MC08g1405.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC08g1405.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	ribonuclease MC-like
Location	MC08: 15313756 .. 15314503 (-)
Sequence length	651
RNA-Seq Expression	MC08g1405.1
Synteny	MC08g1405.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAGAAAGGAATTGCGTTGGTTCTGAGCACCTTGGTAGTGATAGCAAGCTGTGATTACAATTACTACCAAGTTACACTGCAATGGCAACCAGCTACATGCAGTGATACAAACCTGCAGAGTCCATGTGTTAACACTCCACAAGATAGATTTAGTATCCATGGCGTGTGGACATGGCGTGTGGCCTACTTTATCGTCGGGATCTTATCCACAATGTCCTGGTACTCCGGCCTACGATGGTAGCAAGGTAACTCATATTTGTAACCCCATGTTGGTTTAATTAAATTATTTTCAAAATATAAGGATGCATAATATTTGATTCTCTCTAGATCTCCGCGATAAGAAATCGTCTAAACACCGATTGGCCAAGTGTAATCTACGGAACAAACCCGATACTATGGAGCCACGAGTGGACAACCCATGGAACATGCTCTTATCCATCTTTGAGTCAACTGGACTATTTTACGTTCGGACTAGACACGTATGCTAAATACGATATCTTATCCGCTCTCGATAAAGAAGACATCTATCCTAGCGAAGACCTATACACAAAAAAAGTATCAATGACGCAATTCGCAAGATAACACAGAAGTTGCCAGGCCTTCGGTGCAACACAAATGATTACTCCCGCGAGAGACAGTTGCATGAAATTGTTTTGTGTTTCGCAAAGAGCGATCGGGCCATTATAGACTGTCCGTCCAGTGTTGTCACGTGCGGCAATTACGTGTATTGGTACCCATGGTAT

mRNA sequence

ATGGAAAAGAAAGGAATTGCGTTGGTTCTGAGCACCTTGGTAGTGATAGCAAGCTGTGATTACAATTACTACCAAGTTACACTGCAATGGCAACCAGCTACATGCAGTGATACAAACCTGCAGAGTCCATGTGTTAACACTCCACAAGATAGATTTAGTATCCATGGCGTGTGGCCTACTTTATCGTCGGGATCTTATCCACAATGTCCTGGTACTCCGGCCTACGATGGTAGCAAGATCTCCGCGATAAGAAATCGTCTAAACACCGATTGGCCAAGTGTAATCTACGGAACAAACCCGATACTATGGAGCCACGAGTGGACAACCCATGGAACATGCTCTTATCCATCTTTGAGTCAACTGGACTATTTTACGTTCGGACTAGACACGTATGCTAAATACGATATCTTATCCGCTCTCGATAAAGAAGACATCTATCCTAGCGAAGACCTATACACAAAAAGTATCAATGACGCAATTCGCAAGATAACACAGAAGTTGCCAGGCCTTCGGTGCAACACAAATGATTACTCCCGCGAGAGACAGTTGCATGAAATTGTTTTGTGTTTCGCAAAGAGCGATCGGGCCATTATAGACTGTCCGTCCAGTGTTGTCACGTGCGGCAATTACGTGTATTGGTACCCATGGTAT

Coding sequence (CDS)

ATGGAAAAGAAAGGAATTGCGTTGGTTCTGAGCACCTTGGTAGTGATAGCAAGCTGTGATTACAATTACTACCAAGTTACACTGCAATGGCAACCAGCTACATGCAGTGATACAAACCTGCAGAGTCCATGTGTTAACACTCCACAAGATAGATTTAGTATCCATGGCGTGTGGCCTACTTTATCGTCGGGATCTTATCCACAATGTCCTGGTACTCCGGCCTACGATGGTAGCAAGATCTCCGCGATAAGAAATCGTCTAAACACCGATTGGCCAAGTGTAATCTACGGAACAAACCCGATACTATGGAGCCACGAGTGGACAACCCATGGAACATGCTCTTATCCATCTTTGAGTCAACTGGACTATTTTACGTTCGGACTAGACACGTATGCTAAATACGATATCTTATCCGCTCTCGATAAAGAAGACATCTATCCTAGCGAAGACCTATACACAAAAAGTATCAATGACGCAATTCGCAAGATAACACAGAAGTTGCCAGGCCTTCGGTGCAACACAAATGATTACTCCCGCGAGAGACAGTTGCATGAAATTGTTTTGTGTTTCGCAAAGAGCGATCGGGCCATTATAGACTGTCCGTCCAGTGTTGTCACGTGCGGCAATTACGTGTATTGGTACCCATGGTAT

Protein sequence

MEKKGIALVLSTLVVIASCDYNYYQVTLQWQPATCSDTNLQSPCVNTPQDRFSIHGVWPTLSSGSYPQCPGTPAYDGSKISAIRNRLNTDWPSVIYGTNPILWSHEWTTHGTCSYPSLSQLDYFTFGLDTYAKYDILSALDKEDIYPSEDLYTKSINDAIRKITQKLPGLRCNTNDYSRERQLHEIVLCFAKSDRAIIDCPSSVVTCGNYVYWYPWY

Homology

[Close]

BLAST of MC08g1405.1 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.6e-30
Identity = 70/186 (37.63%), Postives = 101/186 (54.30%), Query Frame = 0


Query: 29  QWQPATCSDTNLQSPCVNTPQDRFSIHGVWPTLSSGSYPQCPGTPAYDGSKISAIRNRLN 88
           QW PA CS     S C  +    F+IHG+WP  S  S   CP    +D +KIS ++++LN
Sbjct: 9   QWPPAVCSFQKSGS-CPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKISHLQSQLN 68

Query: 89  TDWPSVIYGTNPILWSHEWTTHGTCSYPSLSQLDYFTFGLDTYAKYDILSALDKEDIYPS 148
           T WP+V+   N   WSHEWT HGTCS  + +Q  YF   +D    YDI+ AL      P+
Sbjct: 69  TLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALRPHAAGPN 128

Query: 149 EDLYTK-SINDAIRKITQKLPGLRCNTNDYSRERQLHEIVLCFAKSDRAIIDCPSSVVTC 208
               ++ +I   ++    K PGLRC T+  ++   L ++V CFA+    +IDC     TC
Sbjct: 129 GRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDCTRD--TC 188

Query: 209 G-NYVY 213
           G N+++
Sbjct: 189 GANFIF 191

[Close]

BLAST of MC08g1405.1 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 119.8 bits (299), Expect = 3.9e-26
Identity = 70/213 (32.86%), Postives = 113/213 (53.05%), Query Frame = 0


Query: 6   IALVLSTLVVIASC-------------DYNYYQVTLQWQPATCSDTNLQSPCVNTPQDRF 65
           IA +LS L++ ++              D+++Y    QW  + C     Q+   N  ++ F
Sbjct: 4   IAALLSVLLIASTAQSTVTIYESSKPGDFDFYLFVQQWIYSYCDS---QTCIQNKEREAF 63

Query: 66  SIHGVWPTLSSGSYPQCPGTPAYDGSKISAIRNRLNTDWPSVIYGTNPILWSHEWTTHGT 125
           +IHG+WP  S GSYP     P+++ + I  + ++LN DWPS + G N   W+HE++ HGT
Sbjct: 64  TIHGLWPENSDGSYPSFCSGPSFNVNAIQDLEDQLNFDWPS-LTGPNTDFWTHEFSKHGT 123

Query: 126 CSY--PSLSQLDYFTFGLDTYAKYDILSALDKEDIYPSEDLYTK--SINDAIRKITQKLP 185
           CS   P     DYF  G+  Y +++I +AL+ E+IYPS+    K   I +AI       P
Sbjct: 124 CSITGPITDIHDYFATGIKLYTEFNITAALESENIYPSDSNTYKPVDITNAITTHFGGKP 183

Query: 186 GLRCNTNDYSRERQLHEIVLCFAKSDRAIIDCP 202
           G++C++       QL  + +C  K+  +I+DCP
Sbjct: 184 GIQCSSG------QLSTVAVCIDKNSLSIMDCP 206

[Close]

BLAST of MC08g1405.1 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 9.5e-25
Identity = 67/207 (32.37%), Postives = 103/207 (49.76%), Query Frame = 0


Query: 9   VLSTLVVIASC--------DYNYYQVTLQWQPATCSDTNLQS--PCVNTPQDRFSIHGVW 68
           ++S LV++ S         D++++    QW P +  DT  +   P    P   F IHG+W
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQW-PGSYCDTQKKCCYPNSGKPAADFGIHGLW 69

Query: 69  PTLSSGSYP-QCPGTPAYDGSKISAIRNRLNTDWPSVI--YGTNPILWSHEWTTHGTCSY 128
           P    G+YP  C  +  +D S IS +   +   WP++    G+    W HEW  HGTCS 
Sbjct: 70  PNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSE 129

Query: 129 PSLSQLDYFTFGLDTYAKYDILSALDKEDIYPSEDLYT-KSINDAIRKITQKLPGLRCNT 188
             + Q +YF   L+   K ++L AL K  I P    Y+ +SI D+I++     P + CN 
Sbjct: 130 SVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECN- 189

Query: 189 NDYSRERQLHEIVLCFAKSDRAIIDCP 202
            D S   QL+++ LC  +S   +I+CP
Sbjct: 190 RDGSGNSQLYQVYLCVDRSGSGLIECP 214

[Close]

BLAST of MC08g1405.1 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-23
Identity = 71/203 (34.98%), Postives = 102/203 (50.25%), Query Frame = 0


Query: 8   LVLSTLVV--IASCDYNYYQVTLQWQPATCSDTNLQS--PCVNTPQDRFSIHGVWPTLSS 67
           +V+  L+V  + S D++++    QW PA+  DT      P    P + FSIHG+WP    
Sbjct: 13  IVVQCLLVLCVTSQDFDFFYFVQQW-PASYCDTRRSCCYPTTGKPDEDFSIHGLWPNYKD 72

Query: 68  GSYPQ-CPGTPAYDGSKISAIRNRLNTDWPSVIYGTNPIL--WSHEWTTHGTCSYPSLSQ 127
           G +PQ C    + D S+ S + + +  +WPS+   ++  L  WSHEW  HGTCS  +L+Q
Sbjct: 73  GKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGTCS--ALNQ 132

Query: 128 LDYFTFGLDTYAKYDILSALDKEDIYPSEDLY--TKSINDAIRKITQKLPGLRCNTNDYS 187
             YF   LD   K ++L  L+   I P    Y   +SI  AI K     P + CN  D  
Sbjct: 133 HAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPFIECNV-DSQ 192

Query: 188 RERQLHEIVLCFAKSDRAIIDCP 202
              QL+++ LC   S    IDCP
Sbjct: 193 GNHQLYQVYLCVDSSASKFIDCP 211

[Close]

BLAST of MC08g1405.1 vs. ExPASy Swiss-Prot
Match: P83618 (Ribonuclease-like storage protein OS=Panax ginseng OX=4054 PE=1 SV=2)

HSP 1 Score: 100.1 bits (248), Expect = 3.2e-20
Identity = 67/197 (34.01%), Postives = 96/197 (48.73%), Query Frame = 0


Query: 17  ASCDYNY--YQVTLQWQPATCSDTNL--QSPCVNTPQDRFSIHGVWPTLSSGSYP-QCPG 76
           A  DY +  + + LQW    C   N       +NT    F+IHG++P  + G+    C G
Sbjct: 23  ARSDYPWAMFALRLQWPAGFCEVNNACDTKSLLNT----FTIHGLYPYNAKGTPALYCDG 82

Query: 77  TPAYDGSKISAIRNRLNTDWPSVIYGTNPI-LWSHEWTTHGTCSYPSLSQLDYFTFGLDT 136
           T A+D + +S     ++  WPS    T  I  W HEW  HG CS   L Q DYF   L  
Sbjct: 83  T-AFDVNSVSDFLAEMHLAWPSHETNTEDIQFWEHEWKKHGRCSEALLKQTDYFRTALAF 142

Query: 137 YAKYDILSALDKEDIYPSEDLY-TKSINDAIRKITQKLPGLRCNTNDYSRERQLHEIVLC 196
              +DI+  L++E IYP+ DLY  K I +AI+K    +P +    N+ S E  L +I +C
Sbjct: 143 RKAFDIVGLLNQEGIYPNNDLYRPKMIKEAIKKHLNAVPEIDFTKNENS-EYVLTDINVC 202

Query: 197 FAKSDRAIIDCPSSVVT 207
             +     +DCP+   T
Sbjct: 203 VNQQATRFVDCPTDDAT 213

[Close]

BLAST of MC08g1405.1 vs. NCBI nr
Match: XP_022159354.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 440 bits (1132), Expect = 8.82e-156
Identity = 203/218 (93.12%), Postives = 210/218 (96.33%), Query Frame = 0


Query: 1   MEKKGIALVLSTLVVIASCDYNYYQVTLQWQPATCSDTNLQSPCVNTPQDRFSIHGVWPT 60
           MEKKGI LV STLVVIASCDYNYYQVTLQWQPATCSD NLQ+PCVNTPQDRFSIHGVWPT
Sbjct: 1   MEKKGIVLVFSTLVVIASCDYNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSIHGVWPT 60

Query: 61  LSSGSYPQCPGTPAYDGSKISAIRNRLNTDWPSVIYGTNPILWSHEWTTHGTCSYPSLSQ 120
           LSSGSYPQCPGTPAYDGSKIS IRNRLN DWPSVIYGTNP LWSHEWTTHGTCSYPSLSQ
Sbjct: 61  LSSGSYPQCPGTPAYDGSKISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCSYPSLSQ 120

Query: 121 LDYFTFGLDTYAKYDILSALDKEDIYPSEDLYTKS-INDAIRKITQKLPGLRCNTNDYSR 180
           LDYFTFGLDTYAKYDI SALDKEDIYPSED+Y+KS IN+AIRKITQKLPGLRCNTNDYSR
Sbjct: 121 LDYFTFGLDTYAKYDIYSALDKEDIYPSEDVYSKSSINNAIRKITQKLPGLRCNTNDYSR 180

Query: 181 ERQLHEIVLCFAKSDRAIIDCPSSVVTCGNYVYWYPWY 217
           ERQLHEI+LCFAKS+RAIIDCPS+VVTCGNYVYWYPWY
Sbjct: 181 ERQLHEIILCFAKSNRAIIDCPSNVVTCGNYVYWYPWY 218

[Close]

BLAST of MC08g1405.1 vs. NCBI nr
Match: XP_038903348.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 248 bits (632), Expect = 1.04e-79
Identity = 118/219 (53.88%), Postives = 150/219 (68.49%), Query Frame = 0


Query: 1   MEKKGIALVLSTLVVIASC-----DYNYYQVTLQWQPATCSDTNLQSPCVNTPQDRFSIH 60
           M K+ I LVLS L+++        +++YYQV  QWQPA CS   L+SPCV  P++RF+IH
Sbjct: 1   MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIH 60

Query: 61  GVWPTLSSGSYPQCPGTPAYDGSKISAIRNRLNTDWPSVIYGTNPILWSHEWTTHGTCSY 120
           GVWP+L+SG+  +C GTP YDG+KI AIR+RL+ DWPSV+YGTNP LWSHEW THGTCS 
Sbjct: 61  GVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSN 120

Query: 121 PSLSQLDYFTFGLDTYAKYDILSALDKEDIYPSEDLYTKS-INDAIRKITQKLPGLRCNT 180
             L Q +YF FGL  + KY+++S L K  I P  + Y+   I  AI   T K P LRCN 
Sbjct: 121 YYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNK 180

Query: 181 NDYSRERQLHEIVLCFAKSDRAIIDCPSSVVTCGNYVYW 213
           N  S ++QL+EI LCF K +RA+ DCP + VTCGN   W
Sbjct: 181 NKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSW 219

[Close]

BLAST of MC08g1405.1 vs. NCBI nr
Match: XP_022158939.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 240 bits (612), Expect = 1.52e-76
Identity = 115/221 (52.04%), Postives = 144/221 (65.16%), Query Frame = 0


Query: 1   MEKKGIALVLSTLVVIASCD----YNYYQVTLQWQPATCSDTNLQSPCVNTPQDRFSIHG 60
           M K+ I LVLS  + I+S +    ++YYQV LQWQPATCS   L++ CV  P+DR+S+HG
Sbjct: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG 60

Query: 61  VWPTLSSGSYPQCPGTPAYDGSKISAIRNRLNTDWPSVIYGTNPILWSHEWTTHGTCSYP 120
           VWP+LSSG  P C GTP YD   I  I+ RL+ DWP+VI G NP  WS+EWT HGTCS  
Sbjct: 61  VWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSA 120

Query: 121 SLSQLDYFTFGLDTYAKYDILSALDKEDIYPSEDLYTKSI-NDAIRKITQKLPGLRCNTN 180
            L+Q  YF FGL  Y  YD+ S L +  IYP+   YTK++  +AI   T K P LRCN N
Sbjct: 121 YLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNN 180

Query: 181 DYSRERQLHEIVLCFAKSDRAIIDCPSSVVTCGNYVYWYPW 216
             +   QLHEI LCF+K+ + +IDCP +VVTC  Y  W  W
Sbjct: 181 YRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSW 221

[Close]

BLAST of MC08g1405.1 vs. NCBI nr
Match: XP_022156540.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 229 bits (584), Expect = 2.88e-72
Identity = 110/221 (49.77%), Postives = 144/221 (65.16%), Query Frame = 0


Query: 2   EKKGIALVLSTLVVIASCD----YNYYQVTLQWQPATCSDTNLQSPCVNTPQDRFSIHGV 61
           ++  I L+LS L++I   +    ++YYQV LQWQPATCS + L   CV  P +RFSIHGV
Sbjct: 3   KRSEIVLILSMLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSIHGV 62

Query: 62  WPTLSSGSYPQCPGTPAYDGSKI-SAIRNRLNTDWPSVIYGTNPILWSHEWTTHGTCSYP 121
           WP+L SG    C GTP YDG+++ S+IR RL+ DWPSV++  +P  WSHEWT HGTCSY 
Sbjct: 63  WPSLLSGD-AVCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTCSYA 122

Query: 122 SLSQLDYFTFGLDTYAKYDILSALDKEDIYPSEDLYTKS-INDAIRKITQKLPGLRCNTN 181
            L Q  YF FGL  Y  YD+L  L+  ++YP+ +LY +S + + I   T K P LRCN N
Sbjct: 123 YLDQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRCNNN 182

Query: 182 DYSRERQLHEIVLCFAKSDRAIIDCPSSVVTCGNYVYWYPW 216
            +S + QLHEI LCF K D ++ DCP + V C +Y  W  W
Sbjct: 183 FWSGDVQLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNSW 222

[Close]

BLAST of MC08g1405.1 vs. NCBI nr
Match: AEM42996.1 (S-RNase [Siraitia grosvenorii])

HSP 1 Score: 203 bits (517), Expect = 3.96e-62
Identity = 99/223 (44.39%), Postives = 140/223 (62.78%), Query Frame = 0


Query: 1   MEKKGIALVLSTLVVIASCD----YNYYQVTLQWQPATCSDTNLQSP-CVNTPQDRFSIH 60
           M K+ I LVLS LV+IASC+    ++Y QV +QWQPATCS +N   P C   P +R+SIH
Sbjct: 1   MAKREIVLVLSMLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSIH 60

Query: 61  GVWPTLSSGSYPQCPGTPAYDGSKISAIRNRLNTDWPSVIYGTNPILWSHEWTTHGTCSY 120
           GVWP+L SG+  QC G+P ++ + IS ++  ++  WP++I G N  LW HEW  HGTC+ 
Sbjct: 61  GVWPSLYSGAATQCSGSP-FNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTE 120

Query: 121 PSLSQLD-YFTFGLDTYAKYDILSALDKEDIYPSEDLYTKS-INDAIRKITQKLPGLRCN 180
             L   D YF+F +DTY  Y++L  L    I P+  +Y++  +  AIR  T K P +RCN
Sbjct: 121 EILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRCN 180

Query: 181 TNDYSRERQLHEIVLCFAKSDRAIIDCPSSVVTCGNYVYWYPW 216
            N ++ E+Q+HE+ LC+     A++DCP +   C NY  WY W
Sbjct: 181 YNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSW 222

[Close]

BLAST of MC08g1405.1 vs. ExPASy TrEMBL
Match: A0A6J1E266 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1)

HSP 1 Score: 440 bits (1132), Expect = 4.27e-156
Identity = 203/218 (93.12%), Postives = 210/218 (96.33%), Query Frame = 0


Query: 1   MEKKGIALVLSTLVVIASCDYNYYQVTLQWQPATCSDTNLQSPCVNTPQDRFSIHGVWPT 60
           MEKKGI LV STLVVIASCDYNYYQVTLQWQPATCSD NLQ+PCVNTPQDRFSIHGVWPT
Sbjct: 1   MEKKGIVLVFSTLVVIASCDYNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSIHGVWPT 60

Query: 61  LSSGSYPQCPGTPAYDGSKISAIRNRLNTDWPSVIYGTNPILWSHEWTTHGTCSYPSLSQ 120
           LSSGSYPQCPGTPAYDGSKIS IRNRLN DWPSVIYGTNP LWSHEWTTHGTCSYPSLSQ
Sbjct: 61  LSSGSYPQCPGTPAYDGSKISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCSYPSLSQ 120

Query: 121 LDYFTFGLDTYAKYDILSALDKEDIYPSEDLYTKS-INDAIRKITQKLPGLRCNTNDYSR 180
           LDYFTFGLDTYAKYDI SALDKEDIYPSED+Y+KS IN+AIRKITQKLPGLRCNTNDYSR
Sbjct: 121 LDYFTFGLDTYAKYDIYSALDKEDIYPSEDVYSKSSINNAIRKITQKLPGLRCNTNDYSR 180

Query: 181 ERQLHEIVLCFAKSDRAIIDCPSSVVTCGNYVYWYPWY 217
           ERQLHEI+LCFAKS+RAIIDCPS+VVTCGNYVYWYPWY
Sbjct: 181 ERQLHEIILCFAKSNRAIIDCPSNVVTCGNYVYWYPWY 218

[Close]

BLAST of MC08g1405.1 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 240 bits (612), Expect = 7.38e-77
Identity = 115/221 (52.04%), Postives = 144/221 (65.16%), Query Frame = 0


Query: 1   MEKKGIALVLSTLVVIASCD----YNYYQVTLQWQPATCSDTNLQSPCVNTPQDRFSIHG 60
           M K+ I LVLS  + I+S +    ++YYQV LQWQPATCS   L++ CV  P+DR+S+HG
Sbjct: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG 60

Query: 61  VWPTLSSGSYPQCPGTPAYDGSKISAIRNRLNTDWPSVIYGTNPILWSHEWTTHGTCSYP 120
           VWP+LSSG  P C GTP YD   I  I+ RL+ DWP+VI G NP  WS+EWT HGTCS  
Sbjct: 61  VWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSA 120

Query: 121 SLSQLDYFTFGLDTYAKYDILSALDKEDIYPSEDLYTKSI-NDAIRKITQKLPGLRCNTN 180
            L+Q  YF FGL  Y  YD+ S L +  IYP+   YTK++  +AI   T K P LRCN N
Sbjct: 121 YLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNNN 180

Query: 181 DYSRERQLHEIVLCFAKSDRAIIDCPSSVVTCGNYVYWYPW 216
             +   QLHEI LCF+K+ + +IDCP +VVTC  Y  W  W
Sbjct: 181 YRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSW 221

[Close]

BLAST of MC08g1405.1 vs. ExPASy TrEMBL
Match: A0A6J1DQK2 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1)

HSP 1 Score: 229 bits (584), Expect = 1.40e-72
Identity = 110/221 (49.77%), Postives = 144/221 (65.16%), Query Frame = 0


Query: 2   EKKGIALVLSTLVVIASCD----YNYYQVTLQWQPATCSDTNLQSPCVNTPQDRFSIHGV 61
           ++  I L+LS L++I   +    ++YYQV LQWQPATCS + L   CV  P +RFSIHGV
Sbjct: 3   KRSEIVLILSMLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSIHGV 62

Query: 62  WPTLSSGSYPQCPGTPAYDGSKI-SAIRNRLNTDWPSVIYGTNPILWSHEWTTHGTCSYP 121
           WP+L SG    C GTP YDG+++ S+IR RL+ DWPSV++  +P  WSHEWT HGTCSY 
Sbjct: 63  WPSLLSGD-AVCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTCSYA 122

Query: 122 SLSQLDYFTFGLDTYAKYDILSALDKEDIYPSEDLYTKS-INDAIRKITQKLPGLRCNTN 181
            L Q  YF FGL  Y  YD+L  L+  ++YP+ +LY +S + + I   T K P LRCN N
Sbjct: 123 YLDQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRCNNN 182

Query: 182 DYSRERQLHEIVLCFAKSDRAIIDCPSSVVTCGNYVYWYPW 216
            +S + QLHEI LCF K D ++ DCP + V C +Y  W  W
Sbjct: 183 FWSGDVQLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNSW 222

[Close]

BLAST of MC08g1405.1 vs. ExPASy TrEMBL
Match: K7NBR4 (S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 203 bits (517), Expect = 1.92e-62
Identity = 99/223 (44.39%), Postives = 140/223 (62.78%), Query Frame = 0


Query: 1   MEKKGIALVLSTLVVIASCD----YNYYQVTLQWQPATCSDTNLQSP-CVNTPQDRFSIH 60
           M K+ I LVLS LV+IASC+    ++Y QV +QWQPATCS +N   P C   P +R+SIH
Sbjct: 1   MAKREIVLVLSMLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSIH 60

Query: 61  GVWPTLSSGSYPQCPGTPAYDGSKISAIRNRLNTDWPSVIYGTNPILWSHEWTTHGTCSY 120
           GVWP+L SG+  QC G+P ++ + IS ++  ++  WP++I G N  LW HEW  HGTC+ 
Sbjct: 61  GVWPSLYSGAATQCSGSP-FNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTE 120

Query: 121 PSLSQLD-YFTFGLDTYAKYDILSALDKEDIYPSEDLYTKS-INDAIRKITQKLPGLRCN 180
             L   D YF+F +DTY  Y++L  L    I P+  +Y++  +  AIR  T K P +RCN
Sbjct: 121 EILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRCN 180

Query: 181 TNDYSRERQLHEIVLCFAKSDRAIIDCPSSVVTCGNYVYWYPW 216
            N ++ E+Q+HE+ LC+     A++DCP +   C NY  WY W
Sbjct: 181 YNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSW 222

[Close]

BLAST of MC08g1405.1 vs. ExPASy TrEMBL
Match: A0A6J1DYI5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025745 PE=3 SV=1)

HSP 1 Score: 184 bits (468), Expect = 4.50e-55
Identity = 102/220 (46.36%), Postives = 128/220 (58.18%), Query Frame = 0


Query: 1   MEKKGIALVLSTLVVIASCD---YNYYQVTLQWQPATCSDTNLQSPCVNTPQDRFSIHGV 60
           M K+ I  VL  L+ IAS +   ++YYQV  QWQPATC+D  L + CV  P +RFSIHGV
Sbjct: 1   MAKREIIFVLVLLLFIASSEQASFDYYQVVFQWQPATCNDITLPTRCVQNPDNRFSIHGV 60

Query: 61  WPTLSSGS-YPQCPGTPAYDGSKISAIRNRLNTDWPSVIYGTNPILWSHEWTTHGTCSYP 120
           WP+ +SGS    C GT A+D + I +IR  LN +WP VI   N + WS EW  HGTCS  
Sbjct: 61  WPSKTSGSRVGPCNGT-AFDPATIVSIRAGLNQNWPDVIRRKNQVFWSDEWRKHGTCSED 120

Query: 121 SLSQLDYFTFGLDTYAKY--DILSALDKEDIYPSEDLYTKS-INDAIRKITQKLPGLRCN 180
            L+QL YFTFGL+ Y     DI+  L K+ I P+   Y K+ +  AI  +  K  G RCN
Sbjct: 121 LLNQLQYFTFGLNIYHDEGKDIVEKLGKKKIIPNGKKYKKADVEKAISSVIGKRLGFRCN 180

Query: 181 TNDYSRERQLHEIVLCFAKSDRAIIDCPSSVVTCGNYVYW 213
             D S   QLHEI  C+AK    + DCPS+   C     W
Sbjct: 181 I-DQSNYHQLHEISFCYAKDASTLQDCPSNATNCPTKFKW 218

[Close]

BLAST of MC08g1405.1 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 120.6 bits (301), Expect = 1.6e-27
Identity = 65/187 (34.76%), Postives = 96/187 (51.34%), Query Frame = 0


Query: 20  DYNYYQVTLQWQPATC-SDTNLQSPCVNTPQDRFSIHGVWPTLSSGSYPQ-CPGTPAYDG 79
           D++++   LQW  A C S  +   P    P   F IHG+WP   +G +PQ C     +D 
Sbjct: 21  DFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDD 80

Query: 80  SKISAIRNRLNTDWPSVIYGTNPIL--WSHEWTTHGTCSYPSLSQLDYFTFGLDTYAKYD 139
            ++S + + L  +WP++   +N  +  W+HEW  HGTC+   L Q DYF  GL    K +
Sbjct: 81  LRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQKAN 140

Query: 140 ILSALDKEDIYPSEDLY-TKSINDAIRKITQKLPGLRCNTNDYSRERQLHEIVLCFAKSD 199
           +L AL    I P +  Y  K I + I+++    PG+ CN  D S   QL++I LC   S 
Sbjct: 141 LLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECN-KDSSHNSQLYQIYLCVDTSA 200

Query: 200 RAIIDCP 202
              I+CP
Sbjct: 201 SKFINCP 206

[Close]

BLAST of MC08g1405.1 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 115.2 bits (287), Expect = 6.8e-26
Identity = 67/207 (32.37%), Postives = 103/207 (49.76%), Query Frame = 0


Query: 9   VLSTLVVIASC--------DYNYYQVTLQWQPATCSDTNLQS--PCVNTPQDRFSIHGVW 68
           ++S LV++ S         D++++    QW P +  DT  +   P    P   F IHG+W
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQW-PGSYCDTQKKCCYPNSGKPAADFGIHGLW 69

Query: 69  PTLSSGSYP-QCPGTPAYDGSKISAIRNRLNTDWPSVI--YGTNPILWSHEWTTHGTCSY 128
           P    G+YP  C  +  +D S IS +   +   WP++    G+    W HEW  HGTCS 
Sbjct: 70  PNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSE 129

Query: 129 PSLSQLDYFTFGLDTYAKYDILSALDKEDIYPSEDLYT-KSINDAIRKITQKLPGLRCNT 188
             + Q +YF   L+   K ++L AL K  I P    Y+ +SI D+I++     P + CN 
Sbjct: 130 SVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECN- 189

Query: 189 NDYSRERQLHEIVLCFAKSDRAIIDCP 202
            D S   QL+++ LC  +S   +I+CP
Sbjct: 190 RDGSGNSQLYQVYLCVDRSGSGLIECP 214

[Close]

BLAST of MC08g1405.1 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 113.6 bits (283), Expect = 2.0e-25
Identity = 70/213 (32.86%), Postives = 98/213 (46.01%), Query Frame = 0


Query: 1   MEKKGIALVLSTLVVIASC-------DYNYYQVTLQWQPATCSDTNLQS-PCVNTPQDRF 60
           M  KG   VL  L+V           D++++   LQW  A C  +     P    P   F
Sbjct: 1   MGAKGCVNVLLKLLVFQGLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADF 60

Query: 61  SIHGVWPTLSSGSYP-QCPGTPAYDGSKISAIRNRLNTDWPSVIYGTNP--ILWSHEWTT 120
            IHG+WP  + GS+P  C     +D S+IS + + L  +WP++   +N     W HEW  
Sbjct: 61  GIHGLWPNYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEK 120

Query: 121 HGTCSYPSLSQLDYFTFGLDTYAKYDILSALDKEDIYPSEDLYT-KSINDAIRKITQKLP 180
           HGTCS   + Q +YF   L    K ++L  L    I P +  Y    I +AI+      P
Sbjct: 121 HGTCSESVMDQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTP 180

Query: 181 GLRCNTNDYSRERQLHEIVLCFAKSDRAIIDCP 202
           G+ CN  D  R  QLH+I +C   S    I+CP
Sbjct: 181 GIECN-KDPERNAQLHQIYICVDTSGTEFIECP 212

[Close]

BLAST of MC08g1405.1 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 80.5 bits (197), Expect = 1.8e-15
Identity = 56/199 (28.14%), Postives = 88/199 (44.22%), Query Frame = 0


Query: 20  DYNYYQVTLQWQPATCSDTN---LQSPCV--NTPQDRFSIHGVWPTLSSGSYPQCPGTPA 79
           +++Y+ ++LQW    C  T     ++ C   +    +F+IHG+WP  + GS+P C     
Sbjct: 31  EFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSD 90

Query: 80  YDGSKISAIRNRLNTDWPSVIYGT-------NPILWSHEWTTHGTCSYPSL-SQLDYFTF 139
           +   +IS + + L   WPS+  G+           W HEW  HGTCS P    + +YF  
Sbjct: 91  FKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLT 150

Query: 140 GLDTYAKYDILSALDKEDIYP--SEDLYTKSINDAIRKITQKLPGLRCNTNDYSRERQLH 199
            L+ Y K+++   L +       SE      I  AI+      P + C      +   + 
Sbjct: 151 TLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVC------KRDAID 210

Query: 200 EIVLCFAKSDRAIIDCPSS 204
           EI +CF K D    DC  S
Sbjct: 211 EIRICFYK-DFKPRDCVGS 222

The following BLAST results are available for this feature:

BLAST of MC08g1405.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	2.6e-30	37.63	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
Q7M438	3.9e-26	32.86	Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3	[more]
P42813	9.5e-25	32.37	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80196	1.1e-23	34.98	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
P83618	3.2e-20	34.01	Ribonuclease-like storage protein OS=Panax ginseng OX=4054 PE=1 SV=2	[more]

BLAST of MC08g1405.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159354.1	8.82e-156	93.12	ribonuclease MC-like [Momordica charantia]	[more]
XP_038903348.1	1.04e-79	53.88	ribonuclease MC-like [Benincasa hispida]	[more]
XP_022158939.1	1.52e-76	52.04	ribonuclease MC-like [Momordica charantia]	[more]
XP_022156540.1	2.88e-72	49.77	ribonuclease MC-like [Momordica charantia]	[more]
AEM42996.1	3.96e-62	44.39	S-RNase [Siraitia grosvenorii]	[more]

BLAST of MC08g1405.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E266	4.27e-156	93.12	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1	[more]
A0A6J1E0X1	7.38e-77	52.04	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1	[more]
A0A6J1DQK2	1.40e-72	49.77	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1	[more]
K7NBR4	1.92e-62	44.39	S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]
A0A6J1DYI5	4.50e-55	46.36	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025745 PE=3 SV=1	[more]

BLAST of MC08g1405.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26820.1	1.6e-27	34.76	ribonuclease 3	[more]
AT2G02990.1	6.8e-26	32.37	ribonuclease 1	[more]
AT1G14220.1	2.0e-25	32.86	Ribonuclease T2 family protein	[more]
AT2G39780.1	1.8e-15	28.14	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 22..200 e-value: 9.1E-39 score: 133.5
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 12..202
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 15..215 e-value: 2.1E-50 score: 173.6
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 21..212
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 12..202
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..19 score: 6.0
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 102..113
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 21..207 e-value: 2.14831E-57 score: 178.295

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC08g1405	MC08g1405	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC08g1405.1-cds	MC08g1405.1-cds-MC08:15313756..15313944	CDS
MC08g1405.1-cds	MC08g1405.1-cds-MC08:15313947..15314171	CDS
MC08g1405.1-cds	MC08g1405.1-cds-MC08:15314254..15314316	CDS
MC08g1405.1-cds	MC08g1405.1-cds-MC08:15314330..15314503	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC08g1405.1	MC08g1405.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding