Homology
BLAST of MC08g0476 vs. ExPASy Swiss-Prot
Match:
Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)
HSP 1 Score: 506.5 bits (1303), Expect = 2.4e-142
Identity = 239/345 (69.28%), Postives = 281/345 (81.45%), Query Frame = 0
Query: 1 MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL 60
MG AV NGF + S + + SN DCHW+EE IDD+LKWSFALNSVL
Sbjct: 1 MGEAVEVMFGNGFPEIH------KATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVL 60
Query: 61 QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG 120
+GTSE+QDIALLDTKRFGK L+IDGKMQSAE DEF+YHE LIHP LL H NPK VFIMG
Sbjct: 61 HQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMG 120
Query: 121 GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE 180
GGEGSAARE+LKH + EKVVMCDIDQ+VVDFCR+ LTVN +AFC+KKL L+I DA+AELE
Sbjct: 121 GGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELE 180
Query: 181 TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV 240
R EKFD+I+GDLADPV+ GPCY+LYTKSFYQ I+KPKL NGIFVTQAGPAGIFTHKEV
Sbjct: 181 KREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEV 240
Query: 241 FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY 300
FTSIYNT+KQ FKYV AYTA++PSFADTWGWVMASDH F ++ +EMD+RI+ER++GEL+Y
Sbjct: 241 FTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMY 300
Query: 301 LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQL 346
L S VS+ +NK +SLAL ET +Y+E A+FIHGHGV ++ +
Sbjct: 301 LNAPSFVSAATLNKTISLALEKETEVYSEENARFIHGHGVAYRHI 339
BLAST of MC08g0476 vs. ExPASy Swiss-Prot
Match:
Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)
HSP 1 Score: 218.0 bits (554), Expect = 1.7e-55
Identity = 120/292 (41.10%), Postives = 170/292 (58.22%), Query Frame = 0
Query: 40 HWFEEIIDDNLKWSFALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYH 99
HW E A+ V+ + + +Q L++ RFGKAL++DGK+QS DEF+YH
Sbjct: 5 HWHIEWQTPYEFHGHAITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYH 64
Query: 100 ESLIHPPLLCHSNPKNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTV- 159
E+L+HP LL +NP+ + I+GGGEG+ REVLKHK+ + V M DID V+DF +K+L
Sbjct: 65 EALVHPLLLSINNPEKILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEW 124
Query: 160 NQEAFCHKKLHLLINDARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPK 219
+Q AF + K L+I D ++ EKFD ++ DL DP+KD P LYTK FY+E+ +
Sbjct: 125 HQGAFDNPKSKLVIEDGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KR 184
Query: 220 LHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASD-- 279
+ GI P+ E F+ IYNTIK FK V A Y+P+F WG+V ASD
Sbjct: 185 ISKWGIVTQATSPS---FSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEV 244
Query: 280 HPFCIKAEEMDKRIQERIDGELLYLTGASIVSSTIMNKIVSLALLNETHIYT 329
+P EE++ RI+ERIDG L + G + ++ + K + L E I T
Sbjct: 245 NPAEFSKEEINNRIKERIDGSLRFYDGETHITMFSIPKHIREVLEKENKIST 291
BLAST of MC08g0476 vs. ExPASy Swiss-Prot
Match:
Q9UXE4 (Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=speE PE=1 SV=1)
HSP 1 Score: 211.5 bits (537), Expect = 1.6e-53
Identity = 120/292 (41.10%), Postives = 169/292 (57.88%), Query Frame = 0
Query: 40 HWFEEIIDDNLKWSFALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYH 99
HW E + VL + + +Q + L++ RFGK L+IDGK+QS DE +YH
Sbjct: 5 HWLLEWQTPYEFHGHLIEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYH 64
Query: 100 ESLIHPPLLCHSN-PKNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHL-T 159
E L+HP LL + PK+V I+GGGEG+ REVLK+KS EK VM DID+ V++F +K+L
Sbjct: 65 ELLVHPLLLSLTKPPKSVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYE 124
Query: 160 VNQEAFCHKKLHLLINDARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKP 219
+Q AF K+ +L+I D ++ EK+D II DL DP+K+ Y LYTK FY+++ +
Sbjct: 125 WHQGAFEDKRTNLVITDGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYEKL-RE 184
Query: 220 KLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDH 279
L++ G VTQA EV+ +IYNTIK+ FK A Y+ SF WG+V
Sbjct: 185 ILNERGGIVTQATSPSF--SLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGVR 244
Query: 280 PFCIKAEEMDKRIQERIDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTE 330
P + +E+D +I ERIDG+L + G S S + K + T I TE
Sbjct: 245 PDLLSEDEVDSKINERIDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTE 293
BLAST of MC08g0476 vs. ExPASy Swiss-Prot
Match:
Q72K55 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=speE PE=3 SV=1)
HSP 1 Score: 204.5 bits (519), Expect = 1.9e-51
Identity = 113/288 (39.24%), Postives = 174/288 (60.42%), Query Frame = 0
Query: 56 LNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKN 115
+ V+ G + FQD L ++K FGK L++D +QS E DE++YHE+L+HP +L H PK
Sbjct: 21 MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80
Query: 116 VFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTV-NQEAFCHKKLHLLIND 175
V I+GGGEG+ REVLKH + EK VM DID ++V+ ++H+ +Q AF + L+I+D
Sbjct: 81 VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140
Query: 176 ARAELETRNEKFDVIIGDLADPV-KDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 235
ARA LE E++DV+I DL DPV +D P LYT FY+ +VK L+ G+ QAG
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMI- 200
Query: 236 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASD--HPFCIKAEEMDKRIQ 295
+ TH V ++ T+++ F+YV +Y +IP F +G+++ASD P ++ RI+
Sbjct: 201 LLTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIR 260
Query: 296 ERIDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHG 340
ER + L +LT + + ++ K + AL ET + T+ ++ G
Sbjct: 261 ER-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEG 305
BLAST of MC08g0476 vs. ExPASy Swiss-Prot
Match:
Q5SK28 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=speE PE=1 SV=1)
HSP 1 Score: 204.5 bits (519), Expect = 1.9e-51
Identity = 113/288 (39.24%), Postives = 174/288 (60.42%), Query Frame = 0
Query: 56 LNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKN 115
+ V+ G + FQD L ++K FGK L++D +QS E DE++YHE+L+HP +L H PK
Sbjct: 21 MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80
Query: 116 VFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTV-NQEAFCHKKLHLLIND 175
V I+GGGEG+ REVLKH + EK VM DID ++V+ ++H+ +Q AF + L+I+D
Sbjct: 81 VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140
Query: 176 ARAELETRNEKFDVIIGDLADPV-KDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 235
ARA LE E++DV+I DL DPV +D P LYT FY+ +VK L+ G+ QAG
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYR-LVKAHLNPGGVMGMQAGMI- 200
Query: 236 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASD--HPFCIKAEEMDKRIQ 295
+ TH V ++ T+++ F+YV +Y +IP F +G+++ASD P ++ RI+
Sbjct: 201 LLTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIR 260
Query: 296 ERIDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHG 340
ER + L +LT + + ++ K + AL ET + T+ ++ G
Sbjct: 261 ER-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEG 305
BLAST of MC08g0476 vs. NCBI nr
Match:
XP_022131411.1 (thermospermine synthase ACAULIS5 [Momordica charantia])
HSP 1 Score: 718 bits (1854), Expect = 1.97e-261
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0
Query: 1 MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL 60
MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL
Sbjct: 1 MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL 60
Query: 61 QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG 120
QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG
Sbjct: 61 QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG 120
Query: 121 GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE 180
GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE
Sbjct: 121 GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE 180
Query: 181 TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV 240
TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV
Sbjct: 181 TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV 240
Query: 241 FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY 300
FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY
Sbjct: 241 FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY 300
Query: 301 LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV 352
LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV
Sbjct: 301 LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV 352
BLAST of MC08g0476 vs. NCBI nr
Match:
KAG6598599.1 (Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 650 bits (1676), Expect = 3.59e-234
Identity = 319/358 (89.11%), Postives = 338/358 (94.41%), Query Frame = 0
Query: 1 MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVIS--NGSDCHWFEEIIDDNLKWS 60
MGPAVLSSHTNGFSK F++KSNYING+SSHE+VI+ NG+DC+WFEEIID+NLKWS
Sbjct: 1 MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
Query: 61 FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120
FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
Query: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180
K VFIMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRK+LTVNQE FCHKKLHL+IN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180
Query: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
Query: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300
IFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++R
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300
Query: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351
IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTE TAKFIHG GVGFKQ NGGSCR
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCR 358
BLAST of MC08g0476 vs. NCBI nr
Match:
XP_022961616.1 (thermospermine synthase ACAULIS5 [Cucurbita moschata])
HSP 1 Score: 647 bits (1669), Expect = 4.19e-233
Identity = 318/358 (88.83%), Postives = 338/358 (94.41%), Query Frame = 0
Query: 1 MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVIS--NGSDCHWFEEIIDDNLKWS 60
MGPAVLSSHTNGFSK F++K+NYING+SSHE+VI+ NG+DC+WFEEIID+NLKWS
Sbjct: 1 MGPAVLSSHTNGFSKSVDRNFEEKNNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
Query: 61 FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120
FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
Query: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180
K VFIMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRK+LTVNQE FCHKKLHL+IN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180
Query: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
Query: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300
IFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++R
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300
Query: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351
IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTEATAKFIHG GVGFKQ NG SCR
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTEATAKFIHGQGVGFKQQNGRSCR 358
BLAST of MC08g0476 vs. NCBI nr
Match:
XP_022997196.1 (thermospermine synthase ACAULIS5-like [Cucurbita maxima])
HSP 1 Score: 646 bits (1667), Expect = 8.45e-233
Identity = 316/358 (88.27%), Postives = 338/358 (94.41%), Query Frame = 0
Query: 1 MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVISN--GSDCHWFEEIIDDNLKWS 60
MGPAVLSSHTNGFSK F++KSNYING+SSHE+VI+N G+DC+WFEEIID+NLKWS
Sbjct: 1 MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINNVNGNDCYWFEEIIDENLKWS 60
Query: 61 FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120
FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
Query: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180
K VFIMGGGEGSAAREVLKH+S EKVVMCDIDQDVVDFCRK+LTVNQE FCHKKLHL+IN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHRSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180
Query: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
Query: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300
IFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+E++DK+I++R
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEDLDKKIEDR 300
Query: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351
IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTE TAKFIHG GVGFKQ NGGSCR
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCR 358
BLAST of MC08g0476 vs. NCBI nr
Match:
XP_023546524.1 (thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 646 bits (1666), Expect = 1.20e-232
Identity = 317/358 (88.55%), Postives = 336/358 (93.85%), Query Frame = 0
Query: 1 MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVIS--NGSDCHWFEEIIDDNLKWS 60
MGPAVLSSHTNGFSK F++KSNYING+SSHE+VI+ NG+DC+WFEEIID+NLKWS
Sbjct: 1 MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
Query: 61 FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120
FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
Query: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180
K VFIMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRK+LTVN E FCHKKLHL+IN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNHETFCHKKLHLVIN 180
Query: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
Query: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300
IFTHKEVFTSIYNTIK TF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++R
Sbjct: 241 IFTHKEVFTSIYNTIKHTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300
Query: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351
IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTE TAKFIHG GVGFKQ NGGSCR
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCR 358
BLAST of MC08g0476 vs. ExPASy TrEMBL
Match:
A0A6J1BPF9 (thermospermine synthase ACAULIS5 OS=Momordica charantia OX=3673 GN=LOC111004633 PE=3 SV=1)
HSP 1 Score: 718 bits (1854), Expect = 9.53e-262
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0
Query: 1 MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL 60
MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL
Sbjct: 1 MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL 60
Query: 61 QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG 120
QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG
Sbjct: 61 QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG 120
Query: 121 GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE 180
GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE
Sbjct: 121 GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE 180
Query: 181 TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV 240
TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV
Sbjct: 181 TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV 240
Query: 241 FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY 300
FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY
Sbjct: 241 FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY 300
Query: 301 LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV 352
LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV
Sbjct: 301 LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCRV 352
BLAST of MC08g0476 vs. ExPASy TrEMBL
Match:
A0A6J1HAV2 (thermospermine synthase ACAULIS5 OS=Cucurbita moschata OX=3662 GN=LOC111462336 PE=3 SV=1)
HSP 1 Score: 647 bits (1669), Expect = 2.03e-233
Identity = 318/358 (88.83%), Postives = 338/358 (94.41%), Query Frame = 0
Query: 1 MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVIS--NGSDCHWFEEIIDDNLKWS 60
MGPAVLSSHTNGFSK F++K+NYING+SSHE+VI+ NG+DC+WFEEIID+NLKWS
Sbjct: 1 MGPAVLSSHTNGFSKSVDRNFEEKNNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
Query: 61 FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120
FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
Query: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180
K VFIMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRK+LTVNQE FCHKKLHL+IN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180
Query: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
Query: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300
IFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++R
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDR 300
Query: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351
IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTEATAKFIHG GVGFKQ NG SCR
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTEATAKFIHGQGVGFKQQNGRSCR 358
BLAST of MC08g0476 vs. ExPASy TrEMBL
Match:
A0A6J1K6U5 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111492169 PE=3 SV=1)
HSP 1 Score: 646 bits (1667), Expect = 4.09e-233
Identity = 316/358 (88.27%), Postives = 338/358 (94.41%), Query Frame = 0
Query: 1 MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVISN--GSDCHWFEEIIDDNLKWS 60
MGPAVLSSHTNGFSK F++KSNYING+SSHE+VI+N G+DC+WFEEIID+NLKWS
Sbjct: 1 MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINNVNGNDCYWFEEIIDENLKWS 60
Query: 61 FALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNP 120
FALNSVLQKGTSEFQDIALLDTK FGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNP
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNP 120
Query: 121 KNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLIN 180
K VFIMGGGEGSAAREVLKH+S EKVVMCDIDQDVVDFCRK+LTVNQE FCHKKLHL+IN
Sbjct: 121 KTVFIMGGGEGSAAREVLKHRSIEKVVMCDIDQDVVDFCRKYLTVNQETFCHKKLHLVIN 180
Query: 181 DARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
DA+AELE R EK+D+IIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG
Sbjct: 181 DAKAELEKRKEKYDIIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAG 240
Query: 241 IFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQER 300
IFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+E++DK+I++R
Sbjct: 241 IFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEDLDKKIEDR 300
Query: 301 IDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQLNGGSCR 351
IDGELLYLTG SIVSSTI+NK+VSLALLNETHIYTE TAKFIHG GVGFKQ NGGSCR
Sbjct: 301 IDGELLYLTGPSIVSSTIINKMVSLALLNETHIYTETTAKFIHGQGVGFKQQNGGSCR 358
BLAST of MC08g0476 vs. ExPASy TrEMBL
Match:
A0A1S3BCH1 (thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV=1)
HSP 1 Score: 634 bits (1636), Expect = 2.42e-228
Identity = 315/359 (87.74%), Postives = 336/359 (93.59%), Query Frame = 0
Query: 1 MGPAVLSSHTNGFSK-----FDDKSNYING-NSSHEAVISN--GSDCHWFEEIIDDNLKW 60
MGPAVLSSHTNGFS+ F++K+NYIN NSSHE+VI+N G DCHW+EEIID+NLKW
Sbjct: 1 MGPAVLSSHTNGFSQSVDRNFEEKTNYINDDNSSHESVINNVNGDDCHWYEEIIDENLKW 60
Query: 61 SFALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSN 120
SFALN VLQ+GTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHE LIHPPLLCHSN
Sbjct: 61 SFALNRVLQQGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHECLIHPPLLCHSN 120
Query: 121 PKNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLI 180
PK+VFIMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRKHLTVN+EAFCHKKLHL+I
Sbjct: 121 PKSVFIMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKHLTVNREAFCHKKLHLVI 180
Query: 181 NDARAELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPA 240
NDAR ELE R EK+DVIIGDLADPVKDGPCYKLYTKSFY +I+KPKLH NGIFVTQAGPA
Sbjct: 181 NDARVELEKRKEKYDVIIGDLADPVKDGPCYKLYTKSFYHDILKPKLHHNGIFVTQAGPA 240
Query: 241 GIFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQE 300
GIFTHKEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++
Sbjct: 241 GIFTHKEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIED 300
Query: 301 RIDGELLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGV-GFKQLNGGSC 350
RI+GELLYLTG SIVSSTI+NKIVSLALLNETHIYTEATAKFIHG GV G KQLNGGSC
Sbjct: 301 RINGELLYLTGPSIVSSTIINKIVSLALLNETHIYTEATAKFIHGQGVVGCKQLNGGSC 359
BLAST of MC08g0476 vs. ExPASy TrEMBL
Match:
A0A0A0KIK6 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G362930 PE=3 SV=1)
HSP 1 Score: 618 bits (1594), Expect = 3.48e-222
Identity = 309/354 (87.29%), Postives = 325/354 (91.81%), Query Frame = 0
Query: 1 MGPAVLSSHTNGFSKFDDKSNYING-NSSHEAVISN--GSDCHWFEEIIDDNLKWSFALN 60
MGPAVLSSHTN Y NG NSSHE+VI+N G DCHW+EEIID+NLKWSFALN
Sbjct: 1 MGPAVLSSHTN----------YTNGDNSSHESVINNVSGDDCHWYEEIIDENLKWSFALN 60
Query: 61 SVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVF 120
VLQ+GTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHE LIHP LLCHSNPK+VF
Sbjct: 61 RVLQQGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHECLIHPALLCHSNPKSVF 120
Query: 121 IMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARA 180
IMGGGEGSAAREVLKHKS EKVVMCDIDQDVVDFCRKHLTVN+EAFCHKKLHL+INDAR
Sbjct: 121 IMGGGEGSAAREVLKHKSIEKVVMCDIDQDVVDFCRKHLTVNREAFCHKKLHLVINDARV 180
Query: 181 ELETRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTH 240
ELE R EK+DVIIGDLADPVKDGPCYKLYTKSFY +IVKPKLH NGIFVTQAGPAGIFTH
Sbjct: 181 ELEKRKEKYDVIIGDLADPVKDGPCYKLYTKSFYHDIVKPKLHHNGIFVTQAGPAGIFTH 240
Query: 241 KEVFTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGE 300
KEVFTSIYNTIKQTF YVIAYTAYIPSFADTWGWVMASDHPFCIK+EE+DKRI++RI+GE
Sbjct: 241 KEVFTSIYNTIKQTFNYVIAYTAYIPSFADTWGWVMASDHPFCIKSEELDKRIEDRINGE 300
Query: 301 LLYLTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGV-GFKQLNGGSC 350
LLYLTG SIVSSTI+NKIVSLALLNETHIYTEATAKFIHG GV G KQLNGGSC
Sbjct: 301 LLYLTGPSIVSSTIINKIVSLALLNETHIYTEATAKFIHGQGVVGCKQLNGGSC 344
BLAST of MC08g0476 vs. TAIR 10
Match:
AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )
HSP 1 Score: 506.5 bits (1303), Expect = 1.7e-143
Identity = 239/345 (69.28%), Postives = 281/345 (81.45%), Query Frame = 0
Query: 1 MGPAVLSSHTNGFSKFDDKSNYINGNSSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVL 60
MG AV NGF + S + + SN DCHW+EE IDD+LKWSFALNSVL
Sbjct: 1 MGEAVEVMFGNGFPEIH------KATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVL 60
Query: 61 QKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMG 120
+GTSE+QDIALLDTKRFGK L+IDGKMQSAE DEF+YHE LIHP LL H NPK VFIMG
Sbjct: 61 HQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMG 120
Query: 121 GGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELE 180
GGEGSAARE+LKH + EKVVMCDIDQ+VVDFCR+ LTVN +AFC+KKL L+I DA+AELE
Sbjct: 121 GGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELE 180
Query: 181 TRNEKFDVIIGDLADPVKDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEV 240
R EKFD+I+GDLADPV+ GPCY+LYTKSFYQ I+KPKL NGIFVTQAGPAGIFTHKEV
Sbjct: 181 KREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEV 240
Query: 241 FTSIYNTIKQTFKYVIAYTAYIPSFADTWGWVMASDHPFCIKAEEMDKRIQERIDGELLY 300
FTSIYNT+KQ FKYV AYTA++PSFADTWGWVMASDH F ++ +EMD+RI+ER++GEL+Y
Sbjct: 241 FTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMY 300
Query: 301 LTGASIVSSTIMNKIVSLALLNETHIYTEATAKFIHGHGVGFKQL 346
L S VS+ +NK +SLAL ET +Y+E A+FIHGHGV ++ +
Sbjct: 301 LNAPSFVSAATLNKTISLALEKETEVYSEENARFIHGHGVAYRHI 339
BLAST of MC08g0476 vs. TAIR 10
Match:
AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )
HSP 1 Score: 504.2 bits (1297), Expect = 8.4e-143
Identity = 232/319 (72.73%), Postives = 273/319 (85.58%), Query Frame = 0
Query: 27 SSHEAVISNGSDCHWFEEIIDDNLKWSFALNSVLQKGTSEFQDIALLDTKRFGKALMIDG 86
S + + SN DCHW+EE IDD+LKWSFALNSVL +GTSE+QDIALLDTKRFGK L+IDG
Sbjct: 14 SPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDG 73
Query: 87 KMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQ 146
KMQSAE DEF+YHE LIHP LL H NPK VFIMGGGEGSAARE+LKH + EKVVMCDIDQ
Sbjct: 74 KMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQ 133
Query: 147 DVVDFCRKHLTVNQEAFCHKKLHLLINDARAELETRNEKFDVIIGDLADPVKDGPCYKLY 206
+VVDFCR+ LTVN +AFC+KKL L+I DA+AELE R EKFD+I+GDLADPV+ GPCY+LY
Sbjct: 134 EVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLY 193
Query: 207 TKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIAYTAYIPSFA 266
TKSFYQ I+KPKL NGIFVTQAGPAGIFTHKEVFTSIYNT+KQ FKYV AYTA++PSFA
Sbjct: 194 TKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFA 253
Query: 267 DTWGWVMASDHPFCIKAEEMDKRIQERIDGELLYLTGASIVSSTIMNKIVSLALLNETHI 326
DTWGWVMASDH F ++ +EMD+RI+ER++GEL+YL S VS+ +NK +SLAL ET +
Sbjct: 254 DTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEV 313
Query: 327 YTEATAKFIHGHGVGFKQL 346
Y+E A+FIHGHGV ++ +
Sbjct: 314 YSEENARFIHGHGVAYRHI 332
BLAST of MC08g0476 vs. TAIR 10
Match:
AT1G23820.2 (spermidine synthase 1 )
HSP 1 Score: 139.0 bits (349), Expect = 7.1e-33
Identity = 81/259 (31.27%), Postives = 135/259 (52.12%), Query Frame = 0
Query: 24 NGNSSHEAVISNGSDCHWFEEIIDDNLKW-----SFALNSVLQKGTSEFQDIALLDTKRF 83
NG+ E + WF E+ + W S + VL +G S++QD+ + + +
Sbjct: 74 NGDQKKEPACFSTVIPGWFSEM---SPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATY 133
Query: 84 GKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMGGGEGSAAREVLKHKSTEK 143
GK L++DG +Q E DE Y E + H PL NPK V ++GGG+G REV +H S E+
Sbjct: 134 GKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQ 193
Query: 144 VVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELETRNE-KFDVIIGDLADPV 203
+ MC+ID+ VVD ++ + +++L+I D A L+ E +D +I D +DP+
Sbjct: 194 IDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSDPI 253
Query: 204 KDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIA 263
GP +L+ K F+Q + + L G+ TQA ++ H ++ I + ++ FK +
Sbjct: 254 --GPAKELFEKPFFQSVAR-ALRPGGVVCTQA--ESLWLHMDIIEDIVSNCREIFKGSVN 313
Query: 264 YT-AYIPSFADTWG-WVMA 275
Y +P++ WG W+ A
Sbjct: 314 YAWTSVPTYPRHWGHWIYA 324
BLAST of MC08g0476 vs. TAIR 10
Match:
AT1G70310.1 (spermidine synthase 2 )
HSP 1 Score: 134.4 bits (337), Expect = 1.7e-31
Identity = 74/224 (33.04%), Postives = 121/224 (54.02%), Query Frame = 0
Query: 41 WFEEIIDDNLKW-----SFALNSVLQKGTSEFQDIALLDTKRFGKALMIDGKMQSAEADE 100
WF EI + W S + +L +G S++QD+ + + +GK L++DG +Q E DE
Sbjct: 51 WFSEI---SPMWPGEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDE 110
Query: 101 FVYHESLIHPPLLCHSNPKNVFIMGGGEGSAAREVLKHKSTEKVVMCDIDQDVVDFCRKH 160
Y E + H PL SNPK V ++GGG+G REV +H S E++ +C+ID+ VVD +++
Sbjct: 111 CAYQEMITHLPLCSISNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQY 170
Query: 161 LTVNQEAFCHKKLHLLINDARAELETRNE-KFDVIIGDLADPVKDGPCYKLYTKSFYQEI 220
+ +++L+I D A L+ E +D +I D +DP+ GP +L+ K F+ E
Sbjct: 171 FPNVAVGYEDPRVNLIIGDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFF-ES 230
Query: 221 VKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIAY 259
V L G+ TQA ++ H ++ I + + FK + Y
Sbjct: 231 VNRALRPGGVVCTQA--ESLWLHMDIIEDIVSNCRDIFKGSVNY 266
BLAST of MC08g0476 vs. TAIR 10
Match:
AT1G23820.1 (spermidine synthase 1 )
HSP 1 Score: 132.5 bits (332), Expect = 6.6e-31
Identity = 76/241 (31.54%), Postives = 126/241 (52.28%), Query Frame = 0
Query: 24 NGNSSHEAVISNGSDCHWFEEIIDDNLKW-----SFALNSVLQKGTSEFQDIALLDTKRF 83
NG+ E + WF E+ + W S + VL +G S++QD+ + + +
Sbjct: 74 NGDQKKEPACFSTVIPGWFSEM---SPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATY 133
Query: 84 GKALMIDGKMQSAEADEFVYHESLIHPPLLCHSNPKNVFIMGGGEGSAAREVLKHKSTEK 143
GK L++DG +Q E DE Y E + H PL NPK V ++GGG+G REV +H S E+
Sbjct: 134 GKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQ 193
Query: 144 VVMCDIDQDVVDFCRKHLTVNQEAFCHKKLHLLINDARAELETRNE-KFDVIIGDLADPV 203
+ MC+ID+ VVD ++ + +++L+I D A L+ E +D +I D +DP+
Sbjct: 194 IDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSDPI 253
Query: 204 KDGPCYKLYTKSFYQEIVKPKLHDNGIFVTQAGPAGIFTHKEVFTSIYNTIKQTFKYVIA 259
GP +L+ K F+Q + + L G+ TQA ++ H ++ I + ++ FK +
Sbjct: 254 --GPAKELFEKPFFQSVAR-ALRPGGVVCTQA--ESLWLHMDIIEDIVSNCREIFKGSVN 306
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9S7X6 | 2.4e-142 | 69.28 | Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV... | [more] |
Q975S5 | 1.7e-55 | 41.10 | Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ... | [more] |
Q9UXE4 | 1.6e-53 | 41.10 | Polyamine aminopropyltransferase OS=Saccharolobus solfataricus (strain ATCC 3509... | [more] |
Q72K55 | 1.9e-51 | 39.24 | Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / ... | [more] |
Q5SK28 | 1.9e-51 | 39.24 | Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DS... | [more] |
Match Name | E-value | Identity | Description | |
XP_022131411.1 | 1.97e-261 | 100.00 | thermospermine synthase ACAULIS5 [Momordica charantia] | [more] |
KAG6598599.1 | 3.59e-234 | 89.11 | Thermospermine synthase ACAULIS5, partial [Cucurbita argyrosperma subsp. sororia... | [more] |
XP_022961616.1 | 4.19e-233 | 88.83 | thermospermine synthase ACAULIS5 [Cucurbita moschata] | [more] |
XP_022997196.1 | 8.45e-233 | 88.27 | thermospermine synthase ACAULIS5-like [Cucurbita maxima] | [more] |
XP_023546524.1 | 1.20e-232 | 88.55 | thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1BPF9 | 9.53e-262 | 100.00 | thermospermine synthase ACAULIS5 OS=Momordica charantia OX=3673 GN=LOC111004633 ... | [more] |
A0A6J1HAV2 | 2.03e-233 | 88.83 | thermospermine synthase ACAULIS5 OS=Cucurbita moschata OX=3662 GN=LOC111462336 P... | [more] |
A0A6J1K6U5 | 4.09e-233 | 88.27 | thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11149216... | [more] |
A0A1S3BCH1 | 2.42e-228 | 87.74 | thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV... | [more] |
A0A0A0KIK6 | 3.48e-222 | 87.29 | PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G362930 PE=3 S... | [more] |