MC04g0818.1 (mRNA) Bitter gourd (Dali-11) v1

Overview
NameMC04g0818.1
TypemRNA
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDNA-directed RNA polymerase subunit alpha
LocationMC04: 12415271 .. 12415396 (-)
Sequence length126
RNA-Seq ExpressionMC04g0818.1
SyntenyMC04g0818.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACACAATAAACAGAAAGTTACTTTACCCCTTTTTACTTTTCATGAGAAATTGGCCAAACTAAGGAAAAAGAAAAAAGAAATAGCATTAAAATATATTTTTATTGACCAATCAGAATTGCCTCCC

mRNA sequence

AAACACAATAAACAGAAAGTTACTTTACCCCTTTTTACTTTTCATGAGAAATTGGCCAAACTAAGGAAAAAGAAAAAAGAAATAGCATTAAAATATATTTTTATTGACCAATCAGAATTGCCTCCC

Coding sequence (CDS)

AAACACAATAAACAGAAAGTTACTTTACCCCTTTTTACTTTTCATGAGAAATTGGCCAAACTAAGGAAAAAGAAAAAAGAAATAGCATTAAAATATATTTTTATTGACCAATCAGAATTGCCTCCC

Protein sequence

KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP
Homology
BLAST of MC04g0818.1 vs. ExPASy Swiss-Prot
Match: Q4VZK3 (DNA-directed RNA polymerase subunit alpha OS=Cucumis sativus OX=3659 GN=rpoA PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 3.0e-15
Identity = 40/42 (95.24%), Postives = 42/42 (100.00%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 43
           K+NK+KVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP
Sbjct: 242 KNNKKKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 283

BLAST of MC04g0818.1 vs. ExPASy Swiss-Prot
Match: Q09G14 (DNA-directed RNA polymerase subunit alpha OS=Platanus occidentalis OX=4403 GN=rpoA PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 9.8e-11
Identity = 31/40 (77.50%), Postives = 36/40 (90.00%), Query Frame = 0

Query: 3   NKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 43
           N+  VTLP FTFH++LAK+RK +KEIALKYIFIDQSELPP
Sbjct: 242 NQNGVTLPFFTFHDRLAKIRKNEKEIALKYIFIDQSELPP 281

BLAST of MC04g0818.1 vs. ExPASy Swiss-Prot
Match: Q49KW6 (DNA-directed RNA polymerase subunit alpha OS=Eucalyptus globulus subsp. globulus OX=71271 GN=rpoA PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.7e-10
Identity = 32/42 (76.19%), Postives = 37/42 (88.10%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 43
           ++N+ KVTLP FTFH++LAKLRK KKEIALK IFIDQ ELPP
Sbjct: 240 ENNQYKVTLPFFTFHDRLAKLRKTKKEIALKSIFIDQFELPP 281

BLAST of MC04g0818.1 vs. ExPASy Swiss-Prot
Match: Q2L938 (DNA-directed RNA polymerase subunit alpha OS=Gossypium hirsutum OX=3635 GN=rpoA PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.7e-10
Identity = 31/42 (73.81%), Postives = 36/42 (85.71%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 43
           ++N+  VTLP F FH++L KL KKKKEIALKYIFIDQSELPP
Sbjct: 242 ENNQHDVTLPFFPFHDRLVKLTKKKKEIALKYIFIDQSELPP 283

BLAST of MC04g0818.1 vs. ExPASy Swiss-Prot
Match: Q09ME5 (DNA-directed RNA polymerase subunit alpha OS=Citrus sinensis OX=2711 GN=rpoA PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 4.9e-10
Identity = 31/42 (73.81%), Postives = 37/42 (88.10%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 43
           ++N+ KVTLP FTFH++LAKL KKKKEIALK IFIDQSE+ P
Sbjct: 241 ENNQYKVTLPFFTFHDRLAKLTKKKKEIALKSIFIDQSEMSP 282

BLAST of MC04g0818.1 vs. NCBI nr
Match: YP_009752867.1 (RNA polymerase subunit alpha [Baijiania yunnanensis] >QIT05939.1 RNA polymerase subunit alpha [Baijiania yunnanensis])

HSP 1 Score: 82.4 bits (202), Expect = 4.21e-17
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 42
           K+NKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP
Sbjct: 240 KNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 281

BLAST of MC04g0818.1 vs. NCBI nr
Match: YP_009752107.1 (RNA polymerase subunit alpha [Dendrosicyos socotranus] >QIT05517.1 RNA polymerase subunit alpha [Dendrosicyos socotranus])

HSP 1 Score: 82.4 bits (202), Expect = 4.21e-17
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 42
           K+NKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP
Sbjct: 240 KNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 281

BLAST of MC04g0818.1 vs. NCBI nr
Match: YP_009456093.1 (RNA polymerase alpha subunit [Momordica charantia] >AUJ21860.1 RNA polymerase alpha subunit [Momordica charantia])

HSP 1 Score: 82.4 bits (202), Expect = 4.21e-17
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 42
           K+NKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP
Sbjct: 240 KNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 281

BLAST of MC04g0818.1 vs. NCBI nr
Match: ALO21933.1 (RpoA [Cucurbita cordata] >ALO22018.1 RpoA [Cucurbita digitata])

HSP 1 Score: 82.4 bits (202), Expect = 4.21e-17
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 42
           K+NKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP
Sbjct: 240 KNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 281

BLAST of MC04g0818.1 vs. NCBI nr
Match: YP_009752022.1 (RNA polymerase subunit alpha [Cionosicys macranthus] >QIT05263.1 RNA polymerase subunit alpha [Cionosicys macranthus])

HSP 1 Score: 82.4 bits (202), Expect = 4.21e-17
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 42
           K+NKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP
Sbjct: 240 KNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 281

BLAST of MC04g0818.1 vs. ExPASy TrEMBL
Match: A0A6H0ES49 (DNA-directed RNA polymerase subunit alpha OS=Indofevillea khasiana OX=383665 GN=rpoA PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.04e-17
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 42
           K+NKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP
Sbjct: 240 KNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 281

BLAST of MC04g0818.1 vs. ExPASy TrEMBL
Match: A0A0S2IF96 (DNA-directed RNA polymerase subunit alpha OS=Cucurbita moschata OX=3662 GN=rpoA PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.04e-17
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 42
           K+NKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP
Sbjct: 240 KNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 281

BLAST of MC04g0818.1 vs. ExPASy TrEMBL
Match: A0A514YKX0 (DNA-directed RNA polymerase subunit alpha OS=Siraitia grosvenorii OX=190515 GN=rpoA PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.04e-17
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 42
           K+NKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP
Sbjct: 240 KNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 281

BLAST of MC04g0818.1 vs. ExPASy TrEMBL
Match: A0A6H0EP10 (DNA-directed RNA polymerase subunit alpha OS=Luffa aegyptiaca OX=3670 GN=rpoA PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.04e-17
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 42
           K+NKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP
Sbjct: 240 KNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 281

BLAST of MC04g0818.1 vs. ExPASy TrEMBL
Match: A0A0S2IDS0 (DNA-directed RNA polymerase subunit alpha OS=Cucurbita argyrosperma OX=34294 GN=rpoA PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.04e-17
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 42
           K+NKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP
Sbjct: 240 KNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 281

BLAST of MC04g0818.1 vs. TAIR 10
Match: ATCG00740.1 (RNA polymerase subunit alpha )

HSP 1 Score: 54.7 bits (130), Expect = 2.1e-08
Identity = 27/44 (61.36%), Postives = 34/44 (77.27%), Query Frame = 0

Query: 1   KHNKQKVTLPLFTFHEKLAKLRKKK--KEIALKYIFIDQSELPP 43
           ++N+ +VTLP F FH +L  LRKKK  KE+A +YIFIDQ ELPP
Sbjct: 240 ENNQHQVTLPFFPFHNRLVNLRKKKKTKELAFQYIFIDQLELPP 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q4VZK33.0e-1595.24DNA-directed RNA polymerase subunit alpha OS=Cucumis sativus OX=3659 GN=rpoA PE=... [more]
Q09G149.8e-1177.50DNA-directed RNA polymerase subunit alpha OS=Platanus occidentalis OX=4403 GN=rp... [more]
Q49KW61.7e-1076.19DNA-directed RNA polymerase subunit alpha OS=Eucalyptus globulus subsp. globulus... [more]
Q2L9381.7e-1073.81DNA-directed RNA polymerase subunit alpha OS=Gossypium hirsutum OX=3635 GN=rpoA ... [more]
Q09ME54.9e-1073.81DNA-directed RNA polymerase subunit alpha OS=Citrus sinensis OX=2711 GN=rpoA PE=... [more]
Match NameE-valueIdentityDescription
YP_009752867.14.21e-1797.62RNA polymerase subunit alpha [Baijiania yunnanensis] >QIT05939.1 RNA polymerase ... [more]
YP_009752107.14.21e-1797.62RNA polymerase subunit alpha [Dendrosicyos socotranus] >QIT05517.1 RNA polymeras... [more]
YP_009456093.14.21e-1797.62RNA polymerase alpha subunit [Momordica charantia] >AUJ21860.1 RNA polymerase al... [more]
ALO21933.14.21e-1797.62RpoA [Cucurbita cordata] >ALO22018.1 RpoA [Cucurbita digitata][more]
YP_009752022.14.21e-1797.62RNA polymerase subunit alpha [Cionosicys macranthus] >QIT05263.1 RNA polymerase ... [more]
Match NameE-valueIdentityDescription
A0A6H0ES492.04e-1797.62DNA-directed RNA polymerase subunit alpha OS=Indofevillea khasiana OX=383665 GN=... [more]
A0A0S2IF962.04e-1797.62DNA-directed RNA polymerase subunit alpha OS=Cucurbita moschata OX=3662 GN=rpoA ... [more]
A0A514YKX02.04e-1797.62DNA-directed RNA polymerase subunit alpha OS=Siraitia grosvenorii OX=190515 GN=r... [more]
A0A6H0EP102.04e-1797.62DNA-directed RNA polymerase subunit alpha OS=Luffa aegyptiaca OX=3670 GN=rpoA PE... [more]
A0A0S2IDS02.04e-1797.62DNA-directed RNA polymerase subunit alpha OS=Cucurbita argyrosperma OX=34294 GN=... [more]
Match NameE-valueIdentityDescription
ATCG00740.12.1e-0861.36RNA polymerase subunit alpha [more]
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MC04g0818MC04g0818gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MC04g0818.1-cdsMC04g0818.1-cds-MC04:12415271..12415396CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MC04g0818.1MC04g0818.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0009507 chloroplast
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0046983 protein dimerization activity