MS004933.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS004933.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Eukaryotic translation initiation factor 4C
Location	scaffold176: 1493926 .. 1494360 (+)
Sequence length	435
RNA-Seq Expression	MS004933.1
Synteny	MS004933.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAAAGAATAAGGGAAAGGGAGGAAAGAACAGGAAGAGAGGAAAGAATGAAGCAGATGATGAGAAGCGTGAACTTGTGTTCAAGGAAGATGGTCAGGAGTATGCACAAGTTCTCAGAATGTTGGGAAATGGTCGTTGTGAGGCAATGTGCATAGATGGGACAAAGCGCCTTTGCCATATTCGGGGGAAGATGCACAAGAAGGTTTGGATAGCGGCTGGTGATATTATACTTGTTGGTCTTCGCGACTATCAGGATGACAAAGCTGATGTTATCCTCAAGTATATGCCTGATGAAGCAAGGCTGTTGAAGGCATATGGTGAGCTTCCTGAGAGCACACGTCTGAACGAGGGTATTGCTGGTGGTATCGACGATGAGGATGATGCTGGGGGTGATGATTATCTTGAGTTTGAGGATGAAGATATTGATAAGATT

mRNA sequence

ATGCCAAAGAATAAGGGAAAGGGAGGAAAGAACAGGAAGAGAGGAAAGAATGAAGCAGATGATGAGAAGCGTGAACTTGTGTTCAAGGAAGATGGTCAGGAGTATGCACAAGTTCTCAGAATGTTGGGAAATGGTCGTTGTGAGGCAATGTGCATAGATGGGACAAAGCGCCTTTGCCATATTCGGGGGAAGATGCACAAGAAGGTTTGGATAGCGGCTGGTGATATTATACTTGTTGGTCTTCGCGACTATCAGGATGACAAAGCTGATGTTATCCTCAAGTATATGCCTGATGAAGCAAGGCTGTTGAAGGCATATGGTGAGCTTCCTGAGAGCACACGTCTGAACGAGGGTATTGCTGGTGGTATCGACGATGAGGATGATGCTGGGGGTGATGATTATCTTGAGTTTGAGGATGAAGATATTGATAAGATT

Coding sequence (CDS)

ATGCCAAAGAATAAGGGAAAGGGAGGAAAGAACAGGAAGAGAGGAAAGAATGAAGCAGATGATGAGAAGCGTGAACTTGTGTTCAAGGAAGATGGTCAGGAGTATGCACAAGTTCTCAGAATGTTGGGAAATGGTCGTTGTGAGGCAATGTGCATAGATGGGACAAAGCGCCTTTGCCATATTCGGGGGAAGATGCACAAGAAGGTTTGGATAGCGGCTGGTGATATTATACTTGTTGGTCTTCGCGACTATCAGGATGACAAAGCTGATGTTATCCTCAAGTATATGCCTGATGAAGCAAGGCTGTTGAAGGCATATGGTGAGCTTCCTGAGAGCACACGTCTGAACGAGGGTATTGCTGGTGGTATCGACGATGAGGATGATGCTGGGGGTGATGATTATCTTGAGTTTGAGGATGAAGATATTGATAAGATT

Protein sequence

MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIAGGIDDEDDAGGDDYLEFEDEDIDKI

Homology

[Close]

BLAST of MS004933.1 vs. NCBI nr
Match: XP_022140781.1 (eukaryotic translation initiation factor 1A-like [Momordica charantia])

HSP 1 Score: 290.8 bits (743), Expect = 6.5e-75
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDDAGGDDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 145

[Close]

BLAST of MS004933.1 vs. NCBI nr
Match: XP_008457652.1 (PREDICTED: eukaryotic translation initiation factor 1A [Cucumis melo] >XP_008457653.1 PREDICTED: eukaryotic translation initiation factor 1A [Cucumis melo] >KAA0045698.1 eukaryotic translation initiation factor 1A [Cucumis melo var. makuwa])

HSP 1 Score: 289.3 bits (739), Expect = 1.9e-74
Identity = 144/145 (99.31%), Postives = 144/145 (99.31%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD GGDDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDGGGDDYLEFEDEDIDKI 145

[Close]

BLAST of MS004933.1 vs. NCBI nr
Match: XP_022145199.1 (eukaryotic translation initiation factor 1A [Momordica charantia] >XP_022956094.1 eukaryotic translation initiation factor 1A [Cucurbita moschata] >XP_022956095.1 eukaryotic translation initiation factor 1A [Cucurbita moschata] >XP_022979975.1 eukaryotic translation initiation factor 1A [Cucurbita maxima] >XP_022979976.1 eukaryotic translation initiation factor 1A [Cucurbita maxima] >XP_023527417.1 eukaryotic translation initiation factor 1A [Cucurbita pepo subsp. pepo] >XP_023527418.1 eukaryotic translation initiation factor 1A [Cucurbita pepo subsp. pepo] >XP_038902096.1 eukaryotic translation initiation factor 1A [Benincasa hispida] >XP_038902102.1 eukaryotic translation initiation factor 1A [Benincasa hispida])

HSP 1 Score: 287.0 bits (733), Expect = 9.3e-74
Identity = 143/145 (98.62%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD G DDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDGGADDYLEFEDEDIDKI 145

[Close]

BLAST of MS004933.1 vs. NCBI nr
Match: XP_004147021.1 (eukaryotic translation initiation factor 1A [Cucumis sativus] >XP_011649283.1 eukaryotic translation initiation factor 1A [Cucumis sativus] >KGN61900.1 hypothetical protein Csa_006324 [Cucumis sativus])

HSP 1 Score: 284.3 bits (726), Expect = 6.0e-73
Identity = 141/145 (97.24%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD  GD+Y+EFEDEDIDKI
Sbjct: 121 GGIDDEDDGAGDNYIEFEDEDIDKI 145

[Close]

BLAST of MS004933.1 vs. NCBI nr
Match: CAA2961420.1 (eukaryotic translation initiation factor 1A [Olea europaea subsp. europaea])

HSP 1 Score: 283.9 bits (725), Expect = 7.9e-73
Identity = 140/145 (96.55%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEA CIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEATCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GG+D+EDD GGDDY+EFEDEDIDKI
Sbjct: 121 GGLDEEDDGGGDDYIEFEDEDIDKI 145

[Close]

BLAST of MS004933.1 vs. ExPASy Swiss-Prot
Match: P56331 (Eukaryotic translation initiation factor 1A OS=Onobrychis viciifolia OX=3882 PE=2 SV=2)

HSP 1 Score: 260.8 bits (665), Expect = 9.4e-69
Identity = 133/146 (91.10%), Postives = 140/146 (95.89%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADD+KRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDDKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGI- 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILK MPDEARLLKAYGELP++TRLNEGI 
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKLMPDEARLLKAYGELPDNTRLNEGIG 120

Query: 121 AGGIDDEDDAGGDDYLEFEDEDIDKI 146
           AGG+D+E D   +DY+EFEDEDIDKI
Sbjct: 121 AGGLDEEMDT-ANDYIEFEDEDIDKI 145

[Close]

BLAST of MS004933.1 vs. ExPASy Swiss-Prot
Match: P47815 (Eukaryotic translation initiation factor 1A OS=Triticum aestivum OX=4565 PE=1 SV=2)

HSP 1 Score: 246.1 bits (627), Expect = 2.4e-64
Identity = 123/146 (84.25%), Postives = 135/146 (92.47%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADD+KRELVFKEDGQEYAQV RMLGNGRCEA+C+DGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDDKRELVFKEDGQEYAQVTRMLGNGRCEAICVDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDI+LVGLRDYQDDKADVILKYM DEARLLKAYGELP++ RLNEG+ 
Sbjct: 61  IRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYMNDEARLLKAYGELPDTVRLNEGV- 120

Query: 121 GGIDDEDDAGGD-DYLEFEDEDIDKI 146
             +D  ++  GD DY++FEDEDIDKI
Sbjct: 121 -DVDGPEEGEGDSDYIQFEDEDIDKI 144

[Close]

BLAST of MS004933.1 vs. ExPASy Swiss-Prot
Match: P47813 (Eukaryotic translation initiation factor 1A, X-chromosomal OS=Homo sapiens OX=9606 GN=EIF1AX PE=1 SV=2)

HSP 1 Score: 202.6 bits (514), Expect = 3.0e-51
Identity = 106/145 (73.10%), Postives = 118/145 (81.38%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNR+RGKNE + EKRELVFKEDGQEYAQV++MLGNGR EAMC DG KRLCH
Sbjct: 1   MPKNKGKGGKNRRRGKNENESEKRELVFKEDGQEYAQVIKMLGNGRLEAMCFDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGK+ KKVWI   DIILVGLRDYQD+KADVILKY  DEAR LKAYGELPE  ++NE   
Sbjct: 61  IRGKLRKKVWINTSDIILVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDT 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
            G  D+D+   DD +  +DEDID I
Sbjct: 121 FGPGDDDEIQFDD-IGDDDEDIDDI 144

[Close]

BLAST of MS004933.1 vs. ExPASy Swiss-Prot
Match: Q8BMJ3 (Eukaryotic translation initiation factor 1A, X-chromosomal OS=Mus musculus OX=10090 GN=Eif1ax PE=2 SV=3)

HSP 1 Score: 202.6 bits (514), Expect = 3.0e-51
Identity = 106/145 (73.10%), Postives = 118/145 (81.38%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNR+RGKNE + EKRELVFKEDGQEYAQV++MLGNGR EAMC DG KRLCH
Sbjct: 1   MPKNKGKGGKNRRRGKNENESEKRELVFKEDGQEYAQVIKMLGNGRLEAMCFDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGK+ KKVWI   DIILVGLRDYQD+KADVILKY  DEAR LKAYGELPE  ++NE   
Sbjct: 61  IRGKLRKKVWINTSDIILVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDT 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
            G  D+D+   DD +  +DEDID I
Sbjct: 121 FGPGDDDEIQFDD-IGDDDEDIDDI 144

[Close]

BLAST of MS004933.1 vs. ExPASy Swiss-Prot
Match: Q5RA42 (Eukaryotic translation initiation factor 1A, X-chromosomal OS=Pongo abelii OX=9601 GN=EIF1AX PE=2 SV=3)

HSP 1 Score: 202.6 bits (514), Expect = 3.0e-51
Identity = 106/145 (73.10%), Postives = 118/145 (81.38%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNR+RGKNE + EKRELVFKEDGQEYAQV++MLGNGR EAMC DG KRLCH
Sbjct: 1   MPKNKGKGGKNRRRGKNENESEKRELVFKEDGQEYAQVIKMLGNGRLEAMCFDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGK+ KKVWI   DIILVGLRDYQD+KADVILKY  DEAR LKAYGELPE  ++NE   
Sbjct: 61  IRGKLRKKVWINTSDIILVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDT 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
            G  D+D+   DD +  +DEDID I
Sbjct: 121 FGPGDDDEIQFDD-IGDDDEDIDDI 144

[Close]

BLAST of MS004933.1 vs. ExPASy TrEMBL
Match: A0A6J1CH30 (Eukaryotic translation initiation factor 4C OS=Momordica charantia OX=3673 GN=LOC111011361 PE=3 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 3.1e-75
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDDAGGDDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 145

[Close]

BLAST of MS004933.1 vs. ExPASy TrEMBL
Match: A0A1S3C6N4 (Eukaryotic translation initiation factor 4C OS=Cucumis melo OX=3656 GN=LOC103497302 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 9.1e-75
Identity = 144/145 (99.31%), Postives = 144/145 (99.31%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD GGDDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDGGGDDYLEFEDEDIDKI 145

[Close]

BLAST of MS004933.1 vs. ExPASy TrEMBL
Match: A0A5A7TS70 (Eukaryotic translation initiation factor 4C OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G002160 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 9.1e-75
Identity = 144/145 (99.31%), Postives = 144/145 (99.31%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD GGDDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDGGGDDYLEFEDEDIDKI 145

[Close]

BLAST of MS004933.1 vs. ExPASy TrEMBL
Match: A0A6J1IS99 (Eukaryotic translation initiation factor 4C OS=Cucurbita maxima OX=3661 GN=LOC111479507 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.5e-74
Identity = 143/145 (98.62%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD G DDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDGGADDYLEFEDEDIDKI 145

[Close]

BLAST of MS004933.1 vs. ExPASy TrEMBL
Match: A0A6J1GY26 (Eukaryotic translation initiation factor 4C OS=Cucurbita moschata OX=3662 GN=LOC111457892 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.5e-74
Identity = 143/145 (98.62%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD G DDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDGGADDYLEFEDEDIDKI 145

[Close]

BLAST of MS004933.1 vs. TAIR 10
Match: AT2G04520.1 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 268.5 bits (685), Expect = 3.2e-72
Identity = 131/145 (90.34%), Postives = 139/145 (95.86%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKREL+FKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELIFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDI+LVGLRDYQDDKADVILKYM DEARLLKAYGELPE+TRLNEGI 
Sbjct: 61  IRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEGIV 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           G ++D+DD   DDY+EFEDEDID+I
Sbjct: 121 GDLEDDDDNNDDDYVEFEDEDIDRI 145

[Close]

BLAST of MS004933.1 vs. TAIR 10
Match: AT5G35680.1 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 266.9 bits (681), Expect = 9.3e-72
Identity = 130/145 (89.66%), Postives = 138/145 (95.17%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKREL+FKEDGQEYAQVLRMLGNGRC+ MCIDG KRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYM DEARLLKAYGELPE+TRLNEGI 
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEGIV 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           G +DD+DD  G+DY+EFEDEDID+I
Sbjct: 121 GDLDDDDDVVGEDYIEFEDEDIDRI 145

[Close]

BLAST of MS004933.1 vs. TAIR 10
Match: AT5G35680.2 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 266.9 bits (681), Expect = 9.3e-72
Identity = 130/145 (89.66%), Postives = 138/145 (95.17%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKREL+FKEDGQEYAQVLRMLGNGRC+ MCIDG KRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYM DEARLLKAYGELPE+TRLNEGI 
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEGIV 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           G +DD+DD  G+DY+EFEDEDID+I
Sbjct: 121 GDLDDDDDVVGEDYIEFEDEDIDRI 145

[Close]

BLAST of MS004933.1 vs. TAIR 10
Match: AT5G35680.3 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 243.8 bits (621), Expect = 8.4e-65
Identity = 118/128 (92.19%), Postives = 123/128 (96.09%), Query Frame = 0


Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKREL+FKEDGQEYAQVLRMLGNGRC+ MCIDG KRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYM DEARLLKAYGELPE+TRLNEGI 
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEGIV 120

Query: 121 GGIDDEDD 129
           G +DD+DD
Sbjct: 121 GDLDDDDD 128

The following BLAST results are available for this feature:

BLAST of MS004933.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140781.1	6.5e-75	100.00	eukaryotic translation initiation factor 1A-like [Momordica charantia]	[more]
XP_008457652.1	1.9e-74	99.31	PREDICTED: eukaryotic translation initiation factor 1A [Cucumis melo] >XP_008457...	[more]
XP_022145199.1	9.3e-74	98.62	eukaryotic translation initiation factor 1A [Momordica charantia] >XP_022956094....	[more]
XP_004147021.1	6.0e-73	97.24	eukaryotic translation initiation factor 1A [Cucumis sativus] >XP_011649283.1 eu...	[more]
CAA2961420.1	7.9e-73	96.55	eukaryotic translation initiation factor 1A [Olea europaea subsp. europaea]	[more]

BLAST of MS004933.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P56331	9.4e-69	91.10	Eukaryotic translation initiation factor 1A OS=Onobrychis viciifolia OX=3882 PE=...	[more]
P47815	2.4e-64	84.25	Eukaryotic translation initiation factor 1A OS=Triticum aestivum OX=4565 PE=1 SV...	[more]
P47813	3.0e-51	73.10	Eukaryotic translation initiation factor 1A, X-chromosomal OS=Homo sapiens OX=96...	[more]
Q8BMJ3	3.0e-51	73.10	Eukaryotic translation initiation factor 1A, X-chromosomal OS=Mus musculus OX=10...	[more]
Q5RA42	3.0e-51	73.10	Eukaryotic translation initiation factor 1A, X-chromosomal OS=Pongo abelii OX=96...	[more]

BLAST of MS004933.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CH30	3.1e-75	100.00	Eukaryotic translation initiation factor 4C OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A1S3C6N4	9.1e-75	99.31	Eukaryotic translation initiation factor 4C OS=Cucumis melo OX=3656 GN=LOC103497...	[more]
A0A5A7TS70	9.1e-75	99.31	Eukaryotic translation initiation factor 4C OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A6J1IS99	4.5e-74	98.62	Eukaryotic translation initiation factor 4C OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1GY26	4.5e-74	98.62	Eukaryotic translation initiation factor 4C OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of MS004933.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04520.1	3.2e-72	90.34	Nucleic acid-binding, OB-fold-like protein	[more]
AT5G35680.1	9.3e-72	89.66	Nucleic acid-binding, OB-fold-like protein	[more]
AT5G35680.2	9.3e-72	89.66	Nucleic acid-binding, OB-fold-like protein	[more]
AT5G35680.3	8.4e-65	92.19	Nucleic acid-binding, OB-fold-like protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001253	Translation initiation factor 1A (eIF-1A)	SMART	SM00652	eIF1neu4	coord: 28..110 e-value: 7.0E-49 score: 178.4
IPR001253	Translation initiation factor 1A (eIF-1A)	TIGRFAM	TIGR00523	TIGR00523	coord: 14..110 e-value: 8.1E-35 score: 116.8
IPR001253	Translation initiation factor 1A (eIF-1A)	PANTHER	PTHR21668	EIF-1A	coord: 1..145
IPR001253	Translation initiation factor 1A (eIF-1A)	HAMAP	MF_00216	aIF_1A	coord: 16..110 score: 18.345238
IPR001253	Translation initiation factor 1A (eIF-1A)	CDD	cd05793	S1_IF1A	coord: 33..109 e-value: 6.52876E-42 score: 132.262
None	No IPR available	GENE3D	2.40.50.140		coord: 2..145 e-value: 1.1E-57 score: 195.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR21668:SF18	EUKARYOTIC TRANSLATION INITIATION FACTOR 1A-LIKE	coord: 1..145
IPR006196	RNA-binding domain, S1, IF1 type	PFAM	PF01176	eIF-1a	coord: 32..93 e-value: 4.8E-22 score: 77.6
IPR006196	RNA-binding domain, S1, IF1 type	PROSITE	PS50832	S1_IF1_TYPE	coord: 22..96 score: 31.623802
IPR018104	Translation initiation factor 1A (eIF-1A), conserved site	PROSITE	PS01262	IF1A	coord: 41..63
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 2..144

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS004933	MS004933	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS004933.1-cds	MS004933.1-cds-scaffold176:1493926..1494360	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS004933.1	MS004933.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006413	translational initiation
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003743	translation initiation factor activity