MC00g0174.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC00g0174.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RNase H domain-containing protein
Location	scaffold18: 221019 .. 221336 (-)
Sequence length	318
RNA-Seq Expression	MC00g0174.1
Synteny	MC00g0174.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGTCTATTTCAGATTCACAAGGGAGAGGGAGTTGGATTCTTACCTATATGAAAGAAATTGAGGCTAATAACTCGTCTTTGTTGTCTGATGGTAATGCTCGTGAAACCAATCGTAGTAGTAGTGTTTGGAATCCCCCTCCTCCTGGGTGGGTTAAGATAAACAGGGATGTGGCATGCAAGAAAAGCAATTCCAACTCTGGATTAGGGGCAATTTGTAGAGATGAAGACGGTGGAATTTTTTCTGCAATGGCAAAGAATACAGGAGTGTATCACGAACCTTTAATAGCGGAATTACTGGCAATTCTTGAGGGACTGAGG

mRNA sequence

CAGTCTATTTCAGATTCACAAGGGAGAGGGAGTTGGATTCTTACCTATATGAAAGAAATTGAGGCTAATAACTCGTCTTTGTTGTCTGATGGTAATGCTCGTGAAACCAATCGTAGTAGTAGTGTTTGGAATCCCCCTCCTCCTGGGTGGGTTAAGATAAACAGGGATGTGGCATGCAAGAAAAGCAATTCCAACTCTGGATTAGGGGCAATTTGTAGAGATGAAGACGGTGGAATTTTTTCTGCAATGGCAAAGAATACAGGAGTGTATCACGAACCTTTAATAGCGGAATTACTGGCAATTCTTGAGGGACTGAGG

Coding sequence (CDS)

CAGTCTATTTCAGATTCACAAGGGAGAGGGAGTTGGATTCTTACCTATATGAAAGAAATTGAGGCTAATAACTCGTCTTTGTTGTCTGATGGTAATGCTCGTGAAACCAATCGTAGTAGTAGTGTTTGGAATCCCCCTCCTCCTGGGTGGGTTAAGATAAACAGGGATGTGGCATGCAAGAAAAGCAATTCCAACTCTGGATTAGGGGCAATTTGTAGAGATGAAGACGGTGGAATTTTTTCTGCAATGGCAAAGAATACAGGAGTGTATCACGAACCTTTAATAGCGGAATTACTGGCAATTCTTGAGGGACTGAGG

Protein sequence

QSISDSQGRGSWILTYMKEIEANNSSLLSDGNARETNRSSSVWNPPPPGWVKINRDVACKKSNSNSGLGAICRDEDGGIFSAMAKNTGVYHEPLIAELLAILEGLR

Homology

[Close]

BLAST of MC00g0174.1 vs. NCBI nr
Match: XP_022145060.1 (uncharacterized protein LOC111014578 [Momordica charantia])

HSP 1 Score: 85.5 bits (210), Expect = 7.57e-18
Identity = 42/109 (38.53%), Postives = 65/109 (59.63%), Query Frame = 0


Query: 1   QSISDSQGRGSWILTYMKEIEANNSSLLSDGNARETNRSS-----SVWNPPPPGWVKINR 60
           + I D++ R  WILTY+++ +  +   L D   +E   S+     + W+PPP GW+KIN 
Sbjct: 106 RQIPDAKIRSDWILTYVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINV 165

Query: 61  DVACKKSNSNSGLGAICRDEDGGIFSAMAKNTGVYHEPLIAELLAILEG 104
           D ACKK    +G+G +CR+E G I +A +      H+PL+AE LA+ +G
Sbjct: 166 DAACKKHQFRTGIGIVCRNEKGQILAAAS------HDPLMAESLALFDG 208

[Close]

BLAST of MC00g0174.1 vs. NCBI nr
Match: XP_022154440.1 (uncharacterized protein LOC111021711 [Momordica charantia])

HSP 1 Score: 82.4 bits (202), Expect = 3.25e-16
Identity = 36/74 (48.65%), Postives = 49/74 (66.22%), Query Frame = 0


Query: 32  NARETNRSSSVWNPPPPGWVKINRDVACKKSNSNSGLGAICRDEDGGIFSAMAKNTGVYH 91
           N+ +  +  + W PP P WVKIN D ACK SNS +G+G +C+++ G I  AM +   V++
Sbjct: 117 NSNKAGKDVNYWAPPSPDWVKINVDTACKLSNSRTGIGVVCQNDKGEIIVAMIRKLDVFY 176

Query: 92  EPLIAELLAILEGL 105
           EPL  E LAILEGL
Sbjct: 177 EPLQVETLAILEGL 190

[Close]

BLAST of MC00g0174.1 vs. NCBI nr
Match: XP_022142956.1 (uncharacterized protein LOC111012950 [Momordica charantia])

HSP 1 Score: 73.2 bits (178), Expect = 1.78e-13
Identity = 31/58 (53.45%), Postives = 42/58 (72.41%), Query Frame = 0


Query: 49  GWVKINRDVACKKSNSNSGLGAICRDEDGGIFSAMAKNTGVYHEPLIAELLAILEGLR 106
           GW+KIN DVACKK    + +G +CR+E G I +A ++    YH+PL+AE LA+LEGLR
Sbjct: 31  GWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLR 88

[Close]

BLAST of MC00g0174.1 vs. NCBI nr
Match: KAE8659133.1 (Mannose-P-dolichol utilization defect 1 protein [Hibiscus syriacus])

HSP 1 Score: 67.8 bits (164), Expect = 1.66e-10
Identity = 38/102 (37.25%), Postives = 56/102 (54.90%), Query Frame = 0


Query: 11  SWILTYMKEIEANNSSLLSD-------GNARETNRSSSVWNPPPPGWVKINRDVACKKSN 70
           SW  T +K++  +  SL+ D       GN+ + N  SS W+PPP G++K+N D A  K  
Sbjct: 601 SWFETSVKDVHFHFDSLMGDLKLADQLGNSHKKNLPSSKWSPPPEGFLKVNVDGAMVKDW 660

Query: 71  SNSGLGAICRDEDGGIFSAMAKNTGVYHEPLIAELLAILEGL 105
              G+G + RD  G +  + ++  G    PL+AELLAI  GL
Sbjct: 661 DKGGIGGLIRDGSGLVLGSFSEKVGG-GPPLLAELLAIKRGL 701

[Close]

BLAST of MC00g0174.1 vs. NCBI nr
Match: PRQ37402.1 (putative ribonuclease H-like domain, reverse transcriptase zinc-binding domain-containing protein [Rosa chinensis])

HSP 1 Score: 65.9 bits (159), Expect = 7.68e-10
Identity = 32/75 (42.67%), Postives = 42/75 (56.00%), Query Frame = 0


Query: 32  NARETNRSSSVWNPPPPGWVKINRDVACKKSNSNSGLGAICRDEDGGIFSAMAKNTGVYH 91
           N R  N+    W  PPP W K+N D AC+ +  NSGLGA+ R+E G +  A+A       
Sbjct: 412 NVRSKNQLEVKWERPPPQWCKLNFDGACEANGGNSGLGAVIRNELGELVGALAVPFAATL 471

Query: 92  EPLIAELLAILEGLR 106
           +P   E LA+ EGLR
Sbjct: 472 KPSAVEALALQEGLR 486

[Close]

BLAST of MC00g0174.1 vs. ExPASy TrEMBL
Match: A0A6J1CTE3 (uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014578 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 3.66e-18
Identity = 42/109 (38.53%), Postives = 65/109 (59.63%), Query Frame = 0


Query: 1   QSISDSQGRGSWILTYMKEIEANNSSLLSDGNARETNRSS-----SVWNPPPPGWVKINR 60
           + I D++ R  WILTY+++ +  +   L D   +E   S+     + W+PPP GW+KIN 
Sbjct: 106 RQIPDAKIRSDWILTYVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINV 165

Query: 61  DVACKKSNSNSGLGAICRDEDGGIFSAMAKNTGVYHEPLIAELLAILEG 104
           D ACKK    +G+G +CR+E G I +A +      H+PL+AE LA+ +G
Sbjct: 166 DAACKKHQFRTGIGIVCRNEKGQILAAAS------HDPLMAESLALFDG 208

[Close]

BLAST of MC00g0174.1 vs. ExPASy TrEMBL
Match: A0A6J1DJL6 (uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021711 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.57e-16
Identity = 36/74 (48.65%), Postives = 49/74 (66.22%), Query Frame = 0


Query: 32  NARETNRSSSVWNPPPPGWVKINRDVACKKSNSNSGLGAICRDEDGGIFSAMAKNTGVYH 91
           N+ +  +  + W PP P WVKIN D ACK SNS +G+G +C+++ G I  AM +   V++
Sbjct: 117 NSNKAGKDVNYWAPPSPDWVKINVDTACKLSNSRTGIGVVCQNDKGEIIVAMIRKLDVFY 176

Query: 92  EPLIAELLAILEGL 105
           EPL  E LAILEGL
Sbjct: 177 EPLQVETLAILEGL 190

[Close]

BLAST of MC00g0174.1 vs. ExPASy TrEMBL
Match: A0A6J1CNR4 (uncharacterized protein LOC111012950 OS=Momordica charantia OX=3673 GN=LOC111012950 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 8.60e-14
Identity = 31/58 (53.45%), Postives = 42/58 (72.41%), Query Frame = 0


Query: 49  GWVKINRDVACKKSNSNSGLGAICRDEDGGIFSAMAKNTGVYHEPLIAELLAILEGLR 106
           GW+KIN DVACKK    + +G +CR+E G I +A ++    YH+PL+AE LA+LEGLR
Sbjct: 31  GWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLR 88

[Close]

BLAST of MC00g0174.1 vs. ExPASy TrEMBL
Match: A0A6A2WIS1 (Mannose-P-dolichol utilization defect 1 protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00116964pilonHSYRG00162 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 8.03e-11
Identity = 38/102 (37.25%), Postives = 56/102 (54.90%), Query Frame = 0


Query: 11  SWILTYMKEIEANNSSLLSD-------GNARETNRSSSVWNPPPPGWVKINRDVACKKSN 70
           SW  T +K++  +  SL+ D       GN+ + N  SS W+PPP G++K+N D A  K  
Sbjct: 601 SWFETSVKDVHFHFDSLMGDLKLADQLGNSHKKNLPSSKWSPPPEGFLKVNVDGAMVKDW 660

Query: 71  SNSGLGAICRDEDGGIFSAMAKNTGVYHEPLIAELLAILEGL 105
              G+G + RD  G +  + ++  G    PL+AELLAI  GL
Sbjct: 661 DKGGIGGLIRDGSGLVLGSFSEKVGG-GPPLLAELLAIKRGL 701

[Close]

BLAST of MC00g0174.1 vs. ExPASy TrEMBL
Match: A0A2P6QT88 (Putative ribonuclease H-like domain, reverse transcriptase zinc-binding domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0402201 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 3.72e-10
Identity = 32/75 (42.67%), Postives = 42/75 (56.00%), Query Frame = 0


Query: 32  NARETNRSSSVWNPPPPGWVKINRDVACKKSNSNSGLGAICRDEDGGIFSAMAKNTGVYH 91
           N R  N+    W  PPP W K+N D AC+ +  NSGLGA+ R+E G +  A+A       
Sbjct: 412 NVRSKNQLEVKWERPPPQWCKLNFDGACEANGGNSGLGAVIRNELGELVGALAVPFAATL 471

Query: 92  EPLIAELLAILEGLR 106
           +P   E LA+ EGLR
Sbjct: 472 KPSAVEALALQEGLR 486

The following BLAST results are available for this feature:

BLAST of MC00g0174.1 vs.


Match Name	E-value	Identity	Description

BLAST of MC00g0174.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145060.1	7.57e-18	38.53	uncharacterized protein LOC111014578 [Momordica charantia]	[more]
XP_022154440.1	3.25e-16	48.65	uncharacterized protein LOC111021711 [Momordica charantia]	[more]
XP_022142956.1	1.78e-13	53.45	uncharacterized protein LOC111012950 [Momordica charantia]	[more]
KAE8659133.1	1.66e-10	37.25	Mannose-P-dolichol utilization defect 1 protein [Hibiscus syriacus]	[more]
PRQ37402.1	7.68e-10	42.67	putative ribonuclease H-like domain, reverse transcriptase zinc-binding domain-c...	[more]

BLAST of MC00g0174.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTE3	3.66e-18	38.53	uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A6J1DJL6	1.57e-16	48.65	uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A6J1CNR4	8.60e-14	53.45	uncharacterized protein LOC111012950 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6A2WIS1	8.03e-11	37.25	Mannose-P-dolichol utilization defect 1 protein OS=Hibiscus syriacus OX=106335 G...	[more]
A0A2P6QT88	3.72e-10	42.67	Putative ribonuclease H-like domain, reverse transcriptase zinc-binding domain-c...	[more]

BLAST of MC00g0174.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 54..106 e-value: 2.2E-7 score: 30.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 27..44
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 27..48
None	No IPR available	PANTHER	PTHR47074	BNAC02G40300D PROTEIN	coord: 34..106
None	No IPR available	PANTHER	PTHR47074:SF37	PROTEIN, PUTATIVE-RELATED	coord: 34..106

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC00g0174	MC00g0174	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC00g0174.1-cds	MC00g0174.1-cds-scaffold18:221019..221336	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC00g0174.1	MC00g0174.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity