MS013206.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS013206.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Peptide chain release factor PrfB1
Location	scaffold459: 1194192 .. 1196930 (+)
Sequence length	1134
RNA-Seq Expression	MS013206.1
Synteny	MS013206.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GATTTTTATTCCTTAAGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGAAACTCTGCTGGGTTGTTTCAGTTGGATCAAGAACTTGCAGATTTGGAGTCGAAAGCGGCCGACAATGCTTTCTGGGATGACAGATCTAAGGCTCAACAAGTTCTTGTGGCCCTCACTGATGTGAAAGATAAAATAAAGATGCTTACTGATTTCAAAACACAGGTAGTGTATATAGCATGCAATTTAATCTTTAGAGAATACTCCATTTCATTTGCTAAAGTCACTTATGTAGACATATATGGAAGCCCTAAATTGAAAGAGTCTAAAATTATAGTAAATGATAAATGACTAATAAAGCCTTGGATCTTAGGCATCTACATCAATTTACATAATAATATACATGTTGTAACAATCCCCTCAAGTTGGAGCAAATATTAATCATGCCTAACTTGCTACAAAGCTACTCTATTCATACCCCATTTAAAGATTTTGTTATCAATATCTCCTGCTAGTTCTCCGATCTTTATATATCATGTAGACACCACTTCTATTTGCTTACAAGTAAAATGACAATTAACTTCCGTATATGTAGTTTGCATACATAAGTGCAAGTGTTTGTTGGATGGCTGTCTTAAGGTCATAGTTCACCTAAGTATAACATGGCTATGGCCTACTCTGTATTTTAGTTGGAATTGCAATATTTGATTTTAAGGCCTTTCCATTGGTCCTGTGGTCATGTAGAGAATGTTCTTTTTGTCTATTCATTTTCTTTTATGAGCAATACAATGGAAAAGGGGACTCTATACTTCTTTTTGGAACAGTACCCTTCAGAGGGTAGAATAAAGCTTGGAAGTAAGCTATTTTAAGATAATGGAACTTGAACTGTATATCTAGCTTGGAAGTAACCAAACTAATCCGTAGAAGCCAATTGGATAAATAGCCTATCCAAGTTTTTGAAAAGTGAATGAAATCATCATCAGAGGTGTGGGTTGCGATATGATTGATTCAGGTCGAAGAAGCAGAAACAATAGTTAAACTGACTGAAGAGATGGACTCTGTAGATACTGGGCTTCTTGAAGAAGCTGCTAGTATTATCAAAGACCTAAACAAGGCACTGGATCAATTTGAATTGACTCAACTTCTTTCTGGTCCGTATGACAAAGAAGGCGCTGTCATCACTATCTCGGCTGGAGCTGGGGGTACTGATGCACAGGTAAATAGTTAAGGTCTTATTGTGGAAACTGTTTTCAAAATAATTTTTTAGTTTTGTGTTCAAAACTGCTTCCAAGAAGTATGGTTGCGTGTGCATTTTTAAACTTCCAACTTTTTCACTTACTTCTTCGATATTAAATTTTTGATAATCTCCAGTGCAGGATTGGGCTGACATGCTTCTGAGGATGTATGTGAGATGGGGAGAGAAACAAAGATACAAAACAAGAGTGGTCGAGAAATCTGCAGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATCGAGGGTCGATATGCTTATGGATATATATCTGGAGAGAAAGGAACACACCGGATAGTGCGACAGTCTCCTTTTAACTCCAAAGGACTTCGACAGGTAATTATAACACTGCCCCCTGGTATCCCTACTACTTGGAGTTATCAAGGAAAGATGGACGATAGAGGGTGCATAATGTCATTATGTTTTAAACTTCTTATGAACTGTAAACTTAAGGTGGTTTTGAGCCTTCCATACCGAGTTTAGAATCTAGGGTCGACTTCTAATGTATGATGCTGCTTCCTTGGGATAGATTGTTTGATGGATGAACCTCTCTATTTTAATACGAGTTCTATGCAGACAAGTTTTTCTGGTGTGGAAGTCATGCCCCTCCTTCCCGACGAGTCCATGGATGTTGAATTACCAGAAGAGGACCTGGAAATAAGTTTTTCAAGGGCAGGAGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTTCGCATTACTCATATCCCAACAGGCGTCACGGTTCGCTGCACAGGTCTTCTATACTCATTTAAACATCATTGTTTATGCCACCATGAAAACTTGATGTCTGTTCTGTTCGTATGAGTTATTGTGTCCTCAATTTTTAATCAAAATTGTTCAAAGCCACCACCACTTTTAGCAGCATAATTTCTCCTCGAGTTGTTGATGGAAATGTAATGTTATCATGGTTGGAGTATTCATTTTCTCTGAGAATACATTTCATCCAAATGTTTCTGGAAATGTTTGGCATTTGCCTGCTACAATTTACCATCTTTAAAATCTTTGGATTAGATACACGCAACCCGGGACCATTCGTACCGAGTGGACCATATATGTTTTCATTGACAATCATTGACAACAAACCTTTCAAACGTCCCCATGTTGCATAGTTTTAAACTGACGGTATTTCATTCATCTTCTCAACTATAATGCAGAGGAGAGATCACAGCTAGCAAACAAGATCAAGGCGTTAAGCCGATTGAAGGCCAAACTCTTAGTCATAGCAGAGGAGCAACGTGCATCGGAAATCAAGCAAATACGAGGAGATGCCGTAAAGGCCGAGTGGGGGCAGCAAATAAGGAACTATGTGTTTCATCCATACAAGCTTGTGAAAGATGTTAGGACAGGATGCGAGACATCAGACATTGTCTCTGTAATGGATGGTGAATTGGAGCCCTTCATCAAAGCTTACCTGAAATACAAATACAGTATGGCATTGTCTACTGGTGGGGTTAAC

mRNA sequence

GATTTTTATTCCTTAAGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGAAACTCTGCTGGGTTGTTTCAGTTGGATCAAGAACTTGCAGATTTGGAGTCGAAAGCGGCCGACAATGCTTTCTGGGATGACAGATCTAAGGCTCAACAAGTTCTTGTGGCCCTCACTGATGTGAAAGATAAAATAAAGATGCTTACTGATTTCAAAACACAGGTAGTCGAAGAAGCAGAAACAATAGTTAAACTGACTGAAGAGATGGACTCTGTAGATACTGGGCTTCTTGAAGAAGCTGCTAGTATTATCAAAGACCTAAACAAGGCACTGGATCAATTTGAATTGACTCAACTTCTTTCTGGTCCGTATGACAAAGAAGGCGCTGTCATCACTATCTCGGCTGGAGCTGGGGGTACTGATGCACAGGATTGGGCTGACATGCTTCTGAGGATGTATGTGAGATGGGGAGAGAAACAAAGATACAAAACAAGAGTGGTCGAGAAATCTGCAGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATCGAGGGTCGATATGCTTATGGATATATATCTGGAGAGAAAGGAACACACCGGATAGTGCGACAGTCTCCTTTTAACTCCAAAGGACTTCGACAGACAAGTTTTTCTGGTGTGGAAGTCATGCCCCTCCTTCCCGACGAGTCCATGGATGTTGAATTACCAGAAGAGGACCTGGAAATAAGTTTTTCAAGGGCAGGAGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTTCGCATTACTCATATCCCAACAGGCGTCACGGTTCGCTGCACAGAGGAGAGATCACAGCTAGCAAACAAGATCAAGGCGTTAAGCCGATTGAAGGCCAAACTCTTAGTCATAGCAGAGGAGCAACGTGCATCGGAAATCAAGCAAATACGAGGAGATGCCGTAAAGGCCGAGTGGGGGCAGCAAATAAGGAACTATGTGTTTCATCCATACAAGCTTGTGAAAGATGTTAGGACAGGATGCGAGACATCAGACATTGTCTCTGTAATGGATGGTGAATTGGAGCCCTTCATCAAAGCTTACCTGAAATACAAATACAGTATGGCATTGTCTACTGGTGGGGTTAAC

Coding sequence (CDS)

GATTTTTATTCCTTAAGGAAAGAAGTGGAAACAACCTCAGAGCGTGTGGAAGAGATAAGAAACTCTGCTGGGTTGTTTCAGTTGGATCAAGAACTTGCAGATTTGGAGTCGAAAGCGGCCGACAATGCTTTCTGGGATGACAGATCTAAGGCTCAACAAGTTCTTGTGGCCCTCACTGATGTGAAAGATAAAATAAAGATGCTTACTGATTTCAAAACACAGGTAGTCGAAGAAGCAGAAACAATAGTTAAACTGACTGAAGAGATGGACTCTGTAGATACTGGGCTTCTTGAAGAAGCTGCTAGTATTATCAAAGACCTAAACAAGGCACTGGATCAATTTGAATTGACTCAACTTCTTTCTGGTCCGTATGACAAAGAAGGCGCTGTCATCACTATCTCGGCTGGAGCTGGGGGTACTGATGCACAGGATTGGGCTGACATGCTTCTGAGGATGTATGTGAGATGGGGAGAGAAACAAAGATACAAAACAAGAGTGGTCGAGAAATCTGCAGGAGAGGAAGCTGGAATAAAATCAGCTACAATTGAAATCGAGGGTCGATATGCTTATGGATATATATCTGGAGAGAAAGGAACACACCGGATAGTGCGACAGTCTCCTTTTAACTCCAAAGGACTTCGACAGACAAGTTTTTCTGGTGTGGAAGTCATGCCCCTCCTTCCCGACGAGTCCATGGATGTTGAATTACCAGAAGAGGACCTGGAAATAAGTTTTTCAAGGGCAGGAGGAAAAGGAGGTCAAAATGTTAACAAGGTTGAAACGGCTGTTCGCATTACTCATATCCCAACAGGCGTCACGGTTCGCTGCACAGAGGAGAGATCACAGCTAGCAAACAAGATCAAGGCGTTAAGCCGATTGAAGGCCAAACTCTTAGTCATAGCAGAGGAGCAACGTGCATCGGAAATCAAGCAAATACGAGGAGATGCCGTAAAGGCCGAGTGGGGGCAGCAAATAAGGAACTATGTGTTTCATCCATACAAGCTTGTGAAAGATGTTAGGACAGGATGCGAGACATCAGACATTGTCTCTGTAATGGATGGTGAATTGGAGCCCTTCATCAAAGCTTACCTGAAATACAAATACAGTATGGCATTGTCTACTGGTGGGGTTAAC

Protein sequence

DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTDVKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFIKAYLKYKYSMALSTGGVN

Homology

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BLAST of MS013206.1 vs. NCBI nr
Match: XP_022148293.1 (peptide chain release factor PrfB1, chloroplastic [Momordica charantia])

HSP 1 Score: 715.7 bits (1846), Expect = 2.1e-202
Identity = 374/378 (98.94%), Postives = 375/378 (99.21%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNA WDDRSKAQQVLVALTD
Sbjct: 82  DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNALWDDRSKAQQVLVALTD 141

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           VKDKIKMLTDFKTQ VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL
Sbjct: 142 VKDKIKMLTDFKTQ-VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 201

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA
Sbjct: 202 SGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 261

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED
Sbjct: 262 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 321

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI
Sbjct: 322 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 381

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDIVSVMDGELEPFI
Sbjct: 382 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFI 441

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLKYKYSMALSTGGVN
Sbjct: 442 KAYLKYKYSMALSTGGVN 458

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BLAST of MS013206.1 vs. NCBI nr
Match: KAG7036800.1 (Peptide chain release factor PrfB1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 704.9 bits (1818), Expect = 3.7e-199
Identity = 367/378 (97.09%), Postives = 373/378 (98.68%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+N+FWDDRSKAQQVL+ALTD
Sbjct: 75  DFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMALTD 134

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           VKDKIKMLTDFKTQ VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL
Sbjct: 135 VKDKIKMLTDFKTQ-VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 194

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA
Sbjct: 195 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 254

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVE+PEED
Sbjct: 255 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEED 314

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI
Sbjct: 315 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 374

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDIVSVMDGELEPFI
Sbjct: 375 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPFI 434

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLKYKYSMAL+TG VN
Sbjct: 435 KAYLKYKYSMALATGEVN 451

[Close]

BLAST of MS013206.1 vs. NCBI nr
Match: XP_022998733.1 (peptide chain release factor PrfB1, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 704.9 bits (1818), Expect = 3.7e-199
Identity = 367/378 (97.09%), Postives = 373/378 (98.68%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+N+FWDDRSKAQQVL+ALTD
Sbjct: 75  DFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMALTD 134

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           VKDKIKMLTDFKTQ VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL
Sbjct: 135 VKDKIKMLTDFKTQ-VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 194

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA
Sbjct: 195 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 254

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVE+PEED
Sbjct: 255 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEED 314

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI
Sbjct: 315 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 374

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDIVSVMDGELEPFI
Sbjct: 375 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPFI 434

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLKYKYSMAL+TG VN
Sbjct: 435 KAYLKYKYSMALATGEVN 451

[Close]

BLAST of MS013206.1 vs. NCBI nr
Match: XP_023525877.1 (peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 704.9 bits (1818), Expect = 3.7e-199
Identity = 367/378 (97.09%), Postives = 373/378 (98.68%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+N+FWDDRSKAQQVL+ALTD
Sbjct: 74  DFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMALTD 133

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           VKDKIKMLTDFKTQ VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL
Sbjct: 134 VKDKIKMLTDFKTQ-VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 193

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA
Sbjct: 194 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 253

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVE+PEED
Sbjct: 254 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEED 313

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI
Sbjct: 314 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 373

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDIVSVMDGELEPFI
Sbjct: 374 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPFI 433

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLKYKYSMAL+TG VN
Sbjct: 434 KAYLKYKYSMALATGEVN 450

[Close]

BLAST of MS013206.1 vs. NCBI nr
Match: XP_023525876.1 (peptide chain release factor PrfB1, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 704.9 bits (1818), Expect = 3.7e-199
Identity = 367/378 (97.09%), Postives = 373/378 (98.68%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+N+FWDDRSKAQQVL+ALTD
Sbjct: 75  DFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMALTD 134

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           VKDKIKMLTDFKTQ VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL
Sbjct: 135 VKDKIKMLTDFKTQ-VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 194

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA
Sbjct: 195 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 254

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVE+PEED
Sbjct: 255 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEED 314

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI
Sbjct: 315 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 374

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDIVSVMDGELEPFI
Sbjct: 375 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPFI 434

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLKYKYSMAL+TG VN
Sbjct: 435 KAYLKYKYSMALATGEVN 451

[Close]

BLAST of MS013206.1 vs. ExPASy Swiss-Prot
Match: Q9LVY0 (Peptide chain release factor PrfB1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PRFB1 PE=2 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 9.0e-172
Identity = 303/378 (80.16%), Postives = 348/378 (92.06%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFY+LRK+VE  S RVEEIR SA L QL+QE+ +LESKA D +FWDDR+KAQ+ L +L D
Sbjct: 80  DFYTLRKDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSLND 139

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           +KD++++L++FKT +VE+AETIVKLTEEMDS D  LLEEA  IIK+LNK+LD+FELTQLL
Sbjct: 140 LKDRMRLLSEFKT-MVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLL 199

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAV+ I+AGAGGTDAQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKSA
Sbjct: 200 SGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSA 259

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           T+EIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLP+E++ +E+PEED
Sbjct: 260 TLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEED 319

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           L+ISF+RAGGKGGQNVNKVETAVRITHIPTGV VRCTEERSQLANK +AL RLKAKL+VI
Sbjct: 320 LDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVI 379

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRA+EIK+IRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDI SVMDG+L+PFI
Sbjct: 380 AEEQRATEIKEIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPFI 439

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLK+KY++A+++   N
Sbjct: 440 KAYLKHKYTLAMASAVTN 456

[Close]

BLAST of MS013206.1 vs. ExPASy Swiss-Prot
Match: P74476 (Peptide chain release factor 2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=prfB PE=3 SV=2)

HSP 1 Score: 417.9 bits (1073), Expect = 1.2e-115
Identity = 214/362 (59.12%), Postives = 277/362 (76.52%), Query Frame = 0


Query: 5   LRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTDVKDK 64
           L++ +E  S R+ + ++   L  L  ++ DLE  AA   FWDD  +AQQ+L  L + K +
Sbjct: 8   LKRNLELISSRLGQTQDYLDLPGLKAKVQDLEQCAAQPDFWDDTDQAQQILQTLNETKSQ 67

Query: 65  IKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPY 124
           ++    ++ Q  ++++ IV+L E  D  D  LL EA + ++ L K LD++EL QLLSGPY
Sbjct: 68  LEQWGIWQQQ-WQDSQAIVELLELED--DQALLTEAETTLEQLQKELDRWELQQLLSGPY 127

Query: 125 DKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEI 184
           D +GA +TI+AGAGGTDAQDWA+MLLRMY RW EKQ YK  + E S G+EAG+KS T+EI
Sbjct: 128 DAKGATLTINAGAGGTDAQDWAEMLLRMYTRWSEKQGYKVHLAEISEGDEAGLKSVTLEI 187

Query: 185 EGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEIS 244
           EGRYAYGY+  EKGTHR+VR SPFN+ G RQTSF+GVEVMPLL +E++ +++P++DL+IS
Sbjct: 188 EGRYAYGYLKSEKGTHRLVRISPFNANGKRQTSFAGVEVMPLLGEEAISLDIPDKDLDIS 247

Query: 245 FSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQ 304
            SRAGGKGGQNVNKVETAVRI H+PTG+ VRCT+ERSQL NK KAL+ LKAKLL++ EEQ
Sbjct: 248 TSRAGGKGGQNVNKVETAVRIVHLPTGLAVRCTQERSQLQNKEKALAILKAKLLIVLEEQ 307

Query: 305 RASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFIKAYL 364
           RA  I +IRGD V+A WG QIRNYVFHPY+LVKD+RT  ET+D+  VMDGEL  FI+AYL
Sbjct: 308 RAQAIAEIRGDMVEAAWGTQIRNYVFHPYQLVKDLRTNVETTDVGGVMDGELSDFIEAYL 366

Query: 365 KY 367
           ++
Sbjct: 368 RH 366

[Close]

BLAST of MS013206.1 vs. ExPASy Swiss-Prot
Match: B7K0A6 (Peptide chain release factor 2 OS=Rippkaea orientalis (strain PCC 8801) OX=41431 GN=prfB PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 1.1e-113
Identity = 210/361 (58.17%), Postives = 276/361 (76.45%), Query Frame = 0


Query: 5   LRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTDVKDK 64
           L++E++  + R+ + ++   L  L+  + DLE  AA   FWD++  AQ+ L  L D+K  
Sbjct: 6   LKREIQQIAARLGKTQDYLDLPALNANIQDLEQIAAQPDFWDNQETAQKTLQQLNDLKSS 65

Query: 65  IKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPY 124
           ++    +  Q +E+ + I +L E  +  D  L EEA + +  LN  LD++EL +LLSG Y
Sbjct: 66  VEEYHQWMGQ-LEDLKAIAELLELEE--DATLNEEAEANLTQLNHELDRWELQRLLSGIY 125

Query: 125 DKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEI 184
           D +GAV+TI+AGAGGTDAQDWA+MLLRMY RWGE+Q YK  + E S G+EAGIKSAT+EI
Sbjct: 126 DSKGAVLTINAGAGGTDAQDWAEMLLRMYTRWGEQQGYKVHLTEISEGDEAGIKSATLEI 185

Query: 185 EGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEIS 244
           EGRYAYGY+ GEKGTHR+VR SPFN+ G RQTSF+G+EVMP L +E + VE+PE+DLEI+
Sbjct: 186 EGRYAYGYLKGEKGTHRLVRISPFNANGKRQTSFAGIEVMPALEEEDLKVEIPEKDLEIT 245

Query: 245 FSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQ 304
            +R+GGKGGQNVNKVETAVR+ H+PTG+ VRCT+ERSQL NK KAL+ LKAKLL+IA+EQ
Sbjct: 246 TTRSGGKGGQNVNKVETAVRVVHLPTGIAVRCTQERSQLQNKEKALALLKAKLLIIAQEQ 305

Query: 305 RASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFIKAYL 364
           RA  I +IRGD V+A WG QIRNYVFHPY++VKDVRT  ET+D+  VMDG+L+ FI++YL
Sbjct: 306 RAQAIAEIRGDMVEAAWGNQIRNYVFHPYQMVKDVRTNTETTDVNGVMDGKLDLFIESYL 363

Query: 365 K 366
           +
Sbjct: 366 R 363

[Close]

BLAST of MS013206.1 vs. ExPASy Swiss-Prot
Match: Q7U3W6 (Peptide chain release factor 2 OS=Synechococcus sp. (strain WH8102) OX=84588 GN=prfB PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.2e-107
Identity = 206/365 (56.44%), Postives = 267/365 (73.15%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           D    ++++   ++R+   ++   +  L     DLE  AA   FWDD+  AQ+ +  L +
Sbjct: 2   DLTDFKRDLSELTDRLGHAQDCLDVPALKARQQDLEQLAAQPDFWDDQQAAQKQMRRLDE 61

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           VK +++ L D+    V++A+  ++L E     D  +L EA   +  L + LD++EL +LL
Sbjct: 62  VKAQLQQLADW-GGAVDDAKATLELYEL--EPDEEMLTEAQEGLNQLRQGLDRWELERLL 121

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SG YDKEGAV+TI+AGAGGTDAQDWA MLLRMY RW E    K  V E S GEEAGIKS 
Sbjct: 122 SGDYDKEGAVLTINAGAGGTDAQDWAQMLLRMYTRWAEDHGMKVTVDELSEGEEAGIKSC 181

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           TIE+EGRYAYGY+  EKGTHR+VR SPFN+   RQTSF+G+EVMP + DE +D+++PE+D
Sbjct: 182 TIEVEGRYAYGYLRNEKGTHRLVRISPFNANDKRQTSFAGIEVMPKI-DEEVDIDIPEKD 241

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           LE++ SR+GG GGQNVNKVETAVRI HIPTG+ VRCT+ERSQL NK KA++ LKAKLLVI
Sbjct: 242 LEVTTSRSGGAGGQNVNKVETAVRILHIPTGLAVRCTQERSQLQNKEKAMALLKAKLLVI 301

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           A+EQRA+EI  IRGD V+A WG QIRNYVFHPY++VKD+RT  ET+D+ +VMDG L+PFI
Sbjct: 302 AQEQRAAEIADIRGDIVEAAWGNQIRNYVFHPYQMVKDLRTSEETNDVQAVMDGALDPFI 361

Query: 361 KAYLK 366
            A L+
Sbjct: 362 DASLR 362

[Close]

BLAST of MS013206.1 vs. ExPASy Swiss-Prot
Match: O67695 (Peptide chain release factor 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=prfB PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 5.2e-87
Identity = 167/363 (46.01%), Postives = 246/363 (67.77%), Query Frame = 0


Query: 5   LRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTDVKDK 64
           L+ +VE   +R+E+++      +L+ EL +L+ K ++  FW+D+ KA+QV+     V++ 
Sbjct: 6   LKGKVEELRKRLEDVKKILSPEKLESELKELDQKMSEPNFWEDQEKAKQVIQRRKWVEET 65

Query: 65  IKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPY 124
           +  L + + + V++ E +V++T E D+    +++E    IK++ + L + EL   LSG  
Sbjct: 66  LNKLKNLE-KSVKDLEELVEITSEEDTETWAMMDEE---IKEVERTLRELELKTYLSGEM 125

Query: 125 DKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEI 184
           D + A +TI AGAGGT+A DWADML RMY RW EK+ Y+  +++ +  + AGIKS T+ +
Sbjct: 126 DAKNAYLTIQAGAGGTEACDWADMLFRMYKRWAEKKGYEVELIDITPDDVAGIKSVTVLV 185

Query: 185 EGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEIS 244
           +G YAYGY+ GE+G HR+VR SPF++   R TSF+ V VMP + DE + +E+  EDL+I 
Sbjct: 186 KGPYAYGYLKGEQGVHRLVRISPFDANARRHTSFAAVSVMPQI-DEDIKIEIKPEDLKIE 245

Query: 245 FSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQ 304
             RA G GGQ VNK +TAVRITHIPTG+TV C +ERSQ  NK KAL  LKAKL  +  ++
Sbjct: 246 TFRASGAGGQYVNKTDTAVRITHIPTGITVSCQQERSQYQNKRKALELLKAKLYQLEMKK 305

Query: 305 RASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFIKAYL 364
              + KQ  G+     WG QIR+YVFHPYKL+KD+RTG ET ++ +VMDGE++ FI++YL
Sbjct: 306 LEEKKKQYEGEKTDIGWGHQIRSYVFHPYKLIKDLRTGYETGNVEAVMDGEIDEFIESYL 363

Query: 365 KYK 368
           K+K
Sbjct: 366 KWK 363

[Close]

BLAST of MS013206.1 vs. ExPASy TrEMBL
Match: A0A6J1D3Q2 (peptide chain release factor PrfB1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016981 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.0e-202
Identity = 374/378 (98.94%), Postives = 375/378 (99.21%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNA WDDRSKAQQVLVALTD
Sbjct: 82  DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNALWDDRSKAQQVLVALTD 141

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           VKDKIKMLTDFKTQ VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL
Sbjct: 142 VKDKIKMLTDFKTQ-VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 201

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAVI+ISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA
Sbjct: 202 SGPYDKEGAVISISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 261

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED
Sbjct: 262 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 321

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI
Sbjct: 322 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 381

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDIVSVMDGELEPFI
Sbjct: 382 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDIVSVMDGELEPFI 441

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLKYKYSMALSTGGVN
Sbjct: 442 KAYLKYKYSMALSTGGVN 458

[Close]

BLAST of MS013206.1 vs. ExPASy TrEMBL
Match: A0A6J1KF53 (peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493309 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.8e-199
Identity = 367/378 (97.09%), Postives = 373/378 (98.68%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+N+FWDDRSKAQQVL+ALTD
Sbjct: 74  DFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMALTD 133

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           VKDKIKMLTDFKTQ VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL
Sbjct: 134 VKDKIKMLTDFKTQ-VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 193

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA
Sbjct: 194 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 253

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVE+PEED
Sbjct: 254 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEED 313

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI
Sbjct: 314 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 373

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDIVSVMDGELEPFI
Sbjct: 374 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPFI 433

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLKYKYSMAL+TG VN
Sbjct: 434 KAYLKYKYSMALATGEVN 450

[Close]

BLAST of MS013206.1 vs. ExPASy TrEMBL
Match: A0A6J1KHL0 (peptide chain release factor PrfB1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493309 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.8e-199
Identity = 367/378 (97.09%), Postives = 373/378 (98.68%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+N+FWDDRSKAQQVL+ALTD
Sbjct: 75  DFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMALTD 134

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           VKDKIKMLTDFKTQ VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL
Sbjct: 135 VKDKIKMLTDFKTQ-VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 194

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA
Sbjct: 195 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 254

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVE+PEED
Sbjct: 255 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEED 314

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI
Sbjct: 315 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 374

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDIVSVMDGELEPFI
Sbjct: 375 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPFI 434

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLKYKYSMAL+TG VN
Sbjct: 435 KAYLKYKYSMALATGEVN 451

[Close]

BLAST of MS013206.1 vs. ExPASy TrEMBL
Match: A0A6J1GD28 (peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452850 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.8e-199
Identity = 367/378 (97.09%), Postives = 373/378 (98.68%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+N+FWDDRSKAQQVL+ALTD
Sbjct: 74  DFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMALTD 133

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           VKDKIKMLTDFKTQ VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL
Sbjct: 134 VKDKIKMLTDFKTQ-VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 193

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA
Sbjct: 194 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 253

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVE+PEED
Sbjct: 254 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEED 313

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI
Sbjct: 314 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 373

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDIVSVMDGELEPFI
Sbjct: 374 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPFI 433

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLKYKYSMAL+TG VN
Sbjct: 434 KAYLKYKYSMALATGEVN 450

[Close]

BLAST of MS013206.1 vs. ExPASy TrEMBL
Match: A0A6J1GCA2 (peptide chain release factor PrfB1, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452850 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.8e-199
Identity = 367/378 (97.09%), Postives = 373/378 (98.68%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFYSLRKEVET SERVEEIRNSAGL QLDQE+ADLESKAA+N+FWDDRSKAQQVL+ALTD
Sbjct: 75  DFYSLRKEVETASERVEEIRNSAGLLQLDQEVADLESKAAENSFWDDRSKAQQVLMALTD 134

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           VKDKIKMLTDFKTQ VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL
Sbjct: 135 VKDKIKMLTDFKTQ-VEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 194

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA
Sbjct: 195 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 254

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVE+PEED
Sbjct: 255 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVEIPEED 314

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI
Sbjct: 315 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 374

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDIVSVMDGELEPFI
Sbjct: 375 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIVSVMDGELEPFI 434

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLKYKYSMAL+TG VN
Sbjct: 435 KAYLKYKYSMALATGEVN 451

[Close]

BLAST of MS013206.1 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 604.4 bits (1557), Expect = 6.4e-173
Identity = 303/378 (80.16%), Postives = 348/378 (92.06%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFY+LRK+VE  S RVEEIR SA L QL+QE+ +LESKA D +FWDDR+KAQ+ L +L D
Sbjct: 80  DFYTLRKDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSLND 139

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           +KD++++L++FKT +VE+AETIVKLTEEMDS D  LLEEA  IIK+LNK+LD+FELTQLL
Sbjct: 140 LKDRMRLLSEFKT-MVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLL 199

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAV+ I+AGAGGTDAQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKSA
Sbjct: 200 SGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSA 259

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           T+EIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLP+E++ +E+PEED
Sbjct: 260 TLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEED 319

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           L+ISF+RAGGKGGQNVNKVETAVRITHIPTGV VRCTEERSQLANK +AL RLKAKL+VI
Sbjct: 320 LDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVI 379

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRA+EIK+IRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDI SVMDG+L+PFI
Sbjct: 380 AEEQRATEIKEIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPFI 439

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLK+KY++A+++   N
Sbjct: 440 KAYLKHKYTLAMASAVTN 456

[Close]

BLAST of MS013206.1 vs. TAIR 10
Match: AT5G36170.2 (high chlorophyll fluorescent 109 )

HSP 1 Score: 604.4 bits (1557), Expect = 6.4e-173
Identity = 303/378 (80.16%), Postives = 348/378 (92.06%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFY+LRK+VE  S RVEEIR SA L QL+QE+ +LESKA D +FWDDR+KAQ+ L +L D
Sbjct: 79  DFYTLRKDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSLND 138

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           +KD++++L++FKT +VE+AETIVKLTEEMDS D  LLEEA  IIK+LNK+LD+FELTQLL
Sbjct: 139 LKDRMRLLSEFKT-MVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLL 198

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAV+ I+AGAGGTDAQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKSA
Sbjct: 199 SGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSA 258

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           T+EIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLP+E++ +E+PEED
Sbjct: 259 TLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEED 318

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 300
           L+ISF+RAGGKGGQNVNKVETAVRITHIPTGV VRCTEERSQLANK +AL RLKAKL+VI
Sbjct: 319 LDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAKLMVI 378

Query: 301 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 360
           AEEQRA+EIK+IRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDI SVMDG+L+PFI
Sbjct: 379 AEEQRATEIKEIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDITSVMDGDLDPFI 438

Query: 361 KAYLKYKYSMALSTGGVN 379
           KAYLK+KY++A+++   N
Sbjct: 439 KAYLKHKYTLAMASAVTN 455

[Close]

BLAST of MS013206.1 vs. TAIR 10
Match: AT5G36170.3 (high chlorophyll fluorescent 109 )

HSP 1 Score: 441.0 bits (1133), Expect = 9.4e-124
Identity = 221/278 (79.50%), Postives = 255/278 (91.73%), Query Frame = 0


Query: 1   DFYSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTD 60
           DFY+LRK+VE  S RVEEIR SA L QL+QE+ +LESKA D +FWDDR+KAQ+ L +L D
Sbjct: 80  DFYTLRKDVEIASARVEEIRASANLQQLEQEITNLESKATDTSFWDDRTKAQETLSSLND 139

Query: 61  VKDKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLL 120
           +KD++++L++FKT +VE+AETIVKLTEEMDS D  LLEEA  IIK+LNK+LD+FELTQLL
Sbjct: 140 LKDRMRLLSEFKT-MVEDAETIVKLTEEMDSTDVSLLEEAMGIIKELNKSLDKFELTQLL 199

Query: 121 SGPYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSA 180
           SGPYDKEGAV+ I+AGAGGTDAQDWADMLLRMY+RWGEKQRYKT+VVE S GEEAGIKSA
Sbjct: 200 SGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKVVEMSNGEEAGIKSA 259

Query: 181 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 240
           T+EIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLP+E++ +E+PEED
Sbjct: 260 TLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEEAVGIEIPEED 319

Query: 241 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTE 279
           L+ISF+RAGGKGGQNVNKVETAVRITHIPTGV VRCT+
Sbjct: 320 LDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTD 356

[Close]

BLAST of MS013206.1 vs. TAIR 10
Match: AT1G56350.1 (Peptide chain release factor 2 )

HSP 1 Score: 241.9 bits (616), Expect = 8.4e-64
Identity = 134/352 (38.07%), Postives = 206/352 (58.52%), Query Frame = 0


Query: 13  SERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTDVKDKIKMLTDFK 72
           ++ ++ I+      +L   L  L ++   +  WDD + A ++      +  K+K +  F+
Sbjct: 123 AQSIQVIKRRLQWKKLLVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFE 182

Query: 73  TQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSGPYDKEGAVIT 132
            +++E  + +    EE DS    L  E    + D+ +   + EL  LLS   D     I 
Sbjct: 183 RELLEHIDMLKLAKEENDS---ELESETLKALIDMRRVSKEKELEALLSADNDPCSCYIE 242

Query: 133 ISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSAGEEAGIKSATIEIEGRYAYGY 192
           + AGAGGT++ DWA M++ MY  W +++++   VV+++ GE AGIK ATI++ G YAYGY
Sbjct: 243 VQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGY 302

Query: 193 ISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEEDLEISFSRAGGKG 252
              E G HR+VR SPF+S   R TSF+ V V+P+L D S  VE+ + DL I   R+GG G
Sbjct: 303 AKAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILGDGSTRVEINDSDLRIERFRSGGAG 362

Query: 253 GQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQI 312
           GQ+ N  ++AVRI HIPTG+T  C  ERSQ +NK  A++ L+++L  +   ++ +   Q 
Sbjct: 363 GQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQH 422

Query: 313 RGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFIKAYL 365
                +  WG QIR YV HPY++VKD+RT  E SD  SV++G+L+ FI ++L
Sbjct: 423 TQSLTEISWGNQIRTYVLHPYRMVKDLRTNYEVSDPDSVLEGDLDGFILSFL 471

[Close]

BLAST of MS013206.1 vs. TAIR 10
Match: AT3G57190.1 (peptide chain release factor, putative )

HSP 1 Score: 209.9 bits (533), Expect = 3.5e-54
Identity = 129/370 (34.86%), Postives = 218/370 (58.92%), Query Frame = 0


Query: 3   YSLRKEVETTSERVEEIRNSAGLFQLDQELADLESKAADNAFWDDRSKAQQVLVALTDVK 62
           +SL+K+++    + E     A   + +Q +   E+    +  WDD +K+ ++L+ L D  
Sbjct: 48  FSLKKKIKDVVLKAEMFAPDALELEEEQWIKQEETMRYFD-LWDDPAKSDEILLKLADRA 107

Query: 63  DKIKMLTDFKTQVVEEAETIVKLTEEMDSVDTGLLEEAASIIKDLNKALDQFELTQLLSG 122
             +  L D K +  EEA+ I++L  EMD++D  L E+A     D++++L  +E+++LL  
Sbjct: 108 KAVDSLKDLKYK-AEEAKLIIQL-GEMDAIDYSLFEQAYDSSLDVSRSLHHYEMSKLLRD 167

Query: 123 PYDKEGAVITISAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSA--GEEAGIKSA 182
            YD EGA + I +G+ G  +Q W + ++ MY++W E+     RV EK +    ++G+ SA
Sbjct: 168 QYDAEGACMIIKSGSPGAKSQIWTEQVVSMYIKWAERLGQNARVAEKCSLLSNKSGVSSA 227

Query: 183 TIEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPDESMDVELPEED 242
           TIE E  +AYGY+ GE+G HR++  S  N     +   + V+++PL    S D E+ E D
Sbjct: 228 TIEFEFEFAYGYLLGERGVHRLIISSTSN-----EECSATVDIIPLFLRASPDFEVKEGD 287

Query: 243 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVI 302
           L +S+        ++    E  V I HIP+GVT++ + ER++ AN+IKAL+RLKAKLLVI
Sbjct: 288 LIVSY-----PAKEDHKIAENMVCIHHIPSGVTLQSSGERNRFANRIKALNRLKAKLLVI 347

Query: 303 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGCETSDIVSVMDGELEPFI 362
           A+EQ+ S++ +I    +  E  ++ R+YV   +K+V D +TG E  D+ SV+DG + P +
Sbjct: 348 AKEQKVSDVNKIDSKNI-LEPREETRSYVSKGHKMVVDRKTGLEILDLKSVLDGNIGPLL 403

Query: 363 KAYLKYKYSM 371
            A++  + S+
Sbjct: 408 GAHISMRRSI 403

The following BLAST results are available for this feature:

BLAST of MS013206.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148293.1	2.1e-202	98.94	peptide chain release factor PrfB1, chloroplastic [Momordica charantia]	[more]
KAG7036800.1	3.7e-199	97.09	Peptide chain release factor PrfB1, chloroplastic [Cucurbita argyrosperma subsp....	[more]
XP_022998733.1	3.7e-199	97.09	peptide chain release factor PrfB1, chloroplastic isoform X1 [Cucurbita maxima]	[more]
XP_023525877.1	3.7e-199	97.09	peptide chain release factor PrfB1, chloroplastic isoform X2 [Cucurbita pepo sub...	[more]
XP_023525876.1	3.7e-199	97.09	peptide chain release factor PrfB1, chloroplastic isoform X1 [Cucurbita pepo sub...	[more]

BLAST of MS013206.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVY0	9.0e-172	80.16	Peptide chain release factor PrfB1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
P74476	1.2e-115	59.12	Peptide chain release factor 2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) O...	[more]
B7K0A6	1.1e-113	58.17	Peptide chain release factor 2 OS=Rippkaea orientalis (strain PCC 8801) OX=41431...	[more]
Q7U3W6	1.2e-107	56.44	Peptide chain release factor 2 OS=Synechococcus sp. (strain WH8102) OX=84588 GN=...	[more]
O67695	5.2e-87	46.01	Peptide chain release factor 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=prf...	[more]

BLAST of MS013206.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D3Q2	1.0e-202	98.94	peptide chain release factor PrfB1, chloroplastic OS=Momordica charantia OX=3673...	[more]
A0A6J1KF53	1.8e-199	97.09	peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita maxima...	[more]
A0A6J1KHL0	1.8e-199	97.09	peptide chain release factor PrfB1, chloroplastic isoform X1 OS=Cucurbita maxima...	[more]
A0A6J1GD28	1.8e-199	97.09	peptide chain release factor PrfB1, chloroplastic isoform X2 OS=Cucurbita moscha...	[more]
A0A6J1GCA2	1.8e-199	97.09	peptide chain release factor PrfB1, chloroplastic isoform X1 OS=Cucurbita moscha...	[more]

BLAST of MS013206.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36170.1	6.4e-173	80.16	high chlorophyll fluorescent 109	[more]
AT5G36170.2	6.4e-173	80.16	high chlorophyll fluorescent 109	[more]
AT5G36170.3	9.4e-124	79.50	high chlorophyll fluorescent 109	[more]
AT1G56350.1	8.4e-64	38.07	Peptide chain release factor 2	[more]
AT3G57190.1	3.5e-54	34.86	peptide chain release factor, putative	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 97..117
None	No IPR available	GENE3D	1.20.58.410	Release factor	coord: 7..124 e-value: 7.1E-12 score: 47.4
None	No IPR available	GENE3D	3.30.160.20		coord: 227..323 e-value: 5.0E-88 score: 295.9
None	No IPR available	GENE3D	3.30.70.1660		coord: 131..361 e-value: 5.0E-88 score: 295.9
None	No IPR available	PANTHER	PTHR43116	PEPTIDE CHAIN RELEASE FACTOR 2	coord: 1..371
None	No IPR available	PANTHER	PTHR43116:SF3	PEPTIDE CHAIN RELEASE FACTOR PRFB1, CHLOROPLASTIC-RELATED	coord: 1..371
None	No IPR available	SUPERFAMILY	75620	Release factor	coord: 28..369
IPR005139	Peptide chain release factor	SMART	SM00937	PCRF_a_2	coord: 84..196 e-value: 8.8E-43 score: 158.1
IPR005139	Peptide chain release factor	PFAM	PF03462	PCRF	coord: 29..221 e-value: 3.9E-51 score: 173.6
IPR000352	Peptide chain release factor class I	PFAM	PF00472	RF-1	coord: 232..340 e-value: 2.9E-35 score: 120.7
IPR000352	Peptide chain release factor class I	PROSITE	PS00745	RF_PROK_I	coord: 247..263
IPR004374	Peptide chain release factor 2	TIGRFAM	TIGR00020	TIGR00020	coord: 4..367 e-value: 1.1E-123 score: 410.8
IPR004374	Peptide chain release factor 2	HAMAP	MF_00094	Rel_fac_2	coord: 1..368 score: 36.347511

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS013206	MS013206	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS013206.1-cds	MS013206.1-cds-scaffold459:1194192..1194416	CDS
MS013206.1-cds	MS013206.1-cds-scaffold459:1195201..1195404	CDS
MS013206.1-cds	MS013206.1-cds-scaffold459:1195565..1195780	CDS
MS013206.1-cds	MS013206.1-cds-scaffold459:1196021..1196207	CDS
MS013206.1-cds	MS013206.1-cds-scaffold459:1196629..1196930	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS013206.1	MS013206.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006415	translational termination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0003747	translation release factor activity
molecular_function	GO:0016149	translation release factor activity, codon specific