Lsi06G009570.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G009570.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionChaperone protein dnaJ 49-like
Locationchr06: 19497212 .. 19498294 (+)
Sequence length1083
RNA-Seq ExpressionLsi06G009570.1
SyntenyLsi06G009570.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGGGAACAAGGATGATGCTTTGAAATGCTTGAGAATTGGAAAAGAGGCTTTGGAAGCTGGGAATTTGGTCCGAGCCCTAAAATTTGTCAACAAAGCTCGTCGTCTTGATCCGGCGCTTCCCGTCGACGACCTCTTACCGGAACTTAAAAACGTTTCTGGTGATCAGTTGGGTGGTGATGCGTGTGAAACTGGCAATGGGTCACCTGGGTCCTCTCCATCCAAGTCCCCTGAACAGCCTTCGGTTCGCCGTAGAGTTCCGGCATCTGGGTCTTCTGAATCTTCAACCTCGGCGACTTATACTGAGGAGCATGTAGAGATTGTAAGGCAGATCAAGAAGAAGAAGGATTACTATGAGATTTTGGGATTGGAGAAGAGTTGCACTATTGAGGATGTTCGGAAGGCATATAGAAAGCTGTCTTTAAAGGTCCATCCAGATAAGAATAATGCTCCTGGAGCTGAAGAAGCATTTAAGGCTGTATCAAAGGCCTTTCAGTGTTTAAGTAACGAGGAGAGTAGAAAAAGGTATGATCTTGTCGGGTCCGAGGAACAAGTCTATGAGAGACGGACTGCAAGACATGGTGCAAATGGATTTAATGGGTTTTATGAAGGTGATGTTGATGCAGAGGAGATATTTAGAAACTTCTTCTTTGGTGGAATGCCAGCGACAACTACTCATTTCCGTAGTTTCAATTTTGGCCCTGGAATGGCACCTAGAACAGGGGATCATGGCTCTGGTGGTGGCCTTCGGACACTGATTCAATTGCTTCCTGTGCTGGTGATATTGCTTTTGAACTTCATGCCATCCTCAGAACCTATCTATTCTCTTTCACGTTCATATCCTTATGAACACCGATTTACAACTCAGAAGGGTGTCAATTATTATGTGAAATCAAAAAACTTTGAACAAGATTTTCCACCTAATAGTCGGGATCGTCTGGTACTTGAGCAAAGGGTAGATAGGGAATACTTCAATATTCTTGCTCAGAATTGTCGACTTGAGGTGCAACGACGCCAGTGGGGATTCATTCGTGAAACTCCGCATTGTGATATGTTGAATAAGTTTCAATCAACGGCTTGA

mRNA sequence

ATGGATGGGAACAAGGATGATGCTTTGAAATGCTTGAGAATTGGAAAAGAGGCTTTGGAAGCTGGGAATTTGGTCCGAGCCCTAAAATTTGTCAACAAAGCTCGTCGTCTTGATCCGGCGCTTCCCGTCGACGACCTCTTACCGGAACTTAAAAACGTTTCTGGTGATCAGTTGGGTGGTGATGCGTGTGAAACTGGCAATGGGTCACCTGGGTCCTCTCCATCCAAGTCCCCTGAACAGCCTTCGGTTCGCCGTAGAGTTCCGGCATCTGGGTCTTCTGAATCTTCAACCTCGGCGACTTATACTGAGGAGCATGTAGAGATTGTAAGGCAGATCAAGAAGAAGAAGGATTACTATGAGATTTTGGGATTGGAGAAGAGTTGCACTATTGAGGATGTTCGGAAGGCATATAGAAAGCTGTCTTTAAAGGTCCATCCAGATAAGAATAATGCTCCTGGAGCTGAAGAAGCATTTAAGGCTGTATCAAAGGCCTTTCAGTGTTTAAGTAACGAGGAGAGTAGAAAAAGGTATGATCTTGTCGGGTCCGAGGAACAAGTCTATGAGAGACGGACTGCAAGACATGGTGCAAATGGATTTAATGGGTTTTATGAAGGTGATGTTGATGCAGAGGAGATATTTAGAAACTTCTTCTTTGGTGGAATGCCAGCGACAACTACTCATTTCCGTAGTTTCAATTTTGGCCCTGGAATGGCACCTAGAACAGGGGATCATGGCTCTGGTGGTGGCCTTCGGACACTGATTCAATTGCTTCCTGTGCTGGTGATATTGCTTTTGAACTTCATGCCATCCTCAGAACCTATCTATTCTCTTTCACGTTCATATCCTTATGAACACCGATTTACAACTCAGAAGGGTGTCAATTATTATGTGAAATCAAAAAACTTTGAACAAGATTTTCCACCTAATAGTCGGGATCGTCTGGTACTTGAGCAAAGGGTAGATAGGGAATACTTCAATATTCTTGCTCAGAATTGTCGACTTGAGGTGCAACGACGCCAGTGGGGATTCATTCGTGAAACTCCGCATTGTGATATGTTGAATAAGTTTCAATCAACGGCTTGA

Coding sequence (CDS)

ATGGATGGGAACAAGGATGATGCTTTGAAATGCTTGAGAATTGGAAAAGAGGCTTTGGAAGCTGGGAATTTGGTCCGAGCCCTAAAATTTGTCAACAAAGCTCGTCGTCTTGATCCGGCGCTTCCCGTCGACGACCTCTTACCGGAACTTAAAAACGTTTCTGGTGATCAGTTGGGTGGTGATGCGTGTGAAACTGGCAATGGGTCACCTGGGTCCTCTCCATCCAAGTCCCCTGAACAGCCTTCGGTTCGCCGTAGAGTTCCGGCATCTGGGTCTTCTGAATCTTCAACCTCGGCGACTTATACTGAGGAGCATGTAGAGATTGTAAGGCAGATCAAGAAGAAGAAGGATTACTATGAGATTTTGGGATTGGAGAAGAGTTGCACTATTGAGGATGTTCGGAAGGCATATAGAAAGCTGTCTTTAAAGGTCCATCCAGATAAGAATAATGCTCCTGGAGCTGAAGAAGCATTTAAGGCTGTATCAAAGGCCTTTCAGTGTTTAAGTAACGAGGAGAGTAGAAAAAGGTATGATCTTGTCGGGTCCGAGGAACAAGTCTATGAGAGACGGACTGCAAGACATGGTGCAAATGGATTTAATGGGTTTTATGAAGGTGATGTTGATGCAGAGGAGATATTTAGAAACTTCTTCTTTGGTGGAATGCCAGCGACAACTACTCATTTCCGTAGTTTCAATTTTGGCCCTGGAATGGCACCTAGAACAGGGGATCATGGCTCTGGTGGTGGCCTTCGGACACTGATTCAATTGCTTCCTGTGCTGGTGATATTGCTTTTGAACTTCATGCCATCCTCAGAACCTATCTATTCTCTTTCACGTTCATATCCTTATGAACACCGATTTACAACTCAGAAGGGTGTCAATTATTATGTGAAATCAAAAAACTTTGAACAAGATTTTCCACCTAATAGTCGGGATCGTCTGGTACTTGAGCAAAGGGTAGATAGGGAATACTTCAATATTCTTGCTCAGAATTGTCGACTTGAGGTGCAACGACGCCAGTGGGGATTCATTCGTGAAACTCCGCATTGTGATATGTTGAATAAGTTTCAATCAACGGCTTGA

Protein sequence

MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGGDACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYEILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLVGSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAPRTGDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQKGVNYYVKSKNFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQSTA
Homology
BLAST of Lsi06G009570.1 vs. ExPASy Swiss-Prot
Match: Q9FH28 (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 240.0 bits (611), Expect = 4.3e-62
Identity = 148/361 (41.00%), Postives = 214/361 (59.28%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDDA +CLRI ++A+ +G+  RALKF+N A+RL+P+L VD+L+    N+       
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVAACDNL------- 60

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
                       S S++       + +        +    YTEE+V++VR I +  DYY 
Sbjct: 61  -----------DSVSRNSSVSEKLKTMDGDDDKLETGKMKYTEENVDLVRNIIRNNDYYA 120

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGLEK+C+++++RKAYRKLSLKVHPDKN APG+EEAFK VSKAF CLS+  SR+++D V
Sbjct: 121 ILGLEKNCSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQV 180

Query: 181 GSEEQV----YERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPG 240
           G  ++        R  R   N  N F++ + D EEIFR  F  G       FR+ +    
Sbjct: 181 GIVDEFDHVQRRNRRPRRRYNTRNDFFDDEFDPEEIFRTVF--GQQREV--FRASHAYRT 240

Query: 241 MAPRT-----GDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQK 300
             PR        + +G    T+IQ+LP  ++LLL ++P SEP YSL ++  Y+   TTQ 
Sbjct: 241 RQPRNQFREEEINVAGPSCLTIIQILPFFLLLLLAYLPFSEPDYSLHKNQSYQIPKTTQN 300

Query: 301 -GVNYYVKSKN-FEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPH 351
             +++YV+S + F++ FP +S  R  LE  V +EY + L Q+CR+E+Q+R+W     TPH
Sbjct: 301 TEISFYVRSASAFDEKFPLSSSARANLEGNVIKEYKHFLFQSCRIELQKRRWNKKIPTPH 339

BLAST of Lsi06G009570.1 vs. ExPASy Swiss-Prot
Match: Q7ZXQ8 (DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.3e-47
Identity = 133/353 (37.68%), Postives = 193/353 (54.67%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           M+ N+D+A +C+RIGK A+EAG+  +A +F +KA RL P+     LL  L+         
Sbjct: 1   MESNRDEAERCVRIGKAAIEAGDKEKARRFFSKAERLYPSSEARVLLDALEKND------ 60

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
                GNG      SKS EQP       A   S   T   +T++ V+ V++IKK K YYE
Sbjct: 61  ---TAGNGPQSEKMSKSTEQPK------AEKDSSGDTGKGHTQDQVDGVQRIKKCKTYYE 120

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           +LG+      ED++KAYRKL+LK HPDKN+APGA EAFK +  A+  LSN E RK+YDL 
Sbjct: 121 VLGVSPDAGEEDLKKAYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDLT 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAPR 240
           GSE+ V  +   R+G   ++  +E D+  E++F  FF GG P+ + H  S N     +  
Sbjct: 181 GSEDNV--QNNHRNGGFDYHRGFEADITPEDLFNMFFGGGFPSGSVHTFS-NGRTRYSHH 240

Query: 241 TGDHGSG---------GGLRTLIQLLPVLVILLLNFMPS---SEPIYSL-SRSYPYEHRF 300
              H SG         GG    IQL+P++V++L++ +     S P YSL  RS     R 
Sbjct: 241 QHHHHSGHDREEERADGGFSMFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGQTIKRV 300

Query: 301 TTQKGVNYYVKSKNFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQ 341
           T    ++YYV SK+F+ ++  N      LE+ ++ +Y   +  NC  E Q++Q
Sbjct: 301 TENLQISYYV-SKDFKSEY--NGMLLQKLEKNIEEDYVANVRNNCWRERQQKQ 332

BLAST of Lsi06G009570.1 vs. ExPASy Swiss-Prot
Match: Q28I38 (DnaJ homolog subfamily B member 14 OS=Xenopus tropicalis OX=8364 GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 5.0e-47
Identity = 128/352 (36.36%), Postives = 194/352 (55.11%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           M+ N+D+A +C+RI K A+EAG+  +A +F++KA RL P+     LL   +         
Sbjct: 1   MESNRDEAERCVRIAKAAIEAGDKEKAKRFLSKAERLYPSSEARALLQAFEKND------ 60

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
                GNG   +  +K  EQP   +   AS SS+  T   +T++ ++ V++IKK K YYE
Sbjct: 61  ---TAGNGPQSAKMAKGTEQPKAEKDSNASASSD--TGKGHTQDQLDGVQRIKKCKTYYE 120

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           +LG+      ED++KAYRKL+LK HPDKN+APGA EAFK +  A+  LSN E RK+YDL 
Sbjct: 121 VLGVSTDAGEEDLKKAYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDLT 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAPR 240
           GSE+Q+  +   R+G   ++  +E D+  E++F  FF GG P+ + H  S N     +  
Sbjct: 181 GSEDQM--QNNHRNGGFDYHRGFEADITPEDLFNMFFGGGFPSGSVHTFS-NGRARYSHH 240

Query: 241 TGDHGSG---------GGLRTLIQLLPVLVILLLN----FMPSSEPIYSLSRSYPYEHRF 300
              H SG         GG    IQL+P++V++L++    FM S+ P     RS     R 
Sbjct: 241 QHHHHSGHDREDERADGGFSMFIQLMPIIVLILVSLLSQFMVSNPPYSLYPRSGQATKRV 300

Query: 301 TTQKGVNYYVKSKNFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRR 340
           T    + YYV SK+F+ ++      +  LE+ ++ +Y   +  NC  E Q+R
Sbjct: 301 TENLQIAYYV-SKDFQSEYSGILLQK--LEKNIEEDYVANVRNNCWRERQQR 335

BLAST of Lsi06G009570.1 vs. ExPASy Swiss-Prot
Match: Q9QYI4 (DnaJ homolog subfamily B member 12 OS=Mus musculus OX=10090 GN=Dnajb12 PE=1 SV=2)

HSP 1 Score: 183.7 bits (465), Expect = 3.6e-45
Identity = 132/385 (34.29%), Postives = 199/385 (51.69%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           M+ NKD+A +C+ I  +A+++    RAL+F+ KA+RL P   V  L+  L N      G 
Sbjct: 1   MESNKDEAERCISIALKAIQSNQPERALRFLEKAQRLYPTPRVSALIESL-NQKPQSTGD 60

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSAT-YTEEHVEIVRQIKKKKDYY 120
               T      +  +   E PS      A+G +    SA  YT E V  V+++K+ KDYY
Sbjct: 61  HPQPTDTTHTTTKKAGGTETPS------ANGEAGGGESAKGYTSEQVAAVKRVKQCKDYY 120

Query: 121 EILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDL 180
           EILG+ +S + ED++KAYRKL+LK HPDKN+APGA EAFKA+  A+  LSN E RK+YD 
Sbjct: 121 EILGVSRSASDEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQ 180

Query: 181 VGSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRS---FNFGPG 240
            G ++    R    HG   F+  +E D+  E++F  FF GG P++  H  S     +   
Sbjct: 181 FGDDKSQAARHGHSHG--DFHRGFEADISPEDLFNMFFGGGFPSSNVHVYSNGRMRYTYQ 240

Query: 241 MAPRTGDHGSGGGLRTLIQLLPVLVILLLNFMPS---SEPIYSLSRSYPYEH---RFTTQ 300
                 D+   GGL   +QL+P+L+++L++ +     S P YSLS      H   R T  
Sbjct: 241 QRQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSPRPSVGHIHKRVTDH 300

Query: 301 KGVNYYVKSKNFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRE---- 360
             V YYV +  F +++  +S     +E+ V+ +Y   L  NC  E Q+++    R     
Sbjct: 301 LNVAYYV-ADTFSEEYTGSSLK--TVERNVEDDYIANLRNNCWKEKQQKEGLLYRARYFG 360

BLAST of Lsi06G009570.1 vs. ExPASy Swiss-Prot
Match: Q58DR2 (DnaJ homolog subfamily B member 12 OS=Bos taurus OX=9913 GN=DNAJB12 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 3.1e-44
Identity = 128/369 (34.69%), Postives = 196/369 (53.12%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           M+ NKD+A +C+ I  +A+++    RAL+F+ KA+RL P   V  L+  L      Q  G
Sbjct: 1   MESNKDEAERCISIALKAIQSNQPDRALRFLEKAQRLYPTPRVHALIESLN--QKPQPAG 60

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           D        P  +   +  + +      A+G +   ++  YT E V  V+++K+ KDYYE
Sbjct: 61  D-----QPQPTEATHTTHRKAAGANTASANGEAGGESTKGYTAEQVAAVKRVKQCKDYYE 120

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILG+ +  + ED++KAYRKL+LK HPDKN+APGA EAFKA+  A+  LSN E RK+YD  
Sbjct: 121 ILGVSRGASDEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQF 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRS---FNFGPGM 240
           G ++    R    HG   F+  +E D+  E++F  FF GG P++  H  S     +    
Sbjct: 181 GDDKGQAARH--GHGHGDFHRGFEADISPEDLFNMFFGGGFPSSNVHVYSNGRMRYTYHQ 240

Query: 241 APRTGDHGSGGGLRTLIQLLPVLVILLLNFMPS---SEPIYSLSRSYPYEH---RFTTQK 300
                ++   GGL   +QL+P+L+++L++ +     S P YSLS      H   R T   
Sbjct: 241 RQDRRENQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSLRPSVGHVHKRVTDHL 300

Query: 301 GVNYYVKSKNFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFI---RETP 358
            V YYV +  F Q++  +S    ++E+ V+ +Y   L  NC  E Q+++ G +   R   
Sbjct: 301 NVVYYV-ADTFSQEYTGSSLK--MVERNVEDDYIANLRNNCWKEKQQKE-GLLYRARYFG 356

BLAST of Lsi06G009570.1 vs. ExPASy TrEMBL
Match: A0A1S3BA76 (chaperone protein dnaJ 49-like OS=Cucumis melo OX=3656 GN=LOC103487463 PE=4 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.0e-191
Identity = 338/360 (93.89%), Postives = 349/360 (96.94%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDD LKCLRIGKEALEAGNL RALKFVNKARRLDP L VDDLLP+LKNVSGDQLGG
Sbjct: 1   MDGNKDDGLKCLRIGKEALEAGNLTRALKFVNKARRLDPTLHVDDLLPKLKNVSGDQLGG 60

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE GNGS GSS SKSPEQPSVRRRVPASGSSESSTS TY EEHVEIVRQ+KKKKDYYE
Sbjct: 61  DACEAGNGSSGSSSSKSPEQPSVRRRVPASGSSESSTSTTYNEEHVEIVRQLKKKKDYYE 120

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDVV 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAPR 240
           GSEEQVYERRTARHGA+GFNGFYEGDVDAEEIFRNFFFGGMP TTTHFRSFNFGPG+APR
Sbjct: 181 GSEEQVYERRTARHGAHGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGVAPR 240

Query: 241 TGDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQKGVNYYVKSK 300
           TGDHGSGGGLRTLIQL+PVLVILLLNFMPSSEPIY+LSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 241 TGDHGSGGGLRTLIQLIPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 300

Query: 301 NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQSTA 360
           NFEQDFPP+SRDRLVLEQ+VDREYF+ILAQNCRLEVQRRQWGFIRETPHCDML+KFQS A
Sbjct: 301 NFEQDFPPSSRDRLVLEQKVDREYFDILAQNCRLEVQRRQWGFIRETPHCDMLDKFQSAA 360

BLAST of Lsi06G009570.1 vs. ExPASy TrEMBL
Match: A0A5A7TZX0 (Chaperone protein dnaJ 49-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00790 PE=4 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.0e-191
Identity = 338/360 (93.89%), Postives = 349/360 (96.94%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDD LKCLRIGKEALEAGNL RALKFVNKARRLDP L VDDLLP+LKNVSGDQLGG
Sbjct: 125 MDGNKDDGLKCLRIGKEALEAGNLTRALKFVNKARRLDPTLHVDDLLPKLKNVSGDQLGG 184

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE GNGS GSS SKSPEQPSVRRRVPASGSSESSTS TY EEHVEIVRQ+KKKKDYYE
Sbjct: 185 DACEAGNGSSGSSSSKSPEQPSVRRRVPASGSSESSTSTTYNEEHVEIVRQLKKKKDYYE 244

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 245 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDVV 304

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAPR 240
           GSEEQVYERRTARHGA+GFNGFYEGDVDAEEIFRNFFFGGMP TTTHFRSFNFGPG+APR
Sbjct: 305 GSEEQVYERRTARHGAHGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGVAPR 364

Query: 241 TGDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQKGVNYYVKSK 300
           TGDHGSGGGLRTLIQL+PVLVILLLNFMPSSEPIY+LSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 365 TGDHGSGGGLRTLIQLIPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 424

Query: 301 NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQSTA 360
           NFEQDFPP+SRDRLVLEQ+VDREYF+ILAQNCRLEVQRRQWGFIRETPHCDML+KFQS A
Sbjct: 425 NFEQDFPPSSRDRLVLEQKVDREYFDILAQNCRLEVQRRQWGFIRETPHCDMLDKFQSAA 484

BLAST of Lsi06G009570.1 vs. ExPASy TrEMBL
Match: A0A5D3DT80 (Chaperone protein dnaJ 49-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold876G00010 PE=4 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.0e-191
Identity = 338/360 (93.89%), Postives = 349/360 (96.94%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDD LKCLRIGKEALEAGNL RALKFVNKARRLDP L VDDLLP+LKNVSGDQLGG
Sbjct: 1   MDGNKDDGLKCLRIGKEALEAGNLTRALKFVNKARRLDPTLHVDDLLPKLKNVSGDQLGG 60

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE GNGS GSS SKSPEQPSVRRRVPASGSSESSTS TY EEHVEIVRQ+KKKKDYYE
Sbjct: 61  DACEAGNGSSGSSSSKSPEQPSVRRRVPASGSSESSTSTTYNEEHVEIVRQLKKKKDYYE 120

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDVV 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAPR 240
           GSEEQVYERRTARHGA+GFNGFYEGDVDAEEIFRNFFFGGMP TTTHFRSFNFGPG+APR
Sbjct: 181 GSEEQVYERRTARHGAHGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGVAPR 240

Query: 241 TGDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQKGVNYYVKSK 300
           TGDHGSGGGLRTLIQL+PVLVILLLNFMPSSEPIY+LSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 241 TGDHGSGGGLRTLIQLIPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 300

Query: 301 NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQSTA 360
           NFEQDFPP+SRDRLVLEQ+VDREYF+ILAQNCRLEVQRRQWGFIRETPHCDML+KFQS A
Sbjct: 301 NFEQDFPPSSRDRLVLEQKVDREYFDILAQNCRLEVQRRQWGFIRETPHCDMLDKFQSAA 360

BLAST of Lsi06G009570.1 vs. ExPASy TrEMBL
Match: A0A6J1JHS2 (chaperone protein dnaJ 49-like OS=Cucurbita maxima OX=3661 GN=LOC111485216 PE=4 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.3e-190
Identity = 338/361 (93.63%), Postives = 349/361 (96.68%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDP LPVDDLLPELKNVSGDQLGG
Sbjct: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60

Query: 61  DACETGNGSPGSSPSKSPE-QPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYY 120
           DAC+ G+G  GS  SKSPE QPSVRRRVPASGSSESSTSATYTEE+VEIVRQIKKKKDYY
Sbjct: 61  DACDAGHGPSGSPSSKSPEQQPSVRRRVPASGSSESSTSATYTEENVEIVRQIKKKKDYY 120

Query: 121 EILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDL 180
           EILGLEKSC+IEDVRK+YRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRK YDL
Sbjct: 121 EILGLEKSCSIEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKTYDL 180

Query: 181 VGSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAP 240
           VGSEE+VYERRTARHG NGFNGFYEGDVDA+EIFRNFFFGGMP TTTHFRSFNFGPGMAP
Sbjct: 181 VGSEEEVYERRTARHGGNGFNGFYEGDVDADEIFRNFFFGGMPPTTTHFRSFNFGPGMAP 240

Query: 241 RTGDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQKGVNYYVKS 300
           RTGD GSGGGLRTLIQLLPVLVILLLNFMPSSEPIY+LSRSYPYEHRFTTQKGVNYYVKS
Sbjct: 241 RTGDQGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKS 300

Query: 301 KNFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQST 360
           KNFEQ+FPPNSRDRLVL+QRVDREYFNILAQNCRLE+QRRQWGFIRETPHCDMLNKFQS 
Sbjct: 301 KNFEQEFPPNSRDRLVLDQRVDREYFNILAQNCRLEMQRRQWGFIRETPHCDMLNKFQSA 360

BLAST of Lsi06G009570.1 vs. ExPASy TrEMBL
Match: A0A6J1CTJ0 (chaperone protein dnaJ 49-like OS=Momordica charantia OX=3673 GN=LOC111014450 PE=4 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 9.5e-190
Identity = 337/360 (93.61%), Postives = 345/360 (95.83%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCL+IGKEALEAGNLVRALKFVNKARRLDPALP+DDLLPELKNVSG+QLG 
Sbjct: 1   MDGNKDDALKCLKIGKEALEAGNLVRALKFVNKARRLDPALPIDDLLPELKNVSGEQLGA 60

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE GNGS GS  SKS +QPS+RRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE
Sbjct: 61  DACEAGNGSSGSPSSKSSDQPSLRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGLEKSC+IEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 121 ILGLEKSCSIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDIV 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAPR 240
           GSEE VYERRTARHGANGFNGFYE DVDAEEIFRNFFFGGM  TTTHFRSFNFGPGM  R
Sbjct: 181 GSEEPVYERRTARHGANGFNGFYEADVDAEEIFRNFFFGGMAPTTTHFRSFNFGPGMGHR 240

Query: 241 TGDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQKGVNYYVKSK 300
           TGDHGSGG  RTLIQLLPVLVILLLNFMPSSEPIY+LSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 241 TGDHGSGGVFRTLIQLLPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 300

Query: 301 NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQSTA 360
           NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQS A
Sbjct: 301 NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQSAA 360

BLAST of Lsi06G009570.1 vs. NCBI nr
Match: XP_008444016.1 (PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016899821.1 PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >TYK26921.1 chaperone protein dnaJ 49-like [Cucumis melo var. makuwa])

HSP 1 Score: 679.1 bits (1751), Expect = 2.1e-191
Identity = 338/360 (93.89%), Postives = 349/360 (96.94%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDD LKCLRIGKEALEAGNL RALKFVNKARRLDP L VDDLLP+LKNVSGDQLGG
Sbjct: 1   MDGNKDDGLKCLRIGKEALEAGNLTRALKFVNKARRLDPTLHVDDLLPKLKNVSGDQLGG 60

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE GNGS GSS SKSPEQPSVRRRVPASGSSESSTS TY EEHVEIVRQ+KKKKDYYE
Sbjct: 61  DACEAGNGSSGSSSSKSPEQPSVRRRVPASGSSESSTSTTYNEEHVEIVRQLKKKKDYYE 120

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDVV 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAPR 240
           GSEEQVYERRTARHGA+GFNGFYEGDVDAEEIFRNFFFGGMP TTTHFRSFNFGPG+APR
Sbjct: 181 GSEEQVYERRTARHGAHGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGVAPR 240

Query: 241 TGDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQKGVNYYVKSK 300
           TGDHGSGGGLRTLIQL+PVLVILLLNFMPSSEPIY+LSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 241 TGDHGSGGGLRTLIQLIPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 300

Query: 301 NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQSTA 360
           NFEQDFPP+SRDRLVLEQ+VDREYF+ILAQNCRLEVQRRQWGFIRETPHCDML+KFQS A
Sbjct: 301 NFEQDFPPSSRDRLVLEQKVDREYFDILAQNCRLEVQRRQWGFIRETPHCDMLDKFQSAA 360

BLAST of Lsi06G009570.1 vs. NCBI nr
Match: KAA0046789.1 (chaperone protein dnaJ 49-like [Cucumis melo var. makuwa])

HSP 1 Score: 679.1 bits (1751), Expect = 2.1e-191
Identity = 338/360 (93.89%), Postives = 349/360 (96.94%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDD LKCLRIGKEALEAGNL RALKFVNKARRLDP L VDDLLP+LKNVSGDQLGG
Sbjct: 125 MDGNKDDGLKCLRIGKEALEAGNLTRALKFVNKARRLDPTLHVDDLLPKLKNVSGDQLGG 184

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE GNGS GSS SKSPEQPSVRRRVPASGSSESSTS TY EEHVEIVRQ+KKKKDYYE
Sbjct: 185 DACEAGNGSSGSSSSKSPEQPSVRRRVPASGSSESSTSTTYNEEHVEIVRQLKKKKDYYE 244

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 245 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDVV 304

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAPR 240
           GSEEQVYERRTARHGA+GFNGFYEGDVDAEEIFRNFFFGGMP TTTHFRSFNFGPG+APR
Sbjct: 305 GSEEQVYERRTARHGAHGFNGFYEGDVDAEEIFRNFFFGGMPPTTTHFRSFNFGPGVAPR 364

Query: 241 TGDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQKGVNYYVKSK 300
           TGDHGSGGGLRTLIQL+PVLVILLLNFMPSSEPIY+LSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 365 TGDHGSGGGLRTLIQLIPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 424

Query: 301 NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQSTA 360
           NFEQDFPP+SRDRLVLEQ+VDREYF+ILAQNCRLEVQRRQWGFIRETPHCDML+KFQS A
Sbjct: 425 NFEQDFPPSSRDRLVLEQKVDREYFDILAQNCRLEVQRRQWGFIRETPHCDMLDKFQSAA 484

BLAST of Lsi06G009570.1 vs. NCBI nr
Match: XP_022987765.1 (chaperone protein dnaJ 49-like [Cucurbita maxima])

HSP 1 Score: 673.7 bits (1737), Expect = 8.8e-190
Identity = 338/361 (93.63%), Postives = 349/361 (96.68%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDP LPVDDLLPELKNVSGDQLGG
Sbjct: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60

Query: 61  DACETGNGSPGSSPSKSPE-QPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYY 120
           DAC+ G+G  GS  SKSPE QPSVRRRVPASGSSESSTSATYTEE+VEIVRQIKKKKDYY
Sbjct: 61  DACDAGHGPSGSPSSKSPEQQPSVRRRVPASGSSESSTSATYTEENVEIVRQIKKKKDYY 120

Query: 121 EILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDL 180
           EILGLEKSC+IEDVRK+YRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRK YDL
Sbjct: 121 EILGLEKSCSIEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKTYDL 180

Query: 181 VGSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAP 240
           VGSEE+VYERRTARHG NGFNGFYEGDVDA+EIFRNFFFGGMP TTTHFRSFNFGPGMAP
Sbjct: 181 VGSEEEVYERRTARHGGNGFNGFYEGDVDADEIFRNFFFGGMPPTTTHFRSFNFGPGMAP 240

Query: 241 RTGDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQKGVNYYVKS 300
           RTGD GSGGGLRTLIQLLPVLVILLLNFMPSSEPIY+LSRSYPYEHRFTTQKGVNYYVKS
Sbjct: 241 RTGDQGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKS 300

Query: 301 KNFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQST 360
           KNFEQ+FPPNSRDRLVL+QRVDREYFNILAQNCRLE+QRRQWGFIRETPHCDMLNKFQS 
Sbjct: 301 KNFEQEFPPNSRDRLVLDQRVDREYFNILAQNCRLEMQRRQWGFIRETPHCDMLNKFQSA 360

BLAST of Lsi06G009570.1 vs. NCBI nr
Match: XP_022144873.1 (chaperone protein dnaJ 49-like [Momordica charantia] >XP_022144874.1 chaperone protein dnaJ 49-like [Momordica charantia] >XP_022144875.1 chaperone protein dnaJ 49-like [Momordica charantia])

HSP 1 Score: 672.5 bits (1734), Expect = 2.0e-189
Identity = 337/360 (93.61%), Postives = 345/360 (95.83%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCL+IGKEALEAGNLVRALKFVNKARRLDPALP+DDLLPELKNVSG+QLG 
Sbjct: 1   MDGNKDDALKCLKIGKEALEAGNLVRALKFVNKARRLDPALPIDDLLPELKNVSGEQLGA 60

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
           DACE GNGS GS  SKS +QPS+RRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE
Sbjct: 61  DACEAGNGSSGSPSSKSSDQPSLRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGLEKSC+IEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYD+V
Sbjct: 121 ILGLEKSCSIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDIV 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAPR 240
           GSEE VYERRTARHGANGFNGFYE DVDAEEIFRNFFFGGM  TTTHFRSFNFGPGM  R
Sbjct: 181 GSEEPVYERRTARHGANGFNGFYEADVDAEEIFRNFFFGGMAPTTTHFRSFNFGPGMGHR 240

Query: 241 TGDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQKGVNYYVKSK 300
           TGDHGSGG  RTLIQLLPVLVILLLNFMPSSEPIY+LSRSYPYEHRFTTQKGVNYYVKSK
Sbjct: 241 TGDHGSGGVFRTLIQLLPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKSK 300

Query: 301 NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQSTA 360
           NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQS A
Sbjct: 301 NFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQSAA 360

BLAST of Lsi06G009570.1 vs. NCBI nr
Match: XP_023515966.1 (chaperone protein dnaJ 49-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 667.9 bits (1722), Expect = 4.8e-188
Identity = 335/361 (92.80%), Postives = 346/361 (95.84%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDDA+KCLRIGKEALEAGNLVRALKFVNKARRLDP LPVDDLLPELKNVSGDQLGG
Sbjct: 1   MDGNKDDAVKCLRIGKEALEAGNLVRALKFVNKARRLDPTLPVDDLLPELKNVSGDQLGG 60

Query: 61  DACETGNGSPGSSPSKSPE-QPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYY 120
           DAC+ GNG  G   SKSPE QPSVRRRVPASGSSESS SA YTEE+VEIVRQIKKKKDYY
Sbjct: 61  DACDAGNGPSGFPSSKSPEQQPSVRRRVPASGSSESSMSAAYTEENVEIVRQIKKKKDYY 120

Query: 121 EILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDL 180
           EILGLEKSC+IEDVRK+YRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRK YDL
Sbjct: 121 EILGLEKSCSIEDVRKSYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKTYDL 180

Query: 181 VGSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPGMAP 240
           VGSEE+VYERRTARHG NGFNGFYEGDVDA+EIFRNFFFGGMP TTTHFRSFNFGPGMAP
Sbjct: 181 VGSEEEVYERRTARHGGNGFNGFYEGDVDADEIFRNFFFGGMPPTTTHFRSFNFGPGMAP 240

Query: 241 RTGDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQKGVNYYVKS 300
           RTGD GSGGGLRTLIQLLPVLVILLLNFMPSSEPIY+LSRSYPYEHRFTTQKGVNYYVKS
Sbjct: 241 RTGDQGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYALSRSYPYEHRFTTQKGVNYYVKS 300

Query: 301 KNFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPHCDMLNKFQST 360
           KNFEQ+FPPNSRDRLVL+QRVDREYFNILAQNCRLE+QRRQWGFIRETPHCDMLNKFQS 
Sbjct: 301 KNFEQEFPPNSRDRLVLDQRVDREYFNILAQNCRLEMQRRQWGFIRETPHCDMLNKFQSA 360

BLAST of Lsi06G009570.1 vs. TAIR 10
Match: AT3G57340.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 424.1 bits (1089), Expect = 1.1e-118
Identity = 225/377 (59.68%), Postives = 288/377 (76.39%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCL+I K A+EAG+  RALKF+ KARRLDP LP+DDL+ EL N   D+ G 
Sbjct: 1   MDGNKDDALKCLKICKSAMEAGDRPRALKFLAKARRLDPNLPIDDLVSELNNNKSDEPGS 60

Query: 61  DACETGNGSPGSSPSK----SPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKK 120
                   SPGS+ +K    S ++PS+R+R   S ++ SS+S +YTEE + IVR+IK KK
Sbjct: 61  AK------SPGSAAAKDSSNSSDRPSLRQR--GSSTTSSSSSMSYTEEQISIVRKIKSKK 120

Query: 121 DYYEILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKR 180
           DYYEILGLE +C+++DVRKAYRKLSLKVHPDKN APG+EEAFK+VSKAFQCLSN+E+RK+
Sbjct: 121 DYYEILGLESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKK 180

Query: 181 YDLVGSEEQVYE-RRTARHGANGFNG--FYEGDVDAEEIFRNFF------FGGMPATTTH 240
           YD+ GS+E +Y+ RR+AR  +NGFNG  +YE + D  EIFR+FF       GGMP  T  
Sbjct: 181 YDVSGSDEPIYQPRRSAR--SNGFNGGYYYEDEFDPNEIFRSFFGGGGFGGGGMPPATAQ 240

Query: 241 FRSFNFGPGMAPRTGDHG----SGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPY 300
           FRSFNFG     RT ++     +G   R L+QLLPV+ ILLLNFMPSS+P+Y LS +YPY
Sbjct: 241 FRSFNFG-ATRQRTANNNQAPDAGFNARILLQLLPVVFILLLNFMPSSQPVYQLSATYPY 300

Query: 301 EHRFTTQKGVNYYVKSKNFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGF 360
           +++FTTQKGVNY+VKS  FEQD+P +S DR  LE++V+R+Y +IL+QNCR E+QR+QWGF
Sbjct: 301 QYKFTTQKGVNYFVKSSKFEQDYPRDSNDRHTLEEQVERDYVSILSQNCRYEMQRKQWGF 360

BLAST of Lsi06G009570.1 vs. TAIR 10
Match: AT3G57340.2 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 424.1 bits (1089), Expect = 1.1e-118
Identity = 225/377 (59.68%), Postives = 288/377 (76.39%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCL+I K A+EAG+  RALKF+ KARRLDP LP+DDL+ EL N   D+ G 
Sbjct: 1   MDGNKDDALKCLKICKSAMEAGDRPRALKFLAKARRLDPNLPIDDLVSELNNNKSDEPGS 60

Query: 61  DACETGNGSPGSSPSK----SPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKK 120
                   SPGS+ +K    S ++PS+R+R   S ++ SS+S +YTEE + IVR+IK KK
Sbjct: 61  AK------SPGSAAAKDSSNSSDRPSLRQR--GSSTTSSSSSMSYTEEQISIVRKIKSKK 120

Query: 121 DYYEILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKR 180
           DYYEILGLE +C+++DVRKAYRKLSLKVHPDKN APG+EEAFK+VSKAFQCLSN+E+RK+
Sbjct: 121 DYYEILGLESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSKAFQCLSNDEARKK 180

Query: 181 YDLVGSEEQVYE-RRTARHGANGFNG--FYEGDVDAEEIFRNFF------FGGMPATTTH 240
           YD+ GS+E +Y+ RR+AR  +NGFNG  +YE + D  EIFR+FF       GGMP  T  
Sbjct: 181 YDVSGSDEPIYQPRRSAR--SNGFNGGYYYEDEFDPNEIFRSFFGGGGFGGGGMPPATAQ 240

Query: 241 FRSFNFGPGMAPRTGDHG----SGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPY 300
           FRSFNFG     RT ++     +G   R L+QLLPV+ ILLLNFMPSS+P+Y LS +YPY
Sbjct: 241 FRSFNFG-ATRQRTANNNQAPDAGFNARILLQLLPVVFILLLNFMPSSQPVYQLSATYPY 300

Query: 301 EHRFTTQKGVNYYVKSKNFEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGF 360
           +++FTTQKGVNY+VKS  FEQD+P +S DR  LE++V+R+Y +IL+QNCR E+QR+QWGF
Sbjct: 301 QYKFTTQKGVNYFVKSSKFEQDYPRDSNDRHTLEEQVERDYVSILSQNCRYEMQRKQWGF 360

BLAST of Lsi06G009570.1 vs. TAIR 10
Match: AT5G05750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 308.1 bits (788), Expect = 9.0e-84
Identity = 168/290 (57.93%), Postives = 213/290 (73.45%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDDALKCL+IGK+A+EAG+  RALKF+ KARRLDP LP+D L+ +LK  S +    
Sbjct: 1   MDGNKDDALKCLKIGKDAIEAGDRSRALKFLEKARRLDPNLPIDGLVSDLKKQSDEPA-- 60

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
            A E   GS  +  SK  ++PS+R+R  +S ++ SS+S++ TEE   IVR+IK KKDYYE
Sbjct: 61  -AEEDSPGSAANESSKPSDRPSLRQRGSSSSAAGSSSSSSSTEEQRTIVREIKSKKDYYE 120

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGL+ +C++ED+RK+YRKLSLKVHPDKN APG+EEAFK+VSKAFQCLSNE++R++YD  
Sbjct: 121 ILGLKSNCSVEDLRKSYRKLSLKVHPDKNKAPGSEEAFKSVSKAFQCLSNEDTRRKYDGS 180

Query: 181 GSEEQVYERRTARHGANGFNGFYEGDVDAEEIFRNFFFGG-MPATTTHFRSFNFGPGMAP 240
           GS+E  Y+ R      NGFNGFY+ + DA+EIFR+FF GG M   TT FRSFNFG G   
Sbjct: 181 GSDEPAYQPRRDARRNNGFNGFYDDEFDADEIFRSFFGGGEMNPATTQFRSFNFGGGTRT 240

Query: 241 RTGDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTT 290
                 +G   R L+Q+LPV+ ILLLNF+PS +PIYSLS      HR TT
Sbjct: 241 ANQASDTGFNPRVLLQILPVVFILLLNFLPSPQPIYSLS------HRITT 281

BLAST of Lsi06G009570.1 vs. TAIR 10
Match: AT5G49060.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 240.0 bits (611), Expect = 3.0e-63
Identity = 148/361 (41.00%), Postives = 214/361 (59.28%), Query Frame = 0

Query: 1   MDGNKDDALKCLRIGKEALEAGNLVRALKFVNKARRLDPALPVDDLLPELKNVSGDQLGG 60
           MDGNKDDA +CLRI ++A+ +G+  RALKF+N A+RL+P+L VD+L+    N+       
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVAACDNL------- 60

Query: 61  DACETGNGSPGSSPSKSPEQPSVRRRVPASGSSESSTSATYTEEHVEIVRQIKKKKDYYE 120
                       S S++       + +        +    YTEE+V++VR I +  DYY 
Sbjct: 61  -----------DSVSRNSSVSEKLKTMDGDDDKLETGKMKYTEENVDLVRNIIRNNDYYA 120

Query: 121 ILGLEKSCTIEDVRKAYRKLSLKVHPDKNNAPGAEEAFKAVSKAFQCLSNEESRKRYDLV 180
           ILGLEK+C+++++RKAYRKLSLKVHPDKN APG+EEAFK VSKAF CLS+  SR+++D V
Sbjct: 121 ILGLEKNCSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQV 180

Query: 181 GSEEQV----YERRTARHGANGFNGFYEGDVDAEEIFRNFFFGGMPATTTHFRSFNFGPG 240
           G  ++        R  R   N  N F++ + D EEIFR  F  G       FR+ +    
Sbjct: 181 GIVDEFDHVQRRNRRPRRRYNTRNDFFDDEFDPEEIFRTVF--GQQREV--FRASHAYRT 240

Query: 241 MAPRT-----GDHGSGGGLRTLIQLLPVLVILLLNFMPSSEPIYSLSRSYPYEHRFTTQK 300
             PR        + +G    T+IQ+LP  ++LLL ++P SEP YSL ++  Y+   TTQ 
Sbjct: 241 RQPRNQFREEEINVAGPSCLTIIQILPFFLLLLLAYLPFSEPDYSLHKNQSYQIPKTTQN 300

Query: 301 -GVNYYVKSKN-FEQDFPPNSRDRLVLEQRVDREYFNILAQNCRLEVQRRQWGFIRETPH 351
             +++YV+S + F++ FP +S  R  LE  V +EY + L Q+CR+E+Q+R+W     TPH
Sbjct: 301 TEISFYVRSASAFDEKFPLSSSARANLEGNVIKEYKHFLFQSCRIELQKRRWNKKIPTPH 339

BLAST of Lsi06G009570.1 vs. TAIR 10
Match: AT2G20560.1 (DNAJ heat shock family protein )

HSP 1 Score: 78.2 bits (191), Expect = 1.5e-14
Identity = 55/149 (36.91%), Postives = 79/149 (53.02%), Query Frame = 0

Query: 117 DYYEILGLEKSCTIEDVRKAYRKLSLKVHPDK--NNAPGAEEAFKAVSKAFQCLSNEESR 176
           DYY++L +++S + +D++KAYRKL++K HPDK  NN   AE  FK +S+A++ LS+ + +
Sbjct: 4   DYYKVLQVDRSASDDDLKKAYRKLAMKWHPDKNPNNKKDAEAMFKQISEAYEVLSDPQKK 63

Query: 177 KRYDLVGSEEQVYERRTARHGANGFNGFYEGD---------VDAEEIFRNFF-----FGG 236
             YD  G  E+  +       A G   F  GD          +A++IF  FF     FGG
Sbjct: 64  AVYDQYG--EEGLKGNVPPPDAGGATYFSTGDGPTSFRFNPRNADDIFAEFFGFSSPFGG 123

Query: 237 MPATTTHFRSFNFGPGMAPRTGDHGSGGG 250
                T F S  FG  M    G+ G GGG
Sbjct: 124 -GRGGTRFSSSMFGDNMFASFGEGGGGGG 149

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FH284.3e-6241.00Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2[more]
Q7ZXQ81.3e-4737.68DnaJ homolog subfamily B member 14 OS=Xenopus laevis OX=8355 GN=dnajb14 PE=2 SV=... [more]
Q28I385.0e-4736.36DnaJ homolog subfamily B member 14 OS=Xenopus tropicalis OX=8364 GN=dnajb14 PE=2... [more]
Q9QYI43.6e-4534.29DnaJ homolog subfamily B member 12 OS=Mus musculus OX=10090 GN=Dnajb12 PE=1 SV=2[more]
Q58DR23.1e-4434.69DnaJ homolog subfamily B member 12 OS=Bos taurus OX=9913 GN=DNAJB12 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BA761.0e-19193.89chaperone protein dnaJ 49-like OS=Cucumis melo OX=3656 GN=LOC103487463 PE=4 SV=1[more]
A0A5A7TZX01.0e-19193.89Chaperone protein dnaJ 49-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A5D3DT801.0e-19193.89Chaperone protein dnaJ 49-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A6J1JHS24.3e-19093.63chaperone protein dnaJ 49-like OS=Cucurbita maxima OX=3661 GN=LOC111485216 PE=4 ... [more]
A0A6J1CTJ09.5e-19093.61chaperone protein dnaJ 49-like OS=Momordica charantia OX=3673 GN=LOC111014450 PE... [more]
Match NameE-valueIdentityDescription
XP_008444016.12.1e-19193.89PREDICTED: chaperone protein dnaJ 49-like [Cucumis melo] >XP_016899821.1 PREDICT... [more]
KAA0046789.12.1e-19193.89chaperone protein dnaJ 49-like [Cucumis melo var. makuwa][more]
XP_022987765.18.8e-19093.63chaperone protein dnaJ 49-like [Cucurbita maxima][more]
XP_022144873.12.0e-18993.61chaperone protein dnaJ 49-like [Momordica charantia] >XP_022144874.1 chaperone p... [more]
XP_023515966.14.8e-18892.80chaperone protein dnaJ 49-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G57340.11.1e-11859.68Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT3G57340.21.1e-11859.68Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT5G05750.19.0e-8457.93DNAJ heat shock N-terminal domain-containing protein [more]
AT5G49060.13.0e-6341.00Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT2G20560.11.5e-1436.91DNAJ heat shock family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 153..173
score: 37.2
coord: 137..152
score: 53.36
coord: 119..137
score: 40.17
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 116..173
e-value: 4.8E-26
score: 102.5
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 117..178
e-value: 1.6E-23
score: 82.5
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 117..181
score: 20.11388
IPR001623DnaJ domainCDDcd06257DnaJcoord: 117..170
e-value: 2.57055E-21
score: 84.1341
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 113..228
e-value: 3.5E-31
score: 110.0
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 112..227
IPR015399Domain of unknown function DUF1977, DnaJ-likePFAMPF09320DUF1977coord: 270..340
e-value: 1.3E-9
score: 38.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 54..102
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 66..102
NoneNo IPR availablePANTHERPTHR43908AT29763P-RELATEDcoord: 1..360
NoneNo IPR availablePANTHERPTHR43908:SF9SUBFAMILY NOT NAMEDcoord: 1..360
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 158..177

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi06G009570Lsi06G009570gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi06G009570.1.exon.1Lsi06G009570.1.exon.1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi06G009570.1.CDS.1Lsi06G009570.1.CDS.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi06G009570.1Lsi06G009570.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071218 cellular response to misfolded protein
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0030433 ubiquitin-dependent ERAD pathway
cellular_component GO:0005789 endoplasmic reticulum membrane
molecular_function GO:0030544 Hsp70 protein binding