MS020271.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS020271.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	actin-depolymerizing factor
Location	scaffold665: 647246 .. 648219 (+)
Sequence length	432
RNA-Seq Expression	MS020271.1
Synteny	MS020271.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TATGAATTACTTTTGCAGCAAAACACTTTATCTGCAATGGGTGTGAATGAACACACCAAGAAAACGTTTTCAGAACTACAGAGAAAGAAAATGTACCGATATGTGATCTTTAAGGTTGATGAAAAGAAAAGGGAGGTGGTGGTTGAAAAGACTGGCAGTCCTGCAGAGAGCTATGAAGATTTCACTGCCGCTCTTCCCGACAATGATTGCCGCTATGCCGTGTATGATTTTGATTTCGTGACATCTGACAACTGTCAGAAGAGCAAGATATTCTTCATCGCATGGTTAGCTTTCAACTATTATGAACTCAATCTTATCTTTCTTATTGCTATGTTAATTTGATTATACTATTGCTCATTGAATTGCCCCACAACACAAAACTTTGGATACTAAGGAAAACAATGGCTATCCTAAGGTCATAAGAGTTTTGATCCTACAACCACCGATTCTAACCTGTGACTATTGAAAATCAAACTACCATTTCTAAGCTTTATGGTTCTTCATGTTCATGGTCAAATGGTTATTCATGAAACTCATCTGGTATGCTTACTATTTTTATTAAATACCTACTTAGTCTTCTGTTAGAGTTTAGTGGTAAGAGTTCTATATGAATCTCCACTACTCTCCGAATATCATAGCATATCAAAATTCTTGTCCCTTGATATTTTTGGGCAAAACTGAAAAATCCTGTCATATTTTTACCAGAATGATTGCTTCTGTTGTTGGTGATCCAATGCACCTTTTGAATCTCTTATGCTGAAAACCATCAAAGTTATGACCAGGTGTTTTTTCTCTCTTGATCTCACACTAGATTGTCGTACTTCAGGTCTCCAGCTAGCTCACGAATCCGTGCTAAGATGCTCTATGCGACATCTAAAGACAACTTTCGGCACGAGCTTGATGGCATCCACTATGAGATTCAAGCTACTGACCCTGCAGAGATGGACCTGGAAGTGATTAGAGACCGTGCTCAA

mRNA sequence

TATGAATTACTTTTGCAGCAAAACACTTTATCTGCAATGGGTGTGAATGAACACACCAAGAAAACGTTTTCAGAACTACAGAGAAAGAAAATGTACCGATATGTGATCTTTAAGGTTGATGAAAAGAAAAGGGAGGTGGTGGTTGAAAAGACTGGCAGTCCTGCAGAGAGCTATGAAGATTTCACTGCCGCTCTTCCCGACAATGATTGCCGCTATGCCGTGTATGATTTTGATTTCGTGACATCTGACAACTGTCAGAAGAGCAAGATATTCTTCATCGCATGGTCTCCAGCTAGCTCACGAATCCGTGCTAAGATGCTCTATGCGACATCTAAAGACAACTTTCGGCACGAGCTTGATGGCATCCACTATGAGATTCAAGCTACTGACCCTGCAGAGATGGACCTGGAAGTGATTAGAGACCGTGCTCAA

Coding sequence (CDS)

TATGAATTACTTTTGCAGCAAAACACTTTATCTGCAATGGGTGTGAATGAACACACCAAGAAAACGTTTTCAGAACTACAGAGAAAGAAAATGTACCGATATGTGATCTTTAAGGTTGATGAAAAGAAAAGGGAGGTGGTGGTTGAAAAGACTGGCAGTCCTGCAGAGAGCTATGAAGATTTCACTGCCGCTCTTCCCGACAATGATTGCCGCTATGCCGTGTATGATTTTGATTTCGTGACATCTGACAACTGTCAGAAGAGCAAGATATTCTTCATCGCATGGTCTCCAGCTAGCTCACGAATCCGTGCTAAGATGCTCTATGCGACATCTAAAGACAACTTTCGGCACGAGCTTGATGGCATCCACTATGAGATTCAAGCTACTGACCCTGCAGAGATGGACCTGGAAGTGATTAGAGACCGTGCTCAA

Protein sequence

YELLLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPAEMDLEVIRDRAQ

Homology

[Close]

BLAST of MS020271.1 vs. NCBI nr
Match: XP_022145996.1 (actin-depolymerizing factor [Momordica charantia])

HSP 1 Score: 278.9 bits (712), Expect = 2.5e-71
Identity = 139/141 (98.58%), Postives = 140/141 (99.29%), Query Frame = 0


Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

[Close]

BLAST of MS020271.1 vs. NCBI nr
Match: TYK20532.1 (actin-depolymerizing factor [Cucumis melo var. makuwa])

HSP 1 Score: 275.8 bits (704), Expect = 2.1e-70
Identity = 136/141 (96.45%), Postives = 140/141 (99.29%), Query Frame = 0


Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           +L+QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESYEDFTA
Sbjct: 7   VLEQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTA 66

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 67  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 126

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 127 YEIQATDPAEMDLEVIRDRAQ 147

[Close]

BLAST of MS020271.1 vs. NCBI nr
Match: XP_038888227.1 (actin-depolymerizing factor [Benincasa hispida])

HSP 1 Score: 275.4 bits (703), Expect = 2.8e-70
Identity = 137/141 (97.16%), Postives = 139/141 (98.58%), Query Frame = 0


Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K GSPAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGSPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

[Close]

BLAST of MS020271.1 vs. NCBI nr
Match: XP_008447430.1 (PREDICTED: actin-depolymerizing factor [Cucumis melo] >XP_008447431.1 PREDICTED: actin-depolymerizing factor [Cucumis melo] >XP_022930980.1 actin-depolymerizing factor-like [Cucurbita moschata] >XP_022995915.1 actin-depolymerizing factor-like [Cucurbita maxima] >XP_023533852.1 actin-depolymerizing factor-like [Cucurbita pepo subsp. pepo] >KAG6606377.1 Actin-depolymerizing factor 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036318.1 Actin-depolymerizing factor 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 274.2 bits (700), Expect = 6.2e-70
Identity = 136/141 (96.45%), Postives = 139/141 (98.58%), Query Frame = 0


Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

[Close]

BLAST of MS020271.1 vs. NCBI nr
Match: XP_004151983.1 (actin-depolymerizing factor [Cucumis sativus] >XP_011651442.1 actin-depolymerizing factor [Cucumis sativus] >KGN57913.1 hypothetical protein Csa_010146 [Cucumis sativus])

HSP 1 Score: 273.1 bits (697), Expect = 1.4e-69
Identity = 135/141 (95.74%), Postives = 139/141 (98.58%), Query Frame = 0


Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIF+VDEKKREVVV+K G+PAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

[Close]

BLAST of MS020271.1 vs. ExPASy Swiss-Prot
Match: Q8SAG3 (Actin-depolymerizing factor OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 8.7e-59
Identity = 108/136 (79.41%), Postives = 124/136 (91.18%), Query Frame = 0


Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N  S MGV +H+K TF EL+RKK++RYVIFK+DEKK+EVVVEKTG PAES+++F AALP+
Sbjct: 7   NASSGMGVADHSKNTFLELKRKKVHRYVIFKIDEKKKEVVVEKTGGPAESFDEFAAALPE 66

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           NDCRYAVYDFDFVTS+NCQKSKIFFIAWSP SSRIRAKMLYATSK+ FR ELDG+HYEIQ
Sbjct: 67  NDCRYAVYDFDFVTSENCQKSKIFFIAWSPDSSRIRAKMLYATSKERFRRELDGVHYEIQ 126

Query: 128 ATDPAEMDLEVIRDRA 144
           ATDP EMDLEV+R+RA
Sbjct: 127 ATDPTEMDLEVLRERA 142

[Close]

BLAST of MS020271.1 vs. ExPASy Swiss-Prot
Match: Q9ZSK2 (Actin-depolymerizing factor 6 OS=Arabidopsis thaliana OX=3702 GN=ADF6 PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 2.2e-54
Identity = 101/140 (72.14%), Postives = 120/140 (85.71%), Query Frame = 0


Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L + N +S MGV + +K TF ELQRKK +RYV+FK+DE K+EVVVEKTG+P ESY+DF A
Sbjct: 6   LSRPNAISGMGVADESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           +LPDNDCRYAVYDFDFVTS+NCQKSKIFF AWSP++S IRAK+LY+TSKD    EL GIH
Sbjct: 66  SLPDNDCRYAVYDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRELQGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRA 144
           YEIQATDP E+DLEV+R+RA
Sbjct: 126 YEIQATDPTEVDLEVLRERA 145

[Close]

BLAST of MS020271.1 vs. ExPASy Swiss-Prot
Match: Q2QLT8 (Actin-depolymerizing factor 11 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF11 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 7.9e-52
Identity = 95/136 (69.85%), Postives = 118/136 (86.76%), Query Frame = 0


Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N  S +GV    K+TF ELQRKK +RYVIFK+D+K +EVVVEKTGS  ES++DF  +LP+
Sbjct: 9   NASSGIGVAAECKQTFLELQRKKSHRYVIFKIDDKCKEVVVEKTGSSTESFDDFMDSLPE 68

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           +DCRYA+YDFDFVT +NCQKSKIFF+AWSP+ SRIRAKMLYATSK+ FR ELDG+HYEIQ
Sbjct: 69  SDCRYAIYDFDFVTEENCQKSKIFFVAWSPSVSRIRAKMLYATSKERFRRELDGVHYEIQ 128

Query: 128 ATDPAEMDLEVIRDRA 144
           ATDP+E+D+E++R+RA
Sbjct: 129 ATDPSELDIELLRERA 144

[Close]

BLAST of MS020271.1 vs. ExPASy Swiss-Prot
Match: Q9AY76 (Actin-depolymerizing factor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF2 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 1.5e-50
Identity = 95/136 (69.85%), Postives = 115/136 (84.56%), Query Frame = 0


Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N  S MGV    + TF ELQ KK +RYVIFK++EK+++VVVEKTG+  ESY+DF A+LP+
Sbjct: 9   NASSGMGVAPDIRDTFLELQMKKAFRYVIFKIEEKQKQVVVEKTGATTESYDDFLASLPE 68

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           NDCRYA+YDFDFVT +N QKSKIFFIAWSP++SRIRAKMLY+TSKD  + ELDG HYEIQ
Sbjct: 69  NDCRYALYDFDFVTGENVQKSKIFFIAWSPSTSRIRAKMLYSTSKDRIKQELDGFHYEIQ 128

Query: 128 ATDPAEMDLEVIRDRA 144
           ATDP E+DLEV+R+RA
Sbjct: 129 ATDPTEVDLEVLRERA 144

[Close]

BLAST of MS020271.1 vs. ExPASy Swiss-Prot
Match: Q337A5 (Actin-depolymerizing factor 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF10 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 1.8e-48
Identity = 88/129 (68.22%), Postives = 113/129 (87.60%), Query Frame = 0


Query: 15  VNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPDNDCRYAV 74
           V E +K  F EL+R+K++RYVIFK+D+++ E+VVEKTG+P ESY+DFTA+LP +DCRYAV
Sbjct: 20  VPEKSKSAFWELKRRKVHRYVIFKIDDRREEIVVEKTGAPGESYDDFTASLPADDCRYAV 79

Query: 75  YDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPAEM 134
           YD DFV+ DNC+KSKIFFI+WSP+ SRIRAK +YA S++ FRHELDG+H+EIQATDP +M
Sbjct: 80  YDLDFVSDDNCRKSKIFFISWSPSVSRIRAKTIYAVSRNQFRHELDGVHFEIQATDPDDM 139

Query: 135 DLEVIRDRA 144
           DLEV+R RA
Sbjct: 140 DLEVLRGRA 148

[Close]

BLAST of MS020271.1 vs. ExPASy TrEMBL
Match: A0A6J1CY17 (actin-depolymerizing factor OS=Momordica charantia OX=3673 GN=LOC111015308 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.2e-71
Identity = 139/141 (98.58%), Postives = 140/141 (99.29%), Query Frame = 0


Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

[Close]

BLAST of MS020271.1 vs. ExPASy TrEMBL
Match: A0A5D3DAD6 (Actin-depolymerizing factor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G001160 PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.0e-70
Identity = 136/141 (96.45%), Postives = 140/141 (99.29%), Query Frame = 0


Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           +L+QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESYEDFTA
Sbjct: 7   VLEQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTA 66

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 67  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 126

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 127 YEIQATDPAEMDLEVIRDRAQ 147

[Close]

BLAST of MS020271.1 vs. ExPASy TrEMBL
Match: A0A1S3BHG1 (actin-depolymerizing factor OS=Cucumis melo OX=3656 GN=LOC103489879 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.0e-70
Identity = 136/141 (96.45%), Postives = 139/141 (98.58%), Query Frame = 0


Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

[Close]

BLAST of MS020271.1 vs. ExPASy TrEMBL
Match: A0A6J1K588 (actin-depolymerizing factor-like OS=Cucurbita maxima OX=3661 GN=LOC111491296 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.0e-70
Identity = 136/141 (96.45%), Postives = 139/141 (98.58%), Query Frame = 0


Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

[Close]

BLAST of MS020271.1 vs. ExPASy TrEMBL
Match: A0A6J1EY99 (actin-depolymerizing factor-like OS=Cucurbita moschata OX=3662 GN=LOC111437311 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.0e-70
Identity = 136/141 (96.45%), Postives = 139/141 (98.58%), Query Frame = 0


Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

[Close]

BLAST of MS020271.1 vs. TAIR 10
Match: AT2G31200.1 (actin depolymerizing factor 6 )

HSP 1 Score: 213.0 bits (541), Expect = 1.6e-55
Identity = 101/140 (72.14%), Postives = 120/140 (85.71%), Query Frame = 0


Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L + N +S MGV + +K TF ELQRKK +RYV+FK+DE K+EVVVEKTG+P ESY+DF A
Sbjct: 6   LSRPNAISGMGVADESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           +LPDNDCRYAVYDFDFVTS+NCQKSKIFF AWSP++S IRAK+LY+TSKD    EL GIH
Sbjct: 66  SLPDNDCRYAVYDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRELQGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRA 144
           YEIQATDP E+DLEV+R+RA
Sbjct: 126 YEIQATDPTEVDLEVLRERA 145

[Close]

BLAST of MS020271.1 vs. TAIR 10
Match: AT4G00680.1 (actin depolymerizing factor 8 )

HSP 1 Score: 184.1 bits (466), Expect = 7.9e-47
Identity = 81/135 (60.00%), Postives = 112/135 (82.96%), Query Frame = 0


Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N+ S M VN+  K  F EL+ K+ YR+++FK+DEK ++V +EK G+P E+Y+DFT+++PD
Sbjct: 3   NSASGMHVNDECKIKFLELKAKRTYRFIVFKIDEKAQQVQIEKLGNPEETYDDFTSSIPD 62

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           ++CRYAVYDFDF T DNCQKSKIFFIAWSP +SR+R+KMLYA+SKD F+ E++GI  E+Q
Sbjct: 63  DECRYAVYDFDFTTEDNCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKREMEGIQVELQ 122

Query: 128 ATDPAEMDLEVIRDR 143
           ATDP+EM L++I+ R
Sbjct: 123 ATDPSEMSLDIIKGR 137

[Close]

BLAST of MS020271.1 vs. TAIR 10
Match: AT1G01750.1 (actin depolymerizing factor 11 )

HSP 1 Score: 180.3 bits (456), Expect = 1.1e-45
Identity = 79/135 (58.52%), Postives = 112/135 (82.96%), Query Frame = 0


Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N+ S M V++  K  F EL+ K+ YR+++FK+DEK ++V+++K G+P E+YEDFT ++P+
Sbjct: 3   NSASGMHVSDECKLKFLELKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSIPE 62

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           ++CRYAVYD+DF T +NCQKSKIFFIAWSP +SR+R+KMLYA+SKD F+ ELDGI  E+Q
Sbjct: 63  DECRYAVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVELQ 122

Query: 128 ATDPAEMDLEVIRDR 143
           ATDP+EM L++I+ R
Sbjct: 123 ATDPSEMSLDIIKGR 137

[Close]

BLAST of MS020271.1 vs. TAIR 10
Match: AT3G46010.1 (actin depolymerizing factor 1 )

HSP 1 Score: 176.8 bits (447), Expect = 1.3e-44
Identity = 78/136 (57.35%), Postives = 109/136 (80.15%), Query Frame = 0


Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N  S M V++  K  F EL+ K+ +R++++K++EK+++VVVEK G P ++YE+F A LP 
Sbjct: 3   NAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLPA 62

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           ++CRYA+YDFDFVT++NCQKSKIFFIAW P  +++R+KM+YA+SKD F+ ELDGI  E+Q
Sbjct: 63  DECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQ 122

Query: 128 ATDPAEMDLEVIRDRA 144
           ATDP EMDL+V R RA
Sbjct: 123 ATDPTEMDLDVFRSRA 138

[Close]

BLAST of MS020271.1 vs. TAIR 10
Match: AT3G46010.2 (actin depolymerizing factor 1 )

HSP 1 Score: 176.8 bits (447), Expect = 1.3e-44
Identity = 78/136 (57.35%), Postives = 109/136 (80.15%), Query Frame = 0


Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N  S M V++  K  F EL+ K+ +R++++K++EK+++VVVEK G P ++YE+F A LP 
Sbjct: 14  NAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLPA 73

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           ++CRYA+YDFDFVT++NCQKSKIFFIAW P  +++R+KM+YA+SKD F+ ELDGI  E+Q
Sbjct: 74  DECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQ 133

