MC05g0802.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC05g0802.1 vs. ExPASy Swiss-Prot
Match: B6TTV8 (Protein WHAT'S THIS FACTOR 1, chloroplastic OS=Zea mays OX=4577 GN=WTF1 PE=1 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.2e-56
Identity = 152/411 (36.98%), Postives = 215/411 (52.31%), Query Frame = 0

Query: 70  KHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYPSIFELFSIP 129
           +  K + V KL  +L+++P   + L  L + R  L L + R L++++ R+P +F++    
Sbjct: 69  RDKKLKLVLKLRNILVAQPDRVMSLRELGRFRRDLGLTRKRRLIALLRRFPGVFDVVE-- 128

Query: 130 YPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLMLSSHHRILLS 189
                     +Y  L  RLTPAA  L   +  L+         KL+KLLM+S   RIL+ 
Sbjct: 129 --------EGVY-SLRFRLTPAAERLYLDELRLRNESEGLAVAKLRKLLMMSQEKRILIE 188

Query: 190 KLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELA--------KPLP 249
           K+ HL  DL +PP FR  +C  +P  FR V    G ALEL  WD ELA        +   
Sbjct: 189 KVAHLKHDLGLPPEFRDTVCLRYPQYFRVVRMDRGPALELTHWDPELAVSAAELAEEESR 248

Query: 250 CPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKESI 309
             +   R LI+DRPLKFN ++L KGL L R     + +F+++P + PY    + L   S 
Sbjct: 249 AREAEERNLIIDRPLKFNRVRLPKGLKLTRGEARRIARFKEMPYISPY-ADFSHLRSGSD 308

Query: 310 ESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQRD 369
           E EK AC VV EIL + VEKRTL+DHLTHFR++F     LRGMI+RHP++FYVS KG RD
Sbjct: 309 EKEKHACGVVHEILSLTVEKRTLVDHLTHFREEFRFSQSLRGMIIRHPDMFYVSFKGDRD 368

Query: 370 SVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREK-GKAQIYDSKYGNYHEDNN 429
           SVFL E + D   LV+K++ +++K +   LV   +  RR   G  +  +   G+    + 
Sbjct: 369 SVFLREAYKD-SQLVEKNQLVLLKEKMRALVAVPRFPRRAAVGTGEEAEGMNGSLQSRDQ 428

Query: 430 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINAD 472
                    D++YDD  E L + ED   E       +    GD W   N D
Sbjct: 429 VS-------DEEYDDEDEGLSDMEDLISELSGGKSDADYEWGDGWFGENDD 459

BLAST of MC05g0802.1 vs. ExPASy Swiss-Prot
Match: Q65XL5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0571100 PE=2 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.3e-55
Identity = 154/429 (35.90%), Postives = 221/429 (51.52%), Query Frame = 0

Query: 70  KHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYPSIFELFSIP 129
           +  K + V KL  +L+S P   + L  L + R  L L + R L++++ R+P +FE+    
Sbjct: 75  RDKKLKLVLKLRNILVSNPDRVMSLRDLGRFRRDLGLTRKRRLIALLKRFPGVFEVVE-- 134

Query: 130 YPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLMLSSHHRILLS 189
                     +Y  L  RLTPAA  L   +  LK         KL+KLLM+S   RIL+ 
Sbjct: 135 --------EGVY-SLRFRLTPAAERLYLDELHLKNESEGLAVTKLRKLLMMSQDKRILIE 194

Query: 190 KLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELA--------KPLP 249
           K+ HL  DL +PP FR  +C  +P  FR V    G  LEL  WD ELA        +   
Sbjct: 195 KIAHLKNDLGLPPEFRDTICLRYPQYFRVVQMDRGPGLELTHWDPELAVSAAEVAEEENR 254

Query: 250 CPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKESI 309
             +   R LI+DRPLKFN +KL +GL L R     + +F+++P + PY +  + L   S 
Sbjct: 255 AREEQERNLIIDRPLKFNRVKLPQGLKLSRGEARRVAQFKEMPYISPY-SDFSHLRSGSA 314

Query: 310 ESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQRD 369
           E EK AC VV EIL + +EKRTL+DHLTHFR++F     LRGM++RHP++FYVSLKG RD
Sbjct: 315 EKEKHACGVVHEILSLTLEKRTLVDHLTHFREEFRFSQSLRGMLIRHPDMFYVSLKGDRD 374

Query: 370 SVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNNH 429
           SVFL E + +   LV+K + +++K +   LV   +  RR         S+  +       
Sbjct: 375 SVFLREAYKN-SQLVEKSKLVLLKEKMRALVAVPRFPRRGVPAT----SEEADRTNGAAQ 434

Query: 430 DYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSN-----GDFWTTINADI---I 483
                +D +DD D+G         SD E+   ++   +S+     GD W   N D     
Sbjct: 435 MLSEGSDVEDDEDEGL--------SDMEDLISEISGGKSDTDYHWGDGWVGENDDSPPDF 478

BLAST of MC05g0802.1 vs. ExPASy Swiss-Prot
Match: A0MFS5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01037 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.6e-54
Identity = 154/441 (34.92%), Postives = 227/441 (51.47%), Query Frame = 0

Query: 45  KPRHISSPC-SSLKIVRSHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGY 104
           K R +  P  +++K  +  + D    +  K + V  +  +L+S+P   + L  L K R  
Sbjct: 64  KTRVVVEPVRAAVKRRKELTFDSVVQRDKKLKLVLNIRKILVSQPDRMMSLRGLGKYRRD 123

