MS020271 (gene) Bitter gourd (TR) v1

Overview
NameMS020271
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionactin-depolymerizing factor
Locationscaffold665: 647246 .. 648219 (+)
RNA-Seq ExpressionMS020271
SyntenyMS020271
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TATGAATTACTTTTGCAGCAAAACACTTTATCTGCAATGGGTGTGAATGAACACACCAAGAAAACGTTTTCAGAACTACAGAGAAAGAAAATGTACCGATATGTGATCTTTAAGGTTGATGAAAAGAAAAGGGAGGTGGTGGTTGAAAAGACTGGCAGTCCTGCAGAGAGCTATGAAGATTTCACTGCCGCTCTTCCCGACAATGATTGCCGCTATGCCGTGTATGATTTTGATTTCGTGACATCTGACAACTGTCAGAAGAGCAAGATATTCTTCATCGCATGGTTAGCTTTCAACTATTATGAACTCAATCTTATCTTTCTTATTGCTATGTTAATTTGATTATACTATTGCTCATTGAATTGCCCCACAACACAAAACTTTGGATACTAAGGAAAACAATGGCTATCCTAAGGTCATAAGAGTTTTGATCCTACAACCACCGATTCTAACCTGTGACTATTGAAAATCAAACTACCATTTCTAAGCTTTATGGTTCTTCATGTTCATGGTCAAATGGTTATTCATGAAACTCATCTGGTATGCTTACTATTTTTATTAAATACCTACTTAGTCTTCTGTTAGAGTTTAGTGGTAAGAGTTCTATATGAATCTCCACTACTCTCCGAATATCATAGCATATCAAAATTCTTGTCCCTTGATATTTTTGGGCAAAACTGAAAAATCCTGTCATATTTTTACCAGAATGATTGCTTCTGTTGTTGGTGATCCAATGCACCTTTTGAATCTCTTATGCTGAAAACCATCAAAGTTATGACCAGGTGTTTTTTCTCTCTTGATCTCACACTAGATTGTCGTACTTCAGGTCTCCAGCTAGCTCACGAATCCGTGCTAAGATGCTCTATGCGACATCTAAAGACAACTTTCGGCACGAGCTTGATGGCATCCACTATGAGATTCAAGCTACTGACCCTGCAGAGATGGACCTGGAAGTGATTAGAGACCGTGCTCAA

mRNA sequence

TATGAATTACTTTTGCAGCAAAACACTTTATCTGCAATGGGTGTGAATGAACACACCAAGAAAACGTTTTCAGAACTACAGAGAAAGAAAATGTACCGATATGTGATCTTTAAGGTTGATGAAAAGAAAAGGGAGGTGGTGGTTGAAAAGACTGGCAGTCCTGCAGAGAGCTATGAAGATTTCACTGCCGCTCTTCCCGACAATGATTGCCGCTATGCCGTGTATGATTTTGATTTCGTGACATCTGACAACTGTCAGAAGAGCAAGATATTCTTCATCGCATGGTCTCCAGCTAGCTCACGAATCCGTGCTAAGATGCTCTATGCGACATCTAAAGACAACTTTCGGCACGAGCTTGATGGCATCCACTATGAGATTCAAGCTACTGACCCTGCAGAGATGGACCTGGAAGTGATTAGAGACCGTGCTCAA

Coding sequence (CDS)

TATGAATTACTTTTGCAGCAAAACACTTTATCTGCAATGGGTGTGAATGAACACACCAAGAAAACGTTTTCAGAACTACAGAGAAAGAAAATGTACCGATATGTGATCTTTAAGGTTGATGAAAAGAAAAGGGAGGTGGTGGTTGAAAAGACTGGCAGTCCTGCAGAGAGCTATGAAGATTTCACTGCCGCTCTTCCCGACAATGATTGCCGCTATGCCGTGTATGATTTTGATTTCGTGACATCTGACAACTGTCAGAAGAGCAAGATATTCTTCATCGCATGGTCTCCAGCTAGCTCACGAATCCGTGCTAAGATGCTCTATGCGACATCTAAAGACAACTTTCGGCACGAGCTTGATGGCATCCACTATGAGATTCAAGCTACTGACCCTGCAGAGATGGACCTGGAAGTGATTAGAGACCGTGCTCAA

Protein sequence

YELLLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPAEMDLEVIRDRAQ
Homology
BLAST of MS020271 vs. NCBI nr
Match: XP_022145996.1 (actin-depolymerizing factor [Momordica charantia])

HSP 1 Score: 278.9 bits (712), Expect = 2.5e-71
Identity = 139/141 (98.58%), Postives = 140/141 (99.29%), Query Frame = 0

Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

BLAST of MS020271 vs. NCBI nr
Match: TYK20532.1 (actin-depolymerizing factor [Cucumis melo var. makuwa])

HSP 1 Score: 275.8 bits (704), Expect = 2.1e-70
Identity = 136/141 (96.45%), Postives = 140/141 (99.29%), Query Frame = 0

Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           +L+QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESYEDFTA
Sbjct: 7   VLEQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTA 66

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 67  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 126

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 127 YEIQATDPAEMDLEVIRDRAQ 147

BLAST of MS020271 vs. NCBI nr
Match: XP_038888227.1 (actin-depolymerizing factor [Benincasa hispida])

HSP 1 Score: 275.4 bits (703), Expect = 2.8e-70
Identity = 137/141 (97.16%), Postives = 139/141 (98.58%), Query Frame = 0

Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K GSPAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGSPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

BLAST of MS020271 vs. NCBI nr
Match: XP_008447430.1 (PREDICTED: actin-depolymerizing factor [Cucumis melo] >XP_008447431.1 PREDICTED: actin-depolymerizing factor [Cucumis melo] >XP_022930980.1 actin-depolymerizing factor-like [Cucurbita moschata] >XP_022995915.1 actin-depolymerizing factor-like [Cucurbita maxima] >XP_023533852.1 actin-depolymerizing factor-like [Cucurbita pepo subsp. pepo] >KAG6606377.1 Actin-depolymerizing factor 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036318.1 Actin-depolymerizing factor 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 274.2 bits (700), Expect = 6.2e-70
Identity = 136/141 (96.45%), Postives = 139/141 (98.58%), Query Frame = 0

Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

BLAST of MS020271 vs. NCBI nr
Match: XP_004151983.1 (actin-depolymerizing factor [Cucumis sativus] >XP_011651442.1 actin-depolymerizing factor [Cucumis sativus] >KGN57913.1 hypothetical protein Csa_010146 [Cucumis sativus])

HSP 1 Score: 273.1 bits (697), Expect = 1.4e-69
Identity = 135/141 (95.74%), Postives = 139/141 (98.58%), Query Frame = 0

Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIF+VDEKKREVVV+K G+PAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

BLAST of MS020271 vs. ExPASy Swiss-Prot
Match: Q8SAG3 (Actin-depolymerizing factor OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 8.7e-59
Identity = 108/136 (79.41%), Postives = 124/136 (91.18%), Query Frame = 0

Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N  S MGV +H+K TF EL+RKK++RYVIFK+DEKK+EVVVEKTG PAES+++F AALP+
Sbjct: 7   NASSGMGVADHSKNTFLELKRKKVHRYVIFKIDEKKKEVVVEKTGGPAESFDEFAAALPE 66

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           NDCRYAVYDFDFVTS+NCQKSKIFFIAWSP SSRIRAKMLYATSK+ FR ELDG+HYEIQ
Sbjct: 67  NDCRYAVYDFDFVTSENCQKSKIFFIAWSPDSSRIRAKMLYATSKERFRRELDGVHYEIQ 126

Query: 128 ATDPAEMDLEVIRDRA 144
           ATDP EMDLEV+R+RA
Sbjct: 127 ATDPTEMDLEVLRERA 142

BLAST of MS020271 vs. ExPASy Swiss-Prot
Match: Q9ZSK2 (Actin-depolymerizing factor 6 OS=Arabidopsis thaliana OX=3702 GN=ADF6 PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 2.2e-54
Identity = 101/140 (72.14%), Postives = 120/140 (85.71%), Query Frame = 0

Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L + N +S MGV + +K TF ELQRKK +RYV+FK+DE K+EVVVEKTG+P ESY+DF A
Sbjct: 6   LSRPNAISGMGVADESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           +LPDNDCRYAVYDFDFVTS+NCQKSKIFF AWSP++S IRAK+LY+TSKD    EL GIH
Sbjct: 66  SLPDNDCRYAVYDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRELQGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRA 144
           YEIQATDP E+DLEV+R+RA
Sbjct: 126 YEIQATDPTEVDLEVLRERA 145

BLAST of MS020271 vs. ExPASy Swiss-Prot
Match: Q2QLT8 (Actin-depolymerizing factor 11 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF11 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 7.9e-52
Identity = 95/136 (69.85%), Postives = 118/136 (86.76%), Query Frame = 0

Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N  S +GV    K+TF ELQRKK +RYVIFK+D+K +EVVVEKTGS  ES++DF  +LP+
Sbjct: 9   NASSGIGVAAECKQTFLELQRKKSHRYVIFKIDDKCKEVVVEKTGSSTESFDDFMDSLPE 68

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           +DCRYA+YDFDFVT +NCQKSKIFF+AWSP+ SRIRAKMLYATSK+ FR ELDG+HYEIQ
Sbjct: 69  SDCRYAIYDFDFVTEENCQKSKIFFVAWSPSVSRIRAKMLYATSKERFRRELDGVHYEIQ 128

Query: 128 ATDPAEMDLEVIRDRA 144
           ATDP+E+D+E++R+RA
Sbjct: 129 ATDPSELDIELLRERA 144

BLAST of MS020271 vs. ExPASy Swiss-Prot
Match: Q9AY76 (Actin-depolymerizing factor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF2 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 1.5e-50
Identity = 95/136 (69.85%), Postives = 115/136 (84.56%), Query Frame = 0

Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N  S MGV    + TF ELQ KK +RYVIFK++EK+++VVVEKTG+  ESY+DF A+LP+
Sbjct: 9   NASSGMGVAPDIRDTFLELQMKKAFRYVIFKIEEKQKQVVVEKTGATTESYDDFLASLPE 68

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           NDCRYA+YDFDFVT +N QKSKIFFIAWSP++SRIRAKMLY+TSKD  + ELDG HYEIQ
Sbjct: 69  NDCRYALYDFDFVTGENVQKSKIFFIAWSPSTSRIRAKMLYSTSKDRIKQELDGFHYEIQ 128

Query: 128 ATDPAEMDLEVIRDRA 144
           ATDP E+DLEV+R+RA
Sbjct: 129 ATDPTEVDLEVLRERA 144

BLAST of MS020271 vs. ExPASy Swiss-Prot
Match: Q337A5 (Actin-depolymerizing factor 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF10 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 1.8e-48
Identity = 88/129 (68.22%), Postives = 113/129 (87.60%), Query Frame = 0

Query: 15  VNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPDNDCRYAV 74
           V E +K  F EL+R+K++RYVIFK+D+++ E+VVEKTG+P ESY+DFTA+LP +DCRYAV
Sbjct: 20  VPEKSKSAFWELKRRKVHRYVIFKIDDRREEIVVEKTGAPGESYDDFTASLPADDCRYAV 79

Query: 75  YDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPAEM 134
           YD DFV+ DNC+KSKIFFI+WSP+ SRIRAK +YA S++ FRHELDG+H+EIQATDP +M
Sbjct: 80  YDLDFVSDDNCRKSKIFFISWSPSVSRIRAKTIYAVSRNQFRHELDGVHFEIQATDPDDM 139

Query: 135 DLEVIRDRA 144
           DLEV+R RA
Sbjct: 140 DLEVLRGRA 148

BLAST of MS020271 vs. ExPASy TrEMBL
Match: A0A6J1CY17 (actin-depolymerizing factor OS=Momordica charantia OX=3673 GN=LOC111015308 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.2e-71
Identity = 139/141 (98.58%), Postives = 140/141 (99.29%), Query Frame = 0

Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

BLAST of MS020271 vs. ExPASy TrEMBL
Match: A0A5D3DAD6 (Actin-depolymerizing factor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G001160 PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.0e-70
Identity = 136/141 (96.45%), Postives = 140/141 (99.29%), Query Frame = 0

Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           +L+QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESYEDFTA
Sbjct: 7   VLEQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTA 66

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 67  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 126

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 127 YEIQATDPAEMDLEVIRDRAQ 147

BLAST of MS020271 vs. ExPASy TrEMBL
Match: A0A1S3BHG1 (actin-depolymerizing factor OS=Cucumis melo OX=3656 GN=LOC103489879 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.0e-70
Identity = 136/141 (96.45%), Postives = 139/141 (98.58%), Query Frame = 0

Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

BLAST of MS020271 vs. ExPASy TrEMBL
Match: A0A6J1K588 (actin-depolymerizing factor-like OS=Cucurbita maxima OX=3661 GN=LOC111491296 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.0e-70
Identity = 136/141 (96.45%), Postives = 139/141 (98.58%), Query Frame = 0

Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

BLAST of MS020271 vs. ExPASy TrEMBL
Match: A0A6J1EY99 (actin-depolymerizing factor-like OS=Cucurbita moschata OX=3662 GN=LOC111437311 PE=3 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.0e-70
Identity = 136/141 (96.45%), Postives = 139/141 (98.58%), Query Frame = 0

Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L +QNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVV+K G+PAESYEDFTA
Sbjct: 6   LRRQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH
Sbjct: 66  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRAQ 145
           YEIQATDPAEMDLEVIRDRAQ
Sbjct: 126 YEIQATDPAEMDLEVIRDRAQ 146

BLAST of MS020271 vs. TAIR 10
Match: AT2G31200.1 (actin depolymerizing factor 6 )

HSP 1 Score: 213.0 bits (541), Expect = 1.6e-55
Identity = 101/140 (72.14%), Postives = 120/140 (85.71%), Query Frame = 0

Query: 4   LLQQNTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTA 63
           L + N +S MGV + +K TF ELQRKK +RYV+FK+DE K+EVVVEKTG+P ESY+DF A
Sbjct: 6   LSRPNAISGMGVADESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLA 65

Query: 64  ALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIH 123
           +LPDNDCRYAVYDFDFVTS+NCQKSKIFF AWSP++S IRAK+LY+TSKD    EL GIH
Sbjct: 66  SLPDNDCRYAVYDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRELQGIH 125

Query: 124 YEIQATDPAEMDLEVIRDRA 144
           YEIQATDP E+DLEV+R+RA
Sbjct: 126 YEIQATDPTEVDLEVLRERA 145

BLAST of MS020271 vs. TAIR 10
Match: AT4G00680.1 (actin depolymerizing factor 8 )

HSP 1 Score: 184.1 bits (466), Expect = 7.9e-47
Identity = 81/135 (60.00%), Postives = 112/135 (82.96%), Query Frame = 0

Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N+ S M VN+  K  F EL+ K+ YR+++FK+DEK ++V +EK G+P E+Y+DFT+++PD
Sbjct: 3   NSASGMHVNDECKIKFLELKAKRTYRFIVFKIDEKAQQVQIEKLGNPEETYDDFTSSIPD 62

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           ++CRYAVYDFDF T DNCQKSKIFFIAWSP +SR+R+KMLYA+SKD F+ E++GI  E+Q
Sbjct: 63  DECRYAVYDFDFTTEDNCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKREMEGIQVELQ 122

Query: 128 ATDPAEMDLEVIRDR 143
           ATDP+EM L++I+ R
Sbjct: 123 ATDPSEMSLDIIKGR 137

BLAST of MS020271 vs. TAIR 10
Match: AT1G01750.1 (actin depolymerizing factor 11 )

HSP 1 Score: 180.3 bits (456), Expect = 1.1e-45
Identity = 79/135 (58.52%), Postives = 112/135 (82.96%), Query Frame = 0

Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N+ S M V++  K  F EL+ K+ YR+++FK+DEK ++V+++K G+P E+YEDFT ++P+
Sbjct: 3   NSASGMHVSDECKLKFLELKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSIPE 62

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           ++CRYAVYD+DF T +NCQKSKIFFIAWSP +SR+R+KMLYA+SKD F+ ELDGI  E+Q
Sbjct: 63  DECRYAVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVELQ 122

Query: 128 ATDPAEMDLEVIRDR 143
           ATDP+EM L++I+ R
Sbjct: 123 ATDPSEMSLDIIKGR 137

BLAST of MS020271 vs. TAIR 10
Match: AT3G46010.1 (actin depolymerizing factor 1 )

HSP 1 Score: 176.8 bits (447), Expect = 1.3e-44
Identity = 78/136 (57.35%), Postives = 109/136 (80.15%), Query Frame = 0

Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N  S M V++  K  F EL+ K+ +R++++K++EK+++VVVEK G P ++YE+F A LP 
Sbjct: 3   NAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLPA 62

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           ++CRYA+YDFDFVT++NCQKSKIFFIAW P  +++R+KM+YA+SKD F+ ELDGI  E+Q
Sbjct: 63  DECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQ 122

Query: 128 ATDPAEMDLEVIRDRA 144
           ATDP EMDL+V R RA
Sbjct: 123 ATDPTEMDLDVFRSRA 138

BLAST of MS020271 vs. TAIR 10
Match: AT3G46010.2 (actin depolymerizing factor 1 )

HSP 1 Score: 176.8 bits (447), Expect = 1.3e-44
Identity = 78/136 (57.35%), Postives = 109/136 (80.15%), Query Frame = 0

Query: 8   NTLSAMGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVEKTGSPAESYEDFTAALPD 67
           N  S M V++  K  F EL+ K+ +R++++K++EK+++VVVEK G P ++YE+F A LP 
Sbjct: 14  NAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLPA 73

Query: 68  NDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQ 127
           ++CRYA+YDFDFVT++NCQKSKIFFIAW P  +++R+KM+YA+SKD F+ ELDGI  E+Q
Sbjct: 74  DECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQ 133

Query: 128 ATDPAEMDLEVIRDRA 144
           ATDP EMDL+V R RA
Sbjct: 134 ATDPTEMDLDVFRSRA 149

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145996.12.5e-7198.58actin-depolymerizing factor [Momordica charantia][more]
TYK20532.12.1e-7096.45actin-depolymerizing factor [Cucumis melo var. makuwa][more]
XP_038888227.12.8e-7097.16actin-depolymerizing factor [Benincasa hispida][more]
XP_008447430.16.2e-7096.45PREDICTED: actin-depolymerizing factor [Cucumis melo] >XP_008447431.1 PREDICTED:... [more]
XP_004151983.11.4e-6995.74actin-depolymerizing factor [Cucumis sativus] >XP_011651442.1 actin-depolymerizi... [more]
Match NameE-valueIdentityDescription
Q8SAG38.7e-5979.41Actin-depolymerizing factor OS=Vitis vinifera OX=29760 PE=2 SV=1[more]
Q9ZSK22.2e-5472.14Actin-depolymerizing factor 6 OS=Arabidopsis thaliana OX=3702 GN=ADF6 PE=1 SV=1[more]
Q2QLT87.9e-5269.85Actin-depolymerizing factor 11 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF11... [more]
Q9AY761.5e-5069.85Actin-depolymerizing factor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF2 P... [more]
Q337A51.8e-4868.22Actin-depolymerizing factor 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF10... [more]
Match NameE-valueIdentityDescription
A0A6J1CY171.2e-7198.58actin-depolymerizing factor OS=Momordica charantia OX=3673 GN=LOC111015308 PE=3 ... [more]
A0A5D3DAD61.0e-7096.45Actin-depolymerizing factor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3BHG13.0e-7096.45actin-depolymerizing factor OS=Cucumis melo OX=3656 GN=LOC103489879 PE=3 SV=1[more]
A0A6J1K5883.0e-7096.45actin-depolymerizing factor-like OS=Cucurbita maxima OX=3661 GN=LOC111491296 PE=... [more]
A0A6J1EY993.0e-7096.45actin-depolymerizing factor-like OS=Cucurbita moschata OX=3662 GN=LOC111437311 P... [more]
Match NameE-valueIdentityDescription
AT2G31200.11.6e-5572.14actin depolymerizing factor 6 [more]
AT4G00680.17.9e-4760.00actin depolymerizing factor 8 [more]
AT1G01750.11.1e-4558.52actin depolymerizing factor 11 [more]
AT3G46010.11.3e-4457.35actin depolymerizing factor 1 [more]
AT3G46010.21.3e-4457.35actin depolymerizing factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002108Actin-depolymerising factor homology domainSMARTSM00102adf_2coord: 17..144
e-value: 9.1E-54
score: 194.6
IPR002108Actin-depolymerising factor homology domainPFAMPF00241Cofilin_ADFcoord: 20..141
e-value: 1.2E-38
score: 131.6
IPR002108Actin-depolymerising factor homology domainPROSITEPS51263ADF_Hcoord: 11..144
score: 39.587761
IPR029006ADF-H/Gelsolin-like domain superfamilyGENE3D3.40.20.10Severincoord: 9..144
e-value: 2.6E-51
score: 175.0
IPR017904ADF/CofilinPANTHERPTHR11913COFILIN-RELATEDcoord: 5..144
IPR017904ADF/CofilinCDDcd11286ADF_cofilin_likecoord: 11..143
e-value: 2.14989E-61
score: 183.142
NoneNo IPR availablePANTHERPTHR11913:SF96COFILIN/ACTIN-DEPOLYMERIZING FACTOR-LIKE PROTEINcoord: 5..144
NoneNo IPR availableSUPERFAMILY55753Actin depolymerizing proteinscoord: 7..142

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS020271.1MS020271.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030042 actin filament depolymerization
cellular_component GO:0015629 actin cytoskeleton
molecular_function GO:0003779 actin binding