Query: 128 ATDPAEMDLEVIRDRA 144
           ATDP EMDL+V R RA
Sbjct: 134 ATDPTEMDLDVFRSRA 149

The following BLAST results are available for this feature:

BLAST of MS020271.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145996.1	2.5e-71	98.58	actin-depolymerizing factor [Momordica charantia]	[more]
TYK20532.1	2.1e-70	96.45	actin-depolymerizing factor [Cucumis melo var. makuwa]	[more]
XP_038888227.1	2.8e-70	97.16	actin-depolymerizing factor [Benincasa hispida]	[more]
XP_008447430.1	6.2e-70	96.45	PREDICTED: actin-depolymerizing factor [Cucumis melo] >XP_008447431.1 PREDICTED:...	[more]
XP_004151983.1	1.4e-69	95.74	actin-depolymerizing factor [Cucumis sativus] >XP_011651442.1 actin-depolymerizi...	[more]

BLAST of MS020271.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8SAG3	8.7e-59	79.41	Actin-depolymerizing factor OS=Vitis vinifera OX=29760 PE=2 SV=1	[more]
Q9ZSK2	2.2e-54	72.14	Actin-depolymerizing factor 6 OS=Arabidopsis thaliana OX=3702 GN=ADF6 PE=1 SV=1	[more]
Q2QLT8	7.9e-52	69.85	Actin-depolymerizing factor 11 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF11...	[more]
Q9AY76	1.5e-50	69.85	Actin-depolymerizing factor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF2 P...	[more]
Q337A5	1.8e-48	68.22	Actin-depolymerizing factor 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF10...	[more]

BLAST of MS020271.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CY17	1.2e-71	98.58	actin-depolymerizing factor OS=Momordica charantia OX=3673 GN=LOC111015308 PE=3 ...	[more]
A0A5D3DAD6	1.0e-70	96.45	Actin-depolymerizing factor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BHG1	3.0e-70	96.45	actin-depolymerizing factor OS=Cucumis melo OX=3656 GN=LOC103489879 PE=3 SV=1	[more]
A0A6J1K588	3.0e-70	96.45	actin-depolymerizing factor-like OS=Cucurbita maxima OX=3661 GN=LOC111491296 PE=...	[more]
A0A6J1EY99	3.0e-70	96.45	actin-depolymerizing factor-like OS=Cucurbita moschata OX=3662 GN=LOC111437311 P...	[more]

BLAST of MS020271.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G31200.1	1.6e-55	72.14	actin depolymerizing factor 6	[more]
AT4G00680.1	7.9e-47	60.00	actin depolymerizing factor 8	[more]
AT1G01750.1	1.1e-45	58.52	actin depolymerizing factor 11	[more]
AT3G46010.1	1.3e-44	57.35	actin depolymerizing factor 1	[more]
AT3G46010.2	1.3e-44	57.35	actin depolymerizing factor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002108	Actin-depolymerising factor homology domain	SMART	SM00102	adf_2	coord: 17..144 e-value: 9.1E-54 score: 194.6
IPR002108	Actin-depolymerising factor homology domain	PFAM	PF00241	Cofilin_ADF	coord: 20..141 e-value: 1.2E-38 score: 131.6
IPR002108	Actin-depolymerising factor homology domain	PROSITE	PS51263	ADF_H	coord: 11..144 score: 39.587761
IPR029006	ADF-H/Gelsolin-like domain superfamily	GENE3D	3.40.20.10	Severin	coord: 9..144 e-value: 2.6E-51 score: 175.0
IPR017904	ADF/Cofilin	PANTHER	PTHR11913	COFILIN-RELATED	coord: 5..144
IPR017904	ADF/Cofilin	CDD	cd11286	ADF_cofilin_like	coord: 11..143 e-value: 2.14989E-61 score: 183.142
None	No IPR available	PANTHER	PTHR11913:SF96	COFILIN/ACTIN-DEPOLYMERIZING FACTOR-LIKE PROTEIN	coord: 5..144
None	No IPR available	SUPERFAMILY	55753	Actin depolymerizing proteins	coord: 7..142

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS020271	MS020271	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS020271.1-cds	MS020271.1-cds-scaffold665:647246..647529	CDS
MS020271.1-cds	MS020271.1-cds-scaffold665:648072..648219	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS020271.1	MS020271.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030042	actin filament depolymerization
cellular_component	GO:0015629	actin cytoskeleton
molecular_function	GO:0003779	actin binding