MC08g0476.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC08g0476.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	thermospermine synthase ACAULIS5
Location	MC08: 3895090 .. 3899072 (+)
Sequence length	1779
RNA-Seq Expression	MC08g0476.1
Synteny	MC08g0476.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAGTGGGCTTCAAAAATAATAATAAAAAAAATTAAATTAAAATCCCCTGCAGGCTGCAGCTATAAAATGATCTCACCACCATCTCCAGAAATCCCCCATCAACTTCCACCTCCCTGTTTCATAGACCTCTGCCTCTCACTCCCATTTCTCATTCTCCTTCTTCGCTCCTAATTACCTCACCACCAACGCCATGGGCCCGGCAGTTCTTAGTTCCCACACCAATGGCTTCTCAAAATTCGACGACAAATCCAATTACATCAATGGCAATTCTTCTCACGAAGCAGTAATCAGCAATGGCAGCGATTGCCATTGGTTCGAAGAAATCATCGACGATAACCTAAAATGGTCCTTCGCACTGAACAGGTATTGATTTGCTTGCATATTTCCAGATCTTCTCTGAATTTCAGCGGCGATTCATAATCCCTCTCTTCTTTTCAGCGTTCTGCAGAAGGGAACCAGCGAGTTCCAGGACATCGCTCTGTTAGACACCAAGCGTTTTGGCAAGGTGAGTTTATGAAGTTCCGAATTCAGATTCTGTTTGGCTATTTTCCACGATTTCTGAAAATTTTCCCGGAAAATCGGTTCCGTTTCCGTTCAATTCGGTCGGAACCGGATAAGATCATCTGATTAACATTTGTGAGACGGCTGAGTGCAGGCATTGATGATCGACGGAAAGATGCAGAGTGCTGAAGCCGATGAATTCGTGTATCATGAAAGCTTGATTCATCCACCCCTATTATGCCACTCAAAGTTCGTCTTCCATCTCTGTTTTCATGTTCACGGATAATTCTTTCAGTTTATTTTTAATATTAAATGAAATATATTAGAAAATGTCTCACATGATAATGTTTTTAATTTAACTTAATGTCACGGAGTTGTATTTTTATAGTCCATCTAAACAAACATTAATCAAACCTTTAATTAGTGTAAAGAGTTTGTAAATTATATTGCTAATATGATCAACATGAGAATGACTCACAATATTTGGACATACTCATTTTTCGTAGGTTCAAATTTCCATACTCCGTACTAAAGAAAATAATTTATATATCATACTATTCAAACAGTTAGTTTTAATGAAGTTAATATATATGATATATTTATAGCAATTTATTTTGCAAGCTATTGAGAGTACTTACATGCTATTCATAAAGTACATAAACTAGAAAGCCGTCAACTTGAGCATAATTCAAAAAAAAAGGAAAAAACTATAGAGTTAACAATCTATACTTACACCTTTCTTTTTTTTCAGAAACTAACTTATGAAAAAAGGAATAATCAAGGTATAAAATAAAATATGAAACTTCTGGTTTTGGAATTAACTTTATCTTCATTTTTTTTTTTAATTTTTGCAGCCCGAAAAATGTATTCATAATGGGAGGTGGGGAAGGTTCAGCAGCAAGGGAAGTCCTCAAACATAAATCAACAGAGAAAGTTGTTATGTGCGATATTGATCAGGTAATTGTATTCATTAAATAATTAAAACATTCATTCAGTTTAATTAATCAATTAATTAATTTTTTATTCGATCCACTTCCCTGGTTACTTGCAGGATGTGGTGGATTTCTGCCGCAAGCATTTGACTGTGAATCAGGAAGCATTTTGTCACAAGAAGCTTCATCTTCTAATAAACGATGCCAGGTAAATTAGTTACTTTGTTTTTCTATTATTTAAAAAATTAGAGTCAGATTTCGAAAACTATAAGAACATAGTTCAAAAATTATTTTTTATTTGTACTAAAAAATATATATTTTTTAAAATTTTGTTAAAATTTCAAGAGTATTTTTAGAAATTGAACAAAAGAAAGAAATTAAGTTTTGTTTCTTAAAACAAAAATTCTATACAATCATGTTTGGATAACTTTTAAATTTTTAAATTTTCTTTTAGTGCAATTTCATTTATGGACCTTTTTCTTTTTTATATATATATAAATTTATGAATGAGGGAACTTTTTATTCCTTTCAAAATTATAAATTTGTAAATAGCAAATTCAACCTATACCATAACAATAGTTCTAATAGTTCTAATGATTCTATTACCCTTTCAAAAAACAATCTTTAAAACAAAAAACAGAAAAATATTTTGAGAACAAATCCATATATTGAAAAAAGTTAACTGGTAAAAATATAGAGATGATAAAATAAAATTATCACACACGTGCTTAAAAGTTTAATATTCTAAATCGAACCGACAATTAGGAGTGTTCGTTTTTTTCGTGGCCAAAGATGGAGAACATTTTGTCCCATCACTCAAATAGGGAAGGAGATGAAAAATAACAAAATGGAGATAAAGTGTTCCACATCCGGCTCCGCTCCACCCTTTTAATATATATATTTATTAGATGGTTTTAGAAATTTTTTTTCCCAACTAAATGAGATTCTACGCAATAATTTAATTATGTAAATGGAGATTTGAAATTTATTTTTAAAAATTATTTAATTAAAAGATAAGCTGGGAATAATTTCTTCATTTGTTGACAAAAGGGCCGAATTGGAAACGAGAAACGAGAAATTTGATGTCATAATCGGAGACCTAGCAGACCCAGTTAAAGATGGACCTTGTTACAAGCTCTACACCAAATCCTTTTACCAGGAAATCGTCAAACCAAAGCTCCATGACAATGGCATCTTCGTAACTCAGGTTTGCCAATCTTTTATTGCCAAAATTAGTGCATTAAAACAGGCAAATTTTACACAAGGTTTGACAAATTTAAGGTTTTTCAGGCAGGACCAGCCGGCATTTTCACCCACAAAGAGGTTTTTACCTCAATTTACAACACAATCAAACAGACCTTCAAGTGTAAGTATCTCTCAGCAGTGAATAATTAAGCCATTTTGTGTGAAGCCAATGTCAGATTTCATAACCCTTTTCTCTCTGTTTGCTGCTGCAGATGTGATTGCTTACACGGCCTACATACCATCTTTTGCAGACACATGGGGCTGGGTTATGGTATGAGAAAAATTAGAAGCTTATTTGAACTTGTTGGCTGAAACAGAAAATTAGTCTGGTTTTTTTGTTACTCGTGAGCTGAGTTTTCTGTTTGAAAAACAGGCATCGGATCATCCTTTCTGTATAAAAGCTGAGGAAATGGATAAGAGAATTCAGGAAAGAATCGATGGAGAGTTGCTCTATTTAACCGGTGCCTCAATCGTCTCCTCCACCATTATGAACAAGATTGTTTCTTTAGCGTAAGTCCATCATAATTTCTCCCAAAACTGATTAAAGGTATAAAAGATTCATAAAGTTATAAGGATAACAGTTCGAGTAATAAACAAAACAAAAGATGAAAACTCTGTGTGTTCTACATTGATTTATAGCATTGTTTCATTCAACCTGTTTGACATCCTCAGGTTGTTGAATGAAACTCACATCTACACTGAGGCGACTGCAAAATTCATACACGGCCACGGGGTTGGATTCAAGCAACTGAACGGTGGGAGTTGCCGCGTATGAAGAAGAACAAAAACAAACACTTTAGAGGTGGTCTTTTCCCAGATTAAGAAAACCAATCAACATTTGTACTGTGAACTATTTGGTTTAGATGGTGTAAAACTAGCTCTAACAACACGTGGGAGTGGTAATTCAAATCTCGAACCTTTTAGTAAGAGAATTTAAGTCTTAACCAATAGAGCTACGCTAGCTTTTATTGATTATCATCCCGTTTCATATATAATTTAGTTTATATGGTTTGGTTAAATGTCACGAATCATCTTTGTCATTATCTCTGTTTCATATGTGATTAGTATTACACTACTGGTAGGGATCCAGGAGCGACTTAGTCTGAATTTCATCAACCCCTTGTATGATCTGTTTGGAAAGGAATTAATAGTCATTTCAACTTTCAGATTTGGTTCCACATTCTGTTTTGGAAATCCCTTCCTTCTGTGTGCAAAGGTAAACAAAAATATTGGATATTACTGGTGTAATGGATATATTAGTGTGATGATCTAGATAAGCTCAAATTATTCTGCAAGGTTTTCA

mRNA sequence

AAAAGTGGGCTTCAAAAATAATAATAAAAAAAATTAAATTAAAATCCCCTGCAGGCTGCAGCTATAAAATGATCTCACCACCATCTCCAGAAATCCCCCATCAACTTCCACCTCCCTGTTTCATAGACCTCTGCCTCTCACTCCCATTTCTCATTCTCCTTCTTCGCTCCTAATTACCTCACCACCAACGCCATGGGCCCGGCAGTTCTTAGTTCCCACACCAATGGCTTCTCAAAATTCGACGACAAATCCAATTACATCAATGGCAATTCTTCTCACGAAGCAGTAATCAGCAATGGCAGCGATTGCCATTGGTTCGAAGAAATCATCGACGATAACCTAAAATGGTCCTTCGCACTGAACAGCGTTCTGCAGAAGGGAACCAGCGAGTTCCAGGACATCGCTCTGTTAGACACCAAGCGTTTTGGCAAGGCATTGATGATCGACGGAAAGATGCAGAGTGCTGAAGCCGATGAATTCGTGTATCATGAAAGCTTGATTCATCCACCCCTATTATGCCACTCAAACCCGAAAAATGTATTCATAATGGGAGGTGGGGAAGGTTCAGCAGCAAGGGAAGTCCTCAAACATAAATCAACAGAGAAAGTTGTTATGTGCGATATTGATCAGGATGTGGTGGATTTCTGCCGCAAGCATTTGACTGTGAATCAGGAAGCATTTTGTCACAAGAAGCTTCATCTTCTAATAAACGATGCCAGGGCCGAATTGGAAACGAGAAACGAGAAATTTGATGTCATAATCGGAGACCTAGCAGACCCAGTTAAAGATGGACCTTGTTACAAGCTCTACACCAAATCCTTTTACCAGGAAATCGTCAAACCAAAGCTCCATGACAATGGCATCTTCGTAACTCAGGCAGGACCAGCCGGCATTTTCACCCACAAAGAGGTTTTTACCTCAATTTACAACACAATCAAACAGACCTTCAAGTATGTGATTGCTTACACGGCCTACATACCATCTTTTGCAGACACATGGGGCTGGGTTATGGCATCGGATCATCCTTTCTGTATAAAAGCTGAGGAAATGGATAAGAGAATTCAGGAAAGAATCGATGGAGAGTTGCTCTATTTAACCGGTGCCTCAATCGTCTCCTCCACCATTATGAACAAGATTGTTTCTTTAGCGTTGTTGAATGAAACTCACATCTACACTGAGGCGACTGCAAAATTCATACACGGCCACGGGGTTGGATTCAAGCAACTGAACGGTGGGAGTTGCCGCGTATGAAGAAGAACAAAAACAAACACTTTAGAGGTGGTCTTTTCCCAGATTAAGAAAACCAATCAACATTTGTACTGTGAACTATTTGGTTTAGATGGTGTAAAACTAGCTCTAACAACACGTGGGAGTGGTAATTCAAATCTCGAACCTTTTAGTAAGAGAATTTAAGTCTTAACCAATAGAGCTACGCTAGCTTTTATTGATTATCATCCCGTTTCATATATAATTTAGTTTATATGGTTTGGTTAAATGTCACGAATCATCTTTGTCATTATCTCTGTTTCATATGTGATTAGTATTACACTACTGGTAGGGATCCAGGAGCGACTTAGTCTGAATTTCATCAACCCCTTGTATGATCTGTTTGGAAAGGAATTAATAGTCATTTCAACTTTCAGATTTGGTTCCACATTCTGTTTTGGAAATCCCTTCCTTCTGTGTGCAAAGGTAAACAAAAATATTGGATATTACTGGTGTAATGGATATATTAGTGTGATGATCTAGATAAGCTCAAATTATTCTGCAAGGTTTTCA

Coding sequence (CDS)

ATGGGCCCGGCAGTTCTTAGTTCCCACACCAATGGCTTCTCAAAATTCGACGACAAATCCAATTACATCAATGGCAATTCTTCTCACGAAGCAGTAATCAGCAATGGCAGCGATTGCCATTGGTTCGAAGAAATCATCGACGATAACCTAAAATGGTCCTTCGCACTGAACAGCGTTCTGCAGAAGGGAACCAGCGAGTTCCAGGACATCGCTCTGTTAGACACCAAGCGTTTTGGCAAGGCATTGATGATCGACGGAAAGATGCAGAGTGCTGAAGCCGATGAATTCGTGTATCATGAAAGCTTGATTCATCCACCCCTATTATGCCACTCAAACCCGAAAAATGTATTCATAATGGGAGGTGGGGAAGGTTCAGCAGCAAGGGAAGTCCTCAAACATAAATCAACAGAGAAAGTTGTTATGTGCGATATTGATCAGGATGTGGTGGATTTCTGCCGCAAGCATTTGACTGTGAATCAGGAAGCATTTTGTCACAAGAAGCTTCATCTTCTAATAAACGATGCCAGGGCCGAATTGGAAACGAGAAACGAGAAATTTGATGTCATAATCGGAGACCTAGCAGACCCAGTTAAAGATGGACCTTGTTACAAGCTCTACACCAAATCCTTTTACCAGGAAATCGTCAAACCAAAGCTCCATGACAATGGCATCTTCGTAACTCAGGCAGGACCAGCCGGCATTTTCACCCACAAAGAGGTTTTTACCTCAATTTACAACACAATCAAACAGACCTTCAAGTATGTGATTGCTTACACGGCCTACATACCATCTTTTGCAGACACATGGGGCTGGGTTATGGCATCGGATCATCCTTTCTGTATAAAAGCTGAGGAAATGGATAAGAGAATTCAGGAAAGAATCGATGGAGAGTTGCTCTATTTAACCGGTGCCTCAATCGTCTCCTCCACCATTATGAACAAGATTGTTTCTTTAGCGTTGTTGAATGAAACTCACATCTACACTGAGGCGACTGCAAAATTCATACACGGCCACGGGGTTGGATTCAAGCAACTGAACGGTGGGAGTTGCCGCGTATGA

Protein sequence

MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV

Homology

[Close]

BLAST of MC08g0476.1 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 2.4e-142
Identity = 239/345 (69.28%), Postives = 281/345 (81.45%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL 60
           MG AV     NGF +           S  + + SN  DCHW+EE IDD+LKWSFALNSVL
Sbjct: 1   MGEAVEVMFGNGFPEIH------KATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVL 60

Query: 61  QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG 120
            +GTSE+QDIALLDTKRFGK L+IDGKMQSAE DEF+YHE LIHP LL H NPK VFIMG
Sbjct: 61  HQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMG 120

Query: 121 GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE 180
           GGEGSAARE+LKH + EKVVMCDIDQ+VVDFCR+ LTVN +AFC+KKL L+I DA+AELE
Sbjct: 121 GGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELE 180

Query: 181 TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV 240
            R EKFD+I+GDLADPV+ GPCY+LYTKSFYQ I+KPKL  NGIFVTQAGPAGIFTHKEV
Sbjct: 181 KREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEV 240

Query: 241 FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY 300
           FTSIYNT+KQ FKYV AYTA++PSFADTWGWVMASDH F ++ +EMD+RI+ER++GEL+Y
Sbjct: 241 FTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMY 300

Query: 301 LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQL 346
           L   S VS+  +NK +SLAL  ET +Y+E  A+FIHGHGV ++ +
Sbjct: 301 LNAPSFVSAATLNKTISLALEKETEVYSEENARFIHGHGVAYRHI 339

[Close]

BLAST of MC08g0476.1 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.7e-55
Identity = 120/292 (41.10%), Postives = 170/292 (58.22%), Query Frame = 0


Query: 40  HWFEEIIDDNLKWSFALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYH 99
           HW  E          A+  V+ +  + +Q   L++  RFGKAL++DGK+QS   DEF+YH
Sbjct: 5   HWHIEWQTPYEFHGHAITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYH 64

Query: 100 ESLIHPPLLCHSNPKNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTV- 159
           E+L+HP LL  +NP+ + I+GGGEG+  REVLKHK+ + V M DID  V+DF +K+L   
Sbjct: 65  EALVHPLLLSINNPEKILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEW 124

Query: 160 NQEAFCHKKLHLLINDARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPK 219
           +Q AF + K  L+I D    ++   EKFD ++ DL DP+KD P   LYTK FY+E+   +
Sbjct: 125 HQGAFDNPKSKLVIEDGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KR 184

Query: 220 LHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASD-- 279
           +   GI      P+      E F+ IYNTIK  FK V A   Y+P+F   WG+V ASD  
Sbjct: 185 ISKWGIVTQATSPS---FSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEV 244

Query: 280 HPFCIKAEEMDKRIQERIDGELLYLTGASIVSSTIMNKIVSLALLNETHIYT 329
           +P     EE++ RI+ERIDG L +  G + ++   + K +   L  E  I T
Sbjct: 245 NPAEFSKEEINNRIKERIDGSLRFYDGETHITMFSIPKHIREVLEKENKIST 291

[Close]

BLAST of MC08g0476.1 vs. ExPASy Swiss-Prot
Match: Q9UXE4 (Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=speE PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.6e-53
Identity = 120/292 (41.10%), Postives = 169/292 (57.88%), Query Frame = 0


Query: 40  HWFEEIIDDNLKWSFALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYH 99
           HW  E           +  VL +  + +Q + L++  RFGK L+IDGK+QS   DE +YH
Sbjct: 5   HWLLEWQTPYEFHGHLIEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYH 64

Query: 100 ESLIHPPLLCHSN-PKNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHL-T 159
           E L+HP LL  +  PK+V I+GGGEG+  REVLK+KS EK VM DID+ V++F +K+L  
Sbjct: 65  ELLVHPLLLSLTKPPKSVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYE 124

Query: 160 VNQEAFCHKKLHLLINDARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKP 219
            +Q AF  K+ +L+I D    ++   EK+D II DL DP+K+   Y LYTK FY+++ + 
Sbjct: 125 WHQGAFEDKRTNLVITDGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYEKL-RE 184

Query: 220 KLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDH 279
            L++ G  VTQA         EV+ +IYNTIK+ FK   A   Y+ SF   WG+V     
Sbjct: 185 ILNERGGIVTQATSPSF--SLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGVR 244

Query: 280 PFCIKAEEMDKRIQERIDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTE 330
           P  +  +E+D +I ERIDG+L +  G S   S  + K +       T I TE
Sbjct: 245 PDLLSEDEVDSKINERIDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTE 293

[Close]

BLAST of MC08g0476.1 vs. ExPASy Swiss-Prot
Match: Q72K55 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=speE PE=3 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.9e-51
Identity = 113/288 (39.24%), Postives = 174/288 (60.42%), Query Frame = 0


Query: 56  LNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKN 115
           +  V+  G + FQD  L ++K FGK L++D  +QS E DE++YHE+L+HP +L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 116 VFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTV-NQEAFCHKKLHLLIND 175
           V I+GGGEG+  REVLKH + EK VM DID ++V+  ++H+   +Q AF   +  L+I+D
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 176 ARAELETRNEKFDVIIGDLADPV-KDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 235
           ARA LE   E++DV+I DL DPV +D P   LYT  FY+ +VK  L+  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMI- 200

Query: 236 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASD--HPFCIKAEEMDKRIQ 295
           + TH  V   ++ T+++ F+YV +Y  +IP F   +G+++ASD   P       ++ RI+
Sbjct: 201 LLTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIR 260

Query: 296 ERIDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHG 340
           ER +  L +LT   + +  ++ K +  AL  ET + T+    ++   G
Sbjct: 261 ER-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEG 305

[Close]

BLAST of MC08g0476.1 vs. ExPASy Swiss-Prot
Match: Q5SK28 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=speE PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.9e-51
Identity = 113/288 (39.24%), Postives = 174/288 (60.42%), Query Frame = 0


Query: 56  LNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKN 115
           +  V+  G + FQD  L ++K FGK L++D  +QS E DE++YHE+L+HP +L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 116 VFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTV-NQEAFCHKKLHLLIND 175
           V I+GGGEG+  REVLKH + EK VM DID ++V+  ++H+   +Q AF   +  L+I+D
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 176 ARAELETRNEKFDVIIGDLADPV-KDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 235
           ARA LE   E++DV+I DL DPV +D P   LYT  FY+ +VK  L+  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMI- 200

Query: 236 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASD--HPFCIKAEEMDKRIQ 295
           + TH  V   ++ T+++ F+YV +Y  +IP F   +G+++ASD   P       ++ RI+
Sbjct: 201 LLTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIR 260

Query: 296 ERIDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHG 340
           ER +  L +LT   + +  ++ K +  AL  ET + T+    ++   G
Sbjct: 261 ER-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEG 305

[Close]

BLAST of MC08g0476.1 vs. NCBI nr
Match: XP_022131411.1 (thermospermine synthase ACAULIS5 [Momordica charantia])

HSP 1 Score: 718 bits (1854), Expect = 1.97e-261
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL 60
           MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL
Sbjct: 1   MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL 60

Query: 61  QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG 120
           QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG
Sbjct: 61  QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG 120

Query: 121 GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE 180
           GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE
Sbjct: 121 GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE 180

Query: 181 TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV 240
           TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV
Sbjct: 181 TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV 240

Query: 241 FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY 300
           FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY
Sbjct: 241 FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY 300

Query: 301 LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV 352
           LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV
Sbjct: 301 LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV 352

[Close]

BLAST of MC08g0476.1 vs. NCBI nr
Match: KAG6598599.1 (Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 650 bits (1676), Expect = 3.59e-234
Identity = 319/358 (89.11%), Postives = 338/358 (94.41%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVIS--NGSDCHWFEEIIDDNLKWS 60
           MGPAVLSSHTNGFSK     F++KSNYING+SSHE+VI+  NG+DC+WFEEIID+NLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120

Query: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180
           K VFIMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRK+LTVNQE FCHKKLHL+IN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180

Query: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300
           IFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++R
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300

Query: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351
           IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTE TAKFIHG GVGFKQ NGGSCR
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCR 358

[Close]

BLAST of MC08g0476.1 vs. NCBI nr
Match: XP_022961616.1 (thermospermine synthase ACAULIS5 [Cucurbita moschata])

HSP 1 Score: 647 bits (1669), Expect = 4.19e-233
Identity = 318/358 (88.83%), Postives = 338/358 (94.41%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVIS--NGSDCHWFEEIIDDNLKWS 60
           MGPAVLSSHTNGFSK     F++K+NYING+SSHE+VI+  NG+DC+WFEEIID+NLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKNNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120

Query: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180
           K VFIMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRK+LTVNQE FCHKKLHL+IN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180

Query: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300
           IFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++R
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300

Query: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351
           IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTEATAKFIHG GVGFKQ NG SCR
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTEATAKFIHGQGVGFKQQNGRSCR 358

[Close]

BLAST of MC08g0476.1 vs. NCBI nr
Match: XP_022997196.1 (thermospermine synthase ACAULIS5-like [Cucurbita maxima])

HSP 1 Score: 646 bits (1667), Expect = 8.45e-233
Identity = 316/358 (88.27%), Postives = 338/358 (94.41%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVISN--GSDCHWFEEIIDDNLKWS 60
           MGPAVLSSHTNGFSK     F++KSNYING+SSHE+VI+N  G+DC+WFEEIID+NLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINNVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120

Query: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180
           K VFIMGGGEGSAAREVLKH+S EKVVMCDIDQDVVDFCRK+LTVNQE FCHKKLHL+IN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHRSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180

Query: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300
           IFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+E++DK+I++R
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEDLDKKIEDR 300

Query: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351
           IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTE TAKFIHG GVGFKQ NGGSCR
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCR 358

[Close]

BLAST of MC08g0476.1 vs. NCBI nr
Match: XP_023546524.1 (thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 646 bits (1666), Expect = 1.20e-232
Identity = 317/358 (88.55%), Postives = 336/358 (93.85%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVIS--NGSDCHWFEEIIDDNLKWS 60
           MGPAVLSSHTNGFSK     F++KSNYING+SSHE+VI+  NG+DC+WFEEIID+NLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120

Query: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180
           K VFIMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRK+LTVN E FCHKKLHL+IN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNHETFCHKKLHLVIN 180

Query: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300
           IFTHKEVFTSIYNTIK TF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++R
Sbjct: 241 IFTHKEVFTSIYNTIKHTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300

Query: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351
           IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTE TAKFIHG GVGFKQ NGGSCR
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCR 358

[Close]

BLAST of MC08g0476.1 vs. ExPASy TrEMBL
Match: A0A6J1BPF9 (thermospermine synthase ACAULIS5 OS=Momordica charantia OX=3673 GN=LOC111004633 PE=3 SV=1)

HSP 1 Score: 718 bits (1854), Expect = 9.53e-262
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL 60
           MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL
Sbjct: 1   MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL 60

Query: 61  QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG 120
           QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG
Sbjct: 61  QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG 120

Query: 121 GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE 180
           GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE
Sbjct: 121 GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE 180

Query: 181 TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV 240
           TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV
Sbjct: 181 TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV 240

Query: 241 FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY 300
           FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY
Sbjct: 241 FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY 300

Query: 301 LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV 352
           LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV
Sbjct: 301 LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV 352

[Close]

BLAST of MC08g0476.1 vs. ExPASy TrEMBL
Match: A0A6J1HAV2 (thermospermine synthase ACAULIS5 OS=Cucurbita moschata OX=3662 GN=LOC111462336 PE=3 SV=1)

HSP 1 Score: 647 bits (1669), Expect = 2.03e-233
Identity = 318/358 (88.83%), Postives = 338/358 (94.41%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVIS--NGSDCHWFEEIIDDNLKWS 60
           MGPAVLSSHTNGFSK     F++K+NYING+SSHE+VI+  NG+DC+WFEEIID+NLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKNNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120

Query: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180
           K VFIMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRK+LTVNQE FCHKKLHL+IN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180

Query: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300
           IFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++R
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300

Query: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351
           IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTEATAKFIHG GVGFKQ NG SCR
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTEATAKFIHGQGVGFKQQNGRSCR 358

[Close]

BLAST of MC08g0476.1 vs. ExPASy TrEMBL
Match: A0A6J1K6U5 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111492169 PE=3 SV=1)

HSP 1 Score: 646 bits (1667), Expect = 4.09e-233
Identity = 316/358 (88.27%), Postives = 338/358 (94.41%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVISN--GSDCHWFEEIIDDNLKWS 60
           MGPAVLSSHTNGFSK     F++KSNYING+SSHE+VI+N  G+DC+WFEEIID+NLKWS
Sbjct: 1   MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINNVNGNDCYWFEEIIDENLKWS 60

Query: 61  FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120
           FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61  FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120

Query: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180
           K VFIMGGGEGSAAREVLKH+S EKVVMCDIDQDVVDFCRK+LTVNQE FCHKKLHL+IN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHRSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180

Query: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
           DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240

Query: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300
           IFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+E++DK+I++R
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEDLDKKIEDR 300

Query: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351
           IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTE TAKFIHG GVGFKQ NGGSCR
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCR 358

[Close]

BLAST of MC08g0476.1 vs. ExPASy TrEMBL
Match: A0A1S3BCH1 (thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV=1)

HSP 1 Score: 634 bits (1636), Expect = 2.42e-228
Identity = 315/359 (87.74%), Postives = 336/359 (93.59%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSK-----FDDKSNYING-NSSHEAVISN--GSDCHWFEEIIDDNLKW 60
           MGPAVLSSHTNGFS+     F++K+NYIN  NSSHE+VI+N  G DCHW+EEIID+NLKW
Sbjct: 1   MGPAVLSSHTNGFSQSVDRNFEEKTNYINDDNSSHESVINNVNGDDCHWYEEIIDENLKW 60

Query: 61  SFALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSN 120
           SFALN VLQ+GTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHE LIHPPLLCHSN
Sbjct: 61  SFALNRVLQQGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHECLIHPPLLCHSN 120

Query: 121 PKNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLI 180
           PK+VFIMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRKHLTVN+EAFCHKKLHL+I
Sbjct: 121 PKSVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKHLTVNREAFCHKKLHLVI 180

Query: 181 NDARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPA 240
           NDAR ELE R EK+DVIIGDLADPVKDGPCYKLYTKSFY +I+KPKLH NGIFVTQAGPA
Sbjct: 181 NDARVELEKRKEKYDVIIGDLADPVKDGPCYKLYTKSFYHDILKPKLHHNGIFVTQAGPA 240

Query: 241 GIFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQE 300
           GIFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++
Sbjct: 241 GIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIED 300

Query: 301 RIDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGV-GFKQLNGGSC 350
           RI+GELLYLTG SIVSSTI+NKIVSLALLNETHIYTEATAKFIHG GV G KQLNGGSC
Sbjct: 301 RINGELLYLTGPSIVSSTIINKIVSLALLNETHIYTEATAKFIHGQGVVGCKQLNGGSC 359

[Close]

BLAST of MC08g0476.1 vs. ExPASy TrEMBL
Match: A0A0A0KIK6 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G362930 PE=3 SV=1)

HSP 1 Score: 618 bits (1594), Expect = 3.48e-222
Identity = 309/354 (87.29%), Postives = 325/354 (91.81%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKFDDKSNYING-NSSHEAVISN--GSDCHWFEEIIDDNLKWSFALN 60
           MGPAVLSSHTN          Y NG NSSHE+VI+N  G DCHW+EEIID+NLKWSFALN
Sbjct: 1   MGPAVLSSHTN----------YTNGDNSSHESVINNVSGDDCHWYEEIIDENLKWSFALN 60

Query: 61  SVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVF 120
            VLQ+GTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNPK+VF
Sbjct: 61  RVLQQGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNPKSVF 120

Query: 121 IMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARA 180
           IMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRKHLTVN+EAFCHKKLHL+INDAR 
Sbjct: 121 IMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKHLTVNREAFCHKKLHLVINDARV 180

Query: 181 ELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTH 240
           ELE R EK+DVIIGDLADPVKDGPCYKLYTKSFY +IVKPKLH NGIFVTQAGPAGIFTH
Sbjct: 181 ELEKRKEKYDVIIGDLADPVKDGPCYKLYTKSFYHDIVKPKLHHNGIFVTQAGPAGIFTH 240

Query: 241 KEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGE 300
           KEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++RI+GE
Sbjct: 241 KEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDRINGE 300

Query: 301 LLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGV-GFKQLNGGSC 350
           LLYLTG SIVSSTI+NKIVSLALLNETHIYTEATAKFIHG GV G KQLNGGSC
Sbjct: 301 LLYLTGPSIVSSTIINKIVSLALLNETHIYTEATAKFIHGQGVVGCKQLNGGSC 344

[Close]

BLAST of MC08g0476.1 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 506.5 bits (1303), Expect = 1.7e-143
Identity = 239/345 (69.28%), Postives = 281/345 (81.45%), Query Frame = 0


Query: 1   MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL 60
           MG AV     NGF +           S  + + SN  DCHW+EE IDD+LKWSFALNSVL
Sbjct: 1   MGEAVEVMFGNGFPEIH------KATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVL 60

Query: 61  QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG 120
            +GTSE+QDIALLDTKRFGK L+IDGKMQSAE DEF+YHE LIHP LL H NPK VFIMG
Sbjct: 61  HQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMG 120

Query: 121 GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE 180
           GGEGSAARE+LKH + EKVVMCDIDQ+VVDFCR+ LTVN +AFC+KKL L+I DA+AELE
Sbjct: 121 GGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELE 180

Query: 181 TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV 240
            R EKFD+I+GDLADPV+ GPCY+LYTKSFYQ I+KPKL  NGIFVTQAGPAGIFTHKEV
Sbjct: 181 KREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEV 240

Query: 241 FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY 300
           FTSIYNT+KQ FKYV AYTA++PSFADTWGWVMASDH F ++ +EMD+RI+ER++GEL+Y
Sbjct: 241 FTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMY 300

Query: 301 LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQL 346
           L   S VS+  +NK +SLAL  ET +Y+E  A+FIHGHGV ++ +
Sbjct: 301 LNAPSFVSAATLNKTISLALEKETEVYSEENARFIHGHGVAYRHI 339

[Close]

BLAST of MC08g0476.1 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 504.2 bits (1297), Expect = 8.4e-143
Identity = 232/319 (72.73%), Postives = 273/319 (85.58%), Query Frame = 0


Query: 27  SSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVLQKGTSEFQDIALLDTKRFGKALMIDG 86
           S  + + SN  DCHW+EE IDD+LKWSFALNSVL +GTSE+QDIALLDTKRFGK L+IDG
Sbjct: 14  SPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDG 73

Query: 87  KMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQ 146
           KMQSAE DEF+YHE LIHP LL H NPK VFIMGGGEGSAARE+LKH + EKVVMCDIDQ
Sbjct: 74  KMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQ 133

Query: 147 DVVDFCRKHLTVNQEAFCHKKLHLLINDARAELETRNEKFDVIIGDLADPVKDGPCYKLY 206
           +VVDFCR+ LTVN +AFC+KKL L+I DA+AELE R EKFD+I+GDLADPV+ GPCY+LY
Sbjct: 134 EVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLY 193

Query: 207 TKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFA 266
           TKSFYQ I+KPKL  NGIFVTQAGPAGIFTHKEVFTSIYNT+KQ FKYV AYTA++PSFA
Sbjct: 194 TKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFA 253

Query: 267 DTWGWVMASDHPFCIKAEEMDKRIQERIDGELLYLTGASIVSSTIMNKIVSLALLNETHI 326
           DTWGWVMASDH F ++ +EMD+RI+ER++GEL+YL   S VS+  +NK +SLAL  ET +
Sbjct: 254 DTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEV 313

Query: 327 YTEATAKFIHGHGVGFKQL 346
           Y+E  A+FIHGHGV ++ +
Sbjct: 314 YSEENARFIHGHGVAYRHI 332

[Close]

BLAST of MC08g0476.1 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 139.0 bits (349), Expect = 7.1e-33
Identity = 81/259 (31.27%), Postives = 135/259 (52.12%), Query Frame = 0


Query: 24  NGNSSHEAVISNGSDCHWFEEIIDDNLKW-----SFALNSVLQKGTSEFQDIALLDTKRF 83
           NG+   E    +     WF E+   +  W     S  +  VL +G S++QD+ +  +  +
Sbjct: 74  NGDQKKEPACFSTVIPGWFSEM---SPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATY 133

Query: 84  GKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMGGGEGSAAREVLKHKSTEK 143
           GK L++DG +Q  E DE  Y E + H PL    NPK V ++GGG+G   REV +H S E+
Sbjct: 134 GKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQ 193

Query: 144 VVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELETRNE-KFDVIIGDLADPV 203
           + MC+ID+ VVD  ++        +   +++L+I D  A L+   E  +D +I D +DP+
Sbjct: 194 IDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSDPI 253

Query: 204 KDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIA 263
             GP  +L+ K F+Q + +  L   G+  TQA    ++ H ++   I +  ++ FK  + 
Sbjct: 254 --GPAKELFEKPFFQSVAR-ALRPGGVVCTQA--ESLWLHMDIIEDIVSNCREIFKGSVN 313

Query: 264 YT-AYIPSFADTWG-WVMA 275
           Y    +P++   WG W+ A
Sbjct: 314 YAWTSVPTYPRHWGHWIYA 324

[Close]

BLAST of MC08g0476.1 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 134.4 bits (337), Expect = 1.7e-31
Identity = 74/224 (33.04%), Postives = 121/224 (54.02%), Query Frame = 0


Query: 41  WFEEIIDDNLKW-----SFALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADE 100
           WF EI   +  W     S  +  +L +G S++QD+ +  +  +GK L++DG +Q  E DE
Sbjct: 51  WFSEI---SPMWPGEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDE 110

Query: 101 FVYHESLIHPPLLCHSNPKNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKH 160
             Y E + H PL   SNPK V ++GGG+G   REV +H S E++ +C+ID+ VVD  +++
Sbjct: 111 CAYQEMITHLPLCSISNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQY 170

Query: 161 LTVNQEAFCHKKLHLLINDARAELETRNE-KFDVIIGDLADPVKDGPCYKLYTKSFYQEI 220
                  +   +++L+I D  A L+   E  +D +I D +DP+  GP  +L+ K F+ E 
Sbjct: 171 FPNVAVGYEDPRVNLIIGDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFF-ES 230

Query: 221 VKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIAY 259
           V   L   G+  TQA    ++ H ++   I +  +  FK  + Y
Sbjct: 231 VNRALRPGGVVCTQA--ESLWLHMDIIEDIVSNCRDIFKGSVNY 266

[Close]

BLAST of MC08g0476.1 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 132.5 bits (332), Expect = 6.6e-31
Identity = 76/241 (31.54%), Postives = 126/241 (52.28%), Query Frame = 0


Query: 24  NGNSSHEAVISNGSDCHWFEEIIDDNLKW-----SFALNSVLQKGTSEFQDIALLDTKRF 83
           NG+   E    +     WF E+   +  W     S  +  VL +G S++QD+ +  +  +
Sbjct: 74  NGDQKKEPACFSTVIPGWFSEM---SPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATY 133

Query: 84  GKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMGGGEGSAAREVLKHKSTEK 143
           GK L++DG +Q  E DE  Y E + H PL    NPK V ++GGG+G   REV +H S E+
Sbjct: 134 GKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQ 193

Query: 144 VVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELETRNE-KFDVIIGDLADPV 203
           + MC+ID+ VVD  ++        +   +++L+I D  A L+   E  +D +I D +DP+
Sbjct: 194 IDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSDPI 253

Query: 204 KDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIA 259
             GP  +L+ K F+Q + +  L   G+  TQA    ++ H ++   I +  ++ FK  + 
Sbjct: 254 --GPAKELFEKPFFQSVAR-ALRPGGVVCTQA--ESLWLHMDIIEDIVSNCREIFKGSVN 306

The following BLAST results are available for this feature:

BLAST of MC08g0476.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7X6	2.4e-142	69.28	Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV...	[more]
Q975S5	1.7e-55	41.10	Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ...	[more]
Q9UXE4	1.6e-53	41.10	Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 3509...	[more]
Q72K55	1.9e-51	39.24	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / ...	[more]
Q5SK28	1.9e-51	39.24	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DS...	[more]

BLAST of MC08g0476.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131411.1	1.97e-261	100.00	thermospermine synthase ACAULIS5 [Momordica charantia]	[more]
KAG6598599.1	3.59e-234	89.11	Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022961616.1	4.19e-233	88.83	thermospermine synthase ACAULIS5 [Cucurbita moschata]	[more]
XP_022997196.1	8.45e-233	88.27	thermospermine synthase ACAULIS5-like [Cucurbita maxima]	[more]
XP_023546524.1	1.20e-232	88.55	thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC08g0476.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BPF9	9.53e-262	100.00	thermospermine synthase ACAULIS5 OS=Momordica charantia OX=3673 GN=LOC111004633 ...	[more]
A0A6J1HAV2	2.03e-233	88.83	thermospermine synthase ACAULIS5 OS=Cucurbita moschata OX=3662 GN=LOC111462336 P...	[more]
A0A6J1K6U5	4.09e-233	88.27	thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11149216...	[more]
A0A1S3BCH1	2.42e-228	87.74	thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV...	[more]
A0A0A0KIK6	3.48e-222	87.29	PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G362930 PE=3 S...	[more]

BLAST of MC08g0476.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19530.1	1.7e-143	69.28	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G19530.2	8.4e-143	72.73	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G23820.2	7.1e-33	31.27	spermidine synthase 1	[more]
AT1G70310.1	1.7e-31	33.04	spermidine synthase 2	[more]
AT1G23820.1	6.6e-31	31.54	spermidine synthase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037163	Spermidine synthase, tetramerisation domain superfamily	GENE3D	2.30.140.10	Spermidine synthase, tetramerisation domain	coord: 40..91 e-value: 4.8E-14 score: 54.2
IPR035246	Spermidine synthase, tetramerisation domain	PFAM	PF17284	Spermine_synt_N	coord: 41..92 e-value: 1.5E-12 score: 47.2
None	No IPR available	PFAM	PF01564	Spermine_synth	coord: 95..275 e-value: 2.7E-41 score: 141.0
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 92..344 e-value: 9.4E-72 score: 243.5
None	No IPR available	PANTHER	PTHR43317:SF8	BNAA02G04280D PROTEIN	coord: 2..343
None	No IPR available	PANTHER	PTHR43317	THERMOSPERMINE SYNTHASE ACAULIS5	coord: 2..343
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 120..228 e-value: 1.57608E-7 score: 47.0395
IPR001045	Spermidine/spermine synthases	HAMAP	MF_00198	Spermidine_synth	coord: 40..321 score: 29.934734
IPR030374	Polyamine biosynthesis domain	PROSITE	PS51006	PABS_2	coord: 39..276 score: 93.85862
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 40..314

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC08g0476	MC08g0476	gene

The following utr5 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC08g0476.1-utr5	MC08g0476.1-utr5-MC08:3895090..3895281	utr5

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC08g0476.1-cds	MC08g0476.1-cds-MC08:3895282..3895454	CDS
MC08g0476.1-cds	MC08g0476.1-cds-MC08:3895530..3895596	CDS
MC08g0476.1-cds	MC08g0476.1-cds-MC08:3895747..3895841	CDS
MC08g0476.1-cds	MC08g0476.1-cds-MC08:3896447..3896549	CDS
MC08g0476.1-cds	MC08g0476.1-cds-MC08:3896644..3896732	CDS
MC08g0476.1-cds	MC08g0476.1-cds-MC08:3897575..3897731	CDS
MC08g0476.1-cds	MC08g0476.1-cds-MC08:3897815..3897890	CDS
MC08g0476.1-cds	MC08g0476.1-cds-MC08:3897982..3898040	CDS
MC08g0476.1-cds	MC08g0476.1-cds-MC08:3898143..3898279	CDS
MC08g0476.1-cds	MC08g0476.1-cds-MC08:3898442..3898544	CDS

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC08g0476.1-utr3	MC08g0476.1-utr3-MC08:3898545..3899072	utr3

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC08g0476.1	MC08g0476.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006596	polyamine biosynthetic process
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0010487	thermospermine synthase activity