MS001222.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS001222.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Protein PEROXIN-4
Location	scaffold36: 2416830 .. 2419836 (-)
Sequence length	465
RNA-Seq Expression	MS001222.1
Synteny	MS001222.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCTCAAGAGCAAGGTTGTTCAAGGAGTACAAAGAGGTTCAAAGAGAAAAGGTAGCAGATCCAGATATTCAATTGGTTTGTGACGATTCAAATATTTTTAAATGGACTGCCCTAATCAAGGTATGCTCTTGATGATCCAGATGAAAATTTTGATTCAAGAGTTGTTTCTTACCATAATTGATATGTATAATATTTAGGGACCATCAGAGACTCCTTTTGAAGGTGGCGTCTTCCAGCTTGCCTTTTCTGTACCTGAGCAATATCCATTGCAACCTCCTCAAGTGCGGTTCTTGACAAAAATCTTCCATCCAAATGTTCATTTTAAGGTAGAAGTCGCCGTGACCCTTTTCCTAGATCATACGCTGATTATGAGAACTTCAGTCACAATTGTTAATATTATTATTTTTCATGAACTAGTTGGCTAATAGCTGCATTATTTCCCATAATATCTTAACATGAACTGATGAAGTTACAACCAGGTGATATCTAGTAGATTTTGATATCCCCTGGGATCCCAAATGAGGGAACCTTGAGAGAACCTGCCAAATCCAACAAGATCTGACCTACTTTCCTGCGACAAGATTTTGATGACGATTATAAGGAGGCAGTCTTGGGATGGCTCAAGTAGTCAATGAGGGCTAATGTAAATAGCAAAGGATTTAGAGAGAATGAGTTCAAGCCATGGTGGCCACCTACCTACGATTTAATATCCTACGAGTTTCCTTGTCAACCAAATGTAGTAGGGTCAGGCGGTTGGTCCGTGAATAGTCGAGAAGTGCGCAAGTTGGCCCGGACACACGGATATAAAAAAAATGAGCCAGTCATTGGAGGAAGGGGACACACTTGTTTAAAGGAGAGGAGTTAACAGCATTAGGACCTTTCTCAATTAGTTAACAAATTATGTTAAGCATCCAATATCCATGCATGATGGTGAGTAGGGAGAGAATATATAGAAGGTGTTATATAGAGGCAGGAGAGAGGAATATTGGAAAAGAAATTCTGTGGTGTATCATATGTGGAGAGTCTTGGGACTCTCAAATCATCTTGTGAATTATAGAAATTGAGTAGATGATGTCCATATCATTGAAGGTACTGGGGAGTGCACTCAAAATTTGAAGAGCCTCATGGACGAGGTAGAGTTGAAATCGGGTTGGAGGAGTTTGACAATGACCTCTCCATCAAAGGTTATATGGGATTGCGAGTGGGAAGTTAGAGGTCTATTTGGTAACATTCTCGTTTTTTGTTTTCAATTTTTCAGTTCTTGAGAAACATAAGCGTTTGGTAACGGTTTTTGTTTCTCTTTTCTAATCCTCAAGAAACATTTCCAAAATTATATAGAAATTTTAGAAACTACAAAATAAAGTTTCTTTATCTCATTCCCATTTCTAGTTTTTTCCAAAACAAATAAAAGTATTATTTTATCACTGGCTTATAAAATACATAATAAATATTAAAAATAAATAAAACAAAAAAACTTTCAATATTTAAAAAAACTAATTTTCATATAAAAAATTAAATTTTTTTTTTAAATTTCAAATTTTAGAGAACGAGAAATAAGATATGGTTACCAAATATGTTTCTCTTTCTTTTTTTCAATAAAAAGTAAAGAATAAGAAATAAAAAACAAACGCAGTTCTATTTTTATTTATTAAAAAAGAGGAAATAAGAAACAAAAAATAAGAACATTACCAAATGGGCTCCAAGGGCTTTGATTTTTTTATGAAGAGGATACAAATAGATAGACGTAATTAGTGGGTTGCTTCTTCCAGATGAGTGTAATTCTAGTTGCAGAACAACTCGTTGCATAAGTGAGCAGCAGCCTTGAGGGAGAGATTTCGGTAAACCACTGGGAGACTTGGAGGAGGGTGATTTAGCCATGGTATGAGGTGAGAGCCCTAATACTTAGCATATTCCAGCCTTTGCAGGGGGCCTCACCAAGTGAAATTCATGGCACTCCGGCAAGTGGAAAGTATTATGAGATTGCCATTGGCAATTTGAAGGGCTTGCAGCGCCTTTGGGTGAAGTAATACAATTTGTATTCTTTGCTAATAAGAACTATATTGGTGAATGGTGACCATTCACGTTAGCTGATTCCTTTTGTGATTGTAGTCTTCTTTTAGAGTGAACCTTATTTTTAACGCACTTTATTGCTGTGATTAAATATATGAACATATATCTTTTTGGTTGTTGAACCACGCTTTACACTTATCACTGCATCTTCCTGCAGACGGGTGAGATCTGCCTCGATATTTTGAAGAATGCATGGAGCCCAGCGTGGACTCTGCAATCTGTTTGTAGGGCTATAATTGCATTGATGGCTCATCCAGAACCCGATAGCCCGCTGAACTGTGATTCTGGTGAGTGTAACATGGATTCTTTACAATGAATTTTCAGGATTTACATGTTTATTTATTTGGAATGGCGCGTGACAATTTGATAATGAAGCGAAGTAGTTAAATGATCTTAAAACTGATTCTTGCTGGCCACTGCGTTTCGCCACTCCAAAATTGATCTTTTGGAGAAGAATTTGCATGTAGTTAAAATGTGACGAAGGTATGCGATAGTTTGCACGTTTTTTTCAAAAAATGATGTCAGGAAACCCTACACTTTGCATTATGCCTGTGACTTATCTCAGCTATTCTTTGGGGAAGCTTTTCCCCTATCCCAAACCAACTTCTGGGGGCAACTAGAATTTTAAATTAGTAAGTTTTTCATCTAGAGCCTTGAACTTAGGACCTTGGAGATAAATCTTTAAACGTTCCGAATGGAGGTGGTCACTCCTGGAAGGCAAACTGACTGATGCAAGTTGCTCGATTTCGTCAACTTTTTTTACGAACATATAAAGCAGCTATTATTCCAGCAGTAAGTAGGAAGAGGGTAGAAATAAAAATCATTTTAACTGATTGTGTGGGCTTTCACAGGCAATCTTCTTCGGTCGGGTGATATCCGAGGATACCAATCCATGGCGAGGATGTACACAAGGCTTGCTGCCATGCCCAAAAAGGGC

mRNA sequence

GCCTCAAGAGCAAGGTTGTTCAAGGAGTACAAAGAGGTTCAAAGAGAAAAGGTAGCAGATCCAGATATTCAATTGGTTTGTGACGATTCAAATATTTTTAAATGGACTGCCCTAATCAAGGGACCATCAGAGACTCCTTTTGAAGGTGGCGTCTTCCAGCTTGCCTTTTCTGTACCTGAGCAATATCCATTGCAACCTCCTCAAGTGCGGTTCTTGACAAAAATCTTCCATCCAAATGTTCATTTTAAGACGGGTGAGATCTGCCTCGATATTTTGAAGAATGCATGGAGCCCAGCGTGGACTCTGCAATCTGTTTGTAGGGCTATAATTGCATTGATGGCTCATCCAGAACCCGATAGCCCGCTGAACTGTGATTCTGGCAATCTTCTTCGGTCGGGTGATATCCGAGGATACCAATCCATGGCGAGGATGTACACAAGGCTTGCTGCCATGCCCAAAAAGGGC

Coding sequence (CDS)

GCCTCAAGAGCAAGGTTGTTCAAGGAGTACAAAGAGGTTCAAAGAGAAAAGGTAGCAGATCCAGATATTCAATTGGTTTGTGACGATTCAAATATTTTTAAATGGACTGCCCTAATCAAGGGACCATCAGAGACTCCTTTTGAAGGTGGCGTCTTCCAGCTTGCCTTTTCTGTACCTGAGCAATATCCATTGCAACCTCCTCAAGTGCGGTTCTTGACAAAAATCTTCCATCCAAATGTTCATTTTAAGACGGGTGAGATCTGCCTCGATATTTTGAAGAATGCATGGAGCCCAGCGTGGACTCTGCAATCTGTTTGTAGGGCTATAATTGCATTGATGGCTCATCCAGAACCCGATAGCCCGCTGAACTGTGATTCTGGCAATCTTCTTCGGTCGGGTGATATCCGAGGATACCAATCCATGGCGAGGATGTACACAAGGCTTGCTGCCATGCCCAAAAAGGGC

Protein sequence

ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG

Homology

[Close]

BLAST of MS001222.1 vs. NCBI nr
Match: XP_022131340.1 (protein PEROXIN-4 [Momordica charantia] >CAA2934805.1 PEROXIN-4 [Olea europaea subsp. europaea])

HSP 1 Score: 325.9 bits (834), Expect = 1.9e-85
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE
Sbjct: 3   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
           QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 63  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG
Sbjct: 123 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of MS001222.1 vs. NCBI nr
Match: GFQ01985.1 (protein peroxin-4, partial [Phtheirospermum japonicum])

HSP 1 Score: 325.9 bits (834), Expect = 1.9e-85
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE
Sbjct: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
           QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG
Sbjct: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 155

[Close]

BLAST of MS001222.1 vs. NCBI nr
Match: XP_022950978.1 (protein PEROXIN-4 [Cucurbita moschata] >XP_022950979.1 protein PEROXIN-4 [Cucurbita moschata] >XP_022950980.1 protein PEROXIN-4 [Cucurbita moschata] >XP_023002537.1 protein PEROXIN-4 [Cucurbita maxima] >XP_023002538.1 protein PEROXIN-4 [Cucurbita maxima] >XP_023536751.1 protein PEROXIN-4 [Cucurbita pepo subsp. pepo] >KAG6585396.1 Protein PEROXIN-4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020312.1 Protein PEROXIN-4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 325.5 bits (833), Expect = 2.5e-85
Identity = 154/155 (99.35%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE
Sbjct: 3   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
           QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 63  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLRSGD+RGYQSMARMYTRLAAMPKKG
Sbjct: 123 PLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of MS001222.1 vs. NCBI nr
Match: XP_019197100.1 (PREDICTED: protein PEROXIN-4 [Ipomoea nil] >XP_031117903.1 protein PEROXIN-4 [Ipomoea triloba] >PSR91791.1 Protein PEROXIN-4 [Actinidia chinensis var. chinensis])

HSP 1 Score: 324.3 bits (830), Expect = 5.6e-85
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREK ADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE
Sbjct: 3   ASRARLFKEYKEVQREKAADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
           QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 63  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG
Sbjct: 123 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of MS001222.1 vs. NCBI nr
Match: KAG8375569.1 (hypothetical protein BUALT_Bualt10G0114200 [Buddleja alternifolia])

HSP 1 Score: 324.3 bits (830), Expect = 5.6e-85
Identity = 153/155 (98.71%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE
Sbjct: 3   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
           QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 63  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLR+GD+RGYQSMARMYTRLAAMPKKG
Sbjct: 123 PLNCDSGNLLRAGDVRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of MS001222.1 vs. ExPASy Swiss-Prot
Match: Q8LGF7 (Protein PEROXIN-4 OS=Arabidopsis thaliana OX=3702 GN=PEX4 PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 3.4e-85
Identity = 147/155 (94.84%), Postives = 153/155 (98.71%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREKVADPDIQL+CDD+NIFKWTALIKGPSETP+EGGVFQLAFSVPE
Sbjct: 3   ASRARLFKEYKEVQREKVADPDIQLICDDTNIFKWTALIKGPSETPYEGGVFQLAFSVPE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
            YPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 63  PYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLRSGD+RG+ SMA+MYTRLAAMPKKG
Sbjct: 123 PLNCDSGNLLRSGDVRGFNSMAQMYTRLAAMPKKG 157

[Close]

BLAST of MS001222.1 vs. ExPASy Swiss-Prot
Match: Q86IZ3 (Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostelium discoideum OX=44689 GN=pex4 PE=3 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 1.5e-51
Identity = 90/145 (62.07%), Postives = 119/145 (82.07%), Query Frame = 0


Query: 4   ARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPEQYP 63
           ARL KEYK +Q E+  D  +    D+S++++W A+IKGP +TP+E G F+L  SVP  YP
Sbjct: 3   ARLMKEYKVLQNEEFED-ILLYPRDESDLYRWVAIIKGPPDTPYENGKFELDISVPTNYP 62

Query: 64  LQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLN 123
           LQPP ++F+TKIFHPN+HFKTGEICLD+LK +WS  +TLQSVCR+IIAL++ PE DSPLN
Sbjct: 63  LQPPTIKFVTKIFHPNIHFKTGEICLDLLKTSWSAIYTLQSVCRSIIALLSLPEADSPLN 122

Query: 124 CDSGNLLRSGDIRGYQSMARMYTRL 149
           CD+GNL+R+GD++G+ S+ARMYTRL
Sbjct: 123 CDAGNLIRNGDVKGHDSLARMYTRL 146

[Close]

BLAST of MS001222.1 vs. ExPASy Swiss-Prot
Match: Q9P6I1 (Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ubc16 PE=3 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 7.3e-35
Identity = 72/153 (47.06%), Postives = 97/153 (63.40%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           +S  RL KEY   Q   ++   +     D ++F W A+I+GP+ETP+EGG + L   V E
Sbjct: 3   SSIKRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
            YP+ PP V F TKI HPN+ +  GE+C+DILK  WSPAW+LQS C AII+L+++ +  S
Sbjct: 63  GYPISPPSVYFQTKIVHPNISWTNGEVCMDILKTHWSPAWSLQSACLAIISLLSNYDASS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPK 154
           PLN D+  LLR+GD   Y S+ R  T L A  K
Sbjct: 123 PLNVDAAKLLRTGDKTAYNSLVRCTTYLYAKGK 155

[Close]

BLAST of MS001222.1 vs. ExPASy Swiss-Prot
Match: O60015 (Ubiquitin-conjugating enzyme E2-21 kDa OS=Pichia angusta OX=870730 GN=PEX4 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 5.7e-32
Identity = 68/174 (39.08%), Postives = 99/174 (56.90%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPD-------IQLVCDDSNIFKWTALIKGPSETPFEGGVFQ 60
           ++  RL KEY+ V++E             + L   +  +F+W+A+I+GP ++PFE  +++
Sbjct: 3   STEKRLLKEYRAVKKELTEKRSPIHDTGIVDLHPLEDGLFRWSAVIRGPDQSPFEDALWK 62

Query: 61  LAFSVPEQYPLQPPQVRFLT----------------------KIFHPNVHFKTGEICLDI 120
           L   +P  YPL PP+++F+                       K+ HPNV+FKTGEICLDI
Sbjct: 63  LEIDIPTNYPLDPPKIKFVVFGEEKIRQLQRKTSSGARKVCYKMPHPNVNFKTGEICLDI 122

Query: 121 LKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDIRGYQSMARMY 146
           L+  WSPAWTLQS   AI+ L+A+PEP SPLN D  NLL+  D   Y+ +   Y
Sbjct: 123 LQQKWSPAWTLQSALVAIVVLLANPEPLSPLNIDMANLLKCDDTTAYKDLVHYY 176

[Close]

BLAST of MS001222.1 vs. ExPASy Swiss-Prot
Match: Q9EQX9 (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus OX=10116 GN=Ube2n PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.0e-28
Identity = 66/147 (44.90%), Postives = 95/147 (64.63%), Query Frame = 0


Query: 5   RLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPEQYPL 64
           R+ KE + +  E V  P I+   D+SN   +  +I GP ++PFEGG F+L   +PE+YP+
Sbjct: 7   RIIKETQRLLAEPV--PGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPM 66

Query: 65  QPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNC 124
             P+VRF+TKI+HPNV  K G ICLDILK+ WSPA  +++V  +I AL++ P PD PL  
Sbjct: 67  AAPKVRFMTKIYHPNVD-KLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLAN 126

Query: 125 DSGNLLRSGDIRGYQSMARMYTRLAAM 152
           D     +S + +  ++ AR +TRL AM
Sbjct: 127 DVAEQWKSNEAQAIET-ARAWTRLYAM 149

[Close]

BLAST of MS001222.1 vs. ExPASy TrEMBL
Match: A0A6J1BPF8 (protein PEROXIN-4 OS=Momordica charantia OX=3673 GN=LOC111004594 PE=3 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 9.4e-86
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE
Sbjct: 3   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
           QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 63  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG
Sbjct: 123 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of MS001222.1 vs. ExPASy TrEMBL
Match: A0A6J1KTV6 (protein PEROXIN-4 OS=Cucurbita maxima OX=3661 GN=LOC111496350 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.2e-85
Identity = 154/155 (99.35%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE
Sbjct: 3   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
           QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 63  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLRSGD+RGYQSMARMYTRLAAMPKKG
Sbjct: 123 PLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of MS001222.1 vs. ExPASy TrEMBL
Match: A0A6J1GHB1 (protein PEROXIN-4 OS=Cucurbita moschata OX=3662 GN=LOC111453964 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.2e-85
Identity = 154/155 (99.35%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE
Sbjct: 3   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
           QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 63  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLRSGD+RGYQSMARMYTRLAAMPKKG
Sbjct: 123 PLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of MS001222.1 vs. ExPASy TrEMBL
Match: A0A2R6PIT1 (Protein PEROXIN-4 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc29138 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.7e-85
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREK ADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE
Sbjct: 3   ASRARLFKEYKEVQREKAADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
           QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 63  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG
Sbjct: 123 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of MS001222.1 vs. ExPASy TrEMBL
Match: A0A1S4DVS9 (protein PEROXIN-4 OS=Cucumis melo OX=3656 GN=LOC103488194 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.7e-85
Identity = 153/155 (98.71%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAF+VPE
Sbjct: 3   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFAVPE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
           QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 63  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLRSGD+RGYQSMARMYTRLAAMPKKG
Sbjct: 123 PLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]

BLAST of MS001222.1 vs. TAIR 10
Match: AT5G25760.1 (peroxin4 )

HSP 1 Score: 315.5 bits (807), Expect = 2.4e-86
Identity = 147/155 (94.84%), Postives = 153/155 (98.71%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREKVADPDIQL+CDD+NIFKWTALIKGPSETP+EGGVFQLAFSVPE
Sbjct: 3   ASRARLFKEYKEVQREKVADPDIQLICDDTNIFKWTALIKGPSETPYEGGVFQLAFSVPE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
            YPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 63  PYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLRSGD+RG+ SMA+MYTRLAAMPKKG
Sbjct: 123 PLNCDSGNLLRSGDVRGFNSMAQMYTRLAAMPKKG 157

[Close]

BLAST of MS001222.1 vs. TAIR 10
Match: AT5G25760.2 (peroxin4 )

HSP 1 Score: 315.5 bits (807), Expect = 2.4e-86
Identity = 147/155 (94.84%), Postives = 153/155 (98.71%), Query Frame = 0


Query: 1   ASRARLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPE 60
           ASRARLFKEYKEVQREKVADPDIQL+CDD+NIFKWTALIKGPSETP+EGGVFQLAFSVPE
Sbjct: 3   ASRARLFKEYKEVQREKVADPDIQLICDDTNIFKWTALIKGPSETPYEGGVFQLAFSVPE 62

Query: 61  QYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 120
            YPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS
Sbjct: 63  PYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDS 122

Query: 121 PLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 156
           PLNCDSGNLLRSGD+RG+ SMA+MYTRLAAMPKKG
Sbjct: 123 PLNCDSGNLLRSGDVRGFNSMAQMYTRLAAMPKKG 157

[Close]

BLAST of MS001222.1 vs. TAIR 10
Match: AT5G53300.1 (ubiquitin-conjugating enzyme 10 )

HSP 1 Score: 124.0 bits (310), Expect = 1.0e-28
Identity = 62/147 (42.18%), Postives = 89/147 (60.54%), Query Frame = 0


Query: 5   RLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPEQYPL 64
           R+ KE K++Q++         V +D  +F W A I GPSE+P+ GGVF +    P  YP 
Sbjct: 5   RILKELKDLQKDPPTSCSAGPVAED--MFHWQATIMGPSESPYAGGVFLVTIHFPPDYPF 64

Query: 65  QPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNC 124
           +PP+V F TK+FHPN++   G ICLDILK  WSPA T+  V  +I +L+  P PD PL  
Sbjct: 65  KPPKVAFRTKVFHPNIN-SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 124

Query: 125 DSGNLLRSGDIRGYQSMARMYTRLAAM 152
           +  ++ ++ D   Y+S AR +T+  AM
Sbjct: 125 EIAHMYKT-DKNKYESTARSWTQKYAM 147

[Close]

BLAST of MS001222.1 vs. TAIR 10
Match: AT5G53300.2 (ubiquitin-conjugating enzyme 10 )

HSP 1 Score: 124.0 bits (310), Expect = 1.0e-28
Identity = 62/147 (42.18%), Postives = 89/147 (60.54%), Query Frame = 0


Query: 5   RLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPEQYPL 64
           R+ KE K++Q++         V +D  +F W A I GPSE+P+ GGVF +    P  YP 
Sbjct: 5   RILKELKDLQKDPPTSCSAGPVAED--MFHWQATIMGPSESPYAGGVFLVTIHFPPDYPF 64

Query: 65  QPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNC 124
           +PP+V F TK+FHPN++   G ICLDILK  WSPA T+  V  +I +L+  P PD PL  
Sbjct: 65  KPPKVAFRTKVFHPNIN-SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 124

Query: 125 DSGNLLRSGDIRGYQSMARMYTRLAAM 152
           +  ++ ++ D   Y+S AR +T+  AM
Sbjct: 125 EIAHMYKT-DKNKYESTARSWTQKYAM 147

[Close]

BLAST of MS001222.1 vs. TAIR 10
Match: AT5G53300.4 (ubiquitin-conjugating enzyme 10 )

HSP 1 Score: 124.0 bits (310), Expect = 1.0e-28
Identity = 62/147 (42.18%), Postives = 89/147 (60.54%), Query Frame = 0


Query: 5   RLFKEYKEVQREKVADPDIQLVCDDSNIFKWTALIKGPSETPFEGGVFQLAFSVPEQYPL 64
           R+ KE K++Q++         V +D  +F W A I GPSE+P+ GGVF +    P  YP 
Sbjct: 5   RILKELKDLQKDPPTSCSAGPVAED--MFHWQATIMGPSESPYAGGVFLVTIHFPPDYPF 64

Query: 65  QPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNC 124
           +PP+V F TK+FHPN++   G ICLDILK  WSPA T+  V  +I +L+  P PD PL  
Sbjct: 65  KPPKVAFRTKVFHPNIN-SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 124

Query: 125 DSGNLLRSGDIRGYQSMARMYTRLAAM 152
           +  ++ ++ D   Y+S AR +T+  AM
Sbjct: 125 EIAHMYKT-DKNKYESTARSWTQKYAM 147

The following BLAST results are available for this feature:

BLAST of MS001222.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131340.1	1.9e-85	100.00	protein PEROXIN-4 [Momordica charantia] >CAA2934805.1 PEROXIN-4 [Olea europaea s...	[more]
GFQ01985.1	1.9e-85	100.00	protein peroxin-4, partial [Phtheirospermum japonicum]	[more]
XP_022950978.1	2.5e-85	99.35	protein PEROXIN-4 [Cucurbita moschata] >XP_022950979.1 protein PEROXIN-4 [Cucurb...	[more]
XP_019197100.1	5.6e-85	99.35	PREDICTED: protein PEROXIN-4 [Ipomoea nil] >XP_031117903.1 protein PEROXIN-4 [Ip...	[more]
KAG8375569.1	5.6e-85	98.71	hypothetical protein BUALT_Bualt10G0114200 [Buddleja alternifolia]	[more]

BLAST of MS001222.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LGF7	3.4e-85	94.84	Protein PEROXIN-4 OS=Arabidopsis thaliana OX=3702 GN=PEX4 PE=1 SV=1	[more]
Q86IZ3	1.5e-51	62.07	Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostelium discoideum OX=44689 GN=pex...	[more]
Q9P6I1	7.3e-35	47.06	Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccharomyces pombe (strain 972 / AT...	[more]
O60015	5.7e-32	39.08	Ubiquitin-conjugating enzyme E2-21 kDa OS=Pichia angusta OX=870730 GN=PEX4 PE=1 ...	[more]
Q9EQX9	5.0e-28	44.90	Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus OX=10116 GN=Ube2n PE=1 SV...	[more]

BLAST of MS001222.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BPF8	9.4e-86	100.00	protein PEROXIN-4 OS=Momordica charantia OX=3673 GN=LOC111004594 PE=3 SV=1	[more]
A0A6J1KTV6	1.2e-85	99.35	protein PEROXIN-4 OS=Cucurbita maxima OX=3661 GN=LOC111496350 PE=3 SV=1	[more]
A0A6J1GHB1	1.2e-85	99.35	protein PEROXIN-4 OS=Cucurbita moschata OX=3662 GN=LOC111453964 PE=3 SV=1	[more]
A0A2R6PIT1	2.7e-85	99.35	Protein PEROXIN-4 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc2...	[more]
A0A1S4DVS9	2.7e-85	98.71	protein PEROXIN-4 OS=Cucumis melo OX=3656 GN=LOC103488194 PE=3 SV=1	[more]

BLAST of MS001222.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25760.1	2.4e-86	94.84	peroxin4	[more]
AT5G25760.2	2.4e-86	94.84	peroxin4	[more]
AT5G53300.1	1.0e-28	42.18	ubiquitin-conjugating enzyme 10	[more]
AT5G53300.2	1.0e-28	42.18	ubiquitin-conjugating enzyme 10	[more]
AT5G53300.4	1.0e-28	42.18	ubiquitin-conjugating enzyme 10	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 4..151 e-value: 1.1E-53 score: 194.4
None	No IPR available	PANTHER	PTHR24068	UBIQUITIN-CONJUGATING ENZYME E2	coord: 2..155
None	No IPR available	PANTHER	PTHR24068:SF383	BNAA09G04490D PROTEIN	coord: 2..155
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 5..145 e-value: 4.5E-46 score: 156.0
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 1..151 score: 52.012379
IPR000608	Ubiquitin-conjugating enzyme E2	CDD	cd00195	UBCc	coord: 5..146 e-value: 5.89079E-55 score: 167.764
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 1..150 e-value: 1.6E-52 score: 179.5
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 3..151
IPR023313	Ubiquitin-conjugating enzyme, active site	PROSITE	PS00183	UBC_1	coord: 76..92

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS001222	MS001222	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS001222.1-cds	MS001222.1-cds-scaffold36:2416830..2416915	CDS
MS001222.1-cds	MS001222.1-cds-scaffold36:2417480..2417609	CDS
MS001222.1-cds	MS001222.1-cds-scaffold36:2419511..2419639	CDS
MS001222.1-cds	MS001222.1-cds-scaffold36:2419717..2419836	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS001222.1	MS001222.1-protein	polypeptide