MC05g0054 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0054
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Inhibitor of trypsin and hageman factor-like
Location	MC05: 407499 .. 407693 (-)
RNA-Seq Expression	MC05g0054
Synteny	MC05g0054

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAAAGAGTTCGTGGCCAGAGTTGGTGGGCAAATTGGGAAAGCACGCAGAGAAAACCATCGAGAGGGAGAATCGTTTGGTTGATGCAGTGATTGTTGGCGAAGGCTCAAGTGTGATTCTTGACTTCAGGTGCGACAGGGTGTGGGTTTGGGTGAACGAGAAAACTGGTAGAGTTACTAGGACTCCTATTATTGGT

mRNA sequence

GGAAAGAGTTCGTGGCCAGAGTTGGTGGGCAAATTGGGAAAGCACGCAGAGAAAACCATCGAGAGGGAGAATCGTTTGGTTGATGCAGTGATTGTTGGCGAAGGCTCAAGTGTGATTCTTGACTTCAGGTGCGACAGGGTGTGGGTTTGGGTGAACGAGAAAACTGGTAGAGTTACTAGGACTCCTATTATTGGT

Coding sequence (CDS)

GGAAAGAGTTCGTGGCCAGAGTTGGTGGGCAAATTGGGAAAGCACGCAGAGAAAACCATCGAGAGGGAGAATCGTTTGGTTGATGCAGTGATTGTTGGCGAAGGCTCAAGTGTGATTCTTGACTTCAGGTGCGACAGGGTGTGGGTTTGGGTGAACGAGAAAACTGGTAGAGTTACTAGGACTCCTATTATTGGT

Protein sequence

GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTRTPIIG

Homology

[Close]

BLAST of MC05g0054 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.0e-14
Identity = 38/64 (59.38%), Postives = 47/64 (73.44%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GK++WPELVGK G  A  T+ERENR V A+++ EGS++  DFRCDRVWV VN+  G VT 
Sbjct: 6  GKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDH-GVVTS 65

Query: 61 TPII 65
           P I
Sbjct: 66 VPHI 68

[Close]

BLAST of MC05g0054 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.9e-13
Identity = 40/65 (61.54%), Postives = 43/65 (66.15%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GK SWP+LVG  G  A+  IEREN  V AVIV  GS V  DFRCDRV VWV E+ G V R
Sbjct: 5  GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTER-GIVAR 64

Query: 61 TPIIG 66
           P IG
Sbjct: 65 PPAIG 68

[Close]

BLAST of MC05g0054 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.8e-11
Identity = 37/65 (56.92%), Postives = 43/65 (66.15%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWP LVG  G  A+  IER+N  V AVI+ EG+ V  DFRC+RV +WVN K G V  
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVN-KRGLVVS 64

Query: 61 TPIIG 66
           P IG
Sbjct: 65 PPRIG 68

[Close]

BLAST of MC05g0054 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.2e-10
Identity = 34/62 (54.84%), Postives = 39/62 (62.90%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GK  WPELVG+ G  A   IEREN  V +++  E S    DFRCDRVWV V + TG V R
Sbjct: 6  GKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWV-VVDSTGVVVR 65

Query: 61 TP 63
          TP
Sbjct: 66 TP 66

[Close]

BLAST of MC05g0054 vs. ExPASy Swiss-Prot
Match: P86971 (Trypsin inhibitor OS=Fagopyrum tataricum OX=62330 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 4.4e-10
Identity = 34/69 (49.28%), Postives = 43/69 (62.32%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSV----ILDFRCDRVWVWVNEKTG 60
          GK SWPELVG  GK A  TI++EN  V AV+    +++      DFRCDRV V +N   G
Sbjct: 18 GKQSWPELVGTKGKTAAATIDKENTHVTAVLCPPLTTLAACRTFDFRCDRVRVLINRIGG 77

Query: 61 RVTRTPIIG 66
           VT+TP +G
Sbjct: 78 VVTKTPTVG 86

[Close]

BLAST of MC05g0054 vs. NCBI nr
Match: KAA0049295.1 (inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa] >TYK17263.1 inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa])

HSP 1 Score: 111 bits (277), Expect = 2.50e-30
Identity = 53/65 (81.54%), Postives = 55/65 (84.62%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVGK GK AEKTIEREN LVDA+IV EGS VI DFRCDRVWVWV+ KT  VTR
Sbjct: 7  GKSSWPELVGKAGKEAEKTIERENPLVDAIIVDEGSVVIQDFRCDRVWVWVDPKTAIVTR 66

Query: 61 TPIIG 65
          TP IG
Sbjct: 67 TPFIG 71

[Close]

BLAST of MC05g0054 vs. NCBI nr
Match: KAG6582359.1 (hypothetical protein SDJN03_22361, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 106 bits (265), Expect = 1.69e-28
Identity = 50/65 (76.92%), Postives = 54/65 (83.08%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVGK GK AE+ IE+EN LVDA++V EGS VI DFRCDRVWVWV  KTG VTR
Sbjct: 7  GKSSWPELVGKDGKEAERIIEKENPLVDAIVVDEGSVVIQDFRCDRVWVWVTVKTGIVTR 66

Query: 61 TPIIG 65
          TP IG
Sbjct: 67 TPFIG 71

[Close]

BLAST of MC05g0054 vs. NCBI nr
Match: KAG6582360.1 (hypothetical protein SDJN03_22362, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 105 bits (262), Expect = 4.73e-28
Identity = 49/65 (75.38%), Postives = 55/65 (84.62%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVGK+GK AEK IE+EN LVDA+IV EGS+V  DFRCDRVWVWV+  T  VTR
Sbjct: 6  GKSSWPELVGKVGKDAEKIIEKENPLVDAIIVDEGSNVPFDFRCDRVWVWVSPTTKIVTR 65

Query: 61 TPIIG 65
          TP+IG
Sbjct: 66 TPVIG 70

[Close]

BLAST of MC05g0054 vs. NCBI nr
Match: XP_004134090.1 (glu S.griseus protease inhibitor [Cucumis sativus])

HSP 1 Score: 104 bits (259), Expect = 1.39e-27
Identity = 50/65 (76.92%), Postives = 52/65 (80.00%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVGK GK AEK IE+EN  VDAVIV EGS V  DFRCDRVWVWV+ KT  VTR
Sbjct: 7  GKSSWPELVGKAGKEAEKIIEKENPSVDAVIVDEGSVVTQDFRCDRVWVWVDSKTSIVTR 66

Query: 61 TPIIG 65
          TP IG
Sbjct: 67 TPFIG 71

[Close]

BLAST of MC05g0054 vs. NCBI nr
Match: KAF7837521.1 (inhibitor of trypsin and hageman factor-like protein [Senna tora])

HSP 1 Score: 102 bits (255), Expect = 5.53e-27
Identity = 51/65 (78.46%), Postives = 53/65 (81.54%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVG  GK AE TIEREN LVDA+IV EGSSVI DFRCDRVWVWVN   G VT+
Sbjct: 7  GKSSWPELVGVKGKTAEATIERENSLVDAIIVDEGSSVITDFRCDRVWVWVNSD-GYVTK 66

Query: 61 TPIIG 65
           PIIG
Sbjct: 67 VPIIG 70

[Close]

BLAST of MC05g0054 vs. ExPASy TrEMBL
Match: A0A5D3CZE2 (Inhibitor of trypsin and hageman factor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001160 PE=3 SV=1)

HSP 1 Score: 111 bits (277), Expect = 1.21e-30
Identity = 53/65 (81.54%), Postives = 55/65 (84.62%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVGK GK AEKTIEREN LVDA+IV EGS VI DFRCDRVWVWV+ KT  VTR
Sbjct: 7  GKSSWPELVGKAGKEAEKTIERENPLVDAIIVDEGSVVIQDFRCDRVWVWVDPKTAIVTR 66

Query: 61 TPIIG 65
          TP IG
Sbjct: 67 TPFIG 71

[Close]

BLAST of MC05g0054 vs. ExPASy TrEMBL
Match: A0A0A0L795 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142960 PE=3 SV=1)

HSP 1 Score: 104 bits (259), Expect = 6.75e-28
Identity = 50/65 (76.92%), Postives = 52/65 (80.00%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVGK GK AEK IE+EN  VDAVIV EGS V  DFRCDRVWVWV+ KT  VTR
Sbjct: 7  GKSSWPELVGKAGKEAEKIIEKENPSVDAVIVDEGSVVTQDFRCDRVWVWVDSKTSIVTR 66

Query: 61 TPIIG 65
          TP IG
Sbjct: 67 TPFIG 71

[Close]

BLAST of MC05g0054 vs. ExPASy TrEMBL
Match: A0A067JP85 (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_21815 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 8.98e-26
Identity = 49/65 (75.38%), Postives = 53/65 (81.54%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPEL+G  GK A  TIE+EN  VDA++V EGSSVILDFRCDRVWVWVNE  G VTR
Sbjct: 7  GKSSWPELLGANGKAAAATIEKENGNVDAILVKEGSSVILDFRCDRVWVWVNEN-GVVTR 66

Query: 61 TPIIG 65
           PIIG
Sbjct: 67 VPIIG 70

[Close]

BLAST of MC05g0054 vs. ExPASy TrEMBL
Match: A0A4Y7J9L1 (Uncharacterized protein OS=Papaver somniferum OX=3469 GN=C5167_005120 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 9.23e-26
Identity = 45/65 (69.23%), Postives = 55/65 (84.62%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GK+SWPELVG LGK AEK IE +NR V+A+++ EGS+V LDFRCDRVWVW+NE  G+V R
Sbjct: 8  GKNSWPELVGCLGKTAEKKIEYQNRNVNAILIPEGSAVTLDFRCDRVWVWINE-AGKVVR 67

Query: 61 TPIIG 65
          TP+IG
Sbjct: 68 TPVIG 71

[Close]

BLAST of MC05g0054 vs. ExPASy TrEMBL
Match: A0A7N2LXD5 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 5.34e-25
Identity = 50/65 (76.92%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPELVG  G  AE TIEREN  VDAVIV EGSSVILDFRCDRVWVWV +K G V R
Sbjct: 8  GKSSWPELVGVQGTVAEATIERENSYVDAVIVPEGSSVILDFRCDRVWVWV-DKNGIVYR 67

Query: 61 TPIIG 65
           P IG
Sbjct: 68 VPTIG 71

[Close]

BLAST of MC05g0054 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 68.6 bits (166), Expect = 2.2e-12
Identity = 35/65 (53.85%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 1  GKSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTR 60
          GKSSWPEL+G  G+ A++ IEREN  + AVI+ +G+ V   F C RV+VWVN+  G V +
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVND-CGIVVQ 95

Query: 61 TPIIG 66
           PIIG
Sbjct: 96 IPIIG 99

[Close]

BLAST of MC05g0054 vs. TAIR 10
Match: AT5G43570.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 65.1 bits (157), Expect = 2.4e-11
Identity = 33/62 (53.23%), Postives = 44/62 (70.97%), Query Frame = 0


Query: 2  KSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTRT 61
          KSSWPELVG+ G+  ++ I+REN  V A I+ E + V+    CDRV+V VN++ G VTRT
Sbjct: 14 KSSWPELVGRRGEEVKEIIDRENTKVTAKIISENAVVLAVVICDRVYVRVNDQ-GIVTRT 73

Query: 62 PI 64
          PI
Sbjct: 74 PI 74

[Close]

BLAST of MC05g0054 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 61.2 bits (147), Expect = 3.5e-10
Identity = 32/64 (50.00%), Postives = 41/64 (64.06%), Query Frame = 0


Query: 2  KSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTRT 61
          K+SWPEL G  G +A   IEREN  V+A ++ +GS V  DFRCDRV V+V +    V +T
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFV-DGNRIVVKT 67

Query: 62 PIIG 66
          P  G
Sbjct: 68 PKSG 70

[Close]

BLAST of MC05g0054 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 57.0 bits (136), Expect = 6.6e-09
Identity = 30/64 (46.88%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 2  KSSWPELVGKLGKHAEKTIERENRLVDAVIVGEGSSVILDFRCDRVWVWVNEKTGRVTRT 61
          K  WPELVGK G+ A+ TIEREN  V A+++  G   I +F C+RV+V++    G+V   
Sbjct: 23 KYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLG-SNGQVADA 82

Query: 62 PIIG 66
          P+IG
Sbjct: 83 PMIG 85

The following BLAST results are available for this feature:

BLAST of MC05g0054 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P82381	3.0e-14	59.38	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
P24076	1.9e-13	61.54	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P19873	1.8e-11	56.92	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P80211	1.2e-10	54.84	Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1	[more]
P86971	4.4e-10	49.28	Trypsin inhibitor OS=Fagopyrum tataricum OX=62330 PE=1 SV=1	[more]

BLAST of MC05g0054 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0049295.1	2.50e-30	81.54	inhibitor of trypsin and hageman factor-like [Cucumis melo var. makuwa] >TYK1726...	[more]
KAG6582359.1	1.69e-28	76.92	hypothetical protein SDJN03_22361, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6582360.1	4.73e-28	75.38	hypothetical protein SDJN03_22362, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_004134090.1	1.39e-27	76.92	glu S.griseus protease inhibitor [Cucumis sativus]	[more]
KAF7837521.1	5.53e-27	78.46	inhibitor of trypsin and hageman factor-like protein [Senna tora]	[more]

BLAST of MC05g0054 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CZE2	1.21e-30	81.54	Inhibitor of trypsin and hageman factor-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A0A0L795	6.75e-28	76.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142960 PE=3 SV=1	[more]
A0A067JP85	8.98e-26	75.38	Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_21815 PE=3 SV=1	[more]
A0A4Y7J9L1	9.23e-26	69.23	Uncharacterized protein OS=Papaver somniferum OX=3469 GN=C5167_005120 PE=3 SV=1	[more]
A0A7N2LXD5	5.34e-25	76.92	Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]

BLAST of MC05g0054 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43580.1	2.2e-12	53.85	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43570.1	2.4e-11	53.23	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38870.1	3.5e-10	50.00	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	6.6e-09	46.88	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 4..17 score: 52.17 coord: 41..51 score: 52.57 coord: 29..40 score: 30.43
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 2..65 e-value: 1.5E-23 score: 83.3
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 1..65
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 5..16
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..65 e-value: 2.2E-26 score: 94.0
None	No IPR available	PANTHER	PTHR33091:SF41	SERINE PROTEASE INHIBITOR, POTATO INHIBITOR I-TYPE FAMILY PROTEIN-RELATED	coord: 1..65
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 1..65

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0054.1	MC05g0054.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity