Lsi09G003660.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G003660.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein DETOXIFICATION
Locationchr09: 3799674 .. 3801137 (+)
Sequence length1464
RNA-Seq ExpressionLsi09G003660.1
SyntenyLsi09G003660.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGTATCTGCATCTTCTTCAACCTTGGAATCTGGATCAAGGCCCAGAATTACGAAATGGGTTTCAAAAGATTTGACTAACTCCATTCTTTCTGAGCTGAAATTGCAGAGAGGCCTTGCTCTTCCACTCATCGCTATGAACTTAACATGGTTTGTAAAGATAGCCATAACCACCGCTTTCCTTGGCCGTCTCGGCGAGCTTCCATTAGCCGGTGGAACACTTGGTTTCACCTTCGCCAATGTCACTGGCTTTTCCGTTTTGAACGGTCTCTGTGGCGCCATGGAACCCATCTGCGGCCAAGCCTTCGGCGCCAAGAATTTCAAACTCCTCCACAAAACTCTTCTCATGTCTATCTTTCTTTTACTCCTCGCTACACTCCCGATTTCATTCCTCTGGCTCAACGTGGACAAAATTTTGATCAAATTCGGTCAACAGAAGGACATTTCACTCGCCGCTAAAACCTATCTCTTCTACCTTCTCCCTGATTTGGTTATCACTTCATTTCTCTGCCCATTGAAATCCTATCTCAGTTCACAAACCGAAACGCTTCCGATCATGTTAACCTCTGCTCTGGCTCTGGCTCTTCATGTACCCATCAACATTTTCCTTGCTAAATCCAAGGGCTTAATTGGGGTTTCAATAGCGATTTGGGTAACTGATTTTGTGGCAATGATTTCACTAGCGATTTACGTTTGGGTGAAACAGAGGATGATGAGTAATAATGGAGAAAAAGGTGGATGGTTTGATCAAACGGTTCAGGATTGGGTTCGTTTAGTAAAGCTTTCAGGTCCGTGTTGCTTAACCACCTGCCTTGAATGGTGGTGTTACGAGATTCTGATTCTTCTTACTGGCCGTCTTCCCAATGCCAAACAAGCCGTGGGGACCATAGCCATAGTATTAAACTTCGATTATTTGCTTTATTCTGTTATGTTATCGTTAGCCACGTGTGCGTCGGCGCGTGTGTCGAACGAGCTGGGTGGAAACCGTGGAGTGGAGGCGAGGTGGTCGGCGGGGGTGTCGGTGGTGGGGAGTGTTGTGTTTGGGTTGATGGGGGCGGCGGCGATGGTGGCGGGGAGAGGGGAATGGGGGAGGATTTTCACTAAAGATGAAGGGGTAGTGAGAATGGTGAGGAAGATGTTGGTTTTGATGGCGGCGATTGAGGTAGTGAATTTTCCGGTGGCCGTTTGCGGCGGAGTAGTGAGAGGAGCAGGGAAGCCGTTGATGGGATTGTGTGCGAGTCTTGGTGGGTTCTACGGAGTGGCTTTGCCTTTGGGTGTGGTGTTGGGGTTTAAAGTTGGGGTTGGGCTTGGTGGGCTGTTGATTGGGTTCTTGGTTGGGATGTTTGGTTGTTTGGTTTTGTTAATGGTGTTTGTTGGGAGGATTGATTGGGGAAAGGAAGCTCAAAGGGCTCAACTTATGGCTAAAGATGGAGAGATTGTTGTGGATGATGTAAAAATATGA

mRNA sequence

ATGTCTGTATCTGCATCTTCTTCAACCTTGGAATCTGGATCAAGGCCCAGAATTACGAAATGGGTTTCAAAAGATTTGACTAACTCCATTCTTTCTGAGCTGAAATTGCAGAGAGGCCTTGCTCTTCCACTCATCGCTATGAACTTAACATGGTTTGTAAAGATAGCCATAACCACCGCTTTCCTTGGCCGTCTCGGCGAGCTTCCATTAGCCGGTGGAACACTTGGTTTCACCTTCGCCAATGTCACTGGCTTTTCCGTTTTGAACGGTCTCTGTGGCGCCATGGAACCCATCTGCGGCCAAGCCTTCGGCGCCAAGAATTTCAAACTCCTCCACAAAACTCTTCTCATGTCTATCTTTCTTTTACTCCTCGCTACACTCCCGATTTCATTCCTCTGGCTCAACGTGGACAAAATTTTGATCAAATTCGGTCAACAGAAGGACATTTCACTCGCCGCTAAAACCTATCTCTTCTACCTTCTCCCTGATTTGGTTATCACTTCATTTCTCTGCCCATTGAAATCCTATCTCAGTTCACAAACCGAAACGCTTCCGATCATGTTAACCTCTGCTCTGGCTCTGGCTCTTCATGTACCCATCAACATTTTCCTTGCTAAATCCAAGGGCTTAATTGGGGTTTCAATAGCGATTTGGGTAACTGATTTTGTGGCAATGATTTCACTAGCGATTTACGTTTGGGTGAAACAGAGGATGATGAGTAATAATGGAGAAAAAGGTGGATGGTTTGATCAAACGGTTCAGGATTGGGTTCGTTTAGTAAAGCTTTCAGGTCCGTGTTGCTTAACCACCTGCCTTGAATGGTGGTGTTACGAGATTCTGATTCTTCTTACTGGCCGTCTTCCCAATGCCAAACAAGCCGTGGGGACCATAGCCATAGTATTAAACTTCGATTATTTGCTTTATTCTGTTATGTTATCGTTAGCCACGTGTGCGTCGGCGCGTGTGTCGAACGAGCTGGGTGGAAACCGTGGAGTGGAGGCGAGGTGGTCGGCGGGGGTGTCGGTGGTGGGGAGTGTTGTGTTTGGGTTGATGGGGGCGGCGGCGATGGTGGCGGGGAGAGGGGAATGGGGGAGGATTTTCACTAAAGATGAAGGGGTAGTGAGAATGGTGAGGAAGATGTTGGTTTTGATGGCGGCGATTGAGGTAGTGAATTTTCCGGTGGCCGTTTGCGGCGGAGTAGTGAGAGGAGCAGGGAAGCCGTTGATGGGATTGTGTGCGAGTCTTGGTGGGTTCTACGGAGTGGCTTTGCCTTTGGGTGTGGTGTTGGGGTTTAAAGTTGGGGTTGGGCTTGGTGGGCTGTTGATTGGGTTCTTGGTTGGGATGTTTGGTTGTTTGGTTTTGTTAATGGTGTTTGTTGGGAGGATTGATTGGGGAAAGGAAGCTCAAAGGGCTCAACTTATGGCTAAAGATGGAGAGATTGTTGTGGATGATGTAAAAATATGA

Coding sequence (CDS)

ATGTCTGTATCTGCATCTTCTTCAACCTTGGAATCTGGATCAAGGCCCAGAATTACGAAATGGGTTTCAAAAGATTTGACTAACTCCATTCTTTCTGAGCTGAAATTGCAGAGAGGCCTTGCTCTTCCACTCATCGCTATGAACTTAACATGGTTTGTAAAGATAGCCATAACCACCGCTTTCCTTGGCCGTCTCGGCGAGCTTCCATTAGCCGGTGGAACACTTGGTTTCACCTTCGCCAATGTCACTGGCTTTTCCGTTTTGAACGGTCTCTGTGGCGCCATGGAACCCATCTGCGGCCAAGCCTTCGGCGCCAAGAATTTCAAACTCCTCCACAAAACTCTTCTCATGTCTATCTTTCTTTTACTCCTCGCTACACTCCCGATTTCATTCCTCTGGCTCAACGTGGACAAAATTTTGATCAAATTCGGTCAACAGAAGGACATTTCACTCGCCGCTAAAACCTATCTCTTCTACCTTCTCCCTGATTTGGTTATCACTTCATTTCTCTGCCCATTGAAATCCTATCTCAGTTCACAAACCGAAACGCTTCCGATCATGTTAACCTCTGCTCTGGCTCTGGCTCTTCATGTACCCATCAACATTTTCCTTGCTAAATCCAAGGGCTTAATTGGGGTTTCAATAGCGATTTGGGTAACTGATTTTGTGGCAATGATTTCACTAGCGATTTACGTTTGGGTGAAACAGAGGATGATGAGTAATAATGGAGAAAAAGGTGGATGGTTTGATCAAACGGTTCAGGATTGGGTTCGTTTAGTAAAGCTTTCAGGTCCGTGTTGCTTAACCACCTGCCTTGAATGGTGGTGTTACGAGATTCTGATTCTTCTTACTGGCCGTCTTCCCAATGCCAAACAAGCCGTGGGGACCATAGCCATAGTATTAAACTTCGATTATTTGCTTTATTCTGTTATGTTATCGTTAGCCACGTGTGCGTCGGCGCGTGTGTCGAACGAGCTGGGTGGAAACCGTGGAGTGGAGGCGAGGTGGTCGGCGGGGGTGTCGGTGGTGGGGAGTGTTGTGTTTGGGTTGATGGGGGCGGCGGCGATGGTGGCGGGGAGAGGGGAATGGGGGAGGATTTTCACTAAAGATGAAGGGGTAGTGAGAATGGTGAGGAAGATGTTGGTTTTGATGGCGGCGATTGAGGTAGTGAATTTTCCGGTGGCCGTTTGCGGCGGAGTAGTGAGAGGAGCAGGGAAGCCGTTGATGGGATTGTGTGCGAGTCTTGGTGGGTTCTACGGAGTGGCTTTGCCTTTGGGTGTGGTGTTGGGGTTTAAAGTTGGGGTTGGGCTTGGTGGGCTGTTGATTGGGTTCTTGGTTGGGATGTTTGGTTGTTTGGTTTTGTTAATGGTGTTTGTTGGGAGGATTGATTGGGGAAAGGAAGCTCAAAGGGCTCAACTTATGGCTAAAGATGGAGAGATTGTTGTGGATGATGTAAAAATATGA

Protein sequence

MSVSASSSTLESGSRPRITKWVSKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITTAFLGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQTETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMSNNGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLNFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGRGEWGRIFTKDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYGVALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDGEIVVDDVKI
Homology
BLAST of Lsi09G003660.1 vs. ExPASy Swiss-Prot
Match: O49660 (Protein DETOXIFICATION 56 OS=Arabidopsis thaliana OX=3702 GN=DTX56 PE=1 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 2.5e-150
Identity = 280/481 (58.21%), Postives = 360/481 (74.84%), Query Frame = 0

Query: 1   MSVSASSSTLESGSRPRITKWV-SKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITT 60
           MS ++ S +L+    P +++ + SK L  SI+ ELKLQ  + LPL+ MNL WF K+  T+
Sbjct: 1   MSETSKSESLD----PEVSEGLCSKTLMQSIVHELKLQMRIGLPLVVMNLLWFGKMTTTS 60

Query: 61  AFLGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSI 120
            FLGR GEL LAGG+LGF+FANVTGFSVL G+  AMEPICGQAFGAKNFKLLHKTL M++
Sbjct: 61  VFLGRQGELNLAGGSLGFSFANVTGFSVLYGISAAMEPICGQAFGAKNFKLLHKTLFMAV 120

Query: 121 FLLLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSS 180
            LLLL ++PISFLWLNV KIL  FGQ++DIS  AK YL YLLP+L I SFLCPLK+YLSS
Sbjct: 121 LLLLLISVPISFLWLNVHKILTGFGQREDISFIAKKYLLYLLPELPILSFLCPLKAYLSS 180

Query: 181 QTETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMM 240
           Q  TLPIM T+A A +LH+PINI L+K++G+ GV++A+W+TDF+ +I L  YV V +RM 
Sbjct: 181 QGVTLPIMFTTAAATSLHIPINIVLSKARGIEGVAMAVWITDFIVVILLTGYVIVVERMK 240

Query: 241 SNNGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAI 300
            N  ++GGW +Q+ QDW+ L+KLSGPCCLT CLEWWCYEIL+LLTGRLPN  QAV  + I
Sbjct: 241 ENKWKQGGWLNQSAQDWLTLIKLSGPCCLTVCLEWWCYEILVLLTGRLPNPVQAVSILII 300

Query: 301 VLNFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAG 360
           V NFDYLLY+VMLSL TC + RVSNELG N    A  +A  +++  ++ G +GA  M+A 
Sbjct: 301 VFNFDYLLYAVMLSLGTCVATRVSNELGANNPKGAYRAAYTTLIVGIISGCIGALVMIAF 360

Query: 361 RGEWGRIFT-KDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGF 420
           RG WG ++T  D+ ++  V+KM+++MA IEVVNFP+ VCG +VRG  KP +G+ A+L GF
Sbjct: 361 RGFWGSLYTHHDQLILNGVKKMMLIMAVIEVVNFPLMVCGEIVRGTAKPSLGMYANLSGF 420

Query: 421 YGVALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDG 480
           Y +ALPLG  L FK   GL G LIG  VG+  CL +L++F+ RIDW KEA +AQ++  + 
Sbjct: 421 YLLALPLGATLAFKAKQGLQGFLIGLFVGISLCLSILLIFIARIDWEKEAGKAQILTCNT 477

BLAST of Lsi09G003660.1 vs. ExPASy Swiss-Prot
Match: Q9SZE2 (Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 8.0e-80
Identity = 180/460 (39.13%), Postives = 260/460 (56.52%), Query Frame = 0

Query: 31  LSELKLQRGLALPLIAMNLTWFVKIAITTAFLGRLGELPLAGGTLGFTFANVTGFSVLNG 90
           ++E K    LA P+    L  +++ A++  FLG+LG+L LA G+L   FAN+TG+SVL+G
Sbjct: 54  VTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVLSG 113

Query: 91  LCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLATLPISFLWLNVDKILIKFGQQKDIS 150
           L   MEP+C QAFGA  FKLL  TL  ++  LL+  +PIS LW NV KI +   Q  DI+
Sbjct: 114 LALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPDIA 173

Query: 151 LAAKTYLFYLLPDLVITSFLCPLKSYLSSQTETLPIMLTSALALALHVPINIFLAK--SK 210
             A+TYL + LPDL+  + L P++ YL +Q    P+ L S      H+P N+FL      
Sbjct: 174 KLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYLRL 233

Query: 211 GLIGVSIAIWVTDFVAMISLAIYVWVKQRMMSNNGEKGGWFDQT---VQDWVRLVKLSGP 270
           GL GV++A  +T+   +  L  YVW      ++      W D T    + W  L++L+GP
Sbjct: 234 GLTGVAVASSITNIFVVAFLVCYVW------ASGLHAPTWTDPTRDCFRGWAPLLRLAGP 293

Query: 271 CCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLNFDYLLYSVMLSLATCASARVSNE 330
            C++ CLEWW YEI+I+L G L N +  V  + +++     LY    SL+   S RV NE
Sbjct: 294 SCVSVCLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNE 353

Query: 331 LGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGRGEWGRIFTKDEGVVRMVRKMLVLMA 390
           LG NR   A+ +A V++V + V G++ AA   + R  WGRIFT D+ ++++    L ++ 
Sbjct: 354 LGANRPKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILG 413

Query: 391 AIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYGVALPLGVVLGFKVGVGLGGLLIGFL 450
             E+ N P  V  GVVRG  +P      +LG FY V +P+ V LGF  G+G  GL +G L
Sbjct: 414 LCEIGNCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLL 473

Query: 451 VGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDGEIVVDDV 486
                C  L+M  VG  DW  EA++AQ +    E V +D+
Sbjct: 474 AAQISCAGLMMYVVGTTDWESEAKKAQTLT-CAETVENDI 506

BLAST of Lsi09G003660.1 vs. ExPASy Swiss-Prot
Match: Q9LE20 (Protein DETOXIFICATION 54 OS=Arabidopsis thaliana OX=3702 GN=DTX54 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 5.4e-76
Identity = 177/459 (38.56%), Postives = 264/459 (57.52%), Query Frame = 0

Query: 30  ILSELKLQRGLALPLIAMNLTWFVKIAITTAFLGRLGELPLAGGTLGFTFANVTGFSVLN 89
           ++ ELK    + LP+ AMN   +V+  ++  FLGRLG L LAGG L   F N+TG+SV+ 
Sbjct: 21  VIEELKELWAMVLPITAMNCLVYVRAVVSVLFLGRLGSLELAGGALSIGFTNITGYSVMV 80

Query: 90  GLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLATLPISFLWLNVDKILIKFGQQKDI 149
           GL   +EP+C QA+G+KN+ LL  +L   + +LL+A+LPIS LW+N+  I++  GQ  +I
Sbjct: 81  GLASGLEPVCSQAYGSKNWDLLTLSLHRMVVILLMASLPISLLWINLGPIMLFMGQNPEI 140

Query: 150 SLAAKTYLFYLLPDLVITSFLCPLKSYLSSQTETLPIMLTSALALALHVPINIFLAKSK- 209
           +  A  Y  Y LPDL+  + L PL+ YL SQ  T P+M  +  A+A HVP+N +L   K 
Sbjct: 141 TATAAEYCLYALPDLLTNTLLQPLRVYLRSQRVTKPMMWCTLAAVAFHVPLNYWLVMVKH 200

Query: 210 -GLIGVSIAIWVTDFVAMISLAIYVWVK---QRMMSNNGEKGGWF-------DQTVQDWV 269
            G+ GV+IA  VT+ + ++ L  YVWV    Q+ +S +G+ G            +V + V
Sbjct: 201 WGVPGVAIASVVTNLIMVVLLVGYVWVSGMLQKRVSGDGDGGSTTMVAVVAQSSSVMELV 260

Query: 270 ----RLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLNFDYLLYSVMLS 329
                L++++ P CL  CLEWW YEI+I++ G L N K AV    I++    L+Y+V ++
Sbjct: 261 GGLGPLMRVAVPSCLGICLEWWWYEIVIVMGGYLENPKLAVAATGILIQTTSLMYTVPMA 320

Query: 330 LATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGRGEWGRIFTKDEGV 389
           LA C SARV NELG  R  +AR +A V++  + V G +  A  V  +  W  +FT  E +
Sbjct: 321 LAGCVSARVGNELGAGRPYKARLAANVALACAFVVGALNVAWTVILKERWAGLFTGYEPL 380

Query: 390 VRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYGVALPLGVVLGFKV 449
             +V  ++ ++   E+ N P     G++RG G+P +G   +LG FY V  P+ V L F +
Sbjct: 381 KVLVASVMPIVGLCELGNCPQTTGCGILRGTGRPAVGAHVNLGSFYFVGTPVAVGLAFWL 440

Query: 450 GVGLGGLLIGFLVGMFGCLV-LLMVFVGRIDWGKEAQRA 472
            +G  GL  G L     C+V +L   + R DW  EA +A
Sbjct: 441 KIGFSGLWFGLLSAQAACVVSILYAVLARTDWEGEAVKA 479

BLAST of Lsi09G003660.1 vs. ExPASy Swiss-Prot
Match: Q9FH21 (Protein DETOXIFICATION 55 OS=Arabidopsis thaliana OX=3702 GN=DTX55 PE=2 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.2e-75
Identity = 169/455 (37.14%), Postives = 257/455 (56.48%), Query Frame = 0

Query: 30  ILSELKLQRGLALPLIAMNLTWFVKIAITTAFLGRLGELPLAGGTLGFTFANVTGFSVLN 89
           ++ ELK    ++ P+ AM++  ++K   +   +GRLG L LAGG L   F N+TG+SVL+
Sbjct: 23  VVEELKRIWDISFPVAAMSILNYLKNMTSVVCMGRLGSLELAGGALAIGFTNITGYSVLS 82

Query: 90  GLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLATLPISFLWLNVDKILIKFGQQKDI 149
           GL   MEP+CGQA G+KN  L   TL  +IFLLLLA+LPIS LWLN+  +++   QQ DI
Sbjct: 83  GLATGMEPLCGQAIGSKNPSLASLTLKRTIFLLLLASLPISLLWLNLAPLMLMLRQQHDI 142

Query: 150 SLAAKTYLFYLLPDLVITSFLCPLKSYLSSQTETLPIMLTSALALALHVPINIFLA--KS 209
           +  A  Y  + LPDL+  SFL PL+ YL  +  T P+M  + +++ LH+PI  F     S
Sbjct: 143 TRVASLYCSFSLPDLLANSFLHPLRIYLRCKGTTWPLMWCTLVSVLLHLPITAFFTFYIS 202

Query: 210 KGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMSNNGEKG----------GWFDQTVQD-W 269
            G+ GV+++ ++T+F+++  L  Y++++         K           G  D    D W
Sbjct: 203 LGVPGVAVSSFLTNFISLSLLLCYIYLENNNNDKTTSKSLCLDTPLMLYGSRDSGENDVW 262

Query: 270 VRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLNFDYLLYSVMLSLAT 329
             LVK + P C+  CLEWW YE + +L G LP  K A+   AIV+    L+Y++  +L+ 
Sbjct: 263 STLVKFAVPSCIAVCLEWWWYEFMTVLAGYLPEPKVALAAAAIVIQTTSLMYTIPTALSA 322

Query: 330 CASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGRGEWGRIFTKDEGVVRM 389
             S RVSNELG  R  +A+ +A V+V  +V   + G      GR  WG++FT D+ V+ +
Sbjct: 323 AVSTRVSNELGAGRPEKAKTAATVAVGAAVAVSVFGLVGTTVGREAWGKVFTADKVVLEL 382

Query: 390 VRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYGVALPLGVVLGFKVGVG 449
              ++ ++ A E+ N P  +  G++RG+ +P +G   +   FY V  P+ VVL F  G+G
Sbjct: 383 TAAVIPVIGACELANCPQTISCGILRGSARPGIGAKINFYAFYVVGAPVAVVLAFVWGLG 442

Query: 450 LGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRA 472
             GL  G L     C + ++  V   DW KE+ +A
Sbjct: 443 FMGLCYGLLGAQLACAISILTVVYNTDWNKESLKA 477

BLAST of Lsi09G003660.1 vs. ExPASy Swiss-Prot
Match: O82752 (Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.1e-73
Identity = 185/464 (39.87%), Postives = 271/464 (58.41%), Query Frame = 0

Query: 28  NSILSELKLQRGLALPLIAMNLTWFVKIAITTAFLGRLGEL-PLAGGTLGFTFANVTGFS 87
           +S + E K    ++LPLI   L  + +  I+  FLGRL +L  L+GG+L   FAN+TG+S
Sbjct: 27  SSSIQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGYS 86

Query: 88  VLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLATLPISFLWLNVDKILIKFGQQ 147
           +L+GL   MEPIC QAFGAK FKLL   L  +  LLLL +LPIS LWLN+ KIL+ FGQ 
Sbjct: 87  LLSGLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQD 146

Query: 148 KDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQTETLPIMLTSALALALHVPINIFLAK 207
           ++IS  A+ ++ + LPDL++ SFL P++ YL SQ+ TLP+  ++  A+ LH+PIN  L  
Sbjct: 147 EEISNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLVS 206

Query: 208 S--KGLIGVSI-AIWVTDFVAMISLAIYVWVKQRMMSNNGEK--GGWFDQTVQDWVRLVK 267
           S   GL GV++ AIW T+   +  L IY+     + S   +K  GG+     + W  L+K
Sbjct: 207 SLGLGLKGVALGAIW-TNVNLLGFLIIYI-----VFSGVYQKTWGGFSMDCFKGWRSLMK 266

Query: 268 LSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLNFDYLLYSVMLSLATCASAR 327
           L+ P C++ CLEWW YEI+ILL G L N +  V ++ I++    L+Y    SL+   S R
Sbjct: 267 LAIPSCVSVCLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTR 326

Query: 328 VSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGRGEWGRIFTKDEGVVRMVRKML 387
           V NELG N+  +AR +A   +  S+  GL+     +  R  W R+FT +E +V++   +L
Sbjct: 327 VGNELGANQPDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVL 386

Query: 388 VLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYGVALPLGVVLGFKVGVGLGGLL 447
            ++   E+ N P     GV+RG+ +P +G   +L  FY V +P+ V L F  G    GL 
Sbjct: 387 PIIGLCELGNCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLW 446

Query: 448 IGFLVGMFGCLVLLMVFVGRIDWGKEAQRA-QLMAKDGEIVVDD 485
           +G       CL+ ++V + R DW  E  RA +LM +  +   DD
Sbjct: 447 LGLFAAQGSCLISMLVVLARTDWEVEVHRAKELMTRSCDGDEDD 484

BLAST of Lsi09G003660.1 vs. ExPASy TrEMBL
Match: A0A1S3ATW5 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103482671 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.1e-240
Identity = 448/487 (91.99%), Postives = 462/487 (94.87%), Query Frame = 0

Query: 1   MSVSASSSTLESGSRPRITKWVSKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITTA 60
           MSV+ SSSTLESGS PRITKWVSKD  NSILSELKLQRG+ALPLIAMNLTWFVKIAITTA
Sbjct: 1   MSVTVSSSTLESGSSPRITKWVSKDFINSILSELKLQRGIALPLIAMNLTWFVKIAITTA 60

Query: 61  FLGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIF 120
           FLGRLG+LPLA GTLGFTFANVTGFSVLNGLC AMEPICGQAFGAKNFKLLHKTL MSIF
Sbjct: 61  FLGRLGDLPLAAGTLGFTFANVTGFSVLNGLCCAMEPICGQAFGAKNFKLLHKTLFMSIF 120

Query: 121 LLLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQ 180
           LLLLATLPISFLWLNVD ILI FGQQKDIS+AAKTYLFYLLPDL+ITSFLCPLKSYLSSQ
Sbjct: 121 LLLLATLPISFLWLNVDTILIHFGQQKDISIAAKTYLFYLLPDLLITSFLCPLKSYLSSQ 180

Query: 181 TETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMS 240
           TETLP ML+SALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVW+KQ  MS
Sbjct: 181 TETLPTMLSSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWLKQ-SMS 240

Query: 241 NNGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300
           NN E GGWFDQTVQDWVRL+KLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV
Sbjct: 241 NNEEGGGWFDQTVQDWVRLLKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300

Query: 301 LNFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGR 360
           LNFDYLLYSVMLSLATCASARVSNELG N G+ ARWSAGVSVVGSVV GLMGAAAMVAGR
Sbjct: 301 LNFDYLLYSVMLSLATCASARVSNELGRNSGLAARWSAGVSVVGSVVVGLMGAAAMVAGR 360

Query: 361 GEWGRIFTKDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYG 420
           GEWGRIFT+DE VVRMV+KMLVLMAAIEVVNFPVAVCGGVVRG GKPLMGL ASLGGFYG
Sbjct: 361 GEWGRIFTRDEEVVRMVKKMLVLMAAIEVVNFPVAVCGGVVRGVGKPLMGLGASLGGFYG 420

Query: 421 VALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDGE- 480
           VALPLGVVLGFKVGVGLGGLLIGFLVG+F CLVLLMVFV RIDWGKEAQ AQLMAKDGE 
Sbjct: 421 VALPLGVVLGFKVGVGLGGLLIGFLVGVFVCLVLLMVFVWRIDWGKEAQAAQLMAKDGEV 480

Query: 481 IVVDDVK 487
           +VVD+VK
Sbjct: 481 VVVDNVK 486

BLAST of Lsi09G003660.1 vs. ExPASy TrEMBL
Match: A0A5A7TN93 (Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003480 PE=3 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.6e-240
Identity = 447/487 (91.79%), Postives = 462/487 (94.87%), Query Frame = 0

Query: 1   MSVSASSSTLESGSRPRITKWVSKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITTA 60
           MSV+ SSSTLESGS PRITKWVSKD  NSILSELKLQRG+ALPLIAMNLTWFVKIAITTA
Sbjct: 1   MSVTVSSSTLESGSSPRITKWVSKDFINSILSELKLQRGIALPLIAMNLTWFVKIAITTA 60

Query: 61  FLGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIF 120
           FLGRLG+LPLA GTLGFTFANVTGFSVLNGLC AMEPICGQAFGAKNFKLLHKTL MSIF
Sbjct: 61  FLGRLGDLPLAAGTLGFTFANVTGFSVLNGLCCAMEPICGQAFGAKNFKLLHKTLFMSIF 120

Query: 121 LLLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQ 180
           LLLLATLPISFLWLNVD ILI FGQQKDIS+AAKTYLFYLLPDL+ITSFLCPLKSYLSSQ
Sbjct: 121 LLLLATLPISFLWLNVDTILIHFGQQKDISIAAKTYLFYLLPDLLITSFLCPLKSYLSSQ 180

Query: 181 TETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMS 240
           TETLP ML+SALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVW+KQ  MS
Sbjct: 181 TETLPTMLSSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWLKQ-SMS 240

Query: 241 NNGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300
           NN E GGWFDQTVQDWVRL+KLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV
Sbjct: 241 NNEEGGGWFDQTVQDWVRLLKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300

Query: 301 LNFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGR 360
           LNFDYLLYSVMLSLATCASARVSNELG N G+ ARWSAGVSVVGSVV GLMGAAAMVAGR
Sbjct: 301 LNFDYLLYSVMLSLATCASARVSNELGRNSGLAARWSAGVSVVGSVVVGLMGAAAMVAGR 360

Query: 361 GEWGRIFTKDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYG 420
           GEWGRIFT+DE VVRMV+KMLVLMAAIEVVNFPVAVCGGVVRG GKPLMGL ASLGGFYG
Sbjct: 361 GEWGRIFTRDEEVVRMVKKMLVLMAAIEVVNFPVAVCGGVVRGVGKPLMGLGASLGGFYG 420

Query: 421 VALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDGE- 480
           VALPLG+VLGFKVGVGLGGLLIGFLVG+F CLVLLMVFV RIDWGKEAQ AQLMAKDGE 
Sbjct: 421 VALPLGMVLGFKVGVGLGGLLIGFLVGVFVCLVLLMVFVWRIDWGKEAQAAQLMAKDGEV 480

Query: 481 IVVDDVK 487
           +VVD+VK
Sbjct: 481 VVVDNVK 486

BLAST of Lsi09G003660.1 vs. ExPASy TrEMBL
Match: A0A0A0KMR7 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G157920 PE=3 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.6e-240
Identity = 444/487 (91.17%), Postives = 464/487 (95.28%), Query Frame = 0

Query: 1   MSVSASSSTLESGSRPRITKWVSKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITTA 60
           MSV+ SSSTLESGS PRITKWVSKD  NSI+SELKLQRG+ALPL+AMNLTWFVKIAITTA
Sbjct: 1   MSVTVSSSTLESGSSPRITKWVSKDFINSIISELKLQRGIALPLVAMNLTWFVKIAITTA 60

Query: 61  FLGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIF 120
           FLGRLG+LPLA GTLGFTFANVTGFSVLNGLC AMEPICGQAFGAKNF+LLHKTL MSIF
Sbjct: 61  FLGRLGDLPLAAGTLGFTFANVTGFSVLNGLCCAMEPICGQAFGAKNFQLLHKTLFMSIF 120

Query: 121 LLLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQ 180
           LLLLATLPISFLWLNVD ILI FGQQKD+S+AAKTYLFYLLPDL+ITSFLCPLKSYLSSQ
Sbjct: 121 LLLLATLPISFLWLNVDTILIHFGQQKDLSIAAKTYLFYLLPDLLITSFLCPLKSYLSSQ 180

Query: 181 TETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMS 240
           TETLPIML+SALALALHVPIN+FLAKSKGLIGVSIAIWVTDFVAMISLAIYVW+KQ  MS
Sbjct: 181 TETLPIMLSSALALALHVPINLFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWLKQ-SMS 240

Query: 241 NNGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300
           NN E GGWFDQTVQDWVRL+KLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV
Sbjct: 241 NNEEGGGWFDQTVQDWVRLLKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300

Query: 301 LNFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGR 360
           LNFDYLLYSVMLSLATCASARVSNELG N  + ARWSAGVSVVGSVV GLMGAAAMVAGR
Sbjct: 301 LNFDYLLYSVMLSLATCASARVSNELGRNSELAARWSAGVSVVGSVVVGLMGAAAMVAGR 360

Query: 361 GEWGRIFTKDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYG 420
           GEWGRIFT+DEGVVRMV+KMLVLMAAIEVVNFPVAVCGGVVRG GKPLMGL ASLGGFYG
Sbjct: 361 GEWGRIFTRDEGVVRMVKKMLVLMAAIEVVNFPVAVCGGVVRGVGKPLMGLGASLGGFYG 420

Query: 421 VALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDGEI 480
           VALPLG+VLGFKVGVGLGGLLIGFLVG+F CL+LLMVFV RIDWGKEAQRAQLMAKDGEI
Sbjct: 421 VALPLGMVLGFKVGVGLGGLLIGFLVGVFVCLILLMVFVWRIDWGKEAQRAQLMAKDGEI 480

Query: 481 -VVDDVK 487
            VVD+VK
Sbjct: 481 VVVDNVK 486

BLAST of Lsi09G003660.1 vs. ExPASy TrEMBL
Match: A0A6J1KAE5 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111491567 PE=3 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 2.8e-213
Identity = 393/484 (81.20%), Postives = 435/484 (89.88%), Query Frame = 0

Query: 3   VSASSSTLESGS-RPRITKWVSKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITTAF 62
           +S SSSTLESG      TK +S+D TN I+SELKLQR +ALPLIAMNLTWFVKI ITTAF
Sbjct: 1   MSVSSSTLESGPLTSATTKRISQDFTNDIISELKLQRTIALPLIAMNLTWFVKITITTAF 60

Query: 63  LGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 122
           LGRLG+LPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL
Sbjct: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120

Query: 123 LLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQT 182
           LLLAT+PISFLWLNVD ILI+FGQ+KDISLAAK+YL YLLPDLV+TSFLCPLKSYLSS+T
Sbjct: 121 LLLATIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180

Query: 183 ETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMSN 242
           ETLPIM++S++ALALH+PINIFLAKSKGLIGVS+AIWVTDFVAMISLA+YV VK+R    
Sbjct: 181 ETLPIMISSSMALALHIPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKER---- 240

Query: 243 NGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVL 302
              +GGWFDQTV DW+RL KLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVL
Sbjct: 241 -NREGGWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVL 300

Query: 303 NFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGRG 362
           NFDYLL++VMLSLATC SARVSNELG NR  +AR SAGVSVV SV FGL+GAA MVA RG
Sbjct: 301 NFDYLLFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLVGAATMVAARG 360

Query: 363 EWGRIFTKDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYGV 422
           EWG+IF+KDEG +RMV+KMLVLMAAIEVVN+P+AVCGG+VRG G+PLMGLCA+LGGFYGV
Sbjct: 361 EWGKIFSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGV 420

Query: 423 ALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKD---G 482
           ALPLG++LGFKVG GLGGLL+GFLVG+FGCL  L+VFV RIDW KE  +A  MA     G
Sbjct: 421 ALPLGLILGFKVGAGLGGLLMGFLVGVFGCLGSLLVFVWRIDWEKEGLKASAMASGQAIG 479

BLAST of Lsi09G003660.1 vs. ExPASy TrEMBL
Match: A0A6J1GZZ2 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111459195 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 3.7e-213
Identity = 393/489 (80.37%), Postives = 434/489 (88.75%), Query Frame = 0

Query: 3   VSASSSTLESGS-RPRITKWVSKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITTAF 62
           +S S STLESG      TKWVS+D TN I+SELKLQR +ALPLI MNLTWFVKI ITTAF
Sbjct: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60

Query: 63  LGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 122
           LGRLG+LPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL
Sbjct: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120

Query: 123 LLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQT 182
           LLL T+PISFLWLNVD ILI+FGQ+KDISLAAK+YL YLLPDLV+TSFLCPLKSYLSS+T
Sbjct: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180

Query: 183 ETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMSN 242
           ETLPIM++S++ALALHVPINIFLAKSKGLIGVS+AIWVTDFVAMISLA+YV VK+     
Sbjct: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKE----- 240

Query: 243 NGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVL 302
              +GGWFDQTV DW+RL KLSGPCCLTTCLEWWCYEILILLTGRL NAKQAVGTIAIVL
Sbjct: 241 TNREGGWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVL 300

Query: 303 NFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGRG 362
           NFDYLL++VMLSLATC SARVSNELG NR  +AR SAGVSVV SV FGL+GAA MVA RG
Sbjct: 301 NFDYLLFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARG 360

Query: 363 EWGRIFTKDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYGV 422
           EWG+IF+KDEG +RMV+KMLVLMAAIEVVN+P+AVCGG+VRG G+PLMGLCA+LGGFYGV
Sbjct: 361 EWGKIFSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGV 420

Query: 423 ALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAK---DG 482
           ALPLG++LGFKVG GLGGLL+GFLVG+FGCL LL+ FV RIDW KE  +A  MA     G
Sbjct: 421 ALPLGLILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATG 480

Query: 483 EIVVDDVKI 488
           E+VV DV +
Sbjct: 481 EVVVADVDV 484

BLAST of Lsi09G003660.1 vs. NCBI nr
Match: XP_038875396.1 (protein DETOXIFICATION 56 [Benincasa hispida])

HSP 1 Score: 860.1 bits (2221), Expect = 9.0e-246
Identity = 456/488 (93.44%), Postives = 467/488 (95.70%), Query Frame = 0

Query: 1   MSVSASSSTLESGSRPRITKWVSKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITTA 60
           MSVS SSSTLESGS PRITKWVSKD TNSILSELKLQRG+ALPLIAMNLTWFVKIAITTA
Sbjct: 36  MSVSVSSSTLESGSSPRITKWVSKDFTNSILSELKLQRGIALPLIAMNLTWFVKIAITTA 95

Query: 61  FLGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIF 120
           FLGRLG+LPLA GTLGFTFANVTGFSVLNGLC AMEPICGQAFGAKNFKLLHKTLLMSIF
Sbjct: 96  FLGRLGDLPLAAGTLGFTFANVTGFSVLNGLCCAMEPICGQAFGAKNFKLLHKTLLMSIF 155

Query: 121 LLLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQ 180
           LLLLATLPISFLWLNVD ILI FGQQKDISLAAKTYL YLLPDLVITSFLCPLKSYLSSQ
Sbjct: 156 LLLLATLPISFLWLNVDSILIHFGQQKDISLAAKTYLLYLLPDLVITSFLCPLKSYLSSQ 215

Query: 181 TETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMS 240
           TETLPIML+SALALALHVP+NIFLAKSKGL GVS+AIW+TDFVAMISLAIYVW+KQ   S
Sbjct: 216 TETLPIMLSSALALALHVPVNIFLAKSKGLTGVSMAIWLTDFVAMISLAIYVWLKQ-SRS 275

Query: 241 NNGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300
           N+ E+GGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV
Sbjct: 276 NDEEEGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 335

Query: 301 LNFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGR 360
           LNFDYLLYSVMLSLATCASARVSNELGGNRG EARWSAGVSVVGSVVFGLMGAAAMVAGR
Sbjct: 336 LNFDYLLYSVMLSLATCASARVSNELGGNRGAEARWSAGVSVVGSVVFGLMGAAAMVAGR 395

Query: 361 GEWGRIFTKDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYG 420
           GEWGRIFT+DE VVRMVRKMLVLMAAIEVVNFPVAVCGGVVRG GKPLMGL AS+GGFYG
Sbjct: 396 GEWGRIFTRDEEVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGVGKPLMGLGASVGGFYG 455

Query: 421 VALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDGEI 480
           VALPLGVVLGFKVGVGL GLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLM KDGEI
Sbjct: 456 VALPLGVVLGFKVGVGLSGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMVKDGEI 515

Query: 481 -VVDDVKI 488
            VVDD KI
Sbjct: 516 AVVDDFKI 522

BLAST of Lsi09G003660.1 vs. NCBI nr
Match: XP_008437164.1 (PREDICTED: protein DETOXIFICATION 56 [Cucumis melo])

HSP 1 Score: 841.3 bits (2172), Expect = 4.3e-240
Identity = 448/487 (91.99%), Postives = 462/487 (94.87%), Query Frame = 0

Query: 1   MSVSASSSTLESGSRPRITKWVSKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITTA 60
           MSV+ SSSTLESGS PRITKWVSKD  NSILSELKLQRG+ALPLIAMNLTWFVKIAITTA
Sbjct: 1   MSVTVSSSTLESGSSPRITKWVSKDFINSILSELKLQRGIALPLIAMNLTWFVKIAITTA 60

Query: 61  FLGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIF 120
           FLGRLG+LPLA GTLGFTFANVTGFSVLNGLC AMEPICGQAFGAKNFKLLHKTL MSIF
Sbjct: 61  FLGRLGDLPLAAGTLGFTFANVTGFSVLNGLCCAMEPICGQAFGAKNFKLLHKTLFMSIF 120

Query: 121 LLLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQ 180
           LLLLATLPISFLWLNVD ILI FGQQKDIS+AAKTYLFYLLPDL+ITSFLCPLKSYLSSQ
Sbjct: 121 LLLLATLPISFLWLNVDTILIHFGQQKDISIAAKTYLFYLLPDLLITSFLCPLKSYLSSQ 180

Query: 181 TETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMS 240
           TETLP ML+SALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVW+KQ  MS
Sbjct: 181 TETLPTMLSSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWLKQ-SMS 240

Query: 241 NNGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300
           NN E GGWFDQTVQDWVRL+KLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV
Sbjct: 241 NNEEGGGWFDQTVQDWVRLLKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300

Query: 301 LNFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGR 360
           LNFDYLLYSVMLSLATCASARVSNELG N G+ ARWSAGVSVVGSVV GLMGAAAMVAGR
Sbjct: 301 LNFDYLLYSVMLSLATCASARVSNELGRNSGLAARWSAGVSVVGSVVVGLMGAAAMVAGR 360

Query: 361 GEWGRIFTKDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYG 420
           GEWGRIFT+DE VVRMV+KMLVLMAAIEVVNFPVAVCGGVVRG GKPLMGL ASLGGFYG
Sbjct: 361 GEWGRIFTRDEEVVRMVKKMLVLMAAIEVVNFPVAVCGGVVRGVGKPLMGLGASLGGFYG 420

Query: 421 VALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDGE- 480
           VALPLGVVLGFKVGVGLGGLLIGFLVG+F CLVLLMVFV RIDWGKEAQ AQLMAKDGE 
Sbjct: 421 VALPLGVVLGFKVGVGLGGLLIGFLVGVFVCLVLLMVFVWRIDWGKEAQAAQLMAKDGEV 480

Query: 481 IVVDDVK 487
           +VVD+VK
Sbjct: 481 VVVDNVK 486

BLAST of Lsi09G003660.1 vs. NCBI nr
Match: KAA0042839.1 (protein DETOXIFICATION 56 [Cucumis melo var. makuwa])

HSP 1 Score: 840.1 bits (2169), Expect = 9.6e-240
Identity = 447/487 (91.79%), Postives = 462/487 (94.87%), Query Frame = 0

Query: 1   MSVSASSSTLESGSRPRITKWVSKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITTA 60
           MSV+ SSSTLESGS PRITKWVSKD  NSILSELKLQRG+ALPLIAMNLTWFVKIAITTA
Sbjct: 1   MSVTVSSSTLESGSSPRITKWVSKDFINSILSELKLQRGIALPLIAMNLTWFVKIAITTA 60

Query: 61  FLGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIF 120
           FLGRLG+LPLA GTLGFTFANVTGFSVLNGLC AMEPICGQAFGAKNFKLLHKTL MSIF
Sbjct: 61  FLGRLGDLPLAAGTLGFTFANVTGFSVLNGLCCAMEPICGQAFGAKNFKLLHKTLFMSIF 120

Query: 121 LLLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQ 180
           LLLLATLPISFLWLNVD ILI FGQQKDIS+AAKTYLFYLLPDL+ITSFLCPLKSYLSSQ
Sbjct: 121 LLLLATLPISFLWLNVDTILIHFGQQKDISIAAKTYLFYLLPDLLITSFLCPLKSYLSSQ 180

Query: 181 TETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMS 240
           TETLP ML+SALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVW+KQ  MS
Sbjct: 181 TETLPTMLSSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWLKQ-SMS 240

Query: 241 NNGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300
           NN E GGWFDQTVQDWVRL+KLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV
Sbjct: 241 NNEEGGGWFDQTVQDWVRLLKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300

Query: 301 LNFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGR 360
           LNFDYLLYSVMLSLATCASARVSNELG N G+ ARWSAGVSVVGSVV GLMGAAAMVAGR
Sbjct: 301 LNFDYLLYSVMLSLATCASARVSNELGRNSGLAARWSAGVSVVGSVVVGLMGAAAMVAGR 360

Query: 361 GEWGRIFTKDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYG 420
           GEWGRIFT+DE VVRMV+KMLVLMAAIEVVNFPVAVCGGVVRG GKPLMGL ASLGGFYG
Sbjct: 361 GEWGRIFTRDEEVVRMVKKMLVLMAAIEVVNFPVAVCGGVVRGVGKPLMGLGASLGGFYG 420

Query: 421 VALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDGE- 480
           VALPLG+VLGFKVGVGLGGLLIGFLVG+F CLVLLMVFV RIDWGKEAQ AQLMAKDGE 
Sbjct: 421 VALPLGMVLGFKVGVGLGGLLIGFLVGVFVCLVLLMVFVWRIDWGKEAQAAQLMAKDGEV 480

Query: 481 IVVDDVK 487
           +VVD+VK
Sbjct: 481 VVVDNVK 486

BLAST of Lsi09G003660.1 vs. NCBI nr
Match: XP_004147605.1 (protein DETOXIFICATION 56 [Cucumis sativus])

HSP 1 Score: 840.1 bits (2169), Expect = 9.6e-240
Identity = 444/487 (91.17%), Postives = 464/487 (95.28%), Query Frame = 0

Query: 1   MSVSASSSTLESGSRPRITKWVSKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITTA 60
           MSV+ SSSTLESGS PRITKWVSKD  NSI+SELKLQRG+ALPL+AMNLTWFVKIAITTA
Sbjct: 1   MSVTVSSSTLESGSSPRITKWVSKDFINSIISELKLQRGIALPLVAMNLTWFVKIAITTA 60

Query: 61  FLGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIF 120
           FLGRLG+LPLA GTLGFTFANVTGFSVLNGLC AMEPICGQAFGAKNF+LLHKTL MSIF
Sbjct: 61  FLGRLGDLPLAAGTLGFTFANVTGFSVLNGLCCAMEPICGQAFGAKNFQLLHKTLFMSIF 120

Query: 121 LLLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQ 180
           LLLLATLPISFLWLNVD ILI FGQQKD+S+AAKTYLFYLLPDL+ITSFLCPLKSYLSSQ
Sbjct: 121 LLLLATLPISFLWLNVDTILIHFGQQKDLSIAAKTYLFYLLPDLLITSFLCPLKSYLSSQ 180

Query: 181 TETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMS 240
           TETLPIML+SALALALHVPIN+FLAKSKGLIGVSIAIWVTDFVAMISLAIYVW+KQ  MS
Sbjct: 181 TETLPIMLSSALALALHVPINLFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWLKQ-SMS 240

Query: 241 NNGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300
           NN E GGWFDQTVQDWVRL+KLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV
Sbjct: 241 NNEEGGGWFDQTVQDWVRLLKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300

Query: 301 LNFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGR 360
           LNFDYLLYSVMLSLATCASARVSNELG N  + ARWSAGVSVVGSVV GLMGAAAMVAGR
Sbjct: 301 LNFDYLLYSVMLSLATCASARVSNELGRNSELAARWSAGVSVVGSVVVGLMGAAAMVAGR 360

Query: 361 GEWGRIFTKDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYG 420
           GEWGRIFT+DEGVVRMV+KMLVLMAAIEVVNFPVAVCGGVVRG GKPLMGL ASLGGFYG
Sbjct: 361 GEWGRIFTRDEGVVRMVKKMLVLMAAIEVVNFPVAVCGGVVRGVGKPLMGLGASLGGFYG 420

Query: 421 VALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDGEI 480
           VALPLG+VLGFKVGVGLGGLLIGFLVG+F CL+LLMVFV RIDWGKEAQRAQLMAKDGEI
Sbjct: 421 VALPLGMVLGFKVGVGLGGLLIGFLVGVFVCLILLMVFVWRIDWGKEAQRAQLMAKDGEI 480

Query: 481 -VVDDVK 487
            VVD+VK
Sbjct: 481 VVVDNVK 486

BLAST of Lsi09G003660.1 vs. NCBI nr
Match: KAE8647882.1 (hypothetical protein Csa_000448 [Cucumis sativus])

HSP 1 Score: 770.4 bits (1988), Expect = 9.3e-219
Identity = 415/487 (85.22%), Postives = 435/487 (89.32%), Query Frame = 0

Query: 1   MSVSASSSTLESGSRPRITKWVSKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITTA 60
           MSV+ SSSTLESGS PRITKWVSKD  NSI+SELKLQRG+ALPL+AMNLTWFVKIAITTA
Sbjct: 1   MSVTVSSSTLESGSSPRITKWVSKDFINSIISELKLQRGIALPLVAMNLTWFVKIAITTA 60

Query: 61  FLGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIF 120
           FLGRLG+LPLA GTLGFTFANVTGFSVLNGLC AMEPICGQAFGAKNF+LLHKTL MSIF
Sbjct: 61  FLGRLGDLPLAAGTLGFTFANVTGFSVLNGLCCAMEPICGQAFGAKNFQLLHKTLFMSIF 120

Query: 121 LLLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQ 180
           LLLLATLPISFLWLNVD ILI FGQQKD+S+AAKTYLFYLLPDL+ITSFLCPLKSYLSSQ
Sbjct: 121 LLLLATLPISFLWLNVDTILIHFGQQKDLSIAAKTYLFYLLPDLLITSFLCPLKSYLSSQ 180

Query: 181 TETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMS 240
           TETLPIML+SALALALHVPIN+FLAKSKGLIGVSIAIWVTDFVAMISLAIYVW+KQ  MS
Sbjct: 181 TETLPIMLSSALALALHVPINLFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWLKQ-SMS 240

Query: 241 NNGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300
           NN E GGWFDQTVQDWVRL+KLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV
Sbjct: 241 NNEEGGGWFDQTVQDWVRLLKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIV 300

Query: 301 LNFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGR 360
           LNFDYLLYSVMLSLATCASARVSNELG N  + A                          
Sbjct: 301 LNFDYLLYSVMLSLATCASARVSNELGRNSELAA-------------------------- 360

Query: 361 GEWGRIFTKDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYG 420
               RIFT+DEGVVRMV+KMLVLMAAIEVVNFPVAVCGGVVRG GKPLMGL ASLGGFYG
Sbjct: 361 ----RIFTRDEGVVRMVKKMLVLMAAIEVVNFPVAVCGGVVRGVGKPLMGLGASLGGFYG 420

Query: 421 VALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDGEI 480
           VALPLG+VLGFKVGVGLGGLLIGFLVG+F CL+LLMVFV RIDWGKEAQRAQLMAKDGEI
Sbjct: 421 VALPLGMVLGFKVGVGLGGLLIGFLVGVFVCLILLMVFVWRIDWGKEAQRAQLMAKDGEI 456

Query: 481 -VVDDVK 487
            VVD+VK
Sbjct: 481 VVVDNVK 456

BLAST of Lsi09G003660.1 vs. TAIR 10
Match: AT4G22790.1 (MATE efflux family protein )

HSP 1 Score: 533.5 bits (1373), Expect = 1.8e-151
Identity = 280/481 (58.21%), Postives = 360/481 (74.84%), Query Frame = 0

Query: 1   MSVSASSSTLESGSRPRITKWV-SKDLTNSILSELKLQRGLALPLIAMNLTWFVKIAITT 60
           MS ++ S +L+    P +++ + SK L  SI+ ELKLQ  + LPL+ MNL WF K+  T+
Sbjct: 1   MSETSKSESLD----PEVSEGLCSKTLMQSIVHELKLQMRIGLPLVVMNLLWFGKMTTTS 60

Query: 61  AFLGRLGELPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSI 120
            FLGR GEL LAGG+LGF+FANVTGFSVL G+  AMEPICGQAFGAKNFKLLHKTL M++
Sbjct: 61  VFLGRQGELNLAGGSLGFSFANVTGFSVLYGISAAMEPICGQAFGAKNFKLLHKTLFMAV 120

Query: 121 FLLLLATLPISFLWLNVDKILIKFGQQKDISLAAKTYLFYLLPDLVITSFLCPLKSYLSS 180
            LLLL ++PISFLWLNV KIL  FGQ++DIS  AK YL YLLP+L I SFLCPLK+YLSS
Sbjct: 121 LLLLLISVPISFLWLNVHKILTGFGQREDISFIAKKYLLYLLPELPILSFLCPLKAYLSS 180

Query: 181 QTETLPIMLTSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWVKQRMM 240
           Q  TLPIM T+A A +LH+PINI L+K++G+ GV++A+W+TDF+ +I L  YV V +RM 
Sbjct: 181 QGVTLPIMFTTAAATSLHIPINIVLSKARGIEGVAMAVWITDFIVVILLTGYVIVVERMK 240

Query: 241 SNNGEKGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAI 300
            N  ++GGW +Q+ QDW+ L+KLSGPCCLT CLEWWCYEIL+LLTGRLPN  QAV  + I
Sbjct: 241 ENKWKQGGWLNQSAQDWLTLIKLSGPCCLTVCLEWWCYEILVLLTGRLPNPVQAVSILII 300

Query: 301 VLNFDYLLYSVMLSLATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAG 360
           V NFDYLLY+VMLSL TC + RVSNELG N    A  +A  +++  ++ G +GA  M+A 
Sbjct: 301 VFNFDYLLYAVMLSLGTCVATRVSNELGANNPKGAYRAAYTTLIVGIISGCIGALVMIAF 360

Query: 361 RGEWGRIFT-KDEGVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGF 420
           RG WG ++T  D+ ++  V+KM+++MA IEVVNFP+ VCG +VRG  KP +G+ A+L GF
Sbjct: 361 RGFWGSLYTHHDQLILNGVKKMMLIMAVIEVVNFPLMVCGEIVRGTAKPSLGMYANLSGF 420

Query: 421 YGVALPLGVVLGFKVGVGLGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDG 480
           Y +ALPLG  L FK   GL G LIG  VG+  CL +L++F+ RIDW KEA +AQ++  + 
Sbjct: 421 YLLALPLGATLAFKAKQGLQGFLIGLFVGISLCLSILLIFIARIDWEKEAGKAQILTCNT 477

BLAST of Lsi09G003660.1 vs. TAIR 10
Match: AT4G29140.1 (MATE efflux family protein )

HSP 1 Score: 299.3 bits (765), Expect = 5.7e-81
Identity = 180/460 (39.13%), Postives = 260/460 (56.52%), Query Frame = 0

Query: 31  LSELKLQRGLALPLIAMNLTWFVKIAITTAFLGRLGELPLAGGTLGFTFANVTGFSVLNG 90
           ++E K    LA P+    L  +++ A++  FLG+LG+L LA G+L   FAN+TG+SVL+G
Sbjct: 54  VTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVLSG 113

Query: 91  LCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLATLPISFLWLNVDKILIKFGQQKDIS 150
           L   MEP+C QAFGA  FKLL  TL  ++  LL+  +PIS LW NV KI +   Q  DI+
Sbjct: 114 LALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPDIA 173

Query: 151 LAAKTYLFYLLPDLVITSFLCPLKSYLSSQTETLPIMLTSALALALHVPINIFLAK--SK 210
             A+TYL + LPDL+  + L P++ YL +Q    P+ L S      H+P N+FL      
Sbjct: 174 KLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYLRL 233

Query: 211 GLIGVSIAIWVTDFVAMISLAIYVWVKQRMMSNNGEKGGWFDQT---VQDWVRLVKLSGP 270
           GL GV++A  +T+   +  L  YVW      ++      W D T    + W  L++L+GP
Sbjct: 234 GLTGVAVASSITNIFVVAFLVCYVW------ASGLHAPTWTDPTRDCFRGWAPLLRLAGP 293

Query: 271 CCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLNFDYLLYSVMLSLATCASARVSNE 330
            C++ CLEWW YEI+I+L G L N +  V  + +++     LY    SL+   S RV NE
Sbjct: 294 SCVSVCLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNE 353

Query: 331 LGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGRGEWGRIFTKDEGVVRMVRKMLVLMA 390
           LG NR   A+ +A V++V + V G++ AA   + R  WGRIFT D+ ++++    L ++ 
Sbjct: 354 LGANRPKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILG 413

Query: 391 AIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYGVALPLGVVLGFKVGVGLGGLLIGFL 450
             E+ N P  V  GVVRG  +P      +LG FY V +P+ V LGF  G+G  GL +G L
Sbjct: 414 LCEIGNCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLL 473

Query: 451 VGMFGCLVLLMVFVGRIDWGKEAQRAQLMAKDGEIVVDDV 486
                C  L+M  VG  DW  EA++AQ +    E V +D+
Sbjct: 474 AAQISCAGLMMYVVGTTDWESEAKKAQTLT-CAETVENDI 506

BLAST of Lsi09G003660.1 vs. TAIR 10
Match: AT1G71870.1 (MATE efflux family protein )

HSP 1 Score: 286.6 bits (732), Expect = 3.8e-77
Identity = 177/459 (38.56%), Postives = 264/459 (57.52%), Query Frame = 0

Query: 30  ILSELKLQRGLALPLIAMNLTWFVKIAITTAFLGRLGELPLAGGTLGFTFANVTGFSVLN 89
           ++ ELK    + LP+ AMN   +V+  ++  FLGRLG L LAGG L   F N+TG+SV+ 
Sbjct: 21  VIEELKELWAMVLPITAMNCLVYVRAVVSVLFLGRLGSLELAGGALSIGFTNITGYSVMV 80

Query: 90  GLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLATLPISFLWLNVDKILIKFGQQKDI 149
           GL   +EP+C QA+G+KN+ LL  +L   + +LL+A+LPIS LW+N+  I++  GQ  +I
Sbjct: 81  GLASGLEPVCSQAYGSKNWDLLTLSLHRMVVILLMASLPISLLWINLGPIMLFMGQNPEI 140

Query: 150 SLAAKTYLFYLLPDLVITSFLCPLKSYLSSQTETLPIMLTSALALALHVPINIFLAKSK- 209
           +  A  Y  Y LPDL+  + L PL+ YL SQ  T P+M  +  A+A HVP+N +L   K 
Sbjct: 141 TATAAEYCLYALPDLLTNTLLQPLRVYLRSQRVTKPMMWCTLAAVAFHVPLNYWLVMVKH 200

Query: 210 -GLIGVSIAIWVTDFVAMISLAIYVWVK---QRMMSNNGEKGGWF-------DQTVQDWV 269
            G+ GV+IA  VT+ + ++ L  YVWV    Q+ +S +G+ G            +V + V
Sbjct: 201 WGVPGVAIASVVTNLIMVVLLVGYVWVSGMLQKRVSGDGDGGSTTMVAVVAQSSSVMELV 260

Query: 270 ----RLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLNFDYLLYSVMLS 329
                L++++ P CL  CLEWW YEI+I++ G L N K AV    I++    L+Y+V ++
Sbjct: 261 GGLGPLMRVAVPSCLGICLEWWWYEIVIVMGGYLENPKLAVAATGILIQTTSLMYTVPMA 320

Query: 330 LATCASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGRGEWGRIFTKDEGV 389
           LA C SARV NELG  R  +AR +A V++  + V G +  A  V  +  W  +FT  E +
Sbjct: 321 LAGCVSARVGNELGAGRPYKARLAANVALACAFVVGALNVAWTVILKERWAGLFTGYEPL 380

Query: 390 VRMVRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYGVALPLGVVLGFKV 449
             +V  ++ ++   E+ N P     G++RG G+P +G   +LG FY V  P+ V L F +
Sbjct: 381 KVLVASVMPIVGLCELGNCPQTTGCGILRGTGRPAVGAHVNLGSFYFVGTPVAVGLAFWL 440

Query: 450 GVGLGGLLIGFLVGMFGCLV-LLMVFVGRIDWGKEAQRA 472
            +G  GL  G L     C+V +L   + R DW  EA +A
Sbjct: 441 KIGFSGLWFGLLSAQAACVVSILYAVLARTDWEGEAVKA 479

BLAST of Lsi09G003660.1 vs. TAIR 10
Match: AT5G49130.1 (MATE efflux family protein )

HSP 1 Score: 285.4 bits (729), Expect = 8.5e-77
Identity = 169/455 (37.14%), Postives = 257/455 (56.48%), Query Frame = 0

Query: 30  ILSELKLQRGLALPLIAMNLTWFVKIAITTAFLGRLGELPLAGGTLGFTFANVTGFSVLN 89
           ++ ELK    ++ P+ AM++  ++K   +   +GRLG L LAGG L   F N+TG+SVL+
Sbjct: 23  VVEELKRIWDISFPVAAMSILNYLKNMTSVVCMGRLGSLELAGGALAIGFTNITGYSVLS 82

Query: 90  GLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLATLPISFLWLNVDKILIKFGQQKDI 149
           GL   MEP+CGQA G+KN  L   TL  +IFLLLLA+LPIS LWLN+  +++   QQ DI
Sbjct: 83  GLATGMEPLCGQAIGSKNPSLASLTLKRTIFLLLLASLPISLLWLNLAPLMLMLRQQHDI 142

Query: 150 SLAAKTYLFYLLPDLVITSFLCPLKSYLSSQTETLPIMLTSALALALHVPINIFLA--KS 209
           +  A  Y  + LPDL+  SFL PL+ YL  +  T P+M  + +++ LH+PI  F     S
Sbjct: 143 TRVASLYCSFSLPDLLANSFLHPLRIYLRCKGTTWPLMWCTLVSVLLHLPITAFFTFYIS 202

Query: 210 KGLIGVSIAIWVTDFVAMISLAIYVWVKQRMMSNNGEKG----------GWFDQTVQD-W 269
            G+ GV+++ ++T+F+++  L  Y++++         K           G  D    D W
Sbjct: 203 LGVPGVAVSSFLTNFISLSLLLCYIYLENNNNDKTTSKSLCLDTPLMLYGSRDSGENDVW 262

Query: 270 VRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLNFDYLLYSVMLSLAT 329
             LVK + P C+  CLEWW YE + +L G LP  K A+   AIV+    L+Y++  +L+ 
Sbjct: 263 STLVKFAVPSCIAVCLEWWWYEFMTVLAGYLPEPKVALAAAAIVIQTTSLMYTIPTALSA 322

Query: 330 CASARVSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGRGEWGRIFTKDEGVVRM 389
             S RVSNELG  R  +A+ +A V+V  +V   + G      GR  WG++FT D+ V+ +
Sbjct: 323 AVSTRVSNELGAGRPEKAKTAATVAVGAAVAVSVFGLVGTTVGREAWGKVFTADKVVLEL 382

Query: 390 VRKMLVLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYGVALPLGVVLGFKVGVG 449
              ++ ++ A E+ N P  +  G++RG+ +P +G   +   FY V  P+ VVL F  G+G
Sbjct: 383 TAAVIPVIGACELANCPQTISCGILRGSARPGIGAKINFYAFYVVGAPVAVVLAFVWGLG 442

Query: 450 LGGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRA 472
             GL  G L     C + ++  V   DW KE+ +A
Sbjct: 443 FMGLCYGLLGAQLACAISILTVVYNTDWNKESLKA 477

BLAST of Lsi09G003660.1 vs. TAIR 10
Match: AT4G23030.1 (MATE efflux family protein )

HSP 1 Score: 278.9 bits (712), Expect = 7.9e-75
Identity = 185/464 (39.87%), Postives = 271/464 (58.41%), Query Frame = 0

Query: 28  NSILSELKLQRGLALPLIAMNLTWFVKIAITTAFLGRLGEL-PLAGGTLGFTFANVTGFS 87
           +S + E K    ++LPLI   L  + +  I+  FLGRL +L  L+GG+L   FAN+TG+S
Sbjct: 27  SSSIQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGYS 86

Query: 88  VLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLATLPISFLWLNVDKILIKFGQQ 147
           +L+GL   MEPIC QAFGAK FKLL   L  +  LLLL +LPIS LWLN+ KIL+ FGQ 
Sbjct: 87  LLSGLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQD 146

Query: 148 KDISLAAKTYLFYLLPDLVITSFLCPLKSYLSSQTETLPIMLTSALALALHVPINIFLAK 207
           ++IS  A+ ++ + LPDL++ SFL P++ YL SQ+ TLP+  ++  A+ LH+PIN  L  
Sbjct: 147 EEISNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLVS 206

Query: 208 S--KGLIGVSI-AIWVTDFVAMISLAIYVWVKQRMMSNNGEK--GGWFDQTVQDWVRLVK 267
           S   GL GV++ AIW T+   +  L IY+     + S   +K  GG+     + W  L+K
Sbjct: 207 SLGLGLKGVALGAIW-TNVNLLGFLIIYI-----VFSGVYQKTWGGFSMDCFKGWRSLMK 266

Query: 268 LSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLNFDYLLYSVMLSLATCASAR 327
           L+ P C++ CLEWW YEI+ILL G L N +  V ++ I++    L+Y    SL+   S R
Sbjct: 267 LAIPSCVSVCLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTR 326

Query: 328 VSNELGGNRGVEARWSAGVSVVGSVVFGLMGAAAMVAGRGEWGRIFTKDEGVVRMVRKML 387
           V NELG N+  +AR +A   +  S+  GL+     +  R  W R+FT +E +V++   +L
Sbjct: 327 VGNELGANQPDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVL 386

Query: 388 VLMAAIEVVNFPVAVCGGVVRGAGKPLMGLCASLGGFYGVALPLGVVLGFKVGVGLGGLL 447
            ++   E+ N P     GV+RG+ +P +G   +L  FY V +P+ V L F  G    GL 
Sbjct: 387 PIIGLCELGNCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLW 446

Query: 448 IGFLVGMFGCLVLLMVFVGRIDWGKEAQRA-QLMAKDGEIVVDD 485
           +G       CL+ ++V + R DW  E  RA +LM +  +   DD
Sbjct: 447 LGLFAAQGSCLISMLVVLARTDWEVEVHRAKELMTRSCDGDEDD 484

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O496602.5e-15058.21Protein DETOXIFICATION 56 OS=Arabidopsis thaliana OX=3702 GN=DTX56 PE=1 SV=1[more]
Q9SZE28.0e-8039.13Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1[more]
Q9LE205.4e-7638.56Protein DETOXIFICATION 54 OS=Arabidopsis thaliana OX=3702 GN=DTX54 PE=2 SV=1[more]
Q9FH211.2e-7537.14Protein DETOXIFICATION 55 OS=Arabidopsis thaliana OX=3702 GN=DTX55 PE=2 SV=1[more]
O827521.1e-7339.87Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3ATW52.1e-24091.99Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103482671 PE=3 SV=1[more]
A0A5A7TN934.6e-24091.79Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold4... [more]
A0A0A0KMR74.6e-24091.17Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G157920 PE=3 SV=1[more]
A0A6J1KAE52.8e-21381.20Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111491567 PE=3 SV=1[more]
A0A6J1GZZ23.7e-21380.37Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111459195 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038875396.19.0e-24693.44protein DETOXIFICATION 56 [Benincasa hispida][more]
XP_008437164.14.3e-24091.99PREDICTED: protein DETOXIFICATION 56 [Cucumis melo][more]
KAA0042839.19.6e-24091.79protein DETOXIFICATION 56 [Cucumis melo var. makuwa][more]
XP_004147605.19.6e-24091.17protein DETOXIFICATION 56 [Cucumis sativus][more]
KAE8647882.19.3e-21985.22hypothetical protein Csa_000448 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT4G22790.11.8e-15158.21MATE efflux family protein [more]
AT4G29140.15.7e-8139.13MATE efflux family protein [more]
AT1G71870.13.8e-7738.56MATE efflux family protein [more]
AT5G49130.18.5e-7737.14MATE efflux family protein [more]
AT4G23030.17.9e-7539.87MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 265..427
e-value: 2.6E-26
score: 92.2
coord: 43..202
e-value: 1.9E-30
score: 105.6
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 44..440
e-value: 1.4E-50
score: 170.3
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 23..477
NoneNo IPR availablePANTHERPTHR11206:SF93PROTEIN DETOXIFICATION 56coord: 23..477
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 33..470
e-value: 3.99781E-113
score: 339.163

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi09G003660Lsi09G003660gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi09G003660.1.exon.1Lsi09G003660.1.exon.1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi09G003660.1.CDS.1Lsi09G003660.1.CDS.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi09G003660.1Lsi09G003660.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity