MELO3C022228.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C022228.jh1 vs. NCBI nr
Match: XP_008459689.1 (PREDICTED: uncharacterized protein LOC103498731 [Cucumis melo] >KAA0039216.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa] >TYK00404.1 carboxymethylenebutenolidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 638 bits (1646), Expect = 3.80e-231
Identity = 308/310 (99.35%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60
           MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60

Query: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120
           KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI
Sbjct: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120

Query: 121 RDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAG 180
           RDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAG
Sbjct: 121 RDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAG 180

Query: 181 LSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD 240
           LSRKLGIIGFG+GGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD
Sbjct: 181 LSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD 240

Query: 241 PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGLI 300
           PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGL+
Sbjct: 241 PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGLV 300

Query: 301 ATNNLANCHG 310
           ATNNLANCHG
Sbjct: 301 ATNNLANCHG 310

BLAST of MELO3C022228.jh1 vs. NCBI nr
Match: XP_004141569.1 (uncharacterized protein LOC101210518 [Cucumis sativus] >KGN52723.1 hypothetical protein Csa_008898 [Cucumis sativus])

HSP 1 Score: 567 bits (1462), Expect = 4.25e-203
Identity = 280/311 (90.03%), Postives = 295/311 (94.86%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60
           MGFASAGAQLISLPPTGSSSRRV KP QPPY +FAYPLPS KY+N G W+WKGKLRY SV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRY-SV 60

Query: 61  KGRVKDDDDD-ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSS 120
           +GR+KDDDDD ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQ SS
Sbjct: 61  EGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSS 120

Query: 121 IRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAA 180
           IRDFAYRLAC GFNVLLPDLV+ED WEKPPA+H AKQEMQRIAEDIGTVTKWLT+EFSAA
Sbjct: 121 IRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAA 180

Query: 181 GLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDD 240
           GLS KLGIIGFGYGGGRVIDVLARD+G CFLIGAS+YGT+VDPSLATKVKVPVLLISGD+
Sbjct: 181 GLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDN 240

Query: 241 DPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGL 300
           DPLCPVSVMKDLEKG+GEGSKLVIF GRGHGFVHNPGSPEEDDDAE+AFLMLRNWLHHGL
Sbjct: 241 DPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGL 300

Query: 301 IATNNLANCHG 310
           +A NNLANCHG
Sbjct: 301 VAINNLANCHG 310

BLAST of MELO3C022228.jh1 vs. NCBI nr
Match: XP_038890567.1 (hydrolase tropI [Benincasa hispida])

HSP 1 Score: 488 bits (1256), Expect = 9.85e-172
Identity = 252/312 (80.77%), Postives = 268/312 (85.90%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSS--RRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRY- 60
           MG ASAGAQLISLPPTGSSS  RR+  P Q PY +F  PLP  K  N  DWKWKGK    
Sbjct: 1   MGLASAGAQLISLPPTGSSSCRRRIGTPFQLPYFRFTSPLPFRKCGNRSDWKWKGKREKK 60

Query: 61  -----YSVKGRVKDDDDDA-CELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120
                YSV+G   DDDDDA CELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD
Sbjct: 61  GGKVCYSVEGVEDDDDDDAACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSD 120

Query: 121 LFAFQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKW 180
           LFAFQ SSIRDFAYRLACNGFNVLLPDL++ D W+K     S K++MQRIAEDIGTVTKW
Sbjct: 121 LFAFQHSSIRDFAYRLACNGFNVLLPDLIHGDSWKK----QSTKRDMQRIAEDIGTVTKW 180

Query: 181 LTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVP 240
           L DEFSAAGLSRKLGI+GFGYGGG+VI+VLARD+GACF IGASLYGTEVDPSLATKVKVP
Sbjct: 181 LVDEFSAAGLSRKLGIMGFGYGGGQVIEVLARDEGACFCIGASLYGTEVDPSLATKVKVP 240

Query: 241 VLLISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLML 300
           VLLISGD+DPLCPVSVMKDLEK MGEGSKLVIF GRGH FVHNP SPEED+DAEQAFLM+
Sbjct: 241 VLLISGDNDPLCPVSVMKDLEKRMGEGSKLVIFGGRGHDFVHNPRSPEEDEDAEQAFLMI 300

Query: 301 RNWLHHGLIATN 303
           RNWLHHGL+ATN
Sbjct: 301 RNWLHHGLVATN 308

BLAST of MELO3C022228.jh1 vs. NCBI nr
Match: XP_023536049.1 (carboxymethylenebutenolidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 423 bits (1087), Expect = 3.38e-146
Identity = 227/310 (73.23%), Postives = 251/310 (80.97%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWK-----GKL 60
           MG A+AGAQ IS PPTG S RR+  P QP + +FA+P+     NN G  K K     GKL
Sbjct: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPSF-RFAFPIS----NNIGQLKGKREKKGGKL 60

Query: 61  RYYSVKGRVKD--DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLF 120
             YS +G VKD  D+++ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DLF
Sbjct: 61  -CYSAEG-VKDEIDEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLF 120

Query: 121 AFQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLT 180
           AFQDSSIRDFAYRLACNGFNVLLP++            H    E Q+I EDIG  TKWL 
Sbjct: 121 AFQDSSIRDFAYRLACNGFNVLLPNIF-----------HGDSSEEQKIVEDIGAATKWLV 180

Query: 181 DEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVL 240
           DEFSAAG+SRKLGI+GFGYGGGRVIDVLARD+G+ F IGASLYGTEVDPSLATKV+VPVL
Sbjct: 181 DEFSAAGISRKLGIVGFGYGGGRVIDVLARDQGSYFSIGASLYGTEVDPSLATKVEVPVL 240

Query: 241 LISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRN 300
           LISGD+D LCPVSVMKDLEKGMG+GSKLVIF GRGHGFVH PGSPEED+DAEQAFLMLRN
Sbjct: 241 LISGDNDTLCPVSVMKDLEKGMGDGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRN 292

Query: 301 WLHHGLIATN 303
           WLH GL+ATN
Sbjct: 301 WLHDGLVATN 292

BLAST of MELO3C022228.jh1 vs. NCBI nr
Match: XP_022925419.1 (carboxymethylenebutenolidase homolog [Cucurbita moschata] >KAG6592400.1 Carboxymethylenebutenolidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 419 bits (1077), Expect = 1.12e-144
Identity = 221/309 (71.52%), Postives = 252/309 (81.55%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLR---- 60
           MG A+AGAQ IS PPTG S RR+  P QP + +FA+P+     N+ G  K K +++    
Sbjct: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPSF-RFAFPIS----NSIGQLKGKREIKGGKV 60

Query: 61  YYSVKGRVKDD--DDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFA 120
            YS +G VKD+  +++ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DLFA
Sbjct: 61  CYSAEG-VKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFA 120

Query: 121 FQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTD 180
           FQDSS+RDFAYRLACNGFNVLLPD+ + D  E+           Q+  EDI   TKWL D
Sbjct: 121 FQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEE-----------QKTVEDIAAATKWLVD 180

Query: 181 EFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLL 240
           EFSAAG+SRKLGI+GFGYGGGRVIDVLA+DKG+ F IGASLYGTEV+PSLATKV+VPVLL
Sbjct: 181 EFSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLL 240

Query: 241 ISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNW 300
           ISGD+DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGSPEED+DAEQAFLMLRNW
Sbjct: 241 ISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNW 292

Query: 301 LHHGLIATN 303
           LH GL+ATN
Sbjct: 301 LHDGLVATN 292

BLAST of MELO3C022228.jh1 vs. ExPASy Swiss-Prot
Match: Q96DG6 (Carboxymethylenebutenolidase homolog OS=Homo sapiens OX=9606 GN=CMBL PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.7e-14
Identity = 64/221 (28.96%), Postives = 109/221 (49.32%), Query Frame = 0

Query: 90  IPAYLFKAVKNNNGTAILLLSDLFAFQDSSIRDFAYRLACNGFNVLLPD-LVNEDPWEKP 149
           I AY+ K+   + G A++++ D+F +Q  + R  A  ++ NG+  ++PD  V ++PW+ P
Sbjct: 30  IKAYVTKS-PVDAGKAVIVIQDIFGWQLPNTRYIADMISGNGYTTIVPDFFVGQEPWD-P 89

Query: 150 PAQHSAKQE------MQRIAEDIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLA 209
               S   E       Q+I  +I  + K+L  +  A    +K+GI+GF +GG  V  ++ 
Sbjct: 90  SGDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHA----QKIGIVGFCWGGTAVHHLMM 149

Query: 210 RDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSK-- 269
             K + F  G S+YG   D      +K P L I  ++D + P+  +  L + + E  K  
Sbjct: 150 --KYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVE 209

Query: 270 --LVIFEGRGHGFVHNPG---SPEEDDDAEQAFLMLRNWLH 297
             +  F G+ HGFVH      SP +    ++A   L  WL+
Sbjct: 210 YQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLN 242

BLAST of MELO3C022228.jh1 vs. ExPASy Swiss-Prot
Match: Q5RBU3 (Carboxymethylenebutenolidase homolog OS=Pongo abelii OX=9601 GN=CMBL PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.8e-14
Identity = 64/221 (28.96%), Postives = 109/221 (49.32%), Query Frame = 0

Query: 90  IPAYLFKAVKNNNGTAILLLSDLFAFQDSSIRDFAYRLACNGFNVLLPD-LVNEDPWEKP 149
           I AY+ K+   + G A++++ D+F +Q  + R  A  ++ NG+  ++PD  V ++PW+ P
Sbjct: 30  IKAYVTKS-PVDAGKAVIVIQDIFGWQLPNTRYMADMISGNGYTTIVPDFFVGQEPWD-P 89

Query: 150 PAQHSAKQE------MQRIAEDIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLA 209
               S   E       Q+I  +I  + K+L  +  A    +K+GI+GF +GG  V  ++ 
Sbjct: 90  SGDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHA----QKIGIVGFCWGGIAVHHLMM 149

Query: 210 RDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGEGSK-- 269
             K + F  G S+YG   D      +K P L I  ++D + P+  +  L + + E  K  
Sbjct: 150 --KYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVE 209

Query: 270 --LVIFEGRGHGFVHNPG---SPEEDDDAEQAFLMLRNWLH 297
             +  F G+ HGFVH      SP +    ++A   L  WL+
Sbjct: 210 YQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLN 242

BLAST of MELO3C022228.jh1 vs. ExPASy Swiss-Prot
Match: Q8R1G2 (Carboxymethylenebutenolidase homolog OS=Mus musculus OX=10090 GN=Cmbl PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.4e-13
Identity = 64/248 (25.81%), Postives = 115/248 (46.37%), Query Frame = 0

Query: 67  DDDDACELVNGMELSIGEQPH----HSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSIRD 126
           ++ + C    G +L  G   H      I AY+ ++   + G A++++ D+F +Q  + R 
Sbjct: 3   NEANPCPCDIGHKLEYGGMGHEVQVEHIKAYVTRS-PVDAGKAVIVVQDIFGWQLPNTRY 62

Query: 127 FAYRLACNGFNVLLPD-LVNEDPWEKPPAQHSA------KQEMQRIAEDIGTVTKWLTDE 186
            A  +A NG+  ++PD  V ++PW+ P    S        +  +++  ++  V ++L  +
Sbjct: 63  MADMIARNGYTTIVPDFFVGQEPWD-PAGDWSTFPAWLKSRNARKVNREVDAVLRYLRQQ 122

Query: 187 FSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLI 246
             A    +K+GI+GF +GG  V  V+          G S+YG   D      +K P L I
Sbjct: 123 CHA----QKIGIVGFCWGGVVVHQVMTAYPD--IRAGVSVYGIIRDSEDVYNLKNPTLFI 182

Query: 247 SGDDDPLCPVSVMKDLEKGMGE----GSKLVIFEGRGHGFVHNPG---SPEEDDDAEQAF 297
             ++D + P+  +  L + + E      ++  F G+ HGFVH      SP +    E+A 
Sbjct: 183 FAENDTVIPLEQVSTLTQKLKEHCIVNYQVKTFSGQTHGFVHRKREDCSPADKPYIEEAR 242

BLAST of MELO3C022228.jh1 vs. ExPASy Swiss-Prot
Match: Q7TP52 (Carboxymethylenebutenolidase homolog OS=Rattus norvegicus OX=10116 GN=Cmbl PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 7.0e-13
Identity = 58/208 (27.88%), Postives = 100/208 (48.08%), Query Frame = 0

Query: 103 GTAILLLSDLFAFQDSSIRDFAYRLACNGFNVLLPD-LVNEDPWEKPPAQHSA------K 162
           G A++++ D+F +Q S+ R  A  +A NG+  ++PD  V ++PW+ P    S        
Sbjct: 42  GKAVIVVQDIFGWQLSNTRYMADMIAGNGYTTIVPDFFVGQEPWD-PAGDWSTFPEWLKS 101

Query: 163 QEMQRIAEDIGTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASL 222
           +  ++I  ++  V ++L  +  A    +K+GI+GF +GG  V  V+          G S+
Sbjct: 102 RNARKINREVDAVLRYLKQQCHA----QKIGIVGFCWGGIVVHHVMTTYPEV--RAGVSV 161

Query: 223 YGTEVDPSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGE----GSKLVIFEGRGHGF 282
           YG   D      +K P L I  ++D + P+  +  L + + E      ++  F G+ HGF
Sbjct: 162 YGIIRDSEDVYNLKNPTLFIFAENDAVIPLEQVSILIQKLKEHCIVNYQVKTFSGQTHGF 221

Query: 283 VHNPG---SPEEDDDAEQAFLMLRNWLH 297
           VH      SP +    E+A   L  WL+
Sbjct: 222 VHRKREDCSPADKPYIEEARRNLIEWLN 242

BLAST of MELO3C022228.jh1 vs. ExPASy Swiss-Prot
Match: A0A2V5GSC6 (Hydrolase pyvD OS=Aspergillus violaceofuscus (strain CBS 115571) OX=1450538 GN=pyvD PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.6e-04
Identity = 50/191 (26.18%), Postives = 80/191 (41.88%), Query Frame = 0

Query: 90  IPAYLFKAVKNNNGTAILLLSDLFAFQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPP 149
           IP Y+      +   AIL LSD+F     + +  A   A NG+ V++PDL   DP    P
Sbjct: 30  IPVYVSYPPDKSTHNAILFLSDIFGLALVNSKLIADLFAANGYLVVMPDLFQGDP---VP 89

Query: 150 AQHSAK-QEMQRIAEDI-----GTVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLAR 209
             HS   Q M  +   +       VT  L +     G  R +G +G+ YGG  V+  L  
Sbjct: 90  VDHSPSFQIMDWLQGHLPPQTDPIVTATLREMRERLGCQR-IGGVGYCYGGKYVVRYLHP 149

Query: 210 DKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGE---GSK 269
            +     +G   + T ++P     ++ P+ + +   D L P     + E  + +     +
Sbjct: 150 GQ---LDVGFVAHPTFIEPDELKAIEGPLSISASAQDNLFPPEKRHESETILAQLDVPYQ 209

Query: 270 LVIFEGRGHGF 272
           + IF    HGF
Sbjct: 210 INIFSDVEHGF 213

BLAST of MELO3C022228.jh1 vs. ExPASy TrEMBL
Match: A0A5A7TB03 (Carboxymethylenebutenolidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00030 PE=4 SV=1)

HSP 1 Score: 638 bits (1646), Expect = 1.84e-231
Identity = 308/310 (99.35%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60
           MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60

Query: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120
           KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI
Sbjct: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120

Query: 121 RDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAG 180
           RDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAG
Sbjct: 121 RDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAG 180

Query: 181 LSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD 240
           LSRKLGIIGFG+GGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD
Sbjct: 181 LSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD 240

Query: 241 PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGLI 300
           PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGL+
Sbjct: 241 PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGLV 300

Query: 301 ATNNLANCHG 310
           ATNNLANCHG
Sbjct: 301 ATNNLANCHG 310

BLAST of MELO3C022228.jh1 vs. ExPASy TrEMBL
Match: A0A1S3CB89 (uncharacterized protein LOC103498731 OS=Cucumis melo OX=3656 GN=LOC103498731 PE=4 SV=1)

HSP 1 Score: 638 bits (1646), Expect = 1.84e-231
Identity = 308/310 (99.35%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60
           MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60

Query: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120
           KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI
Sbjct: 61  KGRVKDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSI 120

Query: 121 RDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAG 180
           RDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAG
Sbjct: 121 RDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAAG 180

Query: 181 LSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD 240
           LSRKLGIIGFG+GGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD
Sbjct: 181 LSRKLGIIGFGHGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD 240

Query: 241 PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGLI 300
           PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGL+
Sbjct: 241 PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGLV 300

Query: 301 ATNNLANCHG 310
           ATNNLANCHG
Sbjct: 301 ATNNLANCHG 310

BLAST of MELO3C022228.jh1 vs. ExPASy TrEMBL
Match: A0A0A0KT04 (DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652280 PE=4 SV=1)

HSP 1 Score: 567 bits (1462), Expect = 2.06e-203
Identity = 280/311 (90.03%), Postives = 295/311 (94.86%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLRYYSV 60
           MGFASAGAQLISLPPTGSSSRRV KP QPPY +FAYPLPS KY+N G W+WKGKLRY SV
Sbjct: 1   MGFASAGAQLISLPPTGSSSRRVGKPFQPPYFKFAYPLPSRKYSNMGVWRWKGKLRY-SV 60

Query: 61  KGRVKDDDDD-ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSS 120
           +GR+KDDDDD ACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQ SS
Sbjct: 61  EGRMKDDDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQHSS 120

Query: 121 IRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTDEFSAA 180
           IRDFAYRLAC GFNVLLPDLV+ED WEKPPA+H AKQEMQRIAEDIGTVTKWLT+EFSAA
Sbjct: 121 IRDFAYRLACIGFNVLLPDLVHEDVWEKPPAEHCAKQEMQRIAEDIGTVTKWLTEEFSAA 180

Query: 181 GLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDD 240
           GLS KLGIIGFGYGGGRVIDVLARD+G CFLIGAS+YGT+VDPSLATKVKVPVLLISGD+
Sbjct: 181 GLSMKLGIIGFGYGGGRVIDVLARDEGTCFLIGASIYGTQVDPSLATKVKVPVLLISGDN 240

Query: 241 DPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGL 300
           DPLCPVSVMKDLEKG+GEGSKLVIF GRGHGFVHNPGSPEEDDDAE+AFLMLRNWLHHGL
Sbjct: 241 DPLCPVSVMKDLEKGIGEGSKLVIFGGRGHGFVHNPGSPEEDDDAEKAFLMLRNWLHHGL 300

Query: 301 IATNNLANCHG 310
           +A NNLANCHG
Sbjct: 301 VAINNLANCHG 310

BLAST of MELO3C022228.jh1 vs. ExPASy TrEMBL
Match: A0A6J1EC40 (carboxymethylenebutenolidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111432716 PE=4 SV=1)

HSP 1 Score: 419 bits (1077), Expect = 5.44e-145
Identity = 221/309 (71.52%), Postives = 252/309 (81.55%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWKGKLR---- 60
           MG A+AGAQ IS PPTG S RR+  P QP + +FA+P+     N+ G  K K +++    
Sbjct: 1   MGSATAGAQWISAPPTGRSGRRMGIPFQPSF-RFAFPIS----NSIGQLKGKREIKGGKV 60

Query: 61  YYSVKGRVKDD--DDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFA 120
            YS +G VKD+  +++ACELVNGMELSIGE+ HHSI AYLFKAVKNNNGTAILLL+DLFA
Sbjct: 61  CYSAEG-VKDEIEEEEACELVNGMELSIGEEAHHSITAYLFKAVKNNNGTAILLLTDLFA 120

Query: 121 FQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLTD 180
           FQDSS+RDFAYRLACNGFNVLLPD+ + D  E+           Q+  EDI   TKWL D
Sbjct: 121 FQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEE-----------QKTVEDIAAATKWLVD 180

Query: 181 EFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLL 240
           EFSAAG+SRKLGI+GFGYGGGRVIDVLA+DKG+ F IGASLYGTEV+PSLATKV+VPVLL
Sbjct: 181 EFSAAGISRKLGIVGFGYGGGRVIDVLAQDKGSYFSIGASLYGTEVNPSLATKVEVPVLL 240

Query: 241 ISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNW 300
           ISGD+DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGSPEED+DAEQAFLMLRNW
Sbjct: 241 ISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRNW 292

Query: 301 LHHGLIATN 303
           LH GL+ATN
Sbjct: 301 LHDGLVATN 292

BLAST of MELO3C022228.jh1 vs. ExPASy TrEMBL
Match: A0A6J1I908 (carboxymethylenebutenolidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472595 PE=4 SV=1)

HSP 1 Score: 411 bits (1056), Expect = 8.50e-142
Identity = 221/310 (71.29%), Postives = 250/310 (80.65%), Query Frame = 0

Query: 1   MGFASAGAQLISLPPTGSSSRRVEKPIQPPYSQFAYPLPSWKYNNWGDWKWK-----GKL 60
           MG ASAGAQ IS PPTG S RR+  P QP + + A+P+     NN G  K K     GK+
Sbjct: 1   MGSASAGAQWISAPPTGRSGRRMGIPFQPSF-RCAFPIS----NNIGQLKGKREKKGGKV 60

Query: 61  RYYSVKGRVKD--DDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLF 120
             YS +G VKD  D ++ACELV+GMELSIGE+ HHSI A+LFKAVKNNNGTAILLL+DLF
Sbjct: 61  -CYSAEG-VKDEIDAEEACELVSGMELSIGEEAHHSITAHLFKAVKNNNGTAILLLTDLF 120

Query: 121 AFQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDIGTVTKWLT 180
           +FQDSS+RDFAYRLACNGFNVLLPD+ + D  E+           Q+  EDIG  TKWL 
Sbjct: 121 SFQDSSVRDFAYRLACNGFNVLLPDVFHGDSCEE-----------QKAVEDIGAATKWLV 180

Query: 181 DEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVL 240
           DEF AAG+SRKLGI+GFGYGGGRVIDVLA+D+G+ F IGASLYGTEV+PSLATKV+VPVL
Sbjct: 181 DEFLAAGISRKLGIVGFGYGGGRVIDVLAQDQGSYFSIGASLYGTEVNPSLATKVEVPVL 240

Query: 241 LISGDDDPLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRN 300
           LISGD+DPLCPVSVMKDLEKGMGEGSKLVIF GRGHGFVH PGSPEED+DAEQAFLMLRN
Sbjct: 241 LISGDNDPLCPVSVMKDLEKGMGEGSKLVIFGGRGHGFVHKPGSPEEDEDAEQAFLMLRN 292

Query: 301 WLHHGLIATN 303
           WLH GL+ATN
Sbjct: 301 WLHDGLVATN 292

BLAST of MELO3C022228.jh1 vs. TAIR 10
Match: AT1G35420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 8.3e-78
Identity = 134/240 (55.83%), Postives = 179/240 (74.58%), Query Frame = 0

Query: 66  DDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSIRDFAY 125
           D++++ACELVNG E+S+       +  YL  AVKNNNGT +LLLSD+F FQDS+ RDFAY
Sbjct: 76  DEEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFAY 135

Query: 126 RLACNGFNVLLPDLVNEDPWEK--PPAQHS---AKQEMQRIAEDIGTVTKWLTDEFSAAG 185
           R+ACNG+NVL+PDL   DPW K  P +++       +  RI +D  T TKW+ +EF+AAG
Sbjct: 136 RVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAAAG 195

Query: 186 LSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD 245
           +S+KLG++GF +GGGRV+DVLA D+   F  G S YGT +D ++A  VKVPVL I+GD D
Sbjct: 196 ISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGDRD 255

Query: 246 PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGLI 301
           PLC V  + ++E+ +GEGSK+V++EGRGHGFVH P +PE+D DAE+AF ++RNWLHH LI
Sbjct: 256 PLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHHLI 310

BLAST of MELO3C022228.jh1 vs. TAIR 10
Match: AT1G35420.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 8.3e-78
Identity = 134/240 (55.83%), Postives = 179/240 (74.58%), Query Frame = 0

Query: 66  DDDDDACELVNGMELSIGEQPHHSIPAYLFKAVKNNNGTAILLLSDLFAFQDSSIRDFAY 125
           D++++ACELVNG E+S+       +  YL  AVKNNNGT +LLLSD+F FQDS+ RDFAY
Sbjct: 81  DEEEEACELVNGTEVSV-----DGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFAY 140

Query: 126 RLACNGFNVLLPDLVNEDPWEK--PPAQHS---AKQEMQRIAEDIGTVTKWLTDEFSAAG 185
           R+ACNG+NVL+PDL   DPW K  P +++       +  RI +D  T TKW+ +EF+AAG
Sbjct: 141 RVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAAAG 200

Query: 186 LSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSLATKVKVPVLLISGDDD 245
           +S+KLG++GF +GGGRV+DVLA D+   F  G S YGT +D ++A  VKVPVL I+GD D
Sbjct: 201 ISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRIDSAVAGDVKVPVLFIAGDRD 260

Query: 246 PLCPVSVMKDLEKGMGEGSKLVIFEGRGHGFVHNPGSPEEDDDAEQAFLMLRNWLHHGLI 301
           PLC V  + ++E+ +GEGSK+V++EGRGHGFVH P +PE+D DAE+AF ++RNWLHH LI
Sbjct: 261 PLCEVKGLYEIEEKIGEGSKVVVYEGRGHGFVHRPETPEDDRDAEEAFALMRNWLHHHLI 315

BLAST of MELO3C022228.jh1 vs. TAIR 10
Match: AT3G23600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 52.8 bits (125), Expect = 5.9e-07
Identity = 45/196 (22.96%), Postives = 87/196 (44.39%), Query Frame = 0

Query: 106 ILLLSDLFAFQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDI 165
           +LL+SD+F F+  ++R  A ++A +GF V++PD    DP+            ++    D 
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 166 G-TVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSL 225
           G   TK + +     G++  +G  G  +G   V+++    K         L+ + V+   
Sbjct: 102 GFENTKPVLETIKNKGIT-AIGAAGMCWGAKVVVEL---SKEELIQAAVLLHPSFVNVDD 161

Query: 226 ATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGE----GSKLVIFEGRGHGFV--HNPGSP 285
               K P+ ++  + D + P +++K  E+ +       S + I     HG+   +N   P
Sbjct: 162 IKGGKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEP 221

Query: 286 EEDDDAEQAFLMLRNW 295
           E    AE+A   + +W
Sbjct: 222 EAVKAAEEAHKEMLDW 233

BLAST of MELO3C022228.jh1 vs. TAIR 10
Match: AT3G23600.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 50.8 bits (120), Expect = 2.2e-06
Identity = 45/196 (22.96%), Postives = 85/196 (43.37%), Query Frame = 0

Query: 106 ILLLSDLFAFQDSSIRDFAYRLACNGFNVLLPDLVNEDPWEKPPAQHSAKQEMQRIAEDI 165
           +LL+SD+F F+  ++R  A ++A +GF V++PD    DP+            ++    D 
Sbjct: 42  VLLISDIFGFEAPNLRALADKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDK 101

Query: 166 G-TVTKWLTDEFSAAGLSRKLGIIGFGYGGGRVIDVLARDKGACFLIGASLYGTEVDPSL 225
           G   TK + +     G++  +G  G  +G      V+   K         L+ + V+   
Sbjct: 102 GFENTKPVLETIKNKGIT-AIGAAGMCWG------VVELSKEELIQAAVLLHPSFVNVDD 161

Query: 226 ATKVKVPVLLISGDDDPLCPVSVMKDLEKGMGE----GSKLVIFEGRGHGFV--HNPGSP 285
               K P+ ++  + D + P +++K  E+ +       S + I     HG+   +N   P
Sbjct: 162 IKGGKAPIAILGAEIDQMSPPALLKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEP 221

Query: 286 EEDDDAEQAFLMLRNW 295
           E    AE+A   + +W
Sbjct: 222 EAVKAAEEAHKEMLDW 230