MS022954 (gene) Bitter gourd (TR) v1

Overview
NameMS022954
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionF-box/kelch-repeat protein
Locationscaffold357: 76756 .. 77802 (-)
RNA-Seq ExpressionMS022954
SyntenyMS022954
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAATTTTCAGCAACAAAAACAATGACTATGGCGGAATTGATTCCTGGTGTACCCGAAGAAATTGCCCTCGAGTGCTTAACTCGGTCCCACTACACGACTCACCGAGTCGCCGCCCGAGTCTGCCGCCGCTGGCGCCAGCTCTTCCTCGGCCCTGATTTCTACAAACTCCGTCACAAATCCGGCCGAACCCACAAGGCGGTCTCCTTAATTCAGTCACTTTCGGCGCAGGCCGGTCCCGACGGTGCCAAAGCCGCCACCTCCGCCAACGCATACGGCGTCTCCGTGTTCGATCCGGCCGCCGGAAATTGGACCCGAATCGAACCGATCGGAAAGTACCCGAACGGGCTGCCGCTGTTCTGCCGGATAATCAGCTCGAAGGGGAAATTGGTGGTGATCGGCGGGTGGGACCCGGCGACATACCGGCCGGTGGAGGACGTCTTCGTGTACGAGTTCGCGACGGAGAGGTGGAGGCAGGGGAAGGCGATGCCTGAGAGGAGGTCGTTCTTCGGCGCCGCCGCGCACGGCGGCGAAATCTTCGTGGCCGGTGGCCACGACGAGGGGAAGAACGCGGCGGCGACGGCGTGGGCGTACGACGTCAGACAGGACGAGTGGAGGGAGCTGCCGGCGATGGGGGCGGCGCGGGACGAGTGCGAGGCGGTGGCGATCGGATCGGAGATATGGGTGGTTAGCGGTTACGTGACGGAAAATCAAGGGGCGTTCGTCGATAGCGCTGAGGTTTTCGACACAAAGACGGGGACGTGGCGGCGCGTGGACGGCGCGTGGAAGGGGGAGAGGGGTCCGAGGGGGGTGGTGGGAGTGAGGGGAGGCGGAGGAATTTATAATTGGGCGGAGGCGGCGGCGGCGACGGCGAAGGAGGGGATGGTCGGAGTAGAATTGGGGGAAAGGGCGATGGTATTTGGGTGGGAGTATTTGGGAGGGAAGTATGGAATTTTTATGAGGGAAGGGCAAAATGGTAAATTCGAGAGGATGGAAGTTCCTTCGGAATTCTCGGGGTTTGTCCAGTCAGCTTGTTACACGGAGATT

mRNA sequence

GAGAAATTTTCAGCAACAAAAACAATGACTATGGCGGAATTGATTCCTGGTGTACCCGAAGAAATTGCCCTCGAGTGCTTAACTCGGTCCCACTACACGACTCACCGAGTCGCCGCCCGAGTCTGCCGCCGCTGGCGCCAGCTCTTCCTCGGCCCTGATTTCTACAAACTCCGTCACAAATCCGGCCGAACCCACAAGGCGGTCTCCTTAATTCAGTCACTTTCGGCGCAGGCCGGTCCCGACGGTGCCAAAGCCGCCACCTCCGCCAACGCATACGGCGTCTCCGTGTTCGATCCGGCCGCCGGAAATTGGACCCGAATCGAACCGATCGGAAAGTACCCGAACGGGCTGCCGCTGTTCTGCCGGATAATCAGCTCGAAGGGGAAATTGGTGGTGATCGGCGGGTGGGACCCGGCGACATACCGGCCGGTGGAGGACGTCTTCGTGTACGAGTTCGCGACGGAGAGGTGGAGGCAGGGGAAGGCGATGCCTGAGAGGAGGTCGTTCTTCGGCGCCGCCGCGCACGGCGGCGAAATCTTCGTGGCCGGTGGCCACGACGAGGGGAAGAACGCGGCGGCGACGGCGTGGGCGTACGACGTCAGACAGGACGAGTGGAGGGAGCTGCCGGCGATGGGGGCGGCGCGGGACGAGTGCGAGGCGGTGGCGATCGGATCGGAGATATGGGTGGTTAGCGGTTACGTGACGGAAAATCAAGGGGCGTTCGTCGATAGCGCTGAGGTTTTCGACACAAAGACGGGGACGTGGCGGCGCGTGGACGGCGCGTGGAAGGGGGAGAGGGGTCCGAGGGGGGTGGTGGGAGTGAGGGGAGGCGGAGGAATTTATAATTGGGCGGAGGCGGCGGCGGCGACGGCGAAGGAGGGGATGGTCGGAGTAGAATTGGGGGAAAGGGCGATGGTATTTGGGTGGGAGTATTTGGGAGGGAAGTATGGAATTTTTATGAGGGAAGGGCAAAATGGTAAATTCGAGAGGATGGAAGTTCCTTCGGAATTCTCGGGGTTTGTCCAGTCAGCTTGTTACACGGAGATT

Coding sequence (CDS)

GAGAAATTTTCAGCAACAAAAACAATGACTATGGCGGAATTGATTCCTGGTGTACCCGAAGAAATTGCCCTCGAGTGCTTAACTCGGTCCCACTACACGACTCACCGAGTCGCCGCCCGAGTCTGCCGCCGCTGGCGCCAGCTCTTCCTCGGCCCTGATTTCTACAAACTCCGTCACAAATCCGGCCGAACCCACAAGGCGGTCTCCTTAATTCAGTCACTTTCGGCGCAGGCCGGTCCCGACGGTGCCAAAGCCGCCACCTCCGCCAACGCATACGGCGTCTCCGTGTTCGATCCGGCCGCCGGAAATTGGACCCGAATCGAACCGATCGGAAAGTACCCGAACGGGCTGCCGCTGTTCTGCCGGATAATCAGCTCGAAGGGGAAATTGGTGGTGATCGGCGGGTGGGACCCGGCGACATACCGGCCGGTGGAGGACGTCTTCGTGTACGAGTTCGCGACGGAGAGGTGGAGGCAGGGGAAGGCGATGCCTGAGAGGAGGTCGTTCTTCGGCGCCGCCGCGCACGGCGGCGAAATCTTCGTGGCCGGTGGCCACGACGAGGGGAAGAACGCGGCGGCGACGGCGTGGGCGTACGACGTCAGACAGGACGAGTGGAGGGAGCTGCCGGCGATGGGGGCGGCGCGGGACGAGTGCGAGGCGGTGGCGATCGGATCGGAGATATGGGTGGTTAGCGGTTACGTGACGGAAAATCAAGGGGCGTTCGTCGATAGCGCTGAGGTTTTCGACACAAAGACGGGGACGTGGCGGCGCGTGGACGGCGCGTGGAAGGGGGAGAGGGGTCCGAGGGGGGTGGTGGGAGTGAGGGGAGGCGGAGGAATTTATAATTGGGCGGAGGCGGCGGCGGCGACGGCGAAGGAGGGGATGGTCGGAGTAGAATTGGGGGAAAGGGCGATGGTATTTGGGTGGGAGTATTTGGGAGGGAAGTATGGAATTTTTATGAGGGAAGGGCAAAATGGTAAATTCGAGAGGATGGAAGTTCCTTCGGAATTCTCGGGGTTTGTCCAGTCAGCTTGTTACACGGAGATT

Protein sequence

EKFSATKTMTMAELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQSLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKTGTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAAATAKEGMVGVELGERAMVFGWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI
Homology
BLAST of MS022954 vs. NCBI nr
Match: XP_038904459.1 (F-box/kelch-repeat protein At2g44130-like [Benincasa hispida])

HSP 1 Score: 504.2 bits (1297), Expect = 8.9e-139
Identity = 254/341 (74.49%), Postives = 278/341 (81.52%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIALECLTRSH+TTHRVAARV RRWR LFL  DFY LR  SGRTHKAV  +Q
Sbjct: 4   ELIPGLPEEIALECLTRSHFTTHRVAARVSRRWRHLFLSRDFYNLRKLSGRTHKAVFAVQ 63

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL A    DGAK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCR+I   GKLVV
Sbjct: 64  SLPAPVS-DGAKSAVPV-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRLIGVDGKLVV 123

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDPA++RPVEDVFVYEFA E+WRQGK MPE+RSFFGAA +GGEIFVAGGHDEGKNAA
Sbjct: 124 IGGWDPASFRPVEDVFVYEFAAEKWRQGKGMPEKRSFFGAAEYGGEIFVAGGHDEGKNAA 183

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           ATAWAY+VR DEWRELPAM   RDECEAVAIGSEIWVVSGY TE QG F  +AEVFDTKT
Sbjct: 184 ATAWAYNVRNDEWRELPAMSGGRDECEAVAIGSEIWVVSGYGTEIQGNFETTAEVFDTKT 243

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAAAT----AKEGMVGVELGERAMVFG 312
           G WRRVD AW GER PRGVVGV   G +++WAE  AAT      EG+VGV + ++AMVF 
Sbjct: 244 GEWRRVDSAWCGERSPRGVVGVGREGELFSWAEVMAATTTAVTAEGIVGVNMMDKAMVF- 303

Query: 313 WEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
                G+YGIF  EGQNGKFE++EVP EFSGFVQSACYTEI
Sbjct: 304 ----VGRYGIFTAEGQNGKFEKIEVPEEFSGFVQSACYTEI 337

BLAST of MS022954 vs. NCBI nr
Match: XP_004136552.3 (F-box/kelch-repeat protein At2g44130 [Cucumis sativus])

HSP 1 Score: 474.2 bits (1219), Expect = 9.9e-130
Identity = 240/342 (70.18%), Postives = 268/342 (78.36%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIALECLTRSH+TTHRVAARV RRW +LFL   FY LR  SGRTHKAV  +Q
Sbjct: 6   ELIPGLPEEIALECLTRSHFTTHRVAARVSRRWHRLFLSRHFYNLRKLSGRTHKAVFAVQ 65

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL  Q   D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   GKL V
Sbjct: 66  SL-LQPVSDEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVDGKLAV 125

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDP +YRPVEDVFVYEFA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHDEGKNAA
Sbjct: 126 IGGWDPVSYRPVEDVFVYEFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDEGKNAA 185

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           A+AW Y++R DEWRELPAM   RDECEAVAIGSEIWVVSGY TENQG F  +AEV++TKT
Sbjct: 186 ASAWVYNIRNDEWRELPAMSRGRDECEAVAIGSEIWVVSGYETENQGNFERTAEVYETKT 245

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAA-----ATAKEGMVGVELGERAMVF 312
           G WRRV+ AW  ER PR VVGV   G ++NWA AAA     A   EG+VGV + E+A+VF
Sbjct: 246 GKWRRVESAWCEERSPRNVVGVGREGELFNWATAAAAAKTTAVTGEGIVGVNMEEKAIVF 305

Query: 313 GWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
                 G+ G+FM E QNGK ER+E+P EFSGFVQSACYT I
Sbjct: 306 -----MGRNGVFMGECQNGKLERIELPEEFSGFVQSACYTHI 340

BLAST of MS022954 vs. NCBI nr
Match: KGN59267.1 (hypothetical protein Csa_002357 [Cucumis sativus])

HSP 1 Score: 474.2 bits (1219), Expect = 9.9e-130
Identity = 240/342 (70.18%), Postives = 268/342 (78.36%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIALECLTRSH+TTHRVAARV RRW +LFL   FY LR  SGRTHKAV  +Q
Sbjct: 14  ELIPGLPEEIALECLTRSHFTTHRVAARVSRRWHRLFLSRHFYNLRKLSGRTHKAVFAVQ 73

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL  Q   D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   GKL V
Sbjct: 74  SL-LQPVSDEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVDGKLAV 133

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDP +YRPVEDVFVYEFA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHDEGKNAA
Sbjct: 134 IGGWDPVSYRPVEDVFVYEFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDEGKNAA 193

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           A+AW Y++R DEWRELPAM   RDECEAVAIGSEIWVVSGY TENQG F  +AEV++TKT
Sbjct: 194 ASAWVYNIRNDEWRELPAMSRGRDECEAVAIGSEIWVVSGYETENQGNFERTAEVYETKT 253

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAA-----ATAKEGMVGVELGERAMVF 312
           G WRRV+ AW  ER PR VVGV   G ++NWA AAA     A   EG+VGV + E+A+VF
Sbjct: 254 GKWRRVESAWCEERSPRNVVGVGREGELFNWATAAAAAKTTAVTGEGIVGVNMEEKAIVF 313

Query: 313 GWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
                 G+ G+FM E QNGK ER+E+P EFSGFVQSACYT I
Sbjct: 314 -----MGRNGVFMGECQNGKLERIELPEEFSGFVQSACYTHI 348

BLAST of MS022954 vs. NCBI nr
Match: XP_008443007.1 (PREDICTED: F-box/kelch-repeat protein At2g44130-like [Cucumis melo] >KAA0043760.1 F-box/kelch-repeat protein [Cucumis melo var. makuwa] >TYK25373.1 F-box/kelch-repeat protein [Cucumis melo var. makuwa])

HSP 1 Score: 471.9 bits (1213), Expect = 4.9e-129
Identity = 243/346 (70.23%), Postives = 271/346 (78.32%), Query Frame = 0

Query: 9   MTMA-ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKA 68
           M MA ELIPG+PEEIALECLTRSH+TTHR+AARV RRWR+LFL   FY LR  SGRTHKA
Sbjct: 2   MDMADELIPGLPEEIALECLTRSHFTTHRLAARVSRRWRRLFLSRHFYNLRKLSGRTHKA 61

Query: 69  VSLIQSLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSK 128
           V  +QSL      D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   
Sbjct: 62  VFAVQSLPLPVS-DEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVD 121

Query: 129 GKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDE 188
           GKL VIGGWDP +YRPVEDVFVY+FA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHDE
Sbjct: 122 GKLAVIGGWDPVSYRPVEDVFVYDFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDE 181

Query: 189 GKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEV 248
           GKNAAATAW Y++R DEWRELPAMGA RDECEAVAIGSEIWVVSGY TENQG F  +AEV
Sbjct: 182 GKNAAATAWVYNIRNDEWRELPAMGAGRDECEAVAIGSEIWVVSGYETENQGDFERTAEV 241

Query: 249 FDTKTGTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAAATAK---EGMVGVELGER- 308
           ++TKTG WRRV+ AW  ER PR VVGV   G ++NWA  AA T     EG+VGV + E+ 
Sbjct: 242 YETKTGKWRRVENAWCEERSPRNVVGVGREGELFNWATVAAKTTAVEGEGIVGVNMEEKG 301

Query: 309 AMVFGWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
           AMVF      G+ G+FM EGQ GK ER+E+P EFSGFVQSA YT I
Sbjct: 302 AMVF-----MGRNGVFMGEGQYGKLERIELPEEFSGFVQSASYTHI 340

BLAST of MS022954 vs. NCBI nr
Match: XP_023528326.1 (F-box/kelch-repeat protein At2g44130-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 470.3 bits (1209), Expect = 1.4e-128
Identity = 235/340 (69.12%), Postives = 267/340 (78.53%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIAL+CL RSH+TTHR+AARVCRRWR LFL  DFY LR  S RTHKAV  +Q
Sbjct: 4   ELIPGLPEEIALDCLIRSHFTTHRIAARVCRRWRGLFLSRDFYNLRRISDRTHKAVFAVQ 63

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL   AG DG K +T+  A+G+S FDPA+G WT I+PIGKYPNGLPLFCR+I   GKLVV
Sbjct: 64  SLLLPAG-DGDK-STAPPAFGLSAFDPASGKWTWIKPIGKYPNGLPLFCRLIGVDGKLVV 123

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDPA+YRPVEDVFVY+ A E+WRQGK MP  RSFF A   GGEIFVAGGHDE KNAA
Sbjct: 124 IGGWDPASYRPVEDVFVYDIAAEKWRQGKEMPATRSFFSAVECGGEIFVAGGHDEWKNAA 183

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           +TAWAY+++ DEWRELPAM   RDECEAVAIGS+IWVVSGY TENQG F  SAE+F+T+T
Sbjct: 184 STAWAYNIKNDEWRELPAMSCGRDECEAVAIGSDIWVVSGYRTENQGNFEASAEIFNTET 243

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWA---EAAAATAKEGMVGVELGERAMVFGW 312
             WRRVD AW+ ER PRGVVGV   G +++WA   E   A   EG+VGV +  +AMVF  
Sbjct: 244 EEWRRVDSAWREERSPRGVVGVSREGELFSWAAAVETTTAVTAEGVVGVNMEGKAMVF-- 303

Query: 313 EYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
               G++GIF REGQN KFERMEVP EF GFVQSACYTEI
Sbjct: 304 ---VGRHGIFTREGQNSKFERMEVPEEFRGFVQSACYTEI 336

BLAST of MS022954 vs. ExPASy Swiss-Prot
Match: O80582 (F-box/kelch-repeat protein At2g44130 OS=Arabidopsis thaliana OX=3702 GN=At2g44130 PE=2 SV=2)

HSP 1 Score: 192.2 bits (487), Expect = 9.9e-48
Identity = 108/283 (38.16%), Postives = 154/283 (54.42%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+P E+ALECL R  +        VCR WR L     F + R + G+T   + L+Q
Sbjct: 18  ELIPGLPSELALECLVRVPFQFQSAMRSVCRSWRSLLSDSSFIQERRRCGKTELLLCLVQ 77

Query: 73  SLSAQAGP----------DGAKAATSAN-------AYGVSVFDPAAGNWTRIEPIGKYP- 132
            L+               D  K+   ++        +G+SV++ A   W R+     +P 
Sbjct: 78  PLTPPIPASKSVDETLMVDEKKSEDESHPRVFCTPRFGLSVYNAAMSTWHRV----AFPE 137

Query: 133 -NGLPLFCR--IISSKGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFG 192
              +PLFC   ++   GK+++IGGWDP T +P  DV+V EFA  +WR+G  M E RSFF 
Sbjct: 138 EEQIPLFCECVVLQDAGKILLIGGWDPETLQPTRDVYVLEFAGRKWRRGAPMKESRSFFA 197

Query: 193 -AAAHGGEIFVAGGHDEGKNAAATAWAYDVRQDEWRELPAMGAARDECE--AVAIGSEIW 252
            A+    +++VAGGHD+ KNA  +A  YDV +DEW  +  M   RDEC+  AV +G    
Sbjct: 198 CASVSPTKVYVAGGHDDQKNALRSAEVYDVEKDEWSSVTPMTEGRDECQGFAVGMGLRFC 257

Query: 253 VVSGYVTENQGAFVDSAEVFDTKTGTWRRVDGAWK-GERGPRG 271
           V+SGY TE+QG F    E++D  T +W R+D  W+  +  PRG
Sbjct: 258 VLSGYGTESQGRFRSDGEIYDPATDSWSRIDNVWRFPDTSPRG 296

BLAST of MS022954 vs. ExPASy Swiss-Prot
Match: Q9LMR5 (F-box/kelch-repeat protein At1g15670 OS=Arabidopsis thaliana OX=3702 GN=At1g15670 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.3e-47
Identity = 108/251 (43.03%), Postives = 140/251 (55.78%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIP +PE +A ECL RS Y    + A VC+ W++     DF++ R  SG + + V L  
Sbjct: 2   ELIPDLPETVAYECLLRSSYKQFPLMASVCKLWQREISLSDFFRHRKASGHSQELVVL-- 61

Query: 73  SLSAQAGPDGAKAATSAN------AYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISS 132
              +QA  D  K   S N       Y +SV +   G  + + P+  + NGLPLFCR++S 
Sbjct: 62  ---SQARVDPVKELVSGNKTIPTPVYRISVLELGTGLRSELPPVPGHSNGLPLFCRLVSV 121

Query: 133 KGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMP-ERRSFFGAAAHG-GEIFVAGG 192
              LVV+ G DP T+R  + VFV+ F T  WR GK+MP   RSFF  A+     +FVAGG
Sbjct: 122 GSDLVVLCGLDPVTWRTSDSVFVFSFLTSTWRVGKSMPGGPRSFFACASDSQRNVFVAGG 181

Query: 193 HDEGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDS 252
           HDE KNA  +A  YDV +D W  LP MG  RDEC A+    +  V+ GY TE QG F  +
Sbjct: 182 HDEDKNAMMSALVYDVAEDRWAFLPDMGRERDECTAIFHAGKFHVIGGYSTEEQGQFSKT 241

Query: 253 AEVFDTKTGTW 256
           AE FD  T  W
Sbjct: 242 AESFDVTTWRW 247

BLAST of MS022954 vs. ExPASy Swiss-Prot
Match: Q9M8L2 (F-box/kelch-repeat protein At1g80440 OS=Arabidopsis thaliana OX=3702 GN=At1g80440 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.9e-47
Identity = 103/245 (42.04%), Postives = 136/245 (55.51%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIP +P+++A ECL RS Y    V A VCR W +      F   R  S  + + + L Q
Sbjct: 2   ELIPNLPDDVARECLLRSSYQQFPVIASVCRAWNREVSLSQFLHQRKASRHSQELLILSQ 61

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
              A+  P G+    +   Y +SV +  +G WT + PI     GLPLFCR++S    L+V
Sbjct: 62  ---ARVDPAGSGKIIATPEYRISVLESGSGLWTELPPIPGQTKGLPLFCRLVSVGSDLIV 121

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMP-ERRSFFGAAAHGGE-IFVAGGHDEGKN 192
           +GG DP T++  + VFV+ F T +WR G  MP  RRSFFG A+     + VAGGH+E K 
Sbjct: 122 LGGLDPITWQAHDSVFVFSFLTSKWRVGATMPGVRRSFFGCASDSDRTVLVAGGHNEEKC 181

Query: 193 AAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDT 252
           A  +A  YDV +D+W  LP M   RDEC+AV       V+ GY TE QG F  +AE FD 
Sbjct: 182 ALTSAMVYDVSEDKWTFLPDMARERDECKAVFHAGRFHVIGGYATEEQGQFSKTAESFDV 241

Query: 253 KTGTW 256
            T  W
Sbjct: 242 STWEW 243

BLAST of MS022954 vs. ExPASy Swiss-Prot
Match: Q9M1Y1 (F-box/kelch-repeat protein SKIP20 OS=Arabidopsis thaliana OX=3702 GN=SKIP20 PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 6.6e-44
Identity = 105/294 (35.71%), Postives = 152/294 (51.70%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           +LIPG+PEE+A+ECL R  +  H     VCR W+ +     F K R   G+    + L+Q
Sbjct: 14  DLIPGLPEELAIECLVRVPFQFHSSIKSVCRSWKCVISSRSFIKERIGFGKAESLLCLVQ 73

Query: 73  SLSAQAGP---DGAKAA----------------------TSANAYGVSVFDPAAGNWTRI 132
            L++   P   +G + +                      T    YG+SV++     W R+
Sbjct: 74  PLTSPPSPAMMEGGEMSQKKKEEEEGESQMTQQLLQPRITGTPLYGLSVYNATLDTWHRV 133

Query: 133 EPIGKYPNGLPLFCR--IISSKGKLVVIGGWDPATYRPVEDVFVYEF-----ATERWRQG 192
                 P  +PLFC    I   GK+++IGGWDP T +PV DVFV +F     +  R+R+G
Sbjct: 134 ----AIPERIPLFCECVAIQDAGKVLLIGGWDPETLQPVRDVFVLDFFAGEGSGRRFRRG 193

Query: 193 KAMPERRSFFGAAAHGG-EIFVAGGHDEGKNAAATAWAYDVRQDEWRELPAMGAARDECE 252
           + M   RSFF  A+ G  +++VAGGHD+ KNA  +A  YDV +DEW  LP M   RDEC 
Sbjct: 194 RPMSAARSFFACASVGSTKVYVAGGHDDQKNALRSAEVYDVEKDEWSMLPPMTEGRDECH 253

Query: 253 AVAIGSE--IWVVSGYVTENQGAFVDSAEVFDTKTGTWRRVDGAWK-GERGPRG 271
             ++ ++    V+SGY TE QG F    E++D  T +W  ++  W   +  PRG
Sbjct: 254 GFSMATDPGFCVLSGYGTETQGQFRSDGEIYDPITNSWSTIENVWPFPDLSPRG 303

BLAST of MS022954 vs. ExPASy Swiss-Prot
Match: Q93W93 (F-box/kelch-repeat protein At1g55270 OS=Arabidopsis thaliana OX=3702 GN=At1g55270 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 5.8e-16
Identity = 70/265 (26.42%), Postives = 111/265 (41.89%), Query Frame = 0

Query: 7   KTMTMAELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHK 66
           +T     L+PG+P+++A+ CL R     HR    VC+RW +L  G  FY  R   G + +
Sbjct: 71  RTRIQPPLLPGLPDDLAVACLIRVPRAEHRKLRLVCKRWYRLASGNFFYSQRKLLGMSEE 130

Query: 67  AVSLIQSLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGK-YPNGLPLFCRIIS 126
            V +      +   DG            + FDP +  W  + P+ + Y   +   C ++S
Sbjct: 131 WVYVF-----KRDRDG--------KISWNTFDPISQLWQPLPPVPREYSEAVGFGCAVLS 190

Query: 127 SKGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGH 186
               L + GG DP     +  V  Y   T +W +   M  +R FFG       ++VAGG 
Sbjct: 191 G-CHLYLFGGKDPLR-GSMRRVIFYNARTNKWHRAPDMLRKRHFFGCCVINNCLYVAGGE 250

Query: 187 DEG-KNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDS 246
            EG +    +A  YD  ++ W  +  M  A      V    + W + G  +      +  
Sbjct: 251 CEGIQRTLRSAEVYDPNKNRWSFIADMSTAMVPLIGVVYDKK-WFLKGLGSHQ----LVM 310

Query: 247 AEVFDTKTGTWRRV-DGAWKGERGP 269
           +E +D +  +W  V DG   G R P
Sbjct: 311 SEAYDPEVNSWSPVSDGMVAGWRNP 315

BLAST of MS022954 vs. ExPASy TrEMBL
Match: A0A0A0LEY5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G792070 PE=4 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 4.8e-130
Identity = 240/342 (70.18%), Postives = 268/342 (78.36%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIALECLTRSH+TTHRVAARV RRW +LFL   FY LR  SGRTHKAV  +Q
Sbjct: 14  ELIPGLPEEIALECLTRSHFTTHRVAARVSRRWHRLFLSRHFYNLRKLSGRTHKAVFAVQ 73

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL  Q   D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   GKL V
Sbjct: 74  SL-LQPVSDEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVDGKLAV 133

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDP +YRPVEDVFVYEFA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHDEGKNAA
Sbjct: 134 IGGWDPVSYRPVEDVFVYEFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDEGKNAA 193

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           A+AW Y++R DEWRELPAM   RDECEAVAIGSEIWVVSGY TENQG F  +AEV++TKT
Sbjct: 194 ASAWVYNIRNDEWRELPAMSRGRDECEAVAIGSEIWVVSGYETENQGNFERTAEVYETKT 253

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAA-----ATAKEGMVGVELGERAMVF 312
           G WRRV+ AW  ER PR VVGV   G ++NWA AAA     A   EG+VGV + E+A+VF
Sbjct: 254 GKWRRVESAWCEERSPRNVVGVGREGELFNWATAAAAAKTTAVTGEGIVGVNMEEKAIVF 313

Query: 313 GWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
                 G+ G+FM E QNGK ER+E+P EFSGFVQSACYT I
Sbjct: 314 -----MGRNGVFMGECQNGKLERIELPEEFSGFVQSACYTHI 348

BLAST of MS022954 vs. ExPASy TrEMBL
Match: A0A5D3DP02 (F-box/kelch-repeat protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005130 PE=4 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.4e-129
Identity = 243/346 (70.23%), Postives = 271/346 (78.32%), Query Frame = 0

Query: 9   MTMA-ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKA 68
           M MA ELIPG+PEEIALECLTRSH+TTHR+AARV RRWR+LFL   FY LR  SGRTHKA
Sbjct: 2   MDMADELIPGLPEEIALECLTRSHFTTHRLAARVSRRWRRLFLSRHFYNLRKLSGRTHKA 61

Query: 69  VSLIQSLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSK 128
           V  +QSL      D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   
Sbjct: 62  VFAVQSLPLPVS-DEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVD 121

Query: 129 GKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDE 188
           GKL VIGGWDP +YRPVEDVFVY+FA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHDE
Sbjct: 122 GKLAVIGGWDPVSYRPVEDVFVYDFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDE 181

Query: 189 GKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEV 248
           GKNAAATAW Y++R DEWRELPAMGA RDECEAVAIGSEIWVVSGY TENQG F  +AEV
Sbjct: 182 GKNAAATAWVYNIRNDEWRELPAMGAGRDECEAVAIGSEIWVVSGYETENQGDFERTAEV 241

Query: 249 FDTKTGTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAAATAK---EGMVGVELGER- 308
           ++TKTG WRRV+ AW  ER PR VVGV   G ++NWA  AA T     EG+VGV + E+ 
Sbjct: 242 YETKTGKWRRVENAWCEERSPRNVVGVGREGELFNWATVAAKTTAVEGEGIVGVNMEEKG 301

Query: 309 AMVFGWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
           AMVF      G+ G+FM EGQ GK ER+E+P EFSGFVQSA YT I
Sbjct: 302 AMVF-----MGRNGVFMGEGQYGKLERIELPEEFSGFVQSASYTHI 340

BLAST of MS022954 vs. ExPASy TrEMBL
Match: A0A1S3B7S9 (F-box/kelch-repeat protein At2g44130-like OS=Cucumis melo OX=3656 GN=LOC103486736 PE=4 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.4e-129
Identity = 243/346 (70.23%), Postives = 271/346 (78.32%), Query Frame = 0

Query: 9   MTMA-ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKA 68
           M MA ELIPG+PEEIALECLTRSH+TTHR+AARV RRWR+LFL   FY LR  SGRTHKA
Sbjct: 2   MDMADELIPGLPEEIALECLTRSHFTTHRLAARVSRRWRRLFLSRHFYNLRKLSGRTHKA 61

Query: 69  VSLIQSLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSK 128
           V  +QSL      D AK+A    A+GVS FDPA GNWTRI+PI KYPNGLPLFCRII   
Sbjct: 62  VFAVQSLPLPVS-DEAKSAAPI-AFGVSAFDPATGNWTRIKPIEKYPNGLPLFCRIIGVD 121

Query: 129 GKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDE 188
           GKL VIGGWDP +YRPVEDVFVY+FA E+WRQGK MPE+RSFFGA  +GGEIFVAGGHDE
Sbjct: 122 GKLAVIGGWDPVSYRPVEDVFVYDFAAEKWRQGKGMPEKRSFFGATEYGGEIFVAGGHDE 181

Query: 189 GKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEV 248
           GKNAAATAW Y++R DEWRELPAMGA RDECEAVAIGSEIWVVSGY TENQG F  +AEV
Sbjct: 182 GKNAAATAWVYNIRNDEWRELPAMGAGRDECEAVAIGSEIWVVSGYETENQGDFERTAEV 241

Query: 249 FDTKTGTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAAATAK---EGMVGVELGER- 308
           ++TKTG WRRV+ AW  ER PR VVGV   G ++NWA  AA T     EG+VGV + E+ 
Sbjct: 242 YETKTGKWRRVENAWCEERSPRNVVGVGREGELFNWATVAAKTTAVEGEGIVGVNMEEKG 301

Query: 309 AMVFGWEYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
           AMVF      G+ G+FM EGQ GK ER+E+P EFSGFVQSA YT I
Sbjct: 302 AMVF-----MGRNGVFMGEGQYGKLERIELPEEFSGFVQSASYTHI 340

BLAST of MS022954 vs. ExPASy TrEMBL
Match: A0A6J1J0Y8 (F-box/kelch-repeat protein At2g44130-like OS=Cucurbita maxima OX=3661 GN=LOC111481691 PE=4 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 2.2e-127
Identity = 233/340 (68.53%), Postives = 267/340 (78.53%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIAL+CL RSH+TTHR+AARVCRRWR LFL  DF+ LR  S RTHKAV  +Q
Sbjct: 4   ELIPGLPEEIALDCLIRSHFTTHRIAARVCRRWRGLFLSRDFHNLRRISDRTHKAVFAVQ 63

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL   AG DG K +T+  A+G+S FDPA G WT I+PI KY NGLPLFCR+I   GKLVV
Sbjct: 64  SLLVPAG-DGDK-STAPLAFGLSAFDPATGKWTWIKPIEKYSNGLPLFCRLIGVDGKLVV 123

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDPA+YRPVEDVFVY+ A E+WRQGK MP  RSFF A   GGEIFVAGGHDE KNAA
Sbjct: 124 IGGWDPASYRPVEDVFVYDMAAEKWRQGKGMPATRSFFSAVECGGEIFVAGGHDEWKNAA 183

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           +TAWAY+++ DEWRELPAM   RDECEAVAIGS+IWVVSGY TENQG F  +AE+FDT+T
Sbjct: 184 STAWAYNIKNDEWRELPAMSCGRDECEAVAIGSDIWVVSGYRTENQGNFEATAEIFDTET 243

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWAEAAAAT---AKEGMVGVELGERAMVFGW 312
             WRRVD AW+ ER PRGVVGV   GG+++WA A AAT     E +VGV +  +AMVF  
Sbjct: 244 EKWRRVDSAWREERSPRGVVGVSREGGLFSWAAAVAATTAVTAEEVVGVNMEGKAMVF-- 303

Query: 313 EYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
               G++GIF REGQN KFERMEVP +F+GFVQSACYTEI
Sbjct: 304 ---VGRHGIFTREGQNSKFERMEVPEDFTGFVQSACYTEI 336

BLAST of MS022954 vs. ExPASy TrEMBL
Match: A0A6J1F4W9 (F-box/kelch-repeat protein At2g44130-like OS=Cucurbita moschata OX=3662 GN=LOC111442196 PE=4 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 6.1e-125
Identity = 228/340 (67.06%), Postives = 262/340 (77.06%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+PEEIAL+CL RSH+TTHR+AARVCRRWR LFL  DFY LR  S RTHK V  +Q
Sbjct: 4   ELIPGLPEEIALDCLIRSHFTTHRIAARVCRRWRGLFLSRDFYNLRRISDRTHKVVFAVQ 63

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
           SL   AG DG K +T+  A+G+S FDPA G WT I+PI KYPN LPLFCR+I   GKLVV
Sbjct: 64  SLLVPAG-DGDK-STAPPAFGLSAFDPATGKWTWIKPIEKYPNRLPLFCRLIGVDGKLVV 123

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFVAGGHDEGKNAA 192
           IGGWDPA+YRPVE+VFVY+ A E+WRQGK MP  RSFF A   GGEIFVAGGHDE KNAA
Sbjct: 124 IGGWDPASYRPVEEVFVYDIAAEKWRQGKGMPATRSFFSAVEFGGEIFVAGGHDEWKNAA 183

Query: 193 ATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDTKT 252
           +TAW Y+++ DEWRELPAM   RDECEAVAIGS+IWVVSGY TENQG F  +AE+F+T+T
Sbjct: 184 STAWVYNIKNDEWRELPAMSYGRDECEAVAIGSDIWVVSGYRTENQGNFEATAEIFNTET 243

Query: 253 GTWRRVDGAWKGERGPRGVVGVRGGGGIYNWA---EAAAATAKEGMVGVELGERAMVFGW 312
             WRRVD AW+ ER PRGVVGV   G +++WA   E   A   EG+VGV +  +AMVF  
Sbjct: 244 EEWRRVDSAWREERSPRGVVGVSREGELFSWAAAVETTTAVTAEGVVGVTMEGKAMVF-- 303

Query: 313 EYLGGKYGIFMREGQNGKFERMEVPSEFSGFVQSACYTEI 350
               G++GIF REG N KFERMEVP EF+GFVQSACYTEI
Sbjct: 304 ---VGRHGIFTREGHNSKFERMEVPEEFTGFVQSACYTEI 336

BLAST of MS022954 vs. TAIR 10
Match: AT2G44130.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 192.2 bits (487), Expect = 7.0e-49
Identity = 108/283 (38.16%), Postives = 154/283 (54.42%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIPG+P E+ALECL R  +        VCR WR L     F + R + G+T   + L+Q
Sbjct: 18  ELIPGLPSELALECLVRVPFQFQSAMRSVCRSWRSLLSDSSFIQERRRCGKTELLLCLVQ 77

Query: 73  SLSAQAGP----------DGAKAATSAN-------AYGVSVFDPAAGNWTRIEPIGKYP- 132
            L+               D  K+   ++        +G+SV++ A   W R+     +P 
Sbjct: 78  PLTPPIPASKSVDETLMVDEKKSEDESHPRVFCTPRFGLSVYNAAMSTWHRV----AFPE 137

Query: 133 -NGLPLFCR--IISSKGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMPERRSFFG 192
              +PLFC   ++   GK+++IGGWDP T +P  DV+V EFA  +WR+G  M E RSFF 
Sbjct: 138 EEQIPLFCECVVLQDAGKILLIGGWDPETLQPTRDVYVLEFAGRKWRRGAPMKESRSFFA 197

Query: 193 -AAAHGGEIFVAGGHDEGKNAAATAWAYDVRQDEWRELPAMGAARDECE--AVAIGSEIW 252
            A+    +++VAGGHD+ KNA  +A  YDV +DEW  +  M   RDEC+  AV +G    
Sbjct: 198 CASVSPTKVYVAGGHDDQKNALRSAEVYDVEKDEWSSVTPMTEGRDECQGFAVGMGLRFC 257

Query: 253 VVSGYVTENQGAFVDSAEVFDTKTGTWRRVDGAWK-GERGPRG 271
           V+SGY TE+QG F    E++D  T +W R+D  W+  +  PRG
Sbjct: 258 VLSGYGTESQGRFRSDGEIYDPATDSWSRIDNVWRFPDTSPRG 296

BLAST of MS022954 vs. TAIR 10
Match: AT1G15670.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 191.8 bits (486), Expect = 9.2e-49
Identity = 108/251 (43.03%), Postives = 140/251 (55.78%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIP +PE +A ECL RS Y    + A VC+ W++     DF++ R  SG + + V L  
Sbjct: 2   ELIPDLPETVAYECLLRSSYKQFPLMASVCKLWQREISLSDFFRHRKASGHSQELVVL-- 61

Query: 73  SLSAQAGPDGAKAATSAN------AYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISS 132
              +QA  D  K   S N       Y +SV +   G  + + P+  + NGLPLFCR++S 
Sbjct: 62  ---SQARVDPVKELVSGNKTIPTPVYRISVLELGTGLRSELPPVPGHSNGLPLFCRLVSV 121

Query: 133 KGKLVVIGGWDPATYRPVEDVFVYEFATERWRQGKAMP-ERRSFFGAAAHG-GEIFVAGG 192
              LVV+ G DP T+R  + VFV+ F T  WR GK+MP   RSFF  A+     +FVAGG
Sbjct: 122 GSDLVVLCGLDPVTWRTSDSVFVFSFLTSTWRVGKSMPGGPRSFFACASDSQRNVFVAGG 181

Query: 193 HDEGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDS 252
           HDE KNA  +A  YDV +D W  LP MG  RDEC A+    +  V+ GY TE QG F  +
Sbjct: 182 HDEDKNAMMSALVYDVAEDRWAFLPDMGRERDECTAIFHAGKFHVIGGYSTEEQGQFSKT 241

Query: 253 AEVFDTKTGTW 256
           AE FD  T  W
Sbjct: 242 AESFDVTTWRW 247

BLAST of MS022954 vs. TAIR 10
Match: AT1G80440.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 190.7 bits (483), Expect = 2.0e-48
Identity = 103/245 (42.04%), Postives = 136/245 (55.51%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           ELIP +P+++A ECL RS Y    V A VCR W +      F   R  S  + + + L Q
Sbjct: 2   ELIPNLPDDVARECLLRSSYQQFPVIASVCRAWNREVSLSQFLHQRKASRHSQELLILSQ 61

Query: 73  SLSAQAGPDGAKAATSANAYGVSVFDPAAGNWTRIEPIGKYPNGLPLFCRIISSKGKLVV 132
              A+  P G+    +   Y +SV +  +G WT + PI     GLPLFCR++S    L+V
Sbjct: 62  ---ARVDPAGSGKIIATPEYRISVLESGSGLWTELPPIPGQTKGLPLFCRLVSVGSDLIV 121

Query: 133 IGGWDPATYRPVEDVFVYEFATERWRQGKAMP-ERRSFFGAAAHGGE-IFVAGGHDEGKN 192
           +GG DP T++  + VFV+ F T +WR G  MP  RRSFFG A+     + VAGGH+E K 
Sbjct: 122 LGGLDPITWQAHDSVFVFSFLTSKWRVGATMPGVRRSFFGCASDSDRTVLVAGGHNEEKC 181

Query: 193 AAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVSGYVTENQGAFVDSAEVFDT 252
           A  +A  YDV +D+W  LP M   RDEC+AV       V+ GY TE QG F  +AE FD 
Sbjct: 182 ALTSAMVYDVSEDKWTFLPDMARERDECKAVFHAGRFHVIGGYATEEQGQFSKTAESFDV 241

Query: 253 KTGTW 256
            T  W
Sbjct: 242 STWEW 243

BLAST of MS022954 vs. TAIR 10
Match: AT3G59940.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 4.7e-45
Identity = 105/294 (35.71%), Postives = 152/294 (51.70%), Query Frame = 0

Query: 13  ELIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQ 72
           +LIPG+PEE+A+ECL R  +  H     VCR W+ +     F K R   G+    + L+Q
Sbjct: 14  DLIPGLPEELAIECLVRVPFQFHSSIKSVCRSWKCVISSRSFIKERIGFGKAESLLCLVQ 73

Query: 73  SLSAQAGP---DGAKAA----------------------TSANAYGVSVFDPAAGNWTRI 132
            L++   P   +G + +                      T    YG+SV++     W R+
Sbjct: 74  PLTSPPSPAMMEGGEMSQKKKEEEEGESQMTQQLLQPRITGTPLYGLSVYNATLDTWHRV 133

Query: 133 EPIGKYPNGLPLFCR--IISSKGKLVVIGGWDPATYRPVEDVFVYEF-----ATERWRQG 192
                 P  +PLFC    I   GK+++IGGWDP T +PV DVFV +F     +  R+R+G
Sbjct: 134 ----AIPERIPLFCECVAIQDAGKVLLIGGWDPETLQPVRDVFVLDFFAGEGSGRRFRRG 193

Query: 193 KAMPERRSFFGAAAHGG-EIFVAGGHDEGKNAAATAWAYDVRQDEWRELPAMGAARDECE 252
           + M   RSFF  A+ G  +++VAGGHD+ KNA  +A  YDV +DEW  LP M   RDEC 
Sbjct: 194 RPMSAARSFFACASVGSTKVYVAGGHDDQKNALRSAEVYDVEKDEWSMLPPMTEGRDECH 253

Query: 253 AVAIGSE--IWVVSGYVTENQGAFVDSAEVFDTKTGTWRRVDGAWK-GERGPRG 271
             ++ ++    V+SGY TE QG F    E++D  T +W  ++  W   +  PRG
Sbjct: 254 GFSMATDPGFCVLSGYGTETQGQFRSDGEIYDPITNSWSTIENVWPFPDLSPRG 303

BLAST of MS022954 vs. TAIR 10
Match: AT1G16250.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 4.1e-17
Identity = 75/296 (25.34%), Postives = 128/296 (43.24%), Query Frame = 0

Query: 14  LIPGVPEEIALECLTRSHYTTHRVAARVCRRWRQLFLGPDFYKLRHKSGRTHKAVSLIQS 73
           +IPG+P+++AL C+ +  +  H V   V R WR L  G D+   + ++G +   + ++  
Sbjct: 8   IIPGLPDDLALRCIAKLSHGYHGVLECVSRGWRDLVRGADYSCYKARNGWSGSWLFVL-- 67

Query: 74  LSAQAGPDGAKAATSANAYGVSVFDPAAGNW-----TRIEPIGKYPNGLPLFCRIISSKG 133
                        T  +      +DP A  W     TR    G + +G    C    S  
Sbjct: 68  -------------TERSKNQWVAYDPEADRWHPLPRTRAVQDGWHHSGFACVC---VSNC 127

Query: 134 KLVVIGGWDPAT-----YRPV--EDVFVYEFATERWRQGKAMPERRSFFGAAAHGGEIFV 193
            LV+ G + P+       +PV  +DV  ++   ++W+   +M   R+ F   +  G+++V
Sbjct: 128 LLVIGGCYAPSVSSFPHQKPVVTKDVMRFDPFKKQWKMVASMRTPRTHFACTSVSGKVYV 187

Query: 194 AGGHD-EGKNAAATAWAYDVRQDEWRELPAMGAARDECEAVAIGSEIWVVS---GYVTEN 253
           AGG +        +A  YD   D W ELPAM   + +C  ++      V+S   G+  +N
Sbjct: 188 AGGRNLTHSRGIPSAEVYDPVADRWEELPAMPRPQMDCSGLSYRGCFHVLSDQVGFAEQN 247

Query: 254 QGAFVDSAEVFDTKTGTWRRVDGAWKGERGPRGVVGVRGGGGIY---NWAEAAAAT 291
                 S+EVF+ +  TW  V+  W   R  +  V V     +Y   +W E+   T
Sbjct: 248 ------SSEVFNPRDMTWSTVEDVWPFSRAMQFAVQVMKNDRVYTIVDWGESLIKT 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038904459.18.9e-13974.49F-box/kelch-repeat protein At2g44130-like [Benincasa hispida][more]
XP_004136552.39.9e-13070.18F-box/kelch-repeat protein At2g44130 [Cucumis sativus][more]
KGN59267.19.9e-13070.18hypothetical protein Csa_002357 [Cucumis sativus][more]
XP_008443007.14.9e-12970.23PREDICTED: F-box/kelch-repeat protein At2g44130-like [Cucumis melo] >KAA0043760.... [more]
XP_023528326.11.4e-12869.12F-box/kelch-repeat protein At2g44130-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O805829.9e-4838.16F-box/kelch-repeat protein At2g44130 OS=Arabidopsis thaliana OX=3702 GN=At2g4413... [more]
Q9LMR51.3e-4743.03F-box/kelch-repeat protein At1g15670 OS=Arabidopsis thaliana OX=3702 GN=At1g1567... [more]
Q9M8L22.9e-4742.04F-box/kelch-repeat protein At1g80440 OS=Arabidopsis thaliana OX=3702 GN=At1g8044... [more]
Q9M1Y16.6e-4435.71F-box/kelch-repeat protein SKIP20 OS=Arabidopsis thaliana OX=3702 GN=SKIP20 PE=1... [more]
Q93W935.8e-1626.42F-box/kelch-repeat protein At1g55270 OS=Arabidopsis thaliana OX=3702 GN=At1g5527... [more]
Match NameE-valueIdentityDescription
A0A0A0LEY54.8e-13070.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G792070 PE=4 SV=1[more]
A0A5D3DP022.4e-12970.23F-box/kelch-repeat protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3B7S92.4e-12970.23F-box/kelch-repeat protein At2g44130-like OS=Cucumis melo OX=3656 GN=LOC10348673... [more]
A0A6J1J0Y82.2e-12768.53F-box/kelch-repeat protein At2g44130-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1F4W96.1e-12567.06F-box/kelch-repeat protein At2g44130-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT2G44130.17.0e-4938.16Galactose oxidase/kelch repeat superfamily protein [more]
AT1G15670.19.2e-4943.03Galactose oxidase/kelch repeat superfamily protein [more]
AT1G80440.12.0e-4842.04Galactose oxidase/kelch repeat superfamily protein [more]
AT3G59940.14.7e-4535.71Galactose oxidase/kelch repeat superfamily protein [more]
AT1G16250.14.1e-1725.34Galactose oxidase/kelch repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006652Kelch repeat type 1SMARTSM00612kelc_smartcoord: 226..276
e-value: 18.0
score: 0.6
coord: 178..225
e-value: 2.7E-5
score: 33.6
coord: 129..177
e-value: 8.6E-9
score: 45.2
IPR006652Kelch repeat type 1PFAMPF01344Kelch_1coord: 166..211
e-value: 1.1E-12
score: 47.3
coord: 125..164
e-value: 7.6E-7
score: 28.6
coord: 216..258
e-value: 2.0E-5
score: 24.1
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 63..311
e-value: 9.0E-41
score: 141.7
IPR015915Kelch-type beta propellerSUPERFAMILY117281Kelch motifcoord: 92..268
IPR044595F-box/kelch-repeat protein KMD1/2-likePANTHERPTHR46407OS02G0208700 PROTEINcoord: 10..349
NoneNo IPR availablePANTHERPTHR46407:SF6F-BOX/KELCH-REPEAT PLANT-LIKE PROTEINcoord: 10..349
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 15..58

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS022954.1MS022954.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0080037 negative regulation of cytokinin-activated signaling pathway
biological_process GO:2000762 regulation of phenylpropanoid metabolic process
molecular_function GO:0005515 protein binding