MS012960.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS012960.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Eukaryotic aspartyl protease family protein
Location	scaffold38: 928166 .. 929599 (-)
Sequence length	1434
RNA-Seq Expression	MS012960.1
Synteny	MS012960.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCATGGCTTCCCCTGTTTTCCTCCTCCTCTGTTTTCTCCTCTCTTCCTCTGTTTCCTCATCCCAGATTCTGCTTCTCCCTCTCTCCCATTCCTTGTCAAAATCAGACGTCAACAACAACACCCATAATCTCCTCAAATCCACTGCAGCCCGCTCCGCCGCCAGATTCCACCGCCGCCGCCGCCAAAGCCAGGTCTCTCTCCCGCTCTCTCCCGGCGGCGATTACACCCTCTCCTTCAACCTCGGCTCCTCCCCTCCTCAGCCCATTTCTCTTTACATGGACACCGGCAGCGACCTCGTTTGGTTCCCCTGTTCCCCTTTCGAATGCATTCTCTGCGAAGGAAAGCCCAAAATTCCATCCCCTCTCCCAAAAATTCCCCAAAACAAATCGGTTTCCTGCAGCGCCGCAGCCTGCGCCGCCGCGCACGGCGGCTCCCTCTCGGCCTCCCACCTCTGCGCAATTTCCCGGTGCCCCCTCGAGTCGATTGAAATTTCGGAGTGCGCTGAGTTCTCTTGCCCGCCGTTCTATTACGCTTATGGCGATGGAAGCTTGATTGCTGAGCTTTATAGGGATAGTCTCCGGCTGCCGGCTCCGGCGCCGGCAATTGGTGTCCGGAATTTTACTTTCGGGTGCGCCCACACGGCGCTGGGCGAGCCGATTGGCGTCGCCGGGTTCGGCCGGGGGGCGCTGTCGATGCCGAGTCAACTCGCTACTTTTTCGCCCCAATTGGGCAATCAGTTCTCCTACTGTTTGATTTCTCACTCGTTTGCGGCGGACCGAGTCCGCCGCCCGAGTCCGCTCATTCTCGGCCGGTACGATCGCGGCGAGGCGGCGGCGGAGTTTGTTTACACTTCCATCCTCGAGAATCCGAAACATCCTTATTTCTACTCTGTTGGGCTGGCCGGAATTTCGGTCGGCGCGGCGAGGATTCCGGCGCCGGAGTTCTTGAAGCGGGTGGACGAGAGCGGCGGCGGCGGCGTTGTGGTGGATTCCGGCACTACTTTCACTATGCTGCCGGCGAGTTTGTATGACTCGGTGGTAGTTGAGTTCGAGAACCGAGTCGGGCGAGTTCTGAACCGGGCGAGTCAGATCGAGGAGAATACTGGGCTCAGACCTTGCTATTACTACGAGAACTCTATAGACGTGCCACGTTTCGTGCTGCACTTCGTCGGGGAAAAATCAAGTGTGGTGCTGCCTAGGAAGAATTATTTTTACGAGTTTTTGGACGGTGGCGATGGCGTGGGGATGAAGAGGAGGGTCGGGTGTCTGATGCTGATGAACGGTGGAGATGAGGCAGAGCTGGCCGGTGGGCCCGGGGCCACGCTTGGGAATTATCAACAACAGGGTTTTGAGGTGGTTTATGATTTGGAGAAGAACCGGGTCGGGTTCGCCCGGCGACAGTGTTCTACGCTGTGGGATAGTTTGAACCGGAGC

mRNA sequence

TCCATGGCTTCCCCTGTTTTCCTCCTCCTCTGTTTTCTCCTCTCTTCCTCTGTTTCCTCATCCCAGATTCTGCTTCTCCCTCTCTCCCATTCCTTGTCAAAATCAGACGTCAACAACAACACCCATAATCTCCTCAAATCCACTGCAGCCCGCTCCGCCGCCAGATTCCACCGCCGCCGCCGCCAAAGCCAGGTCTCTCTCCCGCTCTCTCCCGGCGGCGATTACACCCTCTCCTTCAACCTCGGCTCCTCCCCTCCTCAGCCCATTTCTCTTTACATGGACACCGGCAGCGACCTCGTTTGGTTCCCCTGTTCCCCTTTCGAATGCATTCTCTGCGAAGGAAAGCCCAAAATTCCATCCCCTCTCCCAAAAATTCCCCAAAACAAATCGGTTTCCTGCAGCGCCGCAGCCTGCGCCGCCGCGCACGGCGGCTCCCTCTCGGCCTCCCACCTCTGCGCAATTTCCCGGTGCCCCCTCGAGTCGATTGAAATTTCGGAGTGCGCTGAGTTCTCTTGCCCGCCGTTCTATTACGCTTATGGCGATGGAAGCTTGATTGCTGAGCTTTATAGGGATAGTCTCCGGCTGCCGGCTCCGGCGCCGGCAATTGGTGTCCGGAATTTTACTTTCGGGTGCGCCCACACGGCGCTGGGCGAGCCGATTGGCGTCGCCGGGTTCGGCCGGGGGGCGCTGTCGATGCCGAGTCAACTCGCTACTTTTTCGCCCCAATTGGGCAATCAGTTCTCCTACTGTTTGATTTCTCACTCGTTTGCGGCGGACCGAGTCCGCCGCCCGAGTCCGCTCATTCTCGGCCGGTACGATCGCGGCGAGGCGGCGGCGGAGTTTGTTTACACTTCCATCCTCGAGAATCCGAAACATCCTTATTTCTACTCTGTTGGGCTGGCCGGAATTTCGGTCGGCGCGGCGAGGATTCCGGCGCCGGAGTTCTTGAAGCGGGTGGACGAGAGCGGCGGCGGCGGCGTTGTGGTGGATTCCGGCACTACTTTCACTATGCTGCCGGCGAGTTTGTATGACTCGGTGGTAGTTGAGTTCGAGAACCGAGTCGGGCGAGTTCTGAACCGGGCGAGTCAGATCGAGGAGAATACTGGGCTCAGACCTTGCTATTACTACGAGAACTCTATAGACGTGCCACGTTTCGTGCTGCACTTCGTCGGGGAAAAATCAAGTGTGGTGCTGCCTAGGAAGAATTATTTTTACGAGTTTTTGGACGGTGGCGATGGCGTGGGGATGAAGAGGAGGGTCGGGTGTCTGATGCTGATGAACGGTGGAGATGAGGCAGAGCTGGCCGGTGGGCCCGGGGCCACGCTTGGGAATTATCAACAACAGGGTTTTGAGGTGGTTTATGATTTGGAGAAGAACCGGGTCGGGTTCGCCCGGCGACAGTGTTCTACGCTGTGGGATAGTTTGAACCGGAGC

Coding sequence (CDS)

TCCATGGCTTCCCCTGTTTTCCTCCTCCTCTGTTTTCTCCTCTCTTCCTCTGTTTCCTCATCCCAGATTCTGCTTCTCCCTCTCTCCCATTCCTTGTCAAAATCAGACGTCAACAACAACACCCATAATCTCCTCAAATCCACTGCAGCCCGCTCCGCCGCCAGATTCCACCGCCGCCGCCGCCAAAGCCAGGTCTCTCTCCCGCTCTCTCCCGGCGGCGATTACACCCTCTCCTTCAACCTCGGCTCCTCCCCTCCTCAGCCCATTTCTCTTTACATGGACACCGGCAGCGACCTCGTTTGGTTCCCCTGTTCCCCTTTCGAATGCATTCTCTGCGAAGGAAAGCCCAAAATTCCATCCCCTCTCCCAAAAATTCCCCAAAACAAATCGGTTTCCTGCAGCGCCGCAGCCTGCGCCGCCGCGCACGGCGGCTCCCTCTCGGCCTCCCACCTCTGCGCAATTTCCCGGTGCCCCCTCGAGTCGATTGAAATTTCGGAGTGCGCTGAGTTCTCTTGCCCGCCGTTCTATTACGCTTATGGCGATGGAAGCTTGATTGCTGAGCTTTATAGGGATAGTCTCCGGCTGCCGGCTCCGGCGCCGGCAATTGGTGTCCGGAATTTTACTTTCGGGTGCGCCCACACGGCGCTGGGCGAGCCGATTGGCGTCGCCGGGTTCGGCCGGGGGGCGCTGTCGATGCCGAGTCAACTCGCTACTTTTTCGCCCCAATTGGGCAATCAGTTCTCCTACTGTTTGATTTCTCACTCGTTTGCGGCGGACCGAGTCCGCCGCCCGAGTCCGCTCATTCTCGGCCGGTACGATCGCGGCGAGGCGGCGGCGGAGTTTGTTTACACTTCCATCCTCGAGAATCCGAAACATCCTTATTTCTACTCTGTTGGGCTGGCCGGAATTTCGGTCGGCGCGGCGAGGATTCCGGCGCCGGAGTTCTTGAAGCGGGTGGACGAGAGCGGCGGCGGCGGCGTTGTGGTGGATTCCGGCACTACTTTCACTATGCTGCCGGCGAGTTTGTATGACTCGGTGGTAGTTGAGTTCGAGAACCGAGTCGGGCGAGTTCTGAACCGGGCGAGTCAGATCGAGGAGAATACTGGGCTCAGACCTTGCTATTACTACGAGAACTCTATAGACGTGCCACGTTTCGTGCTGCACTTCGTCGGGGAAAAATCAAGTGTGGTGCTGCCTAGGAAGAATTATTTTTACGAGTTTTTGGACGGTGGCGATGGCGTGGGGATGAAGAGGAGGGTCGGGTGTCTGATGCTGATGAACGGTGGAGATGAGGCAGAGCTGGCCGGTGGGCCCGGGGCCACGCTTGGGAATTATCAACAACAGGGTTTTGAGGTGGTTTATGATTTGGAGAAGAACCGGGTCGGGTTCGCCCGGCGACAGTGTTCTACGCTGTGGGATAGTTTGAACCGGAGC

Protein sequence

SMASPVFLLLCFLLSSSVSSSQILLLPLSHSLSKSDVNNNTHNLLKSTAARSAARFHRRRRQSQVSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPKIPSPLPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYYAYGDGSLIAELYRDSLRLPAPAPAIGVRNFTFGCAHTALGEPIGVAGFGRGALSMPSQLATFSPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHPYFYSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENRVGRVLNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGMKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLNRS

Homology

[Close]

BLAST of MS012960.1 vs. NCBI nr
Match: XP_023553227.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 817.4 bits (2110), Expect = 6.5e-233
Identity = 415/485 (85.57%), Postives = 436/485 (89.90%), Query Frame = 0


Query: 2   MASPVFL-LLCFLLSSSVSSSQILLLPLSHSLSKSDVNNNTHNLLKSTAARSAARFHRRR 61
           MASPVFL LLCFLLSS V SSQ+LLLPLS+SLS S   NNTHNLLKSTAARS+ARFH RR
Sbjct: 1   MASPVFLFLLCFLLSSPVFSSQLLLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 62  R---QSQVSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPK 121
           R   +S +SLPLSPGGDYTLSFNLGS   Q ISLYMDTGSDLVWFPCSPFECILCEGKPK
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSE-SQKISLYMDTGSDLVWFPCSPFECILCEGKPK 120

Query: 122 IPSPLPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYY 181
           I SPLPKI   KSVSCSAAAC+AAHGGSLSASHLCAISRCPLESIE+SEC+ FSCPPFYY
Sbjct: 121 IQSPLPKIADKKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYY 180

Query: 182 AYGDGSLIAELYRDSLRLPAPA----PAIGVRNFTFGCAHTALGEPIGVAGFGRGALSMP 241
           AYGDGSLI  LYRDSL LPAPA    PAI VRNFTFGCAH+ALGEPIGVAGFGRG LSMP
Sbjct: 181 AYGDGSLIGRLYRDSLSLPAPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMP 240

Query: 242 SQLATFSPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHP 301
           SQLATFSPQLGN+FSYCL+SHSFAADRVRRPSPLILGRY   E   EF+YTS+LENPKHP
Sbjct: 241 SQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSE--TEFIYTSLLENPKHP 300

Query: 302 YFYSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENR 361
           YFYSVGLAGISVG+ RIPAPEFLKRVDE G GGVVVDSGTTFTMLPA LY+SVV +FENR
Sbjct: 301 YFYSVGLAGISVGSVRIPAPEFLKRVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENR 360

Query: 362 VGRVLNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDG 421
            GRV +RAS+IEENTGL PCYYYENS++VPR VLHFVGEKSSVVLPRKNYFYEFLDGGDG
Sbjct: 361 TGRVASRASRIEENTGLSPCYYYENSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDG 420

Query: 422 VGMKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWD 479
           V  KR+VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWD
Sbjct: 421 VERKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWD 480

[Close]

BLAST of MS012960.1 vs. NCBI nr
Match: KAG6577689.1 (putative aspartyl protease, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 811.2 bits (2094), Expect = 4.7e-231
Identity = 412/485 (84.95%), Postives = 434/485 (89.48%), Query Frame = 0


Query: 2   MASPVFL-LLCFLLSSSVSSSQILLLPLSHSLSKSDVNNNTHNLLKSTAARSAARFHRRR 61
           MASPVFL LLCFL+SS V SSQ+LLLPLS+SLS S   NNTHNLLKSTAARS+ARFH RR
Sbjct: 1   MASPVFLFLLCFLISSPVFSSQLLLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 62  R---QSQVSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPK 121
           R   +S +SLPLSPGGDYTLSFNLGS   Q ISLYMDTGSDLVWFPCSPFECILCEGKPK
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSE-SQKISLYMDTGSDLVWFPCSPFECILCEGKPK 120

Query: 122 IPSPLPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYY 181
           I SPLPKI   KSVSCSAAAC+AAHGGSLSASHLCAISRCPLESIE+SEC+ FSCPPFYY
Sbjct: 121 IQSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYY 180

Query: 182 AYGDGSLIAELYRDSLRLPAPA----PAIGVRNFTFGCAHTALGEPIGVAGFGRGALSMP 241
           AYGDGSLI  LYRDSL LPAPA    PAI VRNFTFGCAH+ALGEPIGVAGFGRG LSMP
Sbjct: 181 AYGDGSLIGRLYRDSLSLPAPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMP 240

Query: 242 SQLATFSPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHP 301
           SQLATFSPQLGN+FSYCL+SHSFAADRVRRPSPLILGRY   E   EF+YTS+LENPKHP
Sbjct: 241 SQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSE--TEFIYTSMLENPKHP 300

Query: 302 YFYSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENR 361
           YFYSVGLAGISVG+ RIPAPEFLKRVDE G GGVVVDSGTTFTMLPA LY+SVV +FENR
Sbjct: 301 YFYSVGLAGISVGSVRIPAPEFLKRVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENR 360

Query: 362 VGRVLNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDG 421
            GRV +RAS+IEENTGL PCY YE S++VPR VLHFVGEKSSV LPRKNYFYEFLDGGDG
Sbjct: 361 TGRVASRASRIEENTGLSPCYSYEKSVEVPRVVLHFVGEKSSVELPRKNYFYEFLDGGDG 420

Query: 422 VGMKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWD 479
           VG KR+VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWD
Sbjct: 421 VGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWD 480

[Close]

BLAST of MS012960.1 vs. NCBI nr
Match: XP_022923540.1 (probable aspartyl protease At4g16563 [Cucurbita moschata])

HSP 1 Score: 810.4 bits (2092), Expect = 8.0e-231
Identity = 412/485 (84.95%), Postives = 433/485 (89.28%), Query Frame = 0


Query: 2   MASPVFL-LLCFLLSSSVSSSQILLLPLSHSLSKSDVNNNTHNLLKSTAARSAARFHRRR 61
           MASPVFL LLCFL SS V SSQ+LLLPLS+SLS S   NNTHNLLKSTAARS+ARFH RR
Sbjct: 1   MASPVFLFLLCFLFSSPVFSSQLLLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 62  R---QSQVSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPK 121
           R   +S +SLPLSPGGDYTLSFNLGS   Q ISLYMDTGSDLVWFPCSPFECILCEGKPK
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSE-SQKISLYMDTGSDLVWFPCSPFECILCEGKPK 120

Query: 122 IPSPLPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYY 181
           I SPLPKI   KSVSCSAAAC+AAHGGSLSASHLCAISRCPLESIE+SEC+ FSCPPFYY
Sbjct: 121 IQSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYY 180

Query: 182 AYGDGSLIAELYRDSLRLPAPA----PAIGVRNFTFGCAHTALGEPIGVAGFGRGALSMP 241
           AYGDGSLI  LYRDSL LPAPA    PAI VRNFTFGCAH+ALGEPIGVAGFGRG LSMP
Sbjct: 181 AYGDGSLIGRLYRDSLSLPAPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMP 240

Query: 242 SQLATFSPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHP 301
           SQLATFSPQLGN+FSYCL+SHSFAADRVRRPSPLILGRY   E   EF+YTS+LENPKHP
Sbjct: 241 SQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSE--TEFIYTSMLENPKHP 300

Query: 302 YFYSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENR 361
           YFYSVGLAGISVG+ RIPAPEFLKRVDE G GGVVVDSGTTFTMLPA LY+SVV +FENR
Sbjct: 301 YFYSVGLAGISVGSVRIPAPEFLKRVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENR 360

Query: 362 VGRVLNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDG 421
            GRV +RAS+IEENTGL PCY YE S++VPR VLHFVGEKSSV LPRKNYFYEFLDGGDG
Sbjct: 361 TGRVASRASRIEENTGLSPCYSYEKSVEVPRVVLHFVGEKSSVELPRKNYFYEFLDGGDG 420

Query: 422 VGMKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWD 479
           VG KR+VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWD
Sbjct: 421 VGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWD 480

[Close]

BLAST of MS012960.1 vs. NCBI nr
Match: XP_023007805.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 808.9 bits (2088), Expect = 2.3e-230
Identity = 411/483 (85.09%), Postives = 432/483 (89.44%), Query Frame = 0


Query: 2   MASPVFL-LLCFLLSSSVSSSQILLLPLSHSLSKSDVNNNTHNLLKSTAARSAARFHRRR 61
           MASPVFL LLCFLL S V SSQILLLPLS+SLS S   NNTHNLLKSTAARS+ARFH RR
Sbjct: 1   MASPVFLFLLCFLLPSPVFSSQILLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 62  R---QSQVSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPK 121
           R   +S +SLPLSPGGDYTLSFNLGS   Q ISLYMDTGSDLVWFPCSPFECILCEGKPK
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSE-SQKISLYMDTGSDLVWFPCSPFECILCEGKPK 120

Query: 122 IPSPLPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYY 181
           I SPLPKI   KSVSCSAAAC+AAHGGSLSASHLCAISRCPLESIE+SEC+ FSCPPFYY
Sbjct: 121 IQSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYY 180

Query: 182 AYGDGSLIAELYRDSLRLPAPA--PAIGVRNFTFGCAHTALGEPIGVAGFGRGALSMPSQ 241
           AYGDGSLI  LYRDSL LPAPA  PAI VRNFTFGCAH+ALGEPIGVAGFGRG LSMP Q
Sbjct: 181 AYGDGSLIGRLYRDSLSLPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPIQ 240

Query: 242 LATFSPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHPYF 301
           LATFSPQLGN+FSYCL+SHSFAADRVRRPSPLILGRY   E   EF+YTS+LENPKHPYF
Sbjct: 241 LATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSE--TEFIYTSMLENPKHPYF 300

Query: 302 YSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENRVG 361
           YSVGLAGISVG+  IPAPEFLK+VDE G GGVVVDSGTTFTMLPA LY+SVV +FENR G
Sbjct: 301 YSVGLAGISVGSVMIPAPEFLKKVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTG 360

Query: 362 RVLNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDGVG 421
           RV +RASQIEENTGL PCYYYE S++VPR VLHFVGEKSSV+LPRKNYFYEFLDGGDGVG
Sbjct: 361 RVASRASQIEENTGLSPCYYYEKSVEVPRVVLHFVGEKSSVMLPRKNYFYEFLDGGDGVG 420

Query: 422 MKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSL 479
            K +VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWDSL
Sbjct: 421 RKIKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSL 480

[Close]

BLAST of MS012960.1 vs. NCBI nr
Match: XP_038905814.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 807.7 bits (2085), Expect = 5.2e-230
Identity = 410/484 (84.71%), Postives = 432/484 (89.26%), Query Frame = 0


Query: 2   MASPVF-LLLCFLLSSSVSSSQILLLPLSHSLSKSDVN-NNTHNLLKSTAARSAARFHRR 61
           MAS VF LLLCFLLSS V SSQ+LLLPLSHSLS S  + NNTHNLLKSTAARS+ARFH R
Sbjct: 1   MASSVFVLLLCFLLSSPVFSSQLLLLPLSHSLSSSISDFNNTHNLLKSTAARSSARFHHR 60

Query: 62  RR---QSQVSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKP 121
           RR    + +SLPLSPGGDYTLSFNLGS     ISLYMDTGSDLVWFPCSPFECILCEGKP
Sbjct: 61  RRTQHHNHLSLPLSPGGDYTLSFNLGSE-SHKISLYMDTGSDLVWFPCSPFECILCEGKP 120

Query: 122 KIPSPLPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFY 181
           K+ SPLPKI  NKSVSCSA AC+AAHGGSLSASHLCAIS+CPLESIEISEC+ FSCPPFY
Sbjct: 121 KVQSPLPKISNNKSVSCSAPACSAAHGGSLSASHLCAISQCPLESIEISECSSFSCPPFY 180

Query: 182 YAYGDGSLIAELYRDSLRLPAPA--PAIGVRNFTFGCAHTALGEPIGVAGFGRGALSMPS 241
           YAYGDGSLIA LYRDSL LPAPA  PAI VRNFTFGCAHTALGEP+GVAGFGRG LSMPS
Sbjct: 181 YAYGDGSLIARLYRDSLSLPAPAPSPAINVRNFTFGCAHTALGEPVGVAGFGRGTLSMPS 240

Query: 242 QLATFSPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHPY 301
           QLATFSPQLGN+FSYCL+SHSFAA+RVRRPSPLILGRY  GE   EF+YTS+LENPKHPY
Sbjct: 241 QLATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGE--TEFIYTSLLENPKHPY 300

Query: 302 FYSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENRV 361
           FYSVGL GISVG   IPAPEFLK+VDE G GGVVVDSGTTFTMLPA LYDSVV  FENR 
Sbjct: 301 FYSVGLTGISVGNMMIPAPEFLKKVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAAFENRT 360

Query: 362 GRVLNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDGV 421
           GRV NRA +IEENTGL PCYYYENS++VPR VLHFVGEKSSV+LP+KNYFYEFLDGGDGV
Sbjct: 361 GRVANRARRIEENTGLSPCYYYENSVEVPRVVLHFVGEKSSVLLPKKNYFYEFLDGGDGV 420

Query: 422 GMKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDS 479
           G KR+VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDL KNRVGFARRQCSTLWDS
Sbjct: 421 GKKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLAKNRVGFARRQCSTLWDS 480

[Close]

BLAST of MS012960.1 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 1.6e-165
Identity = 299/492 (60.77%), Postives = 367/492 (74.59%), Query Frame = 0


Query: 9   LLCFLLSSSVSS-SQILLLPLSHSLSKSDVNNNTHNLLKSTAARSAARF---HRRRRQSQ 68
           +L +    SVSS S  LLL LSHSLS S  +++  +LLKS+++RS+ARF   H +++Q Q
Sbjct: 13  ILQYYFHFSVSSLSTPLLLHLSHSLSTSKHSSSPLHLLKSSSSRSSARFRRHHHKQQQQQ 72

Query: 69  VSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPKIPSPLPK 128
           +SLP+S G DY +S ++GSS    +SLY+DTGSDLVWFPC PF CILCE KP  PSP   
Sbjct: 73  LSLPISSGSDYLISLSVGSS-SSAVSLYLDTGSDLVWFPCRPFTCILCESKPLPPSPPSS 132

Query: 129 IPQN-KSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISEC--AEFSCPPFYYAYGD 188
           +  +  +VSCS+ +C+AAH  SL +S LCAIS CPL+ IE  +C  + + CPPFYYAYGD
Sbjct: 133 LSSSATTVSCSSPSCSAAH-SSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGD 192

Query: 189 GSLIAELYRDSLRLPAPAPAIGVRNFTFGCAHTALGEPIGVAGFGRGALSMPSQLATFSP 248
           GSL+A+LY DSL L    P++ V NFTFGCAHT L EPIGVAGFGRG LS+P+QLA  SP
Sbjct: 193 GSLVAKLYSDSLSL----PSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSP 252

Query: 249 QLGNQFSYCLISHSFAADRVRRPSPLILGRY------------------DRGEAAAEFVY 308
            LGN FSYCL+SHSF +DRVRRPSPLILGR+                  D  +   EFV+
Sbjct: 253 HLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVF 312

Query: 309 TSILENPKHPYFYSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLY 368
           T +LENPKHPYFYSV L GIS+G   IPAP  L+R+D++GGGGVVVDSGTTFTMLPA  Y
Sbjct: 313 TEMLENPKHPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFY 372

Query: 369 DSVVVEFENRVGRVLNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNY 428
           +SVV EF++RVGRV  RA ++E ++G+ PCYY   ++ VP  VLHF G +SSV LPR+NY
Sbjct: 373 NSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLNQTVKVPALVLHFAGNRSSVTLPRRNY 432

Query: 429 FYEFLDGGDGVGMKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGF 476
           FYEF+DGGDG   KR++GCLMLMNGGDE+EL GG GA LGNYQQQGFEVVYDL   RVGF
Sbjct: 433 FYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGF 492

[Close]

BLAST of MS012960.1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 7.2e-33
Identity = 130/447 (29.08%), Postives = 190/447 (42.51%), Query Frame = 0


Query: 35  SDVNNNTHNLLKSTAARSAARFHRRRRQ----SQVSLPLSPG-GDYTLSFNLGSSPPQPI 94
           S  N     LL+    R + R  R        S V   +  G G+Y ++ ++G +P QP 
Sbjct: 50  SGKNLTKFQLLERAIERGSRRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIG-TPAQPF 109

Query: 95  SLYMDTGSDLVWFPCSPFECILCEGKPKIPSPLPKIPQNKSVSCSAAACAAAHGGSLSAS 154
           S  MDTGSDL+W  C P  C  C          P      S S S   C         +S
Sbjct: 110 SAIMDTGSDLIWTQCQP--CTQC-----FNQSTPIFNPQGSSSFSTLPC---------SS 169

Query: 155 HLCAISRCPLESIEISECAEFSCPPFYYAYGDGSLIAELYRDSLRLPAPAPAIGVRNFTF 214
            LC       +++    C+   C  + Y YGDGS   E             ++ + N TF
Sbjct: 170 QLC-------QALSSPTCSNNFC-QYTYGYGDGS---ETQGSMGTETLTFGSVSIPNITF 229

Query: 215 GCAHT----ALGEPIGVAGFGRGALSMPSQLATFSPQLGNQFSYCLISHSFAADRVRRPS 274
           GC         G   G+ G GRG LS+PSQL         +FSYC+     +      PS
Sbjct: 230 GCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDV------TKFSYCMTPIGSST-----PS 289

Query: 275 PLILGRYDRGEAAAEFVYTSILENPKHPYFYSVGLAGISVGAARIPA-PEFLKRVDESGG 334
            L+LG       A     T+++++ + P FY + L G+SVG+ R+P  P        +G 
Sbjct: 290 NLLLGSLANSVTAGS-PNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGT 349

Query: 335 GGVVVDSGTTFTMLPASLYDSVVVEFENRVGRVLNRASQIEENTGLRPCYYY---ENSID 394
           GG+++DSGTT T    + Y SV  EF +++   +   S    ++G   C+      +++ 
Sbjct: 350 GGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGS----SSGFDLCFQTPSDPSNLQ 409

Query: 395 VPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGMKRRVGCLMLMNGGDEAELAGGPGAT 454
           +P FV+HF G    + LP +NYF    +G         + CL + +      +       
Sbjct: 410 IPTFVMHFDG--GDLELPSENYFISPSNG---------LICLAMGSSSQGMSI------- 434

Query: 455 LGNYQQQGFEVVYDLEKNRVGFARRQC 469
            GN QQQ   VVYD   + V FA  QC
Sbjct: 470 FGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of MS012960.1 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 9.4e-33
Identity = 119/416 (28.61%), Postives = 180/416 (43.27%), Query Frame = 0


Query: 66  SLPLSPG-----GDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPKIPS 125
           S+P++ G     G+Y +   LG +PPQ + + +DT +D VW PCS      C G     +
Sbjct: 90  SVPVASGNQLHIGNYVVRAKLG-TPPQLMFMVLDTSNDAVWLPCSG-----CSGCSNAST 149

Query: 126 PLPKIPQN--KSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYYA 185
                  +   +VSCS A C  A G             CP  S + S C+      F  +
Sbjct: 150 SFNTNSSSTYSTVSCSTAQCTQARG-----------LTCPSSSPQPSVCS------FNQS 209

Query: 186 Y-GDGSLIAELYRDSLRLPAPAPAIGVRNFTFGCAHTALGE---PIGVAGFGRGALSMPS 245
           Y GD S  A L +D+L L   AP + + NF+FGC ++A G    P G+ G GRG +S+ S
Sbjct: 210 YGGDSSFSASLVQDTLTL---APDV-IPNFSFGCINSASGNSLPPQGLMGLGRGPMSLVS 269

Query: 246 QLATFSPQLGNQFSYCLIS-HSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHP 305
           Q  +        FSYCL S  SF          L LG   + ++     YT +L NP+ P
Sbjct: 270 QTTSL---YSGVFSYCLPSFRSFYFS-----GSLKLGLLGQPKSIR---YTPLLRNPRRP 329

Query: 306 YFYSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENR 365
             Y V L G+SVG+ ++P        D + G G ++DSGT  T     +Y+++  EF  +
Sbjct: 330 SLYYVNLTGVSVGSVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQ 389

Query: 366 VGRVLNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDG 425
           V       S          C+  +N    P+  LH       + LP +N           
Sbjct: 390 V-----NVSSFSTLGAFDTCFSADNENVAPKITLHMT--SLDLKLPMENTL--------- 449

Query: 426 VGMKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCS 470
             +    G L  ++     + A      + N QQQ   +++D+  +R+G A   C+
Sbjct: 450 --IHSSAGTLTCLSMAGIRQNANAVLNVIANLQQQNLRILFDVPNSRIGIAPEPCN 449

[Close]

BLAST of MS012960.1 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 1.2e-32
Identity = 133/418 (31.82%), Postives = 187/418 (44.74%), Query Frame = 0


Query: 63  SQVSLPLSPG-GDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPKIPSP 122
           S V   LS G G+Y     +G +P + + + +DTGSD+VW  C+P  C  C  +      
Sbjct: 129 SSVVSGLSQGSGEYFTRLGVG-TPARYVYMVLDTGSDIVWLQCAP--CRRCYSQSD---- 188

Query: 123 LPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYYAYGD 182
            P     KS + +   C++ H   L ++  C   R          C       +  +YGD
Sbjct: 189 -PIFDPRKSKTYATIPCSSPHCRRLDSAG-CNTRR--------KTCL------YQVSYGD 248

Query: 183 GSL-IAELYRDSLRLPAPAPAIGVRNFTFGCAHTALGEPIGVA---GFGRGALSMPSQLA 242
           GS  + +   ++L          V+    GC H   G  +G A   G G+G LS P Q  
Sbjct: 249 GSFTVGDFSTETLTFRRNR----VKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQT- 308

Query: 243 TFSPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHPYFYS 302
               +   +FSYCL+  S ++    +PS ++ G       A    +T +L NPK   FY 
Sbjct: 309 --GHRFNQKFSYCLVDRSASS----KPSSVVFGNAAVSRIAR---FTPLLSNPKLDTFYY 368

Query: 303 VGLAGISVGAARIP-APEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENRVG- 362
           VGL GISVG  R+P     L ++D+ G GGV++DSGT+ T L    Y ++   F  RVG 
Sbjct: 369 VGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAF--RVGA 428

Query: 363 RVLNRASQIEENTGLRPCYYYE--NSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDG 422
           + L RA      +    C+     N + VP  VLHF G  + V LP  NY          
Sbjct: 429 KTLKRAPDF---SLFDTCFDLSNMNEVKVPTVVLHFRG--ADVSLPATNYLIP------- 485

Query: 423 VGMKRRVGCLMLMNGGDEAELAGGPG--ATLGNYQQQGFEVVYDLEKNRVGFARRQCS 470
                     +  NG      AG  G  + +GN QQQGF VVYDL  +RVGFA   C+
Sbjct: 489 ----------VDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of MS012960.1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.6e-30
Identity = 128/450 (28.44%), Postives = 189/450 (42.00%), Query Frame = 0


Query: 35  SDVNNNTHNLLKSTAARSAARFHRRRRQSQVSLPLSPG---------GDYTLSFNLGSSP 94
           S  N   + L+K    R+  R  RR R     L  S G         G+Y ++  +G +P
Sbjct: 51  SGKNLTKYELIK----RAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIG-TP 110

Query: 95  PQPISLYMDTGSDLVWFPCSPFECILCEGKPKIPSPLPKIPQNKSVSCSAAACAAAHGGS 154
               S  MDTGSDL+W  C P  C  C        P P      S S S   C + +   
Sbjct: 111 DSSFSAIMDTGSDLIWTQCEP--CTQC-----FSQPTPIFNPQDSSSFSTLPCESQYCQD 170

Query: 155 LSASHLCAISRCPLESIEISECAEFSCPPFYYAYGDGSLIAELYRDSLRLPAPAPAIGVR 214
           L           P E+   +EC       + Y YGDGS   + Y  +        +  V 
Sbjct: 171 L-----------PSETCNNNEC------QYTYGYGDGS-TTQGYMATETFTFETSS--VP 230

Query: 215 NFTFGCAHT----ALGEPIGVAGFGRGALSMPSQLATFSPQLGNQFSYCLISHSFAADRV 274
           N  FGC         G   G+ G G G LS+PSQL         QFSYC+ S+  ++   
Sbjct: 231 NIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGV------GQFSYCMTSYGSSS--- 290

Query: 275 RRPSPLILGRYDRGEAAAEFVYTSILENPKHPYFYSVGLAGISVGAARIPAPEFLKRVDE 334
             PS L LG    G        T+++ +  +P +Y + L GI+VG   +  P    ++ +
Sbjct: 291 --PSTLALGSAASGVPEGS-PSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQD 350

Query: 335 SGGGGVVVDSGTTFTMLPASLYDSVVVEFENRVGRVLNRASQIEENTGLRPCYYYE---N 394
            G GG+++DSGTT T LP   Y++V   F +++    N  +  E ++GL  C+      +
Sbjct: 351 DGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQI----NLPTVDESSSGLSTCFQQPSDGS 410

Query: 395 SIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGMKRRVGCLMLMNGGDEAELAGGP 454
           ++ VP   + F G    + L  +N      +G         V CL +   G  ++L    
Sbjct: 411 TVQVPEISMQFDG--GVLNLGEQNILISPAEG---------VICLAM---GSSSQLG--- 435

Query: 455 GATLGNYQQQGFEVVYDLEKNRVGFARRQC 469
            +  GN QQQ  +V+YDL+   V F   QC
Sbjct: 471 ISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of MS012960.1 vs. ExPASy TrEMBL
Match: A0A6J1EC44 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111431201 PE=3 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 3.9e-231
Identity = 412/485 (84.95%), Postives = 433/485 (89.28%), Query Frame = 0


Query: 2   MASPVFL-LLCFLLSSSVSSSQILLLPLSHSLSKSDVNNNTHNLLKSTAARSAARFHRRR 61
           MASPVFL LLCFL SS V SSQ+LLLPLS+SLS S   NNTHNLLKSTAARS+ARFH RR
Sbjct: 1   MASPVFLFLLCFLFSSPVFSSQLLLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 62  R---QSQVSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPK 121
           R   +S +SLPLSPGGDYTLSFNLGS   Q ISLYMDTGSDLVWFPCSPFECILCEGKPK
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSE-SQKISLYMDTGSDLVWFPCSPFECILCEGKPK 120

Query: 122 IPSPLPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYY 181
           I SPLPKI   KSVSCSAAAC+AAHGGSLSASHLCAISRCPLESIE+SEC+ FSCPPFYY
Sbjct: 121 IQSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYY 180

Query: 182 AYGDGSLIAELYRDSLRLPAPA----PAIGVRNFTFGCAHTALGEPIGVAGFGRGALSMP 241
           AYGDGSLI  LYRDSL LPAPA    PAI VRNFTFGCAH+ALGEPIGVAGFGRG LSMP
Sbjct: 181 AYGDGSLIGRLYRDSLSLPAPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMP 240

Query: 242 SQLATFSPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHP 301
           SQLATFSPQLGN+FSYCL+SHSFAADRVRRPSPLILGRY   E   EF+YTS+LENPKHP
Sbjct: 241 SQLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSE--TEFIYTSMLENPKHP 300

Query: 302 YFYSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENR 361
           YFYSVGLAGISVG+ RIPAPEFLKRVDE G GGVVVDSGTTFTMLPA LY+SVV +FENR
Sbjct: 301 YFYSVGLAGISVGSVRIPAPEFLKRVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENR 360

Query: 362 VGRVLNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDG 421
            GRV +RAS+IEENTGL PCY YE S++VPR VLHFVGEKSSV LPRKNYFYEFLDGGDG
Sbjct: 361 TGRVASRASRIEENTGLSPCYSYEKSVEVPRVVLHFVGEKSSVELPRKNYFYEFLDGGDG 420

Query: 422 VGMKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWD 479
           VG KR+VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWD
Sbjct: 421 VGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWD 480

[Close]

BLAST of MS012960.1 vs. ExPASy TrEMBL
Match: A0A6J1L3Z9 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111500303 PE=3 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 1.1e-230
Identity = 411/483 (85.09%), Postives = 432/483 (89.44%), Query Frame = 0


Query: 2   MASPVFL-LLCFLLSSSVSSSQILLLPLSHSLSKSDVNNNTHNLLKSTAARSAARFHRRR 61
           MASPVFL LLCFLL S V SSQILLLPLS+SLS S   NNTHNLLKSTAARS+ARFH RR
Sbjct: 1   MASPVFLFLLCFLLPSPVFSSQILLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 62  R---QSQVSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPK 121
           R   +S +SLPLSPGGDYTLSFNLGS   Q ISLYMDTGSDLVWFPCSPFECILCEGKPK
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSE-SQKISLYMDTGSDLVWFPCSPFECILCEGKPK 120

Query: 122 IPSPLPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYY 181
           I SPLPKI   KSVSCSAAAC+AAHGGSLSASHLCAISRCPLESIE+SEC+ FSCPPFYY
Sbjct: 121 IQSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYY 180

Query: 182 AYGDGSLIAELYRDSLRLPAPA--PAIGVRNFTFGCAHTALGEPIGVAGFGRGALSMPSQ 241
           AYGDGSLI  LYRDSL LPAPA  PAI VRNFTFGCAH+ALGEPIGVAGFGRG LSMP Q
Sbjct: 181 AYGDGSLIGRLYRDSLSLPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPIQ 240

Query: 242 LATFSPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHPYF 301
           LATFSPQLGN+FSYCL+SHSFAADRVRRPSPLILGRY   E   EF+YTS+LENPKHPYF
Sbjct: 241 LATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSE--TEFIYTSMLENPKHPYF 300

Query: 302 YSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENRVG 361
           YSVGLAGISVG+  IPAPEFLK+VDE G GGVVVDSGTTFTMLPA LY+SVV +FENR G
Sbjct: 301 YSVGLAGISVGSVMIPAPEFLKKVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTG 360

Query: 362 RVLNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDGVG 421
           RV +RASQIEENTGL PCYYYE S++VPR VLHFVGEKSSV+LPRKNYFYEFLDGGDGVG
Sbjct: 361 RVASRASQIEENTGLSPCYYYEKSVEVPRVVLHFVGEKSSVMLPRKNYFYEFLDGGDGVG 420

Query: 422 MKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSL 479
            K +VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWDSL
Sbjct: 421 RKIKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSL 480

[Close]

BLAST of MS012960.1 vs. ExPASy TrEMBL
Match: A0A1S3BK28 (aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103490888 PE=3 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 1.6e-229
Identity = 408/482 (84.65%), Postives = 432/482 (89.63%), Query Frame = 0


Query: 4   SPVFL-LLCFLLSSSVSSSQILLLPLSHSLSKSDVN-NNTHNLLKSTAARSAARFHRRRR 63
           SPVF+ LLCFLLSS V SSQI LLPLSHSLS S  + N+THNLLKSTA RS+ARFH R R
Sbjct: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFH-RHR 63

Query: 64  QSQVSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPKIPSP 123
            + +SLPLSPGGDYTLSFNLGS     ISLYMDTGSDLVWFPCSPFECILCEGKPKI SP
Sbjct: 64  HNHLSLPLSPGGDYTLSFNLGSE-SHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSP 123

Query: 124 LPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYYAYGD 183
           LPKI  NKSVSCSAAAC+AAHGGSLSASHLCAISRCPLESIEISEC+ FSCPPFYYAYGD
Sbjct: 124 LPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGD 183

Query: 184 GSLIAELYRDSLRLPAPAPA--IGVRNFTFGCAHTALGEPIGVAGFGRGALSMPSQLATF 243
           GSL+A LYRDSL LP PAP+  I VRNFTFGCAHT LGEP+GVAGFGRG LSMPSQLATF
Sbjct: 184 GSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATF 243

Query: 244 SPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHPYFYSVG 303
           SPQLGN+FSYCL+SHSFAADRVRRPSPLILGRY  GE   EF+YTS+LENPKHPYFYSVG
Sbjct: 244 SPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGE--TEFIYTSLLENPKHPYFYSVG 303

Query: 304 LAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENRVGRVLN 363
           LAGISVG  RIPAPEFL++VDESG GGVVVDSGTTFTMLP+ LY+SVV EFENR G+V N
Sbjct: 304 LAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVAN 363

Query: 364 RASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDG---VGM 423
           RA +IEENTGL PCYYYENS+ VPR VLHFVGEKSSVVLPRKNYFYEFLDGGDG   VG 
Sbjct: 364 RARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGR 423

Query: 424 KRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLN 479
           KR+VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWD+LN
Sbjct: 424 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLN 481

[Close]

BLAST of MS012960.1 vs. ExPASy TrEMBL
Match: A0A5D3CP11 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00280 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.8e-229
Identity = 408/484 (84.30%), Postives = 432/484 (89.26%), Query Frame = 0


Query: 4   SPVFL-LLCFLLSSSVSSSQILLLPLSHSLSKSDVN-NNTHNLLKSTAARSAARFHRRRR 63
           SPVF+ LLCFLLSS V SSQI LLPLSHSLS S  + N+THNLLKSTA RS+ARFH R R
Sbjct: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFH-RHR 63

Query: 64  QSQVSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPKIPSP 123
            + +SLPLSPGGDYTLSFNLGS     ISLYMDTGSDLVWFPCSPFECILCEGKPKI SP
Sbjct: 64  HNHLSLPLSPGGDYTLSFNLGSE-SHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSP 123

Query: 124 LPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYYAYGD 183
           LPKI  NKSVSCSAAAC+AAHGGSLSASHLCAISRCPLESIEISEC+ FSCPPFYYAYGD
Sbjct: 124 LPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGD 183

Query: 184 GSLIAELYRDSLRLPAPAPA----IGVRNFTFGCAHTALGEPIGVAGFGRGALSMPSQLA 243
           GSL+A LYRDSL LP PAPA    I VRNFTFGCAHT LGEP+GVAGFGRG LSMPSQLA
Sbjct: 184 GSLVARLYRDSLSLPTPAPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLA 243

Query: 244 TFSPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHPYFYS 303
           TFSPQLGN+FSYCL+SHSFAADRVRRPSPLILGRY  GE   EF+YTS+LENPKHPYFYS
Sbjct: 244 TFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGE--TEFIYTSLLENPKHPYFYS 303

Query: 304 VGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENRVGRV 363
           VGLAGISVG  RIPAPEFL++VDESG GGVVVDSGTTFTMLP+ LY+SVV EFENR G+V
Sbjct: 304 VGLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKV 363

Query: 364 LNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDG---V 423
            NRA +IEENTGL PCYYY+NS+ VPR VLHFVGEKSSVVLPRKNYFYEFLDGGDG   V
Sbjct: 364 ANRARRIEENTGLSPCYYYQNSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEV 423

Query: 424 GMKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDS 479
           G KR+VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWD+
Sbjct: 424 GRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDN 483

[Close]

BLAST of MS012960.1 vs. ExPASy TrEMBL
Match: A0A0A0L5I7 (Pepsin A OS=Cucumis sativus OX=3659 GN=Csa_3G020060 PE=3 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.6e-229
Identity = 408/480 (85.00%), Postives = 430/480 (89.58%), Query Frame = 0


Query: 4   SPVFL-LLCFLLSSSVSSSQILLLPLSHSLSKSDVN-NNTHNLLKSTAARSAARFHRRRR 63
           SPVF+ LLCFLLSS V SSQI LLPLSHSLS S  + NNTHNLLKSTA RS+ARFH R R
Sbjct: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFH-RHR 63

Query: 64  QSQVSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPKIPSP 123
            + +SLPLSPGGDYTLSFNLGS     ISLYMDTGSDLVWFPCSPFECILCEGKPKI SP
Sbjct: 64  HNHLSLPLSPGGDYTLSFNLGSE-SHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSP 123

Query: 124 LPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYYAYGD 183
           LPKI  NKSVSCSAAAC+AAHGGSLSASHLCAISRCPLESIEISEC+ FSCPPFYYAYGD
Sbjct: 124 LPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAYGD 183

Query: 184 GSLIAELYRDSLRLPAPAPA--IGVRNFTFGCAHTALGEPIGVAGFGRGALSMPSQLATF 243
           GSL+A LYRDSL LP PAP+  I VRNFTFGCAHT LGEP+GVAGFGRG LSMPSQLATF
Sbjct: 184 GSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLATF 243

Query: 244 SPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHPYFYSVG 303
           SPQLGN+FSYCL+SHSFAADRVRRPSPLILGRY  GE   EF+YTS+LENPKHPYFYSVG
Sbjct: 244 SPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGE--TEFIYTSLLENPKHPYFYSVG 303

Query: 304 LAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENRVGRVLN 363
           LAGISVG  RIPAPEFL +VDE G GGVVVDSGTTFTMLPA LY+SVV EFENR G+V N
Sbjct: 304 LAGISVGNIRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVAN 363

Query: 364 RASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDG-VGMKR 423
           RA +IEENTGL PCYYYENS+ VPR VLHFVGEKS+VVLPRKNYFYEFLDGGDG VG KR
Sbjct: 364 RARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKR 423

Query: 424 RVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDSLNRS 479
           +VGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWD+LNRS
Sbjct: 424 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRS 479

[Close]

BLAST of MS012960.1 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 583.9 bits (1504), Expect = 1.1e-166
Identity = 299/492 (60.77%), Postives = 367/492 (74.59%), Query Frame = 0


Query: 9   LLCFLLSSSVSS-SQILLLPLSHSLSKSDVNNNTHNLLKSTAARSAARF---HRRRRQSQ 68
           +L +    SVSS S  LLL LSHSLS S  +++  +LLKS+++RS+ARF   H +++Q Q
Sbjct: 13  ILQYYFHFSVSSLSTPLLLHLSHSLSTSKHSSSPLHLLKSSSSRSSARFRRHHHKQQQQQ 72

Query: 69  VSLPLSPGGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPKIPSPLPK 128
           +SLP+S G DY +S ++GSS    +SLY+DTGSDLVWFPC PF CILCE KP  PSP   
Sbjct: 73  LSLPISSGSDYLISLSVGSS-SSAVSLYLDTGSDLVWFPCRPFTCILCESKPLPPSPPSS 132

Query: 129 IPQN-KSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISEC--AEFSCPPFYYAYGD 188
           +  +  +VSCS+ +C+AAH  SL +S LCAIS CPL+ IE  +C  + + CPPFYYAYGD
Sbjct: 133 LSSSATTVSCSSPSCSAAH-SSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGD 192

Query: 189 GSLIAELYRDSLRLPAPAPAIGVRNFTFGCAHTALGEPIGVAGFGRGALSMPSQLATFSP 248
           GSL+A+LY DSL L    P++ V NFTFGCAHT L EPIGVAGFGRG LS+P+QLA  SP
Sbjct: 193 GSLVAKLYSDSLSL----PSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSP 252

Query: 249 QLGNQFSYCLISHSFAADRVRRPSPLILGRY------------------DRGEAAAEFVY 308
            LGN FSYCL+SHSF +DRVRRPSPLILGR+                  D  +   EFV+
Sbjct: 253 HLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVF 312

Query: 309 TSILENPKHPYFYSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLY 368
           T +LENPKHPYFYSV L GIS+G   IPAP  L+R+D++GGGGVVVDSGTTFTMLPA  Y
Sbjct: 313 TEMLENPKHPYFYSVSLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFY 372

Query: 369 DSVVVEFENRVGRVLNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNY 428
           +SVV EF++RVGRV  RA ++E ++G+ PCYY   ++ VP  VLHF G +SSV LPR+NY
Sbjct: 373 NSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLNQTVKVPALVLHFAGNRSSVTLPRRNY 432

Query: 429 FYEFLDGGDGVGMKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGF 476
           FYEF+DGGDG   KR++GCLMLMNGGDE+EL GG GA LGNYQQQGFEVVYDL   RVGF
Sbjct: 433 FYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGF 492

[Close]

BLAST of MS012960.1 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 206.5 bits (524), Expect = 4.9e-53
Identity = 167/504 (33.13%), Postives = 240/504 (47.62%), Query Frame = 0


Query: 6   VFLLLCFLLSS------------SVSSSQILLLPLSHSLSKSDVNNNTHNLLKSTAARSA 65
           +FLL+  LL++            S SSS  L+L    +L+KS V+  T         +S 
Sbjct: 10  LFLLITLLLNTTNKTQARQHKNPSSSSSSFLVL----TLTKSSVSLPT--------PKSQ 69

Query: 66  ARFHRRRRQSQVSLPLSP----GGDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCS--PF 125
            +   ++  S V + + P       Y ++ N+G +PPQ + +Y+DTGSDL W PC    F
Sbjct: 70  TQERIKKPLSSVDVVMEPLREVRDGYLITLNIG-TPPQAVQVYLDTGSDLTWVPCGNLSF 129

Query: 126 ECILCEG-------KPKIPSPLPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLE 185
           +CI C          P + SPL      +  SC+++ C   H  S +    CA++ C + 
Sbjct: 130 DCIECYDLKNNDLKSPSVFSPLHSSTSFRD-SCASSFCVEIH-SSDNPFDPCAVAGCSVS 189

Query: 186 SIEISECAEFSCPPFYYAYGDGSLIAE-LYRDSLRLPAPAPAIGVRNFTFGCAHTALGEP 245
            +  S C    CP F Y YG+G LI+  L RD L+    A    V  F+FGC  +   EP
Sbjct: 190 MLLKSTCVR-PCPSFAYTYGEGGLISGILTRDILK----ARTRDVPRFSFGCVTSTYREP 249

Query: 246 IGVAGFGRGALSMPSQLATFSPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRG-EAA 305
           IG+AGFGRG LS+PSQL      L   FS+C +   F  +     SPLILG         
Sbjct: 250 IGIAGFGRGLLSLPSQLGF----LEKGFSHCFLPFKF-VNNPNISSPLILGASALSINLT 309

Query: 306 AEFVYTSILENPKHPYFYSVGLAGISVGAARIP--APEFLKRVDESGGGGVVVDSGTTFT 365
               +T +L  P +P  Y +GL  I++G    P   P  L++ D  G GG++VDSGTT+T
Sbjct: 310 DSLQFTPMLNTPMYPNSYYIGLESITIGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYT 369

Query: 366 MLPASLYDSVVVEFENRVGRVLNRASQIEENTGLRPCY----------YYENSIDV--PR 425
            LP   Y  ++   ++ +     RA++ E  TG   CY            EN + +  P 
Sbjct: 370 HLPEPFYSQLLTTLQSTI--TYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPS 429

Query: 426 FVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGMKRRVGCLMLMNGGDEAELAGGPGATLGN 469
              HF+   ++++LP+ N FY      DG      V CL+  N  D      GP    G+
Sbjct: 430 ITFHFL-NNATLLLPQGNSFYAMSAPSDG----SVVQCLLFQNMEDGDY---GPAGVFGS 478

[Close]

BLAST of MS012960.1 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 177.2 bits (448), Expect = 3.2e-44
Identity = 153/500 (30.60%), Postives = 227/500 (45.40%), Query Frame = 0


Query: 2   MASPVFLLLCFLLSSSVSSSQILLLPLSHS-LSKSDVNNNTHNLLKSTAARSAARFH--- 61
           MAS +F      L S VS+ ++ L P SHS  S  D   +   L +S+ AR+    H   
Sbjct: 1   MASSIFFFFLIFL-SVVSAVKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKHGTS 60

Query: 62  -----------RRRRQSQVSLPLSPG--GDYTLSFNLGSSPPQPISLYMDTGSDLVWFPC 121
                           + V  PLS    G Y++S + G +P Q I    DTGS LVW PC
Sbjct: 61  IKPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFG-TPSQTIPFVFDTGSSLVWLPC 120

Query: 122 -SPFECILCEGKPKIPSPLPK-IPQNKS----VSCSAAACAAAHGGSLSASHLCAISRCP 181
            S + C  C+     P+ +P+ IP+N S    + C +  C   +G ++         +C 
Sbjct: 121 TSRYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV---------QCR 180

Query: 182 LESIEISECAEFSCPPFYYAYGDGSLIAELYRDSLRLPAPAPAIGVRNFTFGCAHTALGE 241
                   C    CPP+   YG GS    L  + L      P + V +F  GC+  +  +
Sbjct: 181 GCDPNTRNCT-VGCPPYILQYGLGSTAGVLITEKLDF----PDLTVPDFVVGCSIISTRQ 240

Query: 242 PIGVAGFGRGALSMPSQLATFSPQLGNQFSYCLISHSFAADRVRRPSPLILGR-YDRGEA 301
           P G+AGFGRG +S+PSQ+         +FS+CL+S  F    V     L  G  ++ G  
Sbjct: 241 PAGIAGFGRGPVSLPSQMNL------KRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSK 300

Query: 302 AAEFVYTSILENPK-----HPYFYSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSG 361
                YT   +NP         +Y + L  I VG   +  P        +G GG +VDSG
Sbjct: 301 TPGLTYTPFRKNPNVSNKAFLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSG 360

Query: 362 TTFTMLPASLYDSVVVEFENRVGRVLNRASQIEENTGLRPCYYY--ENSIDVPRFVLHFV 421
           +TFT +   +++ V  EF +++     R   +E+ TGL PC+    +  + VP  +  F 
Sbjct: 361 STFTFMERPVFELVAEEFASQMSN-YTREKDLEKETGLGPCFNISGKGDVTVPELIFEFK 420

Query: 422 GEKSSVVLPRKNYFYEFLDGGDGVGMKRRVGCLMLMNGGDEAELAG-GPGATLGNYQQQG 470
           G  + + LP  NYF  F+   D V       CL +++        G GP   LG++QQQ 
Sbjct: 421 G-GAKLELPLSNYF-TFVGNTDTV-------CLTVVSDKTVNPSGGTGPAIILGSFQQQN 468

[Close]

BLAST of MS012960.1 vs. TAIR 10
Match: AT1G09750.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 142.9 bits (359), Expect = 6.7e-34
Identity = 119/416 (28.61%), Postives = 180/416 (43.27%), Query Frame = 0


Query: 66  SLPLSPG-----GDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPKIPS 125
           S+P++ G     G+Y +   LG +PPQ + + +DT +D VW PCS      C G     +
Sbjct: 90  SVPVASGNQLHIGNYVVRAKLG-TPPQLMFMVLDTSNDAVWLPCSG-----CSGCSNAST 149

Query: 126 PLPKIPQN--KSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYYA 185
                  +   +VSCS A C  A G             CP  S + S C+      F  +
Sbjct: 150 SFNTNSSSTYSTVSCSTAQCTQARG-----------LTCPSSSPQPSVCS------FNQS 209

Query: 186 Y-GDGSLIAELYRDSLRLPAPAPAIGVRNFTFGCAHTALGE---PIGVAGFGRGALSMPS 245
           Y GD S  A L +D+L L   AP + + NF+FGC ++A G    P G+ G GRG +S+ S
Sbjct: 210 YGGDSSFSASLVQDTLTL---APDV-IPNFSFGCINSASGNSLPPQGLMGLGRGPMSLVS 269

Query: 246 QLATFSPQLGNQFSYCLIS-HSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHP 305
           Q  +        FSYCL S  SF          L LG   + ++     YT +L NP+ P
Sbjct: 270 QTTSL---YSGVFSYCLPSFRSFYFS-----GSLKLGLLGQPKSIR---YTPLLRNPRRP 329

Query: 306 YFYSVGLAGISVGAARIPAPEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENR 365
             Y V L G+SVG+ ++P        D + G G ++DSGT  T     +Y+++  EF  +
Sbjct: 330 SLYYVNLTGVSVGSVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQ 389

Query: 366 VGRVLNRASQIEENTGLRPCYYYENSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDG 425
           V       S          C+  +N    P+  LH       + LP +N           
Sbjct: 390 V-----NVSSFSTLGAFDTCFSADNENVAPKITLHMT--SLDLKLPMENTL--------- 449

Query: 426 VGMKRRVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCS 470
             +    G L  ++     + A      + N QQQ   +++D+  +R+G A   C+
Sbjct: 450 --IHSSAGTLTCLSMAGIRQNANAVLNVIANLQQQNLRILFDVPNSRIGIAPEPCN 449

[Close]

BLAST of MS012960.1 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 142.5 bits (358), Expect = 8.7e-34
Identity = 133/418 (31.82%), Postives = 187/418 (44.74%), Query Frame = 0


Query: 63  SQVSLPLSPG-GDYTLSFNLGSSPPQPISLYMDTGSDLVWFPCSPFECILCEGKPKIPSP 122
           S V   LS G G+Y     +G +P + + + +DTGSD+VW  C+P  C  C  +      
Sbjct: 129 SSVVSGLSQGSGEYFTRLGVG-TPARYVYMVLDTGSDIVWLQCAP--CRRCYSQSD---- 188

Query: 123 LPKIPQNKSVSCSAAACAAAHGGSLSASHLCAISRCPLESIEISECAEFSCPPFYYAYGD 182
            P     KS + +   C++ H   L ++  C   R          C       +  +YGD
Sbjct: 189 -PIFDPRKSKTYATIPCSSPHCRRLDSAG-CNTRR--------KTCL------YQVSYGD 248

Query: 183 GSL-IAELYRDSLRLPAPAPAIGVRNFTFGCAHTALGEPIGVA---GFGRGALSMPSQLA 242
           GS  + +   ++L          V+    GC H   G  +G A   G G+G LS P Q  
Sbjct: 249 GSFTVGDFSTETLTFRRNR----VKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQT- 308

Query: 243 TFSPQLGNQFSYCLISHSFAADRVRRPSPLILGRYDRGEAAAEFVYTSILENPKHPYFYS 302
               +   +FSYCL+  S ++    +PS ++ G       A    +T +L NPK   FY 
Sbjct: 309 --GHRFNQKFSYCLVDRSASS----KPSSVVFGNAAVSRIAR---FTPLLSNPKLDTFYY 368

Query: 303 VGLAGISVGAARIP-APEFLKRVDESGGGGVVVDSGTTFTMLPASLYDSVVVEFENRVG- 362
           VGL GISVG  R+P     L ++D+ G GGV++DSGT+ T L    Y ++   F  RVG 
Sbjct: 369 VGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAF--RVGA 428

Query: 363 RVLNRASQIEENTGLRPCYYYE--NSIDVPRFVLHFVGEKSSVVLPRKNYFYEFLDGGDG 422
           + L RA      +    C+     N + VP  VLHF G  + V LP  NY          
Sbjct: 429 KTLKRAPDF---SLFDTCFDLSNMNEVKVPTVVLHFRG--ADVSLPATNYLIP------- 485

Query: 423 VGMKRRVGCLMLMNGGDEAELAGGPG--ATLGNYQQQGFEVVYDLEKNRVGFARRQCS 470
                     +  NG      AG  G  + +GN QQQGF VVYDL  +RVGFA   C+
Sbjct: 489 ----------VDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

The following BLAST results are available for this feature:

BLAST of MS012960.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023553227.1	6.5e-233	85.57	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]
KAG6577689.1	4.7e-231	84.95	putative aspartyl protease, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022923540.1	8.0e-231	84.95	probable aspartyl protease At4g16563 [Cucurbita moschata]	[more]
XP_023007805.1	2.3e-230	85.09	probable aspartyl protease At4g16563 [Cucurbita maxima]	[more]
XP_038905814.1	5.2e-230	84.71	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]

BLAST of MS012960.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	1.6e-165	60.77	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q766C3	7.2e-33	29.08	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
O04496	9.4e-33	28.61	Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1	[more]
Q9LNJ3	1.2e-32	31.82	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C2	2.6e-30	28.44	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]

BLAST of MS012960.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EC44	3.9e-231	84.95	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114312...	[more]
A0A6J1L3Z9	1.1e-230	85.09	probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111500303...	[more]
A0A1S3BK28	1.6e-229	84.65	aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103490888 PE=3 S...	[more]
A0A5D3CP11	2.8e-229	84.30	Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A0A0L5I7	3.6e-229	85.00	Pepsin A OS=Cucumis sativus OX=3659 GN=Csa_3G020060 PE=3 SV=1	[more]

BLAST of MS012960.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16563.1	1.1e-166	60.77	Eukaryotic aspartyl protease family protein	[more]
AT5G45120.1	4.9e-53	33.13	Eukaryotic aspartyl protease family protein	[more]
AT3G52500.1	3.2e-44	30.60	Eukaryotic aspartyl protease family protein	[more]
AT1G09750.1	6.7e-34	28.61	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	8.7e-34	31.82	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 75..257 e-value: 9.2E-28 score: 97.7
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 295..464 e-value: 2.0E-27 score: 96.0
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 273..474 e-value: 1.9E-46 score: 160.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 61..270 e-value: 2.6E-32 score: 114.3
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 69..473
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 2..473
None	No IPR available	PANTHER	PTHR47967:SF26	BNAA01G17170D PROTEIN	coord: 2..473
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 327..338
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 75..464 score: 34.331623
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 75..468 e-value: 2.10765E-61 score: 199.411

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS012960	MS012960	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS012960.1-cds	MS012960.1-cds-scaffold38:928166..929599	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS012960.1	MS012960.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity