ N T+RI   YGYMSPEYAMHGNF  KSD+YSFGVLVLEIIS +KNNTFYL
Sbjct: 1132 RIIQMDQSQGNYTSRIVGTYGYMSPEYAMHGNFSMKSDIYSFGVLVLEIISGRKNNTFYL 1191

Query: 551  SGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIALLCVQEDPSCRPSMAS 610
            S L++DILTY W LWKD IPLELLDP L DNY RNEVLRCIHIALLCVQEDP+ RPSMAS
Sbjct: 1192 SDLAEDILTYAWALWKDGIPLELLDPMLKDNYSRNEVLRCIHIALLCVQEDPNSRPSMAS 1251

Query: 611  IVLMLNSYSFPLPLPNEPASFIRNTDN----NSNQSASRSVQWSANKISIIELDP 621
            IVLMLNSYS  LP+P EPA F+R+ DN     S+ S+++S + S N+ S+ EL P
Sbjct: 1252 IVLMLNSYSVTLPIPKEPALFVRSKDNGNTIGSDHSSNKSTKCSVNETSLSELHP 1302

BLAST of Moc03g28140.1 vs. TAIR 10
Match: AT4G23180.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 10 )

HSP 1 Score: 487.6 bits (1254), Expect = 1.4e-137
Identity = 295/676 (43.64%), Postives = 410/676 (60.65%), Query Frame = 0

Query: 5   KSVVLGILNFLSFIFLPSFITAQTLL----QYPYRSCL-PGNFTQNSAYQSSLNLLF--L 64
           +S ++   +   F+FL SF+T+  +      Y Y +C    N+T NS Y ++L  L   L
Sbjct: 8   ESPIMSYYSSFFFLFLFSFLTSFRVSAQDPTYVYHTCQNTANYTSNSTYNNNLKTLLASL 67

Query: 65  EFNNVAHQKTFHSTSVGLTSTDTVYGLFQCWEDV-TDNCGACVATAINDTANNFCRFRKG 124
              N ++   F + +VG  + D V GLF C  DV T+ C  CV+ A+NDT    C  +K 
Sbjct: 68  SSRNASYSTGFQNATVG-QAPDRVTGLFNCRGDVSTEVCRRCVSFAVNDTLTR-CPNQKE 127

Query: 125 AVSWYDGCILRYSNQSIFSDLSTVPNLMLLNTADIDADK-PRFNQLVMSTFGGTVARAAD 184
           A  +YD C+LRYSNQ+I S L T   ++L+NT ++ +++    + LV+ T       A +
Sbjct: 128 ATLYYDECVLRYSNQNILSTLITTGGVILVNTRNVTSNQLDLLSDLVLPTLNQAATVALN 187

Query: 185 ASSVAEMVATREANFT-FHTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVL 244
           +S   +   TR+ NFT   + Y L QCT DL+  +C  CL+  I  +P+   ++ G R++
Sbjct: 188 SS---KKFGTRKNNFTALQSFYGLVQCTPDLTRQDCSRCLQLVINQIPT---DRIGARII 247

Query: 245 YPSCYVRYEIYPFYRLTANNPPPIPPSTPKTPPLPPPPHAPSGETRSSTLIILVTVLPIT 304
            PSC  RYEIY FY  +A  PPP PPS   TPP+  PP   SG+  +S ++++  V+PI 
Sbjct: 248 NPSCTSRYEIYAFYTESAVPPPPPPPSI-STPPVSAPPR--SGKDGNSKVLVIAIVVPII 307

Query: 305 VSILLFV-GWCFLRQRAKKRYTTVKEDHSVVSG--ISTVESLQFDFNTIDAATNKFSAEN 364
           V++LLF+ G+CFL +RA+K Y T     S  +G  I+T +SLQ D+ TI  AT+ F   N
Sbjct: 308 VAVLLFIAGYCFLTRRARKSYYT----PSAFAGDDITTADSLQLDYRTIQTATDDFVESN 367

Query: 365 RIGEGGFGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNET------------------- 424
           +IG+GGFG VYKG L +G E+AVKRLS+ S QG  EFKNE                    
Sbjct: 368 KIGQGGFGEVYKGTLSDGTEVAVKRLSKSSGQGEVEFKNEVVLVAKLQHRNLVRLLGFCL 427

Query: 425 -----------------------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHR 484
                                  P ++ +LDW  RYKII G+ RGILYLH+DSRL IIHR
Sbjct: 428 DGEERVLVYEYVPNKSLDYFLFDPAKKGQLDWTRRYKIIGGVARGILYLHQDSRLTIIHR 487

Query: 485 GLKASNVLLDENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSD 544
            LKASN+LLD +MNPK++DFGMARI  +DQ E NT+RI   YGYMSPEYAMHG +  KSD
Sbjct: 488 DLKASNILLDADMNPKIADFGMARIFGLDQTEENTSRIVGTYGYMSPEYAMHGQYSMKSD 547

Query: 545 VYSFGVLVLEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVL 604
           VYSFGVLVLEIIS KKN++FY +  + D+++Y W LW +  PLEL+DP++ +N  RNEV+
Sbjct: 548 VYSFGVLVLEIISGKKNSSFYQTDGAHDLVSYAWGLWSNGRPLELVDPAIVENCQRNEVV 607

Query: 605 RCIHIALLCVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIRN---TDNNSNQSASR 621
           RC+HI LLCVQEDP+ RP++++IVLML S +  LP+P +P  F ++    D     + S+
Sbjct: 608 RCVHIGLLCVQEDPAERPTLSTIVLMLTSNTVTLPVPRQPGLFFQSRIGKDPLDTDTTSK 667

BLAST of Moc03g28140.1 vs. TAIR 10
Match: AT4G05200.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 25 )

HSP 1 Score: 472.6 bits (1215), Expect = 4.8e-133
Identity = 278/627 (44.34%), Postives = 375/627 (59.81%), Query Frame = 0

Query: 42  FTQNSAYQSSLN--LLFLEFNNVAHQKTFHSTSVG-LTSTDTVYGLFQCWEDVT-DNCGA 101
           +++NS+Y ++L   L  L   N A+   F + + G    ++ VYG+F C  DV+ + C  
Sbjct: 40  YSRNSSYLTNLRTVLSSLSSPNAAYASLFDNAAAGEENDSNRVYGVFLCRGDVSAEICRD 99

Query: 102 CVATAINDTANNFCRFRKGAVSWYDGCILRYSNQSIFSDLSTVPNLMLLNTADIDADK-P 161
           CVA A N+T    C   K AV WYD C++RYSNQSI   +   P + L N  +I  ++  
Sbjct: 100 CVAFAANETLQR-CPREKVAVIWYDECMVRYSNQSIVGQMRIRPGVFLTNKQNITENQVS 159

Query: 162 RFNQLVMSTFGGTVARAADASSVAEMVATREANFT-FHTLYTLAQCTGDLSISNCRECLR 221
           RFN+ + +       +AA +S      AT +ANFT F T+Y+L QCT DL+  +C  CLR
Sbjct: 160 RFNESLPALLIDVAVKAALSS---RKFATEKANFTVFQTIYSLVQCTPDLTNQDCESCLR 219

Query: 222 QAIVAVPSCCANKRGGRVLYPSCYVRYEIYPFYRLTANNPPPIPP--STPKTPPLPPPPH 281
           Q I  +P CC    GGRV+ PSC  RYE+YPFY  T    P  PP  ST   PPL  P  
Sbjct: 220 QVINYLPRCCDRSVGGRVIAPSCSFRYELYPFYNETIAAAPMAPPPSSTVTAPPLNIP-- 279

Query: 282 APSGETRSSTLIILVTVLPITVSILLFVGWCFLRQRAKKRYTTVKEDHSVVSGISTVESL 341
           +  G+ ++ T+I+    +P++V +LL    C+L  R +    + + +     GI++ E+L
Sbjct: 280 SEKGKGKNLTVIVTAIAVPVSVCVLLLGAMCWLLARRRNNKLSAETEDLDEDGITSTETL 339

Query: 342 QFDFNTIDAATNKFSAENRIGEGGFGAVYKGRLENGQEIAVKRLSRGSLQGSEEFKNETP 401
           QF F+ I+AATNKFS  N++G GGFG VYKG+L  G+ +A+KRLS+GS QG+EEFKNE  
Sbjct: 340 QFQFSAIEAATNKFSESNKLGHGGFGEVYKGQLITGETVAIKRLSQGSTQGAEEFKNEVD 399

Query: 402 ------------------------------------------KRQKELDWLTRYKIINGI 461
                                                     ++++ LDW  RYKII GI
Sbjct: 400 VVAKLQHRNLAKLLGYCLDGEEKILVYEFVPNKSLDYFLFDNEKRRVLDWQRRYKIIEGI 459

Query: 462 VRGILYLHEDSRLRIIHRGLKASNVLLDENMNPKVSDFGMARIIQVDQNEANTNRI--AY 521
            RGILYLH DSRL IIHR LKASN+LLD +M+PK+SDFGMARI  VDQ +ANT RI   Y
Sbjct: 460 ARGILYLHRDSRLTIIHRDLKASNILLDADMHPKISDFGMARIFGVDQTQANTKRIVGTY 519

Query: 522 GYMSPEYAMHGNFFTKSDVYSFGVLVLEIISSKKNNTFYLSGLSKDILTYTWKLWKDEIP 581
           GYMSPEYA+HG +  KSDVYSFGVLVLE+I+ KKN++FY      D++TY WKLW +  P
Sbjct: 520 GYMSPEYAIHGKYSVKSDVYSFGVLVLELITGKKNSSFYEEDGLGDLVTYVWKLWVENSP 579

Query: 582 LELLDPSLGDNYLRNEVLRCIHIALLCVQEDPSCRPSMASIVLMLNSYSFPLPLPNEPAS 616
           LEL+D ++  N+  NEV+RCIHIALLCVQED S RPSM  I++M+NS++  LP+P     
Sbjct: 580 LELVDEAMRGNFQTNEVIRCIHIALLCVQEDSSERPSMDDILVMMNSFTVTLPIPKRSGF 639

BLAST of Moc03g28140.1 vs. TAIR 10
Match: AT4G23130.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 5 )

HSP 1 Score: 450.7 bits (1158), Expect = 1.9e-126
Identity = 288/664 (43.37%), Postives = 386/664 (58.13%), Query Frame = 0

Query: 15  LSFIFLPSF----ITAQTLLQYP--YRSCLPGNFTQNSAYQSSLNLLF--LEFNNVAHQK 74
           L+F+FL +F    +     LQ P           ++NS Y S+L  L   L  NN     
Sbjct: 7   LNFLFLLTFFIGSLRVSAQLQDPTYVGHVCTNRISRNSIYFSNLQTLLTSLSSNNAYFSL 66

Query: 75  TFHSTSVGLTSTDTVYGLFQCWEDVT-DNCGACVATAINDTANNFCRFRKGAVSWYDGCI 134
             HS + G  S D V+GL+ C  D++ ++C  CV  A  DT +  C   K  +  YD C+
Sbjct: 67  GSHSLTKGQNS-DMVFGLYLCKGDLSPESCRECVIFAAKDTRSR-CPGGKEFLIQYDECM 126

Query: 135 LRYSNQSIFSDLSTVPNLMLLNTADIDADK-PRFNQLVMSTFGGTVARAADASSVAEMVA 194
           L YS+++IF D  T   ++  NT  + AD+  RFN  V+S    +   AA+++S  +  A
Sbjct: 127 LGYSDRNIFMDTVTTTTIITWNTQKVTADQSDRFNDAVLSLMKKSAEEAANSTS--KKFA 186

Query: 195 TREANF-TFHTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYE 254
            ++++F +  +LY   QC  DL+  +C  CL+Q+I        NK GGR L PSC  RYE
Sbjct: 187 VKKSDFSSSQSLYASVQCIPDLTSEDCVMCLQQSI---KELYFNKVGGRFLVPSCNSRYE 246

Query: 255 IYPFYRLTANNPPPIPPSTPKTPPLPPPPHAPSGETRSSTLIILVTVLPITVSILLFVG- 314
           +YPFY+ T       PP +    PL   P  P G+ ++ST+II+  V+P+ +S+L+ V  
Sbjct: 247 VYPFYKETIEGTVLPPPVSAPPLPLVSTPSFPPGKGKNSTVIIIAIVVPVAISVLICVAV 306

Query: 315 WCF-LRQRAKKRYTTVKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGGFGAV 374
           + F   +RAKK Y T +ED      I+T  SLQFDF  I+AAT+KFS  N++G+GGFG V
Sbjct: 307 FSFHASKRAKKTYDTPEED-----DITTAGSLQFDFKVIEAATDKFSMCNKLGQGGFGQV 366

Query: 375 YKGRLENGQEIAVKRLSRGSLQGSEEFKNET----------------------------- 434
           YKG L NG ++AVKRLS+ S QG +EFKNE                              
Sbjct: 367 YKGTLPNGVQVAVKRLSKTSGQGEKEFKNEVVVVAKLQHRNLVKLLGFCLEREEKILVYE 426

Query: 435 -------------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNVLLD 494
                         + Q +LDW TRYKII GI RGILYLH+DSRL IIHR LKA N+LLD
Sbjct: 427 FVSNKSLDYFLFDSRMQSQLDWTTRYKIIGGIARGILYLHQDSRLTIIHRDLKAGNILLD 486

Query: 495 ENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVLVLE 554
            +MNPKV+DFGMARI ++DQ EA+T R+   YGYMSPEYAM+G F  KSDVYSFGVLVLE
Sbjct: 487 ADMNPKVADFGMARIFEIDQTEAHTRRVVGTYGYMSPEYAMYGQFSMKSDVYSFGVLVLE 546

Query: 555 IISSKKNNTFYLSGLS-KDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIALLC 614
           IIS +KN++ Y    S  +++TYTW+LW D  PL+L+D S  D+Y RNE++RCIHIALLC
Sbjct: 547 IISGRKNSSLYQMDASFGNLVTYTWRLWSDGSPLDLVDSSFRDSYQRNEIIRCIHIALLC 606

Query: 615 VQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIRNTDNNSNQSASRSVQWSANKISII 621
           VQED   RP+M++IV ML + S  L +P  P  F R+    +  S  +S   S +  SI 
Sbjct: 607 VQEDTENRPTMSAIVQMLTTSSIALAVPQPPGFFFRSNHEQAGPSMDKSSLCSIDAASIT 658

BLAST of Moc03g28140.1 vs. TAIR 10
Match: AT4G23130.2 (cysteine-rich RLK (RECEPTOR-like protein kinase) 5 )

HSP 1 Score: 445.7 bits (1145), Expect = 6.3e-125
Identity = 286/664 (43.07%), Postives = 384/664 (57.83%), Query Frame = 0

Query: 15  LSFIFLPSF----ITAQTLLQYP--YRSCLPGNFTQNSAYQSSLNLLF--LEFNNVAHQK 74
           L+F+FL +F    +     LQ P           ++NS Y S+L  L   L  NN     
Sbjct: 7   LNFLFLLTFFIGSLRVSAQLQDPTYVGHVCTNRISRNSIYFSNLQTLLTSLSSNNAYFSL 66

Query: 75  TFHSTSVGLTSTDTVYGLFQCWEDVT-DNCGACVATAINDTANNFCRFRKGAVSWYDGCI 134
             HS + G  S D V+GL+ C  D++ ++C  CV  A  DT +  C   K  +  YD C+
Sbjct: 67  GSHSLTKGQNS-DMVFGLYLCKGDLSPESCRECVIFAAKDTRSR-CPGGKEFLIQYDECM 126

Query: 135 LRYSNQSIFSDLSTVPNLMLLNTADIDADK-PRFNQLVMSTFGGTVARAADASSVAEMVA 194
           L YS+++IF D  T   ++  NT  + AD+  RFN  V+S    +   AA+++S  +  A
Sbjct: 127 LGYSDRNIFMDTVTTTTIITWNTQKVTADQSDRFNDAVLSLMKKSAEEAANSTS--KKFA 186

