MC05g1244.1 (mRNA) Bitter gourd (Dali-11) v1

Overview
NameMC05g1244.1
TypemRNA
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionUbiquitin-conjugating enzyme E2 variant 1D
LocationMC05: 16675316 .. 16676278 (-)
Sequence length405
RNA-Seq ExpressionMC05g1244.1
SyntenyMC05g1244.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTCCTAGGAACTTCAGGTTGCTGGAGGAACTTGAACGTGGAGAAAAGGGAATTGGCGATGGCACTGTGAGCTATGGAATGGATGATGGAGATGACATTTATATGCGTTCATGGACTGGCACCATCATTGGCCCACACAATGTAATGGCTTCTGCTCTTCTCTTCAGGAGTTTTAATCGCTTGCTCATGAAGAAAATTTGAAAATTTACACTACATTAACTACCACTCAAATTAAACTTGCTCTTTTCCGTTTGTAATTAGCATAATATTTTTTTTGCCTTGAAGTCTGGCGAAATGCTCTTACTTTTAAATGGTTGATAAAGAGGTGCTGTTAGGGAATCTTATGCTAAAAATTGGAAATGTCTACTTTATTTTACAATTCACAATTCCTATTATAAGGCGACACCTACAGTGCATTAAAAATTCATAATGCTTCTTTTTTCCTTGCAGACGGTACATGAAGGTAGAATTTATCAGTTGAAGCTGTTCTGTGATAAAGATTACCCTGATAAGCCACCAAGTGTTCGTTTTCATTCCCGAGTCAACATGACATGCGTGAACCATGAAACTGGAGTGGTATCTCTGATTTCACTCCCTCAGTTGATAATATGCATATCTTAGTACTTTATGTTGAACTAAAAGTTGACGTTTATAAGAAGACCAAAAGTTTTCATTTACCTGCAAATACATAGATTACCTCTGACAGCAGCCCACCGTCATGGATATCATTTACCTACAACTACATAGATGATTTTATCAACCAATCTTGCTCTTCTTCTCTTTGAAACTGATGAATTTCTACGTTATTAATTGATTTCCCAACAGGTCGAGTCGAAGAAATTTGGACTTCTTGCAAATTGGCAGCGAGAGTACACCATGGAGGATATACTCACACAGCTGAAAAAGGAAATGGCTGCCCCACATAACCGAAAGCTCGTCCAACCTCCAGAGGGCACCTACTTC

mRNA sequence

GTTCCTAGGAACTTCAGGTTGCTGGAGGAACTTGAACGTGGAGAAAAGGGAATTGGCGATGGCACTGTGAGCTATGGAATGGATGATGGAGATGACATTTATATGCGTTCATGGACTGGCACCATCATTGGCCCACACAATACGGTACATGAAGGTAGAATTTATCAGTTGAAGCTGTTCTGTGATAAAGATTACCCTGATAAGCCACCAAGTGTTCGTTTTCATTCCCGAGTCAACATGACATGCGTGAACCATGAAACTGGAGTGGTCGAGTCGAAGAAATTTGGACTTCTTGCAAATTGGCAGCGAGAGTACACCATGGAGGATATACTCACACAGCTGAAAAAGGAAATGGCTGCCCCACATAACCGAAAGCTCGTCCAACCTCCAGAGGGCACCTACTTC

Coding sequence (CDS)

GTTCCTAGGAACTTCAGGTTGCTGGAGGAACTTGAACGTGGAGAAAAGGGAATTGGCGATGGCACTGTGAGCTATGGAATGGATGATGGAGATGACATTTATATGCGTTCATGGACTGGCACCATCATTGGCCCACACAATACGGTACATGAAGGTAGAATTTATCAGTTGAAGCTGTTCTGTGATAAAGATTACCCTGATAAGCCACCAAGTGTTCGTTTTCATTCCCGAGTCAACATGACATGCGTGAACCATGAAACTGGAGTGGTCGAGTCGAAGAAATTTGGACTTCTTGCAAATTGGCAGCGAGAGTACACCATGGAGGATATACTCACACAGCTGAAAAAGGAAATGGCTGCCCCACATAACCGAAAGCTCGTCCAACCTCCAGAGGGCACCTACTTC

Protein sequence

VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLFCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAAPHNRKLVQPPEGTYF
Homology
BLAST of MC05g1244.1 vs. ExPASy Swiss-Prot
Match: Q9SJ44 (Ubiquitin-conjugating enzyme E2 variant 1C OS=Arabidopsis thaliana OX=3702 GN=UEV1C PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 4.8e-75
Identity = 126/135 (93.33%), Postives = 134/135 (99.26%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 11  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 70

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYP+KPP+VRFHSR+NMTCVNH+TGVV+SKKFG+LANWQR+YTMEDILTQLKKEMAA
Sbjct: 71  CDKDYPEKPPTVRFHSRINMTCVNHDTGVVDSKKFGVLANWQRQYTMEDILTQLKKEMAA 130

Query: 121 PHNRKLVQPPEGTYF 136
            HNRKLVQPPEGT+F
Sbjct: 131 SHNRKLVQPPEGTFF 145

BLAST of MC05g1244.1 vs. ExPASy Swiss-Prot
Match: Q9SVD7 (Ubiquitin-conjugating enzyme E2 variant 1D OS=Arabidopsis thaliana OX=3702 GN=UEV1D PE=1 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 4.5e-73
Identity = 125/135 (92.59%), Postives = 129/135 (95.56%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 71

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYP+KPP+VRFHSRVNM CVNHETGVV+ KKFGLLANWQREYTMEDIL QLKKEM+ 
Sbjct: 72  CDKDYPEKPPTVRFHSRVNMACVNHETGVVDPKKFGLLANWQREYTMEDILVQLKKEMST 131

Query: 121 PHNRKLVQPPEGTYF 136
            HNRKLVQPPEGT F
Sbjct: 132 SHNRKLVQPPEGTCF 146

BLAST of MC05g1244.1 vs. ExPASy Swiss-Prot
Match: Q93YP0 (Ubiquitin-conjugating enzyme E2 variant 1A OS=Arabidopsis thaliana OX=3702 GN=UEV1A PE=1 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 1.1e-58
Identity = 101/132 (76.52%), Postives = 114/132 (86.36%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDD DDIYM+SWTGTI+GP NT +EG+I+QLKLF
Sbjct: 10  VPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMQSWTGTILGPPNTAYEGKIFQLKLF 69

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           C K+YP+ PP+VRF +R+NM CVN ETGVVE   F +L NW+REYTMEDIL +LKKEM  
Sbjct: 70  CGKEYPESPPTVRFQTRINMACVNPETGVVEPSLFPMLTNWRREYTMEDILVKLKKEMMT 129

Query: 121 PHNRKLVQPPEG 133
            HNRKL QPPEG
Sbjct: 130 SHNRKLAQPPEG 141

BLAST of MC05g1244.1 vs. ExPASy Swiss-Prot
Match: Q9CAB6 (Ubiquitin-conjugating enzyme E2 variant 1B OS=Arabidopsis thaliana OX=3702 GN=UEV1B PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 1.0e-56
Identity = 98/132 (74.24%), Postives = 113/132 (85.61%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDD DDI M+SWTGTI+GPHNT +EG+I+QLKLF
Sbjct: 10  VPRNFRLLEELERGEKGIGDGTVSYGMDDADDILMQSWTGTILGPHNTAYEGKIFQLKLF 69

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           C KDYP+ PP+VRF SR+NM CVN E GVV+   F +L+NW+RE+TMED+L QLKKEM +
Sbjct: 70  CGKDYPESPPTVRFQSRINMACVNPENGVVDPSHFPMLSNWRREFTMEDLLIQLKKEMMS 129

Query: 121 PHNRKLVQPPEG 133
             NRKL QP EG
Sbjct: 130 SQNRKLAQPLEG 141

BLAST of MC05g1244.1 vs. ExPASy Swiss-Prot
Match: Q54D06 (Probable ubiquitin-conjugating enzyme E2 variant OS=Dictyostelium discoideum OX=44689 GN=ube2v PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 1.5e-44
Identity = 76/135 (56.30%), Postives = 104/135 (77.04%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           +PRNF LLEELE GEK  GDGT+SYG++  DDI + SW GTIIGP NT  + R Y LKL+
Sbjct: 4   IPRNFVLLEELESGEKSTGDGTISYGLESPDDILLSSWIGTIIGPQNTPFDNRFYSLKLY 63

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYP+KPP+VRF +++N+ CVN + GVV+ K   +L+NW  +  +++IL++L++EM++
Sbjct: 64  CDKDYPNKPPTVRFTTKINIGCVNQQNGVVDPKNLPILSNWTSKNRIQNILSELRREMSS 123

Query: 121 PHNRKLVQPPEGTYF 136
             N+KLVQPPEG  F
Sbjct: 124 SSNKKLVQPPEGANF 138

BLAST of MC05g1244.1 vs. NCBI nr
Match: XP_022144653.1 (ubiquitin-conjugating enzyme E2 variant 1C [Momordica charantia] >XP_038904037.1 ubiquitin-conjugating enzyme E2 variant 1C [Benincasa hispida])

HSP 1 Score: 292 bits (747), Expect = 9.68e-100
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 71

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA
Sbjct: 72  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 131

Query: 121 PHNRKLVQPPEGTYF 135
           PHNRKLVQPPEGTYF
Sbjct: 132 PHNRKLVQPPEGTYF 146

BLAST of MC05g1244.1 vs. NCBI nr
Match: XP_004136503.1 (ubiquitin-conjugating enzyme E2 variant 1C [Cucumis sativus] >XP_008442892.1 PREDICTED: ubiquitin-conjugating enzyme E2 variant 1C [Cucumis melo] >KGN59179.1 hypothetical protein Csa_001903 [Cucumis sativus])

HSP 1 Score: 290 bits (743), Expect = 3.94e-99
Identity = 134/135 (99.26%), Postives = 135/135 (100.00%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 71

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYP+KPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA
Sbjct: 72  CDKDYPEKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 131

Query: 121 PHNRKLVQPPEGTYF 135
           PHNRKLVQPPEGTYF
Sbjct: 132 PHNRKLVQPPEGTYF 146

BLAST of MC05g1244.1 vs. NCBI nr
Match: XP_022983154.1 (ubiquitin-conjugating enzyme E2 variant 1C [Cucurbita maxima])

HSP 1 Score: 290 bits (743), Expect = 3.94e-99
Identity = 134/135 (99.26%), Postives = 135/135 (100.00%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 71

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREY+MEDILTQLKKEMAA
Sbjct: 72  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYSMEDILTQLKKEMAA 131

Query: 121 PHNRKLVQPPEGTYF 135
           PHNRKLVQPPEGTYF
Sbjct: 132 PHNRKLVQPPEGTYF 146

BLAST of MC05g1244.1 vs. NCBI nr
Match: XP_021636663.1 (ubiquitin-conjugating enzyme E2 variant 1C isoform X2 [Hevea brasiliensis] >XP_022766996.1 ubiquitin-conjugating enzyme E2 variant 1C [Durio zibethinus])

HSP 1 Score: 290 bits (742), Expect = 5.60e-99
Identity = 133/135 (98.52%), Postives = 135/135 (100.00%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 71

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYP+KPPSVRFHSR+NMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA
Sbjct: 72  CDKDYPEKPPSVRFHSRINMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 131

Query: 121 PHNRKLVQPPEGTYF 135
           PHNRKLVQPPEGTYF
Sbjct: 132 PHNRKLVQPPEGTYF 146

BLAST of MC05g1244.1 vs. NCBI nr
Match: XP_030543544.1 (ubiquitin-conjugating enzyme E2 variant 1C [Rhodamnia argentea] >XP_030543555.1 ubiquitin-conjugating enzyme E2 variant 1C [Rhodamnia argentea] >XP_030543563.1 ubiquitin-conjugating enzyme E2 variant 1C [Rhodamnia argentea])

HSP 1 Score: 290 bits (742), Expect = 5.60e-99
Identity = 133/135 (98.52%), Postives = 135/135 (100.00%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 71

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYP+KPPSVRFHSR+NMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA
Sbjct: 72  CDKDYPEKPPSVRFHSRINMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 131

Query: 121 PHNRKLVQPPEGTYF 135
           PHNRKLVQPPEGTYF
Sbjct: 132 PHNRKLVQPPEGTYF 146

BLAST of MC05g1244.1 vs. ExPASy TrEMBL
Match: A0A6J1CTV6 (ubiquitin-conjugating enzyme E2 variant 1C OS=Momordica charantia OX=3673 GN=LOC111014288 PE=4 SV=1)

HSP 1 Score: 292 bits (747), Expect = 4.68e-100
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 71

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA
Sbjct: 72  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 131

Query: 121 PHNRKLVQPPEGTYF 135
           PHNRKLVQPPEGTYF
Sbjct: 132 PHNRKLVQPPEGTYF 146

BLAST of MC05g1244.1 vs. ExPASy TrEMBL
Match: A0A6J1J6I4 (ubiquitin-conjugating enzyme E2 variant 1C OS=Cucurbita maxima OX=3661 GN=LOC111481792 PE=4 SV=1)

HSP 1 Score: 290 bits (743), Expect = 1.91e-99
Identity = 134/135 (99.26%), Postives = 135/135 (100.00%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 71

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREY+MEDILTQLKKEMAA
Sbjct: 72  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYSMEDILTQLKKEMAA 131

Query: 121 PHNRKLVQPPEGTYF 135
           PHNRKLVQPPEGTYF
Sbjct: 132 PHNRKLVQPPEGTYF 146

BLAST of MC05g1244.1 vs. ExPASy TrEMBL
Match: A0A0A0LE42 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G778350 PE=4 SV=1)

HSP 1 Score: 290 bits (743), Expect = 1.91e-99
Identity = 134/135 (99.26%), Postives = 135/135 (100.00%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 71

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYP+KPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA
Sbjct: 72  CDKDYPEKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 131

Query: 121 PHNRKLVQPPEGTYF 135
           PHNRKLVQPPEGTYF
Sbjct: 132 PHNRKLVQPPEGTYF 146

BLAST of MC05g1244.1 vs. ExPASy TrEMBL
Match: A0A1S3B6A6 (ubiquitin-conjugating enzyme E2 variant 1C OS=Cucumis melo OX=3656 GN=LOC103486656 PE=4 SV=1)

HSP 1 Score: 290 bits (743), Expect = 1.91e-99
Identity = 134/135 (99.26%), Postives = 135/135 (100.00%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 71

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYP+KPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA
Sbjct: 72  CDKDYPEKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 131

Query: 121 PHNRKLVQPPEGTYF 135
           PHNRKLVQPPEGTYF
Sbjct: 132 PHNRKLVQPPEGTYF 146

BLAST of MC05g1244.1 vs. ExPASy TrEMBL
Match: A0A6P6AQA1 (ubiquitin-conjugating enzyme E2 variant 1C OS=Durio zibethinus OX=66656 GN=LOC111311670 PE=4 SV=1)

HSP 1 Score: 290 bits (742), Expect = 2.71e-99
Identity = 133/135 (98.52%), Postives = 135/135 (100.00%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 71

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYP+KPPSVRFHSR+NMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA
Sbjct: 72  CDKDYPEKPPSVRFHSRINMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 131

Query: 121 PHNRKLVQPPEGTYF 135
           PHNRKLVQPPEGTYF
Sbjct: 132 PHNRKLVQPPEGTYF 146

BLAST of MC05g1244.1 vs. TAIR 10
Match: AT2G36060.1 (MMS ZWEI homologue 3 )

HSP 1 Score: 281.6 bits (719), Expect = 3.4e-76
Identity = 126/135 (93.33%), Postives = 134/135 (99.26%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 11  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 70

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYP+KPP+VRFHSR+NMTCVNH+TGVV+SKKFG+LANWQR+YTMEDILTQLKKEMAA
Sbjct: 71  CDKDYPEKPPTVRFHSRINMTCVNHDTGVVDSKKFGVLANWQRQYTMEDILTQLKKEMAA 130

Query: 121 PHNRKLVQPPEGTYF 136
            HNRKLVQPPEGT+F
Sbjct: 131 SHNRKLVQPPEGTFF 145

BLAST of MC05g1244.1 vs. TAIR 10
Match: AT2G36060.2 (MMS ZWEI homologue 3 )

HSP 1 Score: 276.9 bits (707), Expect = 8.4e-75
Identity = 126/136 (92.65%), Postives = 134/136 (98.53%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHN-TVHEGRIYQLKL 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHN TVHEGRIYQLKL
Sbjct: 11  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNVTVHEGRIYQLKL 70

Query: 61  FCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMA 120
           FCDKDYP+KPP+VRFHSR+NMTCVNH+TGVV+SKKFG+LANWQR+YTMEDILTQLKKEMA
Sbjct: 71  FCDKDYPEKPPTVRFHSRINMTCVNHDTGVVDSKKFGVLANWQRQYTMEDILTQLKKEMA 130

Query: 121 APHNRKLVQPPEGTYF 136
           A HNRKLVQPPEGT+F
Sbjct: 131 ASHNRKLVQPPEGTFF 146

BLAST of MC05g1244.1 vs. TAIR 10
Match: AT2G36060.3 (MMS ZWEI homologue 3 )

HSP 1 Score: 275.4 bits (703), Expect = 2.4e-74
Identity = 125/135 (92.59%), Postives = 133/135 (98.52%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 11  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 70

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYP+KPP+VRFHSR+NMTCVNH+TG V+SKKFG+LANWQR+YTMEDILTQLKKEMAA
Sbjct: 71  CDKDYPEKPPTVRFHSRINMTCVNHDTG-VDSKKFGVLANWQRQYTMEDILTQLKKEMAA 130

Query: 121 PHNRKLVQPPEGTYF 136
            HNRKLVQPPEGT+F
Sbjct: 131 SHNRKLVQPPEGTFF 144

BLAST of MC05g1244.1 vs. TAIR 10
Match: AT3G52560.1 (ubiquitin E2 variant 1D-4 )

HSP 1 Score: 275.0 bits (702), Expect = 3.2e-74
Identity = 125/135 (92.59%), Postives = 129/135 (95.56%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLF 71

Query: 61  CDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMAA 120
           CDKDYP+KPP+VRFHSRVNM CVNHETGVV+ KKFGLLANWQREYTMEDIL QLKKEM+ 
Sbjct: 72  CDKDYPEKPPTVRFHSRVNMACVNHETGVVDPKKFGLLANWQREYTMEDILVQLKKEMST 131

Query: 121 PHNRKLVQPPEGTYF 136
            HNRKLVQPPEGT F
Sbjct: 132 SHNRKLVQPPEGTCF 146

BLAST of MC05g1244.1 vs. TAIR 10
Match: AT3G52560.2 (ubiquitin E2 variant 1D-4 )

HSP 1 Score: 270.4 bits (690), Expect = 7.8e-73
Identity = 125/136 (91.91%), Postives = 129/136 (94.85%), Query Frame = 0

Query: 1   VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHN-TVHEGRIYQLKL 60
           VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHN TVHEGRIYQLKL
Sbjct: 12  VPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNVTVHEGRIYQLKL 71

Query: 61  FCDKDYPDKPPSVRFHSRVNMTCVNHETGVVESKKFGLLANWQREYTMEDILTQLKKEMA 120
           FCDKDYP+KPP+VRFHSRVNM CVNHETGVV+ KKFGLLANWQREYTMEDIL QLKKEM+
Sbjct: 72  FCDKDYPEKPPTVRFHSRVNMACVNHETGVVDPKKFGLLANWQREYTMEDILVQLKKEMS 131

Query: 121 APHNRKLVQPPEGTYF 136
             HNRKLVQPPEGT F
Sbjct: 132 TSHNRKLVQPPEGTCF 147

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SJ444.8e-7593.33Ubiquitin-conjugating enzyme E2 variant 1C OS=Arabidopsis thaliana OX=3702 GN=UE... [more]
Q9SVD74.5e-7392.59Ubiquitin-conjugating enzyme E2 variant 1D OS=Arabidopsis thaliana OX=3702 GN=UE... [more]
Q93YP01.1e-5876.52Ubiquitin-conjugating enzyme E2 variant 1A OS=Arabidopsis thaliana OX=3702 GN=UE... [more]
Q9CAB61.0e-5674.24Ubiquitin-conjugating enzyme E2 variant 1B OS=Arabidopsis thaliana OX=3702 GN=UE... [more]
Q54D061.5e-4456.30Probable ubiquitin-conjugating enzyme E2 variant OS=Dictyostelium discoideum OX=... [more]
Match NameE-valueIdentityDescription
XP_022144653.19.68e-100100.00ubiquitin-conjugating enzyme E2 variant 1C [Momordica charantia] >XP_038904037.1... [more]
XP_004136503.13.94e-9999.26ubiquitin-conjugating enzyme E2 variant 1C [Cucumis sativus] >XP_008442892.1 PRE... [more]
XP_022983154.13.94e-9999.26ubiquitin-conjugating enzyme E2 variant 1C [Cucurbita maxima][more]
XP_021636663.15.60e-9998.52ubiquitin-conjugating enzyme E2 variant 1C isoform X2 [Hevea brasiliensis] >XP_0... [more]
XP_030543544.15.60e-9998.52ubiquitin-conjugating enzyme E2 variant 1C [Rhodamnia argentea] >XP_030543555.1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CTV64.68e-100100.00ubiquitin-conjugating enzyme E2 variant 1C OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1J6I41.91e-9999.26ubiquitin-conjugating enzyme E2 variant 1C OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0LE421.91e-9999.26UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G778350 PE... [more]
A0A1S3B6A61.91e-9999.26ubiquitin-conjugating enzyme E2 variant 1C OS=Cucumis melo OX=3656 GN=LOC1034866... [more]
A0A6P6AQA12.71e-9998.52ubiquitin-conjugating enzyme E2 variant 1C OS=Durio zibethinus OX=66656 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT2G36060.13.4e-7693.33MMS ZWEI homologue 3 [more]
AT2G36060.28.4e-7592.65MMS ZWEI homologue 3 [more]
AT2G36060.32.4e-7492.59MMS ZWEI homologue 3 [more]
AT3G52560.13.2e-7492.59ubiquitin E2 variant 1D-4 [more]
AT3G52560.27.8e-7391.91ubiquitin E2 variant 1D-4 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 5..133
e-value: 9.3E-5
score: 31.2
NoneNo IPR availablePANTHERPTHR24068:SF318UBIQUITIN-CONJUGATING ENZYME/RWD-LIKE PROTEIN-RELATEDcoord: 3..129
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 3..129
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 32..125
e-value: 1.5E-14
score: 53.8
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 2..135
score: 31.934349
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 1..135
e-value: 3.6E-42
score: 145.4
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 2..135

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MC05g1244MC05g1244gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC05g1244.1-cdsMC05g1244.1-cds-MC05:16675316..16675453CDS
MC05g1244.1-cdsMC05g1244.1-cds-MC05:16675703..16675828CDS
MC05g1244.1-cdsMC05g1244.1-cds-MC05:16676138..16676278CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MC05g1244.1MC05g1244.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006301 postreplication repair
biological_process GO:0070534 protein K63-linked ubiquitination
cellular_component GO:0005634 nucleus