Lsi09G018790.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G018790.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	aspartic proteinase CDR1-like
Location	chr09: 27602174 .. 27604605 (-)
Sequence length	1323
RNA-Seq Expression	Lsi09G018790.1
Synteny	Lsi09G018790.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGTTAAAATATCTTCATAATCAAGTTGTGTTAGGTTAACAATAAAATGAAACCAGAATATTTTGTACTTCCCGTTTGATAATTAATCATTTGACTTCTTTTATTTCTTAAATTTTGCATGTTTTTCACACTTTCTCTACAATGTAGTTCATATTTTTTAAAGAACGATTTAAATTCTTAGCCAAATCTAAAAAATAAAAATCACTTTAGAAAAAAAAAATTGAATGAATTTTGCAAAACATTGTTAAAAAGTAGATGAGAAAATAAAGAAATTCATAGTTGAAAATTCGAAAATTATTATAAATGAAAAAATACCAAACTATTTACAAATATAGAAAACACGGATAGAAGTTTATCACAATCTATCGCTGATTGACAATGAAATTTTTCTATATTTATAAATATTTTGGCTCATTTTTCTATTTTTAAAAATAGTCCTTGAAAATTTATGTTAATAATAAGCTTACCGATACCAAGAACAAATCAAAATTAGGAGAAAAAAAAAAGAAAACGAACAAATAAACAATTTTATCCTTCAATTTCTAAATTTGAGTAATTATTAGTTACACCTAACCATGAATATTTATTTTCTATTTAAAAAAAAATATTTACAACACAGTCAGAATTTTATTGGACAATCACTTAAAATACACCAAAGTAGGTGGAGCCAATAACACTCAAATTTTTTCATCCAAAATTTGAAGTAAAAAAACCAATTATGGCACTGTTCAATTAATTAGATCGATCATAATTACCCAAAATCCCACCACCTCACTACCCATTTATCACATTATTACCATATCATTGTAACAAAACGAGTCCAATTAACCGAAACCAAACGTTTTTCAATTATCAATAATATCAAAATTAATAACAGTTACAAATTTACTTCCCTTCCATTCGTTTCCAATTTTTAATAACCTCTCAAATTTCTCTCTTCTATATATTTAATCAACCTCTCCATTCATTTCTTCATCACCAATTCATCAATGGCACCCATTTTCTCTCTTATTATTTTCTTAATCTCCTCCACCGCCGTCTCGGCTGCCGCTACAGGCCGTGACTATGGCTTCACCATCGAACTCATCCACCGTGACTCTCCCAAGTCCCCTATGTACAATCCATTGAAGACTCACTTCGACCGTCTCACTGACGCCCTCCGCCGTTCCATCAATCGTAACACGGTGGCGTTGACAACAGTCACGGCAGAGGCCCCTATTTACAACAATAGAGGCGAATACCTAATGAAAATATCCCTCGGAACACCGCCGTTTTCGATTATAGCTGTTGCTGACACAGGAAGCGACATCATTTGGACCCAATGCCAACCATGCCAAAATTGCTTCCAACAAAATGCACCAATGTTTAACCCGAGTAAATCGGTGACTTATAAAAAAGTGGCGTGCTCCTCATCGATTTGCTTGTTTGCAGGTGATAGTTCTTCATGTTCCTCTCAATCCTTTCAGTCTGACTGTTTGTACTCGATTACTTACGGTGATAGGTCCCACACCAAAGGAGATTTTGCCGTTGATACTGTTACTATGGGGTCCACCTCTGGTCGCCCCGTGGCGTTTCCTCGTACTGCGATTGGTTGTGGTCATGACAATGCTGGCACTTTCAGTGCTAATATTTCTGGCATTGTTGGACTCGGGCTAGGTCCGGCTTCGCTTGTCAAGCAGATGGGACCGGCCGCTGGCGGAAAATTCTCTTACTGTTTAACTCCGATTGGTAGCAGGAATATCGAATCCAACAAACTTAACTTTGGCTCTAATGCCGACGTCTCTGGCTCTGGAGTAGTCTCAACTCCTATTCATATTAGTGGTAAACAAATTATTACTACTAAACTCAAAAGCTTAAGTTGATTTTTTTTTATTGATGCAATCATTCATACTTGGGTGGGTGGGTGGATTTGTGTATATTTAGTTAATCTTTTGTTTGGATTTTTAACAGATACATTCAAAAGTTTCTACTCGCTCAATTTAGAAGCCGTGAGCGTTGGGGAGAAGAAATTTGATTTTCCCGTCTCTTCGGTATTAGGCGGAGGAGCAAACATCGTCATTGACTCTGGCACGACGCTTACTTTCCTCCCACCGGATTTATACAACGACTTCTCCACAGCAATTTCCAACGCGATAAACCTCCAACGCACGAACGACCCCGATCAAGTCTTGGATTGCTGCTTTGCGACCACCACCGACGACTACAAAACGCCACCCATCACGATGCACTTTGAAGGTGCCGATGTGCCCCTCTCCCAAGAAAATGTGTTCATTAGGGTGTCGAACGACGTTGTTTGCTTGGCCTTTAGCGCCGGCCAAGAAAATGGCATTATGTTATATGGCAATATTGCACAGGGCAACTTCTTGATTGGTTACGATATCAAGAACATGTTTGTTTCTTTCAAGCCCACGAATTGTGTTGCCATGTGA

mRNA sequence

ATGAGTCAGAATTTTATTGGACAATCACTTAAAATACACCAAAGCCGTGACTATGGCTTCACCATCGAACTCATCCACCGTGACTCTCCCAAGTCCCCTATGTACAATCCATTGAAGACTCACTTCGACCGTCTCACTGACGCCCTCCGCCGTTCCATCAATCGTAACACGGTGGCGTTGACAACAGTCACGGCAGAGGCCCCTATTTACAACAATAGAGGCGAATACCTAATGAAAATATCCCTCGGAACACCGCCGTTTTCGATTATAGCTGTTGCTGACACAGGAAGCGACATCATTTGGACCCAATGCCAACCATGCCAAAATTGCTTCCAACAAAATGCACCAATGTTTAACCCGAGTAAATCGGTGACTTATAAAAAAGTGGCGTGCTCCTCATCGATTTGCTTGTTTGCAGGTGATAGTTCTTCATGTTCCTCTCAATCCTTTCAGTCTGACTGTTTGTACTCGATTACTTACGGTGATAGGTCCCACACCAAAGGAGATTTTGCCGTTGATACTGTTACTATGGGGTCCACCTCTGGTCGCCCCGTGGCGTTTCCTCGTACTGCGATTGGTTGTGGTCATGACAATGCTGGCACTTTCAGTGCTAATATTTCTGGCATTGTTGGACTCGGGCTAGGTCCGGCTTCGCTTGTCAAGCAGATGGGACCGGCCGCTGGCGGAAAATTCTCTTACTGTTTAACTCCGATTGGTAGCAGGAATATCGAATCCAACAAACTTAACTTTGGCTCTAATGCCGACGTCTCTGGCTCTGGAGTAGTCTCAACTCCTATTCATATTAGTGGTAAACAAATTATTACTACTAAACTCAAAAGCTTAAATACATTCAAAAGTTTCTACTCGCTCAATTTAGAAGCCGTGAGCGTTGGGGAGAAGAAATTTGATTTTCCCGTCTCTTCGGTATTAGGCGGAGGAGCAAACATCGTCATTGACTCTGGCACGACGCTTACTTTCCTCCCACCGGATTTATACAACGACTTCTCCACAGCAATTTCCAACGCGATAAACCTCCAACGCACGAACGACCCCGATCAAGTCTTGGATTGCTGCTTTGCGACCACCACCGACGACTACAAAACGCCACCCATCACGATGCACTTTGAAGGTGCCGATGTGCCCCTCTCCCAAGAAAATGTGTTCATTAGGGTGTCGAACGACGTTGTTTGCTTGGCCTTTAGCGCCGGCCAAGAAAATGGCATTATGTTATATGGCAATATTGCACAGGGCAACTTCTTGATTGGTTACGATATCAAGAACATGTTTGTTTCTTTCAAGCCCACGAATTGTGTTGCCATGTGA

Coding sequence (CDS)

ATGAGTCAGAATTTTATTGGACAATCACTTAAAATACACCAAAGCCGTGACTATGGCTTCACCATCGAACTCATCCACCGTGACTCTCCCAAGTCCCCTATGTACAATCCATTGAAGACTCACTTCGACCGTCTCACTGACGCCCTCCGCCGTTCCATCAATCGTAACACGGTGGCGTTGACAACAGTCACGGCAGAGGCCCCTATTTACAACAATAGAGGCGAATACCTAATGAAAATATCCCTCGGAACACCGCCGTTTTCGATTATAGCTGTTGCTGACACAGGAAGCGACATCATTTGGACCCAATGCCAACCATGCCAAAATTGCTTCCAACAAAATGCACCAATGTTTAACCCGAGTAAATCGGTGACTTATAAAAAAGTGGCGTGCTCCTCATCGATTTGCTTGTTTGCAGGTGATAGTTCTTCATGTTCCTCTCAATCCTTTCAGTCTGACTGTTTGTACTCGATTACTTACGGTGATAGGTCCCACACCAAAGGAGATTTTGCCGTTGATACTGTTACTATGGGGTCCACCTCTGGTCGCCCCGTGGCGTTTCCTCGTACTGCGATTGGTTGTGGTCATGACAATGCTGGCACTTTCAGTGCTAATATTTCTGGCATTGTTGGACTCGGGCTAGGTCCGGCTTCGCTTGTCAAGCAGATGGGACCGGCCGCTGGCGGAAAATTCTCTTACTGTTTAACTCCGATTGGTAGCAGGAATATCGAATCCAACAAACTTAACTTTGGCTCTAATGCCGACGTCTCTGGCTCTGGAGTAGTCTCAACTCCTATTCATATTAGTGGTAAACAAATTATTACTACTAAACTCAAAAGCTTAAATACATTCAAAAGTTTCTACTCGCTCAATTTAGAAGCCGTGAGCGTTGGGGAGAAGAAATTTGATTTTCCCGTCTCTTCGGTATTAGGCGGAGGAGCAAACATCGTCATTGACTCTGGCACGACGCTTACTTTCCTCCCACCGGATTTATACAACGACTTCTCCACAGCAATTTCCAACGCGATAAACCTCCAACGCACGAACGACCCCGATCAAGTCTTGGATTGCTGCTTTGCGACCACCACCGACGACTACAAAACGCCACCCATCACGATGCACTTTGAAGGTGCCGATGTGCCCCTCTCCCAAGAAAATGTGTTCATTAGGGTGTCGAACGACGTTGTTTGCTTGGCCTTTAGCGCCGGCCAAGAAAATGGCATTATGTTATATGGCAATATTGCACAGGGCAACTTCTTGATTGGTTACGATATCAAGAACATGTTTGTTTCTTTCAAGCCCACGAATTGTGTTGCCATGTGA

Protein sequence

MSQNFIGQSLKIHQSRDYGFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYNNRGEYLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSSICLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGCGHDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSNADVSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPVSSVLGGGANIVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPITMHFEGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSFKPTNCVAM

Homology

[Close]

BLAST of Lsi09G018790.1 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 1.2e-114
Identity = 209/425 (49.18%), Postives = 283/425 (66.59%), Query Frame = 0


Query: 19  GFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINR---NTVALTTVTAEAPIYNNRGE 78
           GFT +LIHRDSPKSP YNP++T   RL +A+ RS+NR    T    T   +  + +N GE
Sbjct: 30  GFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRVFHFTEKDNTPQPQIDLTSNSGE 89

Query: 79  YLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSSI 138
           YLM +S+GTPPF I+A+ADTGSD++WTQC PC +C+ Q  P+F+P  S TYK V+CSSS 
Sbjct: 90  YLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCSSSQ 149

Query: 139 CLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGCG 198
           C    + +SCS+    + C YS++YGD S+TKG+ AVDT+T+GS+  RP+      IGCG
Sbjct: 150 CTALENQASCSTN--DNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGCG 209

Query: 199 HDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSNAD 258
           H+NAGTF+   SGIVGLG GP SL+KQ+G +  GKFSYCL P+ S+  +++K+NFG+NA 
Sbjct: 210 HNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYCLVPLTSKKDQTSKINFGTNAI 269

Query: 259 VSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPVSSVLGGGAN 318
           VSGSGVVSTP+     Q            ++FY L L+++SVG K+  +  S       N
Sbjct: 270 VSGSGVVSTPLIAKASQ------------ETFYYLTLKSISVGSKQIQYSGSDSESSEGN 329

Query: 319 IVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPITMHF 378
           I+IDSGTTLT LP + Y++   A++++I+ ++  DP   L  C+ + T D K P ITMHF
Sbjct: 330 IIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCY-SATGDLKVPVITMHF 389

Query: 379 EGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSFKPT 438
           +GADV L   N F++VS D+VC AF         +YGN+AQ NFL+GYD  +  VSFKPT
Sbjct: 390 DGADVKLDSSNAFVQVSEDLVCFAFRGSP--SFSIYGNVAQMNFLVGYDTVSKTVSFKPT 437

Query: 439 NCVAM 441
           +C  M
Sbjct: 450 DCAKM 437

[Close]

BLAST of Lsi09G018790.1 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 3.0e-86
Identity = 181/436 (41.51%), Postives = 260/436 (59.63%), Query Frame = 0


Query: 20  FTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRN---TVALTTVTAEAPIYNNRGEY 79
           F++ELIHRDSP SP+YNP  T  DRL  A  RS++R+      L+    ++ +    GE+
Sbjct: 26  FSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRFNHQLSQTDLQSGLIGADGEF 85

Query: 80  LMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSSIC 139
            M I++GTPP  + A+ADTGSD+ W QC+PCQ C+++N P+F+  KS TYK   C S  C
Sbjct: 86  FMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENGPIFDKKKSSTYKSEPCDSRNC 145

Query: 140 LFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGCGH 199
             A  S+        + C Y  +YGD+S +KGD A +TV++ S SG PV+FP T  GCG+
Sbjct: 146 Q-ALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSIDSASGSPVSFPGTVFGCGY 205

Query: 200 DNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSNADV 259
           +N GTF    SGI+GLG G  SL+ Q+G +   KFSYCL+   +    ++ +N G+N+  
Sbjct: 206 NNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCLSHKSATTNGTSVINLGTNSIP 265

Query: 260 SG----SGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPVSS---- 319
           S     SGVVSTP  +  K+ +T           +Y L LEA+SVG+KK  +  SS    
Sbjct: 266 SSLSKDSGVVSTP--LVDKEPLT-----------YYYLTLEAISVGKKKIPYTGSSYNPN 325

Query: 320 ----VLGGGANIVIDSGTTLTFLPPDLYNDFSTAISNAI-NLQRTNDPDQVLDCCFATTT 379
               +     NI+IDSGTTLT L    ++ FS+A+  ++   +R +DP  +L  CF + +
Sbjct: 326 DDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGLLSHCFKSGS 385

Query: 380 DDYKTPPITMHFEGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGY 439
            +   P IT+HF GADV LS  N F+++S D+VCL+     E  + +YGN AQ +FL+GY
Sbjct: 386 AEIGLPEITVHFTGADVRLSPINAFVKLSEDMVCLSMVPTTE--VAIYGNFAQMDFLVGY 445

[Close]

BLAST of Lsi09G018790.1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 8.6e-65
Identity = 163/437 (37.30%), Postives = 220/437 (50.34%), Query Frame = 0


Query: 13  HQSRDYGFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINR----NTVALTTVTAEAP 72
           H+++  GF I L H DS K+       T F  L  A+ R   R      +       E  
Sbjct: 34  HEAKVTGFQIMLEHVDSGKN------LTKFQLLERAIERGSRRLQRLEAMLNGPSGVETS 93

Query: 73  IYNNRGEYLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKK 132
           +Y   GEYLM +S+GTP     A+ DTGSD+IWTQCQPC  CF Q+ P+FNP  S ++  
Sbjct: 94  VYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFST 153

Query: 133 VACSSSICLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFP 192
           + CSS +C  A  S +CS+    + C Y+  YGD S T+G    +T+T GS     V+ P
Sbjct: 154 LPCSSQLCQ-ALSSPTCSN----NFCQYTYGYGDGSETQGSMGTETLTFGS-----VSIP 213

Query: 193 RTAIGCGHDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKL 252
               GCG +N G    N +G+VG+G GP SL  Q+      KFSYC+TPIGS     + L
Sbjct: 214 NITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVT---KFSYCMTPIGSST--PSNL 273

Query: 253 NFGSNADVSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPVSS 312
             GS A+   +G  +T + I   QI T           FY + L  +SVG  +     S+
Sbjct: 274 LLGSLANSVTAGSPNTTL-IQSSQIPT-----------FYYITLNGLSVGSTRLPIDPSA 333

Query: 313 VL----GGGANIVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTD 372
                  G   I+IDSGTTLT+   + Y        + INL   N      D CF T +D
Sbjct: 334 FALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSD 393

Query: 373 --DYKTPPITMHFEGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIG 432
             + + P   MHF+G D+ L  EN FI  SN ++CLA  +  + G+ ++GNI Q N L+ 
Sbjct: 394 PSNLQIPTFVMHFDGGDLELPSENYFISPSNGLICLAMGSSSQ-GMSIFGNIQQQNMLVV 436

Query: 433 YDIKNMFVSFKPTNCVA 440
           YD  N  VSF    C A
Sbjct: 454 YDTGNSVVSFASAQCGA 436

[Close]

BLAST of Lsi09G018790.1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 5.6e-64
Identity = 153/430 (35.58%), Postives = 228/430 (53.02%), Query Frame = 0


Query: 19  GFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINR----NTVALTTVTAEAPIYNNRG 78
           G  ++L   DS K+       T ++ +  A++R   R    N +  ++   E P+Y   G
Sbjct: 41  GLRVDLEQVDSGKN------LTKYELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDG 100

Query: 79  EYLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSS 138
           EYLM +++GTP  S  A+ DTGSD+IWTQC+PC  CF Q  P+FNP  S ++  + C S 
Sbjct: 101 EYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQ 160

Query: 139 ICLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGC 198
            C     S +C++    ++C Y+  YGD S T+G  A +T T  ++S      P  A GC
Sbjct: 161 YCQDL-PSETCNN----NECQYTYGYGDGSTTQGYMATETFTFETSS-----VPNIAFGC 220

Query: 199 GHDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSNA 258
           G DN G    N +G++G+G GP SL  Q+G    G+FSYC+T  GS +   + L  GS A
Sbjct: 221 GEDNQGFGQGNGAGLIGMGWGPLSLPSQLGV---GQFSYCMTSYGSSS--PSTLALGSAA 280

Query: 259 DVSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPVSSVL---G 318
                G  ST +  S          SLN   ++Y + L+ ++VG      P S+      
Sbjct: 281 SGVPEGSPSTTLIHS----------SLN--PTYYYITLQGITVGGDNLGIPSSTFQLQDD 340

Query: 319 GGANIVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTD--DYKTP 378
           G   ++IDSGTTLT+LP D YN  + A ++ INL   ++    L  CF   +D    + P
Sbjct: 341 GTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVP 400

Query: 379 PITMHFEGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMF 438
            I+M F+G  + L ++N+ I  +  V+CLA  +  + GI ++GNI Q    + YD++N+ 
Sbjct: 401 EISMQFDGGVLNLGEQNILISPAEGVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLA 437

Query: 439 VSFKPTNCVA 440
           VSF PT C A
Sbjct: 461 VSFVPTQCGA 437

[Close]

BLAST of Lsi09G018790.1 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 3.3e-48
Identity = 135/371 (36.39%), Postives = 189/371 (50.94%), Query Frame = 0


Query: 74  GEYLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSS 133
           GEY  ++ +GTP   +  V DTGSDI+W QC PC+ C+ Q+ P+F+P KS TY  + CSS
Sbjct: 140 GEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSS 199

Query: 134 SICLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIG 193
             C    DS+ C+++  +  CLY ++YGD S T GDF+ +T+T      + V     A+G
Sbjct: 200 PHCRRL-DSAGCNTR--RKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGV-----ALG 259

Query: 194 CGHDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSN 253
           CGHDN G F    +G++GLG G  S   Q G     KFSYCL    S + + + + FG N
Sbjct: 260 CGHDNEGLF-VGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVD-RSASSKPSSVVFG-N 319

Query: 254 ADVSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPVSSVLG-- 313
           A VS             +    T L S     +FY + L  +SVG  +     +S+    
Sbjct: 320 AAVS-------------RIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLD 379

Query: 314 --GGANIVIDSGTTLTFLPPDLYNDFSTAIS-NAINLQRTNDPDQVLDCCF-ATTTDDYK 373
             G   ++IDSGT++T L    Y     A    A  L+R  D   + D CF  +  ++ K
Sbjct: 380 QIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPD-FSLFDTCFDLSNMNEVK 439

Query: 374 TPPITMHFEGADVPLSQENVFIRV-SNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIK 433
            P + +HF GADV L   N  I V +N   C AF AG   G+ + GNI Q  F + YD+ 
Sbjct: 440 VPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAF-AGTMGGLSIIGNIQQQGFRVVYDLA 484

Query: 434 NMFVSFKPTNC 438
           +  V F P  C
Sbjct: 500 SSRVGFAPGGC 484

[Close]

BLAST of Lsi09G018790.1 vs. ExPASy TrEMBL
Match: A0A1S4E2N4 (aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC107991689 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 2.3e-169
Identity = 296/428 (69.16%), Postives = 352/428 (82.24%), Query Frame = 0


Query: 15  SRDYGFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYNNRG 74
           +RDYGFT+ELIHRDS KSPMYN  +TH+DR+ +ALRRSINRN   LT+ TAEAPIYNN G
Sbjct: 21  ARDYGFTVELIHRDSTKSPMYNSSETHYDRIANALRRSINRNKAVLTSDTAEAPIYNNGG 80

Query: 75  EYLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSS 134
           EYL++IS+GTPPFSI+AVADTGSD+IWTQC+PC NC+QQ+APMF+PSKS TYK V CSS 
Sbjct: 81  EYLVEISIGTPPFSILAVADTGSDVIWTQCEPCSNCYQQSAPMFDPSKSATYKNVPCSSP 140

Query: 135 ICLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGC 194
           +C ++GD SSCS     S+CLYSI YGD+SH+ G+ AVDTVTM STSGRPVAFPRT IGC
Sbjct: 141 VCSYSGDGSSCSD---DSECLYSIAYGDKSHSDGNLAVDTVTMQSTSGRPVAFPRTVIGC 200

Query: 195 GHDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIE-SNKLNFGSN 254
           GHDNAGTF+AN+SGIVGLG GPASLV Q+GPA GGKFSYCL PIG+ ++E S KLNFGSN
Sbjct: 201 GHDNAGTFNANVSGIVGLGRGPASLVTQLGPATGGKFSYCLMPIGNASMEDSTKLNFGSN 260

Query: 255 ADVSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFP-VSSVLGG 314
           ADVSGSG VSTPI+ S            + +K+FYSL LEAVSVG+ KFDFP VSS LGG
Sbjct: 261 ADVSGSGAVSTPIYTS------------DQYKTFYSLKLEAVSVGDNKFDFPEVSSKLGG 320

Query: 315 GANIVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPIT 374
            ANI+IDSGTTLT+LP DL ++F +AI+++INL R  DP Q LD CF+TTTDDY+ P +T
Sbjct: 321 EANIIIDSGTTLTYLPSDLMSNFGSAIADSINLPRAEDPSQFLDYCFSTTTDDYEVPSVT 380

Query: 375 MHFEGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSF 434
           MHFEGADVPL +EN+FIR+S D +CLAF A  ++ I +YGNIAQ NFL+GYDIKN+ VSF
Sbjct: 381 MHFEGADVPLQRENMFIRLSEDTICLAFGAFSDDNIFIYGNIAQSNFLVGYDIKNLAVSF 433

Query: 435 KPTNCVAM 441
           +P +C AM
Sbjct: 441 QPADCNAM 433

[Close]

BLAST of Lsi09G018790.1 vs. ExPASy TrEMBL
Match: A0A0A0K9V4 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G078630 PE=3 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 3.0e-169
Identity = 300/425 (70.59%), Postives = 350/425 (82.35%), Query Frame = 0


Query: 17  DYGFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYNNRGEY 76
           DYGFT+ELIHRDSPKSPMYNPL+ H+ R+ D LRRSI+ NT  L T T EAPIYNNRGEY
Sbjct: 27  DYGFTVELIHRDSPKSPMYNPLENHYHRVADTLRRSISHNT-GLVTNTVEAPIYNNRGEY 86

Query: 77  LMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSSIC 136
           LMK+S+GTPPF IIAVADTGSDIIWTQC+PC NC+QQ+ PMFNPSKS TY+KV+CSS +C
Sbjct: 87  LMKLSVGTPPFPIIAVADTGSDIIWTQCEPCTNCYQQDLPMFNPSKSTTYRKVSCSSPVC 146

Query: 137 LFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGCGH 196
            F G+ +SC   SF+ DC YSI+YGD SH++GDFAVDT+TMGSTSGR VAFPRTAIGCGH
Sbjct: 147 SFTGEDNSC---SFKPDCTYSISYGDNSHSQGDFAVDTLTMGSTSGRVVAFPRTAIGCGH 206

Query: 197 DNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSNADV 256
           DNAG+F AN+SGIVGLGLGPASL+KQMG A GGKFSYCLTPIG+ +  SNKLNFGSNA+V
Sbjct: 207 DNAGSFDANVSGIVGLGLGPASLIKQMGSAVGGKFSYCLTPIGNDDGGSNKLNFGSNANV 266

Query: 257 SGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKK-FDFPVSSVLGGGAN 316
           SGSG VSTPI+IS K            FKSFYSL L+AVSVG    F    +S+LGG AN
Sbjct: 267 SGSGAVSTPIYISDK------------FKSFYSLKLKAVSVGRNNTFYSTANSILGGKAN 326

Query: 317 IVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPITMHF 376
           I+IDSGTTLT LP DLY++F+ AISN+INLQRT+DP+Q L+ CF TTTDDYK P I MHF
Sbjct: 327 IIIDSGTTLTLLPVDLYHNFAKAISNSINLQRTDDPNQFLEYCFETTTDDYKVPFIAMHF 386

Query: 377 EGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSFKPT 436
           EGA++ L +ENV IRVS++V+CLAF+  Q+N I +YGNIAQ NFL+GYD+ NM +SFKP 
Sbjct: 387 EGANLRLQRENVLIRVSDNVICLAFAGAQDNDISIYGNIAQINFLVGYDVTNMSLSFKPM 435

Query: 437 NCVAM 441
           NCVAM
Sbjct: 447 NCVAM 435

[Close]

BLAST of Lsi09G018790.1 vs. ExPASy TrEMBL
Match: A0A5D3DLM9 (Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00670 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 6.6e-169
Identity = 295/428 (68.93%), Postives = 351/428 (82.01%), Query Frame = 0


Query: 15  SRDYGFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYNNRG 74
           +RDYGFT+ELIHRDS KSPMYN  +TH+DR+ +ALRRSINRN   LT+ TAEAPIYNN G
Sbjct: 21  ARDYGFTVELIHRDSTKSPMYNSSETHYDRIANALRRSINRNKAVLTSDTAEAPIYNNGG 80

Query: 75  EYLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSS 134
           EYL++IS+GTPPFSI+AVADTGSD+IWTQC+PC NC+QQ+APMF+PSKS TYK V CSS 
Sbjct: 81  EYLVEISIGTPPFSILAVADTGSDVIWTQCEPCSNCYQQSAPMFDPSKSATYKNVPCSSP 140

Query: 135 ICLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGC 194
           +C ++GD SSCS     S+CLYSI YGD+SH+ G+ AVDTVTM STSGRPV+FPRT IGC
Sbjct: 141 VCSYSGDGSSCSD---DSECLYSIAYGDKSHSDGNLAVDTVTMQSTSGRPVSFPRTVIGC 200

Query: 195 GHDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIE-SNKLNFGSN 254
           GHDNAGTF+AN+SGIVGLG GPASLV Q+GPA GGKFSYCL PIG+ ++E S KLNFGSN
Sbjct: 201 GHDNAGTFNANVSGIVGLGRGPASLVTQLGPATGGKFSYCLMPIGNASMEDSTKLNFGSN 260

Query: 255 ADVSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFP-VSSVLGG 314
           ADVSGSG VSTPI+ S            + +K+FYSL LEAVSVG+ KFDFP VSS LGG
Sbjct: 261 ADVSGSGAVSTPIYTS------------DQYKTFYSLKLEAVSVGDNKFDFPEVSSKLGG 320

Query: 315 GANIVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPIT 374
            ANI+IDSGTTLT+LP DL ++F +AI+++INL R  DP Q LD CF+TTTDDY+ P +T
Sbjct: 321 EANIIIDSGTTLTYLPSDLMSNFGSAIADSINLPRAEDPSQFLDYCFSTTTDDYEVPSVT 380

Query: 375 MHFEGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSF 434
           MHFEGADVPL +EN+FIR+S D +CLAF A  ++ I +YGNIAQ NFL+GYDIKN+ VSF
Sbjct: 381 MHFEGADVPLQRENMFIRLSEDTICLAFGAFSDDNIFIYGNIAQSNFLVGYDIKNLAVSF 433

Query: 435 KPTNCVAM 441
           +P  C AM
Sbjct: 441 QPAECNAM 433

[Close]

BLAST of Lsi09G018790.1 vs. ExPASy TrEMBL
Match: A0A0A0K928 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G078650 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 5.8e-165
Identity = 288/428 (67.29%), Postives = 350/428 (81.78%), Query Frame = 0


Query: 15  SRDYGFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYNNRG 74
           +RDYGFT+ELIHRDSPKSPMYN  +THFDR+ +ALRRS +RNTV L + TAEAPI+NN G
Sbjct: 22  ARDYGFTVELIHRDSPKSPMYNSSETHFDRIVNALRRSSHRNTVVLESDTAEAPIFNNGG 81

Query: 75  EYLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSS 134
           EYL++IS+GTPPFSI+AVADTGSD+IWTQC+PC NC+QQNAPMF+PSKS TYK VACSS 
Sbjct: 82  EYLVEISVGTPPFSIVAVADTGSDVIWTQCKPCSNCYQQNAPMFDPSKSTTYKNVACSSP 141

Query: 135 ICLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGC 194
           +C ++GD SSCS     S+CLYSI YGD SH++G+ AVDTVTM STSGRPVAFPRT IGC
Sbjct: 142 VCSYSGDGSSCSD---DSECLYSIAYGDDSHSQGNLAVDTVTMQSTSGRPVAFPRTVIGC 201

Query: 195 GHDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNI-ESNKLNFGSN 254
           GHDNAGTF+AN+SGIVGLG GPASLV Q+GPA GGKFSYCL PIG+ +  +S KLNFGSN
Sbjct: 202 GHDNAGTFNANVSGIVGLGRGPASLVTQLGPATGGKFSYCLIPIGTGSTNDSTKLNFGSN 261

Query: 255 ADVSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPV-SSVLGG 314
           A+VSGSG VSTPI+ S +            +K+FYSL LEAVSVG+ KF+FP  +S LGG
Sbjct: 262 ANVSGSGTVSTPIYSSAQ------------YKTFYSLKLEAVSVGDTKFNFPEGASKLGG 321

Query: 315 GANIVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPIT 374
            +NI+IDSGTTLT+LP  L N F +AIS +++L    DP + LD CFATTTDDY+ PP+T
Sbjct: 322 ESNIIIDSGTTLTYLPSALLNSFGSAISQSMSLPHAQDPSEFLDYCFATTTDDYEMPPVT 381

Query: 375 MHFEGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSF 434
           MHFEGADVPL +EN+F+R+S+D +CLAF +  ++ I +YGNIAQ NFL+GYDIKN+ VSF
Sbjct: 382 MHFEGADVPLQRENLFVRLSDDTICLAFGSFPDDNIFIYGNIAQSNFLVGYDIKNLAVSF 434

Query: 435 KPTNCVAM 441
           +P +C A+
Sbjct: 442 QPAHCGAV 434

[Close]

BLAST of Lsi09G018790.1 vs. ExPASy TrEMBL
Match: A0A5A7TAJ2 (Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00660 PE=3 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.7e-161
Identity = 293/424 (69.10%), Postives = 344/424 (81.13%), Query Frame = 0


Query: 19  GFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYNNRGEYLM 78
           GFT+ELIHRDS KSPMYNP + H+ R+ + LRRSI+RNT  + T T EAPI+NNRGEYLM
Sbjct: 27  GFTVELIHRDSRKSPMYNPSENHYLRVANTLRRSISRNTAGVVTNTVEAPIFNNRGEYLM 86

Query: 79  KISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSSICLF 138
           K+SLGTPPF IIAVADTGSDIIWTQC+PC +C++Q+APMFNPSKS TY KV+CSS IC F
Sbjct: 87  KLSLGTPPFPIIAVADTGSDIIWTQCEPCIDCYKQDAPMFNPSKSTTYSKVSCSSPICSF 146

Query: 139 AGDS-SSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGCGHD 198
            GD   SCSS    S+C+YSI+YGD SH++GDFA+DT++M STSGR VAFPRTAIGCGHD
Sbjct: 147 TGDDRRSCSS---TSECMYSISYGDNSHSEGDFALDTLSMDSTSGRLVAFPRTAIGCGHD 206

Query: 199 NAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSNADVS 258
           N+GTF AN+SGIVGLGLGPASLVKQMG A  GKFSYCLTPIGS +++SNKLNFGSNA+VS
Sbjct: 207 NSGTFDANVSGIVGLGLGPASLVKQMGSAVAGKFSYCLTPIGSDDVKSNKLNFGSNANVS 266

Query: 259 GSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKK-FDFPVSSVLGGGANI 318
           GSG VSTPI+IS K            FKSFYSL L+AVSV  K  F     S + G ANI
Sbjct: 267 GSGAVSTPIYISDK------------FKSFYSLKLKAVSVERKNIFYVRARSSILGEANI 326

Query: 319 VIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPITMHFE 378
           +IDSGTTLT LP D+Y +F+ AISN+INLQRT+DP++ L+ CFATTTDDYK P I MHFE
Sbjct: 327 IIDSGTTLTLLPADVYQNFAEAISNSINLQRTDDPNRFLNYCFATTTDDYKMPHIAMHFE 386

Query: 379 GADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSFKPTN 438
           GA+V L +ENV +RVS+DVVCLAF++ Q+N I +YGNIAQ NFL+GYDI NM +SFK  N
Sbjct: 387 GANVRLHRENVLVRVSDDVVCLAFASSQDNDISIYGNIAQINFLVGYDINNMSISFKRAN 435

Query: 439 CVAM 441
           CVAM
Sbjct: 447 CVAM 435

[Close]

BLAST of Lsi09G018790.1 vs. NCBI nr
Match: XP_038907012.1 (aspartic proteinase CDR1-like [Benincasa hispida])

HSP 1 Score: 661.4 bits (1705), Expect = 5.5e-186
Identity = 329/425 (77.41%), Postives = 366/425 (86.12%), Query Frame = 0


Query: 16  RDYGFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYNNRGE 75
           RDYGF++ELIHRDSPKSPMYNP KTH+ RL D+LRRSI+RNT ALT + AEAPIYNN+GE
Sbjct: 22  RDYGFSVELIHRDSPKSPMYNPSKTHYHRLADSLRRSISRNTAALTDI-AEAPIYNNQGE 81

Query: 76  YLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSSI 135
           YLMKIS+GTPPFSIIAVADTGSDI WTQC PC+NCF QNAPMFNPSKS TYKKVACSS I
Sbjct: 82  YLMKISVGTPPFSIIAVADTGSDITWTQCHPCKNCFHQNAPMFNPSKSTTYKKVACSSPI 141

Query: 136 CLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGCG 195
           CLFAGDS SCS+QS +SDC+YSITYGDRSH+KGDFAVDTVTMGSTSGR VAFPR AIGCG
Sbjct: 142 CLFAGDSGSCSTQSLESDCVYSITYGDRSHSKGDFAVDTVTMGSTSGRGVAFPRMAIGCG 201

Query: 196 HDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSNAD 255
           HDNAGTF+ NISGIVGLGLGPASLV QMGPA GGKFSYCLTP+GS NI  +KLNFGSNAD
Sbjct: 202 HDNAGTFNVNISGIVGLGLGPASLVTQMGPATGGKFSYCLTPVGSSNIVPSKLNFGSNAD 261

Query: 256 VSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPVSSVLGGGAN 315
           VSGS  VSTPI+ S K            FK FYS+ LEAVSVG+ KFDFPV SVLGG AN
Sbjct: 262 VSGSKAVSTPIYTSDK------------FK-FYSVKLEAVSVGKNKFDFPVYSVLGGKAN 321

Query: 316 IVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPITMHF 375
           I++DSG+TLTFLP +LYN+FS AIS ++NL+RTNDP+Q+LD CF TTTDDYK P ITMHF
Sbjct: 322 IILDSGSTLTFLPVNLYNNFSKAISKSLNLKRTNDPEQLLDHCFVTTTDDYKLPLITMHF 381

Query: 376 EGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSFKPT 435
           EGADVPL +ENVFIRVS+ VVCLAF   Q++  M++GNIAQ NFL+GYDIKNM VSFKP 
Sbjct: 382 EGADVPLLRENVFIRVSDSVVCLAFGTHQDDDFMIFGNIAQTNFLVGYDIKNMLVSFKPM 432

Query: 436 NCVAM 441
           +CVAM
Sbjct: 442 DCVAM 432

[Close]

BLAST of Lsi09G018790.1 vs. NCBI nr
Match: XP_038876324.1 (aspartic proteinase CDR1-like [Benincasa hispida])

HSP 1 Score: 620.9 bits (1600), Expect = 8.3e-174
Identity = 310/428 (72.43%), Postives = 359/428 (83.88%), Query Frame = 0


Query: 16  RDYGFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYNNRGE 75
           R++GFT+ELIHRDSPKSPMYNP +TH+ RL +ALRRSI+RNT A+T  TA APIYN RG+
Sbjct: 24  REFGFTVELIHRDSPKSPMYNPSETHYHRLANALRRSISRNTAAVTD-TAVAPIYNYRGQ 83

Query: 76  YLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSSI 135
           YLMKISLGTPPFSIIAVADTGSD+IWTQC+PC NC++Q+APMFNPSKS TYK V CSS I
Sbjct: 84  YLMKISLGTPPFSIIAVADTGSDVIWTQCEPCPNCYEQSAPMFNPSKSTTYKNVPCSSPI 143

Query: 136 CLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGCG 195
           C +AG+ SSCS+    S+CLYSI+YGDRSH++GDFAVDTVTMGSTSG PV FP  AIGCG
Sbjct: 144 CSYAGEDSSCSA---HSECLYSISYGDRSHSQGDFAVDTVTMGSTSGSPVTFPHMAIGCG 203

Query: 196 HDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSNAD 255
           HDNAGTF A++SGIVGLG G ASLV QMGPA GGKFSYCL PIG+ + ES+KLNFGSNAD
Sbjct: 204 HDNAGTFDASVSGIVGLGQGSASLVSQMGPATGGKFSYCLAPIGNSSAESSKLNFGSNAD 263

Query: 256 VSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFP-VSSVLGGGA 315
           VSGS  VSTPI+ S K            +K+FYSL LEAVSVGE KFDFP VSS LGG  
Sbjct: 264 VSGSEAVSTPIYTSVK------------YKTFYSLKLEAVSVGENKFDFPIVSSRLGGEG 323

Query: 316 NIVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPITMH 375
           NI+IDSGTTLTFLP DLYN+F+T IS++INLQRT+DP+Q LD CFATTTDDY+ P +TMH
Sbjct: 324 NIIIDSGTTLTFLPVDLYNNFATTISDSINLQRTDDPNQFLDYCFATTTDDYEAPSVTMH 383

Query: 376 FEGADVPLSQENVFIRVSNDVVCLAFSAGQENG--IMLYGNIAQGNFLIGYDIKNMFVSF 435
           FEGADVPL++ENVFIR+S+D+VCLAF A Q++   I +YGNI+Q NFL+GYDIKNM VSF
Sbjct: 384 FEGADVPLNRENVFIRISDDIVCLAFKASQDDQEMIFIYGNISQNNFLVGYDIKNMVVSF 435

Query: 436 KPTNCVAM 441
           K  +CVAM
Sbjct: 444 KQADCVAM 435

[Close]

BLAST of Lsi09G018790.1 vs. NCBI nr
Match: XP_016902483.1 (PREDICTED: aspartic proteinase CDR1-like [Cucumis melo])

HSP 1 Score: 605.1 bits (1559), Expect = 4.7e-169
Identity = 296/428 (69.16%), Postives = 352/428 (82.24%), Query Frame = 0


Query: 15  SRDYGFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYNNRG 74
           +RDYGFT+ELIHRDS KSPMYN  +TH+DR+ +ALRRSINRN   LT+ TAEAPIYNN G
Sbjct: 21  ARDYGFTVELIHRDSTKSPMYNSSETHYDRIANALRRSINRNKAVLTSDTAEAPIYNNGG 80

Query: 75  EYLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSS 134
           EYL++IS+GTPPFSI+AVADTGSD+IWTQC+PC NC+QQ+APMF+PSKS TYK V CSS 
Sbjct: 81  EYLVEISIGTPPFSILAVADTGSDVIWTQCEPCSNCYQQSAPMFDPSKSATYKNVPCSSP 140

Query: 135 ICLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGC 194
           +C ++GD SSCS     S+CLYSI YGD+SH+ G+ AVDTVTM STSGRPVAFPRT IGC
Sbjct: 141 VCSYSGDGSSCSD---DSECLYSIAYGDKSHSDGNLAVDTVTMQSTSGRPVAFPRTVIGC 200

Query: 195 GHDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIE-SNKLNFGSN 254
           GHDNAGTF+AN+SGIVGLG GPASLV Q+GPA GGKFSYCL PIG+ ++E S KLNFGSN
Sbjct: 201 GHDNAGTFNANVSGIVGLGRGPASLVTQLGPATGGKFSYCLMPIGNASMEDSTKLNFGSN 260

Query: 255 ADVSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFP-VSSVLGG 314
           ADVSGSG VSTPI+ S            + +K+FYSL LEAVSVG+ KFDFP VSS LGG
Sbjct: 261 ADVSGSGAVSTPIYTS------------DQYKTFYSLKLEAVSVGDNKFDFPEVSSKLGG 320

Query: 315 GANIVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPIT 374
            ANI+IDSGTTLT+LP DL ++F +AI+++INL R  DP Q LD CF+TTTDDY+ P +T
Sbjct: 321 EANIIIDSGTTLTYLPSDLMSNFGSAIADSINLPRAEDPSQFLDYCFSTTTDDYEVPSVT 380

Query: 375 MHFEGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSF 434
           MHFEGADVPL +EN+FIR+S D +CLAF A  ++ I +YGNIAQ NFL+GYDIKN+ VSF
Sbjct: 381 MHFEGADVPLQRENMFIRLSEDTICLAFGAFSDDNIFIYGNIAQSNFLVGYDIKNLAVSF 433

Query: 435 KPTNCVAM 441
           +P +C AM
Sbjct: 441 QPADCNAM 433

[Close]

BLAST of Lsi09G018790.1 vs. NCBI nr
Match: XP_031743830.1 (aspartic proteinase CDR1-like [Cucumis sativus] >KGN46268.1 hypothetical protein Csa_005375 [Cucumis sativus])

HSP 1 Score: 604.7 bits (1558), Expect = 6.1e-169
Identity = 300/425 (70.59%), Postives = 350/425 (82.35%), Query Frame = 0


Query: 17  DYGFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYNNRGEY 76
           DYGFT+ELIHRDSPKSPMYNPL+ H+ R+ D LRRSI+ NT  L T T EAPIYNNRGEY
Sbjct: 27  DYGFTVELIHRDSPKSPMYNPLENHYHRVADTLRRSISHNT-GLVTNTVEAPIYNNRGEY 86

Query: 77  LMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSSIC 136
           LMK+S+GTPPF IIAVADTGSDIIWTQC+PC NC+QQ+ PMFNPSKS TY+KV+CSS +C
Sbjct: 87  LMKLSVGTPPFPIIAVADTGSDIIWTQCEPCTNCYQQDLPMFNPSKSTTYRKVSCSSPVC 146

Query: 137 LFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGCGH 196
            F G+ +SC   SF+ DC YSI+YGD SH++GDFAVDT+TMGSTSGR VAFPRTAIGCGH
Sbjct: 147 SFTGEDNSC---SFKPDCTYSISYGDNSHSQGDFAVDTLTMGSTSGRVVAFPRTAIGCGH 206

Query: 197 DNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSNADV 256
           DNAG+F AN+SGIVGLGLGPASL+KQMG A GGKFSYCLTPIG+ +  SNKLNFGSNA+V
Sbjct: 207 DNAGSFDANVSGIVGLGLGPASLIKQMGSAVGGKFSYCLTPIGNDDGGSNKLNFGSNANV 266

Query: 257 SGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKK-FDFPVSSVLGGGAN 316
           SGSG VSTPI+IS K            FKSFYSL L+AVSVG    F    +S+LGG AN
Sbjct: 267 SGSGAVSTPIYISDK------------FKSFYSLKLKAVSVGRNNTFYSTANSILGGKAN 326

Query: 317 IVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPITMHF 376
           I+IDSGTTLT LP DLY++F+ AISN+INLQRT+DP+Q L+ CF TTTDDYK P I MHF
Sbjct: 327 IIIDSGTTLTLLPVDLYHNFAKAISNSINLQRTDDPNQFLEYCFETTTDDYKVPFIAMHF 386

Query: 377 EGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSFKPT 436
           EGA++ L +ENV IRVS++V+CLAF+  Q+N I +YGNIAQ NFL+GYD+ NM +SFKP 
Sbjct: 387 EGANLRLQRENVLIRVSDNVICLAFAGAQDNDISIYGNIAQINFLVGYDVTNMSLSFKPM 435

Query: 437 NCVAM 441
           NCVAM
Sbjct: 447 NCVAM 435

[Close]

BLAST of Lsi09G018790.1 vs. NCBI nr
Match: KAA0039977.1 (aspartic proteinase CDR1-like [Cucumis melo var. makuwa] >TYK24525.1 aspartic proteinase CDR1-like [Cucumis melo var. makuwa])

HSP 1 Score: 603.6 bits (1555), Expect = 1.4e-168
Identity = 295/428 (68.93%), Postives = 351/428 (82.01%), Query Frame = 0


Query: 15  SRDYGFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYNNRG 74
           +RDYGFT+ELIHRDS KSPMYN  +TH+DR+ +ALRRSINRN   LT+ TAEAPIYNN G
Sbjct: 21  ARDYGFTVELIHRDSTKSPMYNSSETHYDRIANALRRSINRNKAVLTSDTAEAPIYNNGG 80

Query: 75  EYLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSS 134
           EYL++IS+GTPPFSI+AVADTGSD+IWTQC+PC NC+QQ+APMF+PSKS TYK V CSS 
Sbjct: 81  EYLVEISIGTPPFSILAVADTGSDVIWTQCEPCSNCYQQSAPMFDPSKSATYKNVPCSSP 140

Query: 135 ICLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGC 194
           +C ++GD SSCS     S+CLYSI YGD+SH+ G+ AVDTVTM STSGRPV+FPRT IGC
Sbjct: 141 VCSYSGDGSSCSD---DSECLYSIAYGDKSHSDGNLAVDTVTMQSTSGRPVSFPRTVIGC 200

Query: 195 GHDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIE-SNKLNFGSN 254
           GHDNAGTF+AN+SGIVGLG GPASLV Q+GPA GGKFSYCL PIG+ ++E S KLNFGSN
Sbjct: 201 GHDNAGTFNANVSGIVGLGRGPASLVTQLGPATGGKFSYCLMPIGNASMEDSTKLNFGSN 260

Query: 255 ADVSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFP-VSSVLGG 314
           ADVSGSG VSTPI+ S            + +K+FYSL LEAVSVG+ KFDFP VSS LGG
Sbjct: 261 ADVSGSGAVSTPIYTS------------DQYKTFYSLKLEAVSVGDNKFDFPEVSSKLGG 320

Query: 315 GANIVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPIT 374
            ANI+IDSGTTLT+LP DL ++F +AI+++INL R  DP Q LD CF+TTTDDY+ P +T
Sbjct: 321 EANIIIDSGTTLTYLPSDLMSNFGSAIADSINLPRAEDPSQFLDYCFSTTTDDYEVPSVT 380

Query: 375 MHFEGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSF 434
           MHFEGADVPL +EN+FIR+S D +CLAF A  ++ I +YGNIAQ NFL+GYDIKN+ VSF
Sbjct: 381 MHFEGADVPLQRENMFIRLSEDTICLAFGAFSDDNIFIYGNIAQSNFLVGYDIKNLAVSF 433

Query: 435 KPTNCVAM 441
           +P  C AM
Sbjct: 441 QPAECNAM 433

[Close]

BLAST of Lsi09G018790.1 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 414.8 bits (1065), Expect = 8.4e-116
Identity = 209/425 (49.18%), Postives = 283/425 (66.59%), Query Frame = 0


Query: 19  GFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINR---NTVALTTVTAEAPIYNNRGE 78
           GFT +LIHRDSPKSP YNP++T   RL +A+ RS+NR    T    T   +  + +N GE
Sbjct: 30  GFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRVFHFTEKDNTPQPQIDLTSNSGE 89

Query: 79  YLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSSI 138
           YLM +S+GTPPF I+A+ADTGSD++WTQC PC +C+ Q  P+F+P  S TYK V+CSSS 
Sbjct: 90  YLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCSSSQ 149

Query: 139 CLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGCG 198
           C    + +SCS+    + C YS++YGD S+TKG+ AVDT+T+GS+  RP+      IGCG
Sbjct: 150 CTALENQASCSTN--DNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGCG 209

Query: 199 HDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSNAD 258
           H+NAGTF+   SGIVGLG GP SL+KQ+G +  GKFSYCL P+ S+  +++K+NFG+NA 
Sbjct: 210 HNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYCLVPLTSKKDQTSKINFGTNAI 269

Query: 259 VSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPVSSVLGGGAN 318
           VSGSGVVSTP+     Q            ++FY L L+++SVG K+  +  S       N
Sbjct: 270 VSGSGVVSTPLIAKASQ------------ETFYYLTLKSISVGSKQIQYSGSDSESSEGN 329

Query: 319 IVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPITMHF 378
           I+IDSGTTLT LP + Y++   A++++I+ ++  DP   L  C+ + T D K P ITMHF
Sbjct: 330 IIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCY-SATGDLKVPVITMHF 389

Query: 379 EGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSFKPT 438
           +GADV L   N F++VS D+VC AF         +YGN+AQ NFL+GYD  +  VSFKPT
Sbjct: 390 DGADVKLDSSNAFVQVSEDLVCFAFRGSP--SFSIYGNVAQMNFLVGYDTVSKTVSFKPT 437

Query: 439 NCVAM 441
           +C  M
Sbjct: 450 DCAKM 437

[Close]

BLAST of Lsi09G018790.1 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 397.1 bits (1019), Expect = 1.8e-110
Identity = 213/428 (49.77%), Postives = 285/428 (66.59%), Query Frame = 0


Query: 19  GFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTA-----EAPIYNNR 78
           GFTI+LIHRDSPKSP YN  +T   R+ +A+RRS  R+T+  +   A     ++ I +NR
Sbjct: 25  GFTIDLIHRDSPKSPFYNSAETSSQRMRNAIRRSA-RSTLQFSNDDASPNSPQSFITSNR 84

Query: 79  GEYLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSS 138
           GEYLM IS+GTPP  I+A+ADTGSD+IWTQC PC++C+QQ +P+F+P +S TY+KV+CSS
Sbjct: 85  GEYLMNISIGTPPVPILAIADTGSDLIWTQCNPCEDCYQQTSPLFDPKESSTYRKVSCSS 144

Query: 139 SICLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIG 198
           S C  A + +SCS+   ++ C Y+ITYGD S+TKGD AVDTVTMGS+  RPV+     IG
Sbjct: 145 SQCR-ALEDASCSTD--ENTCSYTITYGDNSYTKGDVAVDTVTMGSSGRRPVSLRNMIIG 204

Query: 199 CGHDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSN 258
           CGH+N GTF    SGI+GLG G  SLV Q+  +  GKFSYCL P  S    ++K+NFG+N
Sbjct: 205 CGHENTGTFDPAGSGIIGLGGGSTSLVSQLRKSINGKFSYCLVPFTSETGLTSKINFGTN 264

Query: 259 ADVSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPVSSVLG-G 318
             VSG GVVST +                   ++Y LNLEA+SVG KK  F  S++ G G
Sbjct: 265 GIVSGDGVVSTSMVKKDP-------------ATYYFLNLEAISVGSKKIQF-TSTIFGTG 324

Query: 319 GANIVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPIT 378
             NIVIDSGTTLT LP + Y +  + +++ I  +R  DPD +L  C+  ++  +K P IT
Sbjct: 325 EGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDGILSLCYRDSS-SFKVPDIT 384

Query: 379 MHFEGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMFVSF 438
           +HF+G DV L   N F+ VS DV C AF+A ++  + ++GN+AQ NFL+GYD  +  VSF
Sbjct: 385 VHFKGGDVKLGNLNTFVAVSEDVSCFAFAANEQ--LTIFGNLAQMNFLVGYDTVSGTVSF 431

Query: 439 KPTNCVAM 441
           K T+C  M
Sbjct: 445 KKTDCSQM 431

[Close]

BLAST of Lsi09G018790.1 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 330.5 bits (846), Expect = 2.1e-90
Identity = 182/429 (42.42%), Postives = 256/429 (59.67%), Query Frame = 0


Query: 21  TIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYNNRGEYLMKI 80
           T+ELIHRDSP SP+YNP  T  DRL  A  RSI+R+    T    ++ + +N GEY M I
Sbjct: 30  TVELIHRDSPHSPLYNPHHTVSDRLNAAFLRSISRSRRFTTKTDLQSGLISNGGEYFMSI 89

Query: 81  SLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSSICLFAG 140
           S+GTPP  + A+ADTGSD+ W QC+PCQ C++QN+P+F+  KS TYK  +C S  C    
Sbjct: 90  SIGTPPSKVFAIADTGSDLTWVQCKPCQQCYKQNSPLFDKKKSSTYKTESCDSKTCQALS 149

Query: 141 DSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGCGHDNAG 200
           +      +S +  C Y  +YGD S TKGD A +T+++ S+SG  V+FP T  GCG++N G
Sbjct: 150 EHEEGCDES-KDICKYRYSYGDNSFTKGDVATETISIDSSSGSSVSFPGTVFGCGYNNGG 209

Query: 201 TFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSNADVSGSG 260
           TF    SGI+GLG GP SLV Q+G + G KFSYCL+   +    ++ +N G+N+      
Sbjct: 210 TFEETGSGIIGLGGGPLSLVSQLGSSIGKKFSYCLSHTAATTNGTSVINLGTNS------ 269

Query: 261 VVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPVSSVLGGG------- 320
           + S P   S    +TT L   +  +++Y L LEAV+VG+ K  +      GGG       
Sbjct: 270 IPSNPSKDSA--TLTTPLIQKDP-ETYYFLTLEAVTVGKTKLPY-----TGGGYGLNGKS 329

Query: 321 ----ANIVIDSGTTLTFLPPDLYNDFSTAISNAI-NLQRTNDPDQVLDCCFATTTDDYKT 380
                NI+IDSGTTLT L    Y+DF TA+  ++   +R +DP  +L  CF +   +   
Sbjct: 330 SKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGAKRVSDPQGLLTHCFKSGDKEIGL 389

Query: 381 PPITMHFEGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNM 438
           P ITMHF  ADV LS  N F++++ D VCL+     E  + +YGN+ Q +FL+GYD++  
Sbjct: 390 PAITMHFTNADVKLSPINAFVKLNEDTVCLSMIPTTE--VAIYGNMVQMDFLVGYDLETK 441

[Close]

BLAST of Lsi09G018790.1 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 320.5 bits (820), Expect = 2.2e-87
Identity = 181/436 (41.51%), Postives = 260/436 (59.63%), Query Frame = 0


Query: 20  FTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRN---TVALTTVTAEAPIYNNRGEY 79
           F++ELIHRDSP SP+YNP  T  DRL  A  RS++R+      L+    ++ +    GE+
Sbjct: 26  FSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRRFNHQLSQTDLQSGLIGADGEF 85

Query: 80  LMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVACSSSIC 139
            M I++GTPP  + A+ADTGSD+ W QC+PCQ C+++N P+F+  KS TYK   C S  C
Sbjct: 86  FMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENGPIFDKKKSSTYKSEPCDSRNC 145

Query: 140 LFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTAIGCGH 199
             A  S+        + C Y  +YGD+S +KGD A +TV++ S SG PV+FP T  GCG+
Sbjct: 146 Q-ALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSIDSASGSPVSFPGTVFGCGY 205

Query: 200 DNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFGSNADV 259
           +N GTF    SGI+GLG G  SL+ Q+G +   KFSYCL+   +    ++ +N G+N+  
Sbjct: 206 NNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCLSHKSATTNGTSVINLGTNSIP 265

Query: 260 SG----SGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPVSS---- 319
           S     SGVVSTP  +  K+ +T           +Y L LEA+SVG+KK  +  SS    
Sbjct: 266 SSLSKDSGVVSTP--LVDKEPLT-----------YYYLTLEAISVGKKKIPYTGSSYNPN 325

Query: 320 ----VLGGGANIVIDSGTTLTFLPPDLYNDFSTAISNAI-NLQRTNDPDQVLDCCFATTT 379
               +     NI+IDSGTTLT L    ++ FS+A+  ++   +R +DP  +L  CF + +
Sbjct: 326 DDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGLLSHCFKSGS 385

Query: 380 DDYKTPPITMHFEGADVPLSQENVFIRVSNDVVCLAFSAGQENGIMLYGNIAQGNFLIGY 439
            +   P IT+HF GADV LS  N F+++S D+VCL+     E  + +YGN AQ +FL+GY
Sbjct: 386 AEIGLPEITVHFTGADVRLSPINAFVKLSEDMVCLSMVPTTE--VAIYGNFAQMDFLVGY 445

[Close]

BLAST of Lsi09G018790.1 vs. TAIR 10
Match: AT2G28010.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 268.9 bits (686), Expect = 7.4e-72
Identity = 168/431 (38.98%), Postives = 231/431 (53.60%), Query Frame = 0


Query: 15  SRDYGFTIELIHRDSPKSPMYNPLKTHFDRLTDALRRSINRNTVALTTVTAEAPIYN--- 74
           S  +GFT++LIHR S                        N ++    T +  +P  N   
Sbjct: 25  SPPHGFTMDLIHRRS------------------------NASSRVSNTQSGSSPYANTVF 84

Query: 75  NRGEYLMKISLGTPPFSIIAVADTGSDIIWTQCQPCQNCFQQNAPMFNPSKSVTYKKVAC 134
           +   YLMK+ +GTPPF I A+ DTGS+I WTQC PC +C++QNAP+F+PSKS T+K+  C
Sbjct: 85  DNSVYLMKLQVGTPPFEIQAIIDTGSEITWTQCLPCVHCYEQNAPIFDPSKSSTFKEKRC 144

Query: 135 SSSICLFAGDSSSCSSQSFQSDCLYSITYGDRSHTKGDFAVDTVTMGSTSGRPVAFPRTA 194
                    D  SC          Y + Y D ++T G  A +T+T+ STSG P   P T 
Sbjct: 145 ---------DGHSCP---------YEVDYFDHTYTMGTLATETITLHSTSGEPFVMPETI 204

Query: 195 IGCGHDNAGTFSANISGIVGLGLGPASLVKQMGPAAGGKFSYCLTPIGSRNIESNKLNFG 254
           IGCGH+N+  F  + SG+VGL  GP+SL+ QMG    G  SYC +  G     ++K+NFG
Sbjct: 205 IGCGHNNS-WFKPSFSGMVGLNWGPSSLITQMGGEYPGLMSYCFSGQG-----TSKINFG 264

Query: 255 SNADVSGSGVVSTPIHISGKQIITTKLKSLNTFKSFYSLNLEAVSVGEKKFDFPVSSVLG 314
           +NA V+G GVVST + ++     T K         FY LNL+AVSVG  + +   ++   
Sbjct: 265 ANAIVAGDGVVSTTMFMT-----TAK-------PGFYYLNLDAVSVGNTRIETMGTTFHA 324

Query: 315 GGANIVIDSGTTLTFLPPDLYNDFSTAISNAINLQRTNDPDQVLDCCFATTTDDYKTPPI 374
              NIVIDSGTTLT+ P    N    A+ + +   R  DP      C+ + T D   P I
Sbjct: 325 LEGNIVIDSGTTLTYFPVSYCNLVRQAVEHVVTAVRAADPTGNDMLCYNSDTIDI-FPVI 384

Query: 375 TMHFE-GADVPLSQENVFIRVSN-DVVCLAFSAGQENGIMLYGNIAQGNFLIGYDIKNMF 434
           TMHF  G D+ L + N+++  +N  V CLA          ++GN AQ NFL+GYD  ++ 
Sbjct: 385 TMHFSGGVDLVLDKYNMYMESNNGGVFCLAIICNSPTQEAIFGNRAQNNFLVGYDSSSLL 394

Query: 435 VSFKPTNCVAM 441
           VSF PTNC A+
Sbjct: 445 VSFSPTNCSAL 394

The following BLAST results are available for this feature:

BLAST of Lsi09G018790.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6XBF8	1.2e-114	49.18	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q3EBM5	3.0e-86	41.51	Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561...	[more]
Q766C3	8.6e-65	37.30	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	5.6e-64	35.58	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LNJ3	3.3e-48	36.39	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]

BLAST of Lsi09G018790.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E2N4	2.3e-169	69.16	aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC107991689 PE=3 SV=1	[more]
A0A0A0K9V4	3.0e-169	70.59	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G07863...	[more]
A0A5D3DLM9	6.6e-169	68.93	Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A0A0K928	5.8e-165	67.29	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G07865...	[more]
A0A5A7TAJ2	1.7e-161	69.10	Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of Lsi09G018790.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038907012.1	5.5e-186	77.41	aspartic proteinase CDR1-like [Benincasa hispida]	[more]
XP_038876324.1	8.3e-174	72.43	aspartic proteinase CDR1-like [Benincasa hispida]	[more]
XP_016902483.1	4.7e-169	69.16	PREDICTED: aspartic proteinase CDR1-like [Cucumis melo]	[more]
XP_031743830.1	6.1e-169	70.59	aspartic proteinase CDR1-like [Cucumis sativus] >KGN46268.1 hypothetical protein...	[more]
KAA0039977.1	1.4e-168	68.93	aspartic proteinase CDR1-like [Cucumis melo var. makuwa] >TYK24525.1 aspartic pr...	[more]

BLAST of Lsi09G018790.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G33340.1	8.4e-116	49.18	Eukaryotic aspartyl protease family protein	[more]
AT1G64830.1	1.8e-110	49.77	Eukaryotic aspartyl protease family protein	[more]
AT1G31450.1	2.1e-90	42.42	Eukaryotic aspartyl protease family protein	[more]
AT2G35615.1	2.2e-87	41.51	Eukaryotic aspartyl protease family protein	[more]
AT2G28010.1	7.4e-72	38.98	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 287..433 e-value: 4.2E-26 score: 91.7
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 51..249 e-value: 5.6E-51 score: 175.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 250..440 e-value: 1.7E-43 score: 150.4
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 70..437
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 76..252 e-value: 4.5E-51 score: 173.6
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 14..438
None	No IPR available	PANTHER	PTHR47967:SF66	ASPARTIC PROTEINASE CDR1-RELATED	coord: 14..438
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 76..433 score: 43.205212
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 75..437 e-value: 2.86402E-88 score: 267.592

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi09G018790	Lsi09G018790	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi09G018790.1.exon.4	Lsi09G018790.1.exon.4	exon
Lsi09G018790.1.exon.3	Lsi09G018790.1.exon.3	exon
Lsi09G018790.1.exon.2	Lsi09G018790.1.exon.2	exon
Lsi09G018790.1.exon.1	Lsi09G018790.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi09G018790.1.CDS.4	Lsi09G018790.1.CDS.4	CDS
Lsi09G018790.1.CDS.3	Lsi09G018790.1.CDS.3	CDS
Lsi09G018790.1.CDS.2	Lsi09G018790.1.CDS.2	CDS
Lsi09G018790.1.CDS.1	Lsi09G018790.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi09G018790.1	Lsi09G018790.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity