MS012478.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS012478.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Cytochrome b-c1 complex subunit 6
Location	scaffold63: 992188 .. 993512 (+)
Sequence length	207
RNA-Seq Expression	MS012478.1
Synteny	MS012478.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGGCTGATGAGGAACCTGTTGATCAGAAAAGGTATCTCGAAGAAGCTTGCAAGCCCAAGTGCGTGAAACCACTACTTGAATATCAGGTACAGGGTGTTTTACTGGTCTTTACCATGGGGGATTAGCGATAGTTTAGTGAATTTGGATGTCTTTTTTTGTTTAGTTGTTGAACTTGTATTCATTATTCATTATTTCTTATGACCACTTAACTTTAAGAAGCTGGTTTCGTGAATAGCTATACTTCTAGTTCTCTCATTTAATTAGAAATTAAACCACTTCTAGTTTGAAGATTTTGCATCCCTCATGTAGAGTATAACCATTTATAAATGTTAAATGTTCTAATTTCTTCTGAGCCTTGCTTGTGATGCAACTATTAGTATTTAGTGAATTGTCGCTTCAACAGAAAGCCAAACTTTAAACGAGTGGCACCGTCGGTAGAAAATTAGTTAAAAACGAGTGAGAGTGTTTGGTGTTGGGAAGGATAATTGGATTTGGTGGTGGGCTGGTTCTTTTTGTTGAGAAGAAAAAAAATAAAATAAAAGCAGCCACCAACCATTAAGGTTTATGGGCATATTTGGAGAATATCATAATCCTACTTTTAAAGGTCACTGACACTCGTGGGGGAACATAAATATGTTGTCCTCGTGAGATTATGGTGAAAGATCCTCGGTTGTCATGCTAATCTCTTCGGTCATGGAGGTAATGATGGCCCTTGACAAATTGTCTTCTACGATACATAGGTCCTTGAGCTGTAAATTCAAATCAACATTGAGCACACGACTGAAGATGTCTTTTCTCCAGAATTTGATACATCTTATGACTCATTGATTTTGGTTGATATCTGCTCTGAGGTTCACGTTGACTCAGCCACTATCTGTCTTTTGATTGTGGCATTGATGAATCTCAAAATTCGTTGAACTTCTCTTTCTCCGTGAATACTAGTCCTCACCAAATTCGAAGAACTTATGCGTAGAGGTGCATGTGATTGTTTTCCAAAAGTCGAAAAATTTATCAAATCTCTTGCTTGTTTCTATGGGTTTCAGCTGAACTCTCTTCCCTGTTGACATTTAAGAACGAGGAGGATCTTAATATTTTTGCTTTGCTTATCATGTCTGAGCAGCAAGCTTATGTTATTCATGGATTCAAATTGTATTATAAGTATGAAGATAAGGTACAGTTATGGTTATTTTCTTTCATGTTATAGGCGTGCGTGAAGAGAATCCAAGGGGATGACAGTGGTCACAAGCATTGCACTGGACAGTATTTCGATTACTGGTTTTGCATCGACAAATGTGTAAGTATTTTTTGGGTTTCACAAAGAAGT

mRNA sequence

AGGGCTGATGAGGAACCTGTTGATCAGAAAAGGTATCTCGAAGAAGCTTGCAAGCCCAAGTGCGTGAAACCACTACTTGAATATCAGGCGTGCGTGAAGAGAATCCAAGGGGATGACAGTGGTCACAAGCATTGCACTGGACAGTATTTCGATTACTGGTTTTGCATCGACAAATGTGTAAGTATTTTTTGGGTTTCACAAAGAAGT

Coding sequence (CDS)

AGGGCTGATGAGGAACCTGTTGATCAGAAAAGGTATCTCGAAGAAGCTTGCAAGCCCAAGTGCGTGAAACCACTACTTGAATATCAGGCGTGCGTGAAGAGAATCCAAGGGGATGACAGTGGTCACAAGCATTGCACTGGACAGTATTTCGATTACTGGTTTTGCATCGACAAATGTGTAAGTATTTTTTGGGTTTCACAAAGAAGT

Protein sequence

RADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVSIFWVSQRS

Homology

[Close]

BLAST of MS012478.1 vs. NCBI nr
Match: XP_022149318.1 (cytochrome b-c1 complex subunit 6 [Momordica charantia])

HSP 1 Score: 137.1 bits (344), Expect = 5.7e-29
Identity = 59/60 (98.33%), Postives = 60/60 (100.00%), Query Frame = 0


Query: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
          ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCV+
Sbjct: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVA 61

[Close]

BLAST of MS012478.1 vs. NCBI nr
Match: XP_008456103.1 (PREDICTED: cytochrome b-c1 complex subunit 6 [Cucumis melo] >XP_008456104.1 PREDICTED: cytochrome b-c1 complex subunit 6 [Cucumis melo] >XP_038878290.1 cytochrome b-c1 complex subunit 6 [Benincasa hispida])

HSP 1 Score: 134.0 bits (336), Expect = 4.8e-28
Identity = 58/60 (96.67%), Postives = 59/60 (98.33%), Query Frame = 0


Query: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
          ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYW CIDKCV+
Sbjct: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWSCIDKCVA 61

[Close]

BLAST of MS012478.1 vs. NCBI nr
Match: KAE8650514.1 (hypothetical protein Csa_011326 [Cucumis sativus])

HSP 1 Score: 134.0 bits (336), Expect = 4.8e-28
Identity = 58/60 (96.67%), Postives = 59/60 (98.33%), Query Frame = 0


Query: 2   ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
           ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYW CIDKCV+
Sbjct: 52  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWSCIDKCVA 111

[Close]

BLAST of MS012478.1 vs. NCBI nr
Match: XP_004140689.1 (cytochrome b-c1 complex subunit 6 [Cucumis sativus])

HSP 1 Score: 134.0 bits (336), Expect = 4.8e-28
Identity = 58/60 (96.67%), Postives = 59/60 (98.33%), Query Frame = 0


Query: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
          ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYW CIDKCV+
Sbjct: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWSCIDKCVA 61

[Close]

BLAST of MS012478.1 vs. NCBI nr
Match: XP_022983580.1 (cytochrome b-c1 complex subunit 6-like [Cucurbita maxima])

HSP 1 Score: 133.7 bits (335), Expect = 6.3e-28
Identity = 57/60 (95.00%), Postives = 59/60 (98.33%), Query Frame = 0


Query: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
          ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYW C+DKCV+
Sbjct: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWSCVDKCVA 61

[Close]

BLAST of MS012478.1 vs. ExPASy Swiss-Prot
Match: Q0WWE3 (Cytochrome b-c1 complex subunit 6-1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=QCR6-1 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 5.0e-28
Identity = 53/60 (88.33%), Postives = 57/60 (95.00%), Query Frame = 0


Query: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
          AD+E VD K+YLEE+CKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYW CIDKCV+
Sbjct: 2  ADDEVVDPKKYLEESCKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWQCIDKCVA 61

[Close]

BLAST of MS012478.1 vs. ExPASy Swiss-Prot
Match: Q9SJV7 (Cytochrome b-c1 complex subunit 6-2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=QCR6-2 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 3.2e-27
Identity = 50/60 (83.33%), Postives = 56/60 (93.33%), Query Frame = 0


Query: 3  DEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVSI 62
          +E+ VDQKRY EE+CKPKCVKPLLEYQACVKRIQ D+SGHKHCTGQYFDYW C+DKCVS+
Sbjct: 3  EEDVVDQKRYFEESCKPKCVKPLLEYQACVKRIQDDESGHKHCTGQYFDYWHCVDKCVSV 62

[Close]

BLAST of MS012478.1 vs. ExPASy Swiss-Prot
Match: P48504 (Cytochrome b-c1 complex subunit 6 OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 106.7 bits (265), Expect = 1.1e-22
Identity = 44/60 (73.33%), Postives = 51/60 (85.00%), Query Frame = 0


Query: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
          +DEE VD K  LE +CKPKCV+ L EYQAC KR++GD+SGHKHCTGQYFDYW CIDKCV+
Sbjct: 2  SDEEVVDPKATLEVSCKPKCVRQLKEYQACTKRVEGDESGHKHCTGQYFDYWHCIDKCVA 61

[Close]

BLAST of MS012478.1 vs. ExPASy TrEMBL
Match: A0A6J1D6H2 (Cytochrome b-c1 complex subunit 6 OS=Momordica charantia OX=3673 GN=LOC111017759 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.7e-29
Identity = 59/60 (98.33%), Postives = 60/60 (100.00%), Query Frame = 0


Query: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
          ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCV+
Sbjct: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVA 61

[Close]

BLAST of MS012478.1 vs. ExPASy TrEMBL
Match: A0A1S3C350 (Cytochrome b-c1 complex subunit 6 OS=Cucumis melo OX=3656 GN=LOC103496141 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.3e-28
Identity = 58/60 (96.67%), Postives = 59/60 (98.33%), Query Frame = 0


Query: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
          ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYW CIDKCV+
Sbjct: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWSCIDKCVA 61

[Close]

BLAST of MS012478.1 vs. ExPASy TrEMBL
Match: A0A0A0L6I2 (UCR_hinge domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G212480 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.3e-28
Identity = 58/60 (96.67%), Postives = 59/60 (98.33%), Query Frame = 0


Query: 2   ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
           ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYW CIDKCV+
Sbjct: 61  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWSCIDKCVA 120

[Close]

BLAST of MS012478.1 vs. ExPASy TrEMBL
Match: A0A6J1J694 (Cytochrome b-c1 complex subunit 6 OS=Cucurbita maxima OX=3661 GN=LOC111482139 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 3.0e-28
Identity = 57/60 (95.00%), Postives = 59/60 (98.33%), Query Frame = 0


Query: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
          ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYW C+DKCV+
Sbjct: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWSCVDKCVA 61

[Close]

BLAST of MS012478.1 vs. ExPASy TrEMBL
Match: A0A6J1FRR8 (cytochrome b-c1 complex subunit 6-like OS=Cucurbita moschata OX=3662 GN=LOC111447856 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 3.0e-28
Identity = 57/60 (95.00%), Postives = 59/60 (98.33%), Query Frame = 0


Query: 2   ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
           ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYW C+DKCV+
Sbjct: 67  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWSCVDKCVA 126

[Close]

BLAST of MS012478.1 vs. TAIR 10
Match: AT1G15120.1 (Ubiquinol-cytochrome C reductase hinge protein )

HSP 1 Score: 124.4 bits (311), Expect = 3.5e-29
Identity = 53/60 (88.33%), Postives = 57/60 (95.00%), Query Frame = 0


Query: 2  ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVS 61
          AD+E VD K+YLEE+CKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYW CIDKCV+
Sbjct: 2  ADDEVVDPKKYLEESCKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWQCIDKCVA 61

[Close]

BLAST of MS012478.1 vs. TAIR 10
Match: AT1G15120.2 (Ubiquinol-cytochrome C reductase hinge protein )

HSP 1 Score: 122.1 bits (305), Expect = 1.8e-28
Identity = 52/58 (89.66%), Postives = 55/58 (94.83%), Query Frame = 0


Query: 2   ADEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKC 60
           AD+E VD K+YLEE+CKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYW CIDKC
Sbjct: 69  ADDEVVDPKKYLEESCKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWQCIDKC 126

[Close]

BLAST of MS012478.1 vs. TAIR 10
Match: AT2G01090.1 (Ubiquinol-cytochrome C reductase hinge protein )

HSP 1 Score: 121.7 bits (304), Expect = 2.3e-28
Identity = 50/60 (83.33%), Postives = 56/60 (93.33%), Query Frame = 0


Query: 3  DEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVSI 62
          +E+ VDQKRY EE+CKPKCVKPLLEYQACVKRIQ D+SGHKHCTGQYFDYW C+DKCVS+
Sbjct: 3  EEDVVDQKRYFEESCKPKCVKPLLEYQACVKRIQDDESGHKHCTGQYFDYWHCVDKCVSV 62

[Close]

BLAST of MS012478.1 vs. TAIR 10
Match: AT2G01090.2 (Ubiquinol-cytochrome C reductase hinge protein )

HSP 1 Score: 121.7 bits (304), Expect = 2.3e-28
Identity = 50/60 (83.33%), Postives = 56/60 (93.33%), Query Frame = 0


Query: 3  DEEPVDQKRYLEEACKPKCVKPLLEYQACVKRIQGDDSGHKHCTGQYFDYWFCIDKCVSI 62
          +E+ VDQKRY EE+CKPKCVKPLLEYQACVKRIQ D+SGHKHCTGQYFDYW C+DKCVS+
Sbjct: 3  EEDVVDQKRYFEESCKPKCVKPLLEYQACVKRIQDDESGHKHCTGQYFDYWHCVDKCVSV 62

The following BLAST results are available for this feature:

BLAST of MS012478.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149318.1	5.7e-29	98.33	cytochrome b-c1 complex subunit 6 [Momordica charantia]	[more]
XP_008456103.1	4.8e-28	96.67	PREDICTED: cytochrome b-c1 complex subunit 6 [Cucumis melo] >XP_008456104.1 PRED...	[more]
KAE8650514.1	4.8e-28	96.67	hypothetical protein Csa_011326 [Cucumis sativus]	[more]
XP_004140689.1	4.8e-28	96.67	cytochrome b-c1 complex subunit 6 [Cucumis sativus]	[more]
XP_022983580.1	6.3e-28	95.00	cytochrome b-c1 complex subunit 6-like [Cucurbita maxima]	[more]

BLAST of MS012478.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WWE3	5.0e-28	88.33	Cytochrome b-c1 complex subunit 6-1, mitochondrial OS=Arabidopsis thaliana OX=37...	[more]
Q9SJV7	3.2e-27	83.33	Cytochrome b-c1 complex subunit 6-2, mitochondrial OS=Arabidopsis thaliana OX=37...	[more]
P48504	1.1e-22	73.33	Cytochrome b-c1 complex subunit 6 OS=Solanum tuberosum OX=4113 PE=1 SV=2	[more]

BLAST of MS012478.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D6H2	2.7e-29	98.33	Cytochrome b-c1 complex subunit 6 OS=Momordica charantia OX=3673 GN=LOC111017759...	[more]
A0A1S3C350	2.3e-28	96.67	Cytochrome b-c1 complex subunit 6 OS=Cucumis melo OX=3656 GN=LOC103496141 PE=3 S...	[more]
A0A0A0L6I2	2.3e-28	96.67	UCR_hinge domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G212480 P...	[more]
A0A6J1J694	3.0e-28	95.00	Cytochrome b-c1 complex subunit 6 OS=Cucurbita maxima OX=3661 GN=LOC111482139 PE...	[more]
A0A6J1FRR8	3.0e-28	95.00	cytochrome b-c1 complex subunit 6-like OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of MS012478.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15120.1	3.5e-29	88.33	Ubiquinol-cytochrome C reductase hinge protein	[more]
AT1G15120.2	1.8e-28	89.66	Ubiquinol-cytochrome C reductase hinge protein	[more]
AT2G01090.1	2.3e-28	83.33	Ubiquinol-cytochrome C reductase hinge protein	[more]
AT2G01090.2	2.3e-28	83.33	Ubiquinol-cytochrome C reductase hinge protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003422	Cytochrome b-c1 complex, subunit 6	PIRSF	PIRSF000019	Bc1_11K	coord: 1..66 e-value: 6.2E-32 score: 107.5
IPR003422	Cytochrome b-c1 complex, subunit 6	PANTHER	PTHR15336	UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 7.8 KDA PROTEIN	coord: 1..61
IPR023184	Ubiquinol-cytochrome C reductase hinge domain	PFAM	PF02320	UCR_hinge	coord: 8..61 e-value: 3.9E-17 score: 62.2
IPR036811	Ubiquinol-cytochrome C reductase hinge domain superfamily	GENE3D	1.10.287.20		coord: 1..65 e-value: 2.8E-19 score: 70.9
IPR036811	Ubiquinol-cytochrome C reductase hinge domain superfamily	SUPERFAMILY	81531	Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)	coord: 2..61
None	No IPR available	PANTHER	PTHR15336:SF14	CYTOCHROME B-C1 COMPLEX SUBUNIT 6	coord: 1..61

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS012478	MS012478	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS012478.1-cds	MS012478.1-cds-scaffold63:992188..992274	CDS
MS012478.1-cds	MS012478.1-cds-scaffold63:993393..993512	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS012478.1	MS012478.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006122	mitochondrial electron transport, ubiquinol to cytochrome c
cellular_component	GO:0005750	mitochondrial respiratory chain complex III