MC05g0054.1 (mRNA) Bitter gourd (Dali-11) v1

Overview
NameMC05g0054.1
TypemRNA
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionInhibitor of trypsin and hageman factor-like
LocationMC05: 407499 .. 407693 (-)
Sequence length195
RNA-Seq ExpressionMC05g0054.1
SyntenyMC05g0054.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAGAGTTCGTGGCCAGAGTTGGTGGGCAAATTGGGAAAGCACGCAGAGAAAACCATCGAGAGGGAGAATCGTTTGGTTGATGCAGTGATTGTTGGCGAAGGCTCAAGTGTGATTCTTGACTTCAGGTGCGACAGGGTGTGGGTTTGGGTGAACGAGAAAACTGGTAGAGTTACTAGGACTCCTATTATTGGT

mRNA sequence

GGAAAGAGTTCGTGGCCAGAGTTGGTGGGCAAATTGGGAAAGCACGCAGAGAAAACCATCGAGAGGGAGAATCGTTTGGTTGATGCAGTGATTGTTGGCGAAGGCTCAAGTGTGATTCTTGACTTCAGGTGCGACAGGGTGTGGGTTTGGGTGAACGAGAAAACTGGTAGAGTTACTAGGACTCCTATTATTGGT

Coding sequence (CDS)

GGAAAGAGTTCGTGGCCAGAGTTGGTGGGCAAATTGGGAAAGCACGCAGAGAAAACCATCGAGAGGGAGAATCGTTTGGTTGATGCAGTGATTGTTGGCGAAGGCTCAAGTGTGATTCTTGACTTCAGGTGCGACAGGGTGTGGGTTTGGGTGAACGAGAAAACTGGTAGAGTTACTAGGACTCCTATTATTGGT

Protein sequence

GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTRTPIIG
Homology
BLAST of MC05g0054.1 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.0e-14
Identity = 38/64 (59.38%), Postives = 47/64 (73.44%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GK++WPELVGK G  A  T+ERENR V A+++ EGS++  DFRCDRVWV VN+  G VT 
Sbjct: 6  GKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDH-GVVTS 65

Query: 61 TPII 65
           P I
Sbjct: 66 VPHI 68

BLAST of MC05g0054.1 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.9e-13
Identity = 40/65 (61.54%), Postives = 43/65 (66.15%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GK SWP+LVG  G  A+  IEREN  V AVIV  GS V  DFRCDRV VWV E+ G V R
Sbjct: 5  GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTER-GIVAR 64

Query: 61 TPIIG 66
           P IG
Sbjct: 65 PPAIG 68

BLAST of MC05g0054.1 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.8e-11
Identity = 37/65 (56.92%), Postives = 43/65 (66.15%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWP LVG  G  A+  IER+N  V AVI+ EG+ V  DFRC+RV +WVN K G V  
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVN-KRGLVVS 64

Query: 61 TPIIG 66
           P IG
Sbjct: 65 PPRIG 68

BLAST of MC05g0054.1 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.2e-10
Identity = 34/62 (54.84%), Postives = 39/62 (62.90%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GK  WPELVG+ G  A   IEREN  V +++  E S    DFRCDRVWV V + TG V R
Sbjct: 6  GKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWV-VVDSTGVVVR 65

Query: 61 TP 63
          TP
Sbjct: 66 TP 66

BLAST of MC05g0054.1 vs. ExPASy Swiss-Prot
Match: P86971 (Trypsin inhibitor OS=Fagopyrum tataricum OX=62330 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 4.4e-10
Identity = 34/69 (49.28%), Postives = 43/69 (62.32%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSV----ILDFRCDRVWVWVNEKTG 60
          GK SWPELVG  GK A  TI++EN  V AV+    +++      DFRCDRV V +N   G
Sbjct: 18 GKQSWPELVGTKGKTAAATIDKENTHVTAVLCPPLTTLAACRTFDFRCDRVRVLINRIGG 77

Query: 61 RVTRTPIIG 66
           VT+TP +G
Sbjct: 78 VVTKTPTVG 86

BLAST of MC05g0054.1 vs. NCBI nr
Match: KAA0049295.1 (inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa] >TYK17263.1 inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa])

HSP 1 Score: 111 bits (277), Expect = 2.50e-30
Identity = 53/65 (81.54%), Postives = 55/65 (84.62%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVGK GK AEKTIEREN LVDA+IV EGS VI DFRCDRVWVWV+ KT  VTR
Sbjct: 7  GKSSWPELVGKAGKEAEKTIERENPLVDAIIVDEGSVVIQDFRCDRVWVWVDPKTAIVTR 66

Query: 61 TPIIG 65
          TP IG
Sbjct: 67 TPFIG 71

BLAST of MC05g0054.1 vs. NCBI nr
Match: KAG6582359.1 (hypothetical protein SDJN03_22361, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 106 bits (265), Expect = 1.69e-28
Identity = 50/65 (76.92%), Postives = 54/65 (83.08%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVGK GK AE+ IE+EN LVDA++V EGS VI DFRCDRVWVWV  KTG VTR
Sbjct: 7  GKSSWPELVGKDGKEAERIIEKENPLVDAIVVDEGSVVIQDFRCDRVWVWVTVKTGIVTR 66

Query: 61 TPIIG 65
          TP IG
Sbjct: 67 TPFIG 71

BLAST of MC05g0054.1 vs. NCBI nr
Match: KAG6582360.1 (hypothetical protein SDJN03_22362, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 105 bits (262), Expect = 4.73e-28
Identity = 49/65 (75.38%), Postives = 55/65 (84.62%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVGK+GK AEK IE+EN LVDA+IV EGS+V  DFRCDRVWVWV+  T  VTR
Sbjct: 6  GKSSWPELVGKVGKDAEKIIEKENPLVDAIIVDEGSNVPFDFRCDRVWVWVSPTTKIVTR 65

Query: 61 TPIIG 65
          TP+IG
Sbjct: 66 TPVIG 70

BLAST of MC05g0054.1 vs. NCBI nr
Match: XP_004134090.1 (glu S.griseus protease inhibitor [Cucumis sativus])

HSP 1 Score: 104 bits (259), Expect = 1.39e-27
Identity = 50/65 (76.92%), Postives = 52/65 (80.00%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVGK GK AEK IE+EN  VDAVIV EGS V  DFRCDRVWVWV+ KT  VTR
Sbjct: 7  GKSSWPELVGKAGKEAEKIIEKENPSVDAVIVDEGSVVTQDFRCDRVWVWVDSKTSIVTR 66

Query: 61 TPIIG 65
          TP IG
Sbjct: 67 TPFIG 71

BLAST of MC05g0054.1 vs. NCBI nr
Match: KAF7837521.1 (inhibitor of trypsin and hageman factor-like protein [Senna tora])

HSP 1 Score: 102 bits (255), Expect = 5.53e-27
Identity = 51/65 (78.46%), Postives = 53/65 (81.54%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVG  GK AE TIEREN LVDA+IV EGSSVI DFRCDRVWVWVN   G VT+
Sbjct: 7  GKSSWPELVGVKGKTAEATIERENSLVDAIIVDEGSSVITDFRCDRVWVWVNSD-GYVTK 66

Query: 61 TPIIG 65
           PIIG
Sbjct: 67 VPIIG 70

BLAST of MC05g0054.1 vs. ExPASy TrEMBL
Match: A0A5D3CZE2 (Inhibitor of trypsin and hageman factor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001160 PE=3 SV=1)

HSP 1 Score: 111 bits (277), Expect = 1.21e-30
Identity = 53/65 (81.54%), Postives = 55/65 (84.62%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVGK GK AEKTIEREN LVDA+IV EGS VI DFRCDRVWVWV+ KT  VTR
Sbjct: 7  GKSSWPELVGKAGKEAEKTIERENPLVDAIIVDEGSVVIQDFRCDRVWVWVDPKTAIVTR 66

Query: 61 TPIIG 65
          TP IG
Sbjct: 67 TPFIG 71

BLAST of MC05g0054.1 vs. ExPASy TrEMBL
Match: A0A0A0L795 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142960 PE=3 SV=1)

HSP 1 Score: 104 bits (259), Expect = 6.75e-28
Identity = 50/65 (76.92%), Postives = 52/65 (80.00%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVGK GK AEK IE+EN  VDAVIV EGS V  DFRCDRVWVWV+ KT  VTR
Sbjct: 7  GKSSWPELVGKAGKEAEKIIEKENPSVDAVIVDEGSVVTQDFRCDRVWVWVDSKTSIVTR 66

Query: 61 TPIIG 65
          TP IG
Sbjct: 67 TPFIG 71

BLAST of MC05g0054.1 vs. ExPASy TrEMBL
Match: A0A067JP85 (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_21815 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 8.98e-26
Identity = 49/65 (75.38%), Postives = 53/65 (81.54%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPEL+G  GK A  TIE+EN  VDA++V EGSSVILDFRCDRVWVWVNE  G VTR
Sbjct: 7  GKSSWPELLGANGKAAAATIEKENGNVDAILVKEGSSVILDFRCDRVWVWVNEN-GVVTR 66

Query: 61 TPIIG 65
           PIIG
Sbjct: 67 VPIIG 70

BLAST of MC05g0054.1 vs. ExPASy TrEMBL
Match: A0A4Y7J9L1 (Uncharacterized protein OS=Papaver somniferum OX=3469 GN=C5167_005120 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 9.23e-26
Identity = 45/65 (69.23%), Postives = 55/65 (84.62%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GK+SWPELVG LGK AEK IE +NR V+A+++ EGS+V LDFRCDRVWVW+NE  G+V R
Sbjct: 8  GKNSWPELVGCLGKTAEKKIEYQNRNVNAILIPEGSAVTLDFRCDRVWVWINE-AGKVVR 67

Query: 61 TPIIG 65
          TP+IG
Sbjct: 68 TPVIG 71

BLAST of MC05g0054.1 vs. ExPASy TrEMBL
Match: A0A7N2LXD5 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 5.34e-25
Identity = 50/65 (76.92%), Postives = 51/65 (78.46%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVG  G  AE TIEREN  VDAVIV EGSSVILDFRCDRVWVWV +K G V R
Sbjct: 8  GKSSWPELVGVQGTVAEATIERENSYVDAVIVPEGSSVILDFRCDRVWVWV-DKNGIVYR 67

Query: 61 TPIIG 65
           P IG
Sbjct: 68 VPTIG 71

BLAST of MC05g0054.1 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 68.6 bits (166), Expect = 2.2e-12
Identity = 35/65 (53.85%), Postives = 46/65 (70.77%), Query Frame = 0

Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPEL+G  G+ A++ IEREN  + AVI+ +G+ V   F C RV+VWVN+  G V +
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVND-CGIVVQ 95

Query: 61 TPIIG 66
           PIIG
Sbjct: 96 IPIIG 99

BLAST of MC05g0054.1 vs. TAIR 10
Match: AT5G43570.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 65.1 bits (157), Expect = 2.4e-11
Identity = 33/62 (53.23%), Postives = 44/62 (70.97%), Query Frame = 0

Query: 2  KSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTRT 61
          KSSWPELVG+ G+  ++ I+REN  V A I+ E + V+    CDRV+V VN++ G VTRT
Sbjct: 14 KSSWPELVGRRGEEVKEIIDRENTKVTAKIISENAVVLAVVICDRVYVRVNDQ-GIVTRT 73

Query: 62 PI 64
          PI
Sbjct: 74 PI 74

BLAST of MC05g0054.1 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 61.2 bits (147), Expect = 3.5e-10
Identity = 32/64 (50.00%), Postives = 41/64 (64.06%), Query Frame = 0

Query: 2  KSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTRT 61
          K+SWPEL G  G +A   IEREN  V+A ++ +GS V  DFRCDRV V+V +    V +T
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFV-DGNRIVVKT 67

Query: 62 PIIG 66
          P  G
Sbjct: 68 PKSG 70

BLAST of MC05g0054.1 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 57.0 bits (136), Expect = 6.6e-09
Identity = 30/64 (46.88%), Postives = 42/64 (65.62%), Query Frame = 0

Query: 2  KSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTRT 61
          K  WPELVGK G+ A+ TIEREN  V A+++  G   I +F C+RV+V++    G+V   
Sbjct: 23 KYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLG-SNGQVADA 82

Query: 62 PIIG 66
          P+IG
Sbjct: 83 PMIG 85

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P823813.0e-1459.38Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1[more]
P240761.9e-1361.54Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1[more]
P198731.8e-1156.92Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1[more]
P802111.2e-1054.84Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1[more]
P869714.4e-1049.28Trypsin inhibitor OS=Fagopyrum tataricum OX=62330 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAA0049295.12.50e-3081.54inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa] >TYK1726... [more]
KAG6582359.11.69e-2876.92hypothetical protein SDJN03_22361, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6582360.14.73e-2875.38hypothetical protein SDJN03_22362, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_004134090.11.39e-2776.92glu S.griseus protease inhibitor [Cucumis sativus][more]
KAF7837521.15.53e-2778.46inhibitor of trypsin and hageman factor-like protein [Senna tora][more]
Match NameE-valueIdentityDescription
A0A5D3CZE21.21e-3081.54Inhibitor of trypsin and hageman factor-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A0A0L7956.75e-2876.92Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142960 PE=3 SV=1[more]
A0A067JP858.98e-2675.38Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_21815 PE=3 SV=1[more]
A0A4Y7J9L19.23e-2669.23Uncharacterized protein OS=Papaver somniferum OX=3469 GN=C5167_005120 PE=3 SV=1[more]
A0A7N2LXD55.34e-2576.92Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G43580.12.2e-1253.85Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT5G43570.12.4e-1153.23Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38870.13.5e-1050.00Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT3G46860.16.6e-0946.88Serine protease inhibitor, potato inhibitor I-type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRINTSPR00292POTATOINHBTRcoord: 4..17
score: 52.17
coord: 41..51
score: 52.57
coord: 29..40
score: 30.43
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 2..65
e-value: 1.5E-23
score: 83.3
IPR000864Proteinase inhibitor I13, potato inhibitor IPANTHERPTHR33091PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 1..65
IPR000864Proteinase inhibitor I13, potato inhibitor IPROSITEPS00285POTATO_INHIBITORcoord: 5..16
NoneNo IPR availableGENE3D3.30.10.10Trypsin Inhibitor V, subunit Acoord: 1..65
e-value: 2.2E-26
score: 94.0
NoneNo IPR availablePANTHERPTHR33091:SF41SERINE PROTEASE INHIBITOR, POTATO INHIBITOR I-TYPE FAMILY PROTEIN-RELATEDcoord: 1..65
IPR036354Proteinase inhibitor I13, potato inhibitor I superfamilySUPERFAMILY54654CI-2 family of serine protease inhibitorscoord: 1..65

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MC05g0054MC05g0054gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC05g0054.1-cdsMC05g0054.1-cds-MC05:407499..407693CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MC05g0054.1MC05g0054.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009611 response to wounding
biological_process GO:0010951 negative regulation of endopeptidase activity
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity