MS022954.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS022954.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	F-box/kelch-repeat protein
Location	scaffold357: 76756 .. 77802 (-)
Sequence length	1047
RNA-Seq Expression	MS022954.1
Synteny	MS022954.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGAAATTTTCAGCAACAAAAACAATGACTATGGCGGAATTGATTCCTGGTGTACCCGAAGAAATTGCCCTCGAGTGCTTAACTCGGTCCCACTACACGACTCACCGAGTCGCCGCCCGAGTCTGCCGCCGCTGGCGCCAGCTCTTCCTCGGCCCTGATTTCTACAAACTCCGTCACAAATCCGGCCGAACCCACAAGGCGGTCTCCTTAATTCAGTCACTTTCGGCGCAGGCCGGTCCCGACGGTGCCAAAGCCGCCACCTCCGCCAACGCATACGGCGTCTCCGTGTTCGATCCGGCCGCCGGAAATTGGACCCGAATCGAACCGATCGGAAAGTACCCGAACGGGCTGCCGCTGTTCTGCCGGATAATCAGCTCGAAGGGGAAATTGGTGGTGATCGGCGGGTGGGACCCGGCGACATACCGGCCGGTGGAGGACGTCTTCGTGTACGAGTTCGCGACGGAGAGGTGGAGGCAGGGGAAGGCGATGCCTGAGAGGAGGTCGTTCTTCGGCGCCGCCGCGCACGGCGGCGAAATCTTCGTGGCCGGTGGCCACGACGAGGGGAAGAACGCGGCGGCGACGGCGTGGGCGTACGACGTCAGACAGGACGAGTGGAGGGAGCTGCCGGCGATGGGGGCGGCGCGGGACGAGTGCGAGGCGGTGGCGATCGGATCGGAGATATGGGTGGTTAGCGGTTACGTGACGGAAAATCAAGGGGCGTTCGTCGATAGCGCTGAGGTTTTCGACACAAAGACGGGGACGTGGCGGCGCGTGGACGGCGCGTGGAAGGGGGAGAGGGGTCCGAGGGGGGTGGTGGGAGTGAGGGGAGGCGGAGGAATTTATAATTGGGCGGAGGCGGCGGCGGCGACGGCGAAGGAGGGGATGGTCGGAGTAGAATTGGGGGAAAGGGCGATGGTATTTGGGTGGGAGTATTTGGGAGGGAAGTATGGAATTTTTATGAGGGAAGGGCAAAATGGTAAATTCGAGAGGATGGAAGTTCCTTCGGAATTCTCGGGGTTTGTCCAGTCAGCTTGTTACACGGAGATT

mRNA sequence

GAGAAATTTTCAGCAACAAAAACAATGACTATGGCGGAATTGATTCCTGGTGTACCCGAAGAAATTGCCCTCGAGTGCTTAACTCGGTCCCACTACACGACTCACCGAGTCGCCGCCCGAGTCTGCCGCCGCTGGCGCCAGCTCTTCCTCGGCCCTGATTTCTACAAACTCCGTCACAAATCCGGCCGAACCCACAAGGCGGTCTCCTTAATTCAGTCACTTTCGGCGCAGGCCGGTCCCGACGGTGCCAAAGCCGCCACCTCCGCCAACGCATACGGCGTCTCCGTGTTCGATCCGGCCGCCGGAAATTGGACCCGAATCGAACCGATCGGAAAGTACCCGAACGGGCTGCCGCTGTTCTGCCGGATAATCAGCTCGAAGGGGAAATTGGTGGTGATCGGCGGGTGGGACCCGGCGACATACCGGCCGGTGGAGGACGTCTTCGTGTACGAGTTCGCGACGGAGAGGTGGAGGCAGGGGAAGGCGATGCCTGAGAGGAGGTCGTTCTTCGGCGCCGCCGCGCACGGCGGCGAAATCTTCGTGGCCGGTGGCCACGACGAGGGGAAGAACGCGGCGGCGACGGCGTGGGCGTACGACGTCAGACAGGACGAGTGGAGGGAGCTGCCGGCGATGGGGGCGGCGCGGGACGAGTGCGAGGCGGTGGCGATCGGATCGGAGATATGGGTGGTTAGCGGTTACGTGACGGAAAATCAAGGGGCGTTCGTCGATAGCGCTGAGGTTTTCGACACAAAGACGGGGACGTGGCGGCGCGTGGACGGCGCGTGGAAGGGGGAGAGGGGTCCGAGGGGGGTGGTGGGAGTGAGGGGAGGCGGAGGAATTTATAATTGGGCGGAGGCGGCGGCGGCGACGGCGAAGGAGGGGATGGTCGGAGTAGAATTGGGGGAAAGGGCGATGGTATTTGGGTGGGAGTATTTGGGAGGGAAGTATGGAATTTTTATGAGGGAAGGGCAAAATGGTAAATTCGAGAGGATGGAAGTTCCTTCGGAATTCTCGGGGTTTGTCCAGTCAGCTTGTTACACGGAGATT

Coding sequence (CDS)

GAGAAATTTTCAGCAACAAAAACAATGACTATGGCGGAATTGATTCCTGGTGTACCCGAAGAAATTGCCCTCGAGTGCTTAACTCGGTCCCACTACACGACTCACCGAGTCGCCGCCCGAGTCTGCCGCCGCTGGCGCCAGCTCTTCCTCGGCCCTGATTTCTACAAACTCCGTCACAAATCCGGCCGAACCCACAAGGCGGTCTCCTTAATTCAGTCACTTTCGGCGCAGGCCGGTCCCGACGGTGCCAAAGCCGCCACCTCCGCCAACGCATACGGCGTCTCCGTGTTCGATCCGGCCGCCGGAAATTGGACCCGAATCGAACCGATCGGAAAGTACCCGAACGGGCTGCCGCTGTTCTGCCGGATAATCAGCTCGAAGGGGAAATTGGTGGTGATCGGCGGGTGGGACCCGGCGACATACCGGCCGGTGGAGGACGTCTTCGTGTACGAGTTCGCGACGGAGAGGTGGAGGCAGGGGAAGGCGATGCCTGAGAGGAGGTCGTTCTTCGGCGCCGCCGCGCACGGCGGCGAAATCTTCGTGGCCGGTGGCCACGACGAGGGGAAGAACGCGGCGGCGACGGCGTGGGCGTACGACGTCAGACAGGACGAGTGGAGGGAGCTGCCGGCGATGGGGGCGGCGCGGGACGAGTGCGAGGCGGTGGCGATCGGATCGGAGATATGGGTGGTTAGCGGTTACGTGACGGAAAATCAAGGGGCGTTCGTCGATAGCGCTGAGGTTTTCGACACAAAGACGGGGACGTGGCGGCGCGTGGACGGCGCGTGGAAGGGGGAGAGGGGTCCGAGGGGGGTGGTGGGAGTGAGGGGAGGCGGAGGAATTTATAATTGGGCGGAGGCGGCGGCGGCGACGGCGAAGGAGGGGATGGTCGGAGTAGAATTGGGGGAAAGGGCGATGGTATTTGGGTGGGAGTATTTGGGAGGGAAGTATGGAATTTTTATGAGGGAAGGGCAAAATGGTAAATTCGAGAGGATGGAAGTTCCTTCGGAATTCTCGGGGTTTGTCCAGTCAGCTTGTTACACGGAGATT

Protein sequence

EKFSATKTMTMAELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQSLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKTGTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAAATAKEGMVGVELGERAMVFGWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI

Homology

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BLAST of MS022954.1 vs. NCBI nr
Match: XP_038904459.1 (F-box/kelch-repeat protein At2g44130-like [Benincasa hispida])

HSP 1 Score: 504.2 bits (1297), Expect = 8.9e-139
Identity = 254/341 (74.49%), Postives = 278/341 (81.52%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIALECLTRSH+TTHRVAARV RRWR LFL  DFY LR  SGRTHKAV  +Q
Sbjct: 4   ELIPGLPEEIALECLTRSHFTTHRVAARVSRRWRHLFLSRDFYNLRKLSGRTHKAVFAVQ 63

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL A    DGAK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCR+I   GKLVV
Sbjct: 64  SLPAPVS-DGAKSAVPV-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRLIGVDGKLVV 123

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDPA++RPVEDVFVYEFA E+WRQGK MPE+RSFFGAA +GGEIFVAGGHDEGKNAA
Sbjct: 124 IGGWDPASFRPVEDVFVYEFAAEKWRQGKGMPEKRSFFGAAEYGGEIFVAGGHDEGKNAA 183

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           ATAWAY+VR DEWRELPAM   RDECEAVAIGSEIWVVSGY TE QG F  +AEVFDTKT
Sbjct: 184 ATAWAYNVRNDEWRELPAMSGGRDECEAVAIGSEIWVVSGYGTEIQGNFETTAEVFDTKT 243

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAAAT----AKEGMVGVELGERAMVFG 312
           G WRRVD AW GER PRGVVGV   G +++WAE  AAT      EG+VGV + ++AMVF 
Sbjct: 244 GEWRRVDSAWCGERSPRGVVGVGREGELFSWAEVMAATTTAVTAEGIVGVNMMDKAMVF- 303

Query: 313 WEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
                G+YGIF  EGQNGKFE++EVP EFSGFVQSACYTEI
Sbjct: 304 ----VGRYGIFTAEGQNGKFEKIEVPEEFSGFVQSACYTEI 337

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BLAST of MS022954.1 vs. NCBI nr
Match: XP_004136552.3 (F-box/kelch-repeat protein At2g44130 [Cucumis sativus])

HSP 1 Score: 474.2 bits (1219), Expect = 9.9e-130
Identity = 240/342 (70.18%), Postives = 268/342 (78.36%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIALECLTRSH+TTHRVAARV RRW +LFL   FY LR  SGRTHKAV  +Q
Sbjct: 6   ELIPGLPEEIALECLTRSHFTTHRVAARVSRRWHRLFLSRHFYNLRKLSGRTHKAVFAVQ 65

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL  Q   D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   GKL V
Sbjct: 66  SL-LQPVSDEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVDGKLAV 125

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDP +YRPVEDVFVYEFA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHDEGKNAA
Sbjct: 126 IGGWDPVSYRPVEDVFVYEFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDEGKNAA 185

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           A+AW Y++R DEWRELPAM   RDECEAVAIGSEIWVVSGY TENQG F  +AEV++TKT
Sbjct: 186 ASAWVYNIRNDEWRELPAMSRGRDECEAVAIGSEIWVVSGYETENQGNFERTAEVYETKT 245

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAA-----ATAKEGMVGVELGERAMVF 312
           G WRRV+ AW  ER PR VVGV   G ++NWA AAA     A   EG+VGV + E+A+VF
Sbjct: 246 GKWRRVESAWCEERSPRNVVGVGREGELFNWATAAAAAKTTAVTGEGIVGVNMEEKAIVF 305

Query: 313 GWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
                 G+ G+FM E QNGK ER+E+P EFSGFVQSACYT I
Sbjct: 306 -----MGRNGVFMGECQNGKLERIELPEEFSGFVQSACYTHI 340

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BLAST of MS022954.1 vs. NCBI nr
Match: KGN59267.1 (hypothetical protein Csa_002357 [Cucumis sativus])

HSP 1 Score: 474.2 bits (1219), Expect = 9.9e-130
Identity = 240/342 (70.18%), Postives = 268/342 (78.36%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIALECLTRSH+TTHRVAARV RRW +LFL   FY LR  SGRTHKAV  +Q
Sbjct: 14  ELIPGLPEEIALECLTRSHFTTHRVAARVSRRWHRLFLSRHFYNLRKLSGRTHKAVFAVQ 73

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL  Q   D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   GKL V
Sbjct: 74  SL-LQPVSDEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVDGKLAV 133

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDP +YRPVEDVFVYEFA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHDEGKNAA
Sbjct: 134 IGGWDPVSYRPVEDVFVYEFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDEGKNAA 193

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           A+AW Y++R DEWRELPAM   RDECEAVAIGSEIWVVSGY TENQG F  +AEV++TKT
Sbjct: 194 ASAWVYNIRNDEWRELPAMSRGRDECEAVAIGSEIWVVSGYETENQGNFERTAEVYETKT 253

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAA-----ATAKEGMVGVELGERAMVF 312
           G WRRV+ AW  ER PR VVGV   G ++NWA AAA     A   EG+VGV + E+A+VF
Sbjct: 254 GKWRRVESAWCEERSPRNVVGVGREGELFNWATAAAAAKTTAVTGEGIVGVNMEEKAIVF 313

Query: 313 GWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
                 G+ G+FM E QNGK ER+E+P EFSGFVQSACYT I
Sbjct: 314 -----MGRNGVFMGECQNGKLERIELPEEFSGFVQSACYTHI 348

[Close]

BLAST of MS022954.1 vs. NCBI nr
Match: XP_008443007.1 (PREDICTED: F-box/kelch-repeat protein At2g44130-like [Cucumis melo] >KAA0043760.1 F-box/kelch-repeat protein [Cucumis melo var. makuwa] >TYK25373.1 F-box/kelch-repeat protein [Cucumis melo var. makuwa])

HSP 1 Score: 471.9 bits (1213), Expect = 4.9e-129
Identity = 243/346 (70.23%), Postives = 271/346 (78.32%), Query Frame = 0


Query: 9   MTMA-ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKA 68
           M MA ELIPG+PEEIALECLTRSH+TTHR+AARV RRWR+LFL   FY LR  SGRTHKA
Sbjct: 2   MDMADELIPGLPEEIALECLTRSHFTTHRLAARVSRRWRRLFLSRHFYNLRKLSGRTHKA 61

Query: 69  VSLIQSLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSK 128
           V  +QSL      D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   
Sbjct: 62  VFAVQSLPLPVS-DEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVD 121

Query: 129 GKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDE 188
           GKL VIGGWDP +YRPVEDVFVY+FA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHDE
Sbjct: 122 GKLAVIGGWDPVSYRPVEDVFVYDFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDE 181

Query: 189 GKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEV 248
           GKNAAATAW Y++R DEWRELPAMGA RDECEAVAIGSEIWVVSGY TENQG F  +AEV
Sbjct: 182 GKNAAATAWVYNIRNDEWRELPAMGAGRDECEAVAIGSEIWVVSGYETENQGDFERTAEV 241

Query: 249 FDTKTGTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAAATAK---EGMVGVELGER- 308
           ++TKTG WRRV+ AW  ER PR VVGV   G ++NWA  AA T     EG+VGV + E+ 
Sbjct: 242 YETKTGKWRRVENAWCEERSPRNVVGVGREGELFNWATVAAKTTAVEGEGIVGVNMEEKG 301

Query: 309 AMVFGWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
           AMVF      G+ G+FM EGQ GK ER+E+P EFSGFVQSA YT I
Sbjct: 302 AMVF-----MGRNGVFMGEGQYGKLERIELPEEFSGFVQSASYTHI 340

[Close]

BLAST of MS022954.1 vs. NCBI nr
Match: XP_023528326.1 (F-box/kelch-repeat protein At2g44130-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 470.3 bits (1209), Expect = 1.4e-128
Identity = 235/340 (69.12%), Postives = 267/340 (78.53%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIAL+CL RSH+TTHR+AARVCRRWR LFL  DFY LR  S RTHKAV  +Q
Sbjct: 4   ELIPGLPEEIALDCLIRSHFTTHRIAARVCRRWRGLFLSRDFYNLRRISDRTHKAVFAVQ 63

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL   AG DG K +T+  A+G+S FDPA+G WT I+PIGKYPNGLPLFCR+I   GKLVV
Sbjct: 64  SLLLPAG-DGDK-STAPPAFGLSAFDPASGKWTWIKPIGKYPNGLPLFCRLIGVDGKLVV 123

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDPA+YRPVEDVFVY+ A E+WRQGK MP  RSFF A   GGEIFVAGGHDE KNAA
Sbjct: 124 IGGWDPASYRPVEDVFVYDIAAEKWRQGKEMPATRSFFSAVECGGEIFVAGGHDEWKNAA 183

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           +TAWAY+++ DEWRELPAM   RDECEAVAIGS+IWVVSGY TENQG F  SAE+F+T+T
Sbjct: 184 STAWAYNIKNDEWRELPAMSCGRDECEAVAIGSDIWVVSGYRTENQGNFEASAEIFNTET 243

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWA---EAAAATAKEGMVGVELGERAMVFGW 312
             WRRVD AW+ ER PRGVVGV   G +++WA   E   A   EG+VGV +  +AMVF  
Sbjct: 244 EEWRRVDSAWREERSPRGVVGVSREGELFSWAAAVETTTAVTAEGVVGVNMEGKAMVF-- 303

Query: 313 EYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
               G++GIF REGQN KFERMEVP EF GFVQSACYTEI
Sbjct: 304 ---VGRHGIFTREGQNSKFERMEVPEEFRGFVQSACYTEI 336

[Close]

BLAST of MS022954.1 vs. ExPASy Swiss-Prot
Match: O80582 (F-box/kelch-repeat protein At2g44130 OS=Arabidopsis thaliana OX=3702 GN=At2g44130 PE=2 SV=2)

HSP 1 Score: 192.2 bits (487), Expect = 9.9e-48
Identity = 108/283 (38.16%), Postives = 154/283 (54.42%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+P E+ALECL R  +        VCR WR L     F + R + G+T   + L+Q
Sbjct: 18  ELIPGLPSELALECLVRVPFQFQSAMRSVCRSWRSLLSDSSFIQERRRCGKTELLLCLVQ 77

Query: 73  SLSAQAGP----------DGAKAATSAN-------AYGVSVFDPAAGNWTRIEPIGKYP- 132
            L+               D  K+   ++        +G+SV++ A   W R+     +P 
Sbjct: 78  PLTPPIPASKSVDETLMVDEKKSEDESHPRVFCTPRFGLSVYNAAMSTWHRV----AFPE 137

Query: 133 -NGLPLFCR--IISSKGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFG 192
              +PLFC   ++   GK+++IGGWDP T +P  DV+V EFA  +WR+G  M E RSFF 
Sbjct: 138 EEQIPLFCECVVLQDAGKILLIGGWDPETLQPTRDVYVLEFAGRKWRRGAPMKESRSFFA 197

Query: 193 -AAAHGGEIFVAGGHDEGKNAAATAWAYDVRQDEWRELPAMGAARDECE--AVAIGSEIW 252
            A+    +++VAGGHD+ KNA  +A  YDV +DEW  +  M   RDEC+  AV +G    
Sbjct: 198 CASVSPTKVYVAGGHDDQKNALRSAEVYDVEKDEWSSVTPMTEGRDECQGFAVGMGLRFC 257

Query: 253 VVSGYVTENQGAFVDSAEVFDTKTGTWRRVDGAWK-GERGPRG 271
           V+SGY TE+QG F    E++D  T +W R+D  W+  +  PRG
Sbjct: 258 VLSGYGTESQGRFRSDGEIYDPATDSWSRIDNVWRFPDTSPRG 296

[Close]

BLAST of MS022954.1 vs. ExPASy Swiss-Prot
Match: Q9LMR5 (F-box/kelch-repeat protein At1g15670 OS=Arabidopsis thaliana OX=3702 GN=At1g15670 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.3e-47
Identity = 108/251 (43.03%), Postives = 140/251 (55.78%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIP +PE +A ECL RS Y    + A VC+ W++     DF++ R  SG + + V L  
Sbjct: 2   ELIPDLPETVAYECLLRSSYKQFPLMASVCKLWQREISLSDFFRHRKASGHSQELVVL-- 61

Query: 73  SLSAQAGPDGAKAATSAN------AYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISS 132
              +QA  D  K   S N       Y +SV +   G  + + P+  + NGLPLFCR++S 
Sbjct: 62  ---SQARVDPVKELVSGNKTIPTPVYRISVLELGTGLRSELPPVPGHSNGLPLFCRLVSV 121

Query: 133 KGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMP-ERRSFFGAAAHG-GEIFVAGG 192
              LVV+ G DP T+R  + VFV+ F T  WR GK+MP   RSFF  A+     +FVAGG
Sbjct: 122 GSDLVVLCGLDPVTWRTSDSVFVFSFLTSTWRVGKSMPGGPRSFFACASDSQRNVFVAGG 181

Query: 193 HDEGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDS 252
           HDE KNA  +A  YDV +D W  LP MG  RDEC A+    +  V+ GY TE QG F  +
Sbjct: 182 HDEDKNAMMSALVYDVAEDRWAFLPDMGRERDECTAIFHAGKFHVIGGYSTEEQGQFSKT 241

Query: 253 AEVFDTKTGTW 256
           AE FD  T  W
Sbjct: 242 AESFDVTTWRW 247

[Close]

BLAST of MS022954.1 vs. ExPASy Swiss-Prot
Match: Q9M8L2 (F-box/kelch-repeat protein At1g80440 OS=Arabidopsis thaliana OX=3702 GN=At1g80440 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.9e-47
Identity = 103/245 (42.04%), Postives = 136/245 (55.51%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIP +P+++A ECL RS Y    V A VCR W +      F   R  S  + + + L Q
Sbjct: 2   ELIPNLPDDVARECLLRSSYQQFPVIASVCRAWNREVSLSQFLHQRKASRHSQELLILSQ 61

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
              A+  P G+    +   Y +SV +  +G WT + PI     GLPLFCR++S    L+V
Sbjct: 62  ---ARVDPAGSGKIIATPEYRISVLESGSGLWTELPPIPGQTKGLPLFCRLVSVGSDLIV 121

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMP-ERRSFFGAAAHGGE-IFVAGGHDEGKN 192
           +GG DP T++  + VFV+ F T +WR G  MP  RRSFFG A+     + VAGGH+E K 
Sbjct: 122 LGGLDPITWQAHDSVFVFSFLTSKWRVGATMPGVRRSFFGCASDSDRTVLVAGGHNEEKC 181

Query: 193 AAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDT 252
           A  +A  YDV +D+W  LP M   RDEC+AV       V+ GY TE QG F  +AE FD 
Sbjct: 182 ALTSAMVYDVSEDKWTFLPDMARERDECKAVFHAGRFHVIGGYATEEQGQFSKTAESFDV 241

Query: 253 KTGTW 256
            T  W
Sbjct: 242 STWEW 243

[Close]

BLAST of MS022954.1 vs. ExPASy Swiss-Prot
Match: Q9M1Y1 (F-box/kelch-repeat protein SKIP20 OS=Arabidopsis thaliana OX=3702 GN=SKIP20 PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 6.6e-44
Identity = 105/294 (35.71%), Postives = 152/294 (51.70%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           +LIPG+PEE+A+ECL R  +  H     VCR W+ +     F K R   G+    + L+Q
Sbjct: 14  DLIPGLPEELAIECLVRVPFQFHSSIKSVCRSWKCVISSRSFIKERIGFGKAESLLCLVQ 73

Query: 73  SLSAQAGP---DGAKAA----------------------TSANAYGVSVFDPAAGNWTRI 132
            L++   P   +G + +                      T    YG+SV++     W R+
Sbjct: 74  PLTSPPSPAMMEGGEMSQKKKEEEEGESQMTQQLLQPRITGTPLYGLSVYNATLDTWHRV 133

Query: 133 EPIGKYPNGLPLFCR--IISSKGKLVVIGGWDPATYRPVEDVFVYEF-----ATERWRQG 192
                 P  +PLFC    I   GK+++IGGWDP T +PV DVFV +F     +  R+R+G
Sbjct: 134 ----AIPERIPLFCECVAIQDAGKVLLIGGWDPETLQPVRDVFVLDFFAGEGSGRRFRRG 193

Query: 193 KAMPERRSFFGAAAHGG-EIFVAGGHDEGKNAAATAWAYDVRQDEWRELPAMGAARDECE 252
           + M   RSFF  A+ G  +++VAGGHD+ KNA  +A  YDV +DEW  LP M   RDEC 
Sbjct: 194 RPMSAARSFFACASVGSTKVYVAGGHDDQKNALRSAEVYDVEKDEWSMLPPMTEGRDECH 253

Query: 253 AVAIGSE--IWVVSGYVTENQGAFVDSAEVFDTKTGTWRRVDGAWK-GERGPRG 271
             ++ ++    V+SGY TE QG F    E++D  T +W  ++  W   +  PRG
Sbjct: 254 GFSMATDPGFCVLSGYGTETQGQFRSDGEIYDPITNSWSTIENVWPFPDLSPRG 303

[Close]

BLAST of MS022954.1 vs. ExPASy Swiss-Prot
Match: Q93W93 (F-box/kelch-repeat protein At1g55270 OS=Arabidopsis thaliana OX=3702 GN=At1g55270 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 5.8e-16
Identity = 70/265 (26.42%), Postives = 111/265 (41.89%), Query Frame = 0


Query: 7   KTMTMAELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHK 66
           +T     L+PG+P+++A+ CL R     HR    VC+RW +L  G  FY  R   G + +
Sbjct: 71  RTRIQPPLLPGLPDDLAVACLIRVPRAEHRKLRLVCKRWYRLASGNFFYSQRKLLGMSEE 130

Query: 67  AVSLIQSLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGK-YPNGLPLFCRIIS 126
            V +      +   DG            + FDP +  W  + P+ + Y   +   C ++S
Sbjct: 131 WVYVF-----KRDRDG--------KISWNTFDPISQLWQPLPPVPREYSEAVGFGCAVLS 190

Query: 127 SKGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGH 186
               L + GG DP     +  V  Y   T +W +   M  +R FFG       ++VAGG 
Sbjct: 191 G-CHLYLFGGKDPLR-GSMRRVIFYNARTNKWHRAPDMLRKRHFFGCCVINNCLYVAGGE 250

Query: 187 DEG-KNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDS 246
            EG +    +A  YD  ++ W  +  M  A      V    + W + G  +      +  
Sbjct: 251 CEGIQRTLRSAEVYDPNKNRWSFIADMSTAMVPLIGVVYDKK-WFLKGLGSHQ----LVM 310

Query: 247 AEVFDTKTGTWRRV-DGAWKGERGP 269
           +E +D +  +W  V DG   G R P
Sbjct: 311 SEAYDPEVNSWSPVSDGMVAGWRNP 315

[Close]

BLAST of MS022954.1 vs. ExPASy TrEMBL
Match: A0A0A0LEY5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G792070 PE=4 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 4.8e-130
Identity = 240/342 (70.18%), Postives = 268/342 (78.36%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIALECLTRSH+TTHRVAARV RRW +LFL   FY LR  SGRTHKAV  +Q
Sbjct: 14  ELIPGLPEEIALECLTRSHFTTHRVAARVSRRWHRLFLSRHFYNLRKLSGRTHKAVFAVQ 73

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL  Q   D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   GKL V
Sbjct: 74  SL-LQPVSDEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVDGKLAV 133

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDP +YRPVEDVFVYEFA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHDEGKNAA
Sbjct: 134 IGGWDPVSYRPVEDVFVYEFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDEGKNAA 193

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           A+AW Y++R DEWRELPAM   RDECEAVAIGSEIWVVSGY TENQG F  +AEV++TKT
Sbjct: 194 ASAWVYNIRNDEWRELPAMSRGRDECEAVAIGSEIWVVSGYETENQGNFERTAEVYETKT 253

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAA-----ATAKEGMVGVELGERAMVF 312
           G WRRV+ AW  ER PR VVGV   G ++NWA AAA     A   EG+VGV + E+A+VF
Sbjct: 254 GKWRRVESAWCEERSPRNVVGVGREGELFNWATAAAAAKTTAVTGEGIVGVNMEEKAIVF 313

Query: 313 GWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
                 G+ G+FM E QNGK ER+E+P EFSGFVQSACYT I
Sbjct: 314 -----MGRNGVFMGECQNGKLERIELPEEFSGFVQSACYTHI 348

[Close]

BLAST of MS022954.1 vs. ExPASy TrEMBL
Match: A0A5D3DP02 (F-box/kelch-repeat protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005130 PE=4 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.4e-129
Identity = 243/346 (70.23%), Postives = 271/346 (78.32%), Query Frame = 0


Query: 9   MTMA-ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKA 68
           M MA ELIPG+PEEIALECLTRSH+TTHR+AARV RRWR+LFL   FY LR  SGRTHKA
Sbjct: 2   MDMADELIPGLPEEIALECLTRSHFTTHRLAARVSRRWRRLFLSRHFYNLRKLSGRTHKA 61

Query: 69  VSLIQSLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSK 128
           V  +QSL      D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   
Sbjct: 62  VFAVQSLPLPVS-DEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVD 121

Query: 129 GKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDE 188
           GKL VIGGWDP +YRPVEDVFVY+FA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHDE
Sbjct: 122 GKLAVIGGWDPVSYRPVEDVFVYDFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDE 181

Query: 189 GKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEV 248
           GKNAAATAW Y++R DEWRELPAMGA RDECEAVAIGSEIWVVSGY TENQG F  +AEV
Sbjct: 182 GKNAAATAWVYNIRNDEWRELPAMGAGRDECEAVAIGSEIWVVSGYETENQGDFERTAEV 241

Query: 249 FDTKTGTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAAATAK---EGMVGVELGER- 308
           ++TKTG WRRV+ AW  ER PR VVGV   G ++NWA  AA T     EG+VGV + E+ 
Sbjct: 242 YETKTGKWRRVENAWCEERSPRNVVGVGREGELFNWATVAAKTTAVEGEGIVGVNMEEKG 301

Query: 309 AMVFGWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
           AMVF      G+ G+FM EGQ GK ER+E+P EFSGFVQSA YT I
Sbjct: 302 AMVF-----MGRNGVFMGEGQYGKLERIELPEEFSGFVQSASYTHI 340

[Close]

BLAST of MS022954.1 vs. ExPASy TrEMBL
Match: A0A1S3B7S9 (F-box/kelch-repeat protein At2g44130-like OS=Cucumis melo OX=3656 GN=LOC103486736 PE=4 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.4e-129
Identity = 243/346 (70.23%), Postives = 271/346 (78.32%), Query Frame = 0


Query: 9   MTMA-ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKA 68
           M MA ELIPG+PEEIALECLTRSH+TTHR+AARV RRWR+LFL   FY LR  SGRTHKA
Sbjct: 2   MDMADELIPGLPEEIALECLTRSHFTTHRLAARVSRRWRRLFLSRHFYNLRKLSGRTHKA 61

Query: 69  VSLIQSLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSK 128
           V  +QSL      D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   
Sbjct: 62  VFAVQSLPLPVS-DEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVD 121

Query: 129 GKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDE 188
           GKL VIGGWDP +YRPVEDVFVY+FA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHDE
Sbjct: 122 GKLAVIGGWDPVSYRPVEDVFVYDFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDE 181

Query: 189 GKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEV 248
           GKNAAATAW Y++R DEWRELPAMGA RDECEAVAIGSEIWVVSGY TENQG F  +AEV
Sbjct: 182 GKNAAATAWVYNIRNDEWRELPAMGAGRDECEAVAIGSEIWVVSGYETENQGDFERTAEV 241

Query: 249 FDTKTGTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAAATAK---EGMVGVELGER- 308
           ++TKTG WRRV+ AW  ER PR VVGV   G ++NWA  AA T     EG+VGV + E+ 
Sbjct: 242 YETKTGKWRRVENAWCEERSPRNVVGVGREGELFNWATVAAKTTAVEGEGIVGVNMEEKG 301

Query: 309 AMVFGWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
           AMVF      G+ G+FM EGQ GK ER+E+P EFSGFVQSA YT I
Sbjct: 302 AMVF-----MGRNGVFMGEGQYGKLERIELPEEFSGFVQSASYTHI 340

[Close]

BLAST of MS022954.1 vs. ExPASy TrEMBL
Match: A0A6J1J0Y8 (F-box/kelch-repeat protein At2g44130-like OS=Cucurbita maxima OX=3661 GN=LOC111481691 PE=4 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 2.2e-127
Identity = 233/340 (68.53%), Postives = 267/340 (78.53%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIAL+CL RSH+TTHR+AARVCRRWR LFL  DF+ LR  S RTHKAV  +Q
Sbjct: 4   ELIPGLPEEIALDCLIRSHFTTHRIAARVCRRWRGLFLSRDFHNLRRISDRTHKAVFAVQ 63

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL   AG DG K +T+  A+G+S FDPA G WT I+PI KY NGLPLFCR+I   GKLVV
Sbjct: 64  SLLVPAG-DGDK-STAPLAFGLSAFDPATGKWTWIKPIEKYSNGLPLFCRLIGVDGKLVV 123

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDPA+YRPVEDVFVY+ A E+WRQGK MP  RSFF A   GGEIFVAGGHDE KNAA
Sbjct: 124 IGGWDPASYRPVEDVFVYDMAAEKWRQGKGMPATRSFFSAVECGGEIFVAGGHDEWKNAA 183

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           +TAWAY+++ DEWRELPAM   RDECEAVAIGS+IWVVSGY TENQG F  +AE+FDT+T
Sbjct: 184 STAWAYNIKNDEWRELPAMSCGRDECEAVAIGSDIWVVSGYRTENQGNFEATAEIFDTET 243

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAAAT---AKEGMVGVELGERAMVFGW 312
             WRRVD AW+ ER PRGVVGV   GG+++WA A AAT     E +VGV +  +AMVF  
Sbjct: 244 EKWRRVDSAWREERSPRGVVGVSREGGLFSWAAAVAATTAVTAEEVVGVNMEGKAMVF-- 303

Query: 313 EYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
               G++GIF REGQN KFERMEVP +F+GFVQSACYTEI
Sbjct: 304 ---VGRHGIFTREGQNSKFERMEVPEDFTGFVQSACYTEI 336

[Close]

BLAST of MS022954.1 vs. ExPASy TrEMBL
Match: A0A6J1F4W9 (F-box/kelch-repeat protein At2g44130-like OS=Cucurbita moschata OX=3662 GN=LOC111442196 PE=4 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 6.1e-125
Identity = 228/340 (67.06%), Postives = 262/340 (77.06%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIAL+CL RSH+TTHR+AARVCRRWR LFL  DFY LR  S RTHK V  +Q
Sbjct: 4   ELIPGLPEEIALDCLIRSHFTTHRIAARVCRRWRGLFLSRDFYNLRRISDRTHKVVFAVQ 63

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL   AG DG K +T+  A+G+S FDPA G WT I+PI KYPN LPLFCR+I   GKLVV
Sbjct: 64  SLLVPAG-DGDK-STAPPAFGLSAFDPATGKWTWIKPIEKYPNRLPLFCRLIGVDGKLVV 123

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDPA+YRPVE+VFVY+ A E+WRQGK MP  RSFF A   GGEIFVAGGHDE KNAA
Sbjct: 124 IGGWDPASYRPVEEVFVYDIAAEKWRQGKGMPATRSFFSAVEFGGEIFVAGGHDEWKNAA 183

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           +TAW Y+++ DEWRELPAM   RDECEAVAIGS+IWVVSGY TENQG F  +AE+F+T+T
Sbjct: 184 STAWVYNIKNDEWRELPAMSYGRDECEAVAIGSDIWVVSGYRTENQGNFEATAEIFNTET 243

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWA---EAAAATAKEGMVGVELGERAMVFGW 312
             WRRVD AW+ ER PRGVVGV   G +++WA   E   A   EG+VGV +  +AMVF  
Sbjct: 244 EEWRRVDSAWREERSPRGVVGVSREGELFSWAAAVETTTAVTAEGVVGVTMEGKAMVF-- 303

Query: 313 EYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
               G++GIF REG N KFERMEVP EF+GFVQSACYTEI
Sbjct: 304 ---VGRHGIFTREGHNSKFERMEVPEEFTGFVQSACYTEI 336

[Close]

BLAST of MS022954.1 vs. TAIR 10
Match: AT2G44130.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 192.2 bits (487), Expect = 7.0e-49
Identity = 108/283 (38.16%), Postives = 154/283 (54.42%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+P E+ALECL R  +        VCR WR L     F + R + G+T   + L+Q
Sbjct: 18  ELIPGLPSELALECLVRVPFQFQSAMRSVCRSWRSLLSDSSFIQERRRCGKTELLLCLVQ 77

Query: 73  SLSAQAGP----------DGAKAATSAN-------AYGVSVFDPAAGNWTRIEPIGKYP- 132
            L+               D  K+   ++        +G+SV++ A   W R+     +P 
Sbjct: 78  PLTPPIPASKSVDETLMVDEKKSEDESHPRVFCTPRFGLSVYNAAMSTWHRV----AFPE 137

Query: 133 -NGLPLFCR--IISSKGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFG 192
              +PLFC   ++   GK+++IGGWDP T +P  DV+V EFA  +WR+G  M E RSFF 
Sbjct: 138 EEQIPLFCECVVLQDAGKILLIGGWDPETLQPTRDVYVLEFAGRKWRRGAPMKESRSFFA 197

Query: 193 -AAAHGGEIFVAGGHDEGKNAAATAWAYDVRQDEWRELPAMGAARDECE--AVAIGSEIW 252
            A+    +++VAGGHD+ KNA  +A  YDV +DEW  +  M   RDEC+  AV +G    
Sbjct: 198 CASVSPTKVYVAGGHDDQKNALRSAEVYDVEKDEWSSVTPMTEGRDECQGFAVGMGLRFC 257

Query: 253 VVSGYVTENQGAFVDSAEVFDTKTGTWRRVDGAWK-GERGPRG 271
           V+SGY TE+QG F    E++D  T +W R+D  W+  +  PRG
Sbjct: 258 VLSGYGTESQGRFRSDGEIYDPATDSWSRIDNVWRFPDTSPRG 296

[Close]

BLAST of MS022954.1 vs. TAIR 10
Match: AT1G15670.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 191.8 bits (486), Expect = 9.2e-49
Identity = 108/251 (43.03%), Postives = 140/251 (55.78%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIP +PE +A ECL RS Y    + A VC+ W++     DF++ R  SG + + V L  
Sbjct: 2   ELIPDLPETVAYECLLRSSYKQFPLMASVCKLWQREISLSDFFRHRKASGHSQELVVL-- 61

Query: 73  SLSAQAGPDGAKAATSAN------AYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISS 132
              +QA  D  K   S N       Y +SV +   G  + + P+  + NGLPLFCR++S 
Sbjct: 62  ---SQARVDPVKELVSGNKTIPTPVYRISVLELGTGLRSELPPVPGHSNGLPLFCRLVSV 121

Query: 133 KGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMP-ERRSFFGAAAHG-GEIFVAGG 192
              LVV+ G DP T+R  + VFV+ F T  WR GK+MP   RSFF  A+     +FVAGG
Sbjct: 122 GSDLVVLCGLDPVTWRTSDSVFVFSFLTSTWRVGKSMPGGPRSFFACASDSQRNVFVAGG 181

Query: 193 HDEGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDS 252
           HDE KNA  +A  YDV +D W  LP MG  RDEC A+    +  V+ GY TE QG F  +
Sbjct: 182 HDEDKNAMMSALVYDVAEDRWAFLPDMGRERDECTAIFHAGKFHVIGGYSTEEQGQFSKT 241

Query: 253 AEVFDTKTGTW 256
           AE FD  T  W
Sbjct: 242 AESFDVTTWRW 247

[Close]

BLAST of MS022954.1 vs. TAIR 10
Match: AT1G80440.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 190.7 bits (483), Expect = 2.0e-48
Identity = 103/245 (42.04%), Postives = 136/245 (55.51%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIP +P+++A ECL RS Y    V A VCR W +      F   R  S  + + + L Q
Sbjct: 2   ELIPNLPDDVARECLLRSSYQQFPVIASVCRAWNREVSLSQFLHQRKASRHSQELLILSQ 61

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
              A+  P G+    +   Y +SV +  +G WT + PI     GLPLFCR++S    L+V
Sbjct: 62  ---ARVDPAGSGKIIATPEYRISVLESGSGLWTELPPIPGQTKGLPLFCRLVSVGSDLIV 121

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMP-ERRSFFGAAAHGGE-IFVAGGHDEGKN 192
           +GG DP T++  + VFV+ F T +WR G  MP  RRSFFG A+     + VAGGH+E K 
Sbjct: 122 LGGLDPITWQAHDSVFVFSFLTSKWRVGATMPGVRRSFFGCASDSDRTVLVAGGHNEEKC 181

Query: 193 AAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDT 252
           A  +A  YDV +D+W  LP M   RDEC+AV       V+ GY TE QG F  +AE FD 
Sbjct: 182 ALTSAMVYDVSEDKWTFLPDMARERDECKAVFHAGRFHVIGGYATEEQGQFSKTAESFDV 241

Query: 253 KTGTW 256
            T  W
Sbjct: 242 STWEW 243

[Close]

BLAST of MS022954.1 vs. TAIR 10
Match: AT3G59940.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 4.7e-45
Identity = 105/294 (35.71%), Postives = 152/294 (51.70%), Query Frame = 0


Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           +LIPG+PEE+A+ECL R  +  H     VCR W+ +     F K R   G+    + L+Q
Sbjct: 14  DLIPGLPEELAIECLVRVPFQFHSSIKSVCRSWKCVISSRSFIKERIGFGKAESLLCLVQ 73

Query: 73  SLSAQAGP---DGAKAA----------------------TSANAYGVSVFDPAAGNWTRI 132
            L++   P   +G + +                      T    YG+SV++     W R+
Sbjct: 74  PLTSPPSPAMMEGGEMSQKKKEEEEGESQMTQQLLQPRITGTPLYGLSVYNATLDTWHRV 133

Query: 133 EPIGKYPNGLPLFCR--IISSKGKLVVIGGWDPATYRPVEDVFVYEF-----ATERWRQG 192
                 P  +PLFC    I   GK+++IGGWDP T +PV DVFV +F     +  R+R+G
Sbjct: 134 ----AIPERIPLFCECVAIQDAGKVLLIGGWDPETLQPVRDVFVLDFFAGEGSGRRFRRG 193

Query: 193 KAMPERRSFFGAAAHGG-EIFVAGGHDEGKNAAATAWAYDVRQDEWRELPAMGAARDECE 252
           + M   RSFF  A+ G  +++VAGGHD+ KNA  +A  YDV +DEW  LP M   RDEC 
Sbjct: 194 RPMSAARSFFACASVGSTKVYVAGGHDDQKNALRSAEVYDVEKDEWSMLPPMTEGRDECH 253

Query: 253 AVAIGSE--IWVVSGYVTENQGAFVDSAEVFDTKTGTWRRVDGAWK-GERGPRG 271
             ++ ++    V+SGY TE QG F    E++D  T +W  ++  W   +  PRG
Sbjct: 254 GFSMATDPGFCVLSGYGTETQGQFRSDGEIYDPITNSWSTIENVWPFPDLSPRG 303

[Close]

BLAST of MS022954.1 vs. TAIR 10
Match: AT1G16250.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 4.1e-17
Identity = 75/296 (25.34%), Postives = 128/296 (43.24%), Query Frame = 0


Query: 14  LIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQS 73
           +IPG+P+++AL C+ +  +  H V   V R WR L  G D+   + ++G +   + ++  
Sbjct: 8   IIPGLPDDLALRCIAKLSHGYHGVLECVSRGWRDLVRGADYSCYKARNGWSGSWLFVL-- 67

Query: 74  LSAQAGPDGAKAATSANAYGVSVFDPAAGNW-----TRIEPIGKYPNGLPLFCRIISSKG 133
                        T  +      +DP A  W     TR    G + +G    C    S  
Sbjct: 68  -------------TERSKNQWVAYDPEADRWHPLPRTRAVQDGWHHSGFACVC---VSNC 127

Query: 134 KLVVIGGWDPAT-----YRPV--EDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFV 193
            LV+ G + P+       +PV  +DV  ++   ++W+   +M   R+ F   +  G+++V
Sbjct: 128 LLVIGGCYAPSVSSFPHQKPVVTKDVMRFDPFKKQWKMVASMRTPRTHFACTSVSGKVYV 187

Query: 194 AGGHD-EGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVS---GYVTEN 253
           AGG +        +A  YD   D W ELPAM   + +C  ++      V+S   G+  +N
Sbjct: 188 AGGRNLTHSRGIPSAEVYDPVADRWEELPAMPRPQMDCSGLSYRGCFHVLSDQVGFAEQN 247

Query: 254 QGAFVDSAEVFDTKTGTWRRVDGAWKGERGPRGVVGVRGGGGIY---NWAEAAAAT 291
                 S+EVF+ +  TW  V+  W   R  +  V V     +Y   +W E+   T
Sbjct: 248 ------SSEVFNPRDMTWSTVEDVWPFSRAMQFAVQVMKNDRVYTIVDWGESLIKT 279

The following BLAST results are available for this feature:

BLAST of MS022954.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904459.1	8.9e-139	74.49	F-box/kelch-repeat protein At2g44130-like [Benincasa hispida]	[more]
XP_004136552.3	9.9e-130	70.18	F-box/kelch-repeat protein At2g44130 [Cucumis sativus]	[more]
KGN59267.1	9.9e-130	70.18	hypothetical protein Csa_002357 [Cucumis sativus]	[more]
XP_008443007.1	4.9e-129	70.23	PREDICTED: F-box/kelch-repeat protein At2g44130-like [Cucumis melo] >KAA0043760....	[more]
XP_023528326.1	1.4e-128	69.12	F-box/kelch-repeat protein At2g44130-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS022954.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80582	9.9e-48	38.16	F-box/kelch-repeat protein At2g44130 OS=Arabidopsis thaliana OX=3702 GN=At2g4413...	[more]
Q9LMR5	1.3e-47	43.03	F-box/kelch-repeat protein At1g15670 OS=Arabidopsis thaliana OX=3702 GN=At1g1567...	[more]
Q9M8L2	2.9e-47	42.04	F-box/kelch-repeat protein At1g80440 OS=Arabidopsis thaliana OX=3702 GN=At1g8044...	[more]
Q9M1Y1	6.6e-44	35.71	F-box/kelch-repeat protein SKIP20 OS=Arabidopsis thaliana OX=3702 GN=SKIP20 PE=1...	[more]
Q93W93	5.8e-16	26.42	F-box/kelch-repeat protein At1g55270 OS=Arabidopsis thaliana OX=3702 GN=At1g5527...	[more]

BLAST of MS022954.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LEY5	4.8e-130	70.18	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G792070 PE=4 SV=1	[more]
A0A5D3DP02	2.4e-129	70.23	F-box/kelch-repeat protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3B7S9	2.4e-129	70.23	F-box/kelch-repeat protein At2g44130-like OS=Cucumis melo OX=3656 GN=LOC10348673...	[more]
A0A6J1J0Y8	2.2e-127	68.53	F-box/kelch-repeat protein At2g44130-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F4W9	6.1e-125	67.06	F-box/kelch-repeat protein At2g44130-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of MS022954.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44130.1	7.0e-49	38.16	Galactose oxidase/kelch repeat superfamily protein	[more]
AT1G15670.1	9.2e-49	43.03	Galactose oxidase/kelch repeat superfamily protein	[more]
AT1G80440.1	2.0e-48	42.04	Galactose oxidase/kelch repeat superfamily protein	[more]
AT3G59940.1	4.7e-45	35.71	Galactose oxidase/kelch repeat superfamily protein	[more]
AT1G16250.1	4.1e-17	25.34	Galactose oxidase/kelch repeat superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006652	Kelch repeat type 1	SMART	SM00612	kelc_smart	coord: 226..276 e-value: 18.0 score: 0.6 coord: 178..225 e-value: 2.7E-5 score: 33.6 coord: 129..177 e-value: 8.6E-9 score: 45.2
IPR006652	Kelch repeat type 1	PFAM	PF01344	Kelch_1	coord: 166..211 e-value: 1.1E-12 score: 47.3 coord: 125..164 e-value: 7.6E-7 score: 28.6 coord: 216..258 e-value: 2.0E-5 score: 24.1
IPR015915	Kelch-type beta propeller	GENE3D	2.120.10.80		coord: 63..311 e-value: 9.0E-41 score: 141.7
IPR015915	Kelch-type beta propeller	SUPERFAMILY	117281	Kelch motif	coord: 92..268
IPR044595	F-box/kelch-repeat protein KMD1/2-like	PANTHER	PTHR46407	OS02G0208700 PROTEIN	coord: 10..349
None	No IPR available	PANTHER	PTHR46407:SF6	F-BOX/KELCH-REPEAT PLANT-LIKE PROTEIN	coord: 10..349
IPR036047	F-box-like domain superfamily	SUPERFAMILY	81383	F-box domain	coord: 15..58

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS022954	MS022954	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS022954.1-cds	MS022954.1-cds-scaffold357:76756..77802	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS022954.1	MS022954.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0080037	negative regulation of cytokinin-activated signaling pathway
biological_process	GO:2000762	regulation of phenylpropanoid metabolic process
molecular_function	GO:0005515	protein binding