Query: 195 TREANF-TFHTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYE 254
            ++++F +  +LY   QC  DL+  +C  CL+Q+I        NK GGR L PSC  RYE
Sbjct: 187 VKKSDFSSSQSLYASVQCIPDLTSEDCVMCLQQSI---KELYFNKVGGRFLVPSCNSRYE 246

Query: 255 IYPFYRLTANNPPPIPPSTPKTPPLPPPPHAPSGETRSSTLIILVTVLPITVSILLFVG- 314
           +YPFY+ T       PP +    PL   P  P G+ ++ST+II+  V+P+ +S+L+ V  
Sbjct: 247 VYPFYKETIEGTVLPPPVSAPPLPLVSTPSFPPGKGKNSTVIIIAIVVPVAISVLICVAV 306

Query: 315 WCF-LRQRAKKRYTTVKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGGFGAV 374
           + F   +RAKK Y T   +      I+T  SLQFDF  I+AAT+KFS  N++G+GGFG V
Sbjct: 307 FSFHASKRAKKTYDTPGANDE-EDDITTAGSLQFDFKVIEAATDKFSMCNKLGQGGFGQV 366

Query: 375 YKGRLENGQEIAVKRLSRGSLQGSEEFKNET----------------------------- 434
           YKG L NG ++AVKRLS+ S QG +EFKNE                              
Sbjct: 367 YKGTLPNGVQVAVKRLSKTSGQGEKEFKNEVVVVAKLQHRNLVKLLGFCLEREEKILVYE 426

Query: 435 -------------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNVLLD 494
                         + Q +LDW TRYKII GI RGILYLH+DSRL IIHR LKA N+LLD
Sbjct: 427 FVSNKSLDYFLFDSRMQSQLDWTTRYKIIGGIARGILYLHQDSRLTIIHRDLKAGNILLD 486

Query: 495 ENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVLVLE 554
            +MNPKV+DFGMARI ++DQ EA+T R+   YGYMSPEYAM+G F  KSDVYSFGVLVLE
Sbjct: 487 ADMNPKVADFGMARIFEIDQTEAHTRRVVGTYGYMSPEYAMYGQFSMKSDVYSFGVLVLE 546

Query: 555 IISSKKNNTFYLSGLS-KDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIALLC 614
           IIS +KN++ Y    S  +++TYTW+LW D  PL+L+D S  D+Y RNE++RCIHIALLC
Sbjct: 547 IISGRKNSSLYQMDASFGNLVTYTWRLWSDGSPLDLVDSSFRDSYQRNEIIRCIHIALLC 606

Query: 615 VQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIRNTDNNSNQSASRSVQWSANKISII 621
           VQED   RP+M++IV ML + S  L +P  P  F R+    +  S  +S   S +  SI 
Sbjct: 607 VQEDTENRPTMSAIVQMLTTSSIALAVPQPPGFFFRSNHEQAGPSMDKSSLCSIDAASIT 662

BLAST of Moc03g28140.1 vs. TAIR 10
Match: AT4G23150.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 7 )

HSP 1 Score: 432.6 bits (1111), Expect = 5.5e-121
Identity = 277/669 (41.41%), Postives = 378/669 (56.50%), Query Frame = 0

Query: 17  FIFLPSFIT-----AQTLLQYPYRSCLPGNFTQNSAYQSSLNLLF--LEFNNVAHQKTFH 76
           F+FL SF+T     AQ      Y       ++ NS Y ++L  L   L   N ++   F 
Sbjct: 9   FLFLFSFLTSFRASAQDPRFLAYYCPNATTYSSNSTYLTNLKTLLSSLSSRNASYSTGFQ 68

Query: 77  STSVGLTSTDTVYGLFQCWEDVT-DNCGACVATAINDTANNFCRFRKGAVSWYDGCILRY 136
           + +VG  + D V GLF C  DV+ + C  CV  A+N+T +  C  ++ AV +Y+ CILRY
Sbjct: 69  NATVG-QALDRVTGLFLCRGDVSPEVCRNCVTFAVNNTFSR-CPNQREAVFYYEECILRY 128

Query: 137 SNQSIFSDLSTVPNLMLL----NTADIDADKPRFNQLVMSTFGGTVARAADASSVAEMVA 196
           S+++I S   T     +L    + + I     +F  LV+S        AAD       + 
Sbjct: 129 SHKNILSTAITNEGEFILRNPNHISPIQNQINQFTNLVLSNMNQIAIEAADNPRKFSTIK 188

Query: 197 TREANFTFHTLYTLAQCTGDLSISNCRECLRQAIVAVPSCCANKRGGRVLYPSCYVRYEI 256
           T        T Y L QCT DLS  NC  CL  +I  +P    ++ G R  +PSC  RYE+
Sbjct: 189 TELT--ALQTFYGLVQCTPDLSRQNCMNCLTSSINRMP---FSRIGARQFWPSCNSRYEL 248

Query: 257 YPFYRLTANNPPPIPPSTPKTPPLPPPPHAPSGETRSSTLIILVTVLPITVSILLFV-GW 316
           Y FY  TA   PP     P  PPL  P  + S ++ +S ++++  V+PI V++L+F+ G+
Sbjct: 249 YDFYNETAIGTPP-----PPLPPLASP--SLSDKSGNSNVVVVAVVVPIIVAVLIFIAGY 308

Query: 317 CFLRQRAKKRYTT---VKEDHSVVSGISTVESLQFDFNTIDAATNKFSAENRIGEGGFGA 376
           CF  +RAKK Y T   + ED       +T+ESLQ D+  I AATN FS  N+IG GGFG 
Sbjct: 309 CFFAKRAKKTYGTTPALDEDDK-----TTIESLQLDYRAIQAATNDFSENNKIGRGGFGD 368

Query: 377 VYKGRLENGQEIAVKRLSRGSLQGSEEFKNET---------------------------- 436
           VYKG   NG E+AVKRLS+ S QG  EFKNE                             
Sbjct: 369 VYKGTFSNGTEVAVKRLSKTSEQGDTEFKNEVVVVANLRHKNLVRILGFSIEREERILVY 428

Query: 437 --------------PKRQKELDWLTRYKIINGIVRGILYLHEDSRLRIIHRGLKASNVLL 496
                         P ++ +L W  RY II GI RGILYLH+DSRL IIHR LKASN+LL
Sbjct: 429 EYVENKSLDNFLFDPAKKGQLYWTQRYHIIGGIARGILYLHQDSRLTIIHRDLKASNILL 488

Query: 497 DENMNPKVSDFGMARIIQVDQNEANTNRI--AYGYMSPEYAMHGNFFTKSDVYSFGVLVL 556
           D +MNPK++DFGMARI  +DQ + NT+RI   YGYMSPEYAM G F  KSDVYSFGVLVL
Sbjct: 489 DADMNPKIADFGMARIFGMDQTQQNTSRIVGTYGYMSPEYAMRGQFSMKSDVYSFGVLVL 548

Query: 557 EIISSKKNNTFYLSGLSKDILTYTWKLWKDEIPLELLDPSLGDNYLRNEVLRCIHIALLC 616
           EIIS +KNN+F  +  ++D++T+ W+LW++   L+L+DP + D+  ++EV+RC HI LLC
Sbjct: 549 EIISGRKNNSFIETDDAQDLVTHAWRLWRNGTALDLVDPFIADSCRKSEVVRCTHIGLLC 608

Query: 617 VQEDPSCRPSMASIVLMLNSYSFPLPLPNEPASFIR-----NTDNNSNQSASRSVQWSAN 621
           VQEDP  RP+M++I +ML S +  LP P +P  F+R     N  ++   + ++SV  S +
Sbjct: 609 VQEDPVKRPAMSTISVMLTSNTMALPAPQQPGFFVRSRPGTNRLDSDQSTTNKSVTVSID 658

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022139941.11.8e-23066.62cysteine-rich receptor-like protein kinase 10 [Momordica charantia][more]
XP_038893612.11.3e-21263.02cysteine-rich receptor-like protein kinase 10 isoform X2 [Benincasa hispida][more]
XP_022933188.12.2e-21262.93cysteine-rich receptor-like protein kinase 10 [Cucurbita moschata][more]
XP_023001238.11.4e-21162.03cysteine-rich receptor-like protein kinase 10 [Cucurbita maxima][more]
XP_023519606.13.5e-21061.73cysteine-rich receptor-like protein kinase 10 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8GYA42.0e-13643.64Cysteine-rich receptor-like protein kinase 10 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9M0X56.7e-13244.34Cysteine-rich receptor-like protein kinase 25 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9C5S82.7e-12543.37Cysteine-rich receptor-like protein kinase 5 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8L7G37.7e-12041.41Cysteine-rich receptor-like protein kinase 7 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8W4G64.7e-11742.49Cysteine-rich receptor-like protein kinase 15 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1CE698.6e-23166.62cysteine-rich receptor-like protein kinase 10 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1F4171.1e-21262.93cysteine-rich receptor-like protein kinase 10 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1KPY46.8e-21262.03cysteine-rich receptor-like protein kinase 10 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5A7UQH51.1e-20462.14Cysteine-rich receptor-like protein kinase 10 OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3BHV81.2e-20362.14Cysteine-rich receptor-like protein kinase 10 OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
AT4G23180.11.4e-13743.64cysteine-rich RLK (RECEPTOR-like protein kinase) 10 [more]
AT4G05200.14.8e-13344.34cysteine-rich RLK (RECEPTOR-like protein kinase) 25 [more]
AT4G23130.11.9e-12643.37cysteine-rich RLK (RECEPTOR-like protein kinase) 5 [more]
AT4G23130.26.3e-12543.07cysteine-rich RLK (RECEPTOR-like protein kinase) 5 [more]
AT4G23150.15.5e-12141.41cysteine-rich RLK (RECEPTOR-like protein kinase) 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 396..572
e-value: 9.9E-24
score: 84.0
IPR038408Gnk2-homologous domain superfamilyGENE3D3.30.430.20coord: 30..132
e-value: 5.5E-19
score: 70.2
coord: 143..247
e-value: 7.2E-26
score: 92.4
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 323..392
e-value: 1.1E-19
score: 72.4
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 393..615
e-value: 4.2E-41
score: 142.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..276
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..277
NoneNo IPR availablePANTHERPTHR27002:SF544CYSTEINE-RICH RECEPTOR-KINASE-LIKE PROTEINcoord: 393..601
NoneNo IPR availablePANTHERPTHR27002:SF544CYSTEINE-RICH RECEPTOR-KINASE-LIKE PROTEINcoord: 27..391
NoneNo IPR availablePANTHERPTHR27002RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8coord: 393..601
coord: 27..391
IPR002902Gnk2-homologous domainPFAMPF01657Stress-antifungcoord: 163..244
e-value: 2.7E-12
score: 47.2
coord: 40..131
e-value: 2.9E-18
score: 66.3
IPR002902Gnk2-homologous domainPROSITEPS51473GNK2coord: 139..247
score: 20.019171
IPR002902Gnk2-homologous domainPROSITEPS51473GNK2coord: 30..133
score: 14.922837
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 353..375
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 347..580
score: 19.764292
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 332..607

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Moc03g28140Moc03g28140gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Moc03g28140.1.exon7Moc03g28140.1.exon7exon
Moc03g28140.1.exon6Moc03g28140.1.exon6exon
Moc03g28140.1.exon5Moc03g28140.1.exon5exon
Moc03g28140.1.exon4Moc03g28140.1.exon4exon
Moc03g28140.1.exon3Moc03g28140.1.exon3exon
Moc03g28140.1.exon2Moc03g28140.1.exon2exon
Moc03g28140.1.exon1Moc03g28140.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Moc03g28140.1cds.Moc03g28140.1_7CDS
cds.Moc03g28140.1cds.Moc03g28140.1_6CDS
cds.Moc03g28140.1cds.Moc03g28140.1_5CDS
cds.Moc03g28140.1cds.Moc03g28140.1_4CDS
cds.Moc03g28140.1cds.Moc03g28140.1_3CDS
cds.Moc03g28140.1cds.Moc03g28140.1_2CDS
cds.Moc03g28140.1cds.Moc03g28140.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Moc03g28140.1Moc03g28140.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016020 membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity