MS006871.1 (mRNA) Bitter gourd (TR) v1

Overview
NameMS006871.1
TypemRNA
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMajor facilitator superfamily protein
Locationscaffold871: 300563 .. 302218 (+)
Sequence length1656
RNA-Seq ExpressionMS006871.1
SyntenyMS006871.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTGCAGCAGAAGGTTGGCGATTCGCGAAACAAGTTGCCGATGGGCGATGGTTTTCGGTGTTCGCTTCGTTCGTGATCATGATCGGCGCCGGCTCACCCTACCTATTCGGAACATACTCCAAAGTCATAAAGACCCAATTCGATTACAGCCAAACCCAAGTTAACACCTTGGGATTCTCCAAAGATCTCGGCTCCAACCTCGGAGTCTTCGCCGGACTTCTCGCCGAGGTGGCTCCGCCGTGGGTTCTGTTCGTCGTCGGCTCCTCCCTCAACTTCTTCAGCTTCATCATGATTTGGCTCTCCGTCACTCACCGGATCGCCAAGCCCGAGTTCTGGCAGATGTTCCTCTACGTCGGCTTGGCTGCCAACGCACAAAACTTCGCCAACACTGCGGTTATGGTCATCAGCGTTAAAAACTTCCCGGACCGACGAGGGATTATTATTGGCCTTCTCAAGGGCTTCGTCGGCCTCGGAGGGGCCGTCTTGACTCAGATCTATCTAGCCATTTATGGCCACGAAGATCCATCTGATGTGCTTCTTCTGCTCTCATGGTTTCCGTCCGTTATATCCATTGTTCTTTTGTTTTCAATTAGAACAATCAGAGCTCGCAAACATCCGGAGGAGCTTAAAGTGTTTTACCACCTGCTGTACGCATCCATAATTCTAGCCATCTCTATCCTTATTATTACCTTGACCCAAAAACACATTATTTTCTCCCATGCTGCCTATTTGGGAAGCGTCGCCGTGGTTGTGGCCTCACTGTGTCTGCCCCTTCTGATTGCCATTAAAGAAGAATTCTTGCTTTTCAAGCTCAACAACCAAGCCGTGGATCCATCTGTCACCCTTTCCATTCCAGAGGAGAAGCAATCTCTGCCGTACTCCAATATCTTACTATCCACAGTACTTGAAAAAGATTCCCCTTCTAAGCCAGAAAGAGGGGAAGATTTCACCATCTTGCAAGCTCTCCTCAGCAAAGACATGGCTCTTGTTTTCTTGGCAACTGTTTCTGCATGTGGGTCCTCCATTGTCGCCATCGACAATCTTGGACAAATCGCAGAATCACTTGCCTATCCTGCTCAATCCATAAGCGTCTTCGTTTCTTGGATTTCCATATTCAATTTCTTCGGTCGGGTCTTCTCTGGCTTCATCTCCGAAACGCTAATGACCAAATACAAATTACCTCGTCCTCTCATGTTCGCCCTTGCCCATCTCCTCACCTGCATTGGCATACTCTTCATCGCCTTTCCATATCATGGTTCGGTCTACGTGGCTTCATTGATCATTGGGTCTGGATTTGGAGCCCAAGTTCCTTTGTTATTCGCTATTATCTCTGATCTATTTGGCCTCAAGCACTACTCAACGCTGCTCAATTGTGGGCAACTGGCGGTGCCGCTTGGATCTTATATACTAAATGTACTTCTAGTAGGTAGGATGTATGATATGGAAGCCACTCGAGCTGGTAATACAAAGCACGGCAAAGGCCTAACTTGCAATGGACCTCATTGCTTTGGCGGCTCGTTCACAATTTTGGCAGCAATAACATTGTTTGGAGCAATGAGTTCACTTGCACTGGCTTGTAGGACTCGAAACTTTTACAAAGGCGACATATATAAGAGATATAGAGATGACATGTGGATTACCCAATCCGACATG

mRNA sequence

ATGTGTGCAGCAGAAGGTTGGCGATTCGCGAAACAAGTTGCCGATGGGCGATGGTTTTCGGTGTTCGCTTCGTTCGTGATCATGATCGGCGCCGGCTCACCCTACCTATTCGGAACATACTCCAAAGTCATAAAGACCCAATTCGATTACAGCCAAACCCAAGTTAACACCTTGGGATTCTCCAAAGATCTCGGCTCCAACCTCGGAGTCTTCGCCGGACTTCTCGCCGAGGTGGCTCCGCCGTGGGTTCTGTTCGTCGTCGGCTCCTCCCTCAACTTCTTCAGCTTCATCATGATTTGGCTCTCCGTCACTCACCGGATCGCCAAGCCCGAGTTCTGGCAGATGTTCCTCTACGTCGGCTTGGCTGCCAACGCACAAAACTTCGCCAACACTGCGGTTATGGTCATCAGCGTTAAAAACTTCCCGGACCGACGAGGGATTATTATTGGCCTTCTCAAGGGCTTCGTCGGCCTCGGAGGGGCCGTCTTGACTCAGATCTATCTAGCCATTTATGGCCACGAAGATCCATCTGATGTGCTTCTTCTGCTCTCATGGTTTCCGTCCGTTATATCCATTGTTCTTTTGTTTTCAATTAGAACAATCAGAGCTCGCAAACATCCGGAGGAGCTTAAAGTGTTTTACCACCTGCTGTACGCATCCATAATTCTAGCCATCTCTATCCTTATTATTACCTTGACCCAAAAACACATTATTTTCTCCCATGCTGCCTATTTGGGAAGCGTCGCCGTGGTTGTGGCCTCACTGTGTCTGCCCCTTCTGATTGCCATTAAAGAAGAATTCTTGCTTTTCAAGCTCAACAACCAAGCCGTGGATCCATCTGTCACCCTTTCCATTCCAGAGGAGAAGCAATCTCTGCCGTACTCCAATATCTTACTATCCACAGTACTTGAAAAAGATTCCCCTTCTAAGCCAGAAAGAGGGGAAGATTTCACCATCTTGCAAGCTCTCCTCAGCAAAGACATGGCTCTTGTTTTCTTGGCAACTGTTTCTGCATGTGGGTCCTCCATTGTCGCCATCGACAATCTTGGACAAATCGCAGAATCACTTGCCTATCCTGCTCAATCCATAAGCGTCTTCGTTTCTTGGATTTCCATATTCAATTTCTTCGGTCGGGTCTTCTCTGGCTTCATCTCCGAAACGCTAATGACCAAATACAAATTACCTCGTCCTCTCATGTTCGCCCTTGCCCATCTCCTCACCTGCATTGGCATACTCTTCATCGCCTTTCCATATCATGGTTCGGTCTACGTGGCTTCATTGATCATTGGGTCTGGATTTGGAGCCCAAGTTCCTTTGTTATTCGCTATTATCTCTGATCTATTTGGCCTCAAGCACTACTCAACGCTGCTCAATTGTGGGCAACTGGCGGTGCCGCTTGGATCTTATATACTAAATGTACTTCTAGTAGGTAGGATGTATGATATGGAAGCCACTCGAGCTGGTAATACAAAGCACGGCAAAGGCCTAACTTGCAATGGACCTCATTGCTTTGGCGGCTCGTTCACAATTTTGGCAGCAATAACATTGTTTGGAGCAATGAGTTCACTTGCACTGGCTTGTAGGACTCGAAACTTTTACAAAGGCGACATATATAAGAGATATAGAGATGACATGTGGATTACCCAATCCGACATG

Coding sequence (CDS)

ATGTGTGCAGCAGAAGGTTGGCGATTCGCGAAACAAGTTGCCGATGGGCGATGGTTTTCGGTGTTCGCTTCGTTCGTGATCATGATCGGCGCCGGCTCACCCTACCTATTCGGAACATACTCCAAAGTCATAAAGACCCAATTCGATTACAGCCAAACCCAAGTTAACACCTTGGGATTCTCCAAAGATCTCGGCTCCAACCTCGGAGTCTTCGCCGGACTTCTCGCCGAGGTGGCTCCGCCGTGGGTTCTGTTCGTCGTCGGCTCCTCCCTCAACTTCTTCAGCTTCATCATGATTTGGCTCTCCGTCACTCACCGGATCGCCAAGCCCGAGTTCTGGCAGATGTTCCTCTACGTCGGCTTGGCTGCCAACGCACAAAACTTCGCCAACACTGCGGTTATGGTCATCAGCGTTAAAAACTTCCCGGACCGACGAGGGATTATTATTGGCCTTCTCAAGGGCTTCGTCGGCCTCGGAGGGGCCGTCTTGACTCAGATCTATCTAGCCATTTATGGCCACGAAGATCCATCTGATGTGCTTCTTCTGCTCTCATGGTTTCCGTCCGTTATATCCATTGTTCTTTTGTTTTCAATTAGAACAATCAGAGCTCGCAAACATCCGGAGGAGCTTAAAGTGTTTTACCACCTGCTGTACGCATCCATAATTCTAGCCATCTCTATCCTTATTATTACCTTGACCCAAAAACACATTATTTTCTCCCATGCTGCCTATTTGGGAAGCGTCGCCGTGGTTGTGGCCTCACTGTGTCTGCCCCTTCTGATTGCCATTAAAGAAGAATTCTTGCTTTTCAAGCTCAACAACCAAGCCGTGGATCCATCTGTCACCCTTTCCATTCCAGAGGAGAAGCAATCTCTGCCGTACTCCAATATCTTACTATCCACAGTACTTGAAAAAGATTCCCCTTCTAAGCCAGAAAGAGGGGAAGATTTCACCATCTTGCAAGCTCTCCTCAGCAAAGACATGGCTCTTGTTTTCTTGGCAACTGTTTCTGCATGTGGGTCCTCCATTGTCGCCATCGACAATCTTGGACAAATCGCAGAATCACTTGCCTATCCTGCTCAATCCATAAGCGTCTTCGTTTCTTGGATTTCCATATTCAATTTCTTCGGTCGGGTCTTCTCTGGCTTCATCTCCGAAACGCTAATGACCAAATACAAATTACCTCGTCCTCTCATGTTCGCCCTTGCCCATCTCCTCACCTGCATTGGCATACTCTTCATCGCCTTTCCATATCATGGTTCGGTCTACGTGGCTTCATTGATCATTGGGTCTGGATTTGGAGCCCAAGTTCCTTTGTTATTCGCTATTATCTCTGATCTATTTGGCCTCAAGCACTACTCAACGCTGCTCAATTGTGGGCAACTGGCGGTGCCGCTTGGATCTTATATACTAAATGTACTTCTAGTAGGTAGGATGTATGATATGGAAGCCACTCGAGCTGGTAATACAAAGCACGGCAAAGGCCTAACTTGCAATGGACCTCATTGCTTTGGCGGCTCGTTCACAATTTTGGCAGCAATAACATTGTTTGGAGCAATGAGTTCACTTGCACTGGCTTGTAGGACTCGAAACTTTTACAAAGGCGACATATATAAGAGATATAGAGATGACATGTGGATTACCCAATCCGACATG

Protein sequence

MCAAEGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHAAYLGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLSTVLEKDSPSKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAYPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVGRMYDMEATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGDIYKRYRDDMWITQSDM
Homology
BLAST of MS006871.1 vs. NCBI nr
Match: XP_022152848.1 (uncharacterized membrane protein YMR155W-like [Momordica charantia])

HSP 1 Score: 1061.2 bits (2743), Expect = 3.0e-306
Identity = 547/552 (99.09%), Postives = 549/552 (99.46%), Query Frame = 0

Query: 1   MCAAEGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGF 60
           MCAAEGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGF
Sbjct: 4   MCAAEGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGF 63

Query: 61  SKDLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVG 120
           SKDLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVG
Sbjct: 64  SKDLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVG 123

Query: 121 LAANAQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVL 180
           LAANAQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVL
Sbjct: 124 LAANAQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVL 183

Query: 181 LLLSWFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFS 240
           LLLSWFPSVISIVLLFSIRTIRAR HPEELKVFYHLLYASIILAISILIITLTQKHI+FS
Sbjct: 184 LLLSWFPSVISIVLLFSIRTIRARXHPEELKVFYHLLYASIILAISILIITLTQKHIVFS 243

Query: 241 HAAYLGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLS 300
           HAAYLGSVAVVVA LCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLS
Sbjct: 244 HAAYLGSVAVVVALLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLS 303

Query: 301 TVLEKDSPSKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAYPA 360
           TVLEKDSPSKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAYPA
Sbjct: 304 TVLEKDSPSKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAYPA 363

Query: 361 QSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHG 420
           QSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHG
Sbjct: 364 QSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHG 423

Query: 421 SVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVGRMY 480
           SVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVGRMY
Sbjct: 424 SVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVGRMY 483

Query: 481 DMEATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGDIYKR 540
           DMEA RAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGDIYKR
Sbjct: 484 DMEANRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGDIYKR 543

Query: 541 YRDDMWITQSDM 553
           YRDDMWIT+SDM
Sbjct: 544 YRDDMWITESDM 555

BLAST of MS006871.1 vs. NCBI nr
Match: XP_022152849.1 (uncharacterized protein LOC111020473 [Momordica charantia])

HSP 1 Score: 800.4 bits (2066), Expect = 9.5e-228
Identity = 406/550 (73.82%), Postives = 464/550 (84.36%), Query Frame = 0

Query: 3   AAEGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSK 62
           AA  W F KQVA+GRWFSVFASF+IMIGAGS YLFGTYSK++KTQFDY QTQVNTLGF+K
Sbjct: 7   AAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGFAK 66

Query: 63  DLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLA 122
           DLGSN GVFAGLLAEVAP WVLF+VGSSLNFFS+ MIWLSVT RIA P+FW M LY+ LA
Sbjct: 67  DLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYICLA 126

Query: 123 ANAQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLL 182
           AN+QNFANTAV+V SVKNFP+RRGII+GLLKGFVG+GGA+LTQ+YLAIYGHE+ + V+LL
Sbjct: 127 ANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENQASVILL 186

Query: 183 LSWFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHA 242
           L+WFPSVIS V + SIRTI+ RKHPEELKVFYHLLY SIILA+ IL +TLTQK  +FSHA
Sbjct: 187 LAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVFSHA 246

Query: 243 AYLGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLSTV 302
            Y+G  +V+V  LCLPLLIAI+EEF+LF LN Q  D  V + I ++K   P    L    
Sbjct: 247 GYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKP--PPKRALEEDA 306

Query: 303 LEKDSPSKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAYPAQS 362
           ++  S  KPERG+DFTI QALLSKDMAL+F+AT+S CGSSI AIDN+GQIAESL YP  S
Sbjct: 307 IDSVS-IKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQYPPHS 366

Query: 363 ISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSV 422
           I++FVSW+SIFNFFGRVFSGFISETLM KYKLPRPLMFA + LLTCIG L IAFPY GSV
Sbjct: 367 IAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPYRGSV 426

Query: 423 YVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVGRMYDM 482
           Y ASLIIG GFGAQVPLLFAIIS+LFGLKHYSTL NCGQLAVP GSYILNV ++G++YDM
Sbjct: 427 YAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGKLYDM 486

Query: 483 EATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGDIYKRYR 542
           EA R G  K GKGLTC G +CF  SFTILAA+T FGA+SSL LA RTR+FYKG+ YK+YR
Sbjct: 487 EAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFFGALSSLVLAYRTRDFYKGETYKKYR 546

Query: 543 DDMWITQSDM 553
           +DMWI ++DM
Sbjct: 547 EDMWIPKADM 553

BLAST of MS006871.1 vs. NCBI nr
Match: XP_038895745.1 (uncharacterized protein LOC120083909 [Benincasa hispida])

HSP 1 Score: 781.6 bits (2017), Expect = 4.6e-222
Identity = 393/556 (70.68%), Postives = 458/556 (82.37%), Query Frame = 0

Query: 4   AEGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKD 63
           AE WRF KQV +GRWFSVFA+F+IMIG GS YLFGTYSKV+KT+FDY+QTQ++ LGF+KD
Sbjct: 7   AENWRFVKQVVEGRWFSVFAAFLIMIGCGSTYLFGTYSKVLKTKFDYNQTQLSHLGFAKD 66

Query: 64  LGSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAA 123
           LGSNLGVFAGL AEVAPPW+LF+VG +LNFFS+ MIWLSVT  + KPE W MF+Y+ ++A
Sbjct: 67  LGSNLGVFAGLFAEVAPPWMLFLVGLTLNFFSYFMIWLSVTEYVPKPELWLMFIYIYISA 126

Query: 124 NAQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLL 183
           NAQNFANTAVMV SV+NFPD+RGII+GLLKGFVGLGGA+LTQIY AIYGH+DP +++LLL
Sbjct: 127 NAQNFANTAVMVTSVRNFPDQRGIILGLLKGFVGLGGAILTQIYFAIYGHQDPINLVLLL 186

Query: 184 SWFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHAA 243
           SW PS+I  +   S RTI+ARKHP+ELKVF+HLLY SI +A+ IL +T+TQKH  F+HA 
Sbjct: 187 SWLPSIICFLFFLSFRTIKARKHPQELKVFFHLLYVSITMAVFILFLTITQKHTPFTHAN 246

Query: 244 YLGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLSTVL 303
           Y+G  +V+V  LCLPLLIAIKEE  LFKLN Q  DPSV +SIP +K         L  + 
Sbjct: 247 YVGGTSVIVVLLCLPLLIAIKEELFLFKLNKQTKDPSVVVSIPVQK---------LEEIS 306

Query: 304 EKDSP-------SKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESL 363
           E  SP       +KP+RGEDF I+QAL SKDMAL+F+ATVSACGSS+ AIDNLGQIAESL
Sbjct: 307 ETSSPCFSNKRSNKPQRGEDFGIVQALFSKDMALIFIATVSACGSSVAAIDNLGQIAESL 366

Query: 364 AYPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAF 423
            YP+++I+VFVSWISIFNFFGRV SGF+SETLMTKYKLPRPLMF L  ++T IG++ IAF
Sbjct: 367 NYPSETINVFVSWISIFNFFGRVCSGFVSETLMTKYKLPRPLMFGLTQVITSIGLISIAF 426

Query: 424 PYHGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLV 483
           PY  SVY ASLIIG GFGAQ PLLFA+ISDLFGLKHYSTLLNCGQLAVP GSYI+NV +V
Sbjct: 427 PYKDSVYAASLIIGFGFGAQTPLLFALISDLFGLKHYSTLLNCGQLAVPFGSYIMNVHVV 486

Query: 484 GRMYDMEATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGD 543
           G+ YD EAT+ GN K+GKGLTC G HCF  SF IL  +TLFGAM+S  LA RTR FYKGD
Sbjct: 487 GKFYDREATKIGNVKNGKGLTCTGTHCFSESFQILVLVTLFGAMTSFVLAYRTREFYKGD 546

Query: 544 IYKRYRDD-MWITQSD 552
           IYKRYRDD MW TQSD
Sbjct: 547 IYKRYRDDQMWSTQSD 553

BLAST of MS006871.1 vs. NCBI nr
Match: XP_022970242.1 (uncharacterized protein LOC111469251 [Cucurbita maxima])

HSP 1 Score: 777.3 bits (2006), Expect = 8.7e-221
Identity = 378/556 (67.99%), Postives = 468/556 (84.17%), Query Frame = 0

Query: 5   EGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDL 64
           E WRF KQV +GRWF+VFASF+IMIGAGS Y+FGTYSK IK+QFDY+QTQ+NTLGF+KDL
Sbjct: 8   ESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDL 67

Query: 65  GSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAAN 124
           GSN GVFAGLL EVAPPWVLF+VGS+LNF+S+ MIWLS+T RI KP FW+MF+++ +AAN
Sbjct: 68  GSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRIVKPPFWEMFVFICVAAN 127

Query: 125 AQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLS 184
           +QNFANTA++V SV+NFPDRRGII+GLLKGFVG GGA+LTQ YLA+YGH++P++++LLLS
Sbjct: 128 SQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLS 187

Query: 185 WFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHAAY 244
           W P+V+S++   SIR I+ RKHP+EL+V YHLLY SIILA+ +L +T+TQK  +F+  AY
Sbjct: 188 WLPTVVSMLFFLSIRPIKVRKHPDELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAY 247

Query: 245 LGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAV--DPSVTLSIPEEKQSLPYSNILLSTV 304
           +    V++  L +PLLIAI+EEFLLFKLN Q +  +P+V + +PE + S P +   +  +
Sbjct: 248 MSGATVIIGLLLIPLLIAIREEFLLFKLNKQTLNNNPTVPILMPEHEPS-PKAPPTIEQI 307

Query: 305 LEKDSP------SKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESL 364
            E  +        +P+RGEDFTILQAL SKDMALVF+AT+SACG+SI AIDNLGQ+ ESL
Sbjct: 308 PEASTSCFSMAFKRPQRGEDFTILQALFSKDMALVFIATLSACGASIAAIDNLGQVGESL 367

Query: 365 AYPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAF 424
            YP+Q+IS+FVSW+SIFNFFGRVFSGFIS+ LMTKYKLPRPLMFA +H+LTC+GILFIAF
Sbjct: 368 GYPSQTISIFVSWVSIFNFFGRVFSGFISDALMTKYKLPRPLMFAFSHILTCVGILFIAF 427

Query: 425 PYHGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLV 484
           PY GSVY ASLIIG GFGAQVP+LFAIIS+LFGLK+YST+ NCGQLAVP+GSYILNV ++
Sbjct: 428 PYKGSVYAASLIIGFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVI 487

Query: 485 GRMYDMEATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGD 544
           GR+YD+EA + G  K+GKGLTC G  CF GSF ILA + LFGA++SL LA RTR+FYKGD
Sbjct: 488 GRLYDIEAAKLGGVKNGKGLTCTGTPCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGD 547

Query: 545 IYKRYRDDMWITQSDM 553
           +Y RYR+DMWI QSDM
Sbjct: 548 VYNRYREDMWIPQSDM 562

BLAST of MS006871.1 vs. NCBI nr
Match: KAG6583549.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 777.3 bits (2006), Expect = 8.7e-221
Identity = 380/556 (68.35%), Postives = 468/556 (84.17%), Query Frame = 0

Query: 5   EGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDL 64
           E WRF KQV +GRWF+VFASF+IMIGAGS Y+FGTYSK IK+QFDY+QTQ+NTLGF+KDL
Sbjct: 8   ESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDL 67

Query: 65  GSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAAN 124
           GSN GVFAGLL EVAPPWVLF+VGS+LNF+S+ MIWLS+T RI+KP FW+MF+++ +AAN
Sbjct: 68  GSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAAN 127

Query: 125 AQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLS 184
           +QNFANTAV+V SV+NFPDRRGII+GLLKGFVG GGA+LTQ YLA+YGH++P++++LLLS
Sbjct: 128 SQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLS 187

Query: 185 WFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHAAY 244
           W P+V S++   SIR I+ RK P+EL+V YHLLY SIILA+ +L +T+TQK  +F+  AY
Sbjct: 188 WLPTVASMLFFLSIRPIKVRKDPDELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAY 247

Query: 245 LGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAV--DPSVTLSIPEEKQSLPYSNILLSTV 304
           +    V++  L +PLLIAI+EEF+LFKLN Q +  +P+V + +PE + S P +   +  +
Sbjct: 248 MSGATVIIGLLLIPLLIAIREEFMLFKLNKQTLNNNPTVPILMPEHEPS-PKAPPTIQKI 307

Query: 305 LEKDSP------SKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESL 364
            E  +        KP+RGEDFTILQAL SKDMALVF+AT++ACG+SI AIDNLGQ+ ESL
Sbjct: 308 PEASTSCFSMTFKKPQRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESL 367

Query: 365 AYPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAF 424
            YP+Q+IS+FVSW+SIFNFFGRVFSGFISE LMTKYKLPRPLMFA +HLLTCIGILFIAF
Sbjct: 368 GYPSQTISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAF 427

Query: 425 PYHGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLV 484
           PY GSVY ASLIIG GFGAQVP+LFAIIS+LFGLK+YST+ NCGQLAVP+GSYILNV ++
Sbjct: 428 PYKGSVYAASLIIGFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVI 487

Query: 485 GRMYDMEATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGD 544
           G++YD+EA + G  K+GKGLTC G HCF GSF ILA + LFGA++SL LA RTR+FYKGD
Sbjct: 488 GKLYDIEAAKLGGVKNGKGLTCTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGD 547

Query: 545 IYKRYRDDMWITQSDM 553
           +Y RYR+DMWI QSDM
Sbjct: 548 VYNRYREDMWIPQSDM 562

BLAST of MS006871.1 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.6e-28
Identity = 136/536 (25.37%), Postives = 250/536 (46.64%), Query Frame = 0

Query: 17  RWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNLGVFAGLLA 76
           +W  + A+  I    G+ + F  YS  +K+    SQ ++N L  + DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 77  EVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFANTAVMVI 136
              P  V+    +++ F  + + WL +T+ I  P +  +FL   LA  +  + NTA  ++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 137 SVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSD---VLLLLSWFPSVISIV 196
            +++FP+ R + + L   F G+  A+ +  + AI    +PS     LLL S  P V+S  
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAI----NPSSSNLYLLLNSLVPLVVSFA 222

Query: 197 LLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHAA-------YLG 256
            L+ + T   +   +    +    + S +  I  ++  +T  H++ S ++       ++G
Sbjct: 223 ALYPVLT---KPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIG 282

Query: 257 SVAVVVASLCLPLLIAIKEEFLLF---KLNNQAVDPSVTLSIPEEKQSLPYSNILLSTVL 316
           +V ++V  LC PLL+  ++ FL     +LN+++    V L+I E K     +++   T  
Sbjct: 283 AVVLLVFPLCAPLLVYARDYFLPVINARLNHES-SGYVMLNIDELKNQ--KTSVSSKTGY 342

Query: 317 EKDSPSKP----ERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAYP 376
           E    +K       G++ +    +   +  L ++A        +V  +NLGQIA+SL   
Sbjct: 343 EHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSL--- 402

Query: 377 AQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIG--ILFIAFP 436
            Q+ +  V+  S F+FFGR+ S    + +  +++L R   FA+A L T I   +L ++  
Sbjct: 403 GQNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSS 462

Query: 437 YHGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVG 496
              ++  A+ +IG   G       +I SDLFG        N     +P+GS +L   +  
Sbjct: 463 QQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIAA 522

Query: 497 RMYDMEATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFY 534
            +Y+  A+          + C G  C+  +F     +++ G +SSL+L  RT+  Y
Sbjct: 523 SIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of MS006871.1 vs. ExPASy Swiss-Prot
Match: Q03795 (Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YMR155W PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.4e-08
Identity = 117/530 (22.08%), Postives = 214/530 (40.38%), Query Frame = 0

Query: 26  VIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNL-GVFAGLLAEVAPPWVL 85
           V+ +GAG+PYLF  Y+  + ++     +  + L FS  +GS+L G+ AG++ + +P  + 
Sbjct: 27  VVALGAGTPYLFSFYAPQLLSKCHIPVSASSKLSFSLTIGSSLMGILAGIVVDRSPK-LS 86

Query: 86  FVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFANTAVMVISVK----N 145
            ++GS   F +++++ L   H       W     + L+     + + +    SVK    N
Sbjct: 87  CLIGSMCVFIAYLILNLCYKHE------WSSTFLISLSLVLIGYGSVSGFYASVKCANTN 146

Query: 146 FPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPSVISIVLLFSIRT 205
           FP  RG          GL G V + +   ++G E+   V + L      + +V  FS+  
Sbjct: 147 FPQHRGTAGAFPVSLYGLSGMVFSYLCSKLFG-ENIEHVFIFLMVACGCMILVGYFSL-D 206

Query: 206 IRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHAAYLGSVAVVVASLCLPLL 265
           I +    ++  +    L  S        I+ L +    +  +    S      +  L   
Sbjct: 207 IFSNAEGDDASIKEWELQKS--RETDDNIVPLYENSNDYIGSPVRSSSPATYETYALSDN 266

Query: 266 IAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLSTVLEKDSPSKPERGEDFTIL 325
                EF   +    +  P ++ S P  K      N   +TV E  +    ++     + 
Sbjct: 267 FQETSEFFALEDRQLSNRPLLSPSSPHTKYDFEDENTSKNTVGENSA----QKSMRLHVF 326

Query: 326 QALLSKDMALVFLATVSACGSSIVAIDNLGQIAES---------LAYPAQSI-SVFVSWI 385
           Q+L S      ++      G  ++ I ++G + ++         L   A+ I S+ V+ +
Sbjct: 327 QSLKSSTFIGYYIVLGILQGVGLMYIYSVGFMVQAQVSTPPLNQLPINAEKIQSLQVTLL 386

Query: 386 SIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHG---------- 445
           S+ +F GR+ SG IS+ L+ K+K  R     +A LL  +    I+  +            
Sbjct: 387 SLLSFCGRLSSGPISDFLVKKFKAQRLWNIVIASLLVFLASNKISHDFSSIEDPSLRASK 446

Query: 446 ---SVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVL--L 505
              ++ V S I G  FG       +I++D FG   YSTL     +    G + ++V   +
Sbjct: 447 SFKNISVCSAIFGYSFGVLFGTFPSIVADRFGTNGYSTLWG---VLTTGGVFSVSVFTDI 506

Query: 506 VGRMYDMEATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLAL 526
           +GR  D +A    NT    G    G  C+  +F +      F  +  L +
Sbjct: 507 LGR--DFKA----NTGDDDGNCKKGVLCYSYTFMVTKYCAAFNLLFVLGI 532

BLAST of MS006871.1 vs. ExPASy TrEMBL
Match: A0A6J1DG00 (uncharacterized membrane protein YMR155W-like OS=Momordica charantia OX=3673 GN=LOC111020471 PE=4 SV=1)

HSP 1 Score: 1061.2 bits (2743), Expect = 1.5e-306
Identity = 547/552 (99.09%), Postives = 549/552 (99.46%), Query Frame = 0

Query: 1   MCAAEGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGF 60
           MCAAEGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGF
Sbjct: 4   MCAAEGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGF 63

Query: 61  SKDLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVG 120
           SKDLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVG
Sbjct: 64  SKDLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVG 123

Query: 121 LAANAQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVL 180
           LAANAQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVL
Sbjct: 124 LAANAQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVL 183

Query: 181 LLLSWFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFS 240
           LLLSWFPSVISIVLLFSIRTIRAR HPEELKVFYHLLYASIILAISILIITLTQKHI+FS
Sbjct: 184 LLLSWFPSVISIVLLFSIRTIRARXHPEELKVFYHLLYASIILAISILIITLTQKHIVFS 243

Query: 241 HAAYLGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLS 300
           HAAYLGSVAVVVA LCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLS
Sbjct: 244 HAAYLGSVAVVVALLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLS 303

Query: 301 TVLEKDSPSKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAYPA 360
           TVLEKDSPSKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAYPA
Sbjct: 304 TVLEKDSPSKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAYPA 363

Query: 361 QSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHG 420
           QSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHG
Sbjct: 364 QSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHG 423

Query: 421 SVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVGRMY 480
           SVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVGRMY
Sbjct: 424 SVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVGRMY 483

Query: 481 DMEATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGDIYKR 540
           DMEA RAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGDIYKR
Sbjct: 484 DMEANRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGDIYKR 543

Query: 541 YRDDMWITQSDM 553
           YRDDMWIT+SDM
Sbjct: 544 YRDDMWITESDM 555

BLAST of MS006871.1 vs. ExPASy TrEMBL
Match: A0A6J1DHB0 (uncharacterized protein LOC111020473 OS=Momordica charantia OX=3673 GN=LOC111020473 PE=4 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 4.6e-228
Identity = 406/550 (73.82%), Postives = 464/550 (84.36%), Query Frame = 0

Query: 3   AAEGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSK 62
           AA  W F KQVA+GRWFSVFASF+IMIGAGS YLFGTYSK++KTQFDY QTQVNTLGF+K
Sbjct: 7   AAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGFAK 66

Query: 63  DLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLA 122
           DLGSN GVFAGLLAEVAP WVLF+VGSSLNFFS+ MIWLSVT RIA P+FW M LY+ LA
Sbjct: 67  DLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYICLA 126

Query: 123 ANAQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLL 182
           AN+QNFANTAV+V SVKNFP+RRGII+GLLKGFVG+GGA+LTQ+YLAIYGHE+ + V+LL
Sbjct: 127 ANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENQASVILL 186

Query: 183 LSWFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHA 242
           L+WFPSVIS V + SIRTI+ RKHPEELKVFYHLLY SIILA+ IL +TLTQK  +FSHA
Sbjct: 187 LAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVFSHA 246

Query: 243 AYLGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLSTV 302
            Y+G  +V+V  LCLPLLIAI+EEF+LF LN Q  D  V + I ++K   P    L    
Sbjct: 247 GYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKP--PPKRALEEDA 306

Query: 303 LEKDSPSKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAYPAQS 362
           ++  S  KPERG+DFTI QALLSKDMAL+F+AT+S CGSSI AIDN+GQIAESL YP  S
Sbjct: 307 IDSVS-IKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQYPPHS 366

Query: 363 ISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSV 422
           I++FVSW+SIFNFFGRVFSGFISETLM KYKLPRPLMFA + LLTCIG L IAFPY GSV
Sbjct: 367 IAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPYRGSV 426

Query: 423 YVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVGRMYDM 482
           Y ASLIIG GFGAQVPLLFAIIS+LFGLKHYSTL NCGQLAVP GSYILNV ++G++YDM
Sbjct: 427 YAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGKLYDM 486

Query: 483 EATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGDIYKRYR 542
           EA R G  K GKGLTC G +CF  SFTILAA+T FGA+SSL LA RTR+FYKG+ YK+YR
Sbjct: 487 EAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFFGALSSLVLAYRTRDFYKGETYKKYR 546

Query: 543 DDMWITQSDM 553
           +DMWI ++DM
Sbjct: 547 EDMWIPKADM 553

BLAST of MS006871.1 vs. ExPASy TrEMBL
Match: A0A6J1HYK5 (uncharacterized protein LOC111469251 OS=Cucurbita maxima OX=3661 GN=LOC111469251 PE=4 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 4.2e-221
Identity = 378/556 (67.99%), Postives = 468/556 (84.17%), Query Frame = 0

Query: 5   EGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDL 64
           E WRF KQV +GRWF+VFASF+IMIGAGS Y+FGTYSK IK+QFDY+QTQ+NTLGF+KDL
Sbjct: 8   ESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDL 67

Query: 65  GSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAAN 124
           GSN GVFAGLL EVAPPWVLF+VGS+LNF+S+ MIWLS+T RI KP FW+MF+++ +AAN
Sbjct: 68  GSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRIVKPPFWEMFVFICVAAN 127

Query: 125 AQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLS 184
           +QNFANTA++V SV+NFPDRRGII+GLLKGFVG GGA+LTQ YLA+YGH++P++++LLLS
Sbjct: 128 SQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLS 187

Query: 185 WFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHAAY 244
           W P+V+S++   SIR I+ RKHP+EL+V YHLLY SIILA+ +L +T+TQK  +F+  AY
Sbjct: 188 WLPTVVSMLFFLSIRPIKVRKHPDELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAY 247

Query: 245 LGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAV--DPSVTLSIPEEKQSLPYSNILLSTV 304
           +    V++  L +PLLIAI+EEFLLFKLN Q +  +P+V + +PE + S P +   +  +
Sbjct: 248 MSGATVIIGLLLIPLLIAIREEFLLFKLNKQTLNNNPTVPILMPEHEPS-PKAPPTIEQI 307

Query: 305 LEKDSP------SKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESL 364
            E  +        +P+RGEDFTILQAL SKDMALVF+AT+SACG+SI AIDNLGQ+ ESL
Sbjct: 308 PEASTSCFSMAFKRPQRGEDFTILQALFSKDMALVFIATLSACGASIAAIDNLGQVGESL 367

Query: 365 AYPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAF 424
            YP+Q+IS+FVSW+SIFNFFGRVFSGFIS+ LMTKYKLPRPLMFA +H+LTC+GILFIAF
Sbjct: 368 GYPSQTISIFVSWVSIFNFFGRVFSGFISDALMTKYKLPRPLMFAFSHILTCVGILFIAF 427

Query: 425 PYHGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLV 484
           PY GSVY ASLIIG GFGAQVP+LFAIIS+LFGLK+YST+ NCGQLAVP+GSYILNV ++
Sbjct: 428 PYKGSVYAASLIIGFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVI 487

Query: 485 GRMYDMEATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGD 544
           GR+YD+EA + G  K+GKGLTC G  CF GSF ILA + LFGA++SL LA RTR+FYKGD
Sbjct: 488 GRLYDIEAAKLGGVKNGKGLTCTGTPCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGD 547

Query: 545 IYKRYRDDMWITQSDM 553
           +Y RYR+DMWI QSDM
Sbjct: 548 VYNRYREDMWIPQSDM 562

BLAST of MS006871.1 vs. ExPASy TrEMBL
Match: A0A6J1HIM6 (uncharacterized protein LOC111464777 OS=Cucurbita moschata OX=3662 GN=LOC111464777 PE=4 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 1.2e-220
Identity = 379/556 (68.17%), Postives = 467/556 (83.99%), Query Frame = 0

Query: 5   EGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDL 64
           E WRF KQV +GRWF+VFASF+IMIGAGS Y+FGTYSK IK+QFDY+QTQ+NTLGF+KDL
Sbjct: 8   ESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDL 67

Query: 65  GSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAAN 124
           GSN GVFAGLL EVAPPWVLF+VGS+LNF+S+ MIWLS+T RI+KP FW+MF+++ +AAN
Sbjct: 68  GSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAAN 127

Query: 125 AQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLS 184
           +QNFANTA++V SV+NFPDRRGII+GLLKGFVG GGA+LTQ YLA+YGH++P++++LLLS
Sbjct: 128 SQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLS 187

Query: 185 WFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHAAY 244
           W P+V S++   SIR I+ RK P+EL+V YHLLY SIILA+ +L +T+TQK  +F+  AY
Sbjct: 188 WLPTVASMLFFLSIRPIKVRKDPDELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAY 247

Query: 245 LGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAV--DPSVTLSIPEEKQSLPYSNILLSTV 304
           +    V++  L +PLLIAI+EEF+LFKLN Q +  +P+V + +PE + S P +   +  +
Sbjct: 248 MSGATVIIGLLLIPLLIAIREEFMLFKLNKQTLNNNPTVPILMPEHEPS-PKAPPTIQQI 307

Query: 305 LEKDSP------SKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESL 364
            E  +        KP+RGEDFTILQAL SKDMALVF+AT++ACG+SI AIDNLGQ+ ESL
Sbjct: 308 PEASTSCFSMTFKKPQRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESL 367

Query: 365 AYPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAF 424
            YP Q+IS+FVSW+SIFNFFGRVFSGFISE LMTKYKLPRPLMFA +HLLTCIGILFIAF
Sbjct: 368 GYPYQTISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAF 427

Query: 425 PYHGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLV 484
           PY GSVY ASLIIG GFGAQVP+LFAIIS+LFGLK+YST+ NCGQLAVP+GSYILNV ++
Sbjct: 428 PYKGSVYAASLIIGFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVI 487

Query: 485 GRMYDMEATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGD 544
           G++YD+EA + G  K+GKGLTC G HCF GSF ILA + LFGA++SL LA RTR+FYKGD
Sbjct: 488 GKLYDIEAAKLGGVKNGKGLTCTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGD 547

Query: 545 IYKRYRDDMWITQSDM 553
           +Y RYR+DMWI QSDM
Sbjct: 548 VYNRYREDMWIPQSDM 562

BLAST of MS006871.1 vs. ExPASy TrEMBL
Match: A0A6J1I3B9 (uncharacterized protein LOC111469246 OS=Cucurbita maxima OX=3661 GN=LOC111469246 PE=4 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.6e-220
Identity = 379/556 (68.17%), Postives = 468/556 (84.17%), Query Frame = 0

Query: 5   EGWRFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDL 64
           E WRF KQV +GRWF+VFASF+IMIGAGS Y+FGTYSK IK+QF+Y+QTQ+NTLGF+KDL
Sbjct: 8   ESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFNYNQTQINTLGFAKDL 67

Query: 65  GSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAAN 124
           GSN GVFAGLL EVAPPWVLF+VGS+LNF+S+ MIWLS+T RIAKP FW+MFL++ +AAN
Sbjct: 68  GSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRIAKPPFWEMFLFICVAAN 127

Query: 125 AQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLS 184
           +QNFANTA++V SV+NFPDRRGII+GLLKGFVG GGA+LTQ YLA+YGH++P++++LLLS
Sbjct: 128 SQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLS 187

Query: 185 WFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHAAY 244
           W P+V+S++   SIR I+ RKHP+EL+V YHLLY SIILA+ +L +T+TQK  +F+  AY
Sbjct: 188 WLPTVVSMLFFLSIRPIKVRKHPDELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAY 247

Query: 245 LGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAV--DPSVTLSIPEEKQSLPYSNILLSTV 304
           +    V++  L +PLLIAI+EEFLLFKLN Q +  +P+V + +PE + S P +   +  +
Sbjct: 248 MSGATVIIGLLLIPLLIAIREEFLLFKLNKQTLNNNPTVPILMPEHEPS-PKAPPTIEQI 307

Query: 305 LEKDSP------SKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESL 364
            E  +        +P+RGEDFTILQAL SKDMALVF+AT+SACG+SI AIDNLGQ+ ESL
Sbjct: 308 PEASTSCFSMAFKRPQRGEDFTILQALFSKDMALVFIATLSACGASIAAIDNLGQVGESL 367

Query: 365 AYPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAF 424
            YP+Q+IS+FVSW+SIFNFFGRVFSGFISE LMTKYKLPRPLMFA +HLLTCIGILFIAF
Sbjct: 368 GYPSQTISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAF 427

Query: 425 PYHGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLV 484
           PY GSVY ASLIIG GFGAQVP++FAIIS+LFGLK+YST+ NCGQLAVP+GSYILNV ++
Sbjct: 428 PYKGSVYAASLIIGFGFGAQVPMVFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVI 487

Query: 485 GRMYDMEATRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGD 544
           G++YD+EA + G  K+GKGLTC G  CF GSF ILA + LFGA+ SL LA RT++FYKGD
Sbjct: 488 GKLYDIEAAKLGGVKNGKGLTCTGTPCFSGSFLILAVVVLFGAVVSLVLAYRTQDFYKGD 547

Query: 545 IYKRYRDDMWITQSDM 553
           +Y RYR+DMWI QSDM
Sbjct: 548 VYNRYREDMWIPQSDM 562

BLAST of MS006871.1 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 551.2 bits (1419), Expect = 9.4e-157
Identity = 286/550 (52.00%), Postives = 381/550 (69.27%), Query Frame = 0

Query: 8   RFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSN 67
           RF     +GRWF VFASF+IM  AG+ YLFGTYSK IK+   Y QT +N LGF KDLG+N
Sbjct: 8   RFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGAN 67

Query: 68  LGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQN 127
           +GV +GL+AEV P W +  +GS++NF  + MIWL+VT ++AKP+ WQM LY+ + AN+QN
Sbjct: 68  VGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQN 127

Query: 128 FANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFP 187
           FANT  +V  VKNFP+ RG+++GLLKG+VGL GA+ TQ+Y AIYGH D   ++LL++W P
Sbjct: 128 FANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGH-DSKSLILLIAWLP 187

Query: 188 SVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHAAYLGS 247
           + +S+V ++ IR  +  +   EL VFY  LY SI LA+ ++ + + +K + FS AAY  S
Sbjct: 188 AAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAAS 247

Query: 248 VAVVVASLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLSTVLEKDS 307
             +  A L +PL +++K+E  ++ +    ++    + + + K+ L       + V  ++ 
Sbjct: 248 ATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEK 307

Query: 308 PSK---------PERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAY 367
            +K         P RGED+TILQALLS DM ++F+AT    GSS+ A+DNLGQI ESL Y
Sbjct: 308 ETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGY 367

Query: 368 PAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPY 427
           P  ++S FVS +SI+N+FGRVFSGF+SE L+ KYKLPRPLM  L  LL+C G L IAFP 
Sbjct: 368 PNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPV 427

Query: 428 HGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVGR 487
            GSVY+AS+++G  FGAQ+PLLFAIIS+LFGLK+YSTL NCGQLA PLGSYILNV + G 
Sbjct: 428 PGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGM 487

Query: 488 MYDMEA-----TRAGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFY 544
           +YD EA      R    K  K LTC G  C+   F ILAA+T FGA+ SL LA RTR FY
Sbjct: 488 LYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFY 547

BLAST of MS006871.1 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 506.1 bits (1302), Expect = 3.5e-143
Identity = 269/560 (48.04%), Postives = 374/560 (66.79%), Query Frame = 0

Query: 12  QVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNLGVF 71
           Q+  GRWF  F S +IM  AG+ Y+FG YS  IK    Y QT +N L F KDLG+N+GV 
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 72  AGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFANT 131
           AGLL EV PPW + ++G+ LNFF + MIWL+VT RI+KP+ W M LY+ + AN+Q+FANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 132 AVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPSVIS 191
             +V  VKNFP+ RG+++G+LKG+VGL GA++TQ+Y A YG ED  +++L++ W P+++S
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAIVS 195

Query: 192 IVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFSHAAYLGSVAVV 251
              L +IR ++ ++   ELKVFY+ LY S+ LA  ++++ +  K   F+ + + GS AVV
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 255

Query: 252 VASLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNIL------LSTVLEK 311
           +  L LP+++ I EE  L+K    A++    +++  EK  L  S            V+EK
Sbjct: 256 IVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEK 315

Query: 312 -DSPS-------KPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLA 371
             +PS        PERG+D+TILQAL S DM ++FLAT+   G ++ AIDNLGQI  SL 
Sbjct: 316 VKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLG 375

Query: 372 YPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFP 431
           YP +S+S FVS +SI+N++GRV SG +SE  + KYK PRPLM  +  LL+C G L IAF 
Sbjct: 376 YPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFN 435

Query: 432 YHGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVG 491
             G +YVAS+IIG  FGAQ PLLFAIIS++FGLK+YSTL N G +A P+GSY+LNV + G
Sbjct: 436 VPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAG 495

Query: 492 RMYDMEATR----AGNTK-HGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNF 551
            +YD+EA +     G T+  G+ L C G  CF  SF I+AA+TLFG + S+ L  RT+ F
Sbjct: 496 YLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKF 555

Query: 552 YKGDIYKRYRDDMWITQSDM 553
           YK DIYK++R+     + +M
Sbjct: 556 YKSDIYKKFREKALAAEMEM 574

BLAST of MS006871.1 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 7.1e-80
Identity = 183/538 (34.01%), Postives = 300/538 (55.76%), Query Frame = 0

Query: 7   WRFAKQ-VADGRWFSVFASFVIMIGAGSPYLF-GTYSKVIKTQFDYSQTQVNTLGFSKDL 66
           WR   + + + RW     +  +   AG  YLF G+ S  IKT   Y+Q Q+  LG +K+L
Sbjct: 5   WRHKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNL 64

Query: 67  GSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAAN 126
           G  +G  +G L+EV+P WV+ +VG++ N F + ++WL VT ++     W +F+ + +  N
Sbjct: 65  GDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTN 124

Query: 127 AQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLS 186
            + + NTA +V  + NFP+ RG ++G+LKGF GL GA+LTQ+YL ++     S V+L+++
Sbjct: 125 GETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVA 184

Query: 187 WFPSVISIVLLFSIRTI----RARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFS 246
             P V+ + LLF +R +    R     ++L+ F  +    ++LA+ +L + + Q     +
Sbjct: 185 LGPPVVVLALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMT 244

Query: 247 HAAYLGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLS 306
                 S A++V  + +P+L+     F+    NN      VT   PEE  S    +   +
Sbjct: 245 QTIITTSGAILVIFMVVPVLVPFSSVFI--SGNN------VTSVKPEEGTSNVDQHEART 304

Query: 307 TVLEKDSPSKPER----GEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESL 366
            +   D P + +R    GEDFT+LQAL   D  L+F++ V   GS I  IDNLGQI  SL
Sbjct: 305 LIERSDRPPEKKRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSL 364

Query: 367 AYPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAF 426
            Y   +  +FVS ISI NF GRV  G+ SE ++ K  LPR L  ++   +  +G+++ A 
Sbjct: 365 GY--SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAI 424

Query: 427 PYHGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLV 486
            + G +YV +++IG G+GA   +  A +SD+FGLK + +L N    A+P+GS++ + ++ 
Sbjct: 425 DWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIA 484

Query: 487 GRMYDMEATR-AGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFY 534
             +YD  A + AG T   + L C G  C+  + ++++ + L   + SL++  RTR FY
Sbjct: 485 SNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of MS006871.1 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 7.1e-80
Identity = 183/538 (34.01%), Postives = 300/538 (55.76%), Query Frame = 0

Query: 7   WRFAKQ-VADGRWFSVFASFVIMIGAGSPYLF-GTYSKVIKTQFDYSQTQVNTLGFSKDL 66
           WR   + + + RW     +  +   AG  YLF G+ S  IKT   Y+Q Q+  LG +K+L
Sbjct: 5   WRHKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNL 64

Query: 67  GSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAAN 126
           G  +G  +G L+EV+P WV+ +VG++ N F + ++WL VT ++     W +F+ + +  N
Sbjct: 65  GDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTN 124

Query: 127 AQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLS 186
            + + NTA +V  + NFP+ RG ++G+LKGF GL GA+LTQ+YL ++     S V+L+++
Sbjct: 125 GETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVA 184

Query: 187 WFPSVISIVLLFSIRTI----RARKHPEELKVFYHLLYASIILAISILIITLTQKHIIFS 246
             P V+ + LLF +R +    R     ++L+ F  +    ++LA+ +L + + Q     +
Sbjct: 185 LGPPVVVLALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMT 244

Query: 247 HAAYLGSVAVVVASLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLS 306
                 S A++V  + +P+L+     F+    NN      VT   PEE  S    +   +
Sbjct: 245 QTIITTSGAILVIFMVVPVLVPFSSVFI--SGNN------VTSVKPEEGTSNVDQHEART 304

Query: 307 TVLEKDSPSKPER----GEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESL 366
            +   D P + +R    GEDFT+LQAL   D  L+F++ V   GS I  IDNLGQI  SL
Sbjct: 305 LIERSDRPPEKKRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSL 364

Query: 367 AYPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAF 426
            Y   +  +FVS ISI NF GRV  G+ SE ++ K  LPR L  ++   +  +G+++ A 
Sbjct: 365 GY--SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAI 424

Query: 427 PYHGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLV 486
            + G +YV +++IG G+GA   +  A +SD+FGLK + +L N    A+P+GS++ + ++ 
Sbjct: 425 DWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIA 484

Query: 487 GRMYDMEATR-AGNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFY 534
             +YD  A + AG T   + L C G  C+  + ++++ + L   + SL++  RTR FY
Sbjct: 485 SNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of MS006871.1 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 1.5e-77
Identity = 185/566 (32.69%), Postives = 295/566 (52.12%), Query Frame = 0

Query: 15  DGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNLGVFAGL 74
           + RW    A+  I   AG  YLFG+ S VIK+  +Y+Q +++ LG +KDLG ++G  AG 
Sbjct: 14  NNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGT 73

Query: 75  LAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFANTAVM 134
           L+E+ P W   +VG+  N   +  +WL VT R      W M + + +  N + + NT  +
Sbjct: 74  LSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGAL 133

Query: 135 VISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPSVISIVL 194
           V  V+NFP  RG ++G+LKGF GLGGA+++QIY  I+   +P+ ++L+++  P+V+ + L
Sbjct: 134 VSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHS-SNPASLILMVAVTPAVVVVCL 193

Query: 195 LFSIRTIRARK--HPEELKVFYHLLYASIILAISILIITLTQKHIIFSHAAYLGSVAVVV 254
           +F IR +   K   P +   F  +    ++LA  ++ + L Q  ++ SH        V+ 
Sbjct: 194 MFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLF 253

Query: 255 ASLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIP---EEKQSLPYSNILLSTVLEKDSPS 314
             L +P+L+ I   F  F   N+  D      +P   +++  L   +++LS V E + P 
Sbjct: 254 VILVVPILVPIMTSF--FTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEV-EDEKPK 313

Query: 315 K------------------------------------PERGEDFTILQALLSKDMALVFL 374
                                                P RGEDFT+ QAL+  D  L+F 
Sbjct: 314 DVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFF 373

Query: 375 ATVSACGSSIVAIDNLGQIAESLAYPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYK 434
           + +   GS +  IDNLGQ+++SL Y   +  V VS ISI+NF GR+  G+ SE ++  Y 
Sbjct: 374 SLLLGSGSGLTVIDNLGQMSQSLGY--DNTHVLVSMISIWNFLGRIGGGYFSELVVRDYA 433

Query: 435 LPRPLMFALAHLLTCIGILFIAFPYHGSVYVASLIIGSGFGAQVPLLFAIISDLFGLKHY 494
            PRP+  A+A L+  +G +F A+ + G++Y+ +L+IG G+GA   ++ A  S+LFGLK +
Sbjct: 434 YPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKF 493

Query: 495 STLLNCGQLAVPLGSYILNVLLVGRMYDMEATRAGNTKHGK------GLTCNGPHCFGGS 534
             L N   LA P GS + + ++   +YD EA R     HG        L CNG  CF  +
Sbjct: 494 GALYNFLTLANPAGSLVFSGMIASSIYDREAERQA---HGSVFDPDDALRCNGSICFFLT 553

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152848.13.0e-30699.09uncharacterized membrane protein YMR155W-like [Momordica charantia][more]
XP_022152849.19.5e-22873.82uncharacterized protein LOC111020473 [Momordica charantia][more]
XP_038895745.14.6e-22270.68uncharacterized protein LOC120083909 [Benincasa hispida][more]
XP_022970242.18.7e-22167.99uncharacterized protein LOC111469251 [Cucurbita maxima][more]
KAG6583549.18.7e-22168.35Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
F4I9E11.6e-2825.37Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q037952.4e-0822.08Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATC... [more]
Match NameE-valueIdentityDescription
A0A6J1DG001.5e-30699.09uncharacterized membrane protein YMR155W-like OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1DHB04.6e-22873.82uncharacterized protein LOC111020473 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1HYK54.2e-22167.99uncharacterized protein LOC111469251 OS=Cucurbita maxima OX=3661 GN=LOC111469251... [more]
A0A6J1HIM61.2e-22068.17uncharacterized protein LOC111464777 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
A0A6J1I3B91.6e-22068.17uncharacterized protein LOC111469246 OS=Cucurbita maxima OX=3661 GN=LOC111469246... [more]
Match NameE-valueIdentityDescription
AT2G28120.19.4e-15752.00Major facilitator superfamily protein [more]
AT2G39210.13.5e-14348.04Major facilitator superfamily protein [more]
AT5G50630.17.1e-8034.01Major facilitator superfamily protein [more]
AT5G50520.17.1e-8034.01Major facilitator superfamily protein [more]
AT5G14120.11.5e-7732.69Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 327..531
e-value: 1.7E-7
score: 32.8
coord: 16..213
e-value: 3.7E-7
score: 31.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 17..531
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 17..263
e-value: 4.6E-74
score: 249.1
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 4..544
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 4..544
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 16..519
e-value: 4.83926E-93
score: 287.996

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MS006871MS006871gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MS006871.1-cdsMS006871.1-cds-scaffold871:300563..302218CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MS006871.1MS006871.1-proteinpolypeptide