Query: 105 LALHKPRSLLSMIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLK 164
           L L K R  ++++ +YP +FE+             +    L  ++T  A  L   +  ++
Sbjct: 124 LGLKKRRRFIALLRKYPGVFEI-----------VEEGAYSLRFKMTSEAERLYLDEMRIR 183

Query: 165 LAISNTLAEKLQKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSY 224
             + + L  KL+KL+M+S   RILL K+ HL  DL +P  FR  +C  +P  FR V T  
Sbjct: 184 NELEDVLVVKLRKLVMMSIDKRILLEKISHLKTDLGLPLEFRDTICQRYPQYFRVVPTPR 243

Query: 225 GRALELVSWDSELAKPLP--------CPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQE 284
           G ALEL  WD ELA              +   R LI+DRP KFN +KL +GLNL ++   
Sbjct: 244 GPALELTHWDPELAVSAAELSEDDNRTRESEERNLIIDRPPKFNRVKLPRGLNLSKSETR 303

Query: 285 FLIKFRDLPDVCPYKTPATELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDF 344
            + +FRD+  + PYK   + L   ++E EK AC V+ E+L +  EKRTL+DHLTHFR++F
Sbjct: 304 KISQFRDMQYISPYK-DFSHLRSGTLEKEKHACGVIHELLSLTTEKRTLVDHLTHFREEF 363

Query: 345 ELPNKLRGMIVRHPELFYVSLKGQRDSVFLVEGFDDKGVLVDKDETMVIKNQWMKLV--- 404
               +LRGM++RHP+LFYVSLKG+RDSVFL E + +   L+DKD   ++K +   LV   
Sbjct: 364 RFSQQLRGMLIRHPDLFYVSLKGERDSVFLREAYRN-SELIDKDPLTLVKEKMRALVSVP 423

Query: 405 ----REGKRMRREKGKAQIYDSKYGNYHEDNNHDYEVETDY-----------DDD----Y 455
               R G R   E  + +I  S      E+   D E  +D            DDD     
Sbjct: 424 RFPRRGGPRKDEEGREVEIDGSDADGEEEEEESDAEEWSDVDGYLEGEDGGNDDDGDWTD 483

BLAST of MC05g0802.1 vs. ExPASy Swiss-Prot
Match: Q689D6 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=RPD1 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 5.1e-18
Identity = 99/387 (25.58%), Postives = 180/387 (46.51%), Query Frame = 0

Query: 59  VRSHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLAL----HKPRSLLS 118
           VR H  D +     K R V K   L+LS+P H I + +L      L L    H+P + L 
Sbjct: 47  VRDHGYDNYMEVEKKIRKVVKFHSLILSQPNHTIAISLLDTLARRLGLGFKQHEPGAFLL 106

Query: 119 MIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKL 178
              ++P +FE++  P          +   L  RLT  A    R + +  L        +L
Sbjct: 107 ---KFPHVFEIYEHP----------VQRILYCRLTRKALDQIRHEHEAVLDQIPDAVTRL 166

Query: 179 QKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVD--TSYGRALELVSW 238
           +KL+M+S+  RI L  +     +  +P +F   +    P  FR +D   +  + +E+V  
Sbjct: 167 RKLVMMSNTGRIRLEHVRIARTEFGLPEDFEYSVILKHPQFFRLIDGEETRDKYIEIVEK 226

Query: 239 DSELAKPLPCPQVPSREL------IVDRPLKFN-LLKLRKGLNLKRAHQEFLIKFRDLPD 298
           D  L+    C     RE+      I    ++F+ ++    G  + +  +  + K++ LP 
Sbjct: 227 DPNLS---ICAIERVREIEYRTKGIDAEDVRFSFVVNFPPGFKIGKYFRIAVWKWQRLPY 286

Query: 299 VCPYK-TPATELAKESIES--EKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLR 358
             PY+     +L     ++  EKR+ A + E+L + VEK+  ++ + HFR    LP KL+
Sbjct: 287 WSPYEDISGYDLRSMEAQNRLEKRSVACIHELLSLTVEKKITLERIAHFRNVMNLPKKLK 346

Query: 359 GMIVRHPELFYVSLK---GQRDSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMR 418
             +++H  +FY+S +   G+  +VFL EG+  +G LV+ ++  + + +  +LV    R  
Sbjct: 347 EFLLQHQGIFYISTRGNYGKLHTVFLREGY-KRGELVEPNDVYLARRRLAELVLMSPR-- 406

Query: 419 REKGKAQIYDSKYGNYHEDNNHDYEVE 427
               KA++ D++   Y +  + + +VE
Sbjct: 407 ----KAKV-DAELVRYRDGLDDEDDVE 409

BLAST of MC05g0802.1 vs. ExPASy Swiss-Prot
Match: Q9ZUZ6 (Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=WTF9 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.4e-15
Identity = 82/339 (24.19%), Postives = 156/339 (46.02%), Query Frame = 0

Query: 67  HAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYPSIFELF 126
           H ++ ++ + V  L   ++ +P   IP+  +SK      +     +   + ++PSIFE F
Sbjct: 42  HILRSSQLKSVVSLKNCIVQEPNRCIPISAISKKTRQFDV--STKIAHFLRKFPSIFEEF 101

Query: 127 SIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLMLSSHHRI 186
             P    P            RLTP A  L RQ+  +    ++ L ++L+KL+++S  + +
Sbjct: 102 VGPEYNLP----------WFRLTPEATELDRQERVVYQTSADDLRDRLKKLILMSKDNVL 161

Query: 187 LLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTS---YGRALELVSWDSELAKPLPCP 246
            LS +  +   L +P ++      +    FR VD      G A++    D  L+      
Sbjct: 162 PLSIVQGMKWYLGLPDDYLQFPDMNLDSSFRFVDMEDGVKGLAVDYNGGDKVLSVLQKNA 221

Query: 247 QVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKESIES 306
               R  +    ++F L    KG  L+   +++L++F+ LP V PY   +  L   S  +
Sbjct: 222 MKKRRGEVSLEEIEFPLFP-SKGCRLRVKIEDWLMEFQKLPYVSPYDDYSC-LDPSSDIA 281

Query: 307 EKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQRDSV 366
           EKR    + E+L + VE       L   +K F LP K+     RHP++FY+S+K +  + 
Sbjct: 282 EKRVVGFLHELLCLFVEHSAERKKLLCLKKHFGLPQKVHKAFERHPQIFYLSMKNKTCTA 341

Query: 367 FLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREK 403
            L E + DK   V+    + ++ ++++L++  + + + +
Sbjct: 342 ILREPYRDK-ASVETHPVLGVRKKYIQLMKNSELILKSR 365

BLAST of MC05g0802.1 vs. NCBI nr
Match: XP_022145187.1 (protein ROOT PRIMORDIUM DEFECTIVE 1 [Momordica charantia] >XP_022145188.1 protein ROOT PRIMORDIUM DEFECTIVE 1 [Momordica charantia])

HSP 1 Score: 969 bits (2506), Expect = 0.0
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR 60
           MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR
Sbjct: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR 60

Query: 61  SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP 120
           SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP
Sbjct: 61  SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL 240

Query: 241 PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES 300
           PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES
Sbjct: 241 PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES 300

Query: 301 IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR 360
           IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN 420
           DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN
Sbjct: 361 DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN 420

Query: 421 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINADIIDDADGGH 480
           HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINADIIDDADGGH
Sbjct: 421 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINADIIDDADGGH 480

Query: 481 IEPW 484
           IEPW
Sbjct: 481 IEPW 484

BLAST of MC05g0802.1 vs. NCBI nr
Match: XP_038904265.1 (protein WHAT'S THIS FACTOR 1, chloroplastic [Benincasa hispida] >XP_038904305.1 protein WHAT'S THIS FACTOR 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 843 bits (2179), Expect = 1.71e-306
Identity = 418/484 (86.36%), Postives = 451/484 (93.18%), Query Frame = 0

Query: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR 60
           M LSSHF T  DWH+T LSSN L GNPLWLHSN+ CKYQRK+NF+ R+  +PCSSLKIVR
Sbjct: 1   MVLSSHFLTPTDWHVTLLSSNLLCGNPLWLHSNVSCKYQRKENFETRYTLTPCSSLKIVR 60

Query: 61  SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP 120
           S SLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYL+L +PRSLLSMIHRYP
Sbjct: 61  SRSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLSLPRPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATK+YPQLCVRLTPAAASLA+QDSDLKL ISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKVYPQLCVRLTPAAASLAKQDSDLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCND+P+KFRTVDTSYGRALELVSWD ELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVSWDPELAKPL 240

Query: 241 PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES 300
           PC QVPSRELIVDRPLKFNLL+LRKGLNLKRAHQEFLIKFRDLPDVCPYKTPA+ELAKES
Sbjct: 241 PCLQVPSRELIVDRPLKFNLLRLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR 360
           +ESEKRACAVVRE+LGMM+EKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN 420
           DSVFLVEGFD+KGVLV KDET+ IKNQWMKL+ EGKRMR+EK KA+ YDSKYGN H+++N
Sbjct: 361 DSVFLVEGFDEKGVLVKKDETLAIKNQWMKLLMEGKRMRQEKKKARKYDSKYGNEHKNDN 420

Query: 421 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINADIIDDADGGH 480
           HD+E+ETD+DDDYDDGFESLF++ED DFE+E  DL S+  NGDFW T N DII+DADGG 
Sbjct: 421 HDHEMETDFDDDYDDGFESLFQYEDLDFEDERSDLPSNWLNGDFWITNNVDIINDADGGR 480

Query: 481 IEPW 484
           IEPW
Sbjct: 481 IEPW 484

BLAST of MC05g0802.1 vs. NCBI nr
Match: XP_023527715.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 834 bits (2154), Expect = 1.14e-302
Identity = 417/485 (85.98%), Postives = 452/485 (93.20%), Query Frame = 0

Query: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR 60
           MALSS F T RDW++T LSSN LRG  LWLHSN+GCKYQRK+NFK  +  +P +S+KIVR
Sbjct: 1   MALSSQFLTPRDWYVTPLSSNLLRGTTLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60

Query: 61  SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP 120
           S  LDRHAVKHNKTRFVQKLIILLLSKPKHYIP+HILSKCRGYL+L KPRSLLSMIHRYP
Sbjct: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLA+QDS+LKL ISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLAKQDSNLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCND+P+KFRTVDTSYGRALELV WD ELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240

Query: 241 PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES 300
           PC QV SRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPA+ELAKES
Sbjct: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR 360
           +ESEKRACAVVRE+LGMM+EKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN 420
           DSVFLVEGF+DKG L++KDET+ IKNQWMKL+ EGKRMRREK KAQIYDS+YGN HE+ +
Sbjct: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENYS 420

Query: 421 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINA-DIIDDADGG 480
           HD+E+ETDYDDDY+DGFESLF++ED DFE+E  DL S+RSNGDFWTT NA DII+DA+GG
Sbjct: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINDANGG 480

Query: 481 HIEPW 484
           HIEPW
Sbjct: 481 HIEPW 485

BLAST of MC05g0802.1 vs. NCBI nr
Match: XP_022955645.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita moschata] >XP_022955646.1 protein WHAT'S THIS FACTOR 1 [Cucurbita moschata] >KAG7018490.1 Protein ROOT PRIMORDIUM DEFECTIVE 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 832 bits (2148), Expect = 9.34e-302
Identity = 415/485 (85.57%), Postives = 451/485 (92.99%), Query Frame = 0

Query: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR 60
           MALSS F T RDWH+TSLSSN L G PLWLHSN+GCKYQRK+NFK  +  +P +S+KIVR
Sbjct: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60

Query: 61  SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP 120
           S  LDRHAVKHNKTRFVQKLIILLLSKPKHYIP+HILSKCRGYL+L KPRSLLSMIHRYP
Sbjct: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTP AASLA+QDS+LKL ISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCND+P+KFRTVDTSYGRALELV WD ELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240

Query: 241 PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES 300
           PC QV SRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPA+ELAKES
Sbjct: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR 360
           +ESEKRACAVVRE+LGMM+EKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN 420
           DSVFLVEGF+DKG L++KDET+ IKNQWMKL+ EGKRMRREK KAQIYDS+YGN HE+++
Sbjct: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420

Query: 421 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINA-DIIDDADGG 480
           HD+E+ETDYDDDY+DGFESLF++ED DFE+E  DL S+RSNGDFWTT NA DII+  +GG
Sbjct: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480

Query: 481 HIEPW 484
           HI+PW
Sbjct: 481 HIKPW 485

BLAST of MC05g0802.1 vs. NCBI nr
Match: XP_004152293.1 (protein WHAT'S THIS FACTOR 1, chloroplastic [Cucumis sativus] >XP_031739932.1 protein WHAT'S THIS FACTOR 1, chloroplastic [Cucumis sativus] >KGN53093.1 hypothetical protein Csa_015066 [Cucumis sativus])

HSP 1 Score: 831 bits (2147), Expect = 1.28e-301
Identity = 411/484 (84.92%), Postives = 446/484 (92.15%), Query Frame = 0

Query: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR 60
           MAL SHF T  DWH+TSLSSN L G+PLWLHS +  K QRK+NF+  +  +PCSS+KIVR
Sbjct: 1   MALCSHFLTPTDWHVTSLSSNLLSGSPLWLHSKVDFKCQRKENFRTHYTLTPCSSIKIVR 60

Query: 61  SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP 120
           S SLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYL+L +PRSLLSMIHRYP
Sbjct: 61  SRSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLSLPRPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAAS+A+QDSDLK+ ISN LAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASIAKQDSDLKMVISNKLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL 240
           SSHHRILLSKLVHLAPDLS+PPNFRSRLCND+P+KFRTVDTSYGRALELVSWD ELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSLPPNFRSRLCNDYPEKFRTVDTSYGRALELVSWDPELAKPL 240

Query: 241 PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES 300
           PC QVPSRELIVDRPLKFNLL+LRKGLNLKR HQEFLIKFRDLPDVCPYK PA+ELAKES
Sbjct: 241 PCIQVPSRELIVDRPLKFNLLRLRKGLNLKRTHQEFLIKFRDLPDVCPYKNPASELAKES 300

Query: 301 IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR 360
           +ESEKRACAVVRE+LGMMVEKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMVEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN 420
           DSVFLVEGFDDKGVL++KDET+ IKNQWM L++E KR+RREK KAQIYDSKYGN HE+NN
Sbjct: 361 DSVFLVEGFDDKGVLLEKDETLAIKNQWMTLLKEAKRLRREKKKAQIYDSKYGNDHENNN 420

Query: 421 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINADIIDDADGGH 480
           HD+E+E DYDDDYDDGFESLF++ED DFE+E+  + S  SNGDFWTT N DI +DADGGH
Sbjct: 421 HDHEMENDYDDDYDDGFESLFQYEDLDFEDENSGVPSIWSNGDFWTTNNVDISNDADGGH 480

Query: 481 IEPW 484
           IEPW
Sbjct: 481 IEPW 484

BLAST of MC05g0802.1 vs. ExPASy TrEMBL
Match: A0A6J1CVW2 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Momordica charantia OX=3673 GN=LOC111014693 PE=4 SV=1)

HSP 1 Score: 969 bits (2506), Expect = 0.0
Identity = 484/484 (100.00%), Postives = 484/484 (100.00%), Query Frame = 0

Query: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR 60
           MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR
Sbjct: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR 60

Query: 61  SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP 120
           SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP
Sbjct: 61  SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL 240

Query: 241 PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES 300
           PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES
Sbjct: 241 PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES 300

Query: 301 IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR 360
           IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN 420
           DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN
Sbjct: 361 DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN 420

Query: 421 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINADIIDDADGGH 480
           HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINADIIDDADGGH
Sbjct: 421 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINADIIDDADGGH 480

Query: 481 IEPW 484
           IEPW
Sbjct: 481 IEPW 484

BLAST of MC05g0802.1 vs. ExPASy TrEMBL
Match: A0A6J1GVP1 (protein WHAT'S THIS FACTOR 1 OS=Cucurbita moschata OX=3662 GN=LOC111457587 PE=4 SV=1)

HSP 1 Score: 832 bits (2148), Expect = 4.52e-302
Identity = 415/485 (85.57%), Postives = 451/485 (92.99%), Query Frame = 0

Query: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR 60
           MALSS F T RDWH+TSLSSN L G PLWLHSN+GCKYQRK+NFK  +  +P +S+KIVR
Sbjct: 1   MALSSQFLTPRDWHVTSLSSNLLCGTPLWLHSNVGCKYQRKENFKTCYTLTPRASVKIVR 60

Query: 61  SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP 120
           S  LDRHAVKHNKTRFVQKLIILLLSKPKHYIP+HILSKCRGYL+L KPRSLLSMIHRYP
Sbjct: 61  SRPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTP AASLA+QDS+LKL ISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPVAASLAKQDSNLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCND+P+KFRTVDTSYGRALELV WD ELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKPL 240

Query: 241 PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES 300
           PC QV SRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPA+ELAKES
Sbjct: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR 360
           +ESEKRACAVVRE+LGMM+EKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN 420
           DSVFLVEGF+DKG L++KDET+ IKNQWMKL+ EGKRMRREK KAQIYDS+YGN HE+++
Sbjct: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420

Query: 421 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINA-DIIDDADGG 480
           HD+E+ETDYDDDY+DGFESLF++ED DFE+E  DL S+RSNGDFWTT NA DII+  +GG
Sbjct: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADIINGENGG 480

Query: 481 HIEPW 484
           HI+PW
Sbjct: 481 HIKPW 485

BLAST of MC05g0802.1 vs. ExPASy TrEMBL
Match: A0A0A0KU86 (PORR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G015830 PE=4 SV=1)

HSP 1 Score: 831 bits (2147), Expect = 6.19e-302
Identity = 411/484 (84.92%), Postives = 446/484 (92.15%), Query Frame = 0

Query: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR 60
           MAL SHF T  DWH+TSLSSN L G+PLWLHS +  K QRK+NF+  +  +PCSS+KIVR
Sbjct: 1   MALCSHFLTPTDWHVTSLSSNLLSGSPLWLHSKVDFKCQRKENFRTHYTLTPCSSIKIVR 60

Query: 61  SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP 120
           S SLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYL+L +PRSLLSMIHRYP
Sbjct: 61  SRSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLSLPRPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAAS+A+QDSDLK+ ISN LAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASIAKQDSDLKMVISNKLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL 240
           SSHHRILLSKLVHLAPDLS+PPNFRSRLCND+P+KFRTVDTSYGRALELVSWD ELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSLPPNFRSRLCNDYPEKFRTVDTSYGRALELVSWDPELAKPL 240

Query: 241 PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES 300
           PC QVPSRELIVDRPLKFNLL+LRKGLNLKR HQEFLIKFRDLPDVCPYK PA+ELAKES
Sbjct: 241 PCIQVPSRELIVDRPLKFNLLRLRKGLNLKRTHQEFLIKFRDLPDVCPYKNPASELAKES 300

Query: 301 IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR 360
           +ESEKRACAVVRE+LGMMVEKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMVEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN 420
           DSVFLVEGFDDKGVL++KDET+ IKNQWM L++E KR+RREK KAQIYDSKYGN HE+NN
Sbjct: 361 DSVFLVEGFDDKGVLLEKDETLAIKNQWMTLLKEAKRLRREKKKAQIYDSKYGNDHENNN 420

Query: 421 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINADIIDDADGGH 480
           HD+E+E DYDDDYDDGFESLF++ED DFE+E+  + S  SNGDFWTT N DI +DADGGH
Sbjct: 421 HDHEMENDYDDDYDDGFESLFQYEDLDFEDENSGVPSIWSNGDFWTTNNVDISNDADGGH 480

Query: 481 IEPW 484
           IEPW
Sbjct: 481 IEPW 484

BLAST of MC05g0802.1 vs. ExPASy TrEMBL
Match: A0A6J1IUA5 (protein WHAT'S THIS FACTOR 1 OS=Cucurbita maxima OX=3661 GN=LOC111479376 PE=4 SV=1)

HSP 1 Score: 828 bits (2140), Expect = 7.48e-301
Identity = 414/485 (85.36%), Postives = 450/485 (92.78%), Query Frame = 0

Query: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR 60
           MALSS F T RDWH+TS SSN LRG PLWLHSN+GCKYQRK+NF+  +  +P +S+KIVR
Sbjct: 1   MALSSQFLTPRDWHVTSRSSNLLRGTPLWLHSNVGCKYQRKENFETSYTLTPRASVKIVR 60

Query: 61  SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP 120
           S  LDRHAVKHNKTRFVQKLIILLLSKPKHYIP+HILSKCRGYL+L KPRSLLSMIHRYP
Sbjct: 61  SCPLDRHAVKHNKTRFVQKLIILLLSKPKHYIPIHILSKCRGYLSLPKPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLA+QDS+LKL ISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLAKQDSNLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCND+P+KFRTVDTSYGRALELV WD ELAK L
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVFWDPELAKTL 240

Query: 241 PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES 300
           PC QV SRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPA+ELAKES
Sbjct: 241 PCLQVSSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR 360
           +ESEKRACAVVRE+LGMM+EKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN 420
           DSVFLVEGF+DKG L++KDET+ IKNQWMKL+ EGKRMRREK KAQIYDS+YGN HE+++
Sbjct: 361 DSVFLVEGFNDKGHLIEKDETLAIKNQWMKLLMEGKRMRREKRKAQIYDSRYGNDHENHS 420

Query: 421 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINA-DIIDDADGG 480
           HD+E+ETDYDDDY+DGFESLF++ED DFE+E  DL S+RSNGDFWTT NA DI +  +GG
Sbjct: 421 HDHEMETDYDDDYEDGFESLFQYEDLDFEDEESDLPSNRSNGDFWTTNNAADITNGENGG 480

Query: 481 HIEPW 484
           HIEPW
Sbjct: 481 HIEPW 485

BLAST of MC05g0802.1 vs. ExPASy TrEMBL
Match: A0A1S3BYB2 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo OX=3656 GN=LOC103494529 PE=4 SV=1)

HSP 1 Score: 819 bits (2115), Expect = 4.45e-297
Identity = 409/484 (84.50%), Postives = 442/484 (91.32%), Query Frame = 0

Query: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWLHSNIGCKYQRKQNFKPRHISSPCSSLKIVR 60
           MALSSHF T  DWH  SLSSN L G+PLWLHS +  K QRK+NF   +  +PCSS+KIVR
Sbjct: 1   MALSSHFLTPADWH-ASLSSNLLSGSPLWLHSKVDLKCQRKENFITHYTLTPCSSIKIVR 60

Query: 61  SHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRYP 120
           S SLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYL+L +PRSLLSMIHRYP
Sbjct: 61  SRSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLSLPRPRSLLSMIHRYP 120

Query: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLML 180
           SIFELFSIPYPPTPLNATKLYPQLCVRLT AAAS+A+QDSDLKL ISNTLAEKLQKLLML
Sbjct: 121 SIFELFSIPYPPTPLNATKLYPQLCVRLTAAAASIAKQDSDLKLVISNTLAEKLQKLLML 180

Query: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKPL 240
           SSHHRILLSKLVHLAPDLSMPPNFRSRLCND+P+KFRTVDTSYGRALELVSWD ELAKPL
Sbjct: 181 SSHHRILLSKLVHLAPDLSMPPNFRSRLCNDYPEKFRTVDTSYGRALELVSWDPELAKPL 240

Query: 241 PCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKES 300
           PC QVPSRELIVDRPLKFNLL+LRKGLNLKR HQEFLIKFRDLPDVCPYKTPA+ELAKES
Sbjct: 241 PCIQVPSRELIVDRPLKFNLLRLRKGLNLKRTHQEFLIKFRDLPDVCPYKTPASELAKES 300

Query: 301 IESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQR 360
           +ESEKRACAVVRE+LGMM+EKRTLIDHLTHFRKDF LPNKLRGMIVRHPELFYVSLKGQR
Sbjct: 301 LESEKRACAVVREVLGMMIEKRTLIDHLTHFRKDFGLPNKLRGMIVRHPELFYVSLKGQR 360

Query: 361 DSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGNYHEDNN 420
           DSVFLVEGFDDKG L++KDET+ IKN+WM L++EGKRMRREK KAQIYDSKYGN HE+NN
Sbjct: 361 DSVFLVEGFDDKGALLEKDETLAIKNRWMALLKEGKRMRREKKKAQIYDSKYGNDHENNN 420

Query: 421 HDYEVETDYDDDYDDGFESLFEFEDSDFENESDDLLSSRSNGDFWTTINADIIDDADGGH 480
           HD+E+E DYDD+YDDGFESLF++ED DFE+E+  + S  SNGDFWTT N DI +D D  H
Sbjct: 421 HDHEMENDYDDNYDDGFESLFQYEDLDFEDENSGVPSIWSNGDFWTTNNVDISNDTDESH 480

Query: 481 IEPW 484
           IEPW
Sbjct: 481 IEPW 483

BLAST of MC05g0802.1 vs. TAIR 10
Match: AT5G62990.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 503.4 bits (1295), Expect = 2.0e-142
Identity = 289/496 (58.27%), Postives = 357/496 (71.98%), Query Frame = 0

Query: 1   MALSSHFSTSRDWHLTSLSSNFLRGNPLWL-HSNIGCKYQRKQNFKPRHISSPCSSLKIV 60
           MAL+    +S +  L   SS FL   PL +  SN+  K   K+      ++  CS+ KIV
Sbjct: 3   MALTQLVPSSPE-KLFCFSSFFLPSIPLEIRRSNVTTKTSSKK-LGSLSLNISCSAHKIV 62

Query: 61  RSHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSMIHRY 120
           RS SLDRH VK N+ RFVQKL  LLLSKPKHYIP+ IL KCR YL +  P ++LSMI RY
Sbjct: 63  RSPSLDRHVVKQNRVRFVQKLNTLLLSKPKHYIPIEILYKCRSYLCIENPLAILSMIRRY 122

Query: 121 PSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLLM 180
           P+IFELF+ P P  P+NATK   QLCVRLT AA+SLA Q+ +LK  IS+ LA KLQKLLM
Sbjct: 123 PTIFELFTTPTPHLPMNATKPLSQLCVRLTSAASSLAMQELNLKSEISDKLATKLQKLLM 182

Query: 181 LSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSELAKP 240
           LSSH R+LLSKLVH+APD   PPNFRSRLCND+PDKF+TVDTSYGRALELVS D ELA  
Sbjct: 183 LSSHRRLLLSKLVHIAPDFGFPPNFRSRLCNDYPDKFKTVDTSYGRALELVSSDPELANQ 242

Query: 241 LPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPATELAKE 300
           +P P+V  R LIVDRPLKF  L LR+GLNLKR HQ FLIKFR+ PDVCPYK  +  LA E
Sbjct: 243 MPSPEV-DRGLIVDRPLKFKRLNLRRGLNLKRRHQGFLIKFRESPDVCPYKMSSDYLASE 302

Query: 301 SIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYVSLKGQ 360
           SIE+EKRACAVVRE+LG+ VEKRTLIDHLTHFRK+F LPNKLR +IVRHPELFYVS+KG 
Sbjct: 303 SIEAEKRACAVVREVLGLTVEKRTLIDHLTHFRKEFSLPNKLRDLIVRHPELFYVSIKGM 362

Query: 361 RDSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGK--AQIYDSKYGNYHE 420
           RDSVFLVE ++D G L+DKDE +VI+ + + L++EGKR+RRE+ +  A + D       +
Sbjct: 363 RDSVFLVEAYNDNGDLLDKDERLVIRERLIDLIQEGKRIRRERRRKGATLGDKSVEEKRK 422

Query: 421 DNNHDYEVETDYDDDYDDGFESLFEFEDSDFE---NESDDLLSSRSNG---DFWT---TI 480
           D+    + ++D DD+Y+DGFE+LF+ ED   E   +E DD   +  NG   ++W+   + 
Sbjct: 423 DDETIDDYDSDLDDEYEDGFENLFDSEDLGVEYHFDEEDDDDEAGVNGESVEYWSRKLSS 482

Query: 481 NADIIDDADGGHIEPW 485
           +    D+A+   +E W
Sbjct: 483 SGSSSDEANSA-VESW 494

BLAST of MC05g0802.1 vs. TAIR 10
Match: AT4G01037.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-55
Identity = 154/441 (34.92%), Postives = 227/441 (51.47%), Query Frame = 0

Query: 45  KPRHISSPC-SSLKIVRSHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGY 104
           K R +  P  +++K  +  + D    +  K + V  +  +L+S+P   + L  L K R  
Sbjct: 64  KTRVVVEPVRAAVKRRKELTFDSVVQRDKKLKLVLNIRKILVSQPDRMMSLRGLGKYRRD 123

Query: 105 LALHKPRSLLSMIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLK 164
           L L K R  ++++ +YP +FE+             +    L  ++T  A  L   +  ++
Sbjct: 124 LGLKKRRRFIALLRKYPGVFEI-----------VEEGAYSLRFKMTSEAERLYLDEMRIR 183

Query: 165 LAISNTLAEKLQKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSY 224
             + + L  KL+KL+M+S   RILL K+ HL  DL +P  FR  +C  +P  FR V T  
Sbjct: 184 NELEDVLVVKLRKLVMMSIDKRILLEKISHLKTDLGLPLEFRDTICQRYPQYFRVVPTPR 243

Query: 225 GRALELVSWDSELAKPLP--------CPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQE 284
           G ALEL  WD ELA              +   R LI+DRP KFN +KL +GLNL ++   
Sbjct: 244 GPALELTHWDPELAVSAAELSEDDNRTRESEERNLIIDRPPKFNRVKLPRGLNLSKSETR 303

Query: 285 FLIKFRDLPDVCPYKTPATELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDF 344
            + +FRD+  + PYK   + L   ++E EK AC V+ E+L +  EKRTL+DHLTHFR++F
Sbjct: 304 KISQFRDMQYISPYK-DFSHLRSGTLEKEKHACGVIHELLSLTTEKRTLVDHLTHFREEF 363

Query: 345 ELPNKLRGMIVRHPELFYVSLKGQRDSVFLVEGFDDKGVLVDKDETMVIKNQWMKLV--- 404
               +LRGM++RHP+LFYVSLKG+RDSVFL E + +   L+DKD   ++K +   LV   
Sbjct: 364 RFSQQLRGMLIRHPDLFYVSLKGERDSVFLREAYRN-SELIDKDPLTLVKEKMRALVSVP 423

Query: 405 ----REGKRMRREKGKAQIYDSKYGNYHEDNNHDYEVETDY-----------DDD----Y 455
               R G R   E  + +I  S      E+   D E  +D            DDD     
Sbjct: 424 RFPRRGGPRKDEEGREVEIDGSDADGEEEEEESDAEEWSDVDGYLEGEDGGNDDDGDWTD 483

BLAST of MC05g0802.1 vs. TAIR 10
Match: AT3G63090.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 119.0 bits (297), Expect = 1.0e-26
Identity = 106/359 (29.53%), Postives = 175/359 (48.75%), Query Frame = 0

Query: 54  SSLKIV--RSHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLAL-HKPR 113
           SSLK+V  +   LD    +  + +   +++  +L++P   IPL  L K R  L L  K +
Sbjct: 32  SSLKVVWKKDTRLDEAIEQDKRYKLCARVVKEVLNEPGQVIPLRYLEKRRERLRLTFKAK 91

Query: 114 SLLSMIHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTL 173
           S + M    PS+FE++     P      K  P   +R TP   +   ++  +       L
Sbjct: 92  SFVEM---NPSLFEIYYDRIKP------KSDPVQFLRPTPRLRAFLDEEQRIYSENEPLL 151

Query: 174 AEKLQKLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFR-TVDTSYGRA-LE 233
             KL +LLM++    I   KLVH+  D   P +F  +L   +P+ FR T     G++ LE
Sbjct: 152 VSKLCQLLMMAKDKVISADKLVHVKRDFGFPNDFLMKLVQKYPNYFRLTGLPEEGKSFLE 211

Query: 234 LVSWDSELAKPLPCPQVPSRELIVDRPLKFNL-LKLRKGLNLKRAHQEFLIKFRDLPDVC 293
           LVSW+ + AK     +     L     ++ N  +KL  G  L++  +E+   + +   + 
Sbjct: 212 LVSWNPDFAKSKIELRAEDETLKTGVRIRPNYNVKLPSGFFLRKEMREWTRDWLEQDYIS 271

Query: 294 PYKTPATELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVR 353
           PY+   + L + S E EKR   VV E+L + + KR  +  L  F  +F   N    +  R
Sbjct: 272 PYE-DVSHLDQASKEMEKRTVGVVHELLSLSLLKRVPVPTLGKFCDEFRFSNAFSSVFTR 331

Query: 354 HPELFYVSLKGQRDSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQ 407
           H  +FY+SLKG   +  L E + D   LVD+D  + IK+++++L+ EG + R+++ K Q
Sbjct: 332 HSGIFYLSLKGGIKTAVLREAYKD-DELVDRDPLLAIKDKFLRLLEEGWQERKDRLKLQ 379

BLAST of MC05g0802.1 vs. TAIR 10
Match: AT5G48040.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 117.9 bits (294), Expect = 2.3e-26
Identity = 111/390 (28.46%), Postives = 183/390 (46.92%), Query Frame = 0

Query: 56  LKIVRSHSLDRHAVKHNKTRFVQKLIILLLSKPKHYIPLHILSKCRGYLALHKPRSLLSM 115
           LK V+   LD   V+    R V  L+ ++ + P   +P+  L   RG L L +   L + 
Sbjct: 37  LKWVKDRELDAVVVREKHLRAVCNLVSVISASPDLRLPIFKLLPHRGQLGLPQELKLSAF 96

Query: 116 IHRYPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQ 175
           I RYP+IF    + +       T +    C  LT     L  ++ D+       +  +L 
Sbjct: 97  IRRYPNIF----VEHCYWDSAGTSV---PCFGLTRETIDLYYEEVDVSRVNERDVLVRLC 156

Query: 176 KLLMLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYG-RALELVSWDS 235
           KLLML+    + L  + HL  DL +P ++R  L    PD F  V  S     L+L+ WD 
Sbjct: 157 KLLMLTCERTLSLHSIDHLRWDLGLPYDYRDSLITKHPDLFSLVKLSSDLDGLKLIHWDE 216

Query: 236 ELAKPLPCPQVPSRELIVDRPLKFNLLKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTPAT 295
            LA      Q+  RE + +       +K  +G  LKR   E+L +++ LP   PY   A+
Sbjct: 217 HLA----VSQMQLREDVGNDERMAFPVKFTRGFGLKRKSIEWLQEWQRLPYTSPY-VDAS 276

Query: 296 ELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELFYV 355
            L   +  SEKR   V  E+L + + K+T   ++++ RK F LP K   +  RHP +FY+
Sbjct: 277 HLDPRTDLSEKRNVGVFHELLHLTIGKKTERKNVSNLRKPFALPQKFTKVFERHPGIFYI 336

Query: 356 SLKGQRDSVFLVEGFDDKGVLVDKDETMVIKNQWMKLVREGKRMRREKGKAQIYDSKYGN 415
           S+K    +V L E +D +  L++K   + ++ ++  ++ EG  + R +G   +Y      
Sbjct: 337 SMKCDTQTVILREAYDRRH-LIEKHPLVEVREKFANMMNEG-FLDRSRG---LYQKSVEA 396

Query: 416 YHEDNN--HDYEVETDYDDDYDDGFESLFE 443
             E NN    Y V +D +++ D+   S ++
Sbjct: 397 DLEKNNIKTSYPVWSDEEEELDNNLISGYD 409

BLAST of MC05g0802.1 vs. TAIR 10
Match: AT5G45790.2 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 114.4 bits (285), Expect = 2.6e-25
Identity = 103/390 (26.41%), Postives = 186/390 (47.69%), Query Frame = 0

Query: 60  RSHSLDRHAVKHNKTRFVQKLIILLLSKPK-HYIPLHILSKCRGYLALHKPRSLLSMIHR 119
           R H LD+   +  K   + ++  L+ SK +  ++ L ++S+ +  + L+   ++ + I +
Sbjct: 70  RDHQLDKIIPQIRKLNIILEISKLMSSKKRGPFVSLQLMSRWKNLVGLNV--NVGAFIGK 129

Query: 120 YPSIFELFSIPYPPTPLNATKLYPQLCVRLTPAAASLARQDSDLKLAISNTLAEKLQKLL 179
           YP  FE+F+ P+             LC ++T     L  ++ ++         ++++KLL
Sbjct: 130 YPHAFEIFTHPFS----------KNLCCKITEKFKVLIDEEENVVRECEVDAVKRVKKLL 189

Query: 180 MLSSHHRILLSKLVHLAPDLSMPPNFRSRLCNDFPDKFRTVDTSYGRALELVSWDSE--- 239
           +LS H  + +  L  +  +L +P +FR  +   +  +FR VD      LELV  D E   
Sbjct: 190 LLSKHGVLRVHALRLIRKELGLPEDFRDSILAKYSSEFRLVDL---ETLELVDRDDESLC 249

Query: 240 LAKPLPCPQVPSRELIVDRPLKFNL---LKLRKGLNLKRAHQEFLIKFRDLPDVCPYKTP 299
           +AK     +V  RE  + +  + N    + L  G  +++  +E L  ++ +P V PY   
Sbjct: 250 VAKVEEWREVEYREKWLSK-FETNYAFPIHLPTGFKIEKGFREELKNWQRVPYVKPY--D 309

Query: 300 ATELAKESIESEKRACAVVREILGMMVEKRTLIDHLTHFRKDFELPNKLRGMIVRHPELF 359
             E+++     EKR  AV+ E+L + VEK   ++ L HFRKD  +   +R +I++HP +F
Sbjct: 310 RKEISRGLERFEKRVVAVIHELLSLTVEKMVEVERLAHFRKDLGIEVNVREVILKHPGIF 369

Query: 360 YVSLKGQRDSVFLVEGFDDKGVLVDKDETMVIKNQWMKLV------------REGKRMRR 419
           YVS KG   ++FL E +  KG L++ +    ++ + + LV            RE +  R 
Sbjct: 370 YVSTKGSSQTLFLREAY-SKGCLIEPNPIYNVRRKMLDLVLLKTRYSRMLLQREDETHRE 429

Query: 420 EKGKAQIYDSKYGNYHEDNNHDYEVETDYD 431
           EK +  +  S         N D+E   D D
Sbjct: 430 EKSRDVLISS---------NEDWEGGRDGD 431