MS016288.1 (mRNA) Bitter gourd (TR) v1

Overview
NameMS016288.1
TypemRNA
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAmino acid transporter, putative
Locationscaffold1407: 103550 .. 109630 (-)
Sequence length1356
RNA-Seq ExpressionMS016288.1
SyntenyMS016288.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGAGCTTCCTCCGGCGGACTCATTTCCCGCATCCCGAAGCAAAAACGACGCCGGAGCAGCCTTCGTCCTCGAATCCAAAGGTCCTCCGAATTCTCTCGATTATTTTGGTAATCCAATTACTAATTGATGAAATTGAATTGAACGTGAACAAATTACAAATTACAAAACAGGGGAGTGGTGGCACGCCGGATTCCACTTGACGACGGCGATCGTAGGGCCGCCGATTCTGACGCTTCCCTTCGCCTTCCGAGGATTGGGTTGGATACTAGGATTTCTGTGTTTGACGGCCATGGCCGCCGTCACCTTCTACTCCTACTACCTCTTGTCCAAAGTTCTCGAGCACTGTGAAAAACACGGCCGCCGCCACATCCGCTTCCGGGAACTCGCCGCCGATCTCTTAGGTAACCTTTTTTTCTTTCTTTTCCCCACTACACCCCCGTCCCTTTCTTACAATATATATATTTTTTTAGTTTAAATCCTCGGAACTTAAAAAAAAATACATACAGTACTTGATCATATTAATTCATTATTAATATACATATTCCAAGTTAAGTCGTTAATATGTTATAAGTAAATAAAGATAATTAAGGACTGAAATTATTTTCTTTCACTAAAATTTGATTTAAACATTATTTCTTTATAAAAAGTAATTGTTTTAACAATAGAAAATAGAAATGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTCTCTCTCTCTCTCTCCAAGTAATCTATATTTTCATTGGGTAGCTATTCATGGATGGTTATTAAAAATAGTGCATATAAATAAAGTAGAGTAAAGGAGATGGAAATACTAAGAATTGTTTCTAAACTATGTATATCTAAACATGTTTTCTGAGCTAATTTTTATTATATAATACTGCATATATTTTACTTTTTTTTCCCTCTAAAAAGTGACATTGTTCGAGAGACCTACAAATTCCCACCTAGTTGGTCGTCTTTTGAAAAGACGATCATATATTAATTTTTTTAAAAGAAATTTTACGGTGGCGCTTTAGGTAGTCTATCTATTTTCTAAGCATTATTCTTGGAAATAGTTTGGATAGTGTCATATATTTGGAATAAAATTTTAAAATGGCATTATTAAAAAAAAATCTCATTTTTATCCCACTAAGACTTTTCTAAGTGGTTAAATGCATCAAAATGATGATACTCGTCTAAGTCTTTTGCTATTTGATCTTTAATCTTTCAAAATTATTTGATCTTTAATCTTTTAAAATTCTCAACTTAACCATTTCATTAAAAAAGTATATCGAAATTTGTTAGTAAAAAACTCATGTATTATTAAATGGTTATTTTGTTTACATATTTGGACACGTGAGAGCACACCATTTAGACATGTCATTTAGAAGAAATTAATTTTATTGATGGGTAGATGAATTTCATGTGTCAAATAGCACCTAATTGAAATGTCTAACATGTCCAAATTCGTTTATATAAAAAGTAGTTTAATATTATATTTATAAAAATTTTAAAAATTATTTAAAAATACATAGGTACAAAAAACACGTATTTTAAATTATTTTTTAAAATACATATGTACAAAAATTTTAGTAGAGTAAATTCAATTTTTTTTCAATAAACCATTTTTTCTGACCAATACAGACGCAATTATTAGTCAAAGACGAGTCTAAAATTCCATTTAAAAAATAAAATAAAATATAACCAATTGATTGGTCCAATTAGAGAGGGAATGCTCCAAATAATATTTAACTCCCTCAATTCACAATAATGAATTTTCCTTCACTAATAATTGCTGAATTCCACATTATTCTTAAAAATTTTCAACAAGTTAATAACGAATGAAAAAGTATATTACCGACATGAGCTTATATCATCGACTGATTCTGATTAAAAAGTTAGAAATTTAAATTTTTATTTCTATACGTTTTTTATCTAAAAGAAAGAAAGTAAATATCTATATAACGATACAACATTGAAACTTTTCAAGATAGGAAAGTGAATTTCAAGAATAGTAATTAAAGAAGAGAAGGGGAGACAGAGAACACGAAGGAATAGACGAAAGAGAGACGTGAGGGAGAGGAAGAGAATGTGGTAATTAATGGGAAAATGAAACATAAAAGTGAAAATTCATTTGTTTTGTTTTTCTTTTTAGTACAAATATGAGGTGGTAATGCAAATTCACGACCTTTTAATCAGATATAAGTACTTAAATCGATTGAGCTAGCTCAAGTTGGCGTGAAAAGTTCATTTTTTATTCTCTCTTGGTGATGTGAGTCATAAATTAATACTATAAATATTAAATTTAATCAAATTTGATCTTAAATTTAAAGGGTTGAAATTGTTATCTTCAGTTTAGCCTCCATTCGAAATATTATTAAAAAAATATATTCTCATATTGGTTCAAATAAATGTATGATAAAAGAAAATCGATGAGACTTAAAGCAAATAAAAGACCAACACTGCAACTATATTTTTGAAATTTATGGTTTTCATTCAATGGTAGAAATTTTGCATGGATTAATTCCTTGGACTAAATATTTAAGGCTCATTTTATGTTTATCCTTTTTAGCTTCTTTTTTTTCATAAAAAAATAAAATTTGTGTAGCAATTTAAAATTTTTTTAAAGAAACATAAAATATAAAACAAAAGAAAATAAATTGTTATTATAAATCTTATCATTGAAAAATGTAAAATAAGATAATTACTAAATCAAATCGGATCATTAAAACATTATCGCAATGCCATCATGCGCCCATAGACATGTAAGTCCGCACTCNNNNNNNNNNNNNNNNNNNNNNNNNAAAAATTAATTAATCATTGATTAATTTTTTTTAGTCCATTAAATTCTTATTATTGATTAACTTTAATGATGGTAAATTCATATTTTTTTATTTTATAATATATAAATTATAGTAATTTTTTTATTTAAATACGGGGATGAGAAATTTTTTCTTTTTTTTAAACAAGGAGACCACGATGTACTTAAAAAATAAAATTAAAAAAAAAAGACTAGGAATTAAACCTTCAAAGATATCTTAGTCTTGTTTAATAAATATTGACAAAAAATTATAGATTATATATAATTGTCAATTAATATGTGTGTATTTTAATATTTGTTATATTTTATTTAAATTATGATTTCAACTGCAGTTAAATGATCAATACGAGCAATGTGTTTAGATTAATATGTGTTATCTTTCGGTGGAAAATATATATTTTAACCGATTGAATTATGTTAGATTGATACGCATTGATTTTGAGGATGGTATTTTGGTAATTTGACAGGGTCAGGATGGATGCTATATTTTGTGGTATTCATACAAGCGGCTGTGAACACAGGAGTGGGAGTAGCCGCAATTTTGCTTGGTGGGGAGTGCATGGAGGTATTCTTTCTATTATTCTATAATGACACAACTTTTCTCATTCAAAAACCATTTAATTATAGATTGGATTATCCATTTGTTTCTTAATTTTAATTCAGTCCTTATAATTTTAAGATTTTTAATTTTGGTCCTACATAGCTTACGAGAGATTAATTGAAACTAAATCCAAATCAAAGAGATAGATTTTTTACTGTTTGAGAATGTTACATCCACCACCATTCAGAGAAAGTCTAGAAGTTTCAATAATTAATTAATTCTAATTCTAATTGGGTCAAATCACAACCTCACAAGTCTTTAAATCATCTTTTATCCATCTCTTTTTATCTTATATATATTTTTAAAAAAGTATTGTGATATTTTAATTCAAGGCTGTAAATTAATATGAGCATGGTTAGTTGGTTTACAAGTTTGAAATTTGGTTAGTGATGAGTTTTGGTGATTTGTTGATGGAAAAAAAAGTTGATGTATTGGAGGTTATATCCAAAAGGGGAGCTGAAGCTGTACCATTTCATAGCAATGGTGAGTGTGGGAATGATAATGATCTCTCAAATTCCCACTTTCCACTCCCTCAGACACATCAATTTTGCTTCTCTTCTCCTCAGTCTGGCCTACACTTTCTTCATCGCCTCTGCTTCCATTCTTGCAGGTTAGTGTGTGTATATATATCTATTTAATAACTAACCGTTTCCATTTCATCTGGTTAATTCGTATTTGTTTCATTTTGGTCTCTCCAACTTTCGTAACTTTTATCATTCAACTATTTTGATCTTTATATTTTTATTCGTTTTAGTACACGTGCTTTCAAAGCACCGGTAAAAAATGACCATTTTTTACTCCCTATTTTAAATTTTTAACACGAATTCTATACACAATGAAACCTTTTAATACGAAGCTATGAATATATATATATTTCAAGGAATTCATTGAATATTTGTGGGTAGAAATTGTGATTTAAAATGTGAGATAAAAAAGAAAATAAAATAACTAAAATAGAACGCTTTGAAAGTATGTATGAATATTAAAATGGATAAAAGTCAGAAAGTATATGTATATAGAGACCATATATAGTAATTAAACCATTTGAAAGTAGAAATATTTAAACTGATCGTCAAATTGAAAGTTCATAGACCAAAATAAACTTTTAATAGTTTTTTAACTAGGAACCTTTTAAGTAAGTTTTATACTTATCTTAGGACTCCCTTATTAACGGGGCATGACACCATATGATAATGTATTTAATAAGCTTAATATAAAAAATTCTTAATCATTTTCAAGGGTGGACTTTGGCGGGCACGGGAAGCTAAGAGTTATACAGATTCAGATTAAAAGATAAATAAATAAATAAGTTTTGTTGGTTCTCTTGAAGCATAGATTTGAATGGAATTTCAGGTACATCAGATAAGGTTCCTCCGAGGGACTATCATTTGGAATCGACTCTATCTGAAAGAGTTTTTAGCGCCTTCACTTCCATTTCCATCTTCGCTGCCATTTTTGGCAATGGCATTCTTCCTGAAATTCAAGTAATATTTTACCACTTTTCATTTCTAATATCCCATTTTCATTACCATTATTTGTTTATTTAGCTTTTATAATTACCAATATGATACTTCCTATATTTAGAGTTATGGTAGGGTCAAAACTTCATAAATCATTTACCAGCAGTATTCGTATGTAATTGAAATAGTGGCACATTTTAGACCCACTGACTGGTCTAGGATTGAGTAAAAAATAAAGATTTTCAGGTAGAAAAAACGATTTATGCAACGACTAAACCATAGAGTAGATCAAAATTGAAACGTTTAGAACTCCAACGAGTGATTTGAAACTAAATAAAGTTGAAATAATAGAAATTAGATTGAAAACTTTAAAAAACTAAGAAGAAAGTGTGTTTTTGAACTATCAAATAGGGTCCTTGTGAATTAGGTACACAAGTTCAAAGTTTTTTATTTTACATGAAGCTCTTATATATACTTAAATGTATATGCAGGCAACTCTTGCACCACCAGTGGCTGGGAAGATGGTAAAAGGGCTCATCATGTGTTACATTGTAATCTTCATCACATTCTACTCATCTGCAGCTTCTGGCTACTGGGTTTTTGGCAACAGATCCAATTCAAATCTCTTAAAGAACTTACTCCCCAAAAATGAATCTCCTTTGGCTCCCACTTGGCTTCTCTTCCTCGCTGTTCTCTTCATTCTCCTCCAACTTCTCGCCATCGGAATGGTCCGAAAAACGTCCATTCTCTCAAAACCTCATTTGATCACGATTTCATTTTTAGTCTTCTGATCTCGCCTGTGCAGGTCTACGCCCAAGTGGCTTACGAGATAATTGAGAGAAGGTCAGGAGATGCACAACAAGGAGTGTTCTCAAGGAGAAACCTGATTCCTAGGCTGATTTTGAGGACATTGTACATGGCTATGTGTGGTTTTTTGGCAGCCATGCTTCCATTCTTCGGTGACATTAACAGCGTTGTTGGAGCGATCGGGTTCATCCCGCTCGATTTCGTTCTTCCGATGGTTCTCTACAACATCACCCACAAGCCTCCGGTGTCGTGGTCGGTGACTTATTGGGTCAATGTCTTCATTATTGTTGCATTCTCTGGTGCAGGAATTATGGGATGCTTTGCTTCTGTTAGGAACTTGGTTTTGGATGCCAAGAAGTTTAAGTTGTTTAGTAGTGATGTGGTT

mRNA sequence

ATGGCAGAGCTTCCTCCGGCGGACTCATTTCCCGCATCCCGAAGCAAAAACGACGCCGGAGCAGCCTTCGTCCTCGAATCCAAAGGGGAGTGGTGGCACGCCGGATTCCACTTGACGACGGCGATCGTAGGGCCGCCGATTCTGACGCTTCCCTTCGCCTTCCGAGGATTGGGTTGGATACTAGGATTTCTGTGTTTGACGGCCATGGCCGCCGTCACCTTCTACTCCTACTACCTCTTGTCCAAAGTTCTCGAGCACTGTGAAAAACACGGCCGCCGCCACATCCGCTTCCGGGAACTCGCCGCCGATCTCTTAGGGTCAGGATGGATGCTATATTTTGTGGTATTCATACAAGCGGCTGTGAACACAGGAGTGGGAGTAGCCGCAATTTTGCTTGGTGGGGAGTGCATGGAGTTGATGTATTGGAGGTTATATCCAAAAGGGGAGCTGAAGCTGTACCATTTCATAGCAATGGTGAGTGTGGGAATGATAATGATCTCTCAAATTCCCACTTTCCACTCCCTCAGACACATCAATTTTGCTTCTCTTCTCCTCAGTCTGGCCTACACTTTCTTCATCGCCTCTGCTTCCATTCTTGCAGGTACATCAGATAAGGTTCCTCCGAGGGACTATCATTTGGAATCGACTCTATCTGAAAGAGTTTTTAGCGCCTTCACTTCCATTTCCATCTTCGCTGCCATTTTTGGCAATGGCATTCTTCCTGAAATTCAAGCAACTCTTGCACCACCAGTGGCTGGGAAGATGGTAAAAGGGCTCATCATGTGTTACATTGTAATCTTCATCACATTCTACTCATCTGCAGCTTCTGGCTACTGGGTTTTTGGCAACAGATCCAATTCAAATCTCTTAAAGAACTTACTCCCCAAAAATGAATCTCCTTTGGCTCCCACTTGGCTTCTCTTCCTCGCTGTTCTCTTCATTCTCCTCCAACTTCTCGCCATCGGAATGGTCTACGCCCAAGTGGCTTACGAGATAATTGAGAGAAGGTCAGGAGATGCACAACAAGGAGTGTTCTCAAGGAGAAACCTGATTCCTAGGCTGATTTTGAGGACATTGTACATGGCTATGTGTGGTTTTTTGGCAGCCATGCTTCCATTCTTCGGTGACATTAACAGCGTTGTTGGAGCGATCGGGTTCATCCCGCTCGATTTCGTTCTTCCGATGGTTCTCTACAACATCACCCACAAGCCTCCGGTGTCGTGGTCGGTGACTTATTGGGTCAATGTCTTCATTATTGTTGCATTCTCTGGTGCAGGAATTATGGGATGCTTTGCTTCTGTTAGGAACTTGGTTTTGGATGCCAAGAAGTTTAAGTTGTTTAGTAGTGATGTGGTT

Coding sequence (CDS)

ATGGCAGAGCTTCCTCCGGCGGACTCATTTCCCGCATCCCGAAGCAAAAACGACGCCGGAGCAGCCTTCGTCCTCGAATCCAAAGGGGAGTGGTGGCACGCCGGATTCCACTTGACGACGGCGATCGTAGGGCCGCCGATTCTGACGCTTCCCTTCGCCTTCCGAGGATTGGGTTGGATACTAGGATTTCTGTGTTTGACGGCCATGGCCGCCGTCACCTTCTACTCCTACTACCTCTTGTCCAAAGTTCTCGAGCACTGTGAAAAACACGGCCGCCGCCACATCCGCTTCCGGGAACTCGCCGCCGATCTCTTAGGGTCAGGATGGATGCTATATTTTGTGGTATTCATACAAGCGGCTGTGAACACAGGAGTGGGAGTAGCCGCAATTTTGCTTGGTGGGGAGTGCATGGAGTTGATGTATTGGAGGTTATATCCAAAAGGGGAGCTGAAGCTGTACCATTTCATAGCAATGGTGAGTGTGGGAATGATAATGATCTCTCAAATTCCCACTTTCCACTCCCTCAGACACATCAATTTTGCTTCTCTTCTCCTCAGTCTGGCCTACACTTTCTTCATCGCCTCTGCTTCCATTCTTGCAGGTACATCAGATAAGGTTCCTCCGAGGGACTATCATTTGGAATCGACTCTATCTGAAAGAGTTTTTAGCGCCTTCACTTCCATTTCCATCTTCGCTGCCATTTTTGGCAATGGCATTCTTCCTGAAATTCAAGCAACTCTTGCACCACCAGTGGCTGGGAAGATGGTAAAAGGGCTCATCATGTGTTACATTGTAATCTTCATCACATTCTACTCATCTGCAGCTTCTGGCTACTGGGTTTTTGGCAACAGATCCAATTCAAATCTCTTAAAGAACTTACTCCCCAAAAATGAATCTCCTTTGGCTCCCACTTGGCTTCTCTTCCTCGCTGTTCTCTTCATTCTCCTCCAACTTCTCGCCATCGGAATGGTCTACGCCCAAGTGGCTTACGAGATAATTGAGAGAAGGTCAGGAGATGCACAACAAGGAGTGTTCTCAAGGAGAAACCTGATTCCTAGGCTGATTTTGAGGACATTGTACATGGCTATGTGTGGTTTTTTGGCAGCCATGCTTCCATTCTTCGGTGACATTAACAGCGTTGTTGGAGCGATCGGGTTCATCCCGCTCGATTTCGTTCTTCCGATGGTTCTCTACAACATCACCCACAAGCCTCCGGTGTCGTGGTCGGTGACTTATTGGGTCAATGTCTTCATTATTGTTGCATTCTCTGGTGCAGGAATTATGGGATGCTTTGCTTCTGTTAGGAACTTGGTTTTGGATGCCAAGAAGTTTAAGTTGTTTAGTAGTGATGTGGTT

Protein sequence

MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWILGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAAVNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINFASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESPLAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLYMAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIVAFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV
Homology
BLAST of MS016288.1 vs. NCBI nr
Match: XP_022145892.1 (probable GABA transporter 2 [Momordica charantia])

HSP 1 Score: 875.5 bits (2261), Expect = 1.9e-250
Identity = 449/452 (99.34%), Postives = 449/452 (99.34%), Query Frame = 0

Query: 1   MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60
           MAELPPADSFPASR KNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI
Sbjct: 1   MAELPPADSFPASRRKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60

Query: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120
           LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA
Sbjct: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120

Query: 121 VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF 180
           VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF
Sbjct: 121 VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF 180

Query: 181 ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL 240
           ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL
Sbjct: 181 ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL 240

Query: 241 PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP 300
           PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP
Sbjct: 241 PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP 300

Query: 301 LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY 360
           LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY
Sbjct: 301 LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY 360

Query: 361 MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIV 420
           MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVS  VTYWVNVFIIV
Sbjct: 361 MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSSWVTYWVNVFIIV 420

Query: 421 AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 453
           AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV
Sbjct: 421 AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 452

BLAST of MS016288.1 vs. NCBI nr
Match: XP_008437380.1 (PREDICTED: probable GABA transporter 2 [Cucumis melo])

HSP 1 Score: 771.5 bits (1991), Expect = 3.9e-219
Identity = 394/452 (87.17%), Postives = 421/452 (93.14%), Query Frame = 0

Query: 1   MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60
           M +L P  SF  S+   DAGAAFVLESKG+WWHAGFHLTTAIVGPPILTLPFAFRGLGW 
Sbjct: 1   MTDLSPPASF--SKLSTDAGAAFVLESKGQWWHAGFHLTTAIVGPPILTLPFAFRGLGWG 60

Query: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120
           +GFLCLT MAAVTFYSYYLLSKVLE CEK GRRHIRFRELAAD+LGSGWMLYFVVFIQAA
Sbjct: 61  VGFLCLTVMAAVTFYSYYLLSKVLELCEKQGRRHIRFRELAADVLGSGWMLYFVVFIQAA 120

Query: 121 VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF 180
           VNTGVGVAAILLGGEC+ELMY  +YPKGELKLYHFIA+V+VGMIMISQ+P+FHSLR+INF
Sbjct: 121 VNTGVGVAAILLGGECLELMYSNIYPKGELKLYHFIAVVTVGMIMISQLPSFHSLRYINF 180

Query: 181 ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL 240
            SLLLSLAY FFIA ASILAGTSD VPPRDY LEST S RVFSAFTSISIFAAIFGNGIL
Sbjct: 181 LSLLLSLAYAFFIAFASILAGTSDNVPPRDYSLESTPSARVFSAFTSISIFAAIFGNGIL 240

Query: 241 PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP 300
           PEIQATLAPP  GKMVKGLIMCYIVIFITFYSSAASGYWVFGN+SNSN+LKNLLPKNESP
Sbjct: 241 PEIQATLAPPTGGKMVKGLIMCYIVIFITFYSSAASGYWVFGNKSNSNILKNLLPKNESP 300

Query: 301 LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY 360
           LAPTW+L LAVLFILLQLLAIGMVYAQVAYEI+ERRS DA+QGVFSRRNLIPRLILRTLY
Sbjct: 301 LAPTWILALAVLFILLQLLAIGMVYAQVAYEIMERRSADAKQGVFSRRNLIPRLILRTLY 360

Query: 361 MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIV 420
           M++CGF AAM PFFGDINSVVGAIGFIPLDF+LPMVLYNITHKPPV+ S+TYWVNVFI+ 
Sbjct: 361 MSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPPVT-SITYWVNVFIVA 420

Query: 421 AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 453
           AFSGAG++GCFAS+RNLVLD+KKFKLFSS VV
Sbjct: 421 AFSGAGLLGCFASIRNLVLDSKKFKLFSSHVV 449

BLAST of MS016288.1 vs. NCBI nr
Match: XP_004143875.1 (probable GABA transporter 2 [Cucumis sativus] >KGN50030.1 hypothetical protein Csa_000172 [Cucumis sativus])

HSP 1 Score: 769.6 bits (1986), Expect = 1.5e-218
Identity = 391/452 (86.50%), Postives = 422/452 (93.36%), Query Frame = 0

Query: 1   MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60
           MA+L P DSF  S+  +DAGAAFVLESKG+WWHAGFHLTTAIVGPPILTLPFAFRGLGW 
Sbjct: 1   MADLSPPDSF--SKLSSDAGAAFVLESKGQWWHAGFHLTTAIVGPPILTLPFAFRGLGWG 60

Query: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120
           +GFLCLT MAAVTFYSYYLLSKVLE CEK GRRHIRFRELAAD+LGSGWMLYFVVFIQAA
Sbjct: 61  VGFLCLTVMAAVTFYSYYLLSKVLELCEKQGRRHIRFRELAADVLGSGWMLYFVVFIQAA 120

Query: 121 VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF 180
           VNTGVGVAAILLGGEC+ELMY  +YPKGELKLYHFIA+V++GMI+ISQ+P+FHSLR+INF
Sbjct: 121 VNTGVGVAAILLGGECLELMYSNIYPKGELKLYHFIAVVTLGMIIISQLPSFHSLRYINF 180

Query: 181 ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL 240
            SLLLSLAY FFIA ASILAGTSD VPPRDY LEST S RVFSAFTSISIFAAIFGNGIL
Sbjct: 181 LSLLLSLAYAFFIAFASILAGTSDNVPPRDYSLESTPSARVFSAFTSISIFAAIFGNGIL 240

Query: 241 PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP 300
           PEIQATLAPP+ GKMVKGLIMCYIVIFITFYSSAASGYWVFGN+SNSN+LKNLLPKNE P
Sbjct: 241 PEIQATLAPPIGGKMVKGLIMCYIVIFITFYSSAASGYWVFGNKSNSNILKNLLPKNEPP 300

Query: 301 LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY 360
           LAPTW+L LAVLFILLQLLAIGMVYAQVAYEI+ERRS DA+QGVFSRRNLIPRLILRTLY
Sbjct: 301 LAPTWILALAVLFILLQLLAIGMVYAQVAYEIMERRSADAKQGVFSRRNLIPRLILRTLY 360

Query: 361 MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIV 420
           M++CGF AAM PFFGDINSVVGAIGFIPLDF+LPMVLYNITHKPPV+ S+TYWVNVFI+ 
Sbjct: 361 MSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPPVT-SITYWVNVFIVA 420

Query: 421 AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 453
           AFSG G++GCFAS+RNLVLD+KKF LFSS VV
Sbjct: 421 AFSGVGLLGCFASIRNLVLDSKKFNLFSSHVV 449

BLAST of MS016288.1 vs. NCBI nr
Match: XP_038906705.1 (probable GABA transporter 2 [Benincasa hispida])

HSP 1 Score: 768.5 bits (1983), Expect = 3.3e-218
Identity = 391/452 (86.50%), Postives = 421/452 (93.14%), Query Frame = 0

Query: 1   MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60
           MA+L P DSF  S+  +DAGAAFVLESKG+WWHAGFHLTTAIVGPPILTLPFAFRGLGW 
Sbjct: 1   MADLSPPDSF--SKLSSDAGAAFVLESKGQWWHAGFHLTTAIVGPPILTLPFAFRGLGWG 60

Query: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120
           +GF+CLT MAAVTFYSYYLLSKVLE CEK GRRHIRFRELAAD+LGSGWMLY VVFIQAA
Sbjct: 61  VGFVCLTVMAAVTFYSYYLLSKVLELCEKQGRRHIRFRELAADVLGSGWMLYIVVFIQAA 120

Query: 121 VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF 180
           VNTGVGVAAILLGGEC+ELMY  +YPKGELKLYHFIAMV++GMI++SQ+P+FHSLR+INF
Sbjct: 121 VNTGVGVAAILLGGECLELMYSNIYPKGELKLYHFIAMVTMGMIIMSQLPSFHSLRYINF 180

Query: 181 ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL 240
            SLLLSLAY FFIA ASILAGTSD VPPRDY LESTLS RVFSAFTSISIFAAIFGNGIL
Sbjct: 181 LSLLLSLAYAFFIAFASILAGTSDNVPPRDYSLESTLSARVFSAFTSISIFAAIFGNGIL 240

Query: 241 PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP 300
           PEIQATLAPPV GKMVKGLIMCYIVIFITFYSSAASGYWVFGN SNSN+LKNLLPKNESP
Sbjct: 241 PEIQATLAPPVGGKMVKGLIMCYIVIFITFYSSAASGYWVFGNNSNSNILKNLLPKNESP 300

Query: 301 LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY 360
           LAPTW+L LAVLFILLQLLAIGMVYAQVAYEI+ERRS DA+QGVFSRRNLIPRLILRTLY
Sbjct: 301 LAPTWILLLAVLFILLQLLAIGMVYAQVAYEIMERRSADAKQGVFSRRNLIPRLILRTLY 360

Query: 361 MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIV 420
           M++CG  AAM PFFGDINSVVGAIGFIPLDF+LPMVLYNITHKPPV+ S+TYWVN+FI+ 
Sbjct: 361 MSLCGLFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPPVT-SITYWVNLFIVA 420

Query: 421 AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 453
           AFSG G++GCFAS RNLVLD+KKFKLFSS VV
Sbjct: 421 AFSGVGLLGCFASFRNLVLDSKKFKLFSSHVV 449

BLAST of MS016288.1 vs. NCBI nr
Match: KAA0042692.1 (putative GABA transporter 2 [Cucumis melo var. makuwa])

HSP 1 Score: 766.9 bits (1979), Expect = 9.6e-218
Identity = 394/453 (86.98%), Postives = 421/453 (92.94%), Query Frame = 0

Query: 1   MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60
           M +L P  SF  S+   DAGAAFVLESKG+WWHAGFHLTTAIVGPPILTLPFAFRGLGW 
Sbjct: 1   MTDLSPPASF--SKLSTDAGAAFVLESKGQWWHAGFHLTTAIVGPPILTLPFAFRGLGWG 60

Query: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120
           +GFLCLT MAAVTFYSYYLLSKVLE CEK GRRHIRFRELAAD+LGSGWMLYFVVFIQAA
Sbjct: 61  VGFLCLTVMAAVTFYSYYLLSKVLELCEKQGRRHIRFRELAADVLGSGWMLYFVVFIQAA 120

Query: 121 VNTGVGVAAILLGGECME-LMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHIN 180
           VNTGVGVAAILLGGEC+E LMY  +YPKGELKLYHFIA+V+VGMIMISQ+P+FHSLR+IN
Sbjct: 121 VNTGVGVAAILLGGECLEKLMYSNIYPKGELKLYHFIAVVTVGMIMISQLPSFHSLRYIN 180

Query: 181 FASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGI 240
           F SLLLSLAY FFIA ASILAGTSD VPPRDY LEST S RVFSAFTSISIFAAIFGNGI
Sbjct: 181 FLSLLLSLAYAFFIAFASILAGTSDNVPPRDYSLESTPSARVFSAFTSISIFAAIFGNGI 240

Query: 241 LPEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNES 300
           LPEIQATLAPP  GKMVKGLIMCYIVIFITFYSSAASGYWVFGN+SNSN+LKNLLPKNES
Sbjct: 241 LPEIQATLAPPTGGKMVKGLIMCYIVIFITFYSSAASGYWVFGNKSNSNILKNLLPKNES 300

Query: 301 PLAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTL 360
           PLAPTW+L LAVLFILLQLLAIGMVYAQVAYEI+ERRS DA+QGVFSRRNLIPRLILRTL
Sbjct: 301 PLAPTWILALAVLFILLQLLAIGMVYAQVAYEIMERRSADAKQGVFSRRNLIPRLILRTL 360

Query: 361 YMAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFII 420
           YM++CGF AAM PFFGDINSVVGAIGFIPLDF+LPMVLYNITHKPPV+ S+TYWVNVFI+
Sbjct: 361 YMSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPPVT-SITYWVNVFIV 420

Query: 421 VAFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 453
            AFSGAG++GCFAS+RNLVLD+KKFKLFSS VV
Sbjct: 421 AAFSGAGLLGCFASIRNLVLDSKKFKLFSSHVV 450

BLAST of MS016288.1 vs. ExPASy Swiss-Prot
Match: Q8L4X4 (Probable GABA transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g41800 PE=1 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 2.8e-180
Identity = 314/452 (69.47%), Postives = 377/452 (83.41%), Query Frame = 0

Query: 1   MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60
           M + P  D FP +R  +DAGA FVL+SKGEWWHAGFHLTTAIVGP ILTLP+AFRGLGW 
Sbjct: 1   MTDPPRPDPFPVTRLDSDAGALFVLQSKGEWWHAGFHLTTAIVGPTILTLPYAFRGLGWW 60

Query: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120
           LGF+CLT M  VTFY+YYL+SKVL+HCEK GRRHIRFRELAAD+LGSG M Y V+FIQ A
Sbjct: 61  LGFVCLTTMGLVTFYAYYLMSKVLDHCEKSGRRHIRFRELAADVLGSGLMFYVVIFIQTA 120

Query: 121 VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF 180
           +NTG+G+ AILL G+C+++MY  L+P+G LKLY FIAMV+V M+++SQ+P+FHSLRHIN 
Sbjct: 121 INTGIGIGAILLAGQCLDIMYSSLFPQGTLKLYEFIAMVTVVMMVLSQLPSFHSLRHINC 180

Query: 181 ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL 240
           ASLLLSL YTF +  A I  G S   P R+Y LE + S +VFSAFTSISI AAIFGNGIL
Sbjct: 181 ASLLLSLGYTFLVVGACINLGLSKNAPKREYSLEHSDSGKVFSAFTSISIIAAIFGNGIL 240

Query: 241 PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP 300
           PEIQATLAPP  GKM+KGL++CY VIF TFYS+A SGYWVFGN S+SN+LKNL+P     
Sbjct: 241 PEIQATLAPPATGKMLKGLLLCYSVIFFTFYSAAISGYWVFGNNSSSNILKNLMPDEGPT 300

Query: 301 LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY 360
           LAP  ++ LAV+F+LLQL AIG+VY+QVAYEI+E++S D  +G+FS+RNL+PRLILRTLY
Sbjct: 301 LAPIVVIGLAVIFVLLQLFAIGLVYSQVAYEIMEKKSADTTKGIFSKRNLVPRLILRTLY 360

Query: 361 MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIV 420
           MA CGF+AAMLPFFGDIN+VVGA GFIPLDFVLPM+LYN+T+K P   S TYW+N+ I+V
Sbjct: 361 MAFCGFMAAMLPFFGDINAVVGAFGFIPLDFVLPMLLYNMTYK-PTRRSFTYWINMTIMV 420

Query: 421 AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 453
            F+ AG+MG F+S+R LVLDA KFKLFSS+VV
Sbjct: 421 VFTCAGLMGAFSSIRKLVLDANKFKLFSSEVV 451

BLAST of MS016288.1 vs. ExPASy Swiss-Prot
Match: F4HW02 (GABA transporter 1 OS=Arabidopsis thaliana OX=3702 GN=GAT1 PE=1 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.3e-113
Identity = 210/432 (48.61%), Postives = 290/432 (67.13%), Query Frame = 0

Query: 18  DAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWILGFLCLTAMAAVTFYSY 77
           DAG+ FVL+SKG WWH GFHLTT+IV P +L+LP+AF+ LGW  G  CL   AAVTFYSY
Sbjct: 19  DAGSLFVLKSKGTWWHCGFHLTTSIVAPALLSLPYAFKFLGWAAGISCLVGGAAVTFYSY 78

Query: 78  YLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAAVNTGVGVAAILLGGECM 137
            LLS  LEH    G R++RFR++A  +L   W  Y+V  IQ AV  GV +A  LLGG+C+
Sbjct: 79  TLLSLTLEHHASLGNRYLRFRDMAHHILSPKWGRYYVGPIQMAVCYGVVIANALLGGQCL 138

Query: 138 ELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINFASLLLSLAYTFFIASAS 197
           + MY  + P GE+KL+ F+ +    +++++Q P+FHSLR+IN  SLLL L Y+   A+AS
Sbjct: 139 KAMYLVVQPNGEMKLFEFVIIFGCLLLVLAQFPSFHSLRYINSLSLLLCLLYSASAAAAS 198

Query: 198 ILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVK 257
           I  G     P +DY +      RVF  F +++I A  +GNGI+PEIQAT++ PV GKM+K
Sbjct: 199 IYIGKEPNAPEKDYTIVGDPETRVFGIFNAMAIIATTYGNGIIPEIQATISAPVKGKMMK 258

Query: 258 GLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLL-PKNESPLAPTWLLFLAVLFILL 317
           GL MCY+V+ +TF++ A +GYW FG ++N  +  N L  +      PTW +FL  LF +L
Sbjct: 259 GLCMCYLVVIMTFFTVAITGYWAFGKKANGLIFTNFLNAETNHYFVPTWFIFLVNLFTVL 318

Query: 318 QLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLYMAMCGFLAAMLPFFGD 377
           QL A+ +VY Q   +I+E    D  +  FS RN+IPRL++R+L++ M   +AAMLPFFGD
Sbjct: 319 QLSAVAVVYLQPINDILESVISDPTKKEFSIRNVIPRLVVRSLFVVMATIVAAMLPFFGD 378

Query: 378 INSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIVAFSGAGIMGCFASVRN 437
           +NS++GA GFIPLDFVLP+V +N T KP    S  +W+N  I V FS  G++   A+VR 
Sbjct: 379 VNSLLGAFGFIPLDFVLPVVFFNFTFKPSKK-SFIFWINTVIAVVFSCLGVIAMVAAVRQ 438

Query: 438 LVLDAKKFKLFS 449
           +++DA  +KLF+
Sbjct: 439 IIIDANTYKLFA 449

BLAST of MS016288.1 vs. ExPASy Swiss-Prot
Match: Q60DN5 (Proline transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PROT1 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.8e-38
Identity = 132/427 (30.91%), Postives = 207/427 (48.48%), Query Frame = 0

Query: 31  WWHAGFHLTTAIVGPPILTLPFAFR-GLGWILGFLCLTAMAAVTFYSYYLLSKVLEHCEK 90
           W+  GF LTT +    +L    +    LGWI G   L   AA++ Y+  LL+ +    E 
Sbjct: 66  WYQVGFILTTGVNSAYVLGYSASIMVPLGWIGGTCGLILAAAISMYANALLAHL---HEV 125

Query: 91  HGRRHIRFRELAADLLGS-----GWMLYFVVFIQAAVNTGVGVAAILLGGECMELMYWRL 150
            G+RHIR+R+LA  + G       W L +V      +NTG+    I+L G+ ++ +Y   
Sbjct: 126 GGKRHIRYRDLAGHIYGRKMYSLTWALQYVNLFM--INTGL----IILAGQALKAIYVLF 185

Query: 151 YPKGELKLYHFIAMVS-VGMIMISQIPTFHSLRHINFASLLLSLAYTFFIASASILAGTS 210
              G LKL + IA+   V  +    IP   +LR     S + SL Y       S+  G +
Sbjct: 186 RDDGVLKLPYCIALSGFVCALFAFGIPYLSALRIWLGLSTVFSLIYIMIAFVMSLRDGIT 245

Query: 211 DKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCY 270
              P +DY +  + S+R+F+   +++     +  G+LPEIQAT+ PPV   M K L   +
Sbjct: 246 --TPAKDYTIPGSHSDRIFTTIGAVANLVFAYNTGMLPEIQATIRPPVVKNMEKALWFQF 305

Query: 271 IVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESPLAPTWLLFLAVLFILLQLLAIGM 330
            V  +  Y+    GYW +G+ ++S LL        S   P W+  +A L   LQ +    
Sbjct: 306 TVGSLPLYAVTFMGYWAYGSSTSSYLL-------NSVKGPIWIKTVANLSAFLQTVIALH 365

Query: 331 VYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLYMAMCGFLAAMLPFFGDINSVVGA 390
           ++A   YE ++ R G    G F+  N++ R+ +R  Y+ +   +AAMLPF GD  S+ GA
Sbjct: 366 IFASPMYEFLDTRFGSGHGGPFAIHNIMFRVGVRGGYLTVNTLVAAMLPFLGDFMSLTGA 425

Query: 391 IGFIPLDFVLPMVLYNIT--HKPPVSWSVTYWVNVFIIVAFSGAGIMGCFASVRNLVLDA 449
           +   PL FVL   +Y     +K  +     +W+NV   V FS   +    A+VR + +D 
Sbjct: 426 LSTFPLTFVLANHMYLTVKQNKMSIFRKCWHWLNV---VGFSCLSVAAAVAAVRLITVDY 471

BLAST of MS016288.1 vs. ExPASy Swiss-Prot
Match: Q9FKS8 (Lysine histidine transporter 1 OS=Arabidopsis thaliana OX=3702 GN=LHT1 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.1e-38
Identity = 118/429 (27.51%), Postives = 215/429 (50.12%), Query Frame = 0

Query: 30  EWWHAGFHLTTAIVGPPILTLPFAFRGLGWILGFLCLTAMAAVTFYSYYLLSKVLEHCEK 89
           +WW++ FH  TA+VG  +L LP+A   LGW  G   L     +T Y+ + + ++  H   
Sbjct: 37  KWWYSAFHNVTAMVGAGVLGLPYAMSQLGWGPGIAVLVLSWVITLYTLWQMVEM--HEMV 96

Query: 90  HGRRHIRFRELAADLLGSGWMLYFVVFIQAAVNTGVGVAAILLGGECMELMYWRLYPKGE 149
            G+R  R+ EL     G    LY VV  Q  V  GV +  ++ GG+ ++  +  +    +
Sbjct: 97  PGKRFDRYHELGQHAFGEKLGLYIVVPQQLIVEIGVCIVYMVTGGKSLKKFHELVCDDCK 156

Query: 150 -LKLYHFIAMVSVGMIMISQIPTFHSLRHINFASLLLSLAYTFFIASASILAGTSDKVPP 209
            +KL +FI + +    ++S +P F+S+  ++ A+ ++SL+Y+    ++S   G  + V  
Sbjct: 157 PIKLTYFIMIFASVHFVLSHLPNFNSISGVSLAAAVMSLSYSTIAWASSASKGVQEDV-- 216

Query: 210 RDYHLESTLSERVFSAFTSISIFA-AIFGNGILPEIQATL----APPVAGKMVKGLIMCY 269
           +  +   T +  VF+ F+ +   A A  G+ ++ EIQAT+      P  G M +G+I+ Y
Sbjct: 217 QYGYKAKTTAGTVFNFFSGLGDVAFAYAGHNVVLEIQATIPSTPEKPSKGPMWRGVIVAY 276

Query: 270 IVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESPLAPTWLLFLAVLFILLQLLAIGM 329
           IV+ + ++  A  GY++FGN    N+L +L         P WL+  A +F+++ ++    
Sbjct: 277 IVVALCYFPVALVGYYIFGNGVEDNILMSL-------KKPAWLIATANIFVVIHVIGSYQ 336

Query: 330 VYAQVAYEIIERRSGDAQQGVFSRRNLIP----RLILRTLYMAMCGFLAAMLPFFGDINS 389
           +YA   ++++E         +  + N  P    R  +R  Y+A   F+    PFFG + +
Sbjct: 337 IYAMPVFDMMETL-------LVKKLNFRPTTTLRFFVRNFYVAATMFVGMTFPFFGGLLA 396

Query: 390 VVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIVAFSGAGIMGCFASVRNLVL 449
             G   F P  + LP V++   +KP   +S+++W N   IV      ++     +R +V+
Sbjct: 397 FFGGFAFAPTTYFLPCVIWLAIYKPK-KYSLSWWANWVCIVFGLFLMVLSPIGGLRTIVI 446

BLAST of MS016288.1 vs. ExPASy Swiss-Prot
Match: Q9C9J0 (Lysine histidine transporter-like 5 OS=Arabidopsis thaliana OX=3702 GN=At1g71680 PE=2 SV=2)

HSP 1 Score: 160.6 bits (405), Expect = 4.1e-38
Identity = 123/428 (28.74%), Postives = 216/428 (50.47%), Query Frame = 0

Query: 28  KGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWILGFLCLTAMAAVTFYSYYLLSKVLEHC 87
           + +W+++ FH  TA+VG  +L LPFA   LGW  G + +    A+TFYS + + ++  H 
Sbjct: 36  EAKWYYSAFHNVTAMVGAGVLGLPFAMSQLGWGPGLVAIIMSWAITFYSLWQMVQL--HE 95

Query: 88  EKHGRRHIRFRELAADLLGSGWMLYFVVFIQAAVNTGVGVAAILLGGECMELMYWRLYPK 147
              G+R  R+ EL  +  G     + V+  Q  V     +   + GG+ ++     L+P 
Sbjct: 96  AVPGKRLDRYPELGQEAFGPKLGYWIVMPQQLLVQIASDIVYNVTGGKSLKKFVELLFPN 155

Query: 148 GE-LKLYHFIAMVSVGMIMISQIPTFHSLRHINFASLLLSLAYTFFIASASILAGTSDKV 207
            E ++  ++I   +   +++SQ P F+S++ ++  + L+S  Y+   + ASI  GT  + 
Sbjct: 156 LEHIRQTYYILGFAALQLVLSQSPDFNSIKIVSLLAALMSFLYSMIASVASIAKGTEHR- 215

Query: 208 PPRDYHLE-STLSERVFSAFTSISIFAAIF-GNGILPEIQATL--APPVAGK--MVKGLI 267
            P  Y +   T++  VF AF  I   A  F G+ ++ EIQAT+   P V  K  M KG++
Sbjct: 216 -PSTYGVRGDTVASMVFDAFNGIGTIAFAFAGHSVVLEIQATIPSTPEVPSKKPMWKGVV 275

Query: 268 MCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESPLAPTWLLFLAVLFILLQLLA 327
           + YI++ I +   A SGYW FG     ++L +L         P WL+  A   + + ++ 
Sbjct: 276 VAYIIVIICYLFVAISGYWAFGAHVEDDVLISL-------ERPAWLIAAANFMVFIHVIG 335

Query: 328 IGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLYMAMCGFLAAMLPFFGDINSV 387
              V+A + ++ IE  S   +   F+    + RL+ R+ Y+A+   +A  +PFFG +   
Sbjct: 336 SYQVFAMIVFDTIE--SYLVKTLKFTPSTTL-RLVARSTYVALICLVAVCIPFFGGLLGF 395

Query: 388 VGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIVAFSGAGIMGCFASVRNLVLD 447
            G + F    + LP +++ I  K P  +S  +W +   IV      I+     +R+++L 
Sbjct: 396 FGGLVFSSTSYFLPCIIWLIM-KRPKRFSAHWWCSWVAIVTGISIAILAPIGGMRHIILS 448

Query: 448 AKKFKLFS 449
           A+ +KLFS
Sbjct: 456 ARTYKLFS 448

BLAST of MS016288.1 vs. ExPASy TrEMBL
Match: A0A6J1CXZ4 (probable GABA transporter 2 OS=Momordica charantia OX=3673 GN=LOC111015240 PE=3 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 9.3e-251
Identity = 449/452 (99.34%), Postives = 449/452 (99.34%), Query Frame = 0

Query: 1   MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60
           MAELPPADSFPASR KNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI
Sbjct: 1   MAELPPADSFPASRRKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60

Query: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120
           LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA
Sbjct: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120

Query: 121 VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF 180
           VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF
Sbjct: 121 VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF 180

Query: 181 ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL 240
           ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL
Sbjct: 181 ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL 240

Query: 241 PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP 300
           PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP
Sbjct: 241 PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP 300

Query: 301 LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY 360
           LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY
Sbjct: 301 LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY 360

Query: 361 MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIV 420
           MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVS  VTYWVNVFIIV
Sbjct: 361 MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSSWVTYWVNVFIIV 420

Query: 421 AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 453
           AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV
Sbjct: 421 AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 452

BLAST of MS016288.1 vs. ExPASy TrEMBL
Match: A0A1S3ATJ4 (probable GABA transporter 2 OS=Cucumis melo OX=3656 GN=LOC103482813 PE=3 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 1.9e-219
Identity = 394/452 (87.17%), Postives = 421/452 (93.14%), Query Frame = 0

Query: 1   MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60
           M +L P  SF  S+   DAGAAFVLESKG+WWHAGFHLTTAIVGPPILTLPFAFRGLGW 
Sbjct: 1   MTDLSPPASF--SKLSTDAGAAFVLESKGQWWHAGFHLTTAIVGPPILTLPFAFRGLGWG 60

Query: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120
           +GFLCLT MAAVTFYSYYLLSKVLE CEK GRRHIRFRELAAD+LGSGWMLYFVVFIQAA
Sbjct: 61  VGFLCLTVMAAVTFYSYYLLSKVLELCEKQGRRHIRFRELAADVLGSGWMLYFVVFIQAA 120

Query: 121 VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF 180
           VNTGVGVAAILLGGEC+ELMY  +YPKGELKLYHFIA+V+VGMIMISQ+P+FHSLR+INF
Sbjct: 121 VNTGVGVAAILLGGECLELMYSNIYPKGELKLYHFIAVVTVGMIMISQLPSFHSLRYINF 180

Query: 181 ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL 240
            SLLLSLAY FFIA ASILAGTSD VPPRDY LEST S RVFSAFTSISIFAAIFGNGIL
Sbjct: 181 LSLLLSLAYAFFIAFASILAGTSDNVPPRDYSLESTPSARVFSAFTSISIFAAIFGNGIL 240

Query: 241 PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP 300
           PEIQATLAPP  GKMVKGLIMCYIVIFITFYSSAASGYWVFGN+SNSN+LKNLLPKNESP
Sbjct: 241 PEIQATLAPPTGGKMVKGLIMCYIVIFITFYSSAASGYWVFGNKSNSNILKNLLPKNESP 300

Query: 301 LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY 360
           LAPTW+L LAVLFILLQLLAIGMVYAQVAYEI+ERRS DA+QGVFSRRNLIPRLILRTLY
Sbjct: 301 LAPTWILALAVLFILLQLLAIGMVYAQVAYEIMERRSADAKQGVFSRRNLIPRLILRTLY 360

Query: 361 MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIV 420
           M++CGF AAM PFFGDINSVVGAIGFIPLDF+LPMVLYNITHKPPV+ S+TYWVNVFI+ 
Sbjct: 361 MSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPPVT-SITYWVNVFIVA 420

Query: 421 AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 453
           AFSGAG++GCFAS+RNLVLD+KKFKLFSS VV
Sbjct: 421 AFSGAGLLGCFASIRNLVLDSKKFKLFSSHVV 449

BLAST of MS016288.1 vs. ExPASy TrEMBL
Match: A0A0A0KK40 (Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G150970 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 7.2e-219
Identity = 391/452 (86.50%), Postives = 422/452 (93.36%), Query Frame = 0

Query: 1   MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60
           MA+L P DSF  S+  +DAGAAFVLESKG+WWHAGFHLTTAIVGPPILTLPFAFRGLGW 
Sbjct: 1   MADLSPPDSF--SKLSSDAGAAFVLESKGQWWHAGFHLTTAIVGPPILTLPFAFRGLGWG 60

Query: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120
           +GFLCLT MAAVTFYSYYLLSKVLE CEK GRRHIRFRELAAD+LGSGWMLYFVVFIQAA
Sbjct: 61  VGFLCLTVMAAVTFYSYYLLSKVLELCEKQGRRHIRFRELAADVLGSGWMLYFVVFIQAA 120

Query: 121 VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF 180
           VNTGVGVAAILLGGEC+ELMY  +YPKGELKLYHFIA+V++GMI+ISQ+P+FHSLR+INF
Sbjct: 121 VNTGVGVAAILLGGECLELMYSNIYPKGELKLYHFIAVVTLGMIIISQLPSFHSLRYINF 180

Query: 181 ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL 240
            SLLLSLAY FFIA ASILAGTSD VPPRDY LEST S RVFSAFTSISIFAAIFGNGIL
Sbjct: 181 LSLLLSLAYAFFIAFASILAGTSDNVPPRDYSLESTPSARVFSAFTSISIFAAIFGNGIL 240

Query: 241 PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP 300
           PEIQATLAPP+ GKMVKGLIMCYIVIFITFYSSAASGYWVFGN+SNSN+LKNLLPKNE P
Sbjct: 241 PEIQATLAPPIGGKMVKGLIMCYIVIFITFYSSAASGYWVFGNKSNSNILKNLLPKNEPP 300

Query: 301 LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY 360
           LAPTW+L LAVLFILLQLLAIGMVYAQVAYEI+ERRS DA+QGVFSRRNLIPRLILRTLY
Sbjct: 301 LAPTWILALAVLFILLQLLAIGMVYAQVAYEIMERRSADAKQGVFSRRNLIPRLILRTLY 360

Query: 361 MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIV 420
           M++CGF AAM PFFGDINSVVGAIGFIPLDF+LPMVLYNITHKPPV+ S+TYWVNVFI+ 
Sbjct: 361 MSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPPVT-SITYWVNVFIVA 420

Query: 421 AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 453
           AFSG G++GCFAS+RNLVLD+KKF LFSS VV
Sbjct: 421 AFSGVGLLGCFASIRNLVLDSKKFNLFSSHVV 449

BLAST of MS016288.1 vs. ExPASy TrEMBL
Match: A0A5A7TH10 (Putative GABA transporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G001900 PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 4.6e-218
Identity = 394/453 (86.98%), Postives = 421/453 (92.94%), Query Frame = 0

Query: 1   MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60
           M +L P  SF  S+   DAGAAFVLESKG+WWHAGFHLTTAIVGPPILTLPFAFRGLGW 
Sbjct: 1   MTDLSPPASF--SKLSTDAGAAFVLESKGQWWHAGFHLTTAIVGPPILTLPFAFRGLGWG 60

Query: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120
           +GFLCLT MAAVTFYSYYLLSKVLE CEK GRRHIRFRELAAD+LGSGWMLYFVVFIQAA
Sbjct: 61  VGFLCLTVMAAVTFYSYYLLSKVLELCEKQGRRHIRFRELAADVLGSGWMLYFVVFIQAA 120

Query: 121 VNTGVGVAAILLGGECME-LMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHIN 180
           VNTGVGVAAILLGGEC+E LMY  +YPKGELKLYHFIA+V+VGMIMISQ+P+FHSLR+IN
Sbjct: 121 VNTGVGVAAILLGGECLEKLMYSNIYPKGELKLYHFIAVVTVGMIMISQLPSFHSLRYIN 180

Query: 181 FASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGI 240
           F SLLLSLAY FFIA ASILAGTSD VPPRDY LEST S RVFSAFTSISIFAAIFGNGI
Sbjct: 181 FLSLLLSLAYAFFIAFASILAGTSDNVPPRDYSLESTPSARVFSAFTSISIFAAIFGNGI 240

Query: 241 LPEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNES 300
           LPEIQATLAPP  GKMVKGLIMCYIVIFITFYSSAASGYWVFGN+SNSN+LKNLLPKNES
Sbjct: 241 LPEIQATLAPPTGGKMVKGLIMCYIVIFITFYSSAASGYWVFGNKSNSNILKNLLPKNES 300

Query: 301 PLAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTL 360
           PLAPTW+L LAVLFILLQLLAIGMVYAQVAYEI+ERRS DA+QGVFSRRNLIPRLILRTL
Sbjct: 301 PLAPTWILALAVLFILLQLLAIGMVYAQVAYEIMERRSADAKQGVFSRRNLIPRLILRTL 360

Query: 361 YMAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFII 420
           YM++CGF AAM PFFGDINSVVGAIGFIPLDF+LPMVLYNITHKPPV+ S+TYWVNVFI+
Sbjct: 361 YMSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPPVT-SITYWVNVFIV 420

Query: 421 VAFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 453
            AFSGAG++GCFAS+RNLVLD+KKFKLFSS VV
Sbjct: 421 AAFSGAGLLGCFASIRNLVLDSKKFKLFSSHVV 450

BLAST of MS016288.1 vs. ExPASy TrEMBL
Match: A0A6J1ESG7 (probable GABA transporter 2 OS=Cucurbita moschata OX=3662 GN=LOC111435583 PE=3 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 8.7e-217
Identity = 388/452 (85.84%), Postives = 420/452 (92.92%), Query Frame = 0

Query: 1   MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60
           MAELPPA+S   S    DAGAAFVLESKG+WWHAGFHLTTAIVGPPILTLPFAFRGLGW 
Sbjct: 1   MAELPPAESI--SELSPDAGAAFVLESKGQWWHAGFHLTTAIVGPPILTLPFAFRGLGWG 60

Query: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120
           +GF+CLT MAAVTFYSYYLLSKVLE CEK GRRHIRFRELAAD+LGSGWMLYFVVFIQAA
Sbjct: 61  VGFVCLTIMAAVTFYSYYLLSKVLELCEKQGRRHIRFRELAADVLGSGWMLYFVVFIQAA 120

Query: 121 VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF 180
           VNTGVGVAAILLGGEC+ELMY  ++P+GELKLYHFIAMV+VGMI+ISQ+P+FHSLR+INF
Sbjct: 121 VNTGVGVAAILLGGECLELMYSNIHPEGELKLYHFIAMVTVGMILISQLPSFHSLRYINF 180

Query: 181 ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL 240
            SLLLSLAY F IA ASILAGTSD VP + Y LESTLS R+F+AFTSISIFAAIFGNGIL
Sbjct: 181 VSLLLSLAYAFLIAFASILAGTSDNVPSKGYSLESTLSARLFTAFTSISIFAAIFGNGIL 240

Query: 241 PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP 300
           PEIQATLAPPV GKMVKGLIMCYIVIFITFYSSAASGYWVFGN SNSN+LKNLLPKN+SP
Sbjct: 241 PEIQATLAPPVGGKMVKGLIMCYIVIFITFYSSAASGYWVFGNESNSNILKNLLPKNQSP 300

Query: 301 LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY 360
           LAPTW+L  AVLFILLQLLAIGMVYAQVAYEI+E+RS DA+QGVFSRRNLIPRLILRTLY
Sbjct: 301 LAPTWILLFAVLFILLQLLAIGMVYAQVAYEIMEKRSADAKQGVFSRRNLIPRLILRTLY 360

Query: 361 MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIV 420
           M++CGF AAM PFFGDINS+VGAIGFIPLDFVLPMVLYNITHKPPV+ S+TYWVNVFI+V
Sbjct: 361 MSLCGFFAAMFPFFGDINSIVGAIGFIPLDFVLPMVLYNITHKPPVT-SITYWVNVFIVV 420

Query: 421 AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 453
           AFSG G++GCFAS+RNLVLDAKKFKLFSS VV
Sbjct: 421 AFSGVGLLGCFASIRNLVLDAKKFKLFSSHVV 449

BLAST of MS016288.1 vs. TAIR 10
Match: AT5G41800.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 632.9 bits (1631), Expect = 2.0e-181
Identity = 314/452 (69.47%), Postives = 377/452 (83.41%), Query Frame = 0

Query: 1   MAELPPADSFPASRSKNDAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWI 60
           M + P  D FP +R  +DAGA FVL+SKGEWWHAGFHLTTAIVGP ILTLP+AFRGLGW 
Sbjct: 1   MTDPPRPDPFPVTRLDSDAGALFVLQSKGEWWHAGFHLTTAIVGPTILTLPYAFRGLGWW 60

Query: 61  LGFLCLTAMAAVTFYSYYLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAA 120
           LGF+CLT M  VTFY+YYL+SKVL+HCEK GRRHIRFRELAAD+LGSG M Y V+FIQ A
Sbjct: 61  LGFVCLTTMGLVTFYAYYLMSKVLDHCEKSGRRHIRFRELAADVLGSGLMFYVVIFIQTA 120

Query: 121 VNTGVGVAAILLGGECMELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINF 180
           +NTG+G+ AILL G+C+++MY  L+P+G LKLY FIAMV+V M+++SQ+P+FHSLRHIN 
Sbjct: 121 INTGIGIGAILLAGQCLDIMYSSLFPQGTLKLYEFIAMVTVVMMVLSQLPSFHSLRHINC 180

Query: 181 ASLLLSLAYTFFIASASILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGIL 240
           ASLLLSL YTF +  A I  G S   P R+Y LE + S +VFSAFTSISI AAIFGNGIL
Sbjct: 181 ASLLLSLGYTFLVVGACINLGLSKNAPKREYSLEHSDSGKVFSAFTSISIIAAIFGNGIL 240

Query: 241 PEIQATLAPPVAGKMVKGLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESP 300
           PEIQATLAPP  GKM+KGL++CY VIF TFYS+A SGYWVFGN S+SN+LKNL+P     
Sbjct: 241 PEIQATLAPPATGKMLKGLLLCYSVIFFTFYSAAISGYWVFGNNSSSNILKNLMPDEGPT 300

Query: 301 LAPTWLLFLAVLFILLQLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLY 360
           LAP  ++ LAV+F+LLQL AIG+VY+QVAYEI+E++S D  +G+FS+RNL+PRLILRTLY
Sbjct: 301 LAPIVVIGLAVIFVLLQLFAIGLVYSQVAYEIMEKKSADTTKGIFSKRNLVPRLILRTLY 360

Query: 361 MAMCGFLAAMLPFFGDINSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIV 420
           MA CGF+AAMLPFFGDIN+VVGA GFIPLDFVLPM+LYN+T+K P   S TYW+N+ I+V
Sbjct: 361 MAFCGFMAAMLPFFGDINAVVGAFGFIPLDFVLPMLLYNMTYK-PTRRSFTYWINMTIMV 420

Query: 421 AFSGAGIMGCFASVRNLVLDAKKFKLFSSDVV 453
            F+ AG+MG F+S+R LVLDA KFKLFSS+VV
Sbjct: 421 VFTCAGLMGAFSSIRKLVLDANKFKLFSSEVV 451

BLAST of MS016288.1 vs. TAIR 10
Match: AT1G08230.2 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 411.4 bits (1056), Expect = 9.5e-115
Identity = 210/432 (48.61%), Postives = 290/432 (67.13%), Query Frame = 0

Query: 18  DAGAAFVLESKGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWILGFLCLTAMAAVTFYSY 77
           DAG+ FVL+SKG WWH GFHLTT+IV P +L+LP+AF+ LGW  G  CL   AAVTFYSY
Sbjct: 19  DAGSLFVLKSKGTWWHCGFHLTTSIVAPALLSLPYAFKFLGWAAGISCLVGGAAVTFYSY 78

Query: 78  YLLSKVLEHCEKHGRRHIRFRELAADLLGSGWMLYFVVFIQAAVNTGVGVAAILLGGECM 137
            LLS  LEH    G R++RFR++A  +L   W  Y+V  IQ AV  GV +A  LLGG+C+
Sbjct: 79  TLLSLTLEHHASLGNRYLRFRDMAHHILSPKWGRYYVGPIQMAVCYGVVIANALLGGQCL 138

Query: 138 ELMYWRLYPKGELKLYHFIAMVSVGMIMISQIPTFHSLRHINFASLLLSLAYTFFIASAS 197
           + MY  + P GE+KL+ F+ +    +++++Q P+FHSLR+IN  SLLL L Y+   A+AS
Sbjct: 139 KAMYLVVQPNGEMKLFEFVIIFGCLLLVLAQFPSFHSLRYINSLSLLLCLLYSASAAAAS 198

Query: 198 ILAGTSDKVPPRDYHLESTLSERVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVK 257
           I  G     P +DY +      RVF  F +++I A  +GNGI+PEIQAT++ PV GKM+K
Sbjct: 199 IYIGKEPNAPEKDYTIVGDPETRVFGIFNAMAIIATTYGNGIIPEIQATISAPVKGKMMK 258

Query: 258 GLIMCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLL-PKNESPLAPTWLLFLAVLFILL 317
           GL MCY+V+ +TF++ A +GYW FG ++N  +  N L  +      PTW +FL  LF +L
Sbjct: 259 GLCMCYLVVIMTFFTVAITGYWAFGKKANGLIFTNFLNAETNHYFVPTWFIFLVNLFTVL 318

Query: 318 QLLAIGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLYMAMCGFLAAMLPFFGD 377
           QL A+ +VY Q   +I+E    D  +  FS RN+IPRL++R+L++ M   +AAMLPFFGD
Sbjct: 319 QLSAVAVVYLQPINDILESVISDPTKKEFSIRNVIPRLVVRSLFVVMATIVAAMLPFFGD 378

Query: 378 INSVVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIVAFSGAGIMGCFASVRN 437
           +NS++GA GFIPLDFVLP+V +N T KP    S  +W+N  I V FS  G++   A+VR 
Sbjct: 379 VNSLLGAFGFIPLDFVLPVVFFNFTFKPSKK-SFIFWINTVIAVVFSCLGVIAMVAAVRQ 438

Query: 438 LVLDAKKFKLFS 449
           +++DA  +KLF+
Sbjct: 439 IIIDANTYKLFA 449

BLAST of MS016288.1 vs. TAIR 10
Match: AT1G71680.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 160.6 bits (405), Expect = 2.9e-39
Identity = 123/428 (28.74%), Postives = 216/428 (50.47%), Query Frame = 0

Query: 28  KGEWWHAGFHLTTAIVGPPILTLPFAFRGLGWILGFLCLTAMAAVTFYSYYLLSKVLEHC 87
           + +W+++ FH  TA+VG  +L LPFA   LGW  G + +    A+TFYS + + ++  H 
Sbjct: 36  EAKWYYSAFHNVTAMVGAGVLGLPFAMSQLGWGPGLVAIIMSWAITFYSLWQMVQL--HE 95

Query: 88  EKHGRRHIRFRELAADLLGSGWMLYFVVFIQAAVNTGVGVAAILLGGECMELMYWRLYPK 147
              G+R  R+ EL  +  G     + V+  Q  V     +   + GG+ ++     L+P 
Sbjct: 96  AVPGKRLDRYPELGQEAFGPKLGYWIVMPQQLLVQIASDIVYNVTGGKSLKKFVELLFPN 155

Query: 148 GE-LKLYHFIAMVSVGMIMISQIPTFHSLRHINFASLLLSLAYTFFIASASILAGTSDKV 207
            E ++  ++I   +   +++SQ P F+S++ ++  + L+S  Y+   + ASI  GT  + 
Sbjct: 156 LEHIRQTYYILGFAALQLVLSQSPDFNSIKIVSLLAALMSFLYSMIASVASIAKGTEHR- 215

Query: 208 PPRDYHLE-STLSERVFSAFTSISIFAAIF-GNGILPEIQATL--APPVAGK--MVKGLI 267
            P  Y +   T++  VF AF  I   A  F G+ ++ EIQAT+   P V  K  M KG++
Sbjct: 216 -PSTYGVRGDTVASMVFDAFNGIGTIAFAFAGHSVVLEIQATIPSTPEVPSKKPMWKGVV 275

Query: 268 MCYIVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESPLAPTWLLFLAVLFILLQLLA 327
           + YI++ I +   A SGYW FG     ++L +L         P WL+  A   + + ++ 
Sbjct: 276 VAYIIVIICYLFVAISGYWAFGAHVEDDVLISL-------ERPAWLIAAANFMVFIHVIG 335

Query: 328 IGMVYAQVAYEIIERRSGDAQQGVFSRRNLIPRLILRTLYMAMCGFLAAMLPFFGDINSV 387
              V+A + ++ IE  S   +   F+    + RL+ R+ Y+A+   +A  +PFFG +   
Sbjct: 336 SYQVFAMIVFDTIE--SYLVKTLKFTPSTTL-RLVARSTYVALICLVAVCIPFFGGLLGF 395

Query: 388 VGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIVAFSGAGIMGCFASVRNLVLD 447
            G + F    + LP +++ I  K P  +S  +W +   IV      I+     +R+++L 
Sbjct: 396 FGGLVFSSTSYFLPCIIWLIM-KRPKRFSAHWWCSWVAIVTGISIAILAPIGGMRHIILS 448

Query: 448 AKKFKLFS 449
           A+ +KLFS
Sbjct: 456 ARTYKLFS 448

BLAST of MS016288.1 vs. TAIR 10
Match: AT5G40780.1 (lysine histidine transporter 1 )

HSP 1 Score: 160.6 bits (405), Expect = 2.9e-39
Identity = 118/429 (27.51%), Postives = 215/429 (50.12%), Query Frame = 0

Query: 30  EWWHAGFHLTTAIVGPPILTLPFAFRGLGWILGFLCLTAMAAVTFYSYYLLSKVLEHCEK 89
           +WW++ FH  TA+VG  +L LP+A   LGW  G   L     +T Y+ + + ++  H   
Sbjct: 37  KWWYSAFHNVTAMVGAGVLGLPYAMSQLGWGPGIAVLVLSWVITLYTLWQMVEM--HEMV 96

Query: 90  HGRRHIRFRELAADLLGSGWMLYFVVFIQAAVNTGVGVAAILLGGECMELMYWRLYPKGE 149
            G+R  R+ EL     G    LY VV  Q  V  GV +  ++ GG+ ++  +  +    +
Sbjct: 97  PGKRFDRYHELGQHAFGEKLGLYIVVPQQLIVEIGVCIVYMVTGGKSLKKFHELVCDDCK 156

Query: 150 -LKLYHFIAMVSVGMIMISQIPTFHSLRHINFASLLLSLAYTFFIASASILAGTSDKVPP 209
            +KL +FI + +    ++S +P F+S+  ++ A+ ++SL+Y+    ++S   G  + V  
Sbjct: 157 PIKLTYFIMIFASVHFVLSHLPNFNSISGVSLAAAVMSLSYSTIAWASSASKGVQEDV-- 216

Query: 210 RDYHLESTLSERVFSAFTSISIFA-AIFGNGILPEIQATL----APPVAGKMVKGLIMCY 269
           +  +   T +  VF+ F+ +   A A  G+ ++ EIQAT+      P  G M +G+I+ Y
Sbjct: 217 QYGYKAKTTAGTVFNFFSGLGDVAFAYAGHNVVLEIQATIPSTPEKPSKGPMWRGVIVAY 276

Query: 270 IVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESPLAPTWLLFLAVLFILLQLLAIGM 329
           IV+ + ++  A  GY++FGN    N+L +L         P WL+  A +F+++ ++    
Sbjct: 277 IVVALCYFPVALVGYYIFGNGVEDNILMSL-------KKPAWLIATANIFVVIHVIGSYQ 336

Query: 330 VYAQVAYEIIERRSGDAQQGVFSRRNLIP----RLILRTLYMAMCGFLAAMLPFFGDINS 389
           +YA   ++++E         +  + N  P    R  +R  Y+A   F+    PFFG + +
Sbjct: 337 IYAMPVFDMMETL-------LVKKLNFRPTTTLRFFVRNFYVAATMFVGMTFPFFGGLLA 396

Query: 390 VVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIVAFSGAGIMGCFASVRNLVL 449
             G   F P  + LP V++   +KP   +S+++W N   IV      ++     +R +V+
Sbjct: 397 FFGGFAFAPTTYFLPCVIWLAIYKPK-KYSLSWWANWVCIVFGLFLMVLSPIGGLRTIVI 446

BLAST of MS016288.1 vs. TAIR 10
Match: AT5G40780.2 (lysine histidine transporter 1 )

HSP 1 Score: 160.6 bits (405), Expect = 2.9e-39
Identity = 118/429 (27.51%), Postives = 215/429 (50.12%), Query Frame = 0

Query: 30  EWWHAGFHLTTAIVGPPILTLPFAFRGLGWILGFLCLTAMAAVTFYSYYLLSKVLEHCEK 89
           +WW++ FH  TA+VG  +L LP+A   LGW  G   L     +T Y+ + + ++  H   
Sbjct: 36  KWWYSAFHNVTAMVGAGVLGLPYAMSQLGWGPGIAVLVLSWVITLYTLWQMVEM--HEMV 95

Query: 90  HGRRHIRFRELAADLLGSGWMLYFVVFIQAAVNTGVGVAAILLGGECMELMYWRLYPKGE 149
            G+R  R+ EL     G    LY VV  Q  V  GV +  ++ GG+ ++  +  +    +
Sbjct: 96  PGKRFDRYHELGQHAFGEKLGLYIVVPQQLIVEIGVCIVYMVTGGKSLKKFHELVCDDCK 155

Query: 150 -LKLYHFIAMVSVGMIMISQIPTFHSLRHINFASLLLSLAYTFFIASASILAGTSDKVPP 209
            +KL +FI + +    ++S +P F+S+  ++ A+ ++SL+Y+    ++S   G  + V  
Sbjct: 156 PIKLTYFIMIFASVHFVLSHLPNFNSISGVSLAAAVMSLSYSTIAWASSASKGVQEDV-- 215

Query: 210 RDYHLESTLSERVFSAFTSISIFA-AIFGNGILPEIQATL----APPVAGKMVKGLIMCY 269
           +  +   T +  VF+ F+ +   A A  G+ ++ EIQAT+      P  G M +G+I+ Y
Sbjct: 216 QYGYKAKTTAGTVFNFFSGLGDVAFAYAGHNVVLEIQATIPSTPEKPSKGPMWRGVIVAY 275

Query: 270 IVIFITFYSSAASGYWVFGNRSNSNLLKNLLPKNESPLAPTWLLFLAVLFILLQLLAIGM 329
           IV+ + ++  A  GY++FGN    N+L +L         P WL+  A +F+++ ++    
Sbjct: 276 IVVALCYFPVALVGYYIFGNGVEDNILMSL-------KKPAWLIATANIFVVIHVIGSYQ 335

Query: 330 VYAQVAYEIIERRSGDAQQGVFSRRNLIP----RLILRTLYMAMCGFLAAMLPFFGDINS 389
           +YA   ++++E         +  + N  P    R  +R  Y+A   F+    PFFG + +
Sbjct: 336 IYAMPVFDMMETL-------LVKKLNFRPTTTLRFFVRNFYVAATMFVGMTFPFFGGLLA 395

Query: 390 VVGAIGFIPLDFVLPMVLYNITHKPPVSWSVTYWVNVFIIVAFSGAGIMGCFASVRNLVL 449
             G   F P  + LP V++   +KP   +S+++W N   IV      ++     +R +V+
Sbjct: 396 FFGGFAFAPTTYFLPCVIWLAIYKPK-KYSLSWWANWVCIVFGLFLMVLSPIGGLRTIVI 445

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145892.11.9e-25099.34probable GABA transporter 2 [Momordica charantia][more]
XP_008437380.13.9e-21987.17PREDICTED: probable GABA transporter 2 [Cucumis melo][more]
XP_004143875.11.5e-21886.50probable GABA transporter 2 [Cucumis sativus] >KGN50030.1 hypothetical protein C... [more]
XP_038906705.13.3e-21886.50probable GABA transporter 2 [Benincasa hispida][more]
KAA0042692.19.6e-21886.98putative GABA transporter 2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q8L4X42.8e-18069.47Probable GABA transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g41800 PE=1 SV... [more]
F4HW021.3e-11348.61GABA transporter 1 OS=Arabidopsis thaliana OX=3702 GN=GAT1 PE=1 SV=1[more]
Q60DN51.8e-3830.91Proline transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PROT1 PE=2 SV=... [more]
Q9FKS84.1e-3827.51Lysine histidine transporter 1 OS=Arabidopsis thaliana OX=3702 GN=LHT1 PE=1 SV=1[more]
Q9C9J04.1e-3828.74Lysine histidine transporter-like 5 OS=Arabidopsis thaliana OX=3702 GN=At1g71680... [more]
Match NameE-valueIdentityDescription
A0A6J1CXZ49.3e-25199.34probable GABA transporter 2 OS=Momordica charantia OX=3673 GN=LOC111015240 PE=3 ... [more]
A0A1S3ATJ41.9e-21987.17probable GABA transporter 2 OS=Cucumis melo OX=3656 GN=LOC103482813 PE=3 SV=1[more]
A0A0A0KK407.2e-21986.50Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G150970 PE... [more]
A0A5A7TH104.6e-21886.98Putative GABA transporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A6J1ESG78.7e-21785.84probable GABA transporter 2 OS=Cucurbita moschata OX=3662 GN=LOC111435583 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G41800.12.0e-18169.47Transmembrane amino acid transporter family protein [more]
AT1G08230.29.5e-11548.61Transmembrane amino acid transporter family protein [more]
AT1G71680.12.9e-3928.74Transmembrane amino acid transporter family protein [more]
AT5G40780.12.9e-3927.51lysine histidine transporter 1 [more]
AT5G40780.22.9e-3927.51lysine histidine transporter 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013057Amino acid transporter, transmembrane domainPFAMPF01490Aa_transcoord: 28..435
e-value: 2.1E-58
score: 198.0
NoneNo IPR availablePANTHERPTHR48017:SF60GABA TRANSPORTER 2 ISOFORM X1-RELATEDcoord: 2..446
NoneNo IPR availablePANTHERPTHR48017OS05G0424000 PROTEIN-RELATEDcoord: 2..446

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MS016288MS016288gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MS016288.1-cdsMS016288.1-cds-scaffold1407:103550..103936CDS
MS016288.1-cdsMS016288.1-cds-scaffold1407:104014..104250CDS
MS016288.1-cdsMS016288.1-cds-scaffold1407:104718..104848CDS
MS016288.1-cdsMS016288.1-cds-scaffold1407:105592..105778CDS
MS016288.1-cdsMS016288.1-cds-scaffold1407:106238..106335CDS
MS016288.1-cdsMS016288.1-cds-scaffold1407:109225..109455CDS
MS016288.1-cdsMS016288.1-cds-scaffold1407:109546..109630CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MS016288.1MS016288.1-proteinpolypeptide