MC09g0385.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC09g0385.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	NAD-specific glutamate dehydrogenase
Location	MC09: 3513512 .. 3513709 (+)
Sequence length	198
RNA-Seq Expression	MC09g0385.1
Synteny	MC09g0385.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGATTCATCGCGACCTGATTCTTGGCCGCATCGCCGATCAGTCGTTCGGTATCGGTGAAAGCGACGTAGGAGGGAGTGGTTCTGTTGCCTTGATCATTGGCAATAATCTCTACACGATCGTTTTGCCAGACGGCGACGCAGCTGTAGGTGGTGCCGAGATCAATGCCGATGCCTTTGCCTTCGCTCTTGCAAGTCAT

mRNA sequence

CGGATTCATCGCGACCTGATTCTTGGCCGCATCGCCGATCAGTCGTTCGGTATCGGTGAAAGCGACGTAGGAGGGAGTGGTTCTGTTGCCTTGATCATTGGCAATAATCTCTACACGATCGTTTTGCCAGACGGCGACGCAGCTGTAGGTGGTGCCGAGATCAATGCCGATGCCTTTGCCTTCGCTCTTGCAAGTCAT

Coding sequence (CDS)

CGGATTCATCGCGACCTGATTCTTGGCCGCATCGCCGATCAGTCGTTCGGTATCGGTGAAAGCGACGTAGGAGGGAGTGGTTCTGTTGCCTTGATCATTGGCAATAATCTCTACACGATCGTTTTGCCAGACGGCGACGCAGCTGTAGGTGGTGCCGAGATCAATGCCGATGCCTTTGCCTTCGCTCTTGCAAGTCAT

Protein sequence

RIHRDLILGRIADQSFGIGESDVGGSGSVALIIGNNLYTIVLPDGDAAVGGAEINADAFAFALASH

Homology

[Close]

BLAST of MC09g0385.1 vs. ExPASy Swiss-Prot
Match: P41755 (NAD-specific glutamate dehydrogenase OS=Achlya klebsiana OX=4767 PE=3 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 5.5e-08
Identity = 26/57 (45.61%), Postives = 38/57 (66.67%), Query Frame = 0


Query: 1    RIHRDLILGRIADQSFGIGESDVGGSGSVALIIGNNLYTIVLPDGDAAVGGAEINAD 58
            R+H +L+L  I +++F + ES+V   G+V LI+GNN  TIVLPD D  +   EI+ D
Sbjct: 979  RVHGNLVLSGITNKTFAVSESNVRWGGTVTLIVGNNFNTIVLPDTDTRISRTEIDTD 1035

[Close]

BLAST of MC09g0385.1 vs. ExPASy Swiss-Prot
Match: P39703 (Putative uncharacterized protein YAL004W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YAL004W PE=5 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 3.9e-06
Identity = 24/41 (58.54%), Postives = 30/41 (73.17%), Query Frame = 0


Query: 1   RIHRDLILGRIADQSFGIGESDVGGSGSVALIIGNNLYTIV 42
           RIH  LIL  I +QSF + ESD   SGSV LI+GNN++TI+
Sbjct: 164 RIHSSLILSSITNQSFSVSESDKRWSGSVTLIVGNNVHTII 204

[Close]

BLAST of MC09g0385.1 vs. NCBI nr
Match: CAA7406321.1 (unnamed protein product [Spirodela intermedia])

HSP 1 Score: 75.5 bits (184), Expect = 6.72e-14
Identity = 33/66 (50.00%), Postives = 50/66 (75.76%), Query Frame = 0


Query: 1   RIHRDLILGRIADQSFGIGESDVGGSGSVALIIGNNLYTIVLPDGDAAVGGAEINADAFA 60
           R+H +L+LG +AD++  +GE+D+G  G+V L++G++L+TIVLPD DA VG  EINAD   
Sbjct: 554 RVHGNLVLGGVADEALAVGEADIGRRGAVTLVVGDDLHTIVLPDPDARVGRTEINADRRT 613

Query: 61  FALASH 66
           F+ + H
Sbjct: 614 FSFSGH 619

[Close]

BLAST of MC09g0385.1 vs. NCBI nr
Match: KAF1755604.1 (hypothetical protein GCK72_012054 [Caenorhabditis remanei])

HSP 1 Score: 65.1 bits (157), Expect = 6.31e-12
Identity = 31/56 (55.36%), Postives = 45/56 (80.36%), Query Frame = 0


Query: 2  IHRDLILGRIADQSFGIGESDVGGSGSVALIIGNNLYTIVLPDGDAAVGGAEINAD 57
          +H DLILG I++Q+ G+GESDV  SGSV+L++GN+L   VL D +A VGG+EI+++
Sbjct: 13 VHGDLILGGISNQTLGVGESDVRWSGSVSLVVGNDLDLSVLEDSNAGVGGSEIDSN 68

[Close]

BLAST of MC09g0385.1 vs. NCBI nr
Match: PNX99055.1 (hypothetical protein L195_g022316, partial [Trifolium pratense])

HSP 1 Score: 63.2 bits (152), Expect = 4.77e-11
Identity = 31/53 (58.49%), Postives = 42/53 (79.25%), Query Frame = 0


Query: 5  DLILGRIADQSFGIGESDVGGSGSVALIIGNNLYTIVLPDGDAAVGGAEINAD 57
          DLIL  +AD++ GI ESDV G G VALIIG+++ ++VLP  DA VGGAE+++D
Sbjct: 15 DLILCGVADEAVGISESDVRGGGPVALIIGDDMDSVVLPYSDAGVGGAEVDSD 67

[Close]

BLAST of MC09g0385.1 vs. NCBI nr
Match: KAE9531643.1 (hypothetical protein AGLY_010849 [Aphis glycines])

HSP 1 Score: 65.1 bits (157), Expect = 3.05e-10
Identity = 28/56 (50.00%), Postives = 40/56 (71.43%), Query Frame = 0


Query: 2   IHRDLILGRIADQSFGIGESDVGGSGSVALIIGNNLYTIVLPDGDAAVGGAEINAD 57
           +HR L+LGR+ADQ FG+GE  +   G V LI+G++LY  VLPD  A +GG +++ D
Sbjct: 425 VHRHLVLGRVADQPFGVGERHITRGGPVTLIVGDDLYFSVLPDTHARIGGTQVDTD 480

[Close]

BLAST of MC09g0385.1 vs. NCBI nr
Match: MCI90087.1 (hypothetical protein [Trifolium medium])

HSP 1 Score: 59.7 bits (143), Expect = 5.22e-10
Identity = 26/47 (55.32%), Postives = 38/47 (80.85%), Query Frame = 0


Query: 11 IADQSFGIGESDVGGSGSVALIIGNNLYTIVLPDGDAAVGGAEINAD 57
          + D++ GI ESDV G G VAL++G++L ++VLPD DA VGGAE+++D
Sbjct: 1  VTDEAVGISESDVRGGGPVALVVGDDLDSVVLPDSDAGVGGAEVDSD 47

[Close]

BLAST of MC09g0385.1 vs. ExPASy TrEMBL
Match: A0A453FWD4 (Uncharacterized protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.82e-15
Identity = 31/56 (55.36%), Postives = 47/56 (83.93%), Query Frame = 0


Query: 2  IHRDLILGRIADQSFGIGESDVGGSGSVALIIGNNLYTIVLPDGDAAVGGAEINAD 57
          +H DL+LG +AD++ G+GE+DVGG G+VAL+I ++L+ +VLP  DA VGGA+++AD
Sbjct: 14 VHGDLVLGGVADEALGVGEADVGGRGAVALVIRDDLHPVVLPHADARVGGAQVDAD 69

[Close]

BLAST of MC09g0385.1 vs. ExPASy TrEMBL
Match: A0A7I8L8F4 (Hypothetical protein OS=Spirodela intermedia OX=51605 GN=SI8410_12016999 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 3.25e-14
Identity = 33/66 (50.00%), Postives = 50/66 (75.76%), Query Frame = 0


Query: 1   RIHRDLILGRIADQSFGIGESDVGGSGSVALIIGNNLYTIVLPDGDAAVGGAEINADAFA 60
           R+H +L+LG +AD++  +GE+D+G  G+V L++G++L+TIVLPD DA VG  EINAD   
Sbjct: 554 RVHGNLVLGGVADEALAVGEADIGRRGAVTLVVGDDLHTIVLPDPDARVGRTEINADRRT 613

Query: 61  FALASH 66
           F+ + H
Sbjct: 614 FSFSGH 619

[Close]

BLAST of MC09g0385.1 vs. ExPASy TrEMBL
Match: A0A287G6D9 (Uncharacterized protein OS=Hordeum vulgare subsp. vulgare OX=112509 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 7.41e-13
Identity = 32/62 (51.61%), Postives = 47/62 (75.81%), Query Frame = 0


Query: 2   IHRDLILGRIADQSFGIGESDVGGSGSVALIIGNNLYTIVLPDGDAAVGGAEINADAFAF 61
           +H DL+LG +AD++ G+GE DVGG G+VAL++G++L  ++LP  DA VG AE++AD    
Sbjct: 623 VHGDLVLGGVADEALGVGEGDVGGRGAVALVVGDDLDPVMLPHADAGVGRAEVDADRRTL 682

Query: 62  AL 63
           AL
Sbjct: 683 AL 684

[Close]

BLAST of MC09g0385.1 vs. ExPASy TrEMBL
Match: A0A6A5GLP9 (Uncharacterized protein OS=Caenorhabditis remanei OX=31234 GN=GCK72_012054 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.05e-12
Identity = 31/56 (55.36%), Postives = 45/56 (80.36%), Query Frame = 0


Query: 2  IHRDLILGRIADQSFGIGESDVGGSGSVALIIGNNLYTIVLPDGDAAVGGAEINAD 57
          +H DLILG I++Q+ G+GESDV  SGSV+L++GN+L   VL D +A VGG+EI+++
Sbjct: 13 VHGDLILGGISNQTLGVGESDVRWSGSVSLVVGNDLDLSVLEDSNAGVGGSEIDSN 68

[Close]

BLAST of MC09g0385.1 vs. ExPASy TrEMBL
Match: A0A7N0UQF0 (Uncharacterized protein OS=Kalanchoe fedtschenkoi OX=63787 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.68e-11
Identity = 31/63 (49.21%), Postives = 47/63 (74.60%), Query Frame = 0


Query: 1   RIHRDLILGRIADQSFGIGESDVGGSGSVALIIGNNLYTIVLPDGDAAVGGAEINADAFA 60
           R+H DL+L R+ADQ+  +GE  VGG G+VAL++G++L+ +VLP   AAV  AEI+ D+ +
Sbjct: 623 RVHGDLVLRRVADQTLRVGEGHVGGRGAVALVVGDDLHPVVLPHAHAAVRRAEIDPDSLS 682

Query: 61  FAL 63
           F+ 
Sbjct: 683 FSF 685

The following BLAST results are available for this feature:

BLAST of MC09g0385.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P41755	5.5e-08	45.61	NAD-specific glutamate dehydrogenase OS=Achlya klebsiana OX=4767 PE=3 SV=1	[more]
P39703	3.9e-06	58.54	Putative uncharacterized protein YAL004W OS=Saccharomyces cerevisiae (strain ATC...	[more]

BLAST of MC09g0385.1 vs. NCBI nr


Match Name	E-value	Identity	Description
CAA7406321.1	6.72e-14	50.00	unnamed protein product [Spirodela intermedia]	[more]
KAF1755604.1	6.31e-12	55.36	hypothetical protein GCK72_012054 [Caenorhabditis remanei]	[more]
PNX99055.1	4.77e-11	58.49	hypothetical protein L195_g022316, partial [Trifolium pratense]	[more]
KAE9531643.1	3.05e-10	50.00	hypothetical protein AGLY_010849 [Aphis glycines]	[more]
MCI90087.1	5.22e-10	55.32	hypothetical protein [Trifolium medium]	[more]

BLAST of MC09g0385.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A453FWD4	3.82e-15	55.36	Uncharacterized protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=4 S...	[more]
A0A7I8L8F4	3.25e-14	50.00	Hypothetical protein OS=Spirodela intermedia OX=51605 GN=SI8410_12016999 PE=4 SV...	[more]
A0A287G6D9	7.41e-13	51.61	Uncharacterized protein OS=Hordeum vulgare subsp. vulgare OX=112509 PE=4 SV=1	[more]
A0A6A5GLP9	3.05e-12	55.36	Uncharacterized protein OS=Caenorhabditis remanei OX=31234 GN=GCK72_012054 PE=4 ...	[more]
A0A7N0UQF0	1.68e-11	49.21	Uncharacterized protein OS=Kalanchoe fedtschenkoi OX=63787 PE=4 SV=1	[more]

BLAST of MC09g0385.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019651	Glutamate dehydrogenase, NAD-specific	PFAM	PF10712	NAD-GH	coord: 1..60 e-value: 1.0E-8 score: 34.1

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC09g0385	MC09g0385	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC09g0385.1-cds	MC09g0385.1-cds-MC09:3513512..3513709	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC09g0385.1	MC09g0385.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0003824	catalytic activity