Lsi04G003760.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G003760.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionHeparanase, putative
Locationchr04: 3721948 .. 3722756 (+)
Sequence length552
RNA-Seq ExpressionLsi04G003760.1
SyntenyLsi04G003760.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCATTAATTTAATTTAATTGTATTTATGTTTTTGATTGATTAAGGTATTTGGATCAATTGGGGATGACTGCCACATTCAATCATCAGGTTTATTGCAGACAAGCCTTGATTGGTGGAAACTATGCTTTGCTTAATACCACTACTTTCATCCCTAACCCTGATTATTATAGGTCAGTAATTCTAATTAATTTTTTTAAAAAAAAATTGAAATATCCCAATAATTATATTCAGTAACTAAATATTGTTTGGTTGGAAACAGCGCTCTGTTATGGCACAGATTAATGGGAAAGAAGGTCCTCTCAGCATCCCACGACAGCTCTCCATACCTTCGTGTTTATTCTCACTGCACCAAAAACAACGTAATTAACTCAAAAACATTTCTCTTATATTAATAATTAAAAAAAAAAACATGTTTTTTTTTATTGTATTTAATTTTATACTAATGTTTATTATTATTATTATTATTATTGAATTGTTTTCAGGAAGGCGTTACATTGCTTCTAATTAACATGTCAAATTCGACAACATTTGATGTAAATATTGTTAACGATAGAAATATTTATCCAAATTTCGTAAGCAGCAGCAAATTAAAAGCAAGAGAAGAATACCATTTAACTCCTAAAGACGGCAATATTCAAAGCGACGTTATGCTTTTAAATGGAACTCCTCTTCAACTTACTCCAACGTTGGACATTCCCGACATGTCGCCCAAGCTCGTCGATCCCACTTCCGGTGTGTCGGTTGCCCCTGATTCTTACGTCTTTGTCTCCATCAAAGACTTCAAAGCTTCTGCTTGCGCTTAA

mRNA sequence

ATGGAGTATTTGGATCAATTGGGGATGACTGCCACATTCAATCATCAGGTTTATTGCAGACAAGCCTTGATTGGTGGAAACTATGCTTTGCTTAATACCACTACTTTCATCCCTAACCCTGATTATTATAGCGCTCTGTTATGGCACAGATTAATGGGAAAGAAGGTCCTCTCAGCATCCCACGACAGCTCTCCATACCTTCGTGTTTATTCTCACTGCACCAAAAACAACGAAGGCGTTACATTGCTTCTAATTAACATGTCAAATTCGACAACATTTGATGTAAATATTGTTAACGATAGAAATATTTATCCAAATTTCGTAAGCAGCAGCAAATTAAAAGCAAGAGAAGAATACCATTTAACTCCTAAAGACGGCAATATTCAAAGCGACGTTATGCTTTTAAATGGAACTCCTCTTCAACTTACTCCAACGTTGGACATTCCCGACATGTCGCCCAAGCTCGTCGATCCCACTTCCGGTGTGTCGGTTGCCCCTGATTCTTACGTCTTTGTCTCCATCAAAGACTTCAAAGCTTCTGCTTGCGCTTAA

Coding sequence (CDS)

ATGGAGTATTTGGATCAATTGGGGATGACTGCCACATTCAATCATCAGGTTTATTGCAGACAAGCCTTGATTGGTGGAAACTATGCTTTGCTTAATACCACTACTTTCATCCCTAACCCTGATTATTATAGCGCTCTGTTATGGCACAGATTAATGGGAAAGAAGGTCCTCTCAGCATCCCACGACAGCTCTCCATACCTTCGTGTTTATTCTCACTGCACCAAAAACAACGAAGGCGTTACATTGCTTCTAATTAACATGTCAAATTCGACAACATTTGATGTAAATATTGTTAACGATAGAAATATTTATCCAAATTTCGTAAGCAGCAGCAAATTAAAAGCAAGAGAAGAATACCATTTAACTCCTAAAGACGGCAATATTCAAAGCGACGTTATGCTTTTAAATGGAACTCCTCTTCAACTTACTCCAACGTTGGACATTCCCGACATGTCGCCCAAGCTCGTCGATCCCACTTCCGGTGTGTCGGTTGCCCCTGATTCTTACGTCTTTGTCTCCATCAAAGACTTCAAAGCTTCTGCTTGCGCTTAA

Protein sequence

MEYLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHDSSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSKLKAREEYHLTPKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYVFVSIKDFKASACA
Homology
BLAST of Lsi04G003760.1 vs. ExPASy Swiss-Prot
Match: Q9FF10 (Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 8.3e-46
Identity = 101/202 (50.00%), Postives = 126/202 (62.38%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLDQLGM+A  N +VYCRQ L+GG Y LL   TF+PNPDYYSALLWHRLMGK VL+   D
Sbjct: 342 YLDQLGMSARHNTKVYCRQTLVGGFYGLLEKGTFVPNPDYYSALLWHRLMGKGVLAVQTD 401

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSK------LKA- 122
             P LRVY+HC+K   GVTLLLIN+SN + F V++ N  N+  N  S  K      LK  
Sbjct: 402 GPPQLRVYAHCSKGRAGVTLLLINLSNQSDFTVSVSNGINVVLNAESRKKKSLLDTLKRP 461

Query: 123 --------------REEYHLTPKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSG 182
                         REEYHLTP++G ++S  M+LNG  L+ T T DIP + P L    S 
Sbjct: 462 FSWIGSKASDGYLNREEYHLTPENGVLRSKTMVLNGKSLKPTATGDIPSLEPVLRSVNSP 521

Query: 183 VSVAPDSYVFVSIKDFKASACA 184
           ++V P S  F+ + +F ASAC+
Sbjct: 522 LNVLPLSMSFIVLPNFDASACS 543

BLAST of Lsi04G003760.1 vs. ExPASy Swiss-Prot
Match: Q8L608 (Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.7e-43
Identity = 95/202 (47.03%), Postives = 122/202 (60.40%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLDQLG+++  N +VYCRQAL+GG Y LL   TF+PNPDYYSALLWHRLMGK +L     
Sbjct: 338 YLDQLGISSKHNTKVYCRQALVGGFYGLLEKETFVPNPDYYSALLWHRLMGKGILGVQTT 397

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIY--------PNFVSSSKLK 122
           +S YLR Y HC+K   G+T+LLIN+S  TTF V + N   +          +F+ + K K
Sbjct: 398 ASEYLRAYVHCSKRRAGITILLINLSKHTTFTVAVSNGVKVVLQAESMKRKSFLETIKSK 457

Query: 123 A-------------REEYHLTPKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSG 182
                         REEYHL+PKDG+++S +MLLNG PL  T T DIP + P      S 
Sbjct: 458 VSWVGNKASDGYLNREEYHLSPKDGDLRSKIMLLNGKPLVPTATGDIPKLEPVRHGVKSP 517

Query: 183 VSVAPDSYVFVSIKDFKASACA 184
           V + P S  F+ +  F A AC+
Sbjct: 518 VYINPLSISFIVLPTFDAPACS 539

BLAST of Lsi04G003760.1 vs. ExPASy Swiss-Prot
Match: Q9FZP1 (Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2)

HSP 1 Score: 169.5 bits (428), Expect = 3.6e-41
Identity = 85/196 (43.37%), Postives = 120/196 (61.22%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLDQLGM + ++ + YCRQ+LIGGNY LLNTT F PNPDYYSAL+W +LMG+K L  +  
Sbjct: 341 YLDQLGMASLYDTKTYCRQSLIGGNYGLLNTTNFTPNPDYYSALIWRQLMGRKALFTTFS 400

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTT----------FDVNIVNDRNIYPN-----F 122
            +  +R Y+HC + ++G+T+LL+N+ N+TT          F +        Y       F
Sbjct: 401 GTKKIRSYTHCARQSKGITVLLMNLDNTTTVVAKVELNNSFSLRHTKHMKSYKRASSQLF 460

Query: 123 VSSSKLKAREEYHLTPKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPD 182
              + +  REEYHLT KDGN+ S  MLLNG  LQ+    D+P + P  ++ T  +++AP 
Sbjct: 461 GGPNGVIQREEYHLTAKDGNLHSQTMLLNGNALQVNSMGDLPPIEPIHINSTEPITIAPY 520

Query: 183 SYVFVSIKDFKASACA 184
           S VFV +++    ACA
Sbjct: 521 SIVFVHMRNVVVPACA 536

BLAST of Lsi04G003760.1 vs. ExPASy Swiss-Prot
Match: Q9LRC8 (Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.2e-33
Identity = 74/172 (43.02%), Postives = 108/172 (62.79%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YL+ LG +A  + + +CRQ L GGNY LL T T+IPNPDYYSALLWHRLMG KVL     
Sbjct: 351 YLNMLGYSALLDTKTFCRQTLTGGNYGLLQTGTYIPNPDYYSALLWHRLMGSKVLKTEIV 410

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSKLKAREEYHLT 122
            +  + +Y+HC K + G+T+L++N    ++  +++  D + Y +         REEYHLT
Sbjct: 411 GTKNVYIYAHCAKKSNGITMLVLNHDGESSVKISL--DPSKYGS--------KREEYHLT 470

Query: 123 PKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYVFVSI 175
           P + N+QS ++ LNG  L L P+  IP ++P   D +  + VAP S++FV +
Sbjct: 471 PVNNNLQSRLVKLNGELLHLDPSGVIPALNPVEKDNSKQLEVAPYSFMFVHL 512

BLAST of Lsi04G003760.1 vs. ExPASy Swiss-Prot
Match: Q9MYY0 (Heparanase OS=Bos taurus OX=9913 GN=HPSE PE=2 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 1.4e-13
Identity = 65/192 (33.85%), Postives = 102/192 (53.12%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIG-GNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSAS- 62
           +LD+LG++A    +V  RQ L G GNY L++   F P PDY+ +LL+ +L+G KVL AS 
Sbjct: 367 WLDKLGLSARMGIEVVMRQVLFGAGNYHLVD-GNFEPLPDYWLSLLFKKLVGNKVLMASV 426

Query: 63  --HDSSPYLRVYSHCT-----KNNEG-VTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSK 122
              D S + RVY HCT     +  EG +TL  +N+ N        V      P+ + + +
Sbjct: 427 KGPDRSKF-RVYLHCTNTKHPRYKEGDLTLYALNLHN--------VTKHLELPHHLFNKQ 486

Query: 123 LKAREEYHLTPK--DGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYV 182
           +   ++Y + P   DG +   V  LNG  L++     +P ++ K + P S + + P SY 
Sbjct: 487 V---DKYLIKPSGTDGLLSKSVQ-LNGQILKMVDEQTLPALTEKPLHPGSSLGMPPFSYG 544

BLAST of Lsi04G003760.1 vs. ExPASy TrEMBL
Match: A0A5A7SZC5 (Heparanase-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G004670 PE=3 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 5.3e-88
Identity = 165/181 (91.16%), Postives = 172/181 (95.03%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLD LGMTA+F+HQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVL+ASHD
Sbjct: 398 YLDNLGMTASFDHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLAASHD 457

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSKLKAREEYHLT 122
           SSPYLRVYSHCTKN EGVT+LLIN+SNSTT DV IVNDRNIYPNFV  SKLKAREEYHLT
Sbjct: 458 SSPYLRVYSHCTKNEEGVTVLLINLSNSTTHDVKIVNDRNIYPNFV-GSKLKAREEYHLT 517

Query: 123 PKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYVFVSIKDFKASAC 182
           PKDG+IQSDVMLLNGTPLQLTPTLDIP MSPKLVDPTS V+VAP SYVFVSIKDFKASAC
Sbjct: 518 PKDGDIQSDVMLLNGTPLQLTPTLDIPKMSPKLVDPTSVVTVAPSSYVFVSIKDFKASAC 577

Query: 183 A 184
           A
Sbjct: 578 A 577

BLAST of Lsi04G003760.1 vs. ExPASy TrEMBL
Match: A0A5D3CBY1 (Heparanase-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00010 PE=3 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 5.3e-88
Identity = 165/181 (91.16%), Postives = 172/181 (95.03%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLD LGMTA+F+HQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVL+ASHD
Sbjct: 209 YLDNLGMTASFDHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLAASHD 268

