MS009497 (gene) Bitter gourd (TR) v1

BLAST of MS009497 vs. NCBI nr
Match: XP_022152954.1 (malonate--CoA ligase isoform X1 [Momordica charantia] >XP_022152963.1 malonate--CoA ligase isoform X1 [Momordica charantia])

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 587/606 (96.86%), Postives = 589/606 (97.19%), Query Frame = 0

Query: 1   MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
           MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT
Sbjct: 1   MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60

Query: 61  FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
           FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHG+LGGAK
Sbjct: 61  FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGNLGGAK 120

Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
           VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ
Sbjct: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180

Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT 240
           SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT
Sbjct: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT 240

Query: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVH 300
           TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH                 HVH
Sbjct: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH-----------------HVH 300

Query: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL 360
           GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL
Sbjct: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL 360

Query: 361 QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI 420
           QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI
Sbjct: 361 QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI 420

Query: 421 SNPLNGERKVGTVGKPFPGIEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLD 480
           SNPLNGERKVGTVGKPFPG+EAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLD
Sbjct: 421 SNPLNGERKVGTVGKPFPGVEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLD 480

Query: 481 GGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLP 540
           GGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLP
Sbjct: 481 GGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLP 540

Query: 541 DKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPR 600
           DKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPR
Sbjct: 541 DKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPR 589

Query: 601 NAMGKV 607
           NAMGKV
Sbjct: 601 NAMGKV 589

BLAST of MS009497 vs. NCBI nr
Match: XP_022152972.1 (malonate--CoA ligase isoform X2 [Momordica charantia])

HSP 1 Score: 1044.6 bits (2700), Expect = 3.2e-301
Identity = 526/545 (96.51%), Postives = 528/545 (96.88%), Query Frame = 0

Query: 62  MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAKV 121
           MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHG+LGGAKV
Sbjct: 1   MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGNLGGAKV 60

Query: 122 GIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQS 181
           GIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQS
Sbjct: 61  GIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQS 120

Query: 182 VAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTT 241
           VAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTT
Sbjct: 121 VAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTT 180

Query: 242 GKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVHG 301
           GKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH                 HVHG
Sbjct: 181 GKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH-----------------HVHG 240

Query: 302 LFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQ 361
           LFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQ
Sbjct: 241 LFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQ 300

Query: 362 GYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAIS 421
           GYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAIS
Sbjct: 301 GYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAIS 360

Query: 422 NPLNGERKVGTVGKPFPGIEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLDG 481
           NPLNGERKVGTVGKPFPG+EAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLDG
Sbjct: 361 NPLNGERKVGTVGKPFPGVEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLDG 420

Query: 482 GFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPD 541
           GFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPD
Sbjct: 421 GFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPD 480

Query: 542 KDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRN 601
           KDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRN
Sbjct: 481 KDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRN 528

Query: 602 AMGKV 607
           AMGKV
Sbjct: 541 AMGKV 528

BLAST of MS009497 vs. NCBI nr
Match: XP_038904615.1 (malonate--CoA ligase isoform X1 [Benincasa hispida])

HSP 1 Score: 984.9 bits (2545), Expect = 3.0e-283
Identity = 500/613 (81.57%), Postives = 538/613 (87.77%), Query Frame = 0

Query: 1   MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
           MNSSTF + T  F RIC     PS LI  RS S C++LGR+  +L F R RL+S++SS+T
Sbjct: 1   MNSSTFNNLTSLFTRICHLSLPPSHLIAIRSTSACFHLGRNTPQLCFSRFRLFSVLSSYT 60

Query: 61  FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
           FMEVVKS AKHGST  E+VAIRANQKSHSY QLISSALKIS LLTSS+++T H HLGGAK
Sbjct: 61  FMEVVKSAAKHGSTCRENVAIRANQKSHSYIQLISSALKISDLLTSSNLRTGHAHLGGAK 120

Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
           +GIVAKPSAEFLAGVLGTW SGGVAVPLALSYPEAELLHVLNDSEVSMILSTED  EFMQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180

Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIE---ADADRIFQGTIENLSDDPALIIYT 240
           SVAAKT A  SLIPPVPHI SERN H+Q TDIE   ADADR+FQGT+ENLSDDPALIIYT
Sbjct: 181 SVAAKTVATVSLIPPVPHIYSERNSHDQSTDIEVDAADADRVFQGTVENLSDDPALIIYT 240

Query: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNL 300
           SGTTGKPKGVVHTHKSITSQV TLS AWEYTSADQFLHCLPLH                 
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVHTLSNAWEYTSADQFLHCLPLH----------------- 300

Query: 301 HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYA 360
           HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPS SSKSDDAITVFTGVPTMYA
Sbjct: 301 HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYA 360

Query: 361 RLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFV 420
           RLLQGYEAMD ELQ+ASASAA+QLRLMMCGSSALPPP+M+QW++VTGH LLERYGMTEFV
Sbjct: 361 RLLQGYEAMDSELQLASASAARQLRLMMCGSSALPPPIMKQWKEVTGHELLERYGMTEFV 420

Query: 421 MAISNPLNGERKVGTVGKPFPGIEAKIVADDESSN----EGELCIRSPSMFKEYWKLPEV 480
           MAISNPLNG+RK+GTVGKPFPG+EAKIVADD S+N    EGELCIRSPSMFKEYWKLPEV
Sbjct: 421 MAISNPLNGQRKIGTVGKPFPGVEAKIVADDGSTNDSSLEGELCIRSPSMFKEYWKLPEV 480

Query: 481 TKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIE 540
           TKESF+DGGFFKTGDAAR DE+GYYIILGR++ADIMKVGGYKLSALEIESVILQHPSV+E
Sbjct: 481 TKESFIDGGFFKTGDAARLDEDGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVVE 540

Query: 541 CCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLL 600
           CCVLGLPDKDYGERVCAIIVL+PN K T PD+S P ++L ELRTWA+DKLAPYKLPT LL
Sbjct: 541 CCVLGLPDKDYGERVCAIIVLQPNTKTTTPDESNPTMSLDELRTWAKDKLAPYKLPTMLL 596

Query: 601 LWESLPRNAMGKV 607
           L +SLPRNAMGKV
Sbjct: 601 LKDSLPRNAMGKV 596

BLAST of MS009497 vs. NCBI nr
Match: XP_023515293.1 (malonate--CoA ligase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 981.1 bits (2535), Expect = 4.3e-282
Identity = 493/610 (80.82%), Postives = 533/610 (87.38%), Query Frame = 0

Query: 1   MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
           MNS TF   T+ F RIC   FAPS LI  +S S C  L RSI K+ FPRSRL+S+ SS T
Sbjct: 1   MNSFTFNYSTYLFRRICHLSFAPSHLIAIQSCSACLRLHRSIPKICFPRSRLFSVSSSST 60

Query: 61  FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
           FMEVVKS AKHGST  E+VAIRAN KSHSY QLISSALKIS L TS D++T HGHL GAK
Sbjct: 61  FMEVVKSAAKHGSTCRENVAIRANMKSHSYMQLISSALKISDLFTSCDLRTGHGHLDGAK 120

Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
           +GI+AKPSAEFLAG+LGTW SGGVAVPLAL+YPE ELLHVLNDSEVSMILSTED  E MQ
Sbjct: 121 IGIIAKPSAEFLAGLLGTWFSGGVAVPLALNYPEPELLHVLNDSEVSMILSTEDHAEIMQ 180

Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT 240
           SVAAKTAAKFSLIPPVPHI SE N  +Q TDIE DA+R+FQGT+ENLSDDPAL+IYTSGT
Sbjct: 181 SVAAKTAAKFSLIPPVPHIYSETNSRDQSTDIEMDAERVFQGTVENLSDDPALVIYTSGT 240

Query: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVH 300
           TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQ LHCLPLH                 HVH
Sbjct: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQLLHCLPLH-----------------HVH 300

Query: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL 360
           GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSN SK+DDAIT+FTGVPTMYARLL
Sbjct: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNGSKNDDAITIFTGVPTMYARLL 360

Query: 361 QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI 420
           QGYEAM+P+LQVASA+AA+QLRLMMCGSSALPPP+M+QW+ VTGH+LLERYGMTEFVMAI
Sbjct: 361 QGYEAMEPDLQVASANAARQLRLMMCGSSALPPPIMKQWKAVTGHQLLERYGMTEFVMAI 420

Query: 421 SNPLNGERKVGTVGKPFPGIEAKIVADDESSN----EGELCIRSPSMFKEYWKLPEVTKE 480
           SNPLNGERK+GTVGKP PG+EAKIV+DD S+N    EGELCIRSPSMFKEYWKLP+VTKE
Sbjct: 421 SNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKE 480

Query: 481 SFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCV 540
           SF+DGGFFKTGDAAR DE+GYY ILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCV
Sbjct: 481 SFIDGGFFKTGDAARLDEDGYYTILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCV 540

Query: 541 LGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWE 600
           LGLPDKDYGERVCAIIVL PN K TPPD SKP ++L +LRTWA+DKLAPYKLPT+LLL +
Sbjct: 541 LGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKLPTELLLKD 593

Query: 601 SLPRNAMGKV 607
           SLPRNAMGKV
Sbjct: 601 SLPRNAMGKV 593

BLAST of MS009497 vs. NCBI nr
Match: XP_008452304.1 (PREDICTED: malonate--CoA ligase isoform X1 [Cucumis melo])

HSP 1 Score: 976.9 bits (2524), Expect = 8.2e-281
Identity = 498/613 (81.24%), Postives = 535/613 (87.28%), Query Frame = 0

Query: 1   MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
           MNSS F +PT  F RIC      S LI  RS S C+ +G+SI K  F RSRL+S++SS+T
Sbjct: 1   MNSSPFNNPTSLFTRICHLSLPSSHLIAIRSSSACFCIGKSIPKFCFSRSRLFSVLSSYT 60

Query: 61  FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
           FMEVVKS AKHG+T  ESVAIRANQKSHSY QLISSALKIS LLTSSD++T HGHLGGAK
Sbjct: 61  FMEVVKSAAKHGATCRESVAIRANQKSHSYIQLISSALKISDLLTSSDLRTGHGHLGGAK 120

Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
           +GIVAKPSAEFLAGVLGTW SGGVAVPLALSYPEAELLHVLNDSEVSMILSTED  EFMQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180

Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIE---ADADRIFQGTIENLSDDPALIIYT 240
           SVAAKT A  SLIPPVPHI SERN H+Q T+IE   ADADR+FQGT+ENLSDDPALIIYT
Sbjct: 181 SVAAKTVAIVSLIPPVPHIYSERNSHDQSTNIEVNAADADRVFQGTVENLSDDPALIIYT 240

Query: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNL 300
           SGTTGKPKGVVHTHKSITSQVQTLS AWEYTSADQFLHCLPLH                 
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQTLSNAWEYTSADQFLHCLPLH----------------- 300

Query: 301 HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYA 360
           HVHG+FNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPS SSKSDDAITVFTGVPTMYA
Sbjct: 301 HVHGMFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYA 360

Query: 361 RLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFV 420
           RLLQGYEAMD ELQ+ASASAA+QLRLMMCGSSALPPP+M+QW +VTGH LLERYGMTEFV
Sbjct: 361 RLLQGYEAMDSELQLASASAARQLRLMMCGSSALPPPIMKQWREVTGHELLERYGMTEFV 420

Query: 421 MAISNPLNGERKVGTVGKPFPGIEAKIVADD----ESSNEGELCIRSPSMFKEYWKLPEV 480
           MAISNPLNG+RK+GTVGKPFPG+EAKIVADD     SS EGELCIRSPSMFKEYWKLP+V
Sbjct: 421 MAISNPLNGQRKIGTVGKPFPGVEAKIVADDGSTTGSSLEGELCIRSPSMFKEYWKLPKV 480

Query: 481 TKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIE 540
           TKESF+DGGFFKTGDAAR DE+GYYIILGR++ADIMKVGGYKLSALEIESVILQHPSV E
Sbjct: 481 TKESFIDGGFFKTGDAARLDEDGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVTE 540

Query: 541 CCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLL 600
           CCVLGLPDKDYGERVCAI+VL+ N K T PD S P+++L ELRTWA+DKLAPYKLPT LL
Sbjct: 541 CCVLGLPDKDYGERVCAIVVLQANTKTTIPDGSNPSMSLDELRTWAKDKLAPYKLPTMLL 596

Query: 601 LWESLPRNAMGKV 607
           L +SLPRNAMGKV
Sbjct: 601 LKDSLPRNAMGKV 596

BLAST of MS009497 vs. ExPASy Swiss-Prot
Match: M4IS90 (Probable CoA ligase CCL8 OS=Humulus lupulus OX=3486 GN=CCL8 PE=2 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 1.4e-222
Identity = 400/582 (68.73%), Postives = 459/582 (78.87%), Query Frame = 0

Query: 48  PRSRLYSIISSHTFMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSS 107
           P S L+S  ++   ME++K  AK GS+ S  VAIRA+QKS+SY QLISSA +I +LL S+
Sbjct: 31  PFSSLHSGSANLILMELLKEVAKEGSSASVGVAIRADQKSYSYKQLISSAQRICSLLCST 90

Query: 108 DIKTE-----------------HGHLGGAKVGIVAKPSAEFLAGVLGTWLSGGVAVPLAL 167
           D+K                   HGHLGGA+VGIVAKPSAEF+ GVLGTWLSGGVAVPLAL
Sbjct: 91  DLKASHKAGKQVTPALINGLSGHGHLGGARVGIVAKPSAEFVTGVLGTWLSGGVAVPLAL 150

Query: 168 SYPEAELLHVLNDSEVSMILSTEDRGEFMQSVAAKTAAKFSLIPPVPHISSERN--PHEQ 227
           SYPEAELLHV+ DS++SMILSTED  E MQ +AAKTAA+FSLIPPVP   S+     H Q
Sbjct: 151 SYPEAELLHVMTDSDISMILSTEDHQELMQKIAAKTAAQFSLIPPVPSSCSQEGAVDHLQ 210

Query: 228 PTDIEADADRIFQGTIENLSDDPALIIYTSGTTGKPKGVVHTHKSITSQVQTLSKAWEYT 287
             DI  D+  I   T E  +++PALI+YTSGTTGKPKGVVHTHKSI +QVQTL+KAWEYT
Sbjct: 211 TGDINTDS--ILHNT-EISNENPALIVYTSGTTGKPKGVVHTHKSINAQVQTLAKAWEYT 270

Query: 288 SADQFLHCLPLHHILEFSTFVAVSPYFNLHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIW 347
            ADQFLHCLPLH                 HVHGLFN L AP+YA STVEFMPKFSV GIW
Sbjct: 271 PADQFLHCLPLH-----------------HVHGLFNALFAPLYARSTVEFMPKFSVRGIW 330

Query: 348 KRWRESYPSNSSKSDDAITVFTGVPTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGS 407
           +RWRESYP+  +K DD ITVFTGVPTMY RL+QGYEAMDPEL+ ASASAAKQLRLMMCGS
Sbjct: 331 QRWRESYPTCETKVDDCITVFTGVPTMYTRLIQGYEAMDPELKEASASAAKQLRLMMCGS 390

Query: 408 SALPPPVMQQWEDVTGHRLLERYGMTEFVMAISNPLNGERKVGTVGKPFPGIEAKIVADD 467
           SALP PVMQQW+ +TGHRLLERYGMTEFVMAISNPL GERK GTVGKPFPG+E +IVA+D
Sbjct: 391 SALPIPVMQQWQTITGHRLLERYGMTEFVMAISNPLKGERKAGTVGKPFPGVEVRIVAED 450

Query: 468 ESSNE----GELCIRSPSMFKEYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRM 527
           E+ ++    GELC+RSPS+FKEYW+LPEVTK SF D GFFKTGDA + DE+GYY+ILGR 
Sbjct: 451 ENGSDTTGVGELCVRSPSLFKEYWRLPEVTKSSFTDDGFFKTGDAGKVDEDGYYVILGRT 510

Query: 528 NADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPD 587
           +ADIMKVGGYKLSALEIESV+L+HP+V ECCVLGLPDKDYGE V AIIV     K+   +
Sbjct: 511 SADIMKVGGYKLSALEIESVLLEHPTVEECCVLGLPDKDYGEAVSAIIVPAAEAKKKGEE 570

Query: 588 DSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRNAMGKV 607
           +SKPAI+L EL +WA+ KLAPYKLPT+L LW+SLPRNAMGKV
Sbjct: 571 ESKPAISLEELFSWAQHKLAPYKLPTRLFLWDSLPRNAMGKV 592

BLAST of MS009497 vs. ExPASy Swiss-Prot
Match: Q8H151 (Malonate--CoA ligase OS=Arabidopsis thaliana OX=3702 GN=AAE13 PE=1 SV=2)

HSP 1 Score: 713.4 bits (1840), Expect = 2.2e-204
Identity = 366/571 (64.10%), Postives = 430/571 (75.31%), Query Frame = 0

Query: 46  RFPRSRLYSIISSHTFMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLT 105
           RF +S       S + MEV K+     S   + +AI+A+ KS+SYGQL SSAL+IS L  
Sbjct: 49  RFFQSNHLFSSQSGSLMEVFKAAFSEASNSCDRIAIKADGKSYSYGQLTSSALRISKLFL 108

Query: 106 SSDIKT---------EHGHLGGAKVGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAE 165
             D              G L GA++GIVAKPSAEF+AGVLGTW SGGVAVPLALSYPEAE
Sbjct: 109 KDDTTNGGQETKKYEGFGSLKGARIGIVAKPSAEFVAGVLGTWFSGGVAVPLALSYPEAE 168

Query: 166 LLHVLNDSEVSMILSTEDRGEFMQSVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADA 225
           LLHV+NDS++S++LSTED  E M+++AAK+ A+F LIPPV + +SE     Q  D   +A
Sbjct: 169 LLHVMNDSDISLLLSTEDHSETMKTIAAKSGARFHLIPPVVNSTSETVACNQFQDDSFEA 228

Query: 226 DRIFQGTIENLSDDPALIIYTSGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHC 285
           +  F        DDPALI+YTSGTTGKPKGVVHTH SI SQV+ L++AWEYTSAD FLHC
Sbjct: 229 EGKF-------LDDPALIVYTSGTTGKPKGVVHTHNSINSQVRMLTEAWEYTSADHFLHC 288

Query: 286 LPLHHILEFSTFVAVSPYFNLHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYP 345
           LPLH                 HVHGLFN L AP+YA S VEF+PKFSVSGIW+RWRESYP
Sbjct: 289 LPLH-----------------HVHGLFNALFAPLYARSLVEFLPKFSVSGIWRRWRESYP 348

Query: 346 SNSSKSDDAITVFTGVPTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVM 405
            N  K++D+ITVFTGVPTMY RL+QGYEAMD E+Q +SA AA++LRLMM GSSALP PVM
Sbjct: 349 VNDEKTNDSITVFTGVPTMYTRLIQGYEAMDKEMQDSSAFAARKLRLMMSGSSALPRPVM 408

Query: 406 QQWEDVTGHRLLERYGMTEFVMAISNPLNGERKVGTVGKPFPGIEAKIVADDESSN-EGE 465
            QWE +TGHRLLERYGMTEFVMA+SNPL G R  GTVGKP PG+EAKI  D+  +N  GE
Sbjct: 409 HQWESITGHRLLERYGMTEFVMAMSNPLRGARNAGTVGKPLPGVEAKIKEDENDANGVGE 468

Query: 466 LCIRSPSMFKEYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYK 525
           +C++SPS+FKEYW LPEVTKESF + G+FKTGDA R DE+GYY+ILGR +ADIMKVGGYK
Sbjct: 469 ICVKSPSLFKEYWNLPEVTKESFTEDGYFKTGDAGRVDEDGYYVILGRNSADIMKVGGYK 528

Query: 526 LSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLEL 585
           LSALEIES +L+HP+V ECCVLGL D DYGE V AII+ E   K+   D+SKP ITL EL
Sbjct: 529 LSALEIESTLLEHPTVAECCVLGLTDNDYGEAVTAIIIAESAAKKRREDESKPVITLEEL 588

Query: 586 RTWARDKLAPYKLPTKLLLWESLPRNAMGKV 607
             WA+DKLAPYKLPT+LL+WESLPRNAMGKV
Sbjct: 589 CGWAKDKLAPYKLPTRLLIWESLPRNAMGKV 595

BLAST of MS009497 vs. ExPASy Swiss-Prot
Match: Q58DN7 (Malonate--CoA ligase ACSF3, mitochondrial OS=Bos taurus OX=9913 GN=ACSF3 PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.7e-95
Identity = 205/561 (36.54%), Postives = 302/561 (53.83%), Query Frame = 0

Query: 75  FSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAKVGIVAKPSAEFLAG 134
           F + +A+      H+Y  L   +L++S  +       + G L   +V ++      F+  
Sbjct: 54  FGDRLALIDQHGRHTYKDLYLRSLRLSREICQLRACAD-GDLREERVSLLCSNDVSFVVA 113

Query: 135 VLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQSVAAKTAAKFSLIP 194
               W+SGGVAVPL   +P A+L + + DS  S++L+  +  E +  VA K       +P
Sbjct: 114 QWAAWMSGGVAVPLYRKHPRAQLEYFIQDSRSSVVLAGPEHVELLSPVAQKLGVPLLPLP 173

Query: 195 PVPHISSERNPHEQPTDIEADADRIFQGTI--ENLSDDPALIIYTSGTTGKPKGVVHTHK 254
           P  +     +P E              G +   N  D  A+IIYTSGTTG+PKGV+ TH 
Sbjct: 174 PTVYHGVAEDPEE--------------GLVLERNWRDRGAMIIYTSGTTGRPKGVLSTHD 233

Query: 255 SITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVHGLFNGLLAPIYA 314
           +I + V  L   W +T  D  LH LPLH                 HVHG+ N LL P++ 
Sbjct: 234 NIRAVVTGLVHKWAWTKDDVILHVLPLH-----------------HVHGVVNKLLCPLWV 293

Query: 315 GSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQGYEA--MDPEL 374
           G+T   +P+FS   +W+++        S     I VF  VPT+Y++L+  Y+     P +
Sbjct: 294 GATCVMLPEFSAQLVWEKF-------LSSEAPQINVFMAVPTIYSKLMDYYDKHFTQPHV 353

Query: 375 Q-VASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAISNPLNGERK 434
           Q    A   +++RLM+ GS+ALP PV+++W+ +TGH LLERYGMTE  MA+SNPL   R 
Sbjct: 354 QDFVRAVCEEKIRLMVSGSAALPLPVLEKWKGITGHTLLERYGMTEIGMALSNPLTAARL 413

Query: 435 VGTVGKPFPGIEAKIVADDESSN------------------------EGELCIRSPSMFK 494
            G+VG P PG+E +IV+++   +                        EGEL +R PS+F+
Sbjct: 414 PGSVGTPLPGVEVRIVSENPQKDSSPYLIHAEGSEENTKVTPGFEEKEGELLVRGPSVFR 473

Query: 495 EYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVI 554
           EYW  PE TK +F   G+FKTGD     ++G Y I GR + DI+K GGYK+SALE+E ++
Sbjct: 474 EYWDKPEETKAAFTSDGWFKTGDTV-VFKDGCYWIRGRTSVDIIKSGGYKVSALEVERLL 533

Query: 555 LQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAP 607
           L HPS+ +  V+G+PD  +G+RV A++ L+             +++  EL+ WAR  LAP
Sbjct: 534 LAHPSITDVAVIGVPDMTWGQRVTAVVTLQEGH----------SLSHRELKEWARGVLAP 564

BLAST of MS009497 vs. ExPASy Swiss-Prot
Match: Q3URE1 (Malonate--CoA ligase ACSF3, mitochondrial OS=Mus musculus OX=10090 GN=Acsf3 PE=1 SV=2)

HSP 1 Score: 344.4 bits (882), Expect = 2.7e-93
Identity = 198/558 (35.48%), Postives = 298/558 (53.41%), Query Frame = 0

Query: 75  FSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAKVGIVAKPSAEFLAG 134
           F + +A+      H+Y +L   +L ++  +       + G L   +V  +      ++  
Sbjct: 53  FGDRIALIDKYGHHTYRELYDRSLCLAQEICRLQ-GCKVGDLQEERVSFLCSNDVSYVVA 112

Query: 135 VLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQSVAAKTAAKFSLIP 194
              +W+SGGVAVPL   +PEA+L + + DS  S+++  ++  E +  +A +       + 
Sbjct: 113 QWASWMSGGVAVPLYWKHPEAQLEYFIQDSRSSLVVVGQEYLERLSPLAQRLGVPLLPLT 172

Query: 195 PVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTTGKPKGVVHTHKSI 254
           P  +  +   P EQP +     DR             A+I YTSGTTG+PKG + TH+++
Sbjct: 173 PAVYHGATEKPTEQPVEESGWRDR------------GAMIFYTSGTTGRPKGALSTHRNL 232

Query: 255 TSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVHGLFNGLLAPIYAGS 314
            + V  L  +W +T  D  LH LPLH                 HVHG+ N LL P++ G+
Sbjct: 233 AAVVTGLVHSWAWTKNDVILHVLPLH-----------------HVHGVVNKLLCPLWVGA 292

Query: 315 TVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQGYEA--MDPELQ- 374
           T   +P+FS   +W+++        S     ITVF  VPT+Y++LL  Y+     P +Q 
Sbjct: 293 TCVMLPEFSAQQVWEKF-------LSSEAPQITVFMAVPTVYSKLLDYYDKHFTQPHVQD 352

Query: 375 VASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAISNPLNGERKVG 434
              A   +++RLM+ GS+ALP P++++W   TGH LLERYGMTE  MA+SNPL   R  G
Sbjct: 353 FVRAVCKERIRLMVSGSAALPVPLLEKWRSATGHTLLERYGMTEIGMALSNPLTEARVPG 412

Query: 435 TVGKPFPGIEAKIVADDE-----------------------SSNEGELCIRSPSMFKEYW 494
           +VG P PG+E +I++++                           EGEL +R PS+F+EYW
Sbjct: 413 SVGTPLPGVEVRIISENPQKGSPYIIHAEGNERGTKVTPGFEEKEGELLVRGPSVFREYW 472

Query: 495 KLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQH 554
             PE TK +F   G+F+TGD A   +  Y+ I GR + DI+K GGYK+SALEIE  +L H
Sbjct: 473 DKPEETKSAFTSDGWFRTGDTAVFKDARYW-IRGRTSVDIIKTGGYKVSALEIERHLLAH 532

Query: 555 PSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKL 607
           PS+ +  V+G+PD  +G+RV A++ L+     +  D          L+ WAR  LAPY +
Sbjct: 533 PSITDVAVIGVPDMTWGQRVTAVVALQEGHSLSHGD----------LKEWARGVLAPYAV 562

BLAST of MS009497 vs. ExPASy Swiss-Prot
Match: Q4G176 (Malonate--CoA ligase ACSF3, mitochondrial OS=Homo sapiens OX=9606 GN=ACSF3 PE=1 SV=3)

HSP 1 Score: 342.4 bits (877), Expect = 1.0e-92
Identity = 204/564 (36.17%), Postives = 308/564 (54.61%), Query Frame = 0

Query: 75  FSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGG----AKVGIVAKPSAE 134
           F + +A+      H+Y +L S +L++     S +I    G +GG     +V  +    A 
Sbjct: 53  FGDRIALVDQHGRHTYRELYSRSLRL-----SQEICRLCGCVGGDLREERVSFLCANDAS 112

Query: 135 FLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQSVAAKTAAKF 194
           ++     +W+SGGVAVPL   +P A+L +V+ DS+ S++L++++  E +  V  K     
Sbjct: 113 YVVAQWASWMSGGVAVPLYRKHPAAQLEYVICDSQSSVVLASQEYLELLSPVVRKLGVPL 172

Query: 195 SLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTTGKPKGVVHT 254
             + P  +  +   P E P   +   ++             A+IIYTSGTTG+PKGV+ T
Sbjct: 173 LPLTPAIYTGAVEEPAEVPVPEQGWRNK------------GAMIIYTSGTTGRPKGVLST 232

Query: 255 HKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVHGLFNGLLAPI 314
           H++I + V  L   W +T  D  LH LPLH                 HVHG+ N LL P+
Sbjct: 233 HQNIRAVVTGLVHKWAWTKDDVILHVLPLH-----------------HVHGVVNALLCPL 292

Query: 315 YAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQGYEA--MDP 374
           + G+T   MP+FS   +W+++        S     I VF  VPT+Y +L++ Y+     P
Sbjct: 293 WVGATCVMMPEFSPQQVWEKF-------LSSETPRINVFMAVPTIYTKLMEYYDRHFTQP 352

Query: 375 ELQ-VASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAISNPL-NG 434
             Q    A   +++RLM+ GS+ALP PV+++W+++TGH LLERYGMTE  MA+S PL   
Sbjct: 353 HAQDFLRAVCEEKIRLMVSGSAALPLPVLEKWKNITGHTLLERYGMTEIGMALSGPLTTA 412

Query: 435 ERKVGTVGKPFPGIEAKIVAD---------------DE---------SSNEGELCIRSPS 494
            R  G+VG P PG++ +IV++               DE            EGEL +R PS
Sbjct: 413 VRLPGSVGTPLPGVQVRIVSENPQREACSYTIHAEGDERGTKVTPGFEEKEGELLVRGPS 472

Query: 495 MFKEYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIE 554
           +F+EYW  PE TK +F   G+FKTGD     ++G Y I GR + DI+K GGYK+SALE+E
Sbjct: 473 VFREYWNKPEETKSAFTLDGWFKTGDTV-VFKDGQYWIRGRTSVDIIKTGGYKVSALEVE 532

Query: 555 SVILQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDK 607
             +L HPS+ +  V+G+PD  +G+RV A++ L              +++  EL+ WAR+ 
Sbjct: 533 WHLLAHPSITDVAVIGVPDMTWGQRVTAVVTLREGH----------SLSHRELKEWARNV 564

BLAST of MS009497 vs. ExPASy TrEMBL
Match: A0A6J1DG96 (malonate--CoA ligase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020569 PE=3 SV=1)

HSP 1 Score: 1165.2 bits (3013), Expect = 0.0e+00
Identity = 587/606 (96.86%), Postives = 589/606 (97.19%), Query Frame = 0

Query: 1   MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
           MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT
Sbjct: 1   MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60

Query: 61  FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
           FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHG+LGGAK
Sbjct: 61  FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGNLGGAK 120

Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
           VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ
Sbjct: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180

Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT 240
           SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT
Sbjct: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT 240

Query: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVH 300
           TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH                 HVH
Sbjct: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH-----------------HVH 300

Query: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL 360
           GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL
Sbjct: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL 360

Query: 361 QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI 420
           QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI
Sbjct: 361 QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI 420

Query: 421 SNPLNGERKVGTVGKPFPGIEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLD 480
           SNPLNGERKVGTVGKPFPG+EAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLD
Sbjct: 421 SNPLNGERKVGTVGKPFPGVEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLD 480

Query: 481 GGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLP 540
           GGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLP
Sbjct: 481 GGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLP 540

Query: 541 DKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPR 600
           DKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPR
Sbjct: 541 DKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPR 589

Query: 601 NAMGKV 607
           NAMGKV
Sbjct: 601 NAMGKV 589

BLAST of MS009497 vs. ExPASy TrEMBL
Match: A0A6J1DHQ0 (malonate--CoA ligase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111020569 PE=3 SV=1)

HSP 1 Score: 1044.6 bits (2700), Expect = 1.5e-301
Identity = 526/545 (96.51%), Postives = 528/545 (96.88%), Query Frame = 0

Query: 62  MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAKV 121
           MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHG+LGGAKV
Sbjct: 1   MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGNLGGAKV 60

Query: 122 GIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQS 181
           GIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQS
Sbjct: 61  GIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQS 120

Query: 182 VAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTT 241
           VAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTT
Sbjct: 121 VAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGTT 180

Query: 242 GKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVHG 301
           GKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH                 HVHG
Sbjct: 181 GKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLH-----------------HVHG 240

Query: 302 LFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQ 361
           LFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQ
Sbjct: 241 LFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLLQ 300

Query: 362 GYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAIS 421
           GYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAIS
Sbjct: 301 GYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAIS 360

Query: 422 NPLNGERKVGTVGKPFPGIEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLDG 481
           NPLNGERKVGTVGKPFPG+EAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLDG
Sbjct: 361 NPLNGERKVGTVGKPFPGVEAKIVADDESSNEGELCIRSPSMFKEYWKLPEVTKESFLDG 420

Query: 482 GFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPD 541
           GFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPD
Sbjct: 421 GFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPD 480

Query: 542 KDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRN 601
           KDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRN
Sbjct: 481 KDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWESLPRN 528

Query: 602 AMGKV 607
           AMGKV
Sbjct: 541 AMGKV 528

BLAST of MS009497 vs. ExPASy TrEMBL
Match: A0A1S3BUN8 (malonate--CoA ligase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493370 PE=3 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 4.0e-281
Identity = 498/613 (81.24%), Postives = 535/613 (87.28%), Query Frame = 0

Query: 1   MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
           MNSS F +PT  F RIC      S LI  RS S C+ +G+SI K  F RSRL+S++SS+T
Sbjct: 1   MNSSPFNNPTSLFTRICHLSLPSSHLIAIRSSSACFCIGKSIPKFCFSRSRLFSVLSSYT 60

Query: 61  FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
           FMEVVKS AKHG+T  ESVAIRANQKSHSY QLISSALKIS LLTSSD++T HGHLGGAK
Sbjct: 61  FMEVVKSAAKHGATCRESVAIRANQKSHSYIQLISSALKISDLLTSSDLRTGHGHLGGAK 120

Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
           +GIVAKPSAEFLAGVLGTW SGGVAVPLALSYPEAELLHVLNDSEVSMILSTED  EFMQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180

Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIE---ADADRIFQGTIENLSDDPALIIYT 240
           SVAAKT A  SLIPPVPHI SERN H+Q T+IE   ADADR+FQGT+ENLSDDPALIIYT
Sbjct: 181 SVAAKTVAIVSLIPPVPHIYSERNSHDQSTNIEVNAADADRVFQGTVENLSDDPALIIYT 240

Query: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNL 300
           SGTTGKPKGVVHTHKSITSQVQTLS AWEYTSADQFLHCLPLH                 
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQTLSNAWEYTSADQFLHCLPLH----------------- 300

Query: 301 HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYA 360
           HVHG+FNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPS SSKSDDAITVFTGVPTMYA
Sbjct: 301 HVHGMFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYA 360

Query: 361 RLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFV 420
           RLLQGYEAMD ELQ+ASASAA+QLRLMMCGSSALPPP+M+QW +VTGH LLERYGMTEFV
Sbjct: 361 RLLQGYEAMDSELQLASASAARQLRLMMCGSSALPPPIMKQWREVTGHELLERYGMTEFV 420

Query: 421 MAISNPLNGERKVGTVGKPFPGIEAKIVADD----ESSNEGELCIRSPSMFKEYWKLPEV 480
           MAISNPLNG+RK+GTVGKPFPG+EAKIVADD     SS EGELCIRSPSMFKEYWKLP+V
Sbjct: 421 MAISNPLNGQRKIGTVGKPFPGVEAKIVADDGSTTGSSLEGELCIRSPSMFKEYWKLPKV 480

Query: 481 TKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIE 540
           TKESF+DGGFFKTGDAAR DE+GYYIILGR++ADIMKVGGYKLSALEIESVILQHPSV E
Sbjct: 481 TKESFIDGGFFKTGDAARLDEDGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVTE 540

Query: 541 CCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLL 600
           CCVLGLPDKDYGERVCAI+VL+ N K T PD S P+++L ELRTWA+DKLAPYKLPT LL
Sbjct: 541 CCVLGLPDKDYGERVCAIVVLQANTKTTIPDGSNPSMSLDELRTWAKDKLAPYKLPTMLL 596

Query: 601 LWESLPRNAMGKV 607
           L +SLPRNAMGKV
Sbjct: 601 LKDSLPRNAMGKV 596

BLAST of MS009497 vs. ExPASy TrEMBL
Match: A0A6J1KFM3 (malonate--CoA ligase OS=Cucurbita maxima OX=3661 GN=LOC111494602 PE=4 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 2.0e-280
Identity = 492/610 (80.66%), Postives = 530/610 (86.89%), Query Frame = 0

Query: 1   MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
           MNS TF   T+ F RI    FAPS LI  +S S C  L RSI KL FPRSRL+S+ SS T
Sbjct: 1   MNSFTFNYSTYLFSRIYHLSFAPSHLIAIQSCSACIRLHRSIPKLCFPRSRLFSVSSSST 60

Query: 61  FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
           FMEVVKS AKHGST  E+VAIRAN KSHSY QLISSALKIS LLTS D++T HGHLGGAK
Sbjct: 61  FMEVVKSAAKHGSTCRENVAIRANMKSHSYMQLISSALKISDLLTSCDLRTGHGHLGGAK 120

Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
           +GI+AKPSAEFLAG+LGTW SGGVAVPLAL+YPE ELLHVLNDSEVSMILSTED  E MQ
Sbjct: 121 IGIIAKPSAEFLAGLLGTWFSGGVAVPLALNYPEPELLHVLNDSEVSMILSTEDHAEIMQ 180

Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPALIIYTSGT 240
           SVAAKTAAKFSLIPPVPHI SE N  +Q TDIE DA+R+FQGT+ENLSDDPAL+IYTSGT
Sbjct: 181 SVAAKTAAKFSLIPPVPHIYSETNSRDQSTDIEMDAERVFQGTVENLSDDPALVIYTSGT 240

Query: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNLHVH 300
           TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQ LHCLPLH                 HVH
Sbjct: 241 TGKPKGVVHTHKSITSQVQTLSKAWEYTSADQLLHCLPLH-----------------HVH 300

Query: 301 GLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYARLL 360
           GLFNG LAPIYAGSTVEFMPKFSVSGIWKRWRESYPSN SK+DDAIT+FTGVPTMYARLL
Sbjct: 301 GLFNGFLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNGSKNDDAITIFTGVPTMYARLL 360

Query: 361 QGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFVMAI 420
           QGYEAM+P+LQV SASAA+QLRLMMCGSSALP P+M+QW+ VTGH+LLERYGMTEFVMAI
Sbjct: 361 QGYEAMEPDLQVDSASAARQLRLMMCGSSALPTPIMKQWKAVTGHQLLERYGMTEFVMAI 420

Query: 421 SNPLNGERKVGTVGKPFPGIEAKIVADDESSN----EGELCIRSPSMFKEYWKLPEVTKE 480
           SNPLNGERK+GTVGKP PG+EAKIV+DD S+N    EGELCIRSPSMFKEYWKLP+VTKE
Sbjct: 421 SNPLNGERKIGTVGKPLPGVEAKIVSDDGSNNDTNLEGELCIRSPSMFKEYWKLPKVTKE 480

Query: 481 SFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCV 540
           SF+DGGFFKTGDAAR DE+GYY ILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCV
Sbjct: 481 SFIDGGFFKTGDAARLDEDGYYTILGRMNADIMKVGGYKLSALEIESVILQHPSVIECCV 540

Query: 541 LGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKLLLWE 600
           LGLPDKDYGERVCAIIVL PN K TPPD SKP ++L +LRTWA+DKLAPYK+PT LLL +
Sbjct: 541 LGLPDKDYGERVCAIIVLHPNSKATPPDQSKPNMSLDDLRTWAKDKLAPYKIPTVLLLKD 593

Query: 601 SLPRNAMGKV 607
           SLPRNAMGKV
Sbjct: 601 SLPRNAMGKV 593

BLAST of MS009497 vs. ExPASy TrEMBL
Match: A0A0A0L7U7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G099620 PE=3 SV=1)

HSP 1 Score: 973.0 bits (2514), Expect = 5.7e-280
Identity = 498/614 (81.11%), Postives = 536/614 (87.30%), Query Frame = 0

Query: 1   MNSSTFKSPTFFFIRICQPPFAPSRLITTRSLSVCYNLGRSIRKLRFPRSRLYSIISSHT 60
           MN STF +PT  F RI       SRLI  RS S C+ +G+SI K  F R RL+S++SS+T
Sbjct: 1   MNYSTFNNPTSLFTRISHLSLPSSRLIAIRSSSACFCIGKSIPKFCFSRFRLFSVLSSYT 60

Query: 61  FMEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKTEHGHLGGAK 120
           FMEVVKS AKHG+T  E+VAIRANQKSHSY QLISSALKIS LLTSSD++T HGHLGGAK
Sbjct: 61  FMEVVKSAAKHGATCRENVAIRANQKSHSYIQLISSALKISDLLTSSDLRTGHGHLGGAK 120

Query: 121 VGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDRGEFMQ 180
           +GIVAKPSAEFLAGVLGTW SGGVAVPLALSYPEAELLHVLNDSEVSMILSTED  EFMQ
Sbjct: 121 IGIVAKPSAEFLAGVLGTWFSGGVAVPLALSYPEAELLHVLNDSEVSMILSTEDHVEFMQ 180

Query: 181 SVAAKTAAKFSLIPPVPHISSERNPHEQPTDIE---ADADRIFQGTIENLSDDPALIIYT 240
           SVAAKT A  SLIPPVPHI SERN H+Q T+IE   ADADR+FQGT+ENLSDDPALIIYT
Sbjct: 181 SVAAKTVATVSLIPPVPHIYSERNSHDQSTNIEVDAADADRVFQGTVENLSDDPALIIYT 240

Query: 241 SGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVSPYFNL 300
           SGTTGKPKGVVHTHKSITSQVQ LS AWEYTSADQFLHCLPLH                 
Sbjct: 241 SGTTGKPKGVVHTHKSITSQVQMLSNAWEYTSADQFLHCLPLH----------------- 300

Query: 301 HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGVPTMYA 360
           HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPS SSKSDDAITVFTGVPTMYA
Sbjct: 301 HVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSKSSKSDDAITVFTGVPTMYA 360

Query: 361 RLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYGMTEFV 420
           RLLQGYEAMD ELQ+ASASAA+QLRLMMCGSSALPPP+M+QW++VTGH LLERYGMTEFV
Sbjct: 361 RLLQGYEAMDSELQLASASAARQLRLMMCGSSALPPPIMKQWKEVTGHELLERYGMTEFV 420

Query: 421 MAISNPLNGERKVGTVGKPFPGIEAKIVADDESSN----EGELCIRSPSMFKEYWKLPEV 480
           MAISNPLNG+RK+GTVGKPFPG+EAKIVADD S+N    EGELCIRSPSMFKEYWKLPEV
Sbjct: 421 MAISNPLNGQRKIGTVGKPFPGVEAKIVADDGSTNDSSLEGELCIRSPSMFKEYWKLPEV 480

Query: 481 TKESFLDGGFFKTGDAARRDEN-GYYIILGRMNADIMKVGGYKLSALEIESVILQHPSVI 540
           TKESF+DGGFFKTGDAAR DE+ GYYIILGR++ADIMKVGGYKLSALEIESVILQHPSVI
Sbjct: 481 TKESFIDGGFFKTGDAARLDEDGGYYIILGRLSADIMKVGGYKLSALEIESVILQHPSVI 540

Query: 541 ECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTKL 600
           ECCVLGLPDKDYGERVCAI+VL+ N K T PD S P+++L ELRTWA+DKLAPYKLPT L
Sbjct: 541 ECCVLGLPDKDYGERVCAIVVLQANMKTTIPDGSNPSMSLDELRTWAKDKLAPYKLPTML 597

Query: 601 LLWESLPRNAMGKV 607
           LL +SLPRNAMGKV
Sbjct: 601 LLKDSLPRNAMGKV 597

BLAST of MS009497 vs. TAIR 10
Match: AT3G16170.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 712.2 bits (1837), Expect = 3.5e-205
Identity = 362/555 (65.23%), Postives = 424/555 (76.40%), Query Frame = 0

Query: 62  MEVVKSTAKHGSTFSESVAIRANQKSHSYGQLISSALKISALLTSSDIKT---------E 121
           MEV K+     S   + +AI+A+ KS+SYGQL SSAL+IS L    D             
Sbjct: 1   MEVFKAAFSEASNSCDRIAIKADGKSYSYGQLTSSALRISKLFLKDDTTNGGQETKKYEG 60

Query: 122 HGHLGGAKVGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSEVSMILST 181
            G L GA++GIVAKPSAEF+AGVLGTW SGGVAVPLALSYPEAELLHV+NDS++S++LST
Sbjct: 61  FGSLKGARIGIVAKPSAEFVAGVLGTWFSGGVAVPLALSYPEAELLHVMNDSDISLLLST 120

Query: 182 EDRGEFMQSVAAKTAAKFSLIPPVPHISSERNPHEQPTDIEADADRIFQGTIENLSDDPA 241
           ED  E M+++AAK+ A+F LIPPV + +SE     Q  D   +A+  F        DDPA
Sbjct: 121 EDHSETMKTIAAKSGARFHLIPPVVNSTSETVACNQFQDDSFEAEGKF-------LDDPA 180

Query: 242 LIIYTSGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFSTFVAVS 301
           LI+YTSGTTGKPKGVVHTH SI SQV+ L++AWEYTSAD FLHCLPLH            
Sbjct: 181 LIVYTSGTTGKPKGVVHTHNSINSQVRMLTEAWEYTSADHFLHCLPLH------------ 240

Query: 302 PYFNLHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAITVFTGV 361
                HVHGLFN L AP+YA S VEF+PKFSVSGIW+RWRESYP N  K++D+ITVFTGV
Sbjct: 241 -----HVHGLFNALFAPLYARSLVEFLPKFSVSGIWRRWRESYPVNDEKTNDSITVFTGV 300

Query: 362 PTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDVTGHRLLERYG 421
           PTMY RL+QGYEAMD E+Q +SA AA++LRLMM GSSALP PVM QWE +TGHRLLERYG
Sbjct: 301 PTMYTRLIQGYEAMDKEMQDSSAFAARKLRLMMSGSSALPRPVMHQWESITGHRLLERYG 360

Query: 422 MTEFVMAISNPLNGERKVGTVGKPFPGIEAKIVADDESSN-EGELCIRSPSMFKEYWKLP 481
           MTEFVMA+SNPL G R  GTVGKP PG+EAKI  D+  +N  GE+C++SPS+FKEYW LP
Sbjct: 361 MTEFVMAMSNPLRGARNAGTVGKPLPGVEAKIKEDENDANGVGEICVKSPSLFKEYWNLP 420

Query: 482 EVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSALEIESVILQHPSV 541
           EVTKESF + G+FKTGDA R DE+GYY+ILGR +ADIMKVGGYKLSALEIES +L+HP+V
Sbjct: 421 EVTKESFTEDGYFKTGDAGRVDEDGYYVILGRNSADIMKVGGYKLSALEIESTLLEHPTV 480

Query: 542 IECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWARDKLAPYKLPTK 601
            ECCVLGL D DYGE V AII+ E   K+   D+SKP ITL EL  WA+DKLAPYKLPT+
Sbjct: 481 AECCVLGLTDNDYGEAVTAIIIAESAAKKRREDESKPVITLEELCGWAKDKLAPYKLPTR 531

Query: 602 LLLWESLPRNAMGKV 607
           LL+WESLPRNAMGKV
Sbjct: 541 LLIWESLPRNAMGKV 531

BLAST of MS009497 vs. TAIR 10
Match: AT3G48990.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 200.3 bits (508), Expect = 4.5e-51
Identity = 150/514 (29.18%), Postives = 253/514 (49.22%), Query Frame = 0

Query: 106 SSDIKTEHGHLGGAKVGIVAKPSAEFLAGVLGTWLSGGVAVPLALSYPEAELLHVLNDSE 165
           +S + ++ G   G  V +    + EF+   L    +   A PL  +Y   E    L+DS+
Sbjct: 44  ASRLVSDAGIKPGDVVALTFPNTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSD 103

Query: 166 VSMILSTEDRGEFMQSVAAKTAAKFSLIPPVPHISSERNPHEQPTDIE-ADADRIFQGTI 225
             ++L++++     Q  A+K          + H+++          +  AD+D +     
Sbjct: 104 SKLLLTSKEGNAPAQEAASKL--------KISHVTATLLDAGSDLVLSVADSDSVVDSAT 163

Query: 226 E--NLSDDPALIIYTSGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHI 285
           E  N  DD AL ++TSGTT +PKGV  T  ++ S V+ +   ++ T +D  +  LPL   
Sbjct: 164 ELVNHPDDGALFLHTSGTTSRPKGVPLTQLNLASSVKNIKAVYKLTESDSTVIVLPL--- 223

Query: 286 LEFSTFVAVSPYFNLHVHGLFNGLLAPIYAGS--TVEFMPKFSVSGIWKRWRESYPSNSS 345
                          HVHGL  GLL+ + AG+  T+    +FS +  W   ++       
Sbjct: 224 --------------FHVHGLLAGLLSSLGAGAAVTLPAAGRFSATTFWPDMKKY------ 283

Query: 346 KSDDAITVFTGVPTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWE 405
                 T +T VPT++  +L  + A  PE +        +LR +   S++L P ++ + E
Sbjct: 284 ----NATWYTAVPTIHQIILDRH-ASHPETEY------PKLRFIRSCSASLAPVILSRLE 343

Query: 406 DVTGHRLLERYGMTEFV-MAISNPL--NGERKVGTVGKPFPGIEAKIVAD----DESSNE 465
           +  G  +LE Y MTE   +  SNPL   G  K G+VGKP  G E  I+ +     E +N+
Sbjct: 344 EAFGAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKPV-GQEMAILNEKGEIQEPNNK 403

Query: 466 GELCIRSPSMFKEYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGG 525
           GE+CIR P++ K Y   PE  K  F + G+F TGD    D +GY  ++GR+  +++  GG
Sbjct: 404 GEVCIRGPNVTKGYKNNPEANKAGF-EFGWFHTGDIGYFDTDGYLHLVGRIK-ELINRGG 463

Query: 526 YKLSALEIESVILQHPSVIECCVLGLPDKDYGERV-CAIIVLEPNFKRTPPDDSKPAITL 585
            K+S +E+++V+L HP V +    G+PD+ YGE + CA+I  E              +T 
Sbjct: 464 EKISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGT-----------TVTE 501

Query: 586 LELRTWARDKLAPYKLPTKLLLWESLPRNAMGKV 607
            +++ + +  LA +K+P ++ + ++LP+ A GK+
Sbjct: 524 EDIKAFCKKNLAAFKVPKRVFITDNLPKTASGKI 501

BLAST of MS009497 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 142.5 bits (358), Expect = 1.1e-33
Identity = 108/394 (27.41%), Postives = 194/394 (49.24%), Query Frame = 0

Query: 229 DDPALIIYTSGTTGKPKGVVHTHKSITSQVQTL----SKAWEYT-SADQFLHCLPLHHIL 288
           DD A I+Y+SGTTG  KGV+ TH+++ + ++      +  +EY  S++ +L  LPL HI 
Sbjct: 204 DDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLCHIY 263

Query: 289 EFSTFVAVSPYFNLHVHGLFNGLLAPIYAGSTVEFMPKFSVS---GIWKRWRESYPSNSS 348
             S FV               GLL+    GST+  M +F  S    + +R++        
Sbjct: 264 GLSLFVM--------------GLLS---LGSTIVVMKRFDASDVVNVIERFK-------- 323

Query: 349 KSDDAITVFTGVPTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWE 408
                IT F  VP M   L +  + +  E+        K L+ +  G++ L    ++ + 
Sbjct: 324 -----ITHFPVVPPMLMALTKKAKGVCGEV-------FKSLKQVSSGAAPLSRKFIEDFL 383

Query: 409 DVTGH-RLLERYGMTEFVMAISNPLNGER--KVGTVGKPFPGIEAKIVADDESS-----N 468
               H  L++ YGMTE     +   N E+  +  +VG   P ++AK+V     S     N
Sbjct: 384 QTLPHVDLIQGYGMTESTAVGTRGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGN 443

Query: 469 EGELCIRSPSMFKEYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVG 528
            GEL I+ P + K Y   P+ T+ S ++  + +TGD A  DE+GY  I+ R+  +I+K  
Sbjct: 444 RGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRTGDIAYFDEDGYLFIVDRIK-EIIKYK 503

Query: 529 GYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITL 588
           G++++  ++E+V++ HP +I+  V   P+++ GE   A +V             +  ++ 
Sbjct: 504 GFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVAFVVRR----------QETTLSE 549

Query: 589 LELRTWARDKLAPYKLPTKLLLWESLPRNAMGKV 607
            ++ ++   ++APY+   K+++  S+P++  GK+
Sbjct: 564 EDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKI 549

BLAST of MS009497 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 142.1 bits (357), Expect = 1.4e-33
Identity = 102/387 (26.36%), Postives = 185/387 (47.80%), Query Frame = 0

Query: 229 DDPALIIYTSGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQ-FLHCLPLHHILEFST 288
           DD A ++Y+SGTTG  KGV+ +H+++ + VQT+   +     +Q F+  +P+ HI   + 
Sbjct: 189 DDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHIYGLAA 248

Query: 289 FVAVSPYFNLHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAIT 348
           F                GLLA    GST+  + KF          E +   S+      T
Sbjct: 249 FA--------------TGLLA---YGSTIIVLSKF----------EMHEMMSAIGKYQAT 308

Query: 349 VFTGVPTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQW-EDVTGHR 408
               VP +   ++ G + +  +  ++S      +  ++CG + L   V + + E     +
Sbjct: 309 SLPLVPPILVAMVNGADQIKAKYDLSS------MHTVLCGGAPLSKEVTEGFAEKYPTVK 368

Query: 409 LLERYGMTEF--VMAISNPLNGERKVGTVGKPFPGIEAKIVADDES-----SNEGELCIR 468
           +L+ YG+TE   + A ++ +   R+ GT GK    +E +IV             GEL ++
Sbjct: 369 ILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLK 428

Query: 469 SPSMFKEYWKLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMNADIMKVGGYKLSAL 528
            PS+ K Y+   E T  +    G+ +TGD    DE+G+  ++ R+  +++K  GY+++  
Sbjct: 429 GPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLK-ELIKYKGYQVAPA 488

Query: 529 EIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDDSKPAITLLELRTWA 588
           E+E+++L HP + +  V+  PDK+ G+   A +V +          S    T++E   + 
Sbjct: 489 ELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTG-------SSLSEKTIME---FV 531

Query: 589 RDKLAPYKLPTKLLLWESLPRNAMGKV 607
             ++APYK   K+    S+P+N  GK+
Sbjct: 549 AKQVAPYKRIRKVAFVSSIPKNPSGKI 531

BLAST of MS009497 vs. TAIR 10
Match: AT1G30520.1 (acyl-activating enzyme 14 )

HSP 1 Score: 133.3 bits (334), Expect = 6.7e-31
Identity = 110/405 (27.16%), Postives = 178/405 (43.95%), Query Frame = 0

Query: 228 SDDPALIIYTSGTTGKPKGVVHTHKSITSQVQTLSKAWEYTSADQFLHCLPLHHILEFST 287
           SDD  +I +TSGTTG+PKGV  +H +  +Q         Y   D +LH  PL HI     
Sbjct: 171 SDDAVVICFTSGTTGRPKGVTISHLAFITQSLAKIAIAGYGEDDVYLHTSPLVHI----- 230

Query: 288 FVAVSPYFNLHVHGLFNGLLAPIYAGSTVEFMPKFSVSGIWKRWRESYPSNSSKSDDAIT 347
                        G  +  +A +  G+    +PKF      +   +++          IT
Sbjct: 231 -------------GGLSSAMAMLMVGACHVLLPKFDAKTALQVMEQNH----------IT 290

Query: 348 VFTGVPTMYARLLQGYEAMDPELQVASASAAKQLRLMMCGSSALPPPVMQQWEDV-TGHR 407
            F  VP M A L++            + +  + +R ++ G  +L   ++++  ++    R
Sbjct: 291 CFITVPAMMADLIR------VNRTTKNGAENRGVRKILNGGGSLSSELLKEAVNIFPCAR 350

Query: 408 LLERYGMTEFVMA---------------ISNPLNGERKVGT-VGKPFPGIEAKIVADDES 467
           +L  YGMTE   +               ++ PL  + K GT VGKP P IE  +  D++S
Sbjct: 351 ILSAYGMTEACSSLTFMTLHDPTQESFKVTYPLLNQPKQGTCVGKPAPHIELMVKLDEDS 410

Query: 468 SNEGELCIRSPSMFKEYW-----KLPEVTKESFLDGGFFKTGDAARRDENGYYIILGRMN 527
           S  G++  R P     YW     +    T ES  +  +  TGD    DE G   ++GR N
Sbjct: 411 SRVGKILTRGPHTMLRYWGHQVAQENVETSESRSNEAWLDTGDIGAFDEFGNLWLIGRSN 470

Query: 528 ADIMKVGGYKLSALEIESVILQHPSVIECCVLGLPDKDYGERVCAIIVLEPNFKRTPPDD 587
             I K GG  +   E+E+V+++HP ++   V+G+ D   GE V A + L+  +  +  ++
Sbjct: 471 GRI-KTGGENVYPEEVEAVLVEHPGIVSAVVIGVIDTRLGEMVVACVRLQEKWIWSDVEN 530

Query: 588 SKPAITLLE--LRTWAR-DKLAPYKLPTKLLLWE-SLPRNAMGKV 607
            K +  L    L+   R   L  +K+P + + WE   P    GKV
Sbjct: 531 RKGSFQLSSETLKHHCRTQNLTGFKIPKRFVRWEKQFPLTTTGKV 540