MELO3C022228.jh1.t2 (mRNA) Melon (Harukei-3) v1.41

Overview


Name	MELO3C022228.jh1.t2
Type	mRNA
Organism	Cucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Description	DLH domain-containing protein
Location	chr09: 58618 .. 60590 (-)
Sequence length	1525
RNA-Seq Expression	MELO3C022228.jh1.t2
Synteny	MELO3C022228.jh1.t2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSstart_codonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTACAATCATTTCTACCGTTTAAACTTTAAACTTAATACTAGCAAGTTGGATTGTGCAGAGCTTGTCACCGCACAATTAATTTTGTCTTGCGGGTTGAGTTGTTTTCCCCTCATTGATTGTCGATCCATTTGTCTAGTGTCAATGGAGTCTATCTAGATATTTTAGGAAGGACAAAACAAGAAGAAGAAGAAGATAAGTTTAAAAGAAAAATGAAGATAAGAAATTGGTGATCTACATGGAAACTTATAGGTTGCGTCTGTGGGATTTTATGTCCCCAACTCCCAACAGGATATTTTTAGGATATTTGTTTTGGACGTCATCCATTGGCTGCTCTGCAAATCTTTTGTTGGCTAAGAGAGAATGGGATTTGCCTCTGCCGGAGCGCAGTTGATTTCCCTTCCACCAACTGGGAGCAGTAGCCGCCGTGTCGAGAAGCCAATTCAGCCACCTTACTCCCAATTTGCTTATCCTCTCCCTTCTGTATGTTTATTTTCCCTTTTCTTCTCTACCTTAAATGTATTCCCCAACATGTTTACTACTATGATGGAATCCTCATTTTCTTAAGAGTAAAGGATTGAGCTTTCTTTTTTTTAGTGGAAATACAATAATTGGGGTGATTGGAAATGGAAGGGTAAACTGCGTTATTATAGCGTAAAGGGCCGAGTGAAGGATGATGATGACGACGCTTGTGAACTAGTGAATGGAATGGAGCTTTCAATTGGGGAACAACCTCATCATTCAATTCCTGCATATCTCTTTAAGGCAGTCAAGAACAATAATGGAACTGCTATTCTTCTCTTGTCCGATCTCTTTGCCTTTCAAGATTCTTCCATTAGAGACTTTGCTTATCGTCTCGCTTGCAATGGCTTCAAGTATGCTGCCCTAACATTCACATTCAATTCCAATTTTGTATCAAATAATATGTAAATGTTAAAATAATAAATCAAAGTTAACTAAATATATATTATAACAATAGAAAAATATTTATTGCTCTTTCATTTTGAATTATGTTTAAGAAATTTCAATTACAAAAAAAATATTAATAAACATTGTTTTGAAGAATTGTTAAAAAATATTTATAAAAAGTAGAAAATTTTCAAACCTATACATTTATAGAATCAATGATAGTAAATTAGATTGAAATTAAAAATACACTTTGAAACAAGGTTTAAAAGTTGTGTCCCGTAACATTTTGTGCCAAATGCATTTTTTTCCCTCTTAACGACGCCACAATTGTTTGCTGAAGTTGAACTGCAGTGTCTTACTTCCAGATTTAGTTAACGAAGATCCATGGGAGAAACCGCCAGCTCAACACTCTGCAAAACAAGAGATGCAGAGGATTGCAGAAGACATAGGAACAGTAACAAAATGGTTGACCGACGAGTTTTCAGCGGCAGGCCTCTCAAGGAAGCTTGGGATCATAGGATTTGGTTATGGAGGAGGCCGAGTAATAGATGTTCTAGCTCGAGACAAGGGTGCCTGTTTCTTGATTGGAGCCTCCCTCTATGGCACTGAGGTAGATCCATCACTTGCTACCAAAGTCAAGGTTCCTGTGTTGCTCATCTCAGGGGACGATGACCCACTTTGTCCAGTGAGTGTCATGAAGGATTTGGAGAAAGGGATGGGAGAAGGTTCCAAGTTAGTGATATTTGAAGGAAGAGGCCATGGCTTTGTTCACAATCCTGGATCTCCAGAAGAAGATGATGATGCTGAACAGGCTTTTCTAATGCTTAGAAATTGGCTACATCACGGCTTGATTGCAACTAATAATTTAGCTAACTGCCATGGGTAATATAGTAATAAATGAAATCAATCTGATTCTTGTCCCCGTTACTTCGATTCCAAAATGCCGAAGGCCAGGGGGATGCCGCACTTTTACGCAATTCAGCAATGCGATGAAGTCATTAATAAACACATACTTTCAATTGGTGGGATCAATGTGTACATAATTTCTAGGCTAAAATTGATTTT

mRNA sequence

CTTACAATCATTTCTACCGTTTAAACTTTAAACTTAATACTAGCAAGTTGGATTGTGCAGAGCTTGTCACCGCACAATTAATTTTGTCTTGCGGGTTGAGTTGTTTTCCCCTCATTGATTGTCGATCCATTTGTCTAGTGTCAATGGAGTCTATCTAGATATTTTAGGAAGGACAAAACAAGAAGAAGAAGAAGATAAGTTTAAAAGAAAAATGAAGATAAGAAATTGGTGATCTACATGGAAACTTATAGGTTGCGTCTGTGGGATTTTATGTCCCCAACTCCCAACAGGATATTTTTAGGATATTTGTTTTGGACGTCATCCATTGGCTGCTCTGCAAATCTTTTGTTGGCTAAGAGAGAATGGGATTTGCCTCTGCCGGAGCGCAGTTGATTTCCCTTCCACCAACTGGGAGCAGTAGCCGCCGTGTCGAGAAGCCAATTCAGCCACCTTACTCCCAATTTGCTTATCCTCTCCCTTCTTGGAAATACAATAATTGGGGTGATTGGAAATGGAAGGGTAAACTGCGTTATTATAGCGTAAAGGGCCGAGTGAAGGATGATGATGACGACGCTTGTGAACTAGTGAATGGAATGGAGCTTTCAATTGGGGAACAACCTCATCATTCAATTCCTGCATATCTCTTTAAGGCAGTCAAGAACAATAATGGAACTGCTATTCTTCTCTTGTCCGATCTCTTTGCCTTTCAAGATTCTTCCATTAGAGACTTTGCTTATCGTCTCGCTTGCAATGGCTTCAAGTATGCTGCCCTAACATTCACATTCAATTCCAATTTTGTATCAAATAATATTGTCTTACTTCCAGATTTAGTTAACGAAGATCCATGGGAGAAACCGCCAGCTCAACACTCTGCAAAACAAGAGATGCAGAGGATTGCAGAAGACATAGGAACAGTAACAAAATGGTTGACCGACGAGTTTTCAGCGGCAGGCCTCTCAAGGAAGCTTGGGATCATAGGATTTGGTTATGGAGGAGGCCGAGTAATAGATGTTCTAGCTCGAGACAAGGGTGCCTGTTTCTTGATTGGAGCCTCCCTCTATGGCACTGAGGTAGATCCATCACTTGCTACCAAAGTCAAGGTTCCTGTGTTGCTCATCTCAGGGGACGATGACCCACTTTGTCCAGTGAGTGTCATGAAGGATTTGGAGAAAGGGATGGGAGAAGGTTCCAAGTTAGTGATATTTGAAGGAAGAGGCCATGGCTTTGTTCACAATCCTGGATCTCCAGAAGAAGATGATGATGCTGAACAGGCTTTTCTAATGCTTAGAAATTGGCTACATCACGGCTTGATTGCAACTAATAATTTAGCTAACTGCCATGGGTAATATAGTAATAAATGAAATCAATCTGATTCTTGTCCCCGTTACTTCGATTCCAAAATGCCGAAGGCCAGGGGGATGCCGCACTTTTACGCAATTCAGCAATGCGATGAAGTCATTAATAAACACATACTTTCAATTGGTGGGATCAATGTGTACATAATTTCTAGGCTAAAATTGATTTT

Coding sequence (CDS)

ATGGGATTTGCCTCTGCCGGAGCGCAGTTGATTTCCCTTCCACCAACTGGGAGCAGTAGCCGCCGTGTCGAGAAGCCAATTCAGCCACCTTACTCCCAATTTGCTTATCCTCTCCCTTCTTGGAAATACAATAATTGGGGTGATTGGAAATGGAAGGGTAAACTGCGTTATTATAGCGTAAAGGGCCGAGTGAAGGATGATGATGACGACGCTTGTGAACTAGTGAATGGAATGGAGCTTTCAATTGGGGAACAACCTCATCATTCAATTCCTGCATATCTCTTTAAGGCAGTCAAGAACAATAATGGAACTGCTATTCTTCTCTTGTCCGATCTCTTTGCCTTTCAAGATTCTTCCATTAGAGACTTTGCTTATCGTCTCGCTTGCAATGGCTTCAAGTATGCTGCCCTAACATTCACATTCAATTCCAATTTTGTATCAAATAATATTGTCTTACTTCCAGATTTAGTTAACGAAGATCCATGGGAGAAACCGCCAGCTCAACACTCTGCAAAACAAGAGATGCAGAGGATTGCAGAAGACATAGGAACAGTAACAAAATGGTTGACCGACGAGTTTTCAGCGGCAGGCCTCTCAAGGAAGCTTGGGATCATAGGATTTGGTTATGGAGGAGGCCGAGTAATAGATGTTCTAGCTCGAGACAAGGGTGCCTGTTTCTTGATTGGAGCCTCCCTCTATGGCACTGAGGTAGATCCATCACTTGCTACCAAAGTCAAGGTTCCTGTGTTGCTCATCTCAGGGGACGATGACCCACTTTGTCCAGTGAGTGTCATGAAGGATTTGGAGAAAGGGATGGGAGAAGGTTCCAAGTTAGTGATATTTGAAGGAAGAGGCCATGGCTTTGTTCACAATCCTGGATCTCCAGAAGAAGATGATGATGCTGAACAGGCTTTTCTAATGCTTAGAAATTGGCTACATCACGGCTTGATTGCAACTAATAATTTAGCTAACTGCCATGGGTAA

Protein sequence

MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSVKGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSIRDFAYRLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGLIATNNLANCHG

Homology

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BLAST of MELO3C022228.jh1.t2 vs. NCBI nr
Match: XP_008459689.1 (PREDICTED: uncharacterized protein LOC103498731 [Cucumis melo] >KAA0039216.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa] >TYK00404.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 624 bits (1609), Expect = 3.31e-225
Identity = 307/327 (93.88%), Postives = 309/327 (94.50%), Query Frame = 0


Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60
           MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60

Query: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120
           KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI
Sbjct: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120

Query: 121 RDFAYRLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEKPPAQHSAKQEMQRIAE 180
           RDFAYRLACNGF                  VLLPDLVNEDPWEKPPAQHSAKQEMQRIAE
Sbjct: 121 RDFAYRLACNGFN-----------------VLLPDLVNEDPWEKPPAQHSAKQEMQRIAE 180

Query: 181 DIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPS 240
           DIGTVTKWLTDEFSAAGLSRKLGIIGFG+GGGRVIDVLARDKGACFLIGASLYGTEVDPS
Sbjct: 181 DIGTVTKWLTDEFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPS 240

Query: 241 LATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDD 300
           LATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDD
Sbjct: 241 LATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDD 300

Query: 301 AEQAFLMLRNWLHHGLIATNNLANCHG 327
           AEQAFLMLRNWLHHGL+ATNNLANCHG
Sbjct: 301 AEQAFLMLRNWLHHGLVATNNLANCHG 310

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BLAST of MELO3C022228.jh1.t2 vs. NCBI nr
Match: XP_004141569.1 (uncharacterized protein LOC101210518 [Cucumis sativus] >KGN52723.1 hypothetical protein Csa_008898 [Cucumis sativus])

HSP 1 Score: 553 bits (1425), Expect = 3.64e-197
Identity = 279/328 (85.06%), Postives = 294/328 (89.63%), Query Frame = 0


Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60
           MGFASAGAQLISLPPTGSSSRRV KP QPPY +FAYPLPS KY+N G W+WKGKLRY SV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRY-SV 60

Query: 61  KGRVKDDDDD-ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSS 120
           +GR+KDDDDD ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQ SS
Sbjct: 61  EGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSS 120

Query: 121 IRDFAYRLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEKPPAQHSAKQEMQRIA 180
           IRDFAYRLAC GF                  VLLPDLV+ED WEKPPA+H AKQEMQRIA
Sbjct: 121 IRDFAYRLACIGFN-----------------VLLPDLVHEDVWEKPPAEHCAKQEMQRIA 180

Query: 181 EDIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDP 240
           EDIGTVTKWLT+EFSAAGLS KLGIIGFGYGGGRVIDVLARD+G CFLIGAS+YGT+VDP
Sbjct: 181 EDIGTVTKWLTEEFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDP 240

Query: 241 SLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDD 300
           SLATKVKVPVLLISGD+DPLCPVSVMKDLEKG+GEGSKLVIF GRGHGFVHNPGSPEEDD
Sbjct: 241 SLATKVKVPVLLISGDNDPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDD 300

Query: 301 DAEQAFLMLRNWLHHGLIATNNLANCHG 327
           DAE+AFLMLRNWLHHGL+A NNLANCHG
Sbjct: 301 DAEKAFLMLRNWLHHGLVAINNLANCHG 310

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BLAST of MELO3C022228.jh1.t2 vs. NCBI nr
Match: XP_038890567.1 (hydrolase tropI [Benincasa hispida])

HSP 1 Score: 474 bits (1219), Expect = 8.24e-166
Identity = 251/329 (76.29%), Postives = 267/329 (81.16%), Query Frame = 0


Query: 1   MGFASAGAQLISLPPTGSSS--RRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRY- 60
           MG ASAGAQLISLPPTGSSS  RR+  P Q PY +F  PLP  K  N  DWKWKGK    
Sbjct: 1   MGLASAGAQLISLPPTGSSSCRRRIGTPFQLPYFRFTSPLPFRKCGNRSDWKWKGKREKK 60

Query: 61  -----YSVKGRVKDDDDDA-CELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120
                YSV+G   DDDDDA CELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD
Sbjct: 61  GGKVCYSVEGVEDDDDDDAACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120

Query: 121 LFAFQDSSIRDFAYRLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEKPPAQHSA 180
           LFAFQ SSIRDFAYRLACNGF                  VLLPDL++ D W+K     S 
Sbjct: 121 LFAFQHSSIRDFAYRLACNGFN-----------------VLLPDLIHGDSWKK----QST 180

Query: 181 KQEMQRIAEDIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGAS 240
           K++MQRIAEDIGTVTKWL DEFSAAGLSRKLGI+GFGYGGG+VI+VLARD+GACF IGAS
Sbjct: 181 KRDMQRIAEDIGTVTKWLVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGAS 240

Query: 241 LYGTEVDPSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHN 300
           LYGTEVDPSLATKVKVPVLLISGD+DPLCPVSVMKDLEK MGEGSKLVIF GRGH FVHN
Sbjct: 241 LYGTEVDPSLATKVKVPVLLISGDNDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHN 300

Query: 301 PGSPEEDDDAEQAFLMLRNWLHHGLIATN 320
           P SPEED+DAEQAFLM+RNWLHHGL+ATN
Sbjct: 301 PRSPEEDEDAEQAFLMIRNWLHHGLVATN 308

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BLAST of MELO3C022228.jh1.t2 vs. NCBI nr
Match: XP_023536049.1 (carboxymethylenebutenolidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 408 bits (1049), Expect = 3.90e-140
Identity = 226/327 (69.11%), Postives = 252/327 (77.06%), Query Frame = 0


Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWK-----GKL 60
           MG A+AGAQ IS PPTG S RR+  P QP + +FA+P+     NN G  K K     GKL
Sbjct: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPSF-RFAFPIS----NNIGQLKGKREKKGGKL 60

Query: 61  RYYSVKGRVKD--DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLF 120
             YS +G VKD  D+++ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DLF
Sbjct: 61  -CYSAEG-VKDEIDEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLF 120

Query: 121 AFQDSSIRDFAYRLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEKPPAQHSAKQ 180
           AFQDSSIRDFAYRLACNGF                  VLLP++ + D  E+         
Sbjct: 121 AFQDSSIRDFAYRLACNGFN-----------------VLLPNIFHGDSSEE--------- 180

Query: 181 EMQRIAEDIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLY 240
             Q+I EDIG  TKWL DEFSAAG+SRKLGI+GFGYGGGRVIDVLARD+G+ F IGASLY
Sbjct: 181 --QKIVEDIGAATKWLVDEFSAAGISRKLGIVGFGYGGGRVIDVLARDQGSYFSIGASLY 240

Query: 241 GTEVDPSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPG 300
           GTEVDPSLATKV+VPVLLISGD+D LCPVSVMKDLEKGMG+GSKLVIF GRGHGFVH PG
Sbjct: 241 GTEVDPSLATKVEVPVLLISGDNDTLCPVSVMKDLEKGMGDGSKLVIFGGRGHGFVHKPG 292

Query: 301 SPEEDDDAEQAFLMLRNWLHHGLIATN 320
           SPEED+DAEQAFLMLRNWLH GL+ATN
Sbjct: 301 SPEEDEDAEQAFLMLRNWLHDGLVATN 292

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BLAST of MELO3C022228.jh1.t2 vs. NCBI nr
Match: XP_022925419.1 (carboxymethylenebutenolidase homolog [Cucurbita moschata] >KAG6592400.1 Carboxymethylenebutenolidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 405 bits (1040), Expect = 9.11e-139
Identity = 220/326 (67.48%), Postives = 251/326 (76.99%), Query Frame = 0


Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLR---- 60
           MG A+AGAQ IS PPTG S RR+  P QP + +FA+P+     N+ G  K K +++    
Sbjct: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPSF-RFAFPIS----NSIGQLKGKREIKGGKV 60

Query: 61  YYSVKGRVKDD--DDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFA 120
            YS +G VKD+  +++ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DLFA
Sbjct: 61  CYSAEG-VKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFA 120

Query: 121 FQDSSIRDFAYRLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEKPPAQHSAKQE 180
           FQDSS+RDFAYRLACNGF                  VLLPD+ + D  E+          
Sbjct: 121 FQDSSVRDFAYRLACNGFN-----------------VLLPDVFHGDSCEE---------- 180

Query: 181 MQRIAEDIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYG 240
            Q+  EDI   TKWL DEFSAAG+SRKLGI+GFGYGGGRVIDVLA+DKG+ F IGASLYG
Sbjct: 181 -QKTVEDIAAATKWLVDEFSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYG 240

Query: 241 TEVDPSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGS 300
           TEV+PSLATKV+VPVLLISGD+DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGS
Sbjct: 241 TEVNPSLATKVEVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGS 292

Query: 301 PEEDDDAEQAFLMLRNWLHHGLIATN 320
           PEED+DAEQAFLMLRNWLH GL+ATN
Sbjct: 301 PEEDEDAEQAFLMLRNWLHDGLVATN 292

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BLAST of MELO3C022228.jh1.t2 vs. ExPASy TrEMBL
Match: A0A5A7TB03 (Carboxymethylenebutenolidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00030 PE=4 SV=1)

HSP 1 Score: 624 bits (1609), Expect = 1.60e-225
Identity = 307/327 (93.88%), Postives = 309/327 (94.50%), Query Frame = 0


Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60
           MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60

Query: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120
           KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI
Sbjct: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120

Query: 121 RDFAYRLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEKPPAQHSAKQEMQRIAE 180
           RDFAYRLACNGF                  VLLPDLVNEDPWEKPPAQHSAKQEMQRIAE
Sbjct: 121 RDFAYRLACNGFN-----------------VLLPDLVNEDPWEKPPAQHSAKQEMQRIAE 180

Query: 181 DIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPS 240
           DIGTVTKWLTDEFSAAGLSRKLGIIGFG+GGGRVIDVLARDKGACFLIGASLYGTEVDPS
Sbjct: 181 DIGTVTKWLTDEFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPS 240

Query: 241 LATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDD 300
           LATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDD
Sbjct: 241 LATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDD 300

Query: 301 AEQAFLMLRNWLHHGLIATNNLANCHG 327
           AEQAFLMLRNWLHHGL+ATNNLANCHG
Sbjct: 301 AEQAFLMLRNWLHHGLVATNNLANCHG 310

[Close]

BLAST of MELO3C022228.jh1.t2 vs. ExPASy TrEMBL
Match: A0A1S3CB89 (uncharacterized protein LOC103498731 OS=Cucumis melo OX=3656 GN=LOC103498731 PE=4 SV=1)

HSP 1 Score: 624 bits (1609), Expect = 1.60e-225
Identity = 307/327 (93.88%), Postives = 309/327 (94.50%), Query Frame = 0


Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60
           MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60

Query: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120
           KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI
Sbjct: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120

Query: 121 RDFAYRLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEKPPAQHSAKQEMQRIAE 180
           RDFAYRLACNGF                  VLLPDLVNEDPWEKPPAQHSAKQEMQRIAE
Sbjct: 121 RDFAYRLACNGFN-----------------VLLPDLVNEDPWEKPPAQHSAKQEMQRIAE 180

Query: 181 DIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPS 240
           DIGTVTKWLTDEFSAAGLSRKLGIIGFG+GGGRVIDVLARDKGACFLIGASLYGTEVDPS
Sbjct: 181 DIGTVTKWLTDEFSAAGLSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPS 240

Query: 241 LATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDD 300
           LATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDD
Sbjct: 241 LATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDD 300

Query: 301 AEQAFLMLRNWLHHGLIATNNLANCHG 327
           AEQAFLMLRNWLHHGL+ATNNLANCHG
Sbjct: 301 AEQAFLMLRNWLHHGLVATNNLANCHG 310

[Close]

BLAST of MELO3C022228.jh1.t2 vs. ExPASy TrEMBL
Match: A0A0A0KT04 (DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652280 PE=4 SV=1)

HSP 1 Score: 553 bits (1425), Expect = 1.76e-197
Identity = 279/328 (85.06%), Postives = 294/328 (89.63%), Query Frame = 0


Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60
           MGFASAGAQLISLPPTGSSSRRV KP QPPY +FAYPLPS KY+N G W+WKGKLRY SV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRY-SV 60

Query: 61  KGRVKDDDDD-ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSS 120
           +GR+KDDDDD ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQ SS
Sbjct: 61  EGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSS 120

Query: 121 IRDFAYRLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEKPPAQHSAKQEMQRIA 180
           IRDFAYRLAC GF                  VLLPDLV+ED WEKPPA+H AKQEMQRIA
Sbjct: 121 IRDFAYRLACIGFN-----------------VLLPDLVHEDVWEKPPAEHCAKQEMQRIA 180

Query: 181 EDIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDP 240
           EDIGTVTKWLT+EFSAAGLS KLGIIGFGYGGGRVIDVLARD+G CFLIGAS+YGT+VDP
Sbjct: 181 EDIGTVTKWLTEEFSAAGLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDP 240

Query: 241 SLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDD 300
           SLATKVKVPVLLISGD+DPLCPVSVMKDLEKG+GEGSKLVIF GRGHGFVHNPGSPEEDD
Sbjct: 241 SLATKVKVPVLLISGDNDPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDD 300

Query: 301 DAEQAFLMLRNWLHHGLIATNNLANCHG 327
           DAE+AFLMLRNWLHHGL+A NNLANCHG
Sbjct: 301 DAEKAFLMLRNWLHHGLVAINNLANCHG 310

[Close]

BLAST of MELO3C022228.jh1.t2 vs. ExPASy TrEMBL
Match: A0A6J1EC40 (carboxymethylenebutenolidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111432716 PE=4 SV=1)

HSP 1 Score: 405 bits (1040), Expect = 4.41e-139
Identity = 220/326 (67.48%), Postives = 251/326 (76.99%), Query Frame = 0


Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLR---- 60
           MG A+AGAQ IS PPTG S RR+  P QP + +FA+P+     N+ G  K K +++    
Sbjct: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPSF-RFAFPIS----NSIGQLKGKREIKGGKV 60

Query: 61  YYSVKGRVKDD--DDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFA 120
            YS +G VKD+  +++ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DLFA
Sbjct: 61  CYSAEG-VKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFA 120

Query: 121 FQDSSIRDFAYRLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEKPPAQHSAKQE 180
           FQDSS+RDFAYRLACNGF                  VLLPD+ + D  E+          
Sbjct: 121 FQDSSVRDFAYRLACNGFN-----------------VLLPDVFHGDSCEE---------- 180

Query: 181 MQRIAEDIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYG 240
            Q+  EDI   TKWL DEFSAAG+SRKLGI+GFGYGGGRVIDVLA+DKG+ F IGASLYG
Sbjct: 181 -QKTVEDIAAATKWLVDEFSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYG 240

Query: 241 TEVDPSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGS 300
           TEV+PSLATKV+VPVLLISGD+DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGS
Sbjct: 241 TEVNPSLATKVEVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGS 292

Query: 301 PEEDDDAEQAFLMLRNWLHHGLIATN 320
           PEED+DAEQAFLMLRNWLH GL+ATN
Sbjct: 301 PEEDEDAEQAFLMLRNWLHDGLVATN 292

[Close]

BLAST of MELO3C022228.jh1.t2 vs. ExPASy TrEMBL
Match: A0A6J1I908 (carboxymethylenebutenolidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472595 PE=4 SV=1)

HSP 1 Score: 397 bits (1019), Expect = 6.87e-136
Identity = 220/327 (67.28%), Postives = 249/327 (76.15%), Query Frame = 0


Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWK-----GKL 60
           MG ASAGAQ IS PPTG S RR+  P QP + + A+P+     NN G  K K     GK+
Sbjct: 1   MGSASAGAQWISAPPTGRSGRRMGIPFQPSF-RCAFPIS----NNIGQLKGKREKKGGKV 60

Query: 61  RYYSVKGRVKD--DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLF 120
             YS +G VKD  D ++ACELV+GMELSIGE+ HHSI A+LFKAVKNNNGTAILLL+DLF
Sbjct: 61  -CYSAEG-VKDEIDAEEACELVSGMELSIGEEAHHSITAHLFKAVKNNNGTAILLLTDLF 120

Query: 121 AFQDSSIRDFAYRLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEKPPAQHSAKQ 180
           +FQDSS+RDFAYRLACNGF                  VLLPD+ + D  E+         
Sbjct: 121 SFQDSSVRDFAYRLACNGFN-----------------VLLPDVFHGDSCEE--------- 180

Query: 181 EMQRIAEDIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLY 240
             Q+  EDIG  TKWL DEF AAG+SRKLGI+GFGYGGGRVIDVLA+D+G+ F IGASLY
Sbjct: 181 --QKAVEDIGAATKWLVDEFLAAGISRKLGIVGFGYGGGRVIDVLAQDQGSYFSIGASLY 240

Query: 241 GTEVDPSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPG 300
           GTEV+PSLATKV+VPVLLISGD+DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PG
Sbjct: 241 GTEVNPSLATKVEVPVLLISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPG 292

Query: 301 SPEEDDDAEQAFLMLRNWLHHGLIATN 320
           SPEED+DAEQAFLMLRNWLH GL+ATN
Sbjct: 301 SPEEDEDAEQAFLMLRNWLHDGLVATN 292

[Close]

BLAST of MELO3C022228.jh1.t2 vs. TAIR 10
Match: AT1G35420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 273.1 bits (697), Expect = 2.9e-73
Identity = 133/257 (51.75%), Postives = 178/257 (69.26%), Query Frame = 0


Query: 66  DDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSIRDFAY 125
           D++++ACELVNG E+S+       +  YL  AVKNNNGT +LLLSD+F FQDS+ RDFAY
Sbjct: 76  DEEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFAY 135

Query: 126 RLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEK--PPAQHS---AKQEMQRIAE 185
           R+ACNG+                  VL+PDL   DPW K  P +++       +  RI +
Sbjct: 136 RVACNGYN-----------------VLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQ 195

Query: 186 DIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPS 245
           D  T TKW+ +EF+AAG+S+KLG++GF +GGGRV+DVLA D+   F  G S YGT +D +
Sbjct: 196 DTTTFTKWMVEEFAAAGISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSA 255

Query: 246 LATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDD 305
           +A  VKVPVL I+GD DPLC V  + ++E+ +GEGSK+V++EGRGHGFVH P +PE+D D
Sbjct: 256 VAGDVKVPVLFIAGDRDPLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRD 310

Query: 306 AEQAFLMLRNWLHHGLI 318
           AE+AF ++RNWLHH LI
Sbjct: 316 AEEAFALMRNWLHHHLI 310

[Close]

BLAST of MELO3C022228.jh1.t2 vs. TAIR 10
Match: AT1G35420.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 273.1 bits (697), Expect = 2.9e-73
Identity = 133/257 (51.75%), Postives = 178/257 (69.26%), Query Frame = 0


Query: 66  DDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSIRDFAY 125
           D++++ACELVNG E+S+       +  YL  AVKNNNGT +LLLSD+F FQDS+ RDFAY
Sbjct: 81  DEEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFAY 140

Query: 126 RLACNGFKYAALTFTFNSNFVSNNIVLLPDLVNEDPWEK--PPAQHS---AKQEMQRIAE 185
           R+ACNG+                  VL+PDL   DPW K  P +++       +  RI +
Sbjct: 141 RVACNGYN-----------------VLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQ 200

Query: 186 DIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPS 245
           D  T TKW+ +EF+AAG+S+KLG++GF +GGGRV+DVLA D+   F  G S YGT +D +
Sbjct: 201 DTTTFTKWMVEEFAAAGISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSA 260

Query: 246 LATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDD 305
           +A  VKVPVL I+GD DPLC V  + ++E+ +GEGSK+V++EGRGHGFVH P +PE+D D
Sbjct: 261 VAGDVKVPVLFIAGDRDPLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRD 315

Query: 306 AEQAFLMLRNWLHHGLI 318
           AE+AF ++RNWLHH LI
Sbjct: 321 AEEAFALMRNWLHHHLI 315

The following BLAST results are available for this feature:

BLAST of MELO3C022228.jh1.t2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459689.1	3.31e-225	93.88	PREDICTED: uncharacterized protein LOC103498731 [Cucumis melo] >KAA0039216.1 car...	[more]
XP_004141569.1	3.64e-197	85.06	uncharacterized protein LOC101210518 [Cucumis sativus] >KGN52723.1 hypothetical ...	[more]
XP_038890567.1	8.24e-166	76.29	hydrolase tropI [Benincasa hispida]	[more]
XP_023536049.1	3.90e-140	69.11	carboxymethylenebutenolidase homolog [Cucurbita pepo subsp. pepo]	[more]
XP_022925419.1	9.11e-139	67.48	carboxymethylenebutenolidase homolog [Cucurbita moschata] >KAG6592400.1 Carboxym...	[more]

BLAST of MELO3C022228.jh1.t2 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MELO3C022228.jh1.t2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TB03	1.60e-225	93.88	Carboxymethylenebutenolidase-like protein OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3CB89	1.60e-225	93.88	uncharacterized protein LOC103498731 OS=Cucumis melo OX=3656 GN=LOC103498731 PE=...	[more]
A0A0A0KT04	1.76e-197	85.06	DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652280 PE=4 SV...	[more]
A0A6J1EC40	4.41e-139	67.48	carboxymethylenebutenolidase homolog OS=Cucurbita moschata OX=3662 GN=LOC1114327...	[more]
A0A6J1I908	6.87e-136	67.28	carboxymethylenebutenolidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472595...	[more]

BLAST of MELO3C022228.jh1.t2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G35420.1	2.9e-73	51.75	alpha/beta-Hydrolases superfamily protein	[more]
AT1G35420.2	2.9e-73	51.75	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002925	Dienelactone hydrolase	PFAM	PF01738	DLH	coord: 91..314 e-value: 1.5E-13 score: 50.8
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 79..314 e-value: 1.7E-28 score: 101.7
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 87..314
None	No IPR available	PANTHER	PTHR17630	DIENELACTONE HYDROLASE	coord: 47..318
None	No IPR available	PANTHER	PTHR17630:SF88	DIENELACTONE HYDROLASE FAMILY PROTEIN	coord: 47..318

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MELO3C022228.jh1	MELO3C022228.jh1	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MELO3C022228.jh1.t2.exon	MELO3C022228.jh1.t2.exon	exon
MELO3C022228.jh1.t2.exon	MELO3C022228.jh1.t2.exon_2	exon
MELO3C022228.jh1.t2.exon	MELO3C022228.jh1.t2.exon_3	exon

The following stop_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MELO3C022228.jh1.t2.stop_codon	MELO3C022228.jh1.t2.stop_codon	stop_codon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MELO3C022228.jh1.t2.cds	MELO3C022228.jh1.t2.cds	CDS
MELO3C022228.jh1.t2.cds	MELO3C022228.jh1.t2.cds_2	CDS
MELO3C022228.jh1.t2.cds	MELO3C022228.jh1.t2.cds_3	CDS

The following start_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MELO3C022228.jh1.t2.start_codon	MELO3C022228.jh1.t2.start_codon	start_codon

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MELO3C022228.jh1.t2	MELO3C022228.jh1.t2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity