MC10g1040.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC10g1040.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	fatty acid desaturase 4, chloroplastic
Location	MC10: 10202765 .. 10203709 (-)
Sequence length	945
RNA-Seq Expression	MC10g1040.1
Synteny	MC10g1040.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAATCTTAGCTCAGAACAAGTTCCTTCTGAGCTCCCGAACCCGTGTTAACCCGTTGAGCCCTCCGATTCCACGTAAAATTGTCTACTGTGTAGCCAACACAGTCGACGTCGCTGCTGCTGGTGCCACCACCACCAAACCCAAGCATGCCGATGGCCAACTAGTCATTAAACGCGAGTTTGTCTCTCCTCCACTCACATTGGGCAAAGCCTCAACCTATCATTATGTTCTTAATGACCCAAGTTTACGATCGACTTGGGGCCATCGTGCGTGGGTGGCGAGTGGGTGCACCACAGTGCTCATTTCTTTGGCAAAATCAATAGTAGGTGCAGCCAGTTCACACATCTGGCTTGAGCCTACTTTAGCAGCATTGGTAGGTTACATTCTAGCAGACCTTGGATCTGGAGTTTACCATTGGGGCATTGATAACTATGGCGATGCCTCGACTCCAATCTTCGGTACCCAGATAGAAGCATTCCAAGGCCACCATAAGTGGCCATGGACAATCACGAAGCGTCAGTTTGCCAACAACTTGTATGCATTGGCACGAGCTGTGACATTCGTTGTGCTTCCCATAAACCTTGCATTTGGTGATCCCATTCTACATGGTTTTGTTAGTGTATGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCATGATAAAAAGAGCCAACTACCACCACTCGTGGTAGCAATGCAAGATGCTGGCCTGCTCGTGTCACGAATGCAACATTCCGCACATCATCGTCCACCCTATAACAACAACTATTGTATTGTAAGTGGAATATGGAATCAGCTTTTGGATGAAACAAAGGTTTTCGAGGCATTTGAGATGATCTTGTTTTTCAAGCTCGGGGTAAGACCAAGATCTTGGAGTGAGCCTAACACTGAATGGACCGAAGAGACTGAAACTGTAGAACAAACTACAGCC

mRNA sequence

ATGTCAATCTTAGCTCAGAACAAGTTCCTTCTGAGCTCCCGAACCCGTGTTAACCCGTTGAGCCCTCCGATTCCACGTAAAATTGTCTACTGTGTAGCCAACACAGTCGACGTCGCTGCTGCTGGTGCCACCACCACCAAACCCAAGCATGCCGATGGCCAACTAGTCATTAAACGCGAGTTTGTCTCTCCTCCACTCACATTGGGCAAAGCCTCAACCTATCATTATGTTCTTAATGACCCAAGTTTACGATCGACTTGGGGCCATCGTGCGTGGGTGGCGAGTGGGTGCACCACAGTGCTCATTTCTTTGGCAAAATCAATAGTAGGTGCAGCCAGTTCACACATCTGGCTTGAGCCTACTTTAGCAGCATTGGTAGGTTACATTCTAGCAGACCTTGGATCTGGAGTTTACCATTGGGGCATTGATAACTATGGCGATGCCTCGACTCCAATCTTCGGTACCCAGATAGAAGCATTCCAAGGCCACCATAAGTGGCCATGGACAATCACGAAGCGTCAGTTTGCCAACAACTTGTATGCATTGGCACGAGCTGTGACATTCGTTGTGCTTCCCATAAACCTTGCATTTGGTGATCCCATTCTACATGGTTTTGTTAGTGTATGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCATGATAAAAAGAGCCAACTACCACCACTCGTGGTAGCAATGCAAGATGCTGGCCTGCTCGTGTCACGAATGCAACATTCCGCACATCATCGTCCACCCTATAACAACAACTATTGTATTGTAAGTGGAATATGGAATCAGCTTTTGGATGAAACAAAGGTTTTCGAGGCATTTGAGATGATCTTGTTTTTCAAGCTCGGGGTAAGACCAAGATCTTGGAGTGAGCCTAACACTGAATGGACCGAAGAGACTGAAACTGTAGAACAAACTACAGCC

Coding sequence (CDS)

ATGTCAATCTTAGCTCAGAACAAGTTCCTTCTGAGCTCCCGAACCCGTGTTAACCCGTTGAGCCCTCCGATTCCACGTAAAATTGTCTACTGTGTAGCCAACACAGTCGACGTCGCTGCTGCTGGTGCCACCACCACCAAACCCAAGCATGCCGATGGCCAACTAGTCATTAAACGCGAGTTTGTCTCTCCTCCACTCACATTGGGCAAAGCCTCAACCTATCATTATGTTCTTAATGACCCAAGTTTACGATCGACTTGGGGCCATCGTGCGTGGGTGGCGAGTGGGTGCACCACAGTGCTCATTTCTTTGGCAAAATCAATAGTAGGTGCAGCCAGTTCACACATCTGGCTTGAGCCTACTTTAGCAGCATTGGTAGGTTACATTCTAGCAGACCTTGGATCTGGAGTTTACCATTGGGGCATTGATAACTATGGCGATGCCTCGACTCCAATCTTCGGTACCCAGATAGAAGCATTCCAAGGCCACCATAAGTGGCCATGGACAATCACGAAGCGTCAGTTTGCCAACAACTTGTATGCATTGGCACGAGCTGTGACATTCGTTGTGCTTCCCATAAACCTTGCATTTGGTGATCCCATTCTACATGGTTTTGTTAGTGTATGCTCAGGTTGCATAATGTTTAGCCAACAGTTTCATGCATGGGCTCATGATAAAAAGAGCCAACTACCACCACTCGTGGTAGCAATGCAAGATGCTGGCCTGCTCGTGTCACGAATGCAACATTCCGCACATCATCGTCCACCCTATAACAACAACTATTGTATTGTAAGTGGAATATGGAATCAGCTTTTGGATGAAACAAAGGTTTTCGAGGCATTTGAGATGATCTTGTTTTTCAAGCTCGGGGTAAGACCAAGATCTTGGAGTGAGCCTAACACTGAATGGACCGAAGAGACTGAAACTGTAGAACAAACTACAGCC

Protein sequence

MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKREFVSPPLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLEPTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEPNTEWTEETETVEQTTA

Homology

[Close]

BLAST of MC10g1040.1 vs. ExPASy Swiss-Prot
Match: Q9SZ42 (Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 PE=1 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 3.1e-109
Identity = 178/264 (67.42%), Postives = 213/264 (80.68%), Query Frame = 0


Query: 45  TTKPKHADGQLVIKREFVSPPLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISL 104
           +TKP+    +LV+++   SPPL+           NDP+L+STW HR WVA+GCTT+ +SL
Sbjct: 65  STKPRPNREKLVVEQRLASPPLS-----------NDPTLKSTWTHRLWVAAGCTTLFVSL 124

Query: 105 AKSIVGAASSHIWLEPTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHH 164
           AKS++G   SH+ LEP LA   GYILADLGSGVYHW IDNYGD STP+ GTQIEAFQGHH
Sbjct: 125 AKSVIGGFDSHLCLEPALAGYAGYILADLGSGVYHWAIDNYGDESTPVVGTQIEAFQGHH 184

Query: 165 KWPWTITKRQFANNLYALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAH 224
           KWPWTIT+RQFANNL+ALA+ +TF VLP++LAF DP+ HGFV   + CI+FSQQFHAWAH
Sbjct: 185 KWPWTITRRQFANNLHALAQVITFTVLPLDLAFNDPVFHGFVCTFAFCILFSQQFHAWAH 244

Query: 225 DKKSQLPPLVVAMQDAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMI 284
             KS+LPPLVVA+QD GLLVSR QH+ HHR PYNNNYCIVSG WN +LDE+KVFEA EM+
Sbjct: 245 GTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPYNNNYCIVSGAWNNVLDESKVFEALEMV 304

Query: 285 LFFKLGVRPRSWSEPNTEWTEETE 309
            +F+LGVRPRSWSEPN++W EETE
Sbjct: 305 FYFQLGVRPRSWSEPNSDWIEETE 317

[Close]

BLAST of MC10g1040.1 vs. ExPASy Swiss-Prot
Match: O04584 (Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L1 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.4e-101
Identity = 173/294 (58.84%), Postives = 212/294 (72.11%), Query Frame = 0


Query: 18  NPLSP--PIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKREFVSPPLTLGKASTYH 77
           NPL P   IPR        T        TTT P+     LV+++  V+PPL+        
Sbjct: 9   NPLRPITNIPRS----YGPTRVRVTCSVTTTNPQLNHENLVVEKRLVNPPLSKN------ 68

Query: 78  YVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLEPTLAALVGYILADLGS 137
              NDP+L+STW HR WVA+G TT+  S AKSI+G   SH+WL+P LA   GY+ ADLGS
Sbjct: 69  ---NDPTLQSTWTHRLWVAAGSTTIFASFAKSIIGGFGSHLWLQPALACYAGYVFADLGS 128

Query: 138 GVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFVVLPINL 197
           GVYHW IDNYG ASTPI G Q+EA QGHHK+PWTITKRQFANN Y +ARA+TF+VLP+NL
Sbjct: 129 GVYHWAIDNYGGASTPIVGAQLEASQGHHKYPWTITKRQFANNSYTIARAITFIVLPLNL 188

Query: 198 AFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDAGLLVSRMQHSAHHRP 257
           A  +P+ H FVS  + CI+ SQQFHAWAH  KS+LPPLV+A+QD GLLVSR  H  HH+ 
Sbjct: 189 AINNPLFHSFVSTFAFCILLSQQFHAWAHGTKSKLPPLVMALQDMGLLVSRKDHPGHHQA 248

Query: 258 PYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEPNTEWTEETET 310
           PYN+NYC+VSG WN++LDE+ +F+A EM LFF+ GVRP SW+EPN++WTEETET
Sbjct: 249 PYNSNYCVVSGAWNKVLDESNLFKALEMALFFQFGVRPNSWNEPNSDWTEETET 289

[Close]

BLAST of MC10g1040.1 vs. ExPASy Swiss-Prot
Match: O81006 (Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L2 PE=2 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.6e-97
Identity = 165/292 (56.51%), Postives = 213/292 (72.95%), Query Frame = 0


Query: 11  LSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKREFVSPPLTLGK 70
           L ++  +NP++  IPR           +     T ++P H + +LV+++  V+PPL+   
Sbjct: 5   LQTKYTLNPITNNIPRS-----HRPSFLRVTSTTNSQPNH-EMKLVVEQRLVNPPLS--- 64

Query: 71  ASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLEPTLAALVGYIL 130
                   NDP+L+STW HR WVA+GCTTV +S +KSI+GA  SH+WLEP+LA   GYIL
Sbjct: 65  --------NDPTLQSTWTHRLWVAAGCTTVFVSFSKSIIGAFGSHLWLEPSLAGFAGYIL 124

Query: 131 ADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFVV 190
           ADLGSGVYHW  DNYGD STP+ G  IE  Q HHK PWTITKRQFANNL+ +AR  T +V
Sbjct: 125 ADLGSGVYHWATDNYGDESTPLVGIHIEDSQDHHKCPWTITKRQFANNLHFMARGTTLIV 184

Query: 191 LPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDAGLLVSRMQHS 250
           LP++LAF D ++HGFVS+ + C++F Q FHAWAH  KS+LPPLVV +QD GLLVSR+ H 
Sbjct: 185 LPLDLAFDDHVVHGFVSMFAFCVLFCQLFHAWAHGTKSKLPPLVVGLQDIGLLVSRIHHM 244

Query: 251 AHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEPNTE 303
            HHR PYNNNYC+VSG+WN++LDE+ VF+A EM+L+ +LGVRPRSW+EPN E
Sbjct: 245 NHHRAPYNNNYCVVSGVWNKVLDESNVFKAMEMVLYIQLGVRPRSWTEPNYE 279

[Close]

BLAST of MC10g1040.1 vs. ExPASy Swiss-Prot
Match: Q9AE87 (Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 3.6e-17
Identity = 61/199 (30.65%), Postives = 89/199 (44.72%), Query Frame = 0


Query: 102 ISLAKSIVGAASSHIWLEPTLAALVGYILADLGSGVYHWGIDNYGDASTPIFG-TQIEAF 161
           ++L   + G   +  WL  + A L+GY+ AD  SG  HW  D +G    P+ G   I  F
Sbjct: 42  VALVYRLWGTPYAGTWLLLS-AVLLGYLAADFVSGFVHWMGDTWGSTEMPVLGKALIRPF 101

Query: 162 QGHHKWPWTITKRQFA-----NNLYALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMF 221
           + HH     IT+  F      N L +L  A+  + LP++   G      F+      +M 
Sbjct: 102 REHHVDEKAITRHDFVETNGNNCLISLPVAIIALCLPMS-GPGWVFCASFLGAMIFWVMA 161

Query: 222 SQQFHAWAHDKKSQLPPLVVAMQDAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDET 281
           + QFH W+H      P LV  +Q   L++    H  HH  PYN  YCI  G  N+ L   
Sbjct: 162 TNQFHKWSH--MDSPPALVGFLQRVHLILPPDHHRIHHTKPYNKYYCITVGWMNKPLTMV 221

Query: 282 KVFEAFEMILFFKLGVRPR 295
             F   E ++ +  G+ PR
Sbjct: 222 HFFPTAERLITWATGLLPR 236

[Close]

BLAST of MC10g1040.1 vs. ExPASy Swiss-Prot
Match: Q5UR78 (Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L630 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 3.5e-12
Identity = 47/179 (26.26%), Postives = 80/179 (44.69%), Query Frame = 0


Query: 118 LEPTLAALVGYILADLGSGVYHWGIDNYGDASTPIFG-TQIEAFQGHHKWPWTITKRQFA 177
           ++  +  ++GY+L+D   G+YHW  D Y    TPI G T I   + HH  P  + +    
Sbjct: 6   IDKFIQIVLGYLLSDFIMGIYHWIKDTYFSPFTPIIGKTFIWGSRLHHVRPRYVLEFTDK 65

Query: 178 NNLYALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVA 237
           + +   A+     + P+   FG   L  F+      I  +   H + H+   + P     
Sbjct: 66  DLIIDSAKWTLSWIGPLFFWFG---LTPFLVTMFIMISLNDVIHKYTHEIDHERPMWATI 125

Query: 238 MQDAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRS 296
           +Q  G   S  +H  HH  P+  NYC V+   N  L++  ++   E  + +  GV+PR+
Sbjct: 126 LQRIGFFQSHDEHHLHHIAPHEINYCPVTPYVNIWLEKINLWRKLESFVEYLTGVKPRA 181

[Close]

BLAST of MC10g1040.1 vs. NCBI nr
Match: XP_022150986.1 (fatty acid desaturase 4, chloroplastic [Momordica charantia])

HSP 1 Score: 649 bits (1675), Expect = 2.21e-235
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60
           MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60

Query: 61  FVSPPLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLEP 120
           FVSPPLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLEP
Sbjct: 61  FVSPPLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLEP 120

Query: 121 TLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLY 180
           TLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLY
Sbjct: 121 TLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLY 180

Query: 181 ALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDA 240
           ALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDA
Sbjct: 181 ALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDA 240

Query: 241 GLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEPN 300
           GLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEPN
Sbjct: 241 GLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEPN 300

Query: 301 TEWTEETETVEQTTA 315
           TEWTEETETVEQTTA
Sbjct: 301 TEWTEETETVEQTTA 315

[Close]

BLAST of MC10g1040.1 vs. NCBI nr
Match: XP_038905900.1 (fatty acid desaturase 4, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 566 bits (1459), Expect = 1.93e-202
Identity = 270/316 (85.44%), Postives = 291/316 (92.09%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60
           MSILAQNKFLLSSR+ VNP S PIPR++ YC+AN+    AAGATTT PKHADGQL+I+RE
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVFYCIANSTVNVAAGATTTNPKHADGQLLIERE 60

Query: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120
            +SP PLTL  +STY+ VLNDPSLRSTW HRAWVASGCTTVL+SLAKSIVGAASSHIWLE
Sbjct: 61  VISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWLE 120

Query: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSGVYHWGIDNYG+ STPIFGTQIEAFQGHHKWPWTITKRQFANN+
Sbjct: 121 PALAALVGYILADLGSGVYHWGIDNYGNVSTPIFGTQIEAFQGHHKWPWTITKRQFANNM 180

Query: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240
           YALARAVTF VLP++LAF DPILHGFV VCSGCIMFSQQFHAWAH KKS+LPPLVVAMQD
Sbjct: 181 YALARAVTFAVLPVSLAFDDPILHGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQD 240

Query: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300
           AGLLVSR QHSAHHRPPYNNNYCIVSGIWN++LDETKVFEA EMILFFKLGVRPRSW+EP
Sbjct: 241 AGLLVSRRQHSAHHRPPYNNNYCIVSGIWNKILDETKVFEALEMILFFKLGVRPRSWTEP 300

Query: 301 NTEWTEETETVEQTTA 315
           N+ WTEETE +EQTTA
Sbjct: 301 NSAWTEETEIIEQTTA 316

[Close]

BLAST of MC10g1040.1 vs. NCBI nr
Match: XP_004152938.1 (fatty acid desaturase 4, chloroplastic [Cucumis sativus] >KGN56137.1 hypothetical protein Csa_011564 [Cucumis sativus])

HSP 1 Score: 550 bits (1418), Expect = 2.94e-196
Identity = 264/316 (83.54%), Postives = 284/316 (89.87%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60
           MSILAQNKFLLSSR+ VNP S PIPR++VYC+ANT          T PKHADGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTT----VNGVATSPKHADGQLVIERE 60

Query: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASSHIWLE
Sbjct: 61  VISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLE 120

Query: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240
           YALARAVTF VLP++LA  DPILHGFV +CSGCIMFSQQFHAWAH KKS+LPPLVVAMQ+
Sbjct: 181 YALARAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQN 240

Query: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300
           AGLLVSR QHS HHRPPYNNNYCIVSGIWN+LLDETK+FEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEP 300

Query: 301 NTEWTEETETVEQTTA 315
           N+EWTEETE +EQTTA
Sbjct: 301 NSEWTEETEILEQTTA 312

[Close]

BLAST of MC10g1040.1 vs. NCBI nr
Match: KAA0033959.1 (fatty acid desaturase 4 [Cucumis melo var. makuwa] >TYK02585.1 fatty acid desaturase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 541 bits (1394), Expect = 1.33e-192
Identity = 261/316 (82.59%), Postives = 279/316 (88.29%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60
           MSILAQNKFLLSSR+ VNP S P PR++VYC+ANT          T  KH DGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTT----VNGVATSSKHGDGQLVIERE 60

Query: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASS IWLE
Sbjct: 61  VISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLE 120

Query: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSGVYHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PALAALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240
           YALARAVTF VLP++LAF DP LHGFV VCSGCIMFSQQFHAWAH KKS++PPLVVAMQD
Sbjct: 181 YALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQD 240

Query: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300
           AGLLVSR QH+ HHRPPYNNNYCIVSGIWNQLLDETKVFEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEP 300

Query: 301 NTEWTEETETVEQTTA 315
           N+EWTEETE +EQTTA
Sbjct: 301 NSEWTEETEILEQTTA 312

[Close]

BLAST of MC10g1040.1 vs. NCBI nr
Match: XP_008463431.1 (PREDICTED: fatty acid desaturase 4, chloroplastic [Cucumis melo])

HSP 1 Score: 540 bits (1391), Expect = 3.81e-192
Identity = 260/316 (82.28%), Postives = 279/316 (88.29%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60
           MSILAQNKFLLSSR+ VNP S P PR++VYC+ANT          T  KH DGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTT----VNGVGTSSKHGDGQLVIERE 60

Query: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASS IWLE
Sbjct: 61  VISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLE 120

Query: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSG+YHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PALAALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240
           YALARAVTF VLP++LAF DP LHGFV VCSGCIMFSQQFHAWAH KKS++PPLVVAMQD
Sbjct: 181 YALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQD 240

Query: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300
           AGLLVSR QH+ HHRPPYNNNYCIVSGIWNQLLDETKVFEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEP 300

Query: 301 NTEWTEETETVEQTTA 315
           N+EWTEETE +EQTTA
Sbjct: 301 NSEWTEETEILEQTTA 312

[Close]

BLAST of MC10g1040.1 vs. ExPASy TrEMBL
Match: A0A6J1DD70 (fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019014 PE=3 SV=1)

HSP 1 Score: 649 bits (1675), Expect = 1.07e-235
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60
           MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60

Query: 61  FVSPPLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLEP 120
           FVSPPLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLEP
Sbjct: 61  FVSPPLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLEP 120

Query: 121 TLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLY 180
           TLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLY
Sbjct: 121 TLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLY 180

Query: 181 ALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDA 240
           ALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDA
Sbjct: 181 ALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDA 240

Query: 241 GLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEPN 300
           GLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEPN
Sbjct: 241 GLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEPN 300

Query: 301 TEWTEETETVEQTTA 315
           TEWTEETETVEQTTA
Sbjct: 301 TEWTEETETVEQTTA 315

[Close]

BLAST of MC10g1040.1 vs. ExPASy TrEMBL
Match: A0A0A0L6B9 (TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G077710 PE=3 SV=1)

HSP 1 Score: 550 bits (1418), Expect = 1.42e-196
Identity = 264/316 (83.54%), Postives = 284/316 (89.87%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60
           MSILAQNKFLLSSR+ VNP S PIPR++VYC+ANT          T PKHADGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTT----VNGVATSPKHADGQLVIERE 60

Query: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASSHIWLE
Sbjct: 61  VISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWLE 120

Query: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240
           YALARAVTF VLP++LA  DPILHGFV +CSGCIMFSQQFHAWAH KKS+LPPLVVAMQ+
Sbjct: 181 YALARAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQN 240

Query: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300
           AGLLVSR QHS HHRPPYNNNYCIVSGIWN+LLDETK+FEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTEP 300

Query: 301 NTEWTEETETVEQTTA 315
           N+EWTEETE +EQTTA
Sbjct: 301 NSEWTEETEILEQTTA 312

[Close]

BLAST of MC10g1040.1 vs. ExPASy TrEMBL
Match: A0A5A7SXQ7 (Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00380 PE=3 SV=1)

HSP 1 Score: 541 bits (1394), Expect = 6.45e-193
Identity = 261/316 (82.59%), Postives = 279/316 (88.29%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60
           MSILAQNKFLLSSR+ VNP S P PR++VYC+ANT          T  KH DGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTT----VNGVATSSKHGDGQLVIERE 60

Query: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASS IWLE
Sbjct: 61  VISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLE 120

Query: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSGVYHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PALAALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240
           YALARAVTF VLP++LAF DP LHGFV VCSGCIMFSQQFHAWAH KKS++PPLVVAMQD
Sbjct: 181 YALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQD 240

Query: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300
           AGLLVSR QH+ HHRPPYNNNYCIVSGIWNQLLDETKVFEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEP 300

Query: 301 NTEWTEETETVEQTTA 315
           N+EWTEETE +EQTTA
Sbjct: 301 NSEWTEETEILEQTTA 312

[Close]

BLAST of MC10g1040.1 vs. ExPASy TrEMBL
Match: A0A1S3CJN9 (fatty acid desaturase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501595 PE=3 SV=1)

HSP 1 Score: 540 bits (1391), Expect = 1.85e-192
Identity = 260/316 (82.28%), Postives = 279/316 (88.29%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKRE 60
           MSILAQNKFLLSSR+ VNP S P PR++VYC+ANT          T  KH DGQLVI+RE
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTT----VNGVGTSSKHGDGQLVIERE 60

Query: 61  FVSP-PLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLE 120
            +SP PLT   +STY+ VLNDPSLRSTW HRAWVA GCTTVLISLAKSIVGAASS IWLE
Sbjct: 61  VISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWLE 120

Query: 121 PTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180
           P LAALVGYILADLGSG+YHWGIDNYG ASTPIFGTQIEAFQGHHKWPWTITKRQFANNL
Sbjct: 121 PALAALVGYILADLGSGIYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANNL 180

Query: 181 YALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQD 240
           YALARAVTF VLP++LAF DP LHGFV VCSGCIMFSQQFHAWAH KKS++PPLVVAMQD
Sbjct: 181 YALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQD 240

Query: 241 AGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEP 300
           AGLLVSR QH+ HHRPPYNNNYCIVSGIWNQLLDETKVFEA EMIL+FKLG+RPRSW+EP
Sbjct: 241 AGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTEP 300

Query: 301 NTEWTEETETVEQTTA 315
           N+EWTEETE +EQTTA
Sbjct: 301 NSEWTEETEILEQTTA 312

[Close]

BLAST of MC10g1040.1 vs. ExPASy TrEMBL
Match: A0A6J1IGF1 (fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111476701 PE=3 SV=1)

HSP 1 Score: 537 bits (1383), Expect = 3.67e-191
Identity = 264/317 (83.28%), Postives = 281/317 (88.64%), Query Frame = 0


Query: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVANT-VDVAAAGATTTKPKHADGQLVIKR 60
           MSIL QNKFLLSS+T V+  SP +PRK V C+ANT V+VAAA     KPKH DGQLVI+R
Sbjct: 1   MSILVQNKFLLSSKTHVSLWSPAVPRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60

Query: 61  EFVSPPL-TLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWL 120
           E +SP    L  +STY+ VLNDPSL+STW HR WV SGCT VLISLAKSI GAASSH WL
Sbjct: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASSHTWL 120

Query: 121 EPTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180
           EP LAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHK PWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180

Query: 181 LYALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240
           LYALARAVTF VLPI LA  DPILHGFV+VCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ
Sbjct: 181 LYALARAVTFTVLPITLAVNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSE 300
           DAGLLVSR+QHSAHHRPPYNNNYCIVSGIWN+LLDETKVFEA EMILFFKLGVRPRSW+E
Sbjct: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PNTEWTEETETVEQTTA 315
           P++EWTEETE +EQTTA
Sbjct: 301 PHSEWTEETEIIEQTTA 317

[Close]

BLAST of MC10g1040.1 vs. TAIR 10
Match: AT4G27030.1 (fatty acid desaturase A )

HSP 1 Score: 396.4 bits (1017), Expect = 2.2e-110
Identity = 178/264 (67.42%), Postives = 213/264 (80.68%), Query Frame = 0


Query: 45  TTKPKHADGQLVIKREFVSPPLTLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISL 104
           +TKP+    +LV+++   SPPL+           NDP+L+STW HR WVA+GCTT+ +SL
Sbjct: 65  STKPRPNREKLVVEQRLASPPLS-----------NDPTLKSTWTHRLWVAAGCTTLFVSL 124

Query: 105 AKSIVGAASSHIWLEPTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHH 164
           AKS++G   SH+ LEP LA   GYILADLGSGVYHW IDNYGD STP+ GTQIEAFQGHH
Sbjct: 125 AKSVIGGFDSHLCLEPALAGYAGYILADLGSGVYHWAIDNYGDESTPVVGTQIEAFQGHH 184

Query: 165 KWPWTITKRQFANNLYALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAH 224
           KWPWTIT+RQFANNL+ALA+ +TF VLP++LAF DP+ HGFV   + CI+FSQQFHAWAH
Sbjct: 185 KWPWTITRRQFANNLHALAQVITFTVLPLDLAFNDPVFHGFVCTFAFCILFSQQFHAWAH 244

Query: 225 DKKSQLPPLVVAMQDAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMI 284
             KS+LPPLVVA+QD GLLVSR QH+ HHR PYNNNYCIVSG WN +LDE+KVFEA EM+
Sbjct: 245 GTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPYNNNYCIVSGAWNNVLDESKVFEALEMV 304

Query: 285 LFFKLGVRPRSWSEPNTEWTEETE 309
            +F+LGVRPRSWSEPN++W EETE
Sbjct: 305 FYFQLGVRPRSWSEPNSDWIEETE 317

[Close]

BLAST of MC10g1040.1 vs. TAIR 10
Match: AT1G62190.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 370.2 bits (949), Expect = 1.7e-102
Identity = 173/294 (58.84%), Postives = 212/294 (72.11%), Query Frame = 0


Query: 18  NPLSP--PIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKREFVSPPLTLGKASTYH 77
           NPL P   IPR        T        TTT P+     LV+++  V+PPL+        
Sbjct: 9   NPLRPITNIPRS----YGPTRVRVTCSVTTTNPQLNHENLVVEKRLVNPPLSKN------ 68

Query: 78  YVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLEPTLAALVGYILADLGS 137
              NDP+L+STW HR WVA+G TT+  S AKSI+G   SH+WL+P LA   GY+ ADLGS
Sbjct: 69  ---NDPTLQSTWTHRLWVAAGSTTIFASFAKSIIGGFGSHLWLQPALACYAGYVFADLGS 128

Query: 138 GVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFVVLPINL 197
           GVYHW IDNYG ASTPI G Q+EA QGHHK+PWTITKRQFANN Y +ARA+TF+VLP+NL
Sbjct: 129 GVYHWAIDNYGGASTPIVGAQLEASQGHHKYPWTITKRQFANNSYTIARAITFIVLPLNL 188

Query: 198 AFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDAGLLVSRMQHSAHHRP 257
           A  +P+ H FVS  + CI+ SQQFHAWAH  KS+LPPLV+A+QD GLLVSR  H  HH+ 
Sbjct: 189 AINNPLFHSFVSTFAFCILLSQQFHAWAHGTKSKLPPLVMALQDMGLLVSRKDHPGHHQA 248

Query: 258 PYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEPNTEWTEETET 310
           PYN+NYC+VSG WN++LDE+ +F+A EM LFF+ GVRP SW+EPN++WTEETET
Sbjct: 249 PYNSNYCVVSGAWNKVLDESNLFKALEMALFFQFGVRPNSWNEPNSDWTEETET 289

[Close]

BLAST of MC10g1040.1 vs. TAIR 10
Match: AT2G22890.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 357.5 bits (916), Expect = 1.1e-98
Identity = 165/292 (56.51%), Postives = 213/292 (72.95%), Query Frame = 0


Query: 11  LSSRTRVNPLSPPIPRKIVYCVANTVDVAAAGATTTKPKHADGQLVIKREFVSPPLTLGK 70
           L ++  +NP++  IPR           +     T ++P H + +LV+++  V+PPL+   
Sbjct: 5   LQTKYTLNPITNNIPRS-----HRPSFLRVTSTTNSQPNH-EMKLVVEQRLVNPPLS--- 64

Query: 71  ASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWLEPTLAALVGYIL 130
                   NDP+L+STW HR WVA+GCTTV +S +KSI+GA  SH+WLEP+LA   GYIL
Sbjct: 65  --------NDPTLQSTWTHRLWVAAGCTTVFVSFSKSIIGAFGSHLWLEPSLAGFAGYIL 124

Query: 131 ADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANNLYALARAVTFVV 190
           ADLGSGVYHW  DNYGD STP+ G  IE  Q HHK PWTITKRQFANNL+ +AR  T +V
Sbjct: 125 ADLGSGVYHWATDNYGDESTPLVGIHIEDSQDHHKCPWTITKRQFANNLHFMARGTTLIV 184

Query: 191 LPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDAGLLVSRMQHS 250
           LP++LAF D ++HGFVS+ + C++F Q FHAWAH  KS+LPPLVV +QD GLLVSR+ H 
Sbjct: 185 LPLDLAFDDHVVHGFVSMFAFCVLFCQLFHAWAHGTKSKLPPLVVGLQDIGLLVSRIHHM 244

Query: 251 AHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSEPNTE 303
            HHR PYNNNYC+VSG+WN++LDE+ VF+A EM+L+ +LGVRPRSW+EPN E
Sbjct: 245 NHHRAPYNNNYCVVSGVWNKVLDESNVFKAMEMVLYIQLGVRPRSWTEPNYE 279

The following BLAST results are available for this feature:

BLAST of MC10g1040.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZ42	3.1e-109	67.42	Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 P...	[more]
O04584	2.4e-101	58.84	Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
O81006	1.6e-97	56.51	Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9AE87	3.6e-17	30.65	Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1	[more]
Q5UR78	3.5e-12	26.26	Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=...	[more]

BLAST of MC10g1040.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150986.1	2.21e-235	100.00	fatty acid desaturase 4, chloroplastic [Momordica charantia]	[more]
XP_038905900.1	1.93e-202	85.44	fatty acid desaturase 4, chloroplastic-like [Benincasa hispida]	[more]
XP_004152938.1	2.94e-196	83.54	fatty acid desaturase 4, chloroplastic [Cucumis sativus] >KGN56137.1 hypothetica...	[more]
KAA0033959.1	1.33e-192	82.59	fatty acid desaturase 4 [Cucumis melo var. makuwa] >TYK02585.1 fatty acid desatu...	[more]
XP_008463431.1	3.81e-192	82.28	PREDICTED: fatty acid desaturase 4, chloroplastic [Cucumis melo]	[more]

BLAST of MC10g1040.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DD70	1.07e-235	100.00	fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0L6B9	1.42e-196	83.54	TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G07...	[more]
A0A5A7SXQ7	6.45e-193	82.59	Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3CJN9	1.85e-192	82.28	fatty acid desaturase 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501595 P...	[more]
A0A6J1IGF1	3.67e-191	83.28	fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114767...	[more]

BLAST of MC10g1040.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27030.1	2.2e-110	67.42	fatty acid desaturase A	[more]
AT1G62190.1	1.7e-102	58.84	Kua-ubiquitin conjugating enzyme hybrid localisation domain	[more]
AT2G22890.1	1.1e-98	56.51	Kua-ubiquitin conjugating enzyme hybrid localisation domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019547	B domain of TMEM189, localisation domain	PFAM	PF10520	TMEM189_B_dmain	coord: 128..296 e-value: 4.9E-64 score: 215.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 297..315
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 295..315
None	No IPR available	PANTHER	PTHR48140	FAMILY NOT NAMED	coord: 1..312

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC10g1040	MC10g1040	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC10g1040.1-cds	MC10g1040.1-cds-MC10:10202765..10203709	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC10g1040.1	MC10g1040.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006631	fatty acid metabolic process
cellular_component	GO:0016021	integral component of membrane