Lsi09G008870.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G008870.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCoA ligase
Locationchr09: 10415847 .. 10418362 (-)
Sequence length1812
RNA-Seq ExpressionLsi09G008870.1
SyntenyLsi09G008870.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTCTCTCTAACTCATAAATCTTTCAGCCTTTTGATTTTTCTTTTGTTCTTGTTCCTCTTATTTTATAAAACAAAAAATGGATATGCTTTTGAAATGTGATGCAAATTATGCTTCTCTTACACCTATCACCTTTCTTACGAGAGCTTCTGCATTCTACGCCGATCGAACTTCTATTATCTATGAGGGAACTCGCTTCACATGGCGTCAAACTTATGAGCGTTGTTGTCGTCTCGCTTCCTCCCTCTGTCGTCTCAACATCTCCAAAAACGACGTTGTAAGATCTTGCTCTCTTCTTCTTTTATTCTCTTCTCTCCTTCCTTCAAACATTTTTTTTTACTCTACATGAATCAACCCAAAAGTTTGAGCTTATGAAATATGGTAAATTTAATTATATTAACATTTTAACATTCCTTTCAAAATTTGTAGAAAGTCCAACAAGTGGAAATTAATCAATATTAATTGGAGAAGAAACGATATTATTTTAGGGGTTTTTTAACAAAGAATTACCTTATGCTCTTATATAATATTAGATTATCAAATTTAATTATATATATTGGTATTTTTACACTTAGAAATATATTTTAGGCTAAACATATGCTTTACTCTCTTGGTTCTTGAAGGTGTCAGTACTAGCACCAAATGTTCCAGCATTATACGAGATGCATTTTGCAGTACCGATGGCAGGTGCCGTTTTGAACACAATCAACACCCGCCTTGATGCCAATAACATCGCTGTCATCCTCCGTCACTCTGAGGCGAAAATCCTTTTTGTTGATTATCAATACATTAAGGAAGCAAAAGATGCCCTTCGTTTATTGGTGGCAGAATCCTTCCCAATCCCACTCGTTGTTTTCATTGATGATATTGACTCTCCCACAGGAGCTCGATTTGGAAACTTGGAATACGAGCAACTCATCCATGATGGTGATGTCAAGTTCACTCCGGTGGAGGTCCATGACGAATGGGACTCCATAGCACTAAATTACACCTCTGGCACGACTTCAGCACCAAAGGGCGTGGTGTATAGCCACCGTGGCGCGTTCCTTAGCACACTCAGTTTGGTAATGGGTTGGGAAATGGGAAATGCGCCTGTTTATTTGTGGACTCTACCGATGTTCCACTGTAACGGTTGGACCTTCACATGGGGGATCGCTGCTCGTGGTGGAACCAATGTTTGTATGCGCAATACTACCGCATTAGATATCTTTCGAAACATTAATTTGCATCACGTGACTCACATGTGTTGTGCACCGATCGTGTTCAGAATCATCCTCGATGCTGATAGCGGTAATGGACGTCGAGTTGTCTGGGTGGTGAACGTGTTGACTGGCGGAGCACCACCGCCAGCACCGTTGTTGGAAAAGATGGAGGCTCTTGGTTTCCATATAACACATGCTTATGGGCTAACAGAGGCCACTGGACCTGCGCTGGTTTGCGAATGGCAAGAAAAGTGGAATGTTTTGCCAGCCGATCAACAGGCCAAGCTAAAAGCTAGACAAGGTATTCTTTAATTTAATATATATATATATATATATATATATATATATATATATATATATATATATTTTTGAAACTTGTTAAACATTTTTTAAAAAATTAGAGATATATTAGATATAAAAATTAAAACTTCACAAATCTATTTGATGTGATTATGAAAATTTAATCAAGACTAATCTTATAAATTAAAATTAAAAACAAACTTAGACTTTGTGGAGAGATAGGTGATAGTTCTTTTTCATGCTTTTAGAACTTAGAAGTAAGAGTTAATGGTAAAATTTGAAAATCTAATTTTAATTTTTTAAAGGAAAACTTAATAAAAATAATTAACCAACTTTTGCACTATATATATCTAAATCAGGAATTAGCATTTTGACATTAGCCGATGTGGACGTCAAAAATTTGGACACCATGGAAAGTGTGCCGCACGACGGCCACACCTCCGGCGAGATTGTCCTACGTGGCAGCAGCCTTATGAAGGGCTACTTCAAAGATCCGGCAGCGACGGCGGCAGCCTTCAAAAACGGTTGGTTTCTAACTGGCGACATCGGTGTCATTTACCCCGACGGCTATTTAGAGATCAAGGATCGATCTAAGGACGTCATCATCTCTGGCGGTGAGAATATCAGCAGTGTCGAACTCGAGACGGTGCTCTACCGCCATCCACAGGTGGCGGAGGCAGCGGTTGTCGCAATGCCGCACCCTCGTTGGGGAGAGAGCCCTTGCGCCTTCGTCGCTGTCCATCGCGATGCTGAAAAAATAAGCGAGGCAGAGATCATTGCATTTTGCCGGAAGAATTTGTCGCACTATATGGTGCCAAAGAAAGTGGTGTTCGTGGCGGAGCTTCCAAAAACATCAACCGGAAAGATTCAAAAGTTCAAATTGAGGGAGCAGGCAAAATATTTTCAGGTGTCTGAGAAAAGAGTTGCCAGCACTAGCAAAAACAAATCCAGTCGAAAAGATGGTAGCCGACGATCGGATTATAGTGAGCATGTTTCGGCCATGTCTCGACTTTGA

mRNA sequence

TTTTCTCTCTAACTCATAAATCTTTCAGCCTTTTGATTTTTCTTTTGTTCTTGTTCCTCTTATTTTATAAAACAAAAAATGGATATGCTTTTGAAATGTGATGCAAATTATGCTTCTCTTACACCTATCACCTTTCTTACGAGAGCTTCTGCATTCTACGCCGATCGAACTTCTATTATCTATGAGGGAACTCGCTTCACATGGCGTCAAACTTATGAGCGTTGTTGTCGTCTCGCTTCCTCCCTCTGTCGTCTCAACATCTCCAAAAACGACGTTGTGTCAGTACTAGCACCAAATGTTCCAGCATTATACGAGATGCATTTTGCAGTACCGATGGCAGGTGCCGTTTTGAACACAATCAACACCCGCCTTGATGCCAATAACATCGCTGTCATCCTCCGTCACTCTGAGGCGAAAATCCTTTTTGTTGATTATCAATACATTAAGGAAGCAAAAGATGCCCTTCGTTTATTGGTGGCAGAATCCTTCCCAATCCCACTCGTTGTTTTCATTGATGATATTGACTCTCCCACAGGAGCTCGATTTGGAAACTTGGAATACGAGCAACTCATCCATGATGGTGATGTCAAGTTCACTCCGGTGGAGGTCCATGACGAATGGGACTCCATAGCACTAAATTACACCTCTGGCACGACTTCAGCACCAAAGGGCGTGGTGTATAGCCACCGTGGCGCGTTCCTTAGCACACTCAGTTTGGTAATGGGTTGGGAAATGGGAAATGCGCCTGTTTATTTGTGGACTCTACCGATGTTCCACTGTAACGGTTGGACCTTCACATGGGGGATCGCTGCTCGTGGTGGAACCAATGTTTGTATGCGCAATACTACCGCATTAGATATCTTTCGAAACATTAATTTGCATCACGTGACTCACATGTGTTGTGCACCGATCGTGTTCAGAATCATCCTCGATGCTGATAGCGGTAATGGACGTCGAGTTGTCTGGGTGGTGAACGTGTTGACTGGCGGAGCACCACCGCCAGCACCGTTGTTGGAAAAGATGGAGGCTCTTGGTTTCCATATAACACATGCTTATGGGCTAACAGAGGCCACTGGACCTGCGCTGGTTTGCGAATGGCAAGAAAAGTGGAATGTTTTGCCAGCCGATCAACAGGCCAAGCTAAAAGCTAGACAAGGAATTAGCATTTTGACATTAGCCGATGTGGACGTCAAAAATTTGGACACCATGGAAAGTGTGCCGCACGACGGCCACACCTCCGGCGAGATTGTCCTACGTGGCAGCAGCCTTATGAAGGGCTACTTCAAAGATCCGGCAGCGACGGCGGCAGCCTTCAAAAACGGTTGGTTTCTAACTGGCGACATCGGTGTCATTTACCCCGACGGCTATTTAGAGATCAAGGATCGATCTAAGGACGTCATCATCTCTGGCGGTGAGAATATCAGCAGTGTCGAACTCGAGACGGTGCTCTACCGCCATCCACAGGTGGCGGAGGCAGCGGTTGTCGCAATGCCGCACCCTCGTTGGGGAGAGAGCCCTTGCGCCTTCGTCGCTGTCCATCGCGATGCTGAAAAAATAAGCGAGGCAGAGATCATTGCATTTTGCCGGAAGAATTTGTCGCACTATATGGTGCCAAAGAAAGTGGTGTTCGTGGCGGAGCTTCCAAAAACATCAACCGGAAAGATTCAAAAGTTCAAATTGAGGGAGCAGGCAAAATATTTTCAGGTGTCTGAGAAAAGAGTTGCCAGCACTAGCAAAAACAAATCCAGTCGAAAAGATGGTAGCCGACGATCGGATTATAGTGAGCATGTTTCGGCCATGTCTCGACTTTGA

Coding sequence (CDS)

ATGGATATGCTTTTGAAATGTGATGCAAATTATGCTTCTCTTACACCTATCACCTTTCTTACGAGAGCTTCTGCATTCTACGCCGATCGAACTTCTATTATCTATGAGGGAACTCGCTTCACATGGCGTCAAACTTATGAGCGTTGTTGTCGTCTCGCTTCCTCCCTCTGTCGTCTCAACATCTCCAAAAACGACGTTGTGTCAGTACTAGCACCAAATGTTCCAGCATTATACGAGATGCATTTTGCAGTACCGATGGCAGGTGCCGTTTTGAACACAATCAACACCCGCCTTGATGCCAATAACATCGCTGTCATCCTCCGTCACTCTGAGGCGAAAATCCTTTTTGTTGATTATCAATACATTAAGGAAGCAAAAGATGCCCTTCGTTTATTGGTGGCAGAATCCTTCCCAATCCCACTCGTTGTTTTCATTGATGATATTGACTCTCCCACAGGAGCTCGATTTGGAAACTTGGAATACGAGCAACTCATCCATGATGGTGATGTCAAGTTCACTCCGGTGGAGGTCCATGACGAATGGGACTCCATAGCACTAAATTACACCTCTGGCACGACTTCAGCACCAAAGGGCGTGGTGTATAGCCACCGTGGCGCGTTCCTTAGCACACTCAGTTTGGTAATGGGTTGGGAAATGGGAAATGCGCCTGTTTATTTGTGGACTCTACCGATGTTCCACTGTAACGGTTGGACCTTCACATGGGGGATCGCTGCTCGTGGTGGAACCAATGTTTGTATGCGCAATACTACCGCATTAGATATCTTTCGAAACATTAATTTGCATCACGTGACTCACATGTGTTGTGCACCGATCGTGTTCAGAATCATCCTCGATGCTGATAGCGGTAATGGACGTCGAGTTGTCTGGGTGGTGAACGTGTTGACTGGCGGAGCACCACCGCCAGCACCGTTGTTGGAAAAGATGGAGGCTCTTGGTTTCCATATAACACATGCTTATGGGCTAACAGAGGCCACTGGACCTGCGCTGGTTTGCGAATGGCAAGAAAAGTGGAATGTTTTGCCAGCCGATCAACAGGCCAAGCTAAAAGCTAGACAAGGAATTAGCATTTTGACATTAGCCGATGTGGACGTCAAAAATTTGGACACCATGGAAAGTGTGCCGCACGACGGCCACACCTCCGGCGAGATTGTCCTACGTGGCAGCAGCCTTATGAAGGGCTACTTCAAAGATCCGGCAGCGACGGCGGCAGCCTTCAAAAACGGTTGGTTTCTAACTGGCGACATCGGTGTCATTTACCCCGACGGCTATTTAGAGATCAAGGATCGATCTAAGGACGTCATCATCTCTGGCGGTGAGAATATCAGCAGTGTCGAACTCGAGACGGTGCTCTACCGCCATCCACAGGTGGCGGAGGCAGCGGTTGTCGCAATGCCGCACCCTCGTTGGGGAGAGAGCCCTTGCGCCTTCGTCGCTGTCCATCGCGATGCTGAAAAAATAAGCGAGGCAGAGATCATTGCATTTTGCCGGAAGAATTTGTCGCACTATATGGTGCCAAAGAAAGTGGTGTTCGTGGCGGAGCTTCCAAAAACATCAACCGGAAAGATTCAAAAGTTCAAATTGAGGGAGCAGGCAAAATATTTTCAGGTGTCTGAGAAAAGAGTTGCCAGCACTAGCAAAAACAAATCCAGTCGAAAAGATGGTAGCCGACGATCGGATTATAGTGAGCATGTTTCGGCCATGTCTCGACTTTGA

Protein sequence

MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLNISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQYIKEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVEVHDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVWVVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLKARQGISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGWFLTGDIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESPCAFVAVHRDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKYFQVSEKRVASTSKNKSSRKDGSRRSDYSEHVSAMSRL
Homology
[Close]
BLAST of Lsi09G008870.1 vs. ExPASy Swiss-Prot
Match: I3PB36 (Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 2.0e-215
Identity = 367/554 (66.25%), Postives = 429/554 (77.44%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           MD L KC ANY  LTP+TFLTRA   YA+RTSIIY G RFTW QTY+RCCRLASSL  LN
Sbjct: 1   MDELPKCGANYVPLTPLTFLTRAFKSYANRTSIIYAGARFTWEQTYKRCCRLASSLQSLN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           I KNDVVSVLAPNVPA YEMHFAVPMAGAVLNTINTRLD  NIA+IL+HSEAK+LFVDY+
Sbjct: 61  IVKNDVVSVLAPNVPATYEMHFAVPMAGAVLNTINTRLDPMNIAIILKHSEAKLLFVDYE 120

Query: 121 YIKEAKDALRLLVAESF---------PIPLVVFIDDIDSPTG-ARFGNLEYEQLIHDGDV 180
           Y+++A+ AL LL++ +F          +P V+ IDD+ SPT   +   LEYEQL+H G+ 
Sbjct: 121 YLEKARKALELLMSTNFITAQNSKKISMPQVILIDDLYSPTRIQQQDQLEYEQLVHQGNP 180

Query: 181 KFTPVE-VHDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTL 240
           ++ P   V DEWD I LNYTSGTTS PKGVVYSHRGAFLSTL+ +MGWEMG  PVYLW+L
Sbjct: 181 EYAPENIVDDEWDPIVLNYTSGTTSEPKGVVYSHRGAFLSTLNTIMGWEMGTEPVYLWSL 240

Query: 241 PMFHCNGWTFTWGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSG 300
           PMFH NGWT TWGIAARGGTNVC+RNTTA +I+ NI LH VTHMCCAP VF I+L+A   
Sbjct: 241 PMFHINGWTLTWGIAARGGTNVCIRNTTAQEIYSNITLHKVTHMCCAPTVFNILLEAKPH 300

Query: 301 NGRRVVWVVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPA 360
             R +   V V+ GGAPPP  L+ K+E LGFH+ H YG+TEA G  LVCEWQ +WN L  
Sbjct: 301 ERREITTPVQVMVGGAPPPTTLIGKIEELGFHVVHCYGITEAGGTTLVCEWQSEWNKLSR 360

Query: 361 DQQAKLKARQGISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATA 420
           + QA LKARQGIS+L L DVDVKN  TM+SVPH+G T GEI LRGSS+MKGYFK+  A +
Sbjct: 361 EDQANLKARQGISVLALEDVDVKNSKTMQSVPHNGKTMGEICLRGSSIMKGYFKNDKANS 420

Query: 421 AAFKNGWFLTGDIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVV 480
             FKNGWFLTGD+ VI+ DGYLEIKDR KD+IISGGENISS+E+E  + +HP V EAAVV
Sbjct: 421 QVFKNGWFLTGDVAVIHQDGYLEIKDRCKDIIISGGENISSIEVENAILKHPSVIEAAVV 480

Query: 481 AMPHPRWGESPCAFVAVHRDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKI 540
           AMPHPRWGE+PCAFV   ++ E I EA+II  C+K L  +MVPK V F+ ELPKT TGK+
Sbjct: 481 AMPHPRWGETPCAFVIKTKNPE-IKEADIIVHCKKELPGFMVPKHVQFLEELPKTGTGKV 540

Query: 541 QKFKLREQAKYFQV 544
           +K +LRE AK F +
Sbjct: 541 KKLQLREMAKSFGI 553

[Close]
BLAST of Lsi09G008870.1 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 2.0e-199
Identity = 349/571 (61.12%), Postives = 427/571 (74.78%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           MD L+ C+AN   LTPITFL RAS  Y +RTSIIY  TRFTW QTY+RCCRLA+SL  LN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           I++NDVVS+LAPNVPA+YEMHF+VPM GAVLN INTRLDA  IA+ILRH+E KILFVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YIKEAKDALRLL-VAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGD----VKFTPV 180
           +    ++ LRL+   +S P P ++ I++IDS T      L+YE LI  G+       +  
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 EVHDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            VH+E D I+LNYTSGTT+ PKGVV SH+GA+LS LS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVV 300
           GWT TW +AARGGTNVC+R+ TA +I++NI LH VTHM C P VFR +L+    +     
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 WVVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKL 360
             V VLTGG+ PPA L++K+E LGFH+ H YGLTEATGP L CEWQ++WN LP  QQ +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNG 420
           + RQG+  LTLADVDVKN  T+ESVP DG T GEIV++GSSLMKGY K+P AT+ AFK+G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420

Query: 421 WFLTGDIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGDIGVI+PDGY+EIKDRSKD+IISGGENISS+E+E VLY + +V EAAVVAMPHP 
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480

Query: 481 WGESPCAFVAVHRDAEKI--SEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFK 540
           WGE+PCAFV + +  E +  SE ++I +CR+N+ H+M PKKVVF  ELPK S GKI K K
Sbjct: 481 WGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSK 540

Query: 541 LREQAKYFQVSEKRVASTSKNKSSRKDGSRR 565
           LR+ AK   V E    S   ++ S +  S R
Sbjct: 541 LRDIAKALVVREDDAGSKKVHQRSIEHVSSR 571

[Close]
BLAST of Lsi09G008870.1 vs. ExPASy Swiss-Prot
Match: Q9SS01 (Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 2.3e-198
Identity = 346/561 (61.68%), Postives = 424/561 (75.58%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           MD L  C+AN   LTP+TFL RAS  Y +RTSIIY  TRFTW QTY+RCCRLA+SL  LN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           ISKNDVVSV+APN PALYEMHFAVPMAGAVLN INTRLDA +IA ILRH++ KILF+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YIKEAKDALRLLVAESFPIPL-VVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPV---- 180
           +   A+++L LL +E   + L V+FI + D P  A F  L+YE LI  G+   + V    
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180

Query: 181 EVHDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + DE D I+LNYTSGTT+ PKGVV SHRGA+L TLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVV 300
           GWTFTWG AARGGT+VCMR+ TA +I++NI +H+VTHMCC P VF I+L  +S +     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300

Query: 301 WVVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKL 360
             V+VLTGG+PPPA L++K++ LGF + HAYG TEATGP L CEWQ++WN LP +QQ +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNG 420
           KARQGISIL LADVDVKN +T +S P DG T GEI+++GSS+MKGY K+P AT  AFK+G
Sbjct: 361 KARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKHG 420

Query: 421 WFLTGDIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGD+GVI+PDG++EIKDRSKD+IISGGENISSVE+E VLY++P+V E AVVAMPHP 
Sbjct: 421 WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAVHR----------DAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTS 540
           WGE+PCAFV + +          D  +  E  +I +CR+NL H+M P+KVVF+ ELPK  
Sbjct: 481 WGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNG 540

Query: 541 TGKIQKFKLREQAKYFQVSEK 547
            GKI K KLR+ AK   V ++
Sbjct: 541 NGKILKPKLRDIAKGLVVEDE 561

[Close]
BLAST of Lsi09G008870.1 vs. ExPASy Swiss-Prot
Match: Q9SS00 (Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE12 PE=2 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 8.0e-196
Identity = 345/569 (60.63%), Postives = 426/569 (74.87%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           MD L  C+AN   LTPITFL RAS  Y +RTSIIY  TRFTW QTY+RCCRLA+SL  LN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           I KNDVVSV+APN PA+YEMHFAVPMAGAVLN INTRLDA +IA ILRH++ KILF+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 YIKEAKDALRLLVAESFPIPL-VVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVE--- 180
           +   A++ L+LL +E   + L V+FI +ID P        +YE LI  G+   TP+    
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEP--TPLLLAR 180

Query: 181 ---VHDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFH 240
              + DE D I+LNYTSGTT+ PKGVV SHRGA+LSTLS ++GWEMG  PVYLWTLPMFH
Sbjct: 181 MFCIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFH 240

Query: 241 CNGWTFTWGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRR 300
           CNGWTFTWG AARGGT+VCMR+ TA +I++NI +H+VTHMCC P VF I+L  +S +   
Sbjct: 241 CNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSH 300

Query: 301 VVWVVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQA 360
               V+VLTGG+PPPA L++K++ LGF + HAYGLTEATGP L CEWQ++WN LP +QQ 
Sbjct: 301 RSGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQM 360

Query: 361 KLKARQGISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFK 420
           +LKARQG+SIL L +VDV+N +T ESVP DG T GEIV++GSS+MKGY K+P AT  AFK
Sbjct: 361 ELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFK 420

Query: 421 NGWFLTGDIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPH 480
           +GW  +GD+GVI+PDG++EIKDRSKD+IISGGENISSVE+E ++Y++P+V E AVVAMPH
Sbjct: 421 HGWLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPH 480

Query: 481 PRWGESPCAFVAVHR---------DAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKT 540
           P WGE+PCAFV + +         D     E ++I +CR+NL H+M P+KVVF+ ELPK 
Sbjct: 481 PTWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKN 540

Query: 541 STGKIQKFKLREQAKYFQVSEKRVASTSK 554
             GKI K KLR+ AK   V+E  V   SK
Sbjct: 541 GNGKILKPKLRDIAKGL-VAEDEVNVRSK 566

[Close]
BLAST of Lsi09G008870.1 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.7e-166
Identity = 284/543 (52.30%), Postives = 391/543 (72.01%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+  +K  ANY  LTPI+FL R++  YADR SI+Y   ++TWRQT +RC R+AS+L +L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           IS  DVVSVLAPNVPA+ E+HF VPMAGA+L T+N R D++ +AV+LRHS  K++F D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YIKEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGN----LEYEQLIHDGDVKFTPVE 180
           +++ A+ A  +L  +   +P++V I +  + + +R       +EYE ++  G   F  + 
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 VHDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
             DE D+I++NYTSGTTS+PKGVVYSHRGA+L++L+ V+  EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFTWGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVW 300
           W   WG+ A GGTN+C+RN TA  IF NI+ H VTHM  AP +  +I++A     + +  
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 VVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLK 360
            V+ +TG APPPA ++ KME LGF + H+YGLTE  GP  +C W+ +W+ LP ++QAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 ARQGISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGW 420
           ARQG++ L L ++ VK+  TM ++P DG T GE+V RG+++M GY K+P AT  AFK GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FLTGDIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRW 480
           F +GD+GV +PDGY+E+KDRSKD+IISGGENISS+E+E+ L+ HP V EAAVVA P   W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GESPCAFVAVHRDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLRE 540
           GE+ CAFV + +D  K S  E+I++CR  L HYM P+ +VF  +LPKTSTGK+QKF LR 
Sbjct: 483 GETACAFVKL-KDGSKASAEELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKFVLRT 542

[Close]
BLAST of Lsi09G008870.1 vs. ExPASy TrEMBL
Match: A0A6J1ECD9 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431279 PE=4 SV=1)

HSP 1 Score: 1027.7 bits (2656), Expect = 1.9e-296
Identity = 501/577 (86.83%), Postives = 533/577 (92.37%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+ L KCDANYASLTPITFL RASAFYADRTSIIYEGTRFTWRQTY+RCCRLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           ++KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD NNIA+ILRHSEAKILFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIKEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVEVHDE 180
           +I+EAKDALR L+A S P+P VV IDDIDSPTGARFG LEYEQL+HDGDV FTPVEV DE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVWVVNV 300
           WGIAARGGTNVCMRNTTA DI+RNINLHHVTHMCCAPIVFRIIL+A+ G  RR  W+VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPAD+QAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDG T+GEIVLRGSS+MKGYFKDPAATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVHRDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKY 540
           CAFV VH D  K+SEAE+I FCRKN+SHYM PKKVVFVAELPKT TGKIQKFKLREQAK 
Sbjct: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 FQVSEKRVASTSKNKSSRKDGSRRSDYSEHVSAMSRL 578
              SEKRV  +  NKS R DG R+ DY +HV AMSRL
Sbjct: 541 LVGSEKRV--SGGNKSGRHDGGRQPDYGQHVMAMSRL 575

[Close]
BLAST of Lsi09G008870.1 vs. ExPASy TrEMBL
Match: A0A0A0KT40 (Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G319910 PE=4 SV=1)

HSP 1 Score: 1025.0 bits (2649), Expect = 1.2e-295
Identity = 506/577 (87.69%), Postives = 534/577 (92.55%), Query Frame = 0 

Query: 3   MLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLNIS 62
           MLLKCDANY++LTPITFL RASAFYADRTSIIYEGTRFTW+QTYERCCRLASSLCRL +S
Sbjct: 1   MLLKCDANYSALTPITFLKRASAFYADRTSIIYEGTRFTWKQTYERCCRLASSLCRLTVS 60

Query: 63  KNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQYI 122
           KNDVVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD  NIA+ILRHSEAKI FVDYQYI
Sbjct: 61  KNDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDIKNIALILRHSEAKIFFVDYQYI 120

Query: 123 KEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVEVHDEWD 182
           +EAKDALRLLVAES PIPLVV IDDID+PTGAR GNLEYEQLI+DGD  FTPVEV DEWD
Sbjct: 121 QEAKDALRLLVAESSPIPLVVVIDDIDTPTGARLGNLEYEQLIYDGDANFTPVEVDDEWD 180

Query: 183 SIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWG 242
           SIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWG
Sbjct: 181 SIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWG 240

Query: 243 IAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVWVVNVLT 302
           IAARGGTN+CMRNTTA DIFRNINLH VTHMCCAPIVF IIL+AD+ + RRV W VNVLT
Sbjct: 241 IAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFTIILEADNRDRRRVAWPVNVLT 300

Query: 303 GGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLKARQGIS 362
           GGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQA LKARQGIS
Sbjct: 301 GGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQGIS 360

Query: 363 ILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGWFLTGDI 422
           ILTLADVDVKNL TMESVPHDG T+GEIVLRGSSLMKGYFKDPAATAAA KNGWFLTGDI
Sbjct: 361 ILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPAATAAALKNGWFLTGDI 420

Query: 423 GVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESPCA 482
           GVIYPDGY+EIKDRSKDVIISGGENISSVE+ETVLYRHPQV+EAAVVAMPHPRWGESPCA
Sbjct: 421 GVIYPDGYVEIKDRSKDVIISGGENISSVEVETVLYRHPQVSEAAVVAMPHPRWGESPCA 480

Query: 483 FVAVH--RDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKY 542
           FV +     + ++SEAE++AFCRKNLSHYMVPKKVVFVAELPK + GK+QKF LREQAKY
Sbjct: 481 FVVLQDGGGSGRVSEAEMVAFCRKNLSHYMVPKKVVFVAELPK-ANGKVQKFMLREQAKY 540

Query: 543 FQVSEKRVASTSKNKSSRKDGSRRSDYSEHVSAMSRL 578
            +VSEKR+ S   NKS    G+RRSDY EHV AMSRL
Sbjct: 541 LEVSEKRIVSNMGNKS----GNRRSDYGEHVIAMSRL 572

[Close]
BLAST of Lsi09G008870.1 vs. ExPASy TrEMBL
Match: A0A5A7TXW0 (Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold284G00160 PE=4 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.7e-294
Identity = 509/580 (87.76%), Postives = 534/580 (92.07%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+MLLKCDANY +LTPITFL RASAFYADRTSIIYEGTRFTW QTYERCCRLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           +SKN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD  NIA+ILRHSEAKI FVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIKEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVEVHDE 180
           YI+EAKDALRLLVAESF IPLVV IDDID+PTGAR GNLEYEQLIHDGD  FTPVEV DE
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVWVVNV 300
           WGIAARGGTN+CMRNTTA DIFRNINLH VTHMCCAPIVFRIIL+AD+G+ RRV W VNV
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQA LKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGWFLTG 420
           ISILTLADVDVKNL TMESVPHDG T+GEIVLRGSSLMKGYFKDP ATAAAFKNGWFLTG
Sbjct: 361 ISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLTG 420

Query: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DIGVIYPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVH--RDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQA 540
           CAFV +H    + ++SEAE+IAFCRKNLSHYMVPKKVVF+AELPK + GK+QKF LREQA
Sbjct: 481 CAFVVLHGGGGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPK-ANGKVQKFMLREQA 540

Query: 541 KYFQVSEKRVASTSKNKSSRKDGSRRSDY-SEHVSAMSRL 578
           KY +VSEKR  S   NKS    G+RRSDY  +HV AMSRL
Sbjct: 541 KYLEVSEKRRVSNLGNKS----GNRRSDYGDQHVIAMSRL 575

[Close]
BLAST of Lsi09G008870.1 vs. ExPASy TrEMBL
Match: A0A1S3CIM1 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103501376 PE=4 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.7e-294
Identity = 509/580 (87.76%), Postives = 534/580 (92.07%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+MLLKCDANY +LTPITFL RASAFYADRTSIIYEGTRFTW QTYERCCRLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           +SKN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD  NIA+ILRHSEAKI FVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIKEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVEVHDE 180
           YI+EAKDALRLLVAESF IPLVV IDDID+PTGAR GNLEYEQLIHDGD  FTPVEV DE
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVWVVNV 300
           WGIAARGGTN+CMRNTTA DIFRNINLH VTHMCCAPIVFRIIL+AD+G+ RRV W VNV
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQA LKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGWFLTG 420
           ISILTLADVDVKNL TMESVPHDG T+GEIVLRGSSLMKGYFKDP ATAAAFKNGWFLTG
Sbjct: 361 ISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLTG 420

Query: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DIGVIYPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVH--RDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQA 540
           CAFV +H    + ++SEAE+IAFCRKNLSHYMVPKKVVF+AELPK + GK+QKF LREQA
Sbjct: 481 CAFVVLHGGGGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPK-ANGKVQKFMLREQA 540

Query: 541 KYFQVSEKRVASTSKNKSSRKDGSRRSDY-SEHVSAMSRL 578
           KY +VSEKR  S   NKS    G+RRSDY  +HV AMSRL
Sbjct: 541 KYLEVSEKRRVSNLGNKS----GNRRSDYGDQHVIAMSRL 575

[Close]
BLAST of Lsi09G008870.1 vs. ExPASy TrEMBL
Match: A0A6J1JUR4 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111487678 PE=4 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 2.5e-293
Identity = 496/577 (85.96%), Postives = 530/577 (91.85%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+ L KCDAN+ASLTPITFL RASAFYADRTSIIYEGT FTWRQTYERCC LASSL RLN
Sbjct: 1   MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           + KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD +NIA+ILRHSEAKILFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIKEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVEVHDE 180
           +I+EAKDALR L+A S P+P VV IDDIDSPTGARFG LEYEQL+HDGDV FTPVEV DE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVWVVNV 300
           WGIAARGGTNVC+RNTTA DI+RNINLHHVTHMCCAPIVFRIIL+A+ G  RR  W+VNV
Sbjct: 241 WGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPAD+QAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDG T+GEIVLRGSS+MKGYFKDPAATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVHRDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKY 540
           CAFV VH D+ K+SEAE+I FCRKN+SHYM PKKV+FVAELPKT TGKIQKFKLREQAK 
Sbjct: 481 CAFVVVHSDSGKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 FQVSEKRVASTSKNKSSRKDGSRRSDYSEHVSAMSRL 578
              SEKRV  +  NKS R DG R  +Y EHV AMSRL
Sbjct: 541 LVASEKRV--SGGNKSGRHDGGRLPEYGEHVMAMSRL 575

[Close]
BLAST of Lsi09G008870.1 vs. NCBI nr
Match: XP_038906606.1 (trans-cinnamate:CoA ligase, peroxisomal-like [Benincasa hispida])

HSP 1 Score: 1067.8 bits (2760), Expect = 3.4e-308
Identity = 523/577 (90.64%), Postives = 544/577 (94.28%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           MDMLLKCDANY SLTPITFLTRAS FYADRTSIIYEGTRFTW QTYERCCRLASSL  LN
Sbjct: 1   MDMLLKCDANYTSLTPITFLTRASTFYADRTSIIYEGTRFTWHQTYERCCRLASSLRHLN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           ISKNDVVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD NNIAVILRHSEAKILFVDYQ
Sbjct: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIAVILRHSEAKILFVDYQ 120

Query: 121 YIKEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVEVHDE 180
           YI+EAKDALRLL+A+SFP+PLVV IDDIDSPTG RFGN EYE+LIHDGDV FTP+EV DE
Sbjct: 121 YIQEAKDALRLLMAKSFPMPLVVIIDDIDSPTGIRFGNFEYEKLIHDGDVNFTPLEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVWVVNV 300
           WGIAARGGTN+CMRNTTA+DIFRNINLHHVTHMCCAPIVFRIILDA+S +  RV W +NV
Sbjct: 241 WGIAARGGTNICMRNTTAIDIFRNINLHHVTHMCCAPIVFRIILDANSHDRCRVAWPINV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLKARQG 360
           LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN LPAD+QAKLKARQG
Sbjct: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNTLPADKQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGWFLTG 420
           ISILTLADVDVKNLDTMESVPHDGHT+GEIVLRGS LMKGYFKDP ATAAAFKNGWFLTG
Sbjct: 361 ISILTLADVDVKNLDTMESVPHDGHTTGEIVLRGSGLMKGYFKDPVATAAAFKNGWFLTG 420

Query: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DIGVIYPDGYLEIKDRSKDVIISGGENISSVE+ETVLY HPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYSHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVHRDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKY 540
           CAFVAVH DA ++SEAEIIAFCRKNL HYMVPKKVVFV ELPK + GKIQKFKLREQAKY
Sbjct: 481 CAFVAVHGDAGRVSEAEIIAFCRKNLPHYMVPKKVVFVGELPK-ANGKIQKFKLREQAKY 540

Query: 541 FQVSEKRVASTSKNKSSRKDGSRRSDYSEHVSAMSRL 578
            +VSEKRV  T  NKSSR+DG+RRSDY EHV AMSRL
Sbjct: 541 LEVSEKRVVGTGWNKSSRQDGNRRSDYGEHVLAMSRL 576

[Close]
BLAST of Lsi09G008870.1 vs. NCBI nr
Match: XP_038881152.1 (LOW QUALITY PROTEIN: trans-cinnamate:CoA ligase, peroxisomal-like [Benincasa hispida])

HSP 1 Score: 1043.1 bits (2696), Expect = 8.8e-301
Identity = 513/577 (88.91%), Postives = 534/577 (92.55%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           MDMLLKCD NY SLTPITFLTRAS FYADRTSIIYEGTRFTW QTYER           N
Sbjct: 1   MDMLLKCDXNYTSLTPITFLTRASTFYADRTSIIYEGTRFTWHQTYERVVASPPPSAISN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           ISKNDVVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD NNIAVILRHSEAKILFVDYQ
Sbjct: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIAVILRHSEAKILFVDYQ 120

Query: 121 YIKEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVEVHDE 180
           YI+EAKDALRLL+A+SFP+PLVV IDDIDSPTG RFGN EYE+LIHDGDV FTP+EV DE
Sbjct: 121 YIQEAKDALRLLMAKSFPMPLVVIIDDIDSPTGIRFGNFEYEKLIHDGDVNFTPLEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVWVVNV 300
           WGIAARGGTN+CMRNTTA+DIFRNINLHHVTHMCCAPIVFRIILDA+S +  RV W +NV
Sbjct: 241 WGIAARGGTNICMRNTTAIDIFRNINLHHVTHMCCAPIVFRIILDANSHDRCRVAWPINV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLKARQG 360
           LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN LPAD+QAKLKARQG
Sbjct: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNTLPADKQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGWFLTG 420
           ISILTLADVDVKNLDTMESVPHDGHT+GEIVLRGS LMKGYFKDP ATAAAFKNGWFLTG
Sbjct: 361 ISILTLADVDVKNLDTMESVPHDGHTTGEIVLRGSGLMKGYFKDPVATAAAFKNGWFLTG 420

Query: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DIGVIYPDGYLEIKDRSKDVIISGGENISSVE+ETVLY HPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYSHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVHRDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKY 540
           CAFVAVH DA ++SEAEIIAFCRKNL HYMVPKKVVFV ELPK + GKIQKFKLREQAKY
Sbjct: 481 CAFVAVHGDAGRVSEAEIIAFCRKNLPHYMVPKKVVFVGELPK-ANGKIQKFKLREQAKY 540

Query: 541 FQVSEKRVASTSKNKSSRKDGSRRSDYSEHVSAMSRL 578
            +VSEKRV  T  NKSSR+DG+RRSDY EHV AMSRL
Sbjct: 541 LEVSEKRVVGTGWNKSSRQDGNRRSDYGEHVLAMSRL 576

[Close]
BLAST of Lsi09G008870.1 vs. NCBI nr
Match: XP_011655134.1 (butyrate--CoA ligase AAE11, peroxisomal [Cucumis sativus] >KGN51049.2 hypothetical protein Csa_023114 [Cucumis sativus])

HSP 1 Score: 1027.7 bits (2656), Expect = 3.8e-296
Identity = 507/579 (87.56%), Postives = 536/579 (92.57%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+MLLKCDANY++LTPITFL RASAFYADRTSIIYEGTRFTW+QTYERCCRLASSLCRL 
Sbjct: 1   MEMLLKCDANYSALTPITFLKRASAFYADRTSIIYEGTRFTWKQTYERCCRLASSLCRLT 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           +SKNDVVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD  NIA+ILRHSEAKI FVDYQ
Sbjct: 61  VSKNDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDIKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIKEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVEVHDE 180
           YI+EAKDALRLLVAES PIPLVV IDDID+PTGAR GNLEYEQLI+DGD  FTPVEV DE
Sbjct: 121 YIQEAKDALRLLVAESSPIPLVVVIDDIDTPTGARLGNLEYEQLIYDGDANFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVWVVNV 300
           WGIAARGGTN+CMRNTTA DIFRNINLH VTHMCCAPIVF IIL+AD+ + RRV W VNV
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFTIILEADNRDRRRVAWPVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLKARQG 360
           LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQA LKARQG
Sbjct: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGWFLTG 420
           ISILTLADVDVKNL TMESVPHDG T+GEIVLRGSSLMKGYFKDPAATAAA KNGWFLTG
Sbjct: 361 ISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPAATAAALKNGWFLTG 420

Query: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DIGVIYPDGY+EIKDRSKDVIISGGENISSVE+ETVLYRHPQV+EAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYVEIKDRSKDVIISGGENISSVEVETVLYRHPQVSEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVH--RDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQA 540
           CAFV +     + ++SEAE++AFCRKNLSHYMVPKKVVFVAELPK + GK+QKF LREQA
Sbjct: 481 CAFVVLQDGGGSGRVSEAEMVAFCRKNLSHYMVPKKVVFVAELPK-ANGKVQKFMLREQA 540

Query: 541 KYFQVSEKRVASTSKNKSSRKDGSRRSDYSEHVSAMSRL 578
           KY +VSEKR+ S   NKS    G+RRSDY EHV AMSRL
Sbjct: 541 KYLEVSEKRIVSNMGNKS----GNRRSDYGEHVIAMSRL 574

[Close]
BLAST of Lsi09G008870.1 vs. NCBI nr
Match: XP_022923630.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 1027.7 bits (2656), Expect = 3.8e-296
Identity = 501/577 (86.83%), Postives = 533/577 (92.37%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+ L KCDANYASLTPITFL RASAFYADRTSIIYEGTRFTWRQTY+RCCRLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           ++KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD NNIA+ILRHSEAKILFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIKEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVEVHDE 180
           +I+EAKDALR L+A S P+P VV IDDIDSPTGARFG LEYEQL+HDGDV FTPVEV DE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVWVVNV 300
           WGIAARGGTNVCMRNTTA DI+RNINLHHVTHMCCAPIVFRIIL+A+ G  RR  W+VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPAD+QAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDG T+GEIVLRGSS+MKGYFKDPAATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVHRDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKY 540
           CAFV VH D  K+SEAE+I FCRKN+SHYM PKKVVFVAELPKT TGKIQKFKLREQAK 
Sbjct: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 FQVSEKRVASTSKNKSSRKDGSRRSDYSEHVSAMSRL 578
              SEKRV  +  NKS R DG R+ DY +HV AMSRL
Sbjct: 541 LVGSEKRV--SGGNKSGRHDGGRQPDYGQHVMAMSRL 575

[Close]
BLAST of Lsi09G008870.1 vs. NCBI nr
Match: KAG6602004.1 (Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1023.8 bits (2646), Expect = 5.6e-295
Identity = 500/577 (86.66%), Postives = 532/577 (92.20%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+ L KCDANYASLTPITFL RASAFYADRTSIIYEGTRFTWRQTY+RCCRLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           ++KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD NNIA+ILRHSEAKILFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIKEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVEVHDE 180
           +I+EAKDALR L+A S P+P VV IDDIDSPTGARFG LEYEQL+HDGDV FTPVEV DE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVWVVNV 300
           WGIAARGGTNVCMRNTTA DI+RNINLHHVTHMCCAPIVFRIIL+A+ G  RR  W+VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPAD+QAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDG T+GEIVLRGSS+MKGYFKD AATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDLAATAKAFQNGWFLTG 420

Query: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVHRDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKY 540
           CAFV VH D  K+SEAE+I FCRKN+SHYM PKKVVFVAELPKT TGKIQKFKLREQAK 
Sbjct: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 FQVSEKRVASTSKNKSSRKDGSRRSDYSEHVSAMSRL 578
              SEKRV  +  NKS R DG R+ DY +HV AMSRL
Sbjct: 541 LVGSEKRV--SGGNKSGRHDGGRQPDYGQHVMAMSRL 575

[Close]
BLAST of Lsi09G008870.1 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 696.8 bits (1797), Expect = 1.4e-200
Identity = 349/571 (61.12%), Postives = 427/571 (74.78%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           MD L+ C+AN   LTPITFL RAS  Y +RTSIIY  TRFTW QTY+RCCRLA+SL  LN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           I++NDVVS+LAPNVPA+YEMHF+VPM GAVLN INTRLDA  IA+ILRH+E KILFVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YIKEAKDALRLL-VAESFPIPLVVFIDDIDSPTGARFGNLEYEQLIHDGD----VKFTPV 180
           +    ++ LRL+   +S P P ++ I++IDS T      L+YE LI  G+       +  
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 EVHDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            VH+E D I+LNYTSGTT+ PKGVV SH+GA+LS LS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVV 300
           GWT TW +AARGGTNVC+R+ TA +I++NI LH VTHM C P VFR +L+    +     
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 WVVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKL 360
             V VLTGG+ PPA L++K+E LGFH+ H YGLTEATGP L CEWQ++WN LP  QQ +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNG 420
           + RQG+  LTLADVDVKN  T+ESVP DG T GEIV++GSSLMKGY K+P AT+ AFK+G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420

Query: 421 WFLTGDIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGDIGVI+PDGY+EIKDRSKD+IISGGENISS+E+E VLY + +V EAAVVAMPHP 
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480

Query: 481 WGESPCAFVAVHRDAEKI--SEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFK 540
           WGE+PCAFV + +  E +  SE ++I +CR+N+ H+M PKKVVF  ELPK S GKI K K
Sbjct: 481 WGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSK 540

Query: 541 LREQAKYFQVSEKRVASTSKNKSSRKDGSRR 565
           LR+ AK   V E    S   ++ S +  S R
Sbjct: 541 LRDIAKALVVREDDAGSKKVHQRSIEHVSSR 571

[Close]
BLAST of Lsi09G008870.1 vs. TAIR 10
Match: AT1G65880.1 (benzoyloxyglucosinolate 1 )

HSP 1 Score: 693.3 bits (1788), Expect = 1.6e-199
Identity = 346/561 (61.68%), Postives = 424/561 (75.58%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           MD L  C+AN   LTP+TFL RAS  Y +RTSIIY  TRFTW QTY+RCCRLA+SL  LN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           ISKNDVVSV+APN PALYEMHFAVPMAGAVLN INTRLDA +IA ILRH++ KILF+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YIKEAKDALRLLVAESFPIPL-VVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPV---- 180
           +   A+++L LL +E   + L V+FI + D P  A F  L+YE LI  G+   + V    
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180

Query: 181 EVHDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + DE D I+LNYTSGTT+ PKGVV SHRGA+L TLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVV 300
           GWTFTWG AARGGT+VCMR+ TA +I++NI +H+VTHMCC P VF I+L  +S +     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300

Query: 301 WVVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKL 360
             V+VLTGG+PPPA L++K++ LGF + HAYG TEATGP L CEWQ++WN LP +QQ +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNG 420
           KARQGISIL LADVDVKN +T +S P DG T GEI+++GSS+MKGY K+P AT  AFK+G
Sbjct: 361 KARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKHG 420

Query: 421 WFLTGDIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGD+GVI+PDG++EIKDRSKD+IISGGENISSVE+E VLY++P+V E AVVAMPHP 
Sbjct: 421 WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAVHR----------DAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTS 540
           WGE+PCAFV + +          D  +  E  +I +CR+NL H+M P+KVVF+ ELPK  
Sbjct: 481 WGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNG 540

Query: 541 TGKIQKFKLREQAKYFQVSEK 547
            GKI K KLR+ AK   V ++
Sbjct: 541 NGKILKPKLRDIAKGLVVEDE 561

[Close]
BLAST of Lsi09G008870.1 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 684.9 bits (1766), Expect = 5.7e-197
Identity = 345/569 (60.63%), Postives = 426/569 (74.87%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           MD L  C+AN   LTPITFL RAS  Y +RTSIIY  TRFTW QTY+RCCRLA+SL  LN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           I KNDVVSV+APN PA+YEMHFAVPMAGAVLN INTRLDA +IA ILRH++ KILF+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 YIKEAKDALRLLVAESFPIPL-VVFIDDIDSPTGARFGNLEYEQLIHDGDVKFTPVE--- 180
           +   A++ L+LL +E   + L V+FI +ID P        +YE LI  G+   TP+    
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEP--TPLLLAR 180

Query: 181 ---VHDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFH 240
              + DE D I+LNYTSGTT+ PKGVV SHRGA+LSTLS ++GWEMG  PVYLWTLPMFH
Sbjct: 181 MFCIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFH 240

Query: 241 CNGWTFTWGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRR 300
           CNGWTFTWG AARGGT+VCMR+ TA +I++NI +H+VTHMCC P VF I+L  +S +   
Sbjct: 241 CNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSH 300

Query: 301 VVWVVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQA 360
               V+VLTGG+PPPA L++K++ LGF + HAYGLTEATGP L CEWQ++WN LP +QQ 
Sbjct: 301 RSGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQM 360

Query: 361 KLKARQGISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFK 420
           +LKARQG+SIL L +VDV+N +T ESVP DG T GEIV++GSS+MKGY K+P AT  AFK
Sbjct: 361 ELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFK 420

Query: 421 NGWFLTGDIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPH 480
           +GW  +GD+GVI+PDG++EIKDRSKD+IISGGENISSVE+E ++Y++P+V E AVVAMPH
Sbjct: 421 HGWLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPH 480

Query: 481 PRWGESPCAFVAVHR---------DAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKT 540
           P WGE+PCAFV + +         D     E ++I +CR+NL H+M P+KVVF+ ELPK 
Sbjct: 481 PTWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKN 540

Query: 541 STGKIQKFKLREQAKYFQVSEKRVASTSK 554
             GKI K KLR+ AK   V+E  V   SK
Sbjct: 541 GNGKILKPKLRDIAKGL-VAEDEVNVRSK 566

[Close]
BLAST of Lsi09G008870.1 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 587.4 bits (1513), Expect = 1.2e-167
Identity = 284/543 (52.30%), Postives = 391/543 (72.01%), Query Frame = 0 

Query: 1   MDMLLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+  +K  ANY  LTPI+FL R++  YADR SI+Y   ++TWRQT +RC R+AS+L +L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQ 120
           IS  DVVSVLAPNVPA+ E+HF VPMAGA+L T+N R D++ +AV+LRHS  K++F D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YIKEAKDALRLLVAESFPIPLVVFIDDIDSPTGARFGN----LEYEQLIHDGDVKFTPVE 180
           +++ A+ A  +L  +   +P++V I +  + + +R       +EYE ++  G   F  + 
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 VHDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
             DE D+I++NYTSGTTS+PKGVVYSHRGA+L++L+ V+  EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFTWGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGNGRRVVW 300
           W   WG+ A GGTN+C+RN TA  IF NI+ H VTHM  AP +  +I++A     + +  
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 VVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQAKLK 360
            V+ +TG APPPA ++ KME LGF + H+YGLTE  GP  +C W+ +W+ LP ++QAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 ARQGISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAAAFKNGW 420
           ARQG++ L L ++ VK+  TM ++P DG T GE+V RG+++M GY K+P AT  AFK GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FLTGDIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRW 480
           F +GD+GV +PDGY+E+KDRSKD+IISGGENISS+E+E+ L+ HP V EAAVVA P   W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GESPCAFVAVHRDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLRE 540
           GE+ CAFV + +D  K S  E+I++CR  L HYM P+ +VF  +LPKTSTGK+QKF LR 
Sbjct: 483 GETACAFVKL-KDGSKASAEELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKFVLRT 542

[Close]
BLAST of Lsi09G008870.1 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 570.1 bits (1468), Expect = 2.0e-162
Identity = 285/548 (52.01%), Postives = 380/548 (69.34%), Query Frame = 0 

Query: 4   LLKCDANYASLTPITFLTRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLCRLNISK 63
           LL+  AN++ L+PITFL R++  Y DRTS+++   + TW QTY+RC RLAS+L  L IS+
Sbjct: 51  LLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLGISR 110

Query: 64  NDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDANNIAVILRHSEAKILFVDYQYIK 123
            DVV+ LAPNVPA++E+HFAVPMAG +L  +NTRLD + ++V+L HSEAKILFVD+Q ++
Sbjct: 111 GDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLE 170

Query: 124 EAKDALRLL-----VAESFPIPLVVFI---DDIDSPTGARFG-----NLEYEQLIHDGDV 183
            A  AL LL       +S  + L+      DD D  + + F      + EYE L+  GD 
Sbjct: 171 IAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDS 230

Query: 184 KFTPVEVHDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLP 243
           +F  ++   EWD I++NYTSGTTS PKGVVYSHRGA+L++L+ V   +M   PVYLWT+P
Sbjct: 231 EFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLWTVP 290

Query: 244 MFHCNGWTFTWGIAARGGTNVCMRNTTALDIFRNINLHHVTHMCCAPIVFRIILDADSGN 303
           MFHCNGW   WG+AA+GGTN+C+R  +   IF+NI +H VTHM  AP V  +I++     
Sbjct: 291 MFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTE 350

Query: 304 GRRVVWVVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPAD 363
            + +   V ++TGG+PP   +L KME LGF+++H YGLTE  GP   C W+ +W+ L  +
Sbjct: 351 HKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLE 410

Query: 364 QQAKLKARQGISILTLADVDVKNLDTMESVPHDGHTSGEIVLRGSSLMKGYFKDPAATAA 423
           ++ KLKARQG+  L L  +DVK+  TME+VP DG T GE++ RG+++M GYFKD  AT  
Sbjct: 411 ERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRK 470

Query: 424 AFKNGWFLTGDIGVIYPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVA 483
           AF+  WF +GD+ V YPDGY+EIKDR KDVIISGGENISSVE+E VL  H  V EAAVVA
Sbjct: 471 AFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAAVVA 530

Query: 484 MPHPRWGESPCAFVAVHRDAEKISEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQ 539
            P   WG++PC FV +    + I   EII FCR +L HYM PK +VF  ++PKTSTGK+Q
Sbjct: 531 RPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVF-GDIPKTSTGKVQ 590

The following BLAST results are available for this feature:
BLAST of Lsi09G008870.1 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
I3PB362.0e-21566.25Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 S... [more]
Q9C8D42.0e-19961.12Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11... [more]
Q9SS012.3e-19861.68Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 ... [more]
Q9SS008.0e-19660.63Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 ... [more]
F4HUK61.7e-16652.30Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1[more]
BLAST of Lsi09G008870.1 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1ECD91.9e-29686.83butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A0A0KT401.2e-29587.69Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 G... [more]
A0A5A7TXW01.7e-29487.76Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3CIM11.7e-29487.76butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A6J1JUR42.5e-29385.96butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
BLAST of Lsi09G008870.1 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038906606.13.4e-30890.64trans-cinnamate:CoA ligase, peroxisomal-like [Benincasa hispida][more]
XP_038881152.18.8e-30188.91LOW QUALITY PROTEIN: trans-cinnamate:CoA ligase, peroxisomal-like [Benincasa his... [more]
XP_011655134.13.8e-29687.56butyrate--CoA ligase AAE11, peroxisomal [Cucumis sativus] >KGN51049.2 hypothetic... [more]
XP_022923630.13.8e-29686.83butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata][more]
KAG6602004.15.6e-29586.66Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sorori... [more]
BLAST of Lsi09G008870.1 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G66120.11.4e-20061.12AMP-dependent synthetase and ligase family protein [more]
AT1G65880.11.6e-19961.68benzoyloxyglucosinolate 1 [more]
AT1G65890.15.7e-19760.63acyl activating enzyme 12 [more]
AT1G20560.11.2e-16752.30acyl activating enzyme 1 [more]
AT2G17650.12.0e-16252.01AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 4..438
e-value: 1.4E-98
score: 332.2
NoneNo IPR availableGENE3D3.30.300.30coord: 439..541
e-value: 1.2E-33
score: 117.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 547..577
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 551..571
NoneNo IPR availablePANTHERPTHR43859:SF2BUTYRATE--COA LIGASE AAE11, PEROXISOMALcoord: 1..555
NoneNo IPR availablePANTHERPTHR43859ACYL-ACTIVATING ENZYMEcoord: 1..555
NoneNo IPR availableCDDcd12118ttLC_FACS_AEE21_likecoord: 11..536
e-value: 0.0
score: 833.101
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 13..539
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 452..528
e-value: 1.1E-24
score: 87.1
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 23..443
e-value: 4.6E-94
score: 315.4
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 186..197

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi09G008870Lsi09G008870gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi09G008870.1.exon.3Lsi09G008870.1.exon.3exon
Lsi09G008870.1.exon.2Lsi09G008870.1.exon.2exon
Lsi09G008870.1.exon.1Lsi09G008870.1.exon.1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi09G008870.1.CDS.3Lsi09G008870.1.CDS.3CDS
Lsi09G008870.1.CDS.2Lsi09G008870.1.CDS.2CDS
Lsi09G008870.1.CDS.1Lsi09G008870.1.CDS.1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi09G008870.1.five_prime_UTR.1Lsi09G008870.1.five_prime_UTR.1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi09G008870.1Lsi09G008870.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016874 ligase activity