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSKLKAREEYHLT 122
           SSPYLRVYSHCTKN EGVT+LLIN+SNSTT DV IVNDRNIYPNFV  SKLKAREEYHLT
Sbjct: 269 SSPYLRVYSHCTKNEEGVTVLLINLSNSTTHDVKIVNDRNIYPNFV-GSKLKAREEYHLT 328

Query: 123 PKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYVFVSIKDFKASAC 182
           PKDG+IQSDVMLLNGTPLQLTPTLDIP MSPKLVDPTS V+VAP SYVFVSIKDFKASAC
Sbjct: 329 PKDGDIQSDVMLLNGTPLQLTPTLDIPKMSPKLVDPTSVVTVAPSSYVFVSIKDFKASAC 388

Query: 183 A 184
           A
Sbjct: 389 A 388

BLAST of Lsi04G003760.1 vs. ExPASy TrEMBL
Match: A0A1S3BEI8 (heparanase-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103489169 PE=3 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 5.3e-88
Identity = 165/181 (91.16%), Postives = 172/181 (95.03%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLD LGMTA+F+HQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVL+ASHD
Sbjct: 338 YLDNLGMTASFDHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLAASHD 397

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSKLKAREEYHLT 122
           SSPYLRVYSHCTKN EGVT+LLIN+SNSTT DV IVNDRNIYPNFV  SKLKAREEYHLT
Sbjct: 398 SSPYLRVYSHCTKNEEGVTVLLINLSNSTTHDVKIVNDRNIYPNFV-GSKLKAREEYHLT 457

Query: 123 PKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYVFVSIKDFKASAC 182
           PKDG+IQSDVMLLNGTPLQLTPTLDIP MSPKLVDPTS V+VAP SYVFVSIKDFKASAC
Sbjct: 458 PKDGDIQSDVMLLNGTPLQLTPTLDIPKMSPKLVDPTSVVTVAPSSYVFVSIKDFKASAC 517

Query: 183 A 184
           A
Sbjct: 518 A 517

BLAST of Lsi04G003760.1 vs. ExPASy TrEMBL
Match: A0A0A0KQS3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G606560 PE=3 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 2.9e-86
Identity = 161/181 (88.95%), Postives = 170/181 (93.92%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLD LGMTA+F+HQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVL+ASHD
Sbjct: 338 YLDNLGMTASFDHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLAASHD 397

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSKLKAREEYHLT 122
           SSPYLRVYSHCT+N+EGVT+LLIN+SNSTT DV IVNDRNIYPNFV   KLKAREEYHLT
Sbjct: 398 SSPYLRVYSHCTRNDEGVTVLLINLSNSTTHDVKIVNDRNIYPNFV-GKKLKAREEYHLT 457

Query: 123 PKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYVFVSIKDFKASAC 182
           PKDG+IQSDVMLLNGTPLQLTP L+IP M PKLVDPTS VSVAP SYVFVSIKDFKASAC
Sbjct: 458 PKDGDIQSDVMLLNGTPLQLTPMLEIPKMKPKLVDPTSAVSVAPHSYVFVSIKDFKASAC 517

Query: 183 A 184
           A
Sbjct: 518 A 517

BLAST of Lsi04G003760.1 vs. ExPASy TrEMBL
Match: A0A6J1DCI6 (heparanase-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111019094 PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 6.1e-84
Identity = 153/181 (84.53%), Postives = 168/181 (92.82%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLDQLGMTAT+NH  YCRQALIGGNYALLNTTTFIPNPDYY ALLWHRLMGKKVLS SHD
Sbjct: 339 YLDQLGMTATYNHHAYCRQALIGGNYALLNTTTFIPNPDYYGALLWHRLMGKKVLSVSHD 398

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSKLKAREEYHLT 122
            SPYLRVYSHC+KN  G+T+LLINMSNSTTFDVNIVNDR +YP+  +  +LKAREEYHL+
Sbjct: 399 GSPYLRVYSHCSKNRSGITVLLINMSNSTTFDVNIVNDRKLYPS-CNGGRLKAREEYHLS 458

Query: 123 PKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYVFVSIKDFKASAC 182
           PKDG+IQSDV+LLNGTPL+LTPTLDIPDMSPKLVDPTS VSV+PDSYVFV+IKDF+ASAC
Sbjct: 459 PKDGDIQSDVVLLNGTPLRLTPTLDIPDMSPKLVDPTSDVSVSPDSYVFVTIKDFRASAC 518

Query: 183 A 184
           A
Sbjct: 519 A 518

BLAST of Lsi04G003760.1 vs. NCBI nr
Match: XP_038893314.1 (heparanase-like protein 1 [Benincasa hispida])

HSP 1 Score: 350.5 bits (898), Expect = 8.7e-93
Identity = 171/181 (94.48%), Postives = 177/181 (97.79%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLDQLGMTATFNHQVYCRQALIGGNYALLNTT+FIPNPDYYSALLWHRLMGKKVL ASHD
Sbjct: 341 YLDQLGMTATFNHQVYCRQALIGGNYALLNTTSFIPNPDYYSALLWHRLMGKKVLLASHD 400

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSKLKAREEYHLT 122
           SSPYLRVYSHCTKNNEGVT++LINMSNSTTFDVNIVNDRN+YPNF+  SKLKAREEYHLT
Sbjct: 401 SSPYLRVYSHCTKNNEGVTVVLINMSNSTTFDVNIVNDRNVYPNFI-GSKLKAREEYHLT 460

Query: 123 PKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYVFVSIKDFKASAC 182
           PKDGNIQSDVMLLNG+PLQLTPTLDIPDMSPKLVDPTS VSVAPDSYVFVSIKDFKASAC
Sbjct: 461 PKDGNIQSDVMLLNGSPLQLTPTLDIPDMSPKLVDPTSAVSVAPDSYVFVSIKDFKASAC 520

Query: 183 A 184
           A
Sbjct: 521 A 520

BLAST of Lsi04G003760.1 vs. NCBI nr
Match: TYK09015.1 (heparanase-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 333.6 bits (854), Expect = 1.1e-87
Identity = 165/181 (91.16%), Postives = 172/181 (95.03%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLD LGMTA+F+HQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVL+ASHD
Sbjct: 209 YLDNLGMTASFDHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLAASHD 268

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSKLKAREEYHLT 122
           SSPYLRVYSHCTKN EGVT+LLIN+SNSTT DV IVNDRNIYPNFV  SKLKAREEYHLT
Sbjct: 269 SSPYLRVYSHCTKNEEGVTVLLINLSNSTTHDVKIVNDRNIYPNFV-GSKLKAREEYHLT 328

Query: 123 PKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYVFVSIKDFKASAC 182
           PKDG+IQSDVMLLNGTPLQLTPTLDIP MSPKLVDPTS V+VAP SYVFVSIKDFKASAC
Sbjct: 329 PKDGDIQSDVMLLNGTPLQLTPTLDIPKMSPKLVDPTSVVTVAPSSYVFVSIKDFKASAC 388

Query: 183 A 184
           A
Sbjct: 389 A 388

BLAST of Lsi04G003760.1 vs. NCBI nr
Match: KAA0034459.1 (heparanase-like protein 1 [Cucumis melo var. makuwa] >KAA0034460.1 heparanase-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 333.6 bits (854), Expect = 1.1e-87
Identity = 165/181 (91.16%), Postives = 172/181 (95.03%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLD LGMTA+F+HQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVL+ASHD
Sbjct: 398 YLDNLGMTASFDHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLAASHD 457

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSKLKAREEYHLT 122
           SSPYLRVYSHCTKN EGVT+LLIN+SNSTT DV IVNDRNIYPNFV  SKLKAREEYHLT
Sbjct: 458 SSPYLRVYSHCTKNEEGVTVLLINLSNSTTHDVKIVNDRNIYPNFV-GSKLKAREEYHLT 517

Query: 123 PKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYVFVSIKDFKASAC 182
           PKDG+IQSDVMLLNGTPLQLTPTLDIP MSPKLVDPTS V+VAP SYVFVSIKDFKASAC
Sbjct: 518 PKDGDIQSDVMLLNGTPLQLTPTLDIPKMSPKLVDPTSVVTVAPSSYVFVSIKDFKASAC 577

Query: 183 A 184
           A
Sbjct: 578 A 577

BLAST of Lsi04G003760.1 vs. NCBI nr
Match: XP_008446419.1 (PREDICTED: heparanase-like protein 1 [Cucumis melo])

HSP 1 Score: 333.6 bits (854), Expect = 1.1e-87
Identity = 165/181 (91.16%), Postives = 172/181 (95.03%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLD LGMTA+F+HQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVL+ASHD
Sbjct: 338 YLDNLGMTASFDHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLAASHD 397

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSKLKAREEYHLT 122
           SSPYLRVYSHCTKN EGVT+LLIN+SNSTT DV IVNDRNIYPNFV  SKLKAREEYHLT
Sbjct: 398 SSPYLRVYSHCTKNEEGVTVLLINLSNSTTHDVKIVNDRNIYPNFV-GSKLKAREEYHLT 457

Query: 123 PKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYVFVSIKDFKASAC 182
           PKDG+IQSDVMLLNGTPLQLTPTLDIP MSPKLVDPTS V+VAP SYVFVSIKDFKASAC
Sbjct: 458 PKDGDIQSDVMLLNGTPLQLTPTLDIPKMSPKLVDPTSVVTVAPSSYVFVSIKDFKASAC 517

Query: 183 A 184
           A
Sbjct: 518 A 517

BLAST of Lsi04G003760.1 vs. NCBI nr
Match: XP_011656257.2 (heparanase-like protein 1 [Cucumis sativus])

HSP 1 Score: 327.8 bits (839), Expect = 6.0e-86
Identity = 161/181 (88.95%), Postives = 170/181 (93.92%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLD LGMTA+F+HQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVL+ASHD
Sbjct: 338 YLDNLGMTASFDHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLAASHD 397

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSKLKAREEYHLT 122
           SSPYLRVYSHCT+N+EGVT+LLIN+SNSTT DV IVNDRNIYPNFV   KLKAREEYHLT
Sbjct: 398 SSPYLRVYSHCTRNDEGVTVLLINLSNSTTHDVKIVNDRNIYPNFV-GKKLKAREEYHLT 457

Query: 123 PKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPDSYVFVSIKDFKASAC 182
           PKDG+IQSDVMLLNGTPLQLTP L+IP M PKLVDPTS VSVAP SYVFVSIKDFKASAC
Sbjct: 458 PKDGDIQSDVMLLNGTPLQLTPMLEIPKMKPKLVDPTSAVSVAPHSYVFVSIKDFKASAC 517

Query: 183 A 184
           A
Sbjct: 518 A 517

BLAST of Lsi04G003760.1 vs. TAIR 10
Match: AT5G07830.1 (glucuronidase 2 )

HSP 1 Score: 184.9 bits (468), Expect = 5.9e-47
Identity = 101/202 (50.00%), Postives = 126/202 (62.38%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLDQLGM+A  N +VYCRQ L+GG Y LL   TF+PNPDYYSALLWHRLMGK VL+   D
Sbjct: 342 YLDQLGMSARHNTKVYCRQTLVGGFYGLLEKGTFVPNPDYYSALLWHRLMGKGVLAVQTD 401

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIYPNFVSSSK------LKA- 122
             P LRVY+HC+K   GVTLLLIN+SN + F V++ N  N+  N  S  K      LK  
Sbjct: 402 GPPQLRVYAHCSKGRAGVTLLLINLSNQSDFTVSVSNGINVVLNAESRKKKSLLDTLKRP 461

Query: 123 --------------REEYHLTPKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSG 182
                         REEYHLTP++G ++S  M+LNG  L+ T T DIP + P L    S 
Sbjct: 462 FSWIGSKASDGYLNREEYHLTPENGVLRSKTMVLNGKSLKPTATGDIPSLEPVLRSVNSP 521

Query: 183 VSVAPDSYVFVSIKDFKASACA 184
           ++V P S  F+ + +F ASAC+
Sbjct: 522 LNVLPLSMSFIVLPNFDASACS 543

BLAST of Lsi04G003760.1 vs. TAIR 10
Match: AT5G61250.2 (glucuronidase 1 )

HSP 1 Score: 177.2 bits (448), Expect = 1.2e-44
Identity = 95/202 (47.03%), Postives = 122/202 (60.40%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLDQLG+++  N +VYCRQAL+GG Y LL   TF+PNPDYYSALLWHRLMGK +L     
Sbjct: 338 YLDQLGISSKHNTKVYCRQALVGGFYGLLEKETFVPNPDYYSALLWHRLMGKGILGVQTT 397

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIY--------PNFVSSSKLK 122
           +S YLR Y HC+K   G+T+LLIN+S  TTF V + N   +          +F+ + K K
Sbjct: 398 ASEYLRAYVHCSKRRAGITILLINLSKHTTFTVAVSNGVKVVLQAESMKRKSFLETIKSK 457

Query: 123 A-------------REEYHLTPKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSG 182
                         REEYHL+PKDG+++S +MLLNG PL  T T DIP + P      S 
Sbjct: 458 VSWVGNKASDGYLNREEYHLSPKDGDLRSKIMLLNGKPLVPTATGDIPKLEPVRHGVKSP 517

Query: 183 VSVAPDSYVFVSIKDFKASACA 184
           V + P S  F+ +  F A AC+
Sbjct: 518 VYINPLSISFIVLPTFDAPACS 539

BLAST of Lsi04G003760.1 vs. TAIR 10
Match: AT5G61250.1 (glucuronidase 1 )

HSP 1 Score: 177.2 bits (448), Expect = 1.2e-44
Identity = 95/202 (47.03%), Postives = 122/202 (60.40%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLDQLG+++  N +VYCRQAL+GG Y LL   TF+PNPDYYSALLWHRLMGK +L     
Sbjct: 338 YLDQLGISSKHNTKVYCRQALVGGFYGLLEKETFVPNPDYYSALLWHRLMGKGILGVQTT 397

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTTFDVNIVNDRNIY--------PNFVSSSKLK 122
           +S YLR Y HC+K   G+T+LLIN+S  TTF V + N   +          +F+ + K K
Sbjct: 398 ASEYLRAYVHCSKRRAGITILLINLSKHTTFTVAVSNGVKVVLQAESMKRKSFLETIKSK 457

Query: 123 A-------------REEYHLTPKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSG 182
                         REEYHL+PKDG+++S +MLLNG PL  T T DIP + P      S 
Sbjct: 458 VSWVGNKASDGYLNREEYHLSPKDGDLRSKIMLLNGKPLVPTATGDIPKLEPVRHGVKSP 517

Query: 183 VSVAPDSYVFVSIKDFKASACA 184
           V + P S  F+ +  F A AC+
Sbjct: 518 VYINPLSISFIVLPTFDAPACS 539

BLAST of Lsi04G003760.1 vs. TAIR 10
Match: AT5G34940.1 (glucuronidase 3 )

HSP 1 Score: 169.5 bits (428), Expect = 2.6e-42
Identity = 85/196 (43.37%), Postives = 120/196 (61.22%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLDQLGM + ++ + YCRQ+LIGGNY LLNTT F PNPDYYSAL+W +LMG+K L  +  
Sbjct: 206 YLDQLGMASLYDTKTYCRQSLIGGNYGLLNTTNFTPNPDYYSALIWRQLMGRKALFTTFS 265

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTT----------FDVNIVNDRNIYPN-----F 122
            +  +R Y+HC + ++G+T+LL+N+ N+TT          F +        Y       F
Sbjct: 266 GTKKIRSYTHCARQSKGITVLLMNLDNTTTVVAKVELNNSFSLRHTKHMKSYKRASSQLF 325

Query: 123 VSSSKLKAREEYHLTPKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPD 182
              + +  REEYHLT KDGN+ S  MLLNG  LQ+    D+P + P  ++ T  +++AP 
Sbjct: 326 GGPNGVIQREEYHLTAKDGNLHSQTMLLNGNALQVNSMGDLPPIEPIHINSTEPITIAPY 385

Query: 183 SYVFVSIKDFKASACA 184
           S VFV +++    ACA
Sbjct: 386 SIVFVHMRNVVVPACA 401

BLAST of Lsi04G003760.1 vs. TAIR 10
Match: AT5G34940.2 (glucuronidase 3 )

HSP 1 Score: 169.5 bits (428), Expect = 2.6e-42
Identity = 85/196 (43.37%), Postives = 120/196 (61.22%), Query Frame = 0

Query: 3   YLDQLGMTATFNHQVYCRQALIGGNYALLNTTTFIPNPDYYSALLWHRLMGKKVLSASHD 62
           YLDQLGM + ++ + YCRQ+LIGGNY LLNTT F PNPDYYSAL+W +LMG+K L  +  
Sbjct: 341 YLDQLGMASLYDTKTYCRQSLIGGNYGLLNTTNFTPNPDYYSALIWRQLMGRKALFTTFS 400

Query: 63  SSPYLRVYSHCTKNNEGVTLLLINMSNSTT----------FDVNIVNDRNIYPN-----F 122
            +  +R Y+HC + ++G+T+LL+N+ N+TT          F +        Y       F
Sbjct: 401 GTKKIRSYTHCARQSKGITVLLMNLDNTTTVVAKVELNNSFSLRHTKHMKSYKRASSQLF 460

Query: 123 VSSSKLKAREEYHLTPKDGNIQSDVMLLNGTPLQLTPTLDIPDMSPKLVDPTSGVSVAPD 182
              + +  REEYHLT KDGN+ S  MLLNG  LQ+    D+P + P  ++ T  +++AP 
Sbjct: 461 GGPNGVIQREEYHLTAKDGNLHSQTMLLNGNALQVNSMGDLPPIEPIHINSTEPITIAPY 520

Query: 183 SYVFVSIKDFKASACA 184
           S VFV +++    ACA
Sbjct: 521 SIVFVHMRNVVVPACA 536

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FF108.3e-4650.00Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1[more]
Q8L6081.7e-4347.03Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1[more]
Q9FZP13.6e-4143.37Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2[more]
Q9LRC81.2e-3343.02Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 S... [more]
Q9MYY01.4e-1333.85Heparanase OS=Bos taurus OX=9913 GN=HPSE PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7SZC55.3e-8891.16Heparanase-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A5D3CBY15.3e-8891.16Heparanase-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3BEI85.3e-8891.16heparanase-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103489169 PE=3 SV=1[more]
A0A0A0KQS32.9e-8688.95Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G606560 PE=3 SV=1[more]
A0A6J1DCI66.1e-8484.53heparanase-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111019094 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_038893314.18.7e-9394.48heparanase-like protein 1 [Benincasa hispida][more]
TYK09015.11.1e-8791.16heparanase-like protein 1 [Cucumis melo var. makuwa][more]
KAA0034459.11.1e-8791.16heparanase-like protein 1 [Cucumis melo var. makuwa] >KAA0034460.1 heparanase-li... [more]
XP_008446419.11.1e-8791.16PREDICTED: heparanase-like protein 1 [Cucumis melo][more]
XP_011656257.26.0e-8688.95heparanase-like protein 1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT5G07830.15.9e-4750.00glucuronidase 2 [more]
AT5G61250.21.2e-4447.03glucuronidase 1 [more]
AT5G61250.11.2e-4447.03glucuronidase 1 [more]
AT5G34940.12.6e-4243.37glucuronidase 3 [more]
AT5G34940.22.6e-4243.37glucuronidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 1..50
e-value: 3.3E-7
score: 32.0
NoneNo IPR availablePANTHERPTHR14363:SF13OS07G0598400 PROTEINcoord: 2..183
NoneNo IPR availablePANTHERPTHR14363HEPARANASE-RELATEDcoord: 2..183
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 3..52

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi04G003760Lsi04G003760gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi04G003760.1.exon.1Lsi04G003760.1.exon.1exon
Lsi04G003760.1.exon.2Lsi04G003760.1.exon.2exon
Lsi04G003760.1.exon.3Lsi04G003760.1.exon.3exon
Lsi04G003760.1.exon.4Lsi04G003760.1.exon.4exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi04G003760.1.CDS.1Lsi04G003760.1.CDS.1CDS
Lsi04G003760.1.CDS.2Lsi04G003760.1.CDS.2CDS
Lsi04G003760.1.CDS.3Lsi04G003760.1.CDS.3CDS
Lsi04G003760.1.CDS.4Lsi04G003760.1.CDS.4CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi04G003760.1Lsi04G003760.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0004566 beta-glucuronidase activity