MS004933.1 (mRNA) Bitter gourd (TR) v1

Overview
NameMS004933.1
TypemRNA
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionEukaryotic translation initiation factor 4C
Locationscaffold176: 1493926 .. 1494360 (+)
Sequence length435
RNA-Seq ExpressionMS004933.1
SyntenyMS004933.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAAAGAATAAGGGAAAGGGAGGAAAGAACAGGAAGAGAGGAAAGAATGAAGCAGATGATGAGAAGCGTGAACTTGTGTTCAAGGAAGATGGTCAGGAGTATGCACAAGTTCTCAGAATGTTGGGAAATGGTCGTTGTGAGGCAATGTGCATAGATGGGACAAAGCGCCTTTGCCATATTCGGGGGAAGATGCACAAGAAGGTTTGGATAGCGGCTGGTGATATTATACTTGTTGGTCTTCGCGACTATCAGGATGACAAAGCTGATGTTATCCTCAAGTATATGCCTGATGAAGCAAGGCTGTTGAAGGCATATGGTGAGCTTCCTGAGAGCACACGTCTGAACGAGGGTATTGCTGGTGGTATCGACGATGAGGATGATGCTGGGGGTGATGATTATCTTGAGTTTGAGGATGAAGATATTGATAAGATT

mRNA sequence

ATGCCAAAGAATAAGGGAAAGGGAGGAAAGAACAGGAAGAGAGGAAAGAATGAAGCAGATGATGAGAAGCGTGAACTTGTGTTCAAGGAAGATGGTCAGGAGTATGCACAAGTTCTCAGAATGTTGGGAAATGGTCGTTGTGAGGCAATGTGCATAGATGGGACAAAGCGCCTTTGCCATATTCGGGGGAAGATGCACAAGAAGGTTTGGATAGCGGCTGGTGATATTATACTTGTTGGTCTTCGCGACTATCAGGATGACAAAGCTGATGTTATCCTCAAGTATATGCCTGATGAAGCAAGGCTGTTGAAGGCATATGGTGAGCTTCCTGAGAGCACACGTCTGAACGAGGGTATTGCTGGTGGTATCGACGATGAGGATGATGCTGGGGGTGATGATTATCTTGAGTTTGAGGATGAAGATATTGATAAGATT

Coding sequence (CDS)

ATGCCAAAGAATAAGGGAAAGGGAGGAAAGAACAGGAAGAGAGGAAAGAATGAAGCAGATGATGAGAAGCGTGAACTTGTGTTCAAGGAAGATGGTCAGGAGTATGCACAAGTTCTCAGAATGTTGGGAAATGGTCGTTGTGAGGCAATGTGCATAGATGGGACAAAGCGCCTTTGCCATATTCGGGGGAAGATGCACAAGAAGGTTTGGATAGCGGCTGGTGATATTATACTTGTTGGTCTTCGCGACTATCAGGATGACAAAGCTGATGTTATCCTCAAGTATATGCCTGATGAAGCAAGGCTGTTGAAGGCATATGGTGAGCTTCCTGAGAGCACACGTCTGAACGAGGGTATTGCTGGTGGTATCGACGATGAGGATGATGCTGGGGGTGATGATTATCTTGAGTTTGAGGATGAAGATATTGATAAGATT

Protein sequence

MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIAGGIDDEDDAGGDDYLEFEDEDIDKI
Homology
BLAST of MS004933.1 vs. NCBI nr
Match: XP_022140781.1 (eukaryotic translation initiation factor 1A-like [Momordica charantia])

HSP 1 Score: 290.8 bits (743), Expect = 6.5e-75
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDDAGGDDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 145

BLAST of MS004933.1 vs. NCBI nr
Match: XP_008457652.1 (PREDICTED: eukaryotic translation initiation factor 1A [Cucumis melo] >XP_008457653.1 PREDICTED: eukaryotic translation initiation factor 1A [Cucumis melo] >KAA0045698.1 eukaryotic translation initiation factor 1A [Cucumis melo var. makuwa])

HSP 1 Score: 289.3 bits (739), Expect = 1.9e-74
Identity = 144/145 (99.31%), Postives = 144/145 (99.31%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD GGDDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDGGGDDYLEFEDEDIDKI 145

BLAST of MS004933.1 vs. NCBI nr
Match: XP_022145199.1 (eukaryotic translation initiation factor 1A [Momordica charantia] >XP_022956094.1 eukaryotic translation initiation factor 1A [Cucurbita moschata] >XP_022956095.1 eukaryotic translation initiation factor 1A [Cucurbita moschata] >XP_022979975.1 eukaryotic translation initiation factor 1A [Cucurbita maxima] >XP_022979976.1 eukaryotic translation initiation factor 1A [Cucurbita maxima] >XP_023527417.1 eukaryotic translation initiation factor 1A [Cucurbita pepo subsp. pepo] >XP_023527418.1 eukaryotic translation initiation factor 1A [Cucurbita pepo subsp. pepo] >XP_038902096.1 eukaryotic translation initiation factor 1A [Benincasa hispida] >XP_038902102.1 eukaryotic translation initiation factor 1A [Benincasa hispida])

HSP 1 Score: 287.0 bits (733), Expect = 9.3e-74
Identity = 143/145 (98.62%), Postives = 143/145 (98.62%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD G DDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDGGADDYLEFEDEDIDKI 145

BLAST of MS004933.1 vs. NCBI nr
Match: XP_004147021.1 (eukaryotic translation initiation factor 1A [Cucumis sativus] >XP_011649283.1 eukaryotic translation initiation factor 1A [Cucumis sativus] >KGN61900.1 hypothetical protein Csa_006324 [Cucumis sativus])

HSP 1 Score: 284.3 bits (726), Expect = 6.0e-73
Identity = 141/145 (97.24%), Postives = 143/145 (98.62%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD  GD+Y+EFEDEDIDKI
Sbjct: 121 GGIDDEDDGAGDNYIEFEDEDIDKI 145

BLAST of MS004933.1 vs. NCBI nr
Match: CAA2961420.1 (eukaryotic translation initiation factor 1A [Olea europaea subsp. europaea])

HSP 1 Score: 283.9 bits (725), Expect = 7.9e-73
Identity = 140/145 (96.55%), Postives = 143/145 (98.62%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEA CIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEATCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GG+D+EDD GGDDY+EFEDEDIDKI
Sbjct: 121 GGLDEEDDGGGDDYIEFEDEDIDKI 145

BLAST of MS004933.1 vs. ExPASy Swiss-Prot
Match: P56331 (Eukaryotic translation initiation factor 1A OS=Onobrychis viciifolia OX=3882 PE=2 SV=2)

HSP 1 Score: 260.8 bits (665), Expect = 9.4e-69
Identity = 133/146 (91.10%), Postives = 140/146 (95.89%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADD+KRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDDKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGI- 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILK MPDEARLLKAYGELP++TRLNEGI 
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKLMPDEARLLKAYGELPDNTRLNEGIG 120

Query: 121 AGGIDDEDDAGGDDYLEFEDEDIDKI 146
           AGG+D+E D   +DY+EFEDEDIDKI
Sbjct: 121 AGGLDEEMDT-ANDYIEFEDEDIDKI 145

BLAST of MS004933.1 vs. ExPASy Swiss-Prot
Match: P47815 (Eukaryotic translation initiation factor 1A OS=Triticum aestivum OX=4565 PE=1 SV=2)

HSP 1 Score: 246.1 bits (627), Expect = 2.4e-64
Identity = 123/146 (84.25%), Postives = 135/146 (92.47%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADD+KRELVFKEDGQEYAQV RMLGNGRCEA+C+DGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDDKRELVFKEDGQEYAQVTRMLGNGRCEAICVDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDI+LVGLRDYQDDKADVILKYM DEARLLKAYGELP++ RLNEG+ 
Sbjct: 61  IRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYMNDEARLLKAYGELPDTVRLNEGV- 120

Query: 121 GGIDDEDDAGGD-DYLEFEDEDIDKI 146
             +D  ++  GD DY++FEDEDIDKI
Sbjct: 121 -DVDGPEEGEGDSDYIQFEDEDIDKI 144

BLAST of MS004933.1 vs. ExPASy Swiss-Prot
Match: P47813 (Eukaryotic translation initiation factor 1A, X-chromosomal OS=Homo sapiens OX=9606 GN=EIF1AX PE=1 SV=2)

HSP 1 Score: 202.6 bits (514), Expect = 3.0e-51
Identity = 106/145 (73.10%), Postives = 118/145 (81.38%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNR+RGKNE + EKRELVFKEDGQEYAQV++MLGNGR EAMC DG KRLCH
Sbjct: 1   MPKNKGKGGKNRRRGKNENESEKRELVFKEDGQEYAQVIKMLGNGRLEAMCFDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGK+ KKVWI   DIILVGLRDYQD+KADVILKY  DEAR LKAYGELPE  ++NE   
Sbjct: 61  IRGKLRKKVWINTSDIILVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDT 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
            G  D+D+   DD +  +DEDID I
Sbjct: 121 FGPGDDDEIQFDD-IGDDDEDIDDI 144

BLAST of MS004933.1 vs. ExPASy Swiss-Prot
Match: Q8BMJ3 (Eukaryotic translation initiation factor 1A, X-chromosomal OS=Mus musculus OX=10090 GN=Eif1ax PE=2 SV=3)

HSP 1 Score: 202.6 bits (514), Expect = 3.0e-51
Identity = 106/145 (73.10%), Postives = 118/145 (81.38%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNR+RGKNE + EKRELVFKEDGQEYAQV++MLGNGR EAMC DG KRLCH
Sbjct: 1   MPKNKGKGGKNRRRGKNENESEKRELVFKEDGQEYAQVIKMLGNGRLEAMCFDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGK+ KKVWI   DIILVGLRDYQD+KADVILKY  DEAR LKAYGELPE  ++NE   
Sbjct: 61  IRGKLRKKVWINTSDIILVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDT 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
            G  D+D+   DD +  +DEDID I
Sbjct: 121 FGPGDDDEIQFDD-IGDDDEDIDDI 144

BLAST of MS004933.1 vs. ExPASy Swiss-Prot
Match: Q5RA42 (Eukaryotic translation initiation factor 1A, X-chromosomal OS=Pongo abelii OX=9601 GN=EIF1AX PE=2 SV=3)

HSP 1 Score: 202.6 bits (514), Expect = 3.0e-51
Identity = 106/145 (73.10%), Postives = 118/145 (81.38%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNR+RGKNE + EKRELVFKEDGQEYAQV++MLGNGR EAMC DG KRLCH
Sbjct: 1   MPKNKGKGGKNRRRGKNENESEKRELVFKEDGQEYAQVIKMLGNGRLEAMCFDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGK+ KKVWI   DIILVGLRDYQD+KADVILKY  DEAR LKAYGELPE  ++NE   
Sbjct: 61  IRGKLRKKVWINTSDIILVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDT 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
            G  D+D+   DD +  +DEDID I
Sbjct: 121 FGPGDDDEIQFDD-IGDDDEDIDDI 144

BLAST of MS004933.1 vs. ExPASy TrEMBL
Match: A0A6J1CH30 (Eukaryotic translation initiation factor 4C OS=Momordica charantia OX=3673 GN=LOC111011361 PE=3 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 3.1e-75
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDDAGGDDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 145

BLAST of MS004933.1 vs. ExPASy TrEMBL
Match: A0A1S3C6N4 (Eukaryotic translation initiation factor 4C OS=Cucumis melo OX=3656 GN=LOC103497302 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 9.1e-75
Identity = 144/145 (99.31%), Postives = 144/145 (99.31%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD GGDDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDGGGDDYLEFEDEDIDKI 145

BLAST of MS004933.1 vs. ExPASy TrEMBL
Match: A0A5A7TS70 (Eukaryotic translation initiation factor 4C OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G002160 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 9.1e-75
Identity = 144/145 (99.31%), Postives = 144/145 (99.31%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD GGDDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDGGGDDYLEFEDEDIDKI 145

BLAST of MS004933.1 vs. ExPASy TrEMBL
Match: A0A6J1IS99 (Eukaryotic translation initiation factor 4C OS=Cucurbita maxima OX=3661 GN=LOC111479507 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.5e-74
Identity = 143/145 (98.62%), Postives = 143/145 (98.62%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD G DDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDGGADDYLEFEDEDIDKI 145

BLAST of MS004933.1 vs. ExPASy TrEMBL
Match: A0A6J1GY26 (Eukaryotic translation initiation factor 4C OS=Cucurbita moschata OX=3662 GN=LOC111457892 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.5e-74
Identity = 143/145 (98.62%), Postives = 143/145 (98.62%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           GGIDDEDD G DDYLEFEDEDIDKI
Sbjct: 121 GGIDDEDDGGADDYLEFEDEDIDKI 145

BLAST of MS004933.1 vs. TAIR 10
Match: AT2G04520.1 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 268.5 bits (685), Expect = 3.2e-72
Identity = 131/145 (90.34%), Postives = 139/145 (95.86%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKREL+FKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELIFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDI+LVGLRDYQDDKADVILKYM DEARLLKAYGELPE+TRLNEGI 
Sbjct: 61  IRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEGIV 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           G ++D+DD   DDY+EFEDEDID+I
Sbjct: 121 GDLEDDDDNNDDDYVEFEDEDIDRI 145

BLAST of MS004933.1 vs. TAIR 10
Match: AT5G35680.1 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 266.9 bits (681), Expect = 9.3e-72
Identity = 130/145 (89.66%), Postives = 138/145 (95.17%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKREL+FKEDGQEYAQVLRMLGNGRC+ MCIDG KRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYM DEARLLKAYGELPE+TRLNEGI 
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEGIV 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           G +DD+DD  G+DY+EFEDEDID+I
Sbjct: 121 GDLDDDDDVVGEDYIEFEDEDIDRI 145

BLAST of MS004933.1 vs. TAIR 10
Match: AT5G35680.2 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 266.9 bits (681), Expect = 9.3e-72
Identity = 130/145 (89.66%), Postives = 138/145 (95.17%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKREL+FKEDGQEYAQVLRMLGNGRC+ MCIDG KRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYM DEARLLKAYGELPE+TRLNEGI 
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEGIV 120

Query: 121 GGIDDEDDAGGDDYLEFEDEDIDKI 146
           G +DD+DD  G+DY+EFEDEDID+I
Sbjct: 121 GDLDDDDDVVGEDYIEFEDEDIDRI 145

BLAST of MS004933.1 vs. TAIR 10
Match: AT5G35680.3 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 243.8 bits (621), Expect = 8.4e-65
Identity = 118/128 (92.19%), Postives = 123/128 (96.09%), Query Frame = 0

Query: 1   MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCH 60
           MPKNKGKGGKNRKRGKNEADDEKREL+FKEDGQEYAQVLRMLGNGRC+ MCIDG KRLCH
Sbjct: 1   MPKNKGKGGKNRKRGKNEADDEKRELIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCH 60

Query: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYGELPESTRLNEGIA 120
           IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYM DEARLLKAYGELPE+TRLNEGI 
Sbjct: 61  IRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEGIV 120

Query: 121 GGIDDEDD 129
           G +DD+DD
Sbjct: 121 GDLDDDDD 128

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140781.16.5e-75100.00eukaryotic translation initiation factor 1A-like [Momordica charantia][more]
XP_008457652.11.9e-7499.31PREDICTED: eukaryotic translation initiation factor 1A [Cucumis melo] >XP_008457... [more]
XP_022145199.19.3e-7498.62eukaryotic translation initiation factor 1A [Momordica charantia] >XP_022956094.... [more]
XP_004147021.16.0e-7397.24eukaryotic translation initiation factor 1A [Cucumis sativus] >XP_011649283.1 eu... [more]
CAA2961420.17.9e-7396.55eukaryotic translation initiation factor 1A [Olea europaea subsp. europaea][more]
Match NameE-valueIdentityDescription
P563319.4e-6991.10Eukaryotic translation initiation factor 1A OS=Onobrychis viciifolia OX=3882 PE=... [more]
P478152.4e-6484.25Eukaryotic translation initiation factor 1A OS=Triticum aestivum OX=4565 PE=1 SV... [more]
P478133.0e-5173.10Eukaryotic translation initiation factor 1A, X-chromosomal OS=Homo sapiens OX=96... [more]
Q8BMJ33.0e-5173.10Eukaryotic translation initiation factor 1A, X-chromosomal OS=Mus musculus OX=10... [more]
Q5RA423.0e-5173.10Eukaryotic translation initiation factor 1A, X-chromosomal OS=Pongo abelii OX=96... [more]
Match NameE-valueIdentityDescription
A0A6J1CH303.1e-75100.00Eukaryotic translation initiation factor 4C OS=Momordica charantia OX=3673 GN=LO... [more]
A0A1S3C6N49.1e-7599.31Eukaryotic translation initiation factor 4C OS=Cucumis melo OX=3656 GN=LOC103497... [more]
A0A5A7TS709.1e-7599.31Eukaryotic translation initiation factor 4C OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1IS994.5e-7498.62Eukaryotic translation initiation factor 4C OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1GY264.5e-7498.62Eukaryotic translation initiation factor 4C OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT2G04520.13.2e-7290.34Nucleic acid-binding, OB-fold-like protein [more]
AT5G35680.19.3e-7289.66Nucleic acid-binding, OB-fold-like protein [more]
AT5G35680.29.3e-7289.66Nucleic acid-binding, OB-fold-like protein [more]
AT5G35680.38.4e-6592.19Nucleic acid-binding, OB-fold-like protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001253Translation initiation factor 1A (eIF-1A)SMARTSM00652eIF1neu4coord: 28..110
e-value: 7.0E-49
score: 178.4
IPR001253Translation initiation factor 1A (eIF-1A)TIGRFAMTIGR00523TIGR00523coord: 14..110
e-value: 8.1E-35
score: 116.8
IPR001253Translation initiation factor 1A (eIF-1A)PANTHERPTHR21668EIF-1Acoord: 1..145
IPR001253Translation initiation factor 1A (eIF-1A)HAMAPMF_00216aIF_1Acoord: 16..110
score: 18.345238
IPR001253Translation initiation factor 1A (eIF-1A)CDDcd05793S1_IF1Acoord: 33..109
e-value: 6.52876E-42
score: 132.262
NoneNo IPR availableGENE3D2.40.50.140coord: 2..145
e-value: 1.1E-57
score: 195.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR21668:SF18EUKARYOTIC TRANSLATION INITIATION FACTOR 1A-LIKEcoord: 1..145
IPR006196RNA-binding domain, S1, IF1 typePFAMPF01176eIF-1acoord: 32..93
e-value: 4.8E-22
score: 77.6
IPR006196RNA-binding domain, S1, IF1 typePROSITEPS50832S1_IF1_TYPEcoord: 22..96
score: 31.623802
IPR018104Translation initiation factor 1A (eIF-1A), conserved sitePROSITEPS01262IF1Acoord: 41..63
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 2..144

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MS004933MS004933gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MS004933.1-cdsMS004933.1-cds-scaffold176:1493926..1494360CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MS004933.1MS004933.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006413 translational initiation
molecular_function GO:0003723 RNA binding
molecular_function GO:0003743 translation initiation factor activity