Homology
BLAST of MS009497.1 vs. NCBI nr
Match:
XP_022152954.1 (malonate--CoA ligase isoform X1 [Momordica charantia] >XP_022152963.1 malonate--CoA ligase isoform X1 [Momordica charantia])
HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 587/606 (96.86%), Postives = 589/606 (97.19%), Query Frame = 0
Query: 1 MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT
Sbjct: 1 MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
Query: 61 FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHG+LGGAK
Sbjct: 61 FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGNLGGAK 120
Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ
Sbjct: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT 240
SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT
Sbjct: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT 240
Query: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVH 300
TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH HVH
Sbjct: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH-----------------HVH 300
Query: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL 360
GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL
Sbjct: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL 360
Query: 361 QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI 420
QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI
Sbjct: 361 QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI 420
Query: 421 SNPLNGERKVGTVGKPFPGIEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLD 480
SNPLNGERKVGTVGKPFPG+EAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLD
Sbjct: 421 SNPLNGERKVGTVGKPFPGVEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLD 480
Query: 481 GGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLP 540
GGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLP
Sbjct: 481 GGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLP 540
Query: 541 DKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPR 600
DKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPR
Sbjct: 541 DKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPR 589
Query: 601 NAMGKV 607
NAMGKV
Sbjct: 601 NAMGKV 589
BLAST of MS009497.1 vs. NCBI nr
Match:
XP_022152972.1 (malonate--CoA ligase isoform X2 [Momordica charantia])
HSP 1 Score: 1044.6 bits (2700), Expect = 3.2e-301
Identity = 526/545 (96.51%), Postives = 528/545 (96.88%), Query Frame = 0
Query: 62 MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAKV 121
MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHG+LGGAKV
Sbjct: 1 MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGNLGGAKV 60
Query: 122 GIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQS 181
GIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQS
Sbjct: 61 GIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQS 120
Query: 182 VAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTT 241
VAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTT
Sbjct: 121 VAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTT 180
Query: 242 GKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVHG 301
GKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH HVHG
Sbjct: 181 GKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH-----------------HVHG 240
Query: 302 LFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQ 361
LFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQ
Sbjct: 241 LFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQ 300
Query: 362 GYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAIS 421
GYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAIS
Sbjct: 301 GYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAIS 360
Query: 422 NPLNGERKVGTVGKPFPGIEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLDG 481
NPLNGERKVGTVGKPFPG+EAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLDG
Sbjct: 361 NPLNGERKVGTVGKPFPGVEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLDG 420
Query: 482 GFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPD 541
GFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPD
Sbjct: 421 GFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPD 480
Query: 542 KDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRN 601
KDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRN
Sbjct: 481 KDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRN 528
Query: 602 AMGKV 607
AMGKV
Sbjct: 541 AMGKV 528
BLAST of MS009497.1 vs. NCBI nr
Match:
XP_038904615.1 (malonate--CoA ligase isoform X1 [Benincasa hispida])
HSP 1 Score: 984.9 bits (2545), Expect = 3.0e-283
Identity = 500/613 (81.57%), Postives = 538/613 (87.77%), Query Frame = 0
Query: 1 MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
MNSSTF + T F RIC PS LI RS S C++LGR+ +L F R RL+S++SS+T
Sbjct: 1 MNSSTFNNLTSLFTRICHLSLPPSHLIAIRSTSACFHLGRNTPQLCFSRFRLFSVLSSYT 60
Query: 61 FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
FMEVVKS AKHGST E+VAIRANQKSHSY QLISSALKIS LLTSS+++T H HLGGAK
Sbjct: 61 FMEVVKSAAKHGSTCRENVAIRANQKSHSYIQLISSALKISDLLTSSNLRTGHAHLGGAK 120
Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
+GIVAKPSAEFLAGVLGTW SGGVAVPLALSYPEAELLHVLNDSEVSMILSTED EFMQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180
Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIE---ADADRIFQGTIENLSDDPALIIYT 240
SVAAKT A SLIPPVPHI SERN H+Q TDIE ADADR+FQGT+ENLSDDPALIIYT
Sbjct: 181 SVAAKTVATVSLIPPVPHIYSERNSHDQSTDIEVDAADADRVFQGTVENLSDDPALIIYT 240
Query: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNL 300
SGTTGKPKGVVHTHKSITSQV TLS AWEYTSADQFLHCLPLH
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVHTLSNAWEYTSADQFLHCLPLH----------------- 300
Query: 301 HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYA 360
HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPS SSKSDDAITVFTGVPTMYA
Sbjct: 301 HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYA 360
Query: 361 RLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFV 420
RLLQGYEAMD ELQ+ASASAA+QLRLMMCGSSALPPP+M+QW++VTGH LLERYGMTEFV
Sbjct: 361 RLLQGYEAMDSELQLASASAARQLRLMMCGSSALPPPIMKQWKEVTGHELLERYGMTEFV 420
Query: 421 MAISNPLNGERKVGTVGKPFPGIEAKIVADDESSN----EGELCIRSPSMFKEYWKLPEV 480
MAISNPLNG+RK+GTVGKPFPG+EAKIVADD S+N EGELCIRSPSMFKEYWKLPEV
Sbjct: 421 MAISNPLNGQRKIGTVGKPFPGVEAKIVADDGSTNDSSLEGELCIRSPSMFKEYWKLPEV 480
Query: 481 TKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIE 540
TKESF+DGGFFKTGDAAR DE+GYYIILGR++ADIMKVGGYKLSALEIESVILQHPSV+E
Sbjct: 481 TKESFIDGGFFKTGDAARLDEDGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVVE 540
Query: 541 CCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLL 600
CCVLGLPDKDYGERVCAIIVL+PN K T PD+S P ++L ELRTWA+DKLAPYKLPT LL
Sbjct: 541 CCVLGLPDKDYGERVCAIIVLQPNTKTTTPDESNPTMSLDELRTWAKDKLAPYKLPTMLL 596
Query: 601 LWESLPRNAMGKV 607
L +SLPRNAMGKV
Sbjct: 601 LKDSLPRNAMGKV 596
BLAST of MS009497.1 vs. NCBI nr
Match:
XP_023515293.1 (malonate--CoA ligase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 981.1 bits (2535), Expect = 4.3e-282
Identity = 493/610 (80.82%), Postives = 533/610 (87.38%), Query Frame = 0
Query: 1 MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
MNS TF T+ F RIC FAPS LI +S S C L RSI K+ FPRSRL+S+ SS T
Sbjct: 1 MNSFTFNYSTYLFRRICHLSFAPSHLIAIQSCSACLRLHRSIPKICFPRSRLFSVSSSST 60
Query: 61 FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
FMEVVKS AKHGST E+VAIRAN KSHSY QLISSALKIS L TS D++T HGHL GAK
Sbjct: 61 FMEVVKSAAKHGSTCRENVAIRANMKSHSYMQLISSALKISDLFTSCDLRTGHGHLDGAK 120
Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
+GI+AKPSAEFLAG+LGTW SGGVAVPLAL+YPE ELLHVLNDSEVSMILSTED E MQ
Sbjct: 121 IGIIAKPSAEFLAGLLGTWFSGGVAVPLALNYPEPELLHVLNDSEVSMILSTEDHAEIMQ 180
Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT 240
SVAAKTAAKFSLIPPVPHI SE N +Q TDIE DA+R+FQGT+ENLSDDPAL+IYTSGT
Sbjct: 181 SVAAKTAAKFSLIPPVPHIYSETNSRDQSTDIEMDAERVFQGTVENLSDDPALVIYTSGT 240
Query: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVH 300
TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQ LHCLPLH HVH
Sbjct: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQLLHCLPLH-----------------HVH 300
Query: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL 360
GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSN SK+DDAIT+FTGVPTMYARLL
Sbjct: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNGSKNDDAITIFTGVPTMYARLL 360
Query: 361 QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI 420
QGYEAM+P+LQVASA+AA+QLRLMMCGSSALPPP+M+QW+ VTGH+LLERYGMTEFVMAI
Sbjct: 361 QGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMTEFVMAI 420
Query: 421 SNPLNGERKVGTVGKPFPGIEAKIVADDESSN----EGELCIRSPSMFKEYWKLPEVTKE 480
SNPLNGERK+GTVGKP PG+EAKIV+DD S+N EGELCIRSPSMFKEYWKLP+VTKE
Sbjct: 421 SNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKE 480
Query: 481 SFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCV 540
SF+DGGFFKTGDAAR DE+GYY ILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCV
Sbjct: 481 SFIDGGFFKTGDAARLDEDGYYTILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCV 540
Query: 541 LGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWE 600
LGLPDKDYGERVCAIIVL PN K TPPD SKP ++L +LRTWA+DKLAPYKLPT+LLL +
Sbjct: 541 LGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPTELLLKD 593
Query: 601 SLPRNAMGKV 607
SLPRNAMGKV
Sbjct: 601 SLPRNAMGKV 593
BLAST of MS009497.1 vs. NCBI nr
Match:
XP_008452304.1 (PREDICTED: malonate--CoA ligase isoform X1 [Cucumis melo])
HSP 1 Score: 976.9 bits (2524), Expect = 8.2e-281
Identity = 498/613 (81.24%), Postives = 535/613 (87.28%), Query Frame = 0
Query: 1 MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
MNSS F +PT F RIC S LI RS S C+ +G+SI K F RSRL+S++SS+T
Sbjct: 1 MNSSPFNNPTSLFTRICHLSLPSSHLIAIRSSSACFCIGKSIPKFCFSRSRLFSVLSSYT 60
Query: 61 FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
FMEVVKS AKHG+T ESVAIRANQKSHSY QLISSALKIS LLTSSD++T HGHLGGAK
Sbjct: 61 FMEVVKSAAKHGATCRESVAIRANQKSHSYIQLISSALKISDLLTSSDLRTGHGHLGGAK 120
Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
+GIVAKPSAEFLAGVLGTW SGGVAVPLALSYPEAELLHVLNDSEVSMILSTED EFMQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180
Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIE---ADADRIFQGTIENLSDDPALIIYT 240
SVAAKT A SLIPPVPHI SERN H+Q T+IE ADADR+FQGT+ENLSDDPALIIYT
Sbjct: 181 SVAAKTVAIVSLIPPVPHIYSERNSHDQSTNIEVNAADADRVFQGTVENLSDDPALIIYT 240
Query: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNL 300
SGTTGKPKGVVHTHKSITSQVQTLS AWEYTSADQFLHCLPLH
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQTLSNAWEYTSADQFLHCLPLH----------------- 300
Query: 301 HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYA 360
HVHG+FNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPS SSKSDDAITVFTGVPTMYA
Sbjct: 301 HVHGMFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYA 360
Query: 361 RLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFV 420
RLLQGYEAMD ELQ+ASASAA+QLRLMMCGSSALPPP+M+QW +VTGH LLERYGMTEFV
Sbjct: 361 RLLQGYEAMDSELQLASASAARQLRLMMCGSSALPPPIMKQWREVTGHELLERYGMTEFV 420
Query: 421 MAISNPLNGERKVGTVGKPFPGIEAKIVADD----ESSNEGELCIRSPSMFKEYWKLPEV 480
MAISNPLNG+RK+GTVGKPFPG+EAKIVADD SS EGELCIRSPSMFKEYWKLP+V
Sbjct: 421 MAISNPLNGQRKIGTVGKPFPGVEAKIVADDGSTTGSSLEGELCIRSPSMFKEYWKLPKV 480
Query: 481 TKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIE 540
TKESF+DGGFFKTGDAAR DE+GYYIILGR++ADIMKVGGYKLSALEIESVILQHPSV E
Sbjct: 481 TKESFIDGGFFKTGDAARLDEDGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVTE 540
Query: 541 CCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLL 600
CCVLGLPDKDYGERVCAI+VL+ N K T PD S P+++L ELRTWA+DKLAPYKLPT LL
Sbjct: 541 CCVLGLPDKDYGERVCAIVVLQANTKTTIPDGSNPSMSLDELRTWAKDKLAPYKLPTMLL 596
Query: 601 LWESLPRNAMGKV 607
L +SLPRNAMGKV
Sbjct: 601 LKDSLPRNAMGKV 596
BLAST of MS009497.1 vs. ExPASy Swiss-Prot
Match:
M4IS90 (Probable CoA ligase CCL8 OS=Humulus lupulus OX=3486 GN=CCL8 PE=2 SV=1)
HSP 1 Score: 773.9 bits (1997), Expect = 1.4e-222
Identity = 400/582 (68.73%), Postives = 459/582 (78.87%), Query Frame = 0
Query: 48 PRSRLYSIISSHTFMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSS 107
P S L+S ++ ME++K AK GS+ S VAIRA+QKS+SY QLISSA +I +LL S+
Sbjct: 31 PFSSLHSGSANLILMELLKEVAKEGSSASVGVAIRADQKSYSYKQLISSAQRICSLLCST 90
Query: 108 DIKTE-----------------HGHLGGAKVGIVAKPSAEFLAGVLGTWLSGGVAVPLAL 167
D+K HGHLGGA+VGIVAKPSAEF+ GVLGTWLSGGVAVPLAL
Sbjct: 91 DLKASHKAGKQVTPALINGLSGHGHLGGARVGIVAKPSAEFVTGVLGTWLSGGVAVPLAL 150
Query: 168 SYPEAELLHVLNDSEVSMILSTEDRGEFMQSVAAKTAAKFSLIPPVPHISSERN--PHEQ 227
SYPEAELLHV+ DS++SMILSTED E MQ +AAKTAA+FSLIPPVP S+ H Q
Sbjct: 151 SYPEAELLHVMTDSDISMILSTEDHQELMQKIAAKTAAQFSLIPPVPSSCSQEGAVDHLQ 210
Query: 228 PTDIEADADRIFQGTIENLSDDPALIIYTSGTTGKPKGVVHTHKSITSQVQTLSKAWEYT 287
DI D+ I T E +++PALI+YTSGTTGKPKGVVHTHKSI +QVQTL+KAWEYT
Sbjct: 211 TGDINTDS--ILHNT-EISNENPALIVYTSGTTGKPKGVVHTHKSINAQVQTLAKAWEYT 270
Query: 288 SADQFLHCLPLHHILEFSTFVAVSPYFNLHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIW 347
ADQFLHCLPLH HVHGLFN L AP+YA STVEFMPKFSV GIW
Sbjct: 271 PADQFLHCLPLH-----------------HVHGLFNALFAPLYARSTVEFMPKFSVRGIW 330
Query: 348 KRWRESYPSNSSKSDDAITVFTGVPTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGS 407
+RWRESYP+ +K DD ITVFTGVPTMY RL+QGYEAMDPEL+ ASASAAKQLRLMMCGS
Sbjct: 331 QRWRESYPTCETKVDDCITVFTGVPTMYTRLIQGYEAMDPELKEASASAAKQLRLMMCGS 390
Query: 408 SALPPPVMQQWEDVTGHRLLERYGMTEFVMAISNPLNGERKVGTVGKPFPGIEAKIVADD 467
SALP PVMQQW+ +TGHRLLERYGMTEFVMAISNPL GERK GTVGKPFPG+E +IVA+D
Sbjct: 391 SALPIPVMQQWQTITGHRLLERYGMTEFVMAISNPLKGERKAGTVGKPFPGVEVRIVAED 450
Query: 468 ESSNE----GELCIRSPSMFKEYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRM 527
E+ ++ GELC+RSPS+FKEYW+LPEVTK SF D GFFKTGDA + DE+GYY+ILGR
Sbjct: 451 ENGSDTTGVGELCVRSPSLFKEYWRLPEVTKSSFTDDGFFKTGDAGKVDEDGYYVILGRT 510
Query: 528 NADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPD 587
+ADIMKVGGYKLSALEIESV+L+HP+V ECCVLGLPDKDYGE V AIIV K+ +
Sbjct: 511 SADIMKVGGYKLSALEIESVLLEHPTVEECCVLGLPDKDYGEAVSAIIVPAAEAKKKGEE 570
Query: 588 DSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRNAMGKV 607
+SKPAI+L EL +WA+ KLAPYKLPT+L LW+SLPRNAMGKV
Sbjct: 571 ESKPAISLEELFSWAQHKLAPYKLPTRLFLWDSLPRNAMGKV 592
BLAST of MS009497.1 vs. ExPASy Swiss-Prot
Match:
Q8H151 (Malonate--CoA ligase OS=Arabidopsis thaliana OX=3702 GN=AAE13 PE=1 SV=2)
HSP 1 Score: 713.4 bits (1840), Expect = 2.2e-204
Identity = 366/571 (64.10%), Postives = 430/571 (75.31%), Query Frame = 0
Query: 46 RFPRSRLYSIISSHTFMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLT 105
RF +S S + MEV K+ S + +AI+A+ KS+SYGQL SSAL+IS L
Sbjct: 49 RFFQSNHLFSSQSGSLMEVFKAAFSEASNSCDRIAIKADGKSYSYGQLTSSALRISKLFL 108
Query: 106 SSDIKT---------EHGHLGGAKVGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAE 165
D G L GA++GIVAKPSAEF+AGVLGTW SGGVAVPLALSYPEAE
Sbjct: 109 KDDTTNGGQETKKYEGFGSLKGARIGIVAKPSAEFVAGVLGTWFSGGVAVPLALSYPEAE 168
Query: 166 LLHVLNDSEVSMILSTEDRGEFMQSVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADA 225
LLHV+NDS++S++LSTED E M+++AAK+ A+F LIPPV + +SE Q D +A
Sbjct: 169 LLHVMNDSDISLLLSTEDHSETMKTIAAKSGARFHLIPPVVNSTSETVACNQFQDDSFEA 228
Query: 226 DRIFQGTIENLSDDPALIIYTSGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHC 285
+ F DDPALI+YTSGTTGKPKGVVHTH SI SQV+ L++AWEYTSAD FLHC
Sbjct: 229 EGKF-------LDDPALIVYTSGTTGKPKGVVHTHNSINSQVRMLTEAWEYTSADHFLHC 288
Query: 286 LPLHHILEFSTFVAVSPYFNLHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYP 345
LPLH HVHGLFN L AP+YA S VEF+PKFSVSGIW+RWRESYP
Sbjct: 289 LPLH-----------------HVHGLFNALFAPLYARSLVEFLPKFSVSGIWRRWRESYP 348
Query: 346 SNSSKSDDAITVFTGVPTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVM 405
N K++D+ITVFTGVPTMY RL+QGYEAMD E+Q +SA AA++LRLMM GSSALP PVM
Sbjct: 349 VNDEKTNDSITVFTGVPTMYTRLIQGYEAMDKEMQDSSAFAARKLRLMMSGSSALPRPVM 408
Query: 406 QQWEDVTGHRLLERYGMTEFVMAISNPLNGERKVGTVGKPFPGIEAKIVADDESSN-EGE 465
QWE +TGHRLLERYGMTEFVMA+SNPL G R GTVGKP PG+EAKI D+ +N GE
Sbjct: 409 HQWESITGHRLLERYGMTEFVMAMSNPLRGARNAGTVGKPLPGVEAKIKEDENDANGVGE 468
Query: 466 LCIRSPSMFKEYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYK 525
+C++SPS+FKEYW LPEVTKESF + G+FKTGDA R DE+GYY+ILGR +ADIMKVGGYK
Sbjct: 469 ICVKSPSLFKEYWNLPEVTKESFTEDGYFKTGDAGRVDEDGYYVILGRNSADIMKVGGYK 528
Query: 526 LSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLEL 585
LSALEIES +L+HP+V ECCVLGL D DYGE V AII+ E K+ D+SKP ITL EL
Sbjct: 529 LSALEIESTLLEHPTVAECCVLGLTDNDYGEAVTAIIIAESAAKKRREDESKPVITLEEL 588
Query: 586 RTWARDKLAPYKLPTKLLLWESLPRNAMGKV 607
WA+DKLAPYKLPT+LL+WESLPRNAMGKV
Sbjct: 589 CGWAKDKLAPYKLPTRLLIWESLPRNAMGKV 595
BLAST of MS009497.1 vs. ExPASy Swiss-Prot
Match:
Q58DN7 (Malonate--CoA ligase ACSF3, mitochondrial OS=Bos taurus OX=9913 GN=ACSF3 PE=2 SV=1)
HSP 1 Score: 351.7 bits (901), Expect = 1.7e-95
Identity = 205/561 (36.54%), Postives = 302/561 (53.83%), Query Frame = 0
Query: 75 FSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAKVGIVAKPSAEFLAG 134
F + +A+ H+Y L +L++S + + G L +V ++ F+
Sbjct: 54 FGDRLALIDQHGRHTYKDLYLRSLRLSREICQLRACAD-GDLREERVSLLCSNDVSFVVA 113
Query: 135 VLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQSVAAKTAAKFSLIP 194
W+SGGVAVPL +P A+L + + DS S++L+ + E + VA K +P
Sbjct: 114 QWAAWMSGGVAVPLYRKHPRAQLEYFIQDSRSSVVLAGPEHVELLSPVAQKLGVPLLPLP 173
Query: 195 PVPHISSERNPHEQPTDIEADADRIFQGTI--ENLSDDPALIIYTSGTTGKPKGVVHTHK 254
P + +P E G + N D A+IIYTSGTTG+PKGV+ TH
Sbjct: 174 PTVYHGVAEDPEE--------------GLVLERNWRDRGAMIIYTSGTTGRPKGVLSTHD 233
Query: 255 SITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVHGLFNGLLAPIYA 314
+I + V L W +T D LH LPLH HVHG+ N LL P++
Sbjct: 234 NIRAVVTGLVHKWAWTKDDVILHVLPLH-----------------HVHGVVNKLLCPLWV 293
Query: 315 GSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQGYEA--MDPEL 374
G+T +P+FS +W+++ S I VF VPT+Y++L+ Y+ P +
Sbjct: 294 GATCVMLPEFSAQLVWEKF-------LSSEAPQINVFMAVPTIYSKLMDYYDKHFTQPHV 353
Query: 375 Q-VASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAISNPLNGERK 434
Q A +++RLM+ GS+ALP PV+++W+ +TGH LLERYGMTE MA+SNPL R
Sbjct: 354 QDFVRAVCEEKIRLMVSGSAALPLPVLEKWKGITGHTLLERYGMTEIGMALSNPLTAARL 413
Query: 435 VGTVGKPFPGIEAKIVADDESSN------------------------EGELCIRSPSMFK 494
G+VG P PG+E +IV+++ + EGEL +R PS+F+
Sbjct: 414 PGSVGTPLPGVEVRIVSENPQKDSSPYLIHAEGSEENTKVTPGFEEKEGELLVRGPSVFR 473
Query: 495 EYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVI 554
EYW PE TK +F G+FKTGD ++G Y I GR + DI+K GGYK+SALE+E ++
Sbjct: 474 EYWDKPEETKAAFTSDGWFKTGDTV-VFKDGCYWIRGRTSVDIIKSGGYKVSALEVERLL 533
Query: 555 LQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAP 607
L HPS+ + V+G+PD +G+RV A++ L+ +++ EL+ WAR LAP
Sbjct: 534 LAHPSITDVAVIGVPDMTWGQRVTAVVTLQEGH----------SLSHRELKEWARGVLAP 564
BLAST of MS009497.1 vs. ExPASy Swiss-Prot
Match:
Q3URE1 (Malonate--CoA ligase ACSF3, mitochondrial OS=Mus musculus OX=10090 GN=Acsf3 PE=1 SV=2)
HSP 1 Score: 344.4 bits (882), Expect = 2.7e-93
Identity = 198/558 (35.48%), Postives = 298/558 (53.41%), Query Frame = 0
Query: 75 FSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAKVGIVAKPSAEFLAG 134
F + +A+ H+Y +L +L ++ + + G L +V + ++
Sbjct: 53 FGDRIALIDKYGHHTYRELYDRSLCLAQEICRLQ-GCKVGDLQEERVSFLCSNDVSYVVA 112
Query: 135 VLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQSVAAKTAAKFSLIP 194
+W+SGGVAVPL +PEA+L + + DS S+++ ++ E + +A + +
Sbjct: 113 QWASWMSGGVAVPLYWKHPEAQLEYFIQDSRSSLVVVGQEYLERLSPLAQRLGVPLLPLT 172
Query: 195 PVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTTGKPKGVVHTHKSI 254
P + + P EQP + DR A+I YTSGTTG+PKG + TH+++
Sbjct: 173 PAVYHGATEKPTEQPVEESGWRDR------------GAMIFYTSGTTGRPKGALSTHRNL 232
Query: 255 TSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVHGLFNGLLAPIYAGS 314
+ V L +W +T D LH LPLH HVHG+ N LL P++ G+
Sbjct: 233 AAVVTGLVHSWAWTKNDVILHVLPLH-----------------HVHGVVNKLLCPLWVGA 292
Query: 315 TVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQGYEA--MDPELQ- 374
T +P+FS +W+++ S ITVF VPT+Y++LL Y+ P +Q
Sbjct: 293 TCVMLPEFSAQQVWEKF-------LSSEAPQITVFMAVPTVYSKLLDYYDKHFTQPHVQD 352
Query: 375 VASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAISNPLNGERKVG 434
A +++RLM+ GS+ALP P++++W TGH LLERYGMTE MA+SNPL R G
Sbjct: 353 FVRAVCKERIRLMVSGSAALPVPLLEKWRSATGHTLLERYGMTEIGMALSNPLTEARVPG 412
Query: 435 TVGKPFPGIEAKIVADDE-----------------------SSNEGELCIRSPSMFKEYW 494
+VG P PG+E +I++++ EGEL +R PS+F+EYW
Sbjct: 413 SVGTPLPGVEVRIISENPQKGSPYIIHAEGNERGTKVTPGFEEKEGELLVRGPSVFREYW 472
Query: 495 KLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQH 554
PE TK +F G+F+TGD A + Y+ I GR + DI+K GGYK+SALEIE +L H
Sbjct: 473 DKPEETKSAFTSDGWFRTGDTAVFKDARYW-IRGRTSVDIIKTGGYKVSALEIERHLLAH 532
Query: 555 PSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKL 607
PS+ + V+G+PD +G+RV A++ L+ + D L+ WAR LAPY +
Sbjct: 533 PSITDVAVIGVPDMTWGQRVTAVVALQEGHSLSHGD----------LKEWARGVLAPYAV 562
BLAST of MS009497.1 vs. ExPASy Swiss-Prot
Match:
Q4G176 (Malonate--CoA ligase ACSF3, mitochondrial OS=Homo sapiens OX=9606 GN=ACSF3 PE=1 SV=3)
HSP 1 Score: 342.4 bits (877), Expect = 1.0e-92
Identity = 204/564 (36.17%), Postives = 308/564 (54.61%), Query Frame = 0
Query: 75 FSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGG----AKVGIVAKPSAE 134
F + +A+ H+Y +L S +L++ S +I G +GG +V + A
Sbjct: 53 FGDRIALVDQHGRHTYRELYSRSLRL-----SQEICRLCGCVGGDLREERVSFLCANDAS 112
Query: 135 FLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQSVAAKTAAKF 194
++ +W+SGGVAVPL +P A+L +V+ DS+ S++L++++ E + V K
Sbjct: 113 YVVAQWASWMSGGVAVPLYRKHPAAQLEYVICDSQSSVVLASQEYLELLSPVVRKLGVPL 172
Query: 195 SLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTTGKPKGVVHT 254
+ P + + P E P + ++ A+IIYTSGTTG+PKGV+ T
Sbjct: 173 LPLTPAIYTGAVEEPAEVPVPEQGWRNK------------GAMIIYTSGTTGRPKGVLST 232
Query: 255 HKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVHGLFNGLLAPI 314
H++I + V L W +T D LH LPLH HVHG+ N LL P+
Sbjct: 233 HQNIRAVVTGLVHKWAWTKDDVILHVLPLH-----------------HVHGVVNALLCPL 292
Query: 315 YAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQGYEA--MDP 374
+ G+T MP+FS +W+++ S I VF VPT+Y +L++ Y+ P
Sbjct: 293 WVGATCVMMPEFSPQQVWEKF-------LSSETPRINVFMAVPTIYTKLMEYYDRHFTQP 352
Query: 375 ELQ-VASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAISNPL-NG 434
Q A +++RLM+ GS+ALP PV+++W+++TGH LLERYGMTE MA+S PL
Sbjct: 353 HAQDFLRAVCEEKIRLMVSGSAALPLPVLEKWKNITGHTLLERYGMTEIGMALSGPLTTA 412
Query: 435 ERKVGTVGKPFPGIEAKIVAD---------------DE---------SSNEGELCIRSPS 494
R G+VG P PG++ +IV++ DE EGEL +R PS
Sbjct: 413 VRLPGSVGTPLPGVQVRIVSENPQREACSYTIHAEGDERGTKVTPGFEEKEGELLVRGPS 472
Query: 495 MFKEYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIE 554
+F+EYW PE TK +F G+FKTGD ++G Y I GR + DI+K GGYK+SALE+E
Sbjct: 473 VFREYWNKPEETKSAFTLDGWFKTGDTV-VFKDGQYWIRGRTSVDIIKTGGYKVSALEVE 532
Query: 555 SVILQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDK 607
+L HPS+ + V+G+PD +G+RV A++ L +++ EL+ WAR+
Sbjct: 533 WHLLAHPSITDVAVIGVPDMTWGQRVTAVVTLREGH----------SLSHRELKEWARNV 564
BLAST of MS009497.1 vs. ExPASy TrEMBL
Match:
A0A6J1DG96 (malonate--CoA ligase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020569 PE=3 SV=1)
HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 587/606 (96.86%), Postives = 589/606 (97.19%), Query Frame = 0
Query: 1 MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT
Sbjct: 1 MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
Query: 61 FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHG+LGGAK
Sbjct: 61 FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGNLGGAK 120
Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ
Sbjct: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT 240
SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT
Sbjct: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT 240
Query: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVH 300
TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH HVH
Sbjct: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH-----------------HVH 300
Query: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL 360
GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL
Sbjct: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL 360
Query: 361 QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI 420
QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI
Sbjct: 361 QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI 420
Query: 421 SNPLNGERKVGTVGKPFPGIEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLD 480
SNPLNGERKVGTVGKPFPG+EAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLD
Sbjct: 421 SNPLNGERKVGTVGKPFPGVEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLD 480
Query: 481 GGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLP 540
GGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLP
Sbjct: 481 GGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLP 540
Query: 541 DKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPR 600
DKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPR
Sbjct: 541 DKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPR 589
Query: 601 NAMGKV 607
NAMGKV
Sbjct: 601 NAMGKV 589
BLAST of MS009497.1 vs. ExPASy TrEMBL
Match:
A0A6J1DHQ0 (malonate--CoA ligase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111020569 PE=3 SV=1)
HSP 1 Score: 1044.6 bits (2700), Expect = 1.5e-301
Identity = 526/545 (96.51%), Postives = 528/545 (96.88%), Query Frame = 0
Query: 62 MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAKV 121
MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHG+LGGAKV
Sbjct: 1 MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGNLGGAKV 60
Query: 122 GIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQS 181
GIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQS
Sbjct: 61 GIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQS 120
Query: 182 VAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTT 241
VAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTT
Sbjct: 121 VAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTT 180
Query: 242 GKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVHG 301
GKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH HVHG
Sbjct: 181 GKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH-----------------HVHG 240
Query: 302 LFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQ 361
LFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQ
Sbjct: 241 LFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQ 300
Query: 362 GYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAIS 421
GYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAIS
Sbjct: 301 GYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAIS 360
Query: 422 NPLNGERKVGTVGKPFPGIEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLDG 481
NPLNGERKVGTVGKPFPG+EAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLDG
Sbjct: 361 NPLNGERKVGTVGKPFPGVEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLDG 420
Query: 482 GFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPD 541
GFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPD
Sbjct: 421 GFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPD 480
Query: 542 KDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRN 601
KDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRN
Sbjct: 481 KDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRN 528
Query: 602 AMGKV 607
AMGKV
Sbjct: 541 AMGKV 528
BLAST of MS009497.1 vs. ExPASy TrEMBL
Match:
A0A1S3BUN8 (malonate--CoA ligase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493370 PE=3 SV=1)
HSP 1 Score: 976.9 bits (2524), Expect = 4.0e-281
Identity = 498/613 (81.24%), Postives = 535/613 (87.28%), Query Frame = 0
Query: 1 MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
MNSS F +PT F RIC S LI RS S C+ +G+SI K F RSRL+S++SS+T
Sbjct: 1 MNSSPFNNPTSLFTRICHLSLPSSHLIAIRSSSACFCIGKSIPKFCFSRSRLFSVLSSYT 60
Query: 61 FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
FMEVVKS AKHG+T ESVAIRANQKSHSY QLISSALKIS LLTSSD++T HGHLGGAK
Sbjct: 61 FMEVVKSAAKHGATCRESVAIRANQKSHSYIQLISSALKISDLLTSSDLRTGHGHLGGAK 120
Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
+GIVAKPSAEFLAGVLGTW SGGVAVPLALSYPEAELLHVLNDSEVSMILSTED EFMQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180
Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIE---ADADRIFQGTIENLSDDPALIIYT 240
SVAAKT A SLIPPVPHI SERN H+Q T+IE ADADR+FQGT+ENLSDDPALIIYT
Sbjct: 181 SVAAKTVAIVSLIPPVPHIYSERNSHDQSTNIEVNAADADRVFQGTVENLSDDPALIIYT 240
Query: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNL 300
SGTTGKPKGVVHTHKSITSQVQTLS AWEYTSADQFLHCLPLH
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQTLSNAWEYTSADQFLHCLPLH----------------- 300
Query: 301 HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYA 360
HVHG+FNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPS SSKSDDAITVFTGVPTMYA
Sbjct: 301 HVHGMFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYA 360
Query: 361 RLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFV 420
RLLQGYEAMD ELQ+ASASAA+QLRLMMCGSSALPPP+M+QW +VTGH LLERYGMTEFV
Sbjct: 361 RLLQGYEAMDSELQLASASAARQLRLMMCGSSALPPPIMKQWREVTGHELLERYGMTEFV 420
Query: 421 MAISNPLNGERKVGTVGKPFPGIEAKIVADD----ESSNEGELCIRSPSMFKEYWKLPEV 480
MAISNPLNG+RK+GTVGKPFPG+EAKIVADD SS EGELCIRSPSMFKEYWKLP+V
Sbjct: 421 MAISNPLNGQRKIGTVGKPFPGVEAKIVADDGSTTGSSLEGELCIRSPSMFKEYWKLPKV 480
Query: 481 TKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIE 540
TKESF+DGGFFKTGDAAR DE+GYYIILGR++ADIMKVGGYKLSALEIESVILQHPSV E
Sbjct: 481 TKESFIDGGFFKTGDAARLDEDGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVTE 540
Query: 541 CCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLL 600
CCVLGLPDKDYGERVCAI+VL+ N K T PD S P+++L ELRTWA+DKLAPYKLPT LL
Sbjct: 541 CCVLGLPDKDYGERVCAIVVLQANTKTTIPDGSNPSMSLDELRTWAKDKLAPYKLPTMLL 596
Query: 601 LWESLPRNAMGKV 607
L +SLPRNAMGKV
Sbjct: 601 LKDSLPRNAMGKV 596
BLAST of MS009497.1 vs. ExPASy TrEMBL
Match:
A0A6J1KFM3 (malonate--CoA ligase OS=Cucurbita maxima OX=3661 GN=LOC111494602 PE=4 SV=1)
HSP 1 Score: 974.5 bits (2518), Expect = 2.0e-280
Identity = 492/610 (80.66%), Postives = 530/610 (86.89%), Query Frame = 0
Query: 1 MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
MNS TF T+ F RI FAPS LI +S S C L RSI KL FPRSRL+S+ SS T
Sbjct: 1 MNSFTFNYSTYLFSRIYHLSFAPSHLIAIQSCSACIRLHRSIPKLCFPRSRLFSVSSSST 60
Query: 61 FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
FMEVVKS AKHGST E+VAIRAN KSHSY QLISSALKIS LLTS D++T HGHLGGAK
Sbjct: 61 FMEVVKSAAKHGSTCRENVAIRANMKSHSYMQLISSALKISDLLTSCDLRTGHGHLGGAK 120
Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
+GI+AKPSAEFLAG+LGTW SGGVAVPLAL+YPE ELLHVLNDSEVSMILSTED E MQ
Sbjct: 121 IGIIAKPSAEFLAGLLGTWFSGGVAVPLALNYPEPELLHVLNDSEVSMILSTEDHAEIMQ 180
Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT 240
SVAAKTAAKFSLIPPVPHI SE N +Q TDIE DA+R+FQGT+ENLSDDPAL+IYTSGT
Sbjct: 181 SVAAKTAAKFSLIPPVPHIYSETNSRDQSTDIEMDAERVFQGTVENLSDDPALVIYTSGT 240
Query: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVH 300
TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQ LHCLPLH HVH
Sbjct: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQLLHCLPLH-----------------HVH 300
Query: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL 360
GLFNG LAPIYAGSTVEFMPKFSVSGIWKRWRESYPSN SK+DDAIT+FTGVPTMYARLL
Sbjct: 301 GLFNGFLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNGSKNDDAITIFTGVPTMYARLL 360
Query: 361 QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI 420
QGYEAM+P+LQV SASAA+QLRLMMCGSSALP P+M+QW+ VTGH+LLERYGMTEFVMAI
Sbjct: 361 QGYEAMEPDLQVDSASAARQLRLMMCGSSALPTPIMKQWKAVTGHQLLERYGMTEFVMAI 420
Query: 421 SNPLNGERKVGTVGKPFPGIEAKIVADDESSN----EGELCIRSPSMFKEYWKLPEVTKE 480
SNPLNGERK+GTVGKP PG+EAKIV+DD S+N EGELCIRSPSMFKEYWKLP+VTKE
Sbjct: 421 SNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKE 480
Query: 481 SFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCV 540
SF+DGGFFKTGDAAR DE+GYY ILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCV
Sbjct: 481 SFIDGGFFKTGDAARLDEDGYYTILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCV 540
Query: 541 LGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWE 600
LGLPDKDYGERVCAIIVL PN K TPPD SKP ++L +LRTWA+DKLAPYK+PT LLL +
Sbjct: 541 LGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKIPTVLLLKD 593
Query: 601 SLPRNAMGKV 607
SLPRNAMGKV
Sbjct: 601 SLPRNAMGKV 593
BLAST of MS009497.1 vs. ExPASy TrEMBL
Match:
A0A0A0L7U7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G099620 PE=3 SV=1)
HSP 1 Score: 973.0 bits (2514), Expect = 5.7e-280
Identity = 498/614 (81.11%), Postives = 536/614 (87.30%), Query Frame = 0
Query: 1 MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
MN STF +PT F RI SRLI RS S C+ +G+SI K F R RL+S++SS+T
Sbjct: 1 MNYSTFNNPTSLFTRISHLSLPSSRLIAIRSSSACFCIGKSIPKFCFSRFRLFSVLSSYT 60
Query: 61 FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
FMEVVKS AKHG+T E+VAIRANQKSHSY QLISSALKIS LLTSSD++T HGHLGGAK
Sbjct: 61 FMEVVKSAAKHGATCRENVAIRANQKSHSYIQLISSALKISDLLTSSDLRTGHGHLGGAK 120
Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
+GIVAKPSAEFLAGVLGTW SGGVAVPLALSYPEAELLHVLNDSEVSMILSTED EFMQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180
Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIE---ADADRIFQGTIENLSDDPALIIYT 240
SVAAKT A SLIPPVPHI SERN H+Q T+IE ADADR+FQGT+ENLSDDPALIIYT
Sbjct: 181 SVAAKTVATVSLIPPVPHIYSERNSHDQSTNIEVDAADADRVFQGTVENLSDDPALIIYT 240
Query: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNL 300
SGTTGKPKGVVHTHKSITSQVQ LS AWEYTSADQFLHCLPLH
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQMLSNAWEYTSADQFLHCLPLH----------------- 300
Query: 301 HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYA 360
HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPS SSKSDDAITVFTGVPTMYA
Sbjct: 301 HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYA 360
Query: 361 RLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFV 420
RLLQGYEAMD ELQ+ASASAA+QLRLMMCGSSALPPP+M+QW++VTGH LLERYGMTEFV
Sbjct: 361 RLLQGYEAMDSELQLASASAARQLRLMMCGSSALPPPIMKQWKEVTGHELLERYGMTEFV 420
Query: 421 MAISNPLNGERKVGTVGKPFPGIEAKIVADDESSN----EGELCIRSPSMFKEYWKLPEV 480
MAISNPLNG+RK+GTVGKPFPG+EAKIVADD S+N EGELCIRSPSMFKEYWKLPEV
Sbjct: 421 MAISNPLNGQRKIGTVGKPFPGVEAKIVADDGSTNDSSLEGELCIRSPSMFKEYWKLPEV 480
Query: 481 TKESFLDGGFFKTGDAARRDEN-GYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVI 540
TKESF+DGGFFKTGDAAR DE+ GYYIILGR++ADIMKVGGYKLSALEIESVILQHPSVI
Sbjct: 481 TKESFIDGGFFKTGDAARLDEDGGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVI 540
Query: 541 ECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKL 600
ECCVLGLPDKDYGERVCAI+VL+ N K T PD S P+++L ELRTWA+DKLAPYKLPT L
Sbjct: 541 ECCVLGLPDKDYGERVCAIVVLQANMKTTIPDGSNPSMSLDELRTWAKDKLAPYKLPTML 597
Query: 601 LLWESLPRNAMGKV 607
LL +SLPRNAMGKV
Sbjct: 601 LLKDSLPRNAMGKV 597
BLAST of MS009497.1 vs. TAIR 10
Match:
AT3G16170.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 712.2 bits (1837), Expect = 3.5e-205
Identity = 362/555 (65.23%), Postives = 424/555 (76.40%), Query Frame = 0
Query: 62 MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKT---------E 121
MEV K+ S + +AI+A+ KS+SYGQL SSAL+IS L D
Sbjct: 1 MEVFKAAFSEASNSCDRIAIKADGKSYSYGQLTSSALRISKLFLKDDTTNGGQETKKYEG 60
Query: 122 HGHLGGAKVGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILST 181
G L GA++GIVAKPSAEF+AGVLGTW SGGVAVPLALSYPEAELLHV+NDS++S++LST
Sbjct: 61 FGSLKGARIGIVAKPSAEFVAGVLGTWFSGGVAVPLALSYPEAELLHVMNDSDISLLLST 120
Query: 182 EDRGEFMQSVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPA 241
ED E M+++AAK+ A+F LIPPV + +SE Q D +A+ F DDPA
Sbjct: 121 EDHSETMKTIAAKSGARFHLIPPVVNSTSETVACNQFQDDSFEAEGKF-------LDDPA 180
Query: 242 LIIYTSGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVS 301
LI+YTSGTTGKPKGVVHTH SI SQV+ L++AWEYTSAD FLHCLPLH
Sbjct: 181 LIVYTSGTTGKPKGVVHTHNSINSQVRMLTEAWEYTSADHFLHCLPLH------------ 240
Query: 302 PYFNLHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGV 361
HVHGLFN L AP+YA S VEF+PKFSVSGIW+RWRESYP N K++D+ITVFTGV
Sbjct: 241 -----HVHGLFNALFAPLYARSLVEFLPKFSVSGIWRRWRESYPVNDEKTNDSITVFTGV 300
Query: 362 PTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYG 421
PTMY RL+QGYEAMD E+Q +SA AA++LRLMM GSSALP PVM QWE +TGHRLLERYG
Sbjct: 301 PTMYTRLIQGYEAMDKEMQDSSAFAARKLRLMMSGSSALPRPVMHQWESITGHRLLERYG 360
Query: 422 MTEFVMAISNPLNGERKVGTVGKPFPGIEAKIVADDESSN-EGELCIRSPSMFKEYWKLP 481
MTEFVMA+SNPL G R GTVGKP PG+EAKI D+ +N GE+C++SPS+FKEYW LP
Sbjct: 361 MTEFVMAMSNPLRGARNAGTVGKPLPGVEAKIKEDENDANGVGEICVKSPSLFKEYWNLP 420
Query: 482 EVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSV 541
EVTKESF + G+FKTGDA R DE+GYY+ILGR +ADIMKVGGYKLSALEIES +L+HP+V
Sbjct: 421 EVTKESFTEDGYFKTGDAGRVDEDGYYVILGRNSADIMKVGGYKLSALEIESTLLEHPTV 480
Query: 542 IECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTK 601
ECCVLGL D DYGE V AII+ E K+ D+SKP ITL EL WA+DKLAPYKLPT+
Sbjct: 481 AECCVLGLTDNDYGEAVTAIIIAESAAKKRREDESKPVITLEELCGWAKDKLAPYKLPTR 531
Query: 602 LLLWESLPRNAMGKV 607
LL+WESLPRNAMGKV
Sbjct: 541 LLIWESLPRNAMGKV 531
BLAST of MS009497.1 vs. TAIR 10
Match:
AT3G48990.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 200.3 bits (508), Expect = 4.5e-51
Identity = 150/514 (29.18%), Postives = 253/514 (49.22%), Query Frame = 0
Query: 106 SSDIKTEHGHLGGAKVGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSE 165
+S + ++ G G V + + EF+ L + A PL +Y E L+DS+
Sbjct: 44 ASRLVSDAGIKPGDVVALTFPNTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSD 103
Query: 166 VSMILSTEDRGEFMQSVAAKTAAKFSLIPPVPHISSERNPHEQPTDIE-ADADRIFQGTI 225
++L++++ Q A+K + H+++ + AD+D +
Sbjct: 104 SKLLLTSKEGNAPAQEAASKL--------KISHVTATLLDAGSDLVLSVADSDSVVDSAT 163
Query: 226 E--NLSDDPALIIYTSGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHI 285
E N DD AL ++TSGTT +PKGV T ++ S V+ + ++ T +D + LPL
Sbjct: 164 ELVNHPDDGALFLHTSGTTSRPKGVPLTQLNLASSVKNIKAVYKLTESDSTVIVLPL--- 223
Query: 286 LEFSTFVAVSPYFNLHVHGLFNGLLAPIYAGS--TVEFMPKFSVSGIWKRWRESYPSNSS 345
HVHGL GLL+ + AG+ T+ +FS + W ++
Sbjct: 224 --------------FHVHGLLAGLLSSLGAGAAVTLPAAGRFSATTFWPDMKKY------ 283
Query: 346 KSDDAITVFTGVPTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWE 405
T +T VPT++ +L + A PE + +LR + S++L P ++ + E
Sbjct: 284 ----NATWYTAVPTIHQIILDRH-ASHPETEY------PKLRFIRSCSASLAPVILSRLE 343
Query: 406 DVTGHRLLERYGMTEFV-MAISNPL--NGERKVGTVGKPFPGIEAKIVAD----DESSNE 465
+ G +LE Y MTE + SNPL G K G+VGKP G E I+ + E +N+
Sbjct: 344 EAFGAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKPV-GQEMAILNEKGEIQEPNNK 403
Query: 466 GELCIRSPSMFKEYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGG 525
GE+CIR P++ K Y PE K F + G+F TGD D +GY ++GR+ +++ GG
Sbjct: 404 GEVCIRGPNVTKGYKNNPEANKAGF-EFGWFHTGDIGYFDTDGYLHLVGRIK-ELINRGG 463
Query: 526 YKLSALEIESVILQHPSVIECCVLGLPDKDYGERV-CAIIVLEPNFKRTPPDDSKPAITL 585
K+S +E+++V+L HP V + G+PD+ YGE + CA+I E +T
Sbjct: 464 EKISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGT-----------TVTE 501
Query: 586 LELRTWARDKLAPYKLPTKLLLWESLPRNAMGKV 607
+++ + + LA +K+P ++ + ++LP+ A GK+
Sbjct: 524 EDIKAFCKKNLAAFKVPKRVFITDNLPKTASGKI 501
BLAST of MS009497.1 vs. TAIR 10
Match:
AT4G19010.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 142.5 bits (358), Expect = 1.1e-33
Identity = 108/394 (27.41%), Postives = 194/394 (49.24%), Query Frame = 0
Query: 229 DDPALIIYTSGTTGKPKGVVHTHKSITSQVQTL----SKAWEYT-SADQFLHCLPLHHIL 288
DD A I+Y+SGTTG KGV+ TH+++ + ++ + +EY S++ +L LPL HI
Sbjct: 204 DDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLCHIY 263
Query: 289 EFSTFVAVSPYFNLHVHGLFNGLLAPIYAGSTVEFMPKFSVS---GIWKRWRESYPSNSS 348
S FV GLL+ GST+ M +F S + +R++
Sbjct: 264 GLSLFVM--------------GLLS---LGSTIVVMKRFDASDVVNVIERFK-------- 323
Query: 349 KSDDAITVFTGVPTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWE 408
IT F VP M L + + + E+ K L+ + G++ L ++ +
Sbjct: 324 -----ITHFPVVPPMLMALTKKAKGVCGEV-------FKSLKQVSSGAAPLSRKFIEDFL 383
Query: 409 DVTGH-RLLERYGMTEFVMAISNPLNGER--KVGTVGKPFPGIEAKIVADDESS-----N 468
H L++ YGMTE + N E+ + +VG P ++AK+V S N
Sbjct: 384 QTLPHVDLIQGYGMTESTAVGTRGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGN 443
Query: 469 EGELCIRSPSMFKEYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVG 528
GEL I+ P + K Y P+ T+ S ++ + +TGD A DE+GY I+ R+ +I+K
Sbjct: 444 RGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRTGDIAYFDEDGYLFIVDRIK-EIIKYK 503
Query: 529 GYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITL 588
G++++ ++E+V++ HP +I+ V P+++ GE A +V + ++
Sbjct: 504 GFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVAFVVRR----------QETTLSE 549
Query: 589 LELRTWARDKLAPYKLPTKLLLWESLPRNAMGKV 607
++ ++ ++APY+ K+++ S+P++ GK+
Sbjct: 564 EDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKI 549
BLAST of MS009497.1 vs. TAIR 10
Match:
AT1G20510.1 (OPC-8:0 CoA ligase1 )
HSP 1 Score: 142.1 bits (357), Expect = 1.4e-33
Identity = 102/387 (26.36%), Postives = 185/387 (47.80%), Query Frame = 0
Query: 229 DDPALIIYTSGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQ-FLHCLPLHHILEFST 288
DD A ++Y+SGTTG KGV+ +H+++ + VQT+ + +Q F+ +P+ HI +
Sbjct: 189 DDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHIYGLAA 248
Query: 289 FVAVSPYFNLHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAIT 348
F GLLA GST+ + KF E + S+ T
Sbjct: 249 FA--------------TGLLA---YGSTIIVLSKF----------EMHEMMSAIGKYQAT 308
Query: 349 VFTGVPTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQW-EDVTGHR 408
VP + ++ G + + + ++S + ++CG + L V + + E +
Sbjct: 309 SLPLVPPILVAMVNGADQIKAKYDLSS------MHTVLCGGAPLSKEVTEGFAEKYPTVK 368
Query: 409 LLERYGMTEF--VMAISNPLNGERKVGTVGKPFPGIEAKIVADDES-----SNEGELCIR 468
+L+ YG+TE + A ++ + R+ GT GK +E +IV GEL ++
Sbjct: 369 ILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLK 428
Query: 469 SPSMFKEYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSAL 528
PS+ K Y+ E T + G+ +TGD DE+G+ ++ R+ +++K GY+++
Sbjct: 429 GPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLK-ELIKYKGYQVAPA 488
Query: 529 EIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWA 588
E+E+++L HP + + V+ PDK+ G+ A +V + S T++E +
Sbjct: 489 ELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTG-------SSLSEKTIME---FV 531
Query: 589 RDKLAPYKLPTKLLLWESLPRNAMGKV 607
++APYK K+ S+P+N GK+
Sbjct: 549 AKQVAPYKRIRKVAFVSSIPKNPSGKI 531
BLAST of MS009497.1 vs. TAIR 10
Match:
AT1G30520.1 (acyl-activating enzyme 14 )
HSP 1 Score: 133.3 bits (334), Expect = 6.7e-31
Identity = 110/405 (27.16%), Postives = 178/405 (43.95%), Query Frame = 0
Query: 228 SDDPALIIYTSGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFST 287
SDD +I +TSGTTG+PKGV +H + +Q Y D +LH PL HI
Sbjct: 171 SDDAVVICFTSGTTGRPKGVTISHLAFITQSLAKIAIAGYGEDDVYLHTSPLVHI----- 230
Query: 288 FVAVSPYFNLHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAIT 347
G + +A + G+ +PKF + +++ IT
Sbjct: 231 -------------GGLSSAMAMLMVGACHVLLPKFDAKTALQVMEQNH----------IT 290
Query: 348 VFTGVPTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDV-TGHR 407
F VP M A L++ + + + +R ++ G +L ++++ ++ R
Sbjct: 291 CFITVPAMMADLIR------VNRTTKNGAENRGVRKILNGGGSLSSELLKEAVNIFPCAR 350
Query: 408 LLERYGMTEFVMA---------------ISNPLNGERKVGT-VGKPFPGIEAKIVADDES 467
+L YGMTE + ++ PL + K GT VGKP P IE + D++S
Sbjct: 351 ILSAYGMTEACSSLTFMTLHDPTQESFKVTYPLLNQPKQGTCVGKPAPHIELMVKLDEDS 410
Query: 468 SNEGELCIRSPSMFKEYW-----KLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMN 527
S G++ R P YW + T ES + + TGD DE G ++GR N
Sbjct: 411 SRVGKILTRGPHTMLRYWGHQVAQENVETSESRSNEAWLDTGDIGAFDEFGNLWLIGRSN 470
Query: 528 ADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDD 587
I K GG + E+E+V+++HP ++ V+G+ D GE V A + L+ + + ++
Sbjct: 471 GRI-KTGGENVYPEEVEAVLVEHPGIVSAVVIGVIDTRLGEMVVACVRLQEKWIWSDVEN 530
Query: 588 SKPAITLLE--LRTWAR-DKLAPYKLPTKLLLWE-SLPRNAMGKV 607
K + L L+ R L +K+P + + WE P GKV
Sbjct: 531 RKGSFQLSSETLKHHCRTQNLTGFKIPKRFVRWEKQFPLTTTGKV 540
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| M4IS90 | 1.4e-222 | 68.73 | Probable CoA ligase CCL8 OS=Humulus lupulus OX=3486 GN=CCL8 PE=2 SV=1 | [more] |
| Q8H151 | 2.2e-204 | 64.10 | Malonate--CoA ligase OS=Arabidopsis thaliana OX=3702 GN=AAE13 PE=1 SV=2 | [more] |
| Q58DN7 | 1.7e-95 | 36.54 | Malonate--CoA ligase ACSF3, mitochondrial OS=Bos taurus OX=9913 GN=ACSF3 PE=2 SV... | [more] |
| Q3URE1 | 2.7e-93 | 35.48 | Malonate--CoA ligase ACSF3, mitochondrial OS=Mus musculus OX=10090 GN=Acsf3 PE=1... | [more] |
| Q4G176 | 1.0e-92 | 36.17 | Malonate--CoA ligase ACSF3, mitochondrial OS=Homo sapiens OX=9606 GN=ACSF3 PE=1 ... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DG96 | 0.0e+00 | 96.86 | malonate--CoA ligase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020569 P... | [more] |
| A0A6J1DHQ0 | 1.5e-301 | 96.51 | malonate--CoA ligase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111020569 P... | [more] |
| A0A1S3BUN8 | 4.0e-281 | 81.24 | malonate--CoA ligase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493370 PE=3 SV=... | [more] |
| A0A6J1KFM3 | 2.0e-280 | 80.66 | malonate--CoA ligase OS=Cucurbita maxima OX=3661 GN=LOC111494602 PE=4 SV=1 | [more] |
| A0A0A0L7U7 | 5.7e-280 | 81.11 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G099620 PE=3 SV=1 | [more] |
