Lsi03G019660.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi03G019660.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr03: 31076183 .. 31079151 (+)
Sequence length1559
RNA-Seq ExpressionLsi03G019660.1
SyntenyLsi03G019660.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATAAATATATCTCAATCTTTTCCTTCCAATTCTCCACTAAACACTCGAATCCATTTTTCAATTTTATTTTTCTTACCTTTAATTTCTCTCTCTCAATTCTAATTCAGGTGGCTGCATGTTCTAATTCCTTTTTTTTTCCTTCTCTATGTTCCATGCACTGTTTGTTTGTTTGTTAATAATGAGTTGTTTTTTCTTTTGTAGTTTTGAAAGATGGCTCCGGGAATGGCAGTTGAGAAAGACAATACCGTTATTGCACCCGAAGCTCAAATGTACCAAAAAGGTGTGAAGCATCTTTGTGACAACGGCTTGAATAAGCTCCCTTCCAAATACGTCCTCCCCAACTCCGATCGCCCCGACGCCTACAAATCCGCCCACCTCGATGTCTTGGGTTCTCTCGAACTTCCCGTCATTGACTTCTCACAACTCTGTGGCCCCAACCGCCGCCATGTCCTTCGTTCCCTCACCAAAGCCTGCGAAGAATATGGCTTCTTTCAGGTTGGTTGATGAATTACACTAAATTTAATCCTTTTTAAGTTCACTCTTATGATGACACAAGAGAAAAAGTTTAAAATTCTTTTAGAAAAAAGAAATGGAAGAGAAGTTTTATGCACAAGTCCAATCCAACTAAATAACATGGAAAATACGACCAGCTAATTGAGTTGAGAAAATTTTCAGTTATTGATCACTGCTAAATTTTTTACACGTGTTTACTGTGATATTTGATTGTGTAGTTAATAAACCATGGAATTTCAAGTGATGTGATATCAAGCATGAGAGAAGTTTGTTCAAAATTCTTCGAGCTTCCATACGAGGAAAGAGCCAAATACATGTCATCCGACATGCATGCGACAGTACGTTATGGGACAAGTTTCAACCAAAACAAAGACAATGTTTTTTGTTGGAGGGACTTCTTGAAGTTGACTTGCAACCCTAATTTGCACCAGCTTCTCTCCGGCTGGCCATCATCTCCGGCAGGATTCAAAGACTCGGCGTCTACGTACGCGGAGGAAACCCGAGGGCTGTTCCTAAGTCTGGTGGAAGCCCTTCTTGAAAGCTTAGGAAGAGATGATCATATGGATGAGAAAGAAGAAAAATTGTTGAAGGAGTTTGAAAAAGGAAGCCAATTAATGGTGATGAATTGTTATCCACAATGTCCTGAGCCAGAGCTAACCCTAGGATTGCCACCTCACTCTGATTATGGCTTCCTCACTCTTCTTCTTCAAGATCAAGTTGAAGGACTTCAAATTCATTACCAACAAAAATGGGTCACTGTTCAACCTTTGCCCAATTCCTTTGTTGTCAACATTGGTGATCATCTAGAGGTGGGTTCTTCTTTTATGTTCCTCCTCCTTCTTTAATTTATTAATTAACAAATATATTAGGCTTTACCTGCTCTTACCGACTTTCTGGGGTTAATTGGATGGGAGGGTTAATTTCTGATATGATTCATTATAGTATGTTTTTAGTGGTGATTTTAATATTATATTTCTGACTTTCTGATACGATTCACTGTAATTTATTTTTTGTGGTGCTTTTTTAATACTATATTATATTAAGAGAAAACACGTTAATTTTTTTTACTAAACCTTTTAGTCTTTTTATCGTAAAAAATTAGGTCCTCAAATTTCTACCAATGTTAATAACCTGGACCCCAAATAATAAAAATTGAACTCTCAAACTTACAAAATTGTTACAATTTCTACAATTATATAAGTTTGAGGGTCTAATTGAAAGTTTGATGGTATAATTATAACTACTACCATACTTTAGGATTGATTTTTGCCATTTTCCCTAAAAAATTTATTGGGTAACATATTTTTTTCTAAAATAGTTGTAAATATGTGTATATTTAAAAAGAAACACACACAATAATCACTAGTTTAGTTAATAATAGTATTCATTTTAACTTCTGTTAACATTTTTATTCCATAATTAGGTAATTAAATTTATATGGTTAATGATTGGTTTTGGCATTATGACTAATTAAAAGAAATTCTTAATTAGTGATTAAATTTGTTAAAGTTTGTATCAAACTTTGATATTGCCTTTTTCAAGTTGTGTCATGTCTTTAATGTACTATCTAACATTTATTAATATGCTATATTTGCAATTTGTTTCACTAAATTGTCCTAAAATAATAATAATAATAATAATAATTTCATCCACCAGTCTATATATATATTCAGTAGATATGCCACTAACACTTAGAAATCCAACAGAACCAGACTGTAAAACAAAAAGTGGACTGTTTGTATTTGTTTAATATATATGCTATAAATGCATACAAATTAGAAGTCAAATTTTAGCTATAGGGATCTCTCTCTATATATATATAATTTGCAATTGGGGGTTTAATAATAATAATAATAATAATTTTGGTAAATAATTTTTTTTAACGACAGATATTCAGCAATGGAAGGTACAAGAGTGTTCTACATAGAGTTGTGGTGAATGCAAAGAAGGCAAGAATGTCAGTGGCAACATTACATAGCCTTCCTTTTGACTCAACAATTAAGCCATGGCCAAAGCTTGTGAATGAAGAGAATCCAAGCCTTTATAAAGACACTGATTTTGGTATCTTCCTTGATTACATTTCCTCTTGTGAGCCCAAGAGCAAGAACTTCCTAGAATCTAGGAAAATTATTTATTGACTAAATATTTTAATTTATTAGTACAATATAATGTCAGAAAAAAAAAATTATAGATGTTGATATAATCAATTTAGTTTATTTTGTGGGTCTCAAGGGTAACGTTGGAAAAAGGTAAAACCCAATGAAACCCTAGTTTTATAGATGATATATATGGCAAAAAATTGTCCCTTTTTGTATAATTGGAATGTGAAAGAGAAGAATTTGAGAAGATATAACCTTTAGTGTATTATTGTTGCTTGATTCATTGCATACCGTGTAAGTGTACAGTGTCAAGTTGTAATATAACGATGGAATAAAAAAGAGTATTGTTTGATAG

mRNA sequence

CTATAAATATATCTCAATCTTTTCCTTCCAATTCTCCACTAAACACTCGAATCCATTTTTCAATTTTATTTTTCTTACCTTTAATTTCTCTCTCTCAATTCTAATTCAGTTTTGAAAGATGGCTCCGGGAATGGCAGTTGAGAAAGACAATACCGTTATTGCACCCGAAGCTCAAATGTACCAAAAAGGTGTGAAGCATCTTTGTGACAACGGCTTGAATAAGCTCCCTTCCAAATACGTCCTCCCCAACTCCGATCGCCCCGACGCCTACAAATCCGCCCACCTCGATGTCTTGGGTTCTCTCGAACTTCCCGTCATTGACTTCTCACAACTCTGTGGCCCCAACCGCCGCCATGTCCTTCGTTCCCTCACCAAAGCCTGCGAAGAATATGGCTTCTTTCAGTTAATAAACCATGGAATTTCAAGTGATGTGATATCAAGCATGAGAGAAGTTTGTTCAAAATTCTTCGAGCTTCCATACGAGGAAAGAGCCAAATACATGTCATCCGACATGCATGCGACAGTACGTTATGGGACAAGTTTCAACCAAAACAAAGACAATGTTTTTTGTTGGAGGGACTTCTTGAAGTTGACTTGCAACCCTAATTTGCACCAGCTTCTCTCCGGCTGGCCATCATCTCCGGCAGGATTCAAAGACTCGGCGTCTACGTACGCGGAGGAAACCCGAGGGCTGTTCCTAAGTCTGGTGGAAGCCCTTCTTGAAAGCTTAGGAAGAGATGATCATATGGATGAGAAAGAAGAAAAATTGTTGAAGGAGTTTGAAAAAGGAAGCCAATTAATGGTGATGAATTGTTATCCACAATGTCCTGAGCCAGAGCTAACCCTAGGATTGCCACCTCACTCTGATTATGGCTTCCTCACTCTTCTTCTTCAAGATCAAGTTGAAGGACTTCAAATTCATTACCAACAAAAATGGGTCACTGTTCAACCTTTGCCCAATTCCTTTGTTGTCAACATTGGTGATCATCTAGAGATATTCAGCAATGGAAGGTACAAGAGTGTTCTACATAGAGTTGTGGTGAATGCAAAGAAGGCAAGAATGTCAGTGGCAACATTACATAGCCTTCCTTTTGACTCAACAATTAAGCCATGGCCAAAGCTTGTGAATGAAGAGAATCCAAGCCTTTATAAAGACACTGATTTTGGTATCTTCCTTGATTACATTTCCTCTTGTGAGCCCAAGAGCAAGAACTTCCTAGAATCTAGGAAAATTATTTATTGACTAAATATTTTAATTTATTAGTACAATATAATGTCAGAAAAAAAAAATTATAGATGTTGATATAATCAATTTAGTTTATTTTGTGGGTCTCAAGGGTAACGTTGGAAAAAGGTAAAACCCAATGAAACCCTAGTTTTATAGATGATATATATGGCAAAAAATTGTCCCTTTTTGTATAATTGGAATGTGAAAGAGAAGAATTTGAGAAGATATAACCTTTAGTGTATTATTGTTGCTTGATTCATTGCATACCGTGTAAGTGTACAGTGTCAAGTTGTAATATAACGATGGAATAAAAAAGAGTATTGTTTGATAG

Coding sequence (CDS)

ATGGCTCCGGGAATGGCAGTTGAGAAAGACAATACCGTTATTGCACCCGAAGCTCAAATGTACCAAAAAGGTGTGAAGCATCTTTGTGACAACGGCTTGAATAAGCTCCCTTCCAAATACGTCCTCCCCAACTCCGATCGCCCCGACGCCTACAAATCCGCCCACCTCGATGTCTTGGGTTCTCTCGAACTTCCCGTCATTGACTTCTCACAACTCTGTGGCCCCAACCGCCGCCATGTCCTTCGTTCCCTCACCAAAGCCTGCGAAGAATATGGCTTCTTTCAGTTAATAAACCATGGAATTTCAAGTGATGTGATATCAAGCATGAGAGAAGTTTGTTCAAAATTCTTCGAGCTTCCATACGAGGAAAGAGCCAAATACATGTCATCCGACATGCATGCGACAGTACGTTATGGGACAAGTTTCAACCAAAACAAAGACAATGTTTTTTGTTGGAGGGACTTCTTGAAGTTGACTTGCAACCCTAATTTGCACCAGCTTCTCTCCGGCTGGCCATCATCTCCGGCAGGATTCAAAGACTCGGCGTCTACGTACGCGGAGGAAACCCGAGGGCTGTTCCTAAGTCTGGTGGAAGCCCTTCTTGAAAGCTTAGGAAGAGATGATCATATGGATGAGAAAGAAGAAAAATTGTTGAAGGAGTTTGAAAAAGGAAGCCAATTAATGGTGATGAATTGTTATCCACAATGTCCTGAGCCAGAGCTAACCCTAGGATTGCCACCTCACTCTGATTATGGCTTCCTCACTCTTCTTCTTCAAGATCAAGTTGAAGGACTTCAAATTCATTACCAACAAAAATGGGTCACTGTTCAACCTTTGCCCAATTCCTTTGTTGTCAACATTGGTGATCATCTAGAGATATTCAGCAATGGAAGGTACAAGAGTGTTCTACATAGAGTTGTGGTGAATGCAAAGAAGGCAAGAATGTCAGTGGCAACATTACATAGCCTTCCTTTTGACTCAACAATTAAGCCATGGCCAAAGCTTGTGAATGAAGAGAATCCAAGCCTTTATAAAGACACTGATTTTGGTATCTTCCTTGATTACATTTCCTCTTGTGAGCCCAAGAGCAAGAACTTCCTAGAATCTAGGAAAATTATTTATTGA

Protein sequence

MAPGMAVEKDNTVIAPEAQMYQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHLDVLGSLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFELPYEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRDFLKLTCNPNLHQLLSGWPSSPAGFKDSASTYAEETRGLFLSLVEALLESLGRDDHMDEKEEKLLKEFEKGSQLMVMNCYPQCPEPELTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISSCEPKSKNFLESRKIIY
Homology
BLAST of Lsi03G019660.1 vs. ExPASy Swiss-Prot
Match: Q6YYX9 (Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC1 PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 6.3e-109
Identity = 183/352 (51.99%), Postives = 253/352 (71.88%), Query Frame = 0

Query: 22  QKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHLDVLGSLELPVIDFSQLCGPNRR-HV 81
           ++GV+HLCD+G+ +LP  YVLP SDRP     A     GS++LPV+D S+L  P+ R  V
Sbjct: 38  RRGVRHLCDSGITRLPGNYVLPASDRPGQAAGAAAAAGGSVKLPVVDLSRLRVPSERGAV 97

Query: 82  LRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFELPYEERAKYMSSDMHATVRYGT 141
           LR+L  AC EYGFFQ++NHG+  +V+  M +V  +FFELP  ER +YMS+D+ A VRYGT
Sbjct: 98  LRTLDAACREYGFFQVVNHGVGGEVVGGMLDVARRFFELPQPERERYMSADVRAPVRYGT 157

Query: 142 SFNQNKDNVFCWRDFLKLTCNPNLHQLLSGWPSSPAGFKDSASTYAEETRGLFLSLVEAL 201
           SFNQ +D V CWRDFLKL C P L  ++  WP+SPA  ++ AS YAE  + +F+ ++EA 
Sbjct: 158 SFNQVRDAVLCWRDFLKLACMP-LAAVVESWPTSPADLREVASRYAEANQRVFMEVMEAA 217

Query: 202 LESLGRDDHMDEKEEKLLKEFEKGSQLMVMNCYPQCPEPELTLGLPPHSDYGFLTLLLQD 261
           LE+LG           ++++   G+Q+M +NCYP+CP+PELTLG+PPHSDYGFLTL+LQD
Sbjct: 218 LEALGVGG------GGVMEDLAAGTQMMTVNCYPECPQPELTLGMPPHSDYGFLTLVLQD 277

Query: 262 QVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNAKKARMSVATL 321
           +V GLQ+ +  +W+TV PLP SFVVN+GDHLEI SNGRY+SVLHRV VN+++ R+SVA+ 
Sbjct: 278 EVAGLQVMHAGEWLTVDPLPGSFVVNVGDHLEILSNGRYRSVLHRVKVNSRRLRVSVASF 337

Query: 322 HSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISSCEPKSKNFLESRKI 373
           HS+  +  + P P+L+++ +P  Y DTD   FL Y++S     K+FL SR++
Sbjct: 338 HSVAPERVVSPAPELIDDRHPRRYMDTDLATFLAYLASAAGNHKSFLHSRRL 382

BLAST of Lsi03G019660.1 vs. ExPASy Swiss-Prot
Match: Q651B6 (Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC2 PE=2 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 7.2e-89
Identity = 172/358 (48.04%), Postives = 231/358 (64.53%), Query Frame = 0

Query: 21  YQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHLDVLGSLELPVIDFSQLCGPNRRHV 80
           ++ GV+ LCD+G+  LP++YVLP +DRP  Y      V     LPV+D + L   +    
Sbjct: 21  WELGVRQLCDSGITTLPARYVLPPADRPARY------VTPPALLPVVDLAALRARDPCQ- 80

Query: 81  LRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFELPYEERAKYMSSDMHATVRYGT 140
           L +L  AC +YGFFQL+NHG+  D +  +      FF+LP   R +YMS+D+ A VRYGT
Sbjct: 81  LAALHAACRDYGFFQLLNHGVPPDAM--LYAARRFFFDLPLPARKRYMSADIRAAVRYGT 140

Query: 141 SFNQNKDNVFCWRDFLKLTCNP--NLHQLLSGWPSSPAGFKDSASTYAEETRGLFLSLVE 200
           SFNQ  D V  WRDFLKL       L  +L  WP +P   + +A+ YA   + LF  L+E
Sbjct: 141 SFNQLNDAVLSWRDFLKLLIRDTRRLADVLPSWPDAPDDLRPAAAAYATACQRLFRELME 200

Query: 201 ALLESLGRDDHMDEKEEKLLKEFEKGSQLMVMNCYPQCPEPELTLGLPPHSDYGFLTLLL 260
           A L++LG    +     +LL+E + GSQ+M++NC+P CPEPELTLG+PPHSDYG LT+LL
Sbjct: 201 AALDALG----IVRCRRQLLEECDAGSQMMMVNCFPACPEPELTLGMPPHSDYGLLTILL 260

Query: 261 QDQVEGLQIHYQQ--KWVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNAKKARMS 320
           QD+V GL++ Y     W  V+PLP + VVN+GDHLEI SNG Y+SVLHRV VN ++AR+S
Sbjct: 261 QDEVRGLEVSYGDGGGWAVVEPLPGAVVVNVGDHLEILSNGLYRSVLHRVRVNGRRARVS 320

Query: 321 VATLHSLPFDSTIKPWPKLVNEE--NPSLYKDTDFGIFLDYISSCEPKSKNFLESRKI 373
           VA+LHSL  +  I P  +LV+E+   P  Y DTD   FL Y++S E   K+FL SR+I
Sbjct: 321 VASLHSLAAERVIGPAAELVDEQRGRPRRYMDTDMAAFLAYLASAEGNHKSFLHSRRI 365

BLAST of Lsi03G019660.1 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.8e-72
Identity = 141/321 (43.93%), Postives = 191/321 (59.50%), Query Frame = 0

Query: 36  LPSKYVLPNSDRPDAYKSAHLDVLGSLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFFQ 95
           +PS YV P SDRP+    + ++  G   +P+ID   L GPNR  +++ L  AC  YGFFQ
Sbjct: 20  IPSNYVRPISDRPNL---SEVESSGD-SIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQ 79

Query: 96  LINHGISSDVISSMREVCSKFFELPYEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRDF 155
           + NHG+    ++ M+ V  +FF  P  ER K+ S+D   T R  TSFN   D V  WRDF
Sbjct: 80  IKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDF 139

Query: 156 LKLTCNPNLHQLLSGWPSSPAGFKDSASTYAEETRGLFLSLVEALLESLG-RDDHMDEKE 215
           L+L C P +   +  WPSSP  F++  + YA   R L L L+EA+ ESLG   DH+    
Sbjct: 140 LRLHCFP-IEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHIS--- 199

Query: 216 EKLLKEFEKGSQLMVMNCYPQCPEPELTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQKWV 275
                   K +Q M  N YP CPEPELT GLP H D   +T+LLQDQV GLQ+    KWV
Sbjct: 200 ----NILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWV 259

Query: 276 TVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPK 335
            V P+PN+F+VNIGD +++ SN +YKSVLHR VVN +  R+S+ T +    D+ I P  +
Sbjct: 260 AVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHE 319

Query: 336 LVNEENP-SLYKDTDFGIFLD 355
           LVNE++  ++Y+   F  + D
Sbjct: 320 LVNEQDSLAIYRTYPFVEYWD 328

BLAST of Lsi03G019660.1 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 4.0e-71
Identity = 131/324 (40.43%), Postives = 194/324 (59.88%), Query Frame = 0

Query: 33  LNKLPSKYVLPNSDRPDAYKSAHLDVLGSLELPVIDFSQLCGPNRRHVLRSLTKACEEYG 92
           ++ +PS YV P SDRP   K + +   G   +P+ID   L GPNR  ++     AC   G
Sbjct: 15  VDHVPSNYVRPVSDRP---KMSEVQTSGD-SIPLIDLHDLHGPNRADIINQFAHACSSCG 74

Query: 93  FFQLINHGISSDVISSMREVCSKFFELPYEERAKYMSSDMHATVRYGTSFNQNKDNVFCW 152
           FFQ+ NHG+  + I  M     +FF     ER K+ S+D   T R  TSFN +K+ V  W
Sbjct: 75  FFQIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNW 134

Query: 153 RDFLKLTCNPNLHQLLSGWPSSPAGFKDSASTYAEETRGLFLSLVEALLESLGRDDHMDE 212
           RDFL+L C P +   ++ WPS+P  F++  + YA   R L L+L+EA+ ESLG       
Sbjct: 135 RDFLRLHCYP-IEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGL------ 194

Query: 213 KEEKLLKEFEKGSQLMVMNCYPQCPEPELTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQK 272
            ++++     K  Q M +N YP+CP+PELT GLP H D   +T+LLQD+V GLQ+    K
Sbjct: 195 AKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGK 254

Query: 273 WVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNAKKARMSVATLHSLPFDSTIKPW 332
           W+ V P+PN+F+VN+GD +++ SN +YKSVLHR VVN+   R+S+ T +    D+ I P 
Sbjct: 255 WIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPA 314

Query: 333 PKLVNEE--NPSLYKDTDFGIFLD 355
            +L+NEE  +P++Y++  +  + +
Sbjct: 315 QELINEEEDSPAIYRNFTYAEYFE 327

BLAST of Lsi03G019660.1 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.7e-67
Identity = 137/313 (43.77%), Postives = 188/313 (60.06%), Query Frame = 0

Query: 36  LPSKYVLPNSDRPDAYKSAHLD-VLGSLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFF 95
           +P KYV P S RP       LD V+    +PV+D   L  P+R  V+ ++  AC  +GFF
Sbjct: 20  MPGKYVRPESQRP------RLDLVVSDARIPVVD---LASPDRAAVVSAVGDACRTHGFF 79

Query: 96  QLINHGISSDVISSMREVCSKFFELPYEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRD 155
           Q++NHGI + +I+S+ EV  +FF LP EE+AK  S D    +R  TSFN  K+ V  WRD
Sbjct: 80  QVVNHGIDAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSFNVRKETVHNWRD 139

Query: 156 FLKLTCNPNLHQLLSGWPSSPAGFKDSASTYAEETRGLFLSLVEALLESLGRDDHMDEKE 215
           +L+L C P LHQ +  WPS+P  FK+   TY  E R L   L EA+ ESLG +       
Sbjct: 140 YLRLHCYP-LHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGY---- 199

Query: 216 EKLLKEFEKGSQLMVMNCYPQCPEPELTLGLPPHSDYGFLT-LLLQDQVEGLQIHYQQKW 275
             + +   +  Q M +N YPQCPEPELT GLP H+D   LT LL+ DQV GLQ+    KW
Sbjct: 200 --MRETLGEQEQHMAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKW 259

Query: 276 VTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNAKKARMSVATLHSLPFDSTIKPWP 335
           + V P P + V+NIGD L+  SNG+Y+SV HR VVN+ + RMSVA+         + P  
Sbjct: 260 IAVNPQPGALVINIGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAK 316

Query: 336 KLVNEENPSLYKD 347
           KL+ +++P++Y++
Sbjct: 320 KLITDDSPAVYRN 316

BLAST of Lsi03G019660.1 vs. ExPASy TrEMBL
Match: A0A5D3DPE4 (Protein DMR6-LIKE OXYGENASE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003160 PE=3 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 5.6e-193
Identity = 326/376 (86.70%), Postives = 351/376 (93.35%), Query Frame = 0

Query: 1   MAPGMAVEKDNTVIAPEAQ-MYQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHLDVL 60
           MAPG+AVE  NTV+A EAQ MYQKGVKHLCDNGLNKLPSKY+LPNSDRPD  +S HL V 
Sbjct: 1   MAPGLAVENGNTVMASEAQIMYQKGVKHLCDNGLNKLPSKYILPNSDRPDTCESPHLHVS 60

Query: 61  GSLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFEL 120
           G L+LPVIDF+QL GPNR HV+ SLTKACEEYGFFQ+INHGIS+D I++MREVCSKFFEL
Sbjct: 61  GPLQLPVIDFAQLLGPNRHHVIHSLTKACEEYGFFQVINHGISNDAITNMREVCSKFFEL 120

Query: 121 PYEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRDFLKLTCNPNLHQLLSGWPSSPAGFK 180
           PYEERAKYMSSDMHATVRYGTSFNQNKDNV+CWRDFLKLTCNPNLHQLLS WPSSP+GFK
Sbjct: 121 PYEERAKYMSSDMHATVRYGTSFNQNKDNVYCWRDFLKLTCNPNLHQLLSSWPSSPSGFK 180

Query: 181 DSASTYAEETRGLFLSLVEALLESLGRDDHMDEKEEK-LLKEFEKGSQLMVMNCYPQCPE 240
           DSASTY EETRGLFLSLVEA++ESLG++  +DE EEK LLKEFEKGSQLMV+NCYP+CPE
Sbjct: 181 DSASTYVEETRGLFLSLVEAIVESLGKE--IDENEEKSLLKEFEKGSQLMVVNCYPKCPE 240

Query: 241 PELTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGR 300
           PELTLG+PPHSDYGFLTLLLQDQVEGLQIHY+QKWVTVQPLPNSFVVNIGDHLEIFSNG+
Sbjct: 241 PELTLGMPPHSDYGFLTLLLQDQVEGLQIHYKQKWVTVQPLPNSFVVNIGDHLEIFSNGK 300

Query: 301 YKSVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISS 360
           YKSVLHRVVVNAKK R+SVATLHSLPFDS IKPWPKLVNEENP LY+DTDFG FLDYISS
Sbjct: 301 YKSVLHRVVVNAKKTRISVATLHSLPFDSIIKPWPKLVNEENPRLYRDTDFGTFLDYISS 360

Query: 361 CEPKSKNFLESRKIIY 375
           CEPK KNFLESRKIIY
Sbjct: 361 CEPKRKNFLESRKIIY 374

BLAST of Lsi03G019660.1 vs. ExPASy TrEMBL
Match: A0A1S3B6H1 (protein DMR6-LIKE OXYGENASE 1 OS=Cucumis melo OX=3656 GN=LOC103486538 PE=3 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 5.6e-193
Identity = 326/376 (86.70%), Postives = 351/376 (93.35%), Query Frame = 0

Query: 1   MAPGMAVEKDNTVIAPEAQ-MYQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHLDVL 60
           MAPG+AVE  NTV+A EAQ MYQKGVKHLCDNGLNKLPSKY+LPNSDRPD  +S HL V 
Sbjct: 1   MAPGLAVENGNTVMASEAQIMYQKGVKHLCDNGLNKLPSKYILPNSDRPDTCESPHLHVS 60

Query: 61  GSLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFEL 120
           G L+LPVIDF+QL GPNR HV+ SLTKACEEYGFFQ+INHGIS+D I++MREVCSKFFEL
Sbjct: 61  GPLQLPVIDFAQLLGPNRHHVIHSLTKACEEYGFFQVINHGISNDAITNMREVCSKFFEL 120

Query: 121 PYEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRDFLKLTCNPNLHQLLSGWPSSPAGFK 180
           PYEERAKYMSSDMHATVRYGTSFNQNKDNV+CWRDFLKLTCNPNLHQLLS WPSSP+GFK
Sbjct: 121 PYEERAKYMSSDMHATVRYGTSFNQNKDNVYCWRDFLKLTCNPNLHQLLSSWPSSPSGFK 180

Query: 181 DSASTYAEETRGLFLSLVEALLESLGRDDHMDEKEEK-LLKEFEKGSQLMVMNCYPQCPE 240
           DSASTY EETRGLFLSLVEA++ESLG++  +DE EEK LLKEFEKGSQLMV+NCYP+CPE
Sbjct: 181 DSASTYVEETRGLFLSLVEAIVESLGKE--IDENEEKSLLKEFEKGSQLMVVNCYPKCPE 240

Query: 241 PELTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGR 300
           PELTLG+PPHSDYGFLTLLLQDQVEGLQIHY+QKWVTVQPLPNSFVVNIGDHLEIFSNG+
Sbjct: 241 PELTLGMPPHSDYGFLTLLLQDQVEGLQIHYKQKWVTVQPLPNSFVVNIGDHLEIFSNGK 300

Query: 301 YKSVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISS 360
           YKSVLHRVVVNAKK R+SVATLHSLPFDS IKPWPKLVNEENP LY+DTDFG FLDYISS
Sbjct: 301 YKSVLHRVVVNAKKTRISVATLHSLPFDSIIKPWPKLVNEENPRLYRDTDFGTFLDYISS 360

Query: 361 CEPKSKNFLESRKIIY 375
           CEPK KNFLESRKIIY
Sbjct: 361 CEPKRKNFLESRKIIY 374

BLAST of Lsi03G019660.1 vs. ExPASy TrEMBL
Match: A0A0A0LGG0 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G747620 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 9.2e-188
Identity = 318/377 (84.35%), Postives = 349/377 (92.57%), Query Frame = 0

Query: 1   MAPGMAVEKDNTVIAPEAQMYQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYK-SAHLDVL 60
           MAPG+AVE  NT++A E QMYQKGVKHLCDNGLNKLPSKY+LPNSDRPD    S HL V 
Sbjct: 1   MAPGLAVENGNTLMASEPQMYQKGVKHLCDNGLNKLPSKYILPNSDRPDTSDYSPHLHVS 60

Query: 61  GSLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFEL 120
           G L+LP+IDF+QL GPNR HV+ SLTKAC+EYGFFQ+INHGIS+DVI++MREVCSKFFEL
Sbjct: 61  GPLQLPLIDFAQLLGPNRHHVIHSLTKACQEYGFFQVINHGISNDVITNMREVCSKFFEL 120

Query: 121 PYEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRDFLKLTCNPN-LHQLLSGWPSSPAGF 180
           PYEERAKYMSSDMHATVRYGTSFNQNKDNV+CWRDFLKLTCNPN LHQLLSGWPSSP+GF
Sbjct: 121 PYEERAKYMSSDMHATVRYGTSFNQNKDNVYCWRDFLKLTCNPNDLHQLLSGWPSSPSGF 180

Query: 181 KDSASTYAEETRGLFLSLVEALLESLGRDDHMDEKEEK-LLKEFEKGSQLMVMNCYPQCP 240
           KDSASTY EETRGLFLSLV+A++ESLG++  +DE EE+ LL EFE GSQLMV+NCYP+CP
Sbjct: 181 KDSASTYVEETRGLFLSLVKAIVESLGKE--IDENEEQLLLNEFENGSQLMVVNCYPKCP 240

Query: 241 EPELTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNG 300
           EPELTLG+PPHSDYGFLTLLLQDQVEGLQIHY+QKWVTVQPLPNSFVVNIGDHLEIFSNG
Sbjct: 241 EPELTLGMPPHSDYGFLTLLLQDQVEGLQIHYKQKWVTVQPLPNSFVVNIGDHLEIFSNG 300

Query: 301 RYKSVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYIS 360
           +YKSVLHRVVVNAKK R+SVATLHSLPFDS IKPWPKLVNEENP LY+DTDFG FL+YIS
Sbjct: 301 KYKSVLHRVVVNAKKTRISVATLHSLPFDSIIKPWPKLVNEENPRLYRDTDFGTFLEYIS 360

Query: 361 SCEPKSKNFLESRKIIY 375
           SCEPK KNFLESRKII+
Sbjct: 361 SCEPKRKNFLESRKIIH 375

BLAST of Lsi03G019660.1 vs. ExPASy TrEMBL
Match: A0A6J1KVV0 (flavanone 3-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111498654 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.3e-178
Identity = 307/374 (82.09%), Postives = 337/374 (90.11%), Query Frame = 0

Query: 1   MAPGMAVEKDNTVIAPEAQMYQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHLDVLG 60
           M+P MA E +NT+++ E Q+YQKGVK+LCD GLNKLP KYVLPNSDRP   KS + +V+G
Sbjct: 1   MSPAMAAEMENTIVS-ETQVYQKGVKNLCDKGLNKLPLKYVLPNSDRPGDCKSDNPNVIG 60

Query: 61  SLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFELP 120
           ++ELPVIDF QLCGPNRRHVLRSL+KA E+YGFFQLINH ISS+VISSMREVCSKFFELP
Sbjct: 61  AVELPVIDFVQLCGPNRRHVLRSLSKAGEDYGFFQLINHEISSEVISSMREVCSKFFELP 120

Query: 121 YEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRDFLKLTCNPNLHQLLSGWPSSPAGFKD 180
           YEERAKYMSSDMHATVRYGTSFNQ KD VF WRDFLKLTCNPNLHQLLS WPSSPA FKD
Sbjct: 121 YEERAKYMSSDMHATVRYGTSFNQTKDTVFYWRDFLKLTCNPNLHQLLSNWPSSPAAFKD 180

Query: 181 SASTYAEETRGLFLSLVEALLESLGRDDHMDEKEEKLLKEFEKGSQLMVMNCYPQCPEPE 240
           SASTYAE+TR LFL LVEA+LESLG    M+++EE+LLKEFE GSQLMV+NCYPQCPEPE
Sbjct: 181 SASTYAEQTRRLFLILVEAILESLGA---MEKEEEELLKEFEDGSQLMVVNCYPQCPEPE 240

Query: 241 LTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGRYK 300
           LTLG+PPHSDYGFLTLLLQDQVEGLQIH+QQKWV V+PLPN FVVNIGDHLEIFSNGRYK
Sbjct: 241 LTLGMPPHSDYGFLTLLLQDQVEGLQIHHQQKWVNVKPLPNCFVVNIGDHLEIFSNGRYK 300

Query: 301 SVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISSCE 360
           SVLHRVVVNA + R+SVA+LHSLPF ST+KPWPKLVNEENP  YKDTDFGIFL YI+S E
Sbjct: 301 SVLHRVVVNANEPRISVASLHSLPFQSTMKPWPKLVNEENPRRYKDTDFGIFLQYITSRE 360

Query: 361 PKSKNFLESRKIIY 375
           PK KNFLESRKIIY
Sbjct: 361 PKRKNFLESRKIIY 370

BLAST of Lsi03G019660.1 vs. ExPASy TrEMBL
Match: A0A6J1FA28 (protein DMR6-LIKE OXYGENASE 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442206 PE=3 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.5e-177
Identity = 306/357 (85.71%), Postives = 331/357 (92.72%), Query Frame = 0

Query: 20  MYQKGVKHLCDNGLNKLPSKYVLPNSDRPDA-YKSAHLDVLGSLELPVIDFSQLCGPNRR 79
           MYQKGVKHLCD GLNKLP KYVLP+S+RP A  +SA+LDVLG++ELPVIDF++LCGPNRR
Sbjct: 1   MYQKGVKHLCDKGLNKLPLKYVLPDSERPAAPCQSANLDVLGTIELPVIDFAELCGPNRR 60

Query: 80  HVLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFELPYEERAKYMSSDMHATVRY 139
           HVL SLTKACE+YGFFQL NHGISS+VISSMR+V S+FFELPYEERAKYMSSDMHATVRY
Sbjct: 61  HVLLSLTKACEQYGFFQLTNHGISSEVISSMRDVSSQFFELPYEERAKYMSSDMHATVRY 120

Query: 140 GTSFNQNKDNVFCWRDFLKLTCNPN-LHQLLSGWPSSPAGFKDSASTYAEETRGLFLSLV 199
           GTSFNQNKDNVFCWRDFLKLTCNPN LHQLLS WPSSPAGFKDSASTY EETR LFL LV
Sbjct: 121 GTSFNQNKDNVFCWRDFLKLTCNPNDLHQLLSRWPSSPAGFKDSASTYVEETRALFLGLV 180

Query: 200 EALLESLGRDDHMDEKEEKLLKEFEKGSQLMVMNCYPQCPEPELTLGLPPHSDYGFLTLL 259
           EA++ESLG  +  +E EE LLKEFE GSQLMV+NCYPQCPEPELTLG+PPHSDYGFLTLL
Sbjct: 181 EAIVESLGGMEKEEEMEE-LLKEFEDGSQLMVVNCYPQCPEPELTLGMPPHSDYGFLTLL 240

Query: 260 LQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNAKKARMSV 319
           LQDQVEGLQIH+QQ+WVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRV+VNA K R+SV
Sbjct: 241 LQDQVEGLQIHHQQQWVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVLVNANKPRISV 300

Query: 320 ATLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISSCEPKSKNFLESRKIIY 375
           ATLHSLPFDSTIKPWPKLVNEENP  YKDTDFGIFL+YI+S EPK KNFLESR+IIY
Sbjct: 301 ATLHSLPFDSTIKPWPKLVNEENPPRYKDTDFGIFLEYITSREPKRKNFLESRQIIY 356

BLAST of Lsi03G019660.1 vs. NCBI nr
Match: XP_038905698.1 (protein DMR6-LIKE OXYGENASE 1 [Benincasa hispida] >XP_038905700.1 protein DMR6-LIKE OXYGENASE 1 [Benincasa hispida])

HSP 1 Score: 711.1 bits (1834), Expect = 5.2e-201
Identity = 340/374 (90.91%), Postives = 358/374 (95.72%), Query Frame = 0

Query: 1   MAPGMAVEKDNTVIAPEAQMYQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHLDVLG 60
           M PGMAVE DNTV+A EAQMYQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHLDV  
Sbjct: 1   MTPGMAVENDNTVMALEAQMYQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHLDVPV 60

Query: 61  SLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFELP 120
           +LELPVIDFSQL GPNRR+VLRSLTKACEEYGFFQ+INHGIS+DVIS+MREVCSKFFELP
Sbjct: 61  ALELPVIDFSQLYGPNRRYVLRSLTKACEEYGFFQIINHGISNDVISNMREVCSKFFELP 120

Query: 121 YEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRDFLKLTCNPNLHQLLSGWPSSPAGFKD 180
           YEERAKYMSSDMHATVRYGTSFNQNKDNV+CWRDFLKLTCNPNLH+LLSGWPSSP+GFKD
Sbjct: 121 YEERAKYMSSDMHATVRYGTSFNQNKDNVYCWRDFLKLTCNPNLHELLSGWPSSPSGFKD 180

Query: 181 SASTYAEETRGLFLSLVEALLESLGRDDHMDEKEEKLLKEFEKGSQLMVMNCYPQCPEPE 240
           SASTYAEET+GLFLSLVEA+LESL  D    EK+E LL EFE+GSQLMV+NCYPQCPEPE
Sbjct: 181 SASTYAEETQGLFLSLVEAILESLAGD---MEKDENLLNEFERGSQLMVVNCYPQCPEPE 240

Query: 241 LTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGRYK 300
           LTLG+PPHSDYGFLTLLLQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGRYK
Sbjct: 241 LTLGMPPHSDYGFLTLLLQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGRYK 300

Query: 301 SVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISSCE 360
           SVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPKLVNE+NP LYKDTDFGIFLDYI+SCE
Sbjct: 301 SVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPKLVNEQNPRLYKDTDFGIFLDYITSCE 360

Query: 361 PKSKNFLESRKIIY 375
           PK KNFL+SRKIIY
Sbjct: 361 PKRKNFLDSRKIIY 371

BLAST of Lsi03G019660.1 vs. NCBI nr
Match: XP_008442759.1 (PREDICTED: protein DMR6-LIKE OXYGENASE 1 [Cucumis melo] >TYK25189.1 protein DMR6-LIKE OXYGENASE 1 [Cucumis melo var. makuwa])

HSP 1 Score: 683.3 bits (1762), Expect = 1.2e-192
Identity = 326/376 (86.70%), Postives = 351/376 (93.35%), Query Frame = 0

Query: 1   MAPGMAVEKDNTVIAPEAQ-MYQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHLDVL 60
           MAPG+AVE  NTV+A EAQ MYQKGVKHLCDNGLNKLPSKY+LPNSDRPD  +S HL V 
Sbjct: 1   MAPGLAVENGNTVMASEAQIMYQKGVKHLCDNGLNKLPSKYILPNSDRPDTCESPHLHVS 60

Query: 61  GSLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFEL 120
           G L+LPVIDF+QL GPNR HV+ SLTKACEEYGFFQ+INHGIS+D I++MREVCSKFFEL
Sbjct: 61  GPLQLPVIDFAQLLGPNRHHVIHSLTKACEEYGFFQVINHGISNDAITNMREVCSKFFEL 120

Query: 121 PYEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRDFLKLTCNPNLHQLLSGWPSSPAGFK 180
           PYEERAKYMSSDMHATVRYGTSFNQNKDNV+CWRDFLKLTCNPNLHQLLS WPSSP+GFK
Sbjct: 121 PYEERAKYMSSDMHATVRYGTSFNQNKDNVYCWRDFLKLTCNPNLHQLLSSWPSSPSGFK 180

Query: 181 DSASTYAEETRGLFLSLVEALLESLGRDDHMDEKEEK-LLKEFEKGSQLMVMNCYPQCPE 240
           DSASTY EETRGLFLSLVEA++ESLG++  +DE EEK LLKEFEKGSQLMV+NCYP+CPE
Sbjct: 181 DSASTYVEETRGLFLSLVEAIVESLGKE--IDENEEKSLLKEFEKGSQLMVVNCYPKCPE 240

Query: 241 PELTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGR 300
           PELTLG+PPHSDYGFLTLLLQDQVEGLQIHY+QKWVTVQPLPNSFVVNIGDHLEIFSNG+
Sbjct: 241 PELTLGMPPHSDYGFLTLLLQDQVEGLQIHYKQKWVTVQPLPNSFVVNIGDHLEIFSNGK 300

Query: 301 YKSVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISS 360
           YKSVLHRVVVNAKK R+SVATLHSLPFDS IKPWPKLVNEENP LY+DTDFG FLDYISS
Sbjct: 301 YKSVLHRVVVNAKKTRISVATLHSLPFDSIIKPWPKLVNEENPRLYRDTDFGTFLDYISS 360

Query: 361 CEPKSKNFLESRKIIY 375
           CEPK KNFLESRKIIY
Sbjct: 361 CEPKRKNFLESRKIIY 374

BLAST of Lsi03G019660.1 vs. NCBI nr
Match: XP_004137996.1 (protein DMR6-LIKE OXYGENASE 1 [Cucumis sativus] >KGN59031.1 hypothetical protein Csa_000965 [Cucumis sativus])

HSP 1 Score: 666.0 bits (1717), Expect = 1.9e-187
Identity = 318/377 (84.35%), Postives = 349/377 (92.57%), Query Frame = 0

Query: 1   MAPGMAVEKDNTVIAPEAQMYQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYK-SAHLDVL 60
           MAPG+AVE  NT++A E QMYQKGVKHLCDNGLNKLPSKY+LPNSDRPD    S HL V 
Sbjct: 1   MAPGLAVENGNTLMASEPQMYQKGVKHLCDNGLNKLPSKYILPNSDRPDTSDYSPHLHVS 60

Query: 61  GSLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFEL 120
           G L+LP+IDF+QL GPNR HV+ SLTKAC+EYGFFQ+INHGIS+DVI++MREVCSKFFEL
Sbjct: 61  GPLQLPLIDFAQLLGPNRHHVIHSLTKACQEYGFFQVINHGISNDVITNMREVCSKFFEL 120

Query: 121 PYEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRDFLKLTCNPN-LHQLLSGWPSSPAGF 180
           PYEERAKYMSSDMHATVRYGTSFNQNKDNV+CWRDFLKLTCNPN LHQLLSGWPSSP+GF
Sbjct: 121 PYEERAKYMSSDMHATVRYGTSFNQNKDNVYCWRDFLKLTCNPNDLHQLLSGWPSSPSGF 180

Query: 181 KDSASTYAEETRGLFLSLVEALLESLGRDDHMDEKEEK-LLKEFEKGSQLMVMNCYPQCP 240
           KDSASTY EETRGLFLSLV+A++ESLG++  +DE EE+ LL EFE GSQLMV+NCYP+CP
Sbjct: 181 KDSASTYVEETRGLFLSLVKAIVESLGKE--IDENEEQLLLNEFENGSQLMVVNCYPKCP 240

Query: 241 EPELTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNG 300
           EPELTLG+PPHSDYGFLTLLLQDQVEGLQIHY+QKWVTVQPLPNSFVVNIGDHLEIFSNG
Sbjct: 241 EPELTLGMPPHSDYGFLTLLLQDQVEGLQIHYKQKWVTVQPLPNSFVVNIGDHLEIFSNG 300

Query: 301 RYKSVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYIS 360
           +YKSVLHRVVVNAKK R+SVATLHSLPFDS IKPWPKLVNEENP LY+DTDFG FL+YIS
Sbjct: 301 KYKSVLHRVVVNAKKTRISVATLHSLPFDSIIKPWPKLVNEENPRLYRDTDFGTFLEYIS 360

Query: 361 SCEPKSKNFLESRKIIY 375
           SCEPK KNFLESRKII+
Sbjct: 361 SCEPKRKNFLESRKIIH 375

BLAST of Lsi03G019660.1 vs. NCBI nr
Match: XP_023005746.1 (flavanone 3-dioxygenase 2-like [Cucurbita maxima])

HSP 1 Score: 634.8 bits (1636), Expect = 4.7e-178
Identity = 307/374 (82.09%), Postives = 337/374 (90.11%), Query Frame = 0

Query: 1   MAPGMAVEKDNTVIAPEAQMYQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHLDVLG 60
           M+P MA E +NT+++ E Q+YQKGVK+LCD GLNKLP KYVLPNSDRP   KS + +V+G
Sbjct: 1   MSPAMAAEMENTIVS-ETQVYQKGVKNLCDKGLNKLPLKYVLPNSDRPGDCKSDNPNVIG 60

Query: 61  SLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFELP 120
           ++ELPVIDF QLCGPNRRHVLRSL+KA E+YGFFQLINH ISS+VISSMREVCSKFFELP
Sbjct: 61  AVELPVIDFVQLCGPNRRHVLRSLSKAGEDYGFFQLINHEISSEVISSMREVCSKFFELP 120

Query: 121 YEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRDFLKLTCNPNLHQLLSGWPSSPAGFKD 180
           YEERAKYMSSDMHATVRYGTSFNQ KD VF WRDFLKLTCNPNLHQLLS WPSSPA FKD
Sbjct: 121 YEERAKYMSSDMHATVRYGTSFNQTKDTVFYWRDFLKLTCNPNLHQLLSNWPSSPAAFKD 180

Query: 181 SASTYAEETRGLFLSLVEALLESLGRDDHMDEKEEKLLKEFEKGSQLMVMNCYPQCPEPE 240
           SASTYAE+TR LFL LVEA+LESLG    M+++EE+LLKEFE GSQLMV+NCYPQCPEPE
Sbjct: 181 SASTYAEQTRRLFLILVEAILESLGA---MEKEEEELLKEFEDGSQLMVVNCYPQCPEPE 240

Query: 241 LTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGRYK 300
           LTLG+PPHSDYGFLTLLLQDQVEGLQIH+QQKWV V+PLPN FVVNIGDHLEIFSNGRYK
Sbjct: 241 LTLGMPPHSDYGFLTLLLQDQVEGLQIHHQQKWVNVKPLPNCFVVNIGDHLEIFSNGRYK 300

Query: 301 SVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISSCE 360
           SVLHRVVVNA + R+SVA+LHSLPF ST+KPWPKLVNEENP  YKDTDFGIFL YI+S E
Sbjct: 301 SVLHRVVVNANEPRISVASLHSLPFQSTMKPWPKLVNEENPRRYKDTDFGIFLQYITSRE 360

Query: 361 PKSKNFLESRKIIY 375
           PK KNFLESRKIIY
Sbjct: 361 PKRKNFLESRKIIY 370

BLAST of Lsi03G019660.1 vs. NCBI nr
Match: KAG7017399.1 (Protein DMR6-LIKE OXYGENASE 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 634.4 bits (1635), Expect = 6.1e-178
Identity = 307/357 (85.99%), Postives = 331/357 (92.72%), Query Frame = 0

Query: 20  MYQKGVKHLCDNGLNKLPSKYVLPNSDRPDA-YKSAHLDVLGSLELPVIDFSQLCGPNRR 79
           MYQKGVKHLCD GLNKLP KYVLP+S+RP A  +SA+LDVLG++ELPVIDF++LCGPNR 
Sbjct: 1   MYQKGVKHLCDKGLNKLPLKYVLPDSERPAAPCQSANLDVLGTIELPVIDFAELCGPNRH 60

Query: 80  HVLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFELPYEERAKYMSSDMHATVRY 139
           HVLRSLTKACE++GFFQL NHGIS+DVISSMR+V S+FFELPYEERAKYMSSDMHATVRY
Sbjct: 61  HVLRSLTKACEQFGFFQLTNHGISTDVISSMRDVSSQFFELPYEERAKYMSSDMHATVRY 120

Query: 140 GTSFNQNKDNVFCWRDFLKLTCNPN-LHQLLSGWPSSPAGFKDSASTYAEETRGLFLSLV 199
           GTSFNQNKDNVFCWRDFLKLTCNPN LHQLLS WPSSPAGFKDSASTY EETR LFL LV
Sbjct: 121 GTSFNQNKDNVFCWRDFLKLTCNPNDLHQLLSRWPSSPAGFKDSASTYVEETRALFLGLV 180

Query: 200 EALLESLGRDDHMDEKEEKLLKEFEKGSQLMVMNCYPQCPEPELTLGLPPHSDYGFLTLL 259
           EA++ESLG  +  +E EE LLKEFE GSQLMV+NCYPQCPEPELTLG+PPHSDYGFLTLL
Sbjct: 181 EAIVESLGGMEKEEEMEE-LLKEFEDGSQLMVVNCYPQCPEPELTLGMPPHSDYGFLTLL 240

Query: 260 LQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNAKKARMSV 319
           LQDQVEGLQIH+QQ+WVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNA K R+SV
Sbjct: 241 LQDQVEGLQIHHQQQWVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNANKPRISV 300

Query: 320 ATLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISSCEPKSKNFLESRKIIY 375
           ATLHSLPFDSTIKPWPKLVNEENP  YKDTDFGIFL+YI+S EPK KNFLESRKIIY
Sbjct: 301 ATLHSLPFDSTIKPWPKLVNEENPPRYKDTDFGIFLEYITSREPKRKNFLESRKIIY 356

BLAST of Lsi03G019660.1 vs. TAIR 10
Match: AT2G36690.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 484.2 bits (1245), Expect = 9.6e-137
Identity = 236/373 (63.27%), Postives = 289/373 (77.48%), Query Frame = 0

Query: 1   MAPGMAVEKDNTVIAPEAQMYQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHL-DVL 60
           M+P M       V+  E + YQKGVKHLC+NGL K+P+KY+ P  DRP   KS  L    
Sbjct: 1   MSPSMIA----PVMEEENKKYQKGVKHLCENGLTKVPTKYIWPEPDRPILTKSDKLIKPN 60

Query: 61  GSLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFEL 120
            +L+LP+IDF++L GPNR HVLR++ +AC+ YGFFQ++NHG+  DV  +M +VC +FFEL
Sbjct: 61  KNLKLPLIDFAELLGPNRPHVLRTIAEACKTYGFFQVVNHGMEGDVSKNMIDVCKRFFEL 120

Query: 121 PYEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRDFLKLTCNPNLHQLLSGWPSSPAGFK 180
           PYEER+KYMSSDM A VRYGTSFNQ KDNVFCWRDFLKL  +P L   L  WPSSP+ F+
Sbjct: 121 PYEERSKYMSSDMSAPVRYGTSFNQIKDNVFCWRDFLKLYAHP-LPDYLPHWPSSPSDFR 180

Query: 181 DSASTYAEETRGLFLSLVEALLESLGRDDHMDEKEEKLLKEFEKGSQLMVMNCYPQCPEP 240
            SA+TYA+ET+ +F  +V+A+LESL  D       ++  KE E+GSQ++V+NCYP CPEP
Sbjct: 181 SSAATYAKETKEMFEMMVKAILESLEIDG-----SDEAAKELEEGSQVVVVNCYPPCPEP 240

Query: 241 ELTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQKWVTVQPLPNSFVVNIGDHLEIFSNGRY 300
           ELTLG+PPHSDYGFLTLLLQD+VEGLQI Y+ +WVTV P+P SFVVN+GDHLEIFSNGRY
Sbjct: 241 ELTLGMPPHSDYGFLTLLLQDEVEGLQILYRDEWVTVDPIPGSFVVNVGDHLEIFSNGRY 300

Query: 301 KSVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISSC 360
           KSVLHRV+VN+ K R+SVA+LHS P  S +KP PKLV++ NPS Y DTDF  FL YI+S 
Sbjct: 301 KSVLHRVLVNSTKPRISVASLHSFPLTSVVKPSPKLVDKHNPSQYMDTDFTTFLQYITSR 360

Query: 361 EPKSKNFLESRKI 373
           EPK KNFLESRKI
Sbjct: 361 EPKWKNFLESRKI 363

BLAST of Lsi03G019660.1 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 293.5 bits (750), Expect = 2.4e-79
Identity = 156/348 (44.83%), Postives = 219/348 (62.93%), Query Frame = 0

Query: 28  LCDNGLNKLPSKYVLPNSDRPDAYKSAHLDVLGSLE--LPVIDFSQLCGPNRRHV-LRSL 87
           L ++G+ ++P +YVLP S RP    S     LG+ E  LPVID S L  P  R + +  +
Sbjct: 19  LTNSGVPQVPDRYVLPPSQRPALGSS-----LGTSETTLPVIDLSLLHQPFLRSLAIHEI 78

Query: 88  TKACEEYGFFQLINHGISSDVISSMREVCSKFFELPYEERAKYMSSDMHATVRYGTSFNQ 147
           + AC+E+GFFQ+INHGI S V++   +  ++FF+LP EE+   +S+++H  VRYGTS N 
Sbjct: 79  SMACKEFGFFQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVRYGTSLNH 138

Query: 148 NKDNVFCWRDFLKLTCNPNLHQLLSGWPSSPAGFKDSASTYAEETRGLFLSLVEALLESL 207
           + D V  WRDF+K   +P L + +  WPS+P  +KD    YAE T  L   L+EA+ ESL
Sbjct: 139 STDRVHYWRDFIKHYSHP-LSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESL 198

Query: 208 GRDDHMDEKEEKLLKEFEKGSQLMVMNCYPQCPEPELTLGLPPHSDYGFLTLLLQDQVEG 267
           G +      +  L +E E+GSQ+M +NCYP CPEPE+ LG+PPHSD+  LT+LLQ   +G
Sbjct: 199 GLE------KNYLQEEIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSS-KG 258

Query: 268 LQI-HYQQKWVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNAKKARMSVATLHSL 327
           LQI    + WV V  +  + +V +GD +E+ SNG YKSV+HRV VN +  R+S A+LHSL
Sbjct: 259 LQIMDCNKNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSL 318

Query: 328 PFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISSCEPKSKNFLESRK 372
           P    I P PKLVN  N   Y +  F  FL+YISS +   + F+++ K
Sbjct: 319 PLHKKISPAPKLVNPNNAPAYGEFSFNDFLNYISSNDFIQERFIDTIK 353

BLAST of Lsi03G019660.1 vs. TAIR 10
Match: AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.3e-77
Identity = 153/351 (43.59%), Postives = 211/351 (60.11%), Query Frame = 0

Query: 21  YQKGVKHLCDNGLNKLPSKYVLPNSDRPDAYKSAHLDVLGSLELPVIDFSQLCGP-NRRH 80
           Y      L ++G   +P++Y+LP S RP    S       ++ LPVID S L  P  R  
Sbjct: 12  YFTSAMELTESGDPHVPTRYILPPSQRPMLGPSIG---TSTINLPVIDLSFLHDPLLRLC 71

Query: 81  VLRSLTKACEEYGFFQLINHGISSDVISSMREVCSKFFELPYEERAKYMSSDMHATVRYG 140
           V+  +  AC+ +GFFQ+INHGISS V+   ++  ++FF+LP +E+   +S +    VRYG
Sbjct: 72  VIHEIELACKGFGFFQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVRYG 131

Query: 141 TSFNQNKDNVFCWRDFLKLTCNPNLHQLLSGWPSSPAGFKDSASTYAEETRGLFLSLVEA 200
           TS N + D V  WRDF+K   +P L   ++ WPS+P  +K+    YAE T  L   L+EA
Sbjct: 132 TSINHSTDRVHYWRDFIKHYSHP-LSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEA 191

Query: 201 LLESLGRDDHMDEKEEKLLKEFEKGSQLMVMNCYPQCPEPELTLGLPPHSDYGFLTLLLQ 260
           + ESLG +      +  L +E E+GSQ+M +NCYP CPEPE+ LG+PPHSDYG LT+LLQ
Sbjct: 192 ISESLGLE------KNYLQEEIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQ 251

Query: 261 DQVEGLQI-HYQQKWVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNAKKARMSVA 320
              EGLQI      WV V  +  + +V +GD +E+ SNG YKSV+HRV VN    R+S A
Sbjct: 252 SS-EGLQIKDCNNNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFA 311

Query: 321 TLHSLPFDSTIKPWPKLVNEENPSLYKDTDFGIFLDYISSCEPKSKNFLES 370
           +LHSLP    I P  +LVNE  P+ Y +  F  FLDYIS  +   K F+++
Sbjct: 312 SLHSLPMHKKISPATQLVNENKPAAYGEFSFNDFLDYISRNDITQKRFIDT 351

BLAST of Lsi03G019660.1 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 273.9 bits (699), Expect = 2.0e-73
Identity = 141/321 (43.93%), Postives = 191/321 (59.50%), Query Frame = 0

Query: 36  LPSKYVLPNSDRPDAYKSAHLDVLGSLELPVIDFSQLCGPNRRHVLRSLTKACEEYGFFQ 95
           +PS YV P SDRP+    + ++  G   +P+ID   L GPNR  +++ L  AC  YGFFQ
Sbjct: 20  IPSNYVRPISDRPNL---SEVESSGD-SIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQ 79

Query: 96  LINHGISSDVISSMREVCSKFFELPYEERAKYMSSDMHATVRYGTSFNQNKDNVFCWRDF 155
           + NHG+    ++ M+ V  +FF  P  ER K+ S+D   T R  TSFN   D V  WRDF
Sbjct: 80  IKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDF 139

Query: 156 LKLTCNPNLHQLLSGWPSSPAGFKDSASTYAEETRGLFLSLVEALLESLG-RDDHMDEKE 215
           L+L C P +   +  WPSSP  F++  + YA   R L L L+EA+ ESLG   DH+    
Sbjct: 140 LRLHCFP-IEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHIS--- 199

Query: 216 EKLLKEFEKGSQLMVMNCYPQCPEPELTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQKWV 275
                   K +Q M  N YP CPEPELT GLP H D   +T+LLQDQV GLQ+    KWV
Sbjct: 200 ----NILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWV 259

Query: 276 TVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNAKKARMSVATLHSLPFDSTIKPWPK 335
            V P+PN+F+VNIGD +++ SN +YKSVLHR VVN +  R+S+ T +    D+ I P  +
Sbjct: 260 AVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHE 319

Query: 336 LVNEENP-SLYKDTDFGIFLD 355
           LVNE++  ++Y+   F  + D
Sbjct: 320 LVNEQDSLAIYRTYPFVEYWD 328

BLAST of Lsi03G019660.1 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 270.0 bits (689), Expect = 2.8e-72
Identity = 131/324 (40.43%), Postives = 194/324 (59.88%), Query Frame = 0

Query: 33  LNKLPSKYVLPNSDRPDAYKSAHLDVLGSLELPVIDFSQLCGPNRRHVLRSLTKACEEYG 92
           ++ +PS YV P SDRP   K + +   G   +P+ID   L GPNR  ++     AC   G
Sbjct: 15  VDHVPSNYVRPVSDRP---KMSEVQTSGD-SIPLIDLHDLHGPNRADIINQFAHACSSCG 74

Query: 93  FFQLINHGISSDVISSMREVCSKFFELPYEERAKYMSSDMHATVRYGTSFNQNKDNVFCW 152
           FFQ+ NHG+  + I  M     +FF     ER K+ S+D   T R  TSFN +K+ V  W
Sbjct: 75  FFQIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNW 134

Query: 153 RDFLKLTCNPNLHQLLSGWPSSPAGFKDSASTYAEETRGLFLSLVEALLESLGRDDHMDE 212
           RDFL+L C P +   ++ WPS+P  F++  + YA   R L L+L+EA+ ESLG       
Sbjct: 135 RDFLRLHCYP-IEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGL------ 194

Query: 213 KEEKLLKEFEKGSQLMVMNCYPQCPEPELTLGLPPHSDYGFLTLLLQDQVEGLQIHYQQK 272
            ++++     K  Q M +N YP+CP+PELT GLP H D   +T+LLQD+V GLQ+    K
Sbjct: 195 AKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGK 254

Query: 273 WVTVQPLPNSFVVNIGDHLEIFSNGRYKSVLHRVVVNAKKARMSVATLHSLPFDSTIKPW 332
           W+ V P+PN+F+VN+GD +++ SN +YKSVLHR VVN+   R+S+ T +    D+ I P 
Sbjct: 255 WIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPA 314

Query: 333 PKLVNEE--NPSLYKDTDFGIFLD 355
            +L+NEE  +P++Y++  +  + +
Sbjct: 315 QELINEEEDSPAIYRNFTYAEYFE 327

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6YYX96.3e-10951.99Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japoni... [more]
Q651B67.2e-8948.04Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japoni... [more]
Q9ZSA82.8e-7243.93Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1[more]
Q9ZSA74.0e-7140.43Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1[more]
Q8W2X52.7e-6743.77Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1... [more]
Match NameE-valueIdentityDescription
A0A5D3DPE45.6e-19386.70Protein DMR6-LIKE OXYGENASE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3B6H15.6e-19386.70protein DMR6-LIKE OXYGENASE 1 OS=Cucumis melo OX=3656 GN=LOC103486538 PE=3 SV=1[more]
A0A0A0LGG09.2e-18884.35Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
A0A6J1KVV02.3e-17882.09flavanone 3-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111498654 PE=3 ... [more]
A0A6J1FA281.5e-17785.71protein DMR6-LIKE OXYGENASE 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442206... [more]
Match NameE-valueIdentityDescription
XP_038905698.15.2e-20190.91protein DMR6-LIKE OXYGENASE 1 [Benincasa hispida] >XP_038905700.1 protein DMR6-L... [more]
XP_008442759.11.2e-19286.70PREDICTED: protein DMR6-LIKE OXYGENASE 1 [Cucumis melo] >TYK25189.1 protein DMR6... [more]
XP_004137996.11.9e-18784.35protein DMR6-LIKE OXYGENASE 1 [Cucumis sativus] >KGN59031.1 hypothetical protein... [more]
XP_023005746.14.7e-17882.09flavanone 3-dioxygenase 2-like [Cucurbita maxima][more]
KAG7017399.16.1e-17885.99Protein DMR6-LIKE OXYGENASE 1, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
Match NameE-valueIdentityDescription
AT2G36690.19.6e-13763.272-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G44800.12.4e-7944.832-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G60290.11.3e-7743.592-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G10500.12.0e-7343.932-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G10490.12.8e-7240.432-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 84..101
score: 44.44
coord: 248..269
score: 36.36
coord: 283..309
score: 30.98
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 18..372
NoneNo IPR availablePANTHERPTHR47990:SF1721-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE-RELATEDcoord: 18..372
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 22..355
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 64..163
e-value: 4.9E-27
score: 95.0
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 17..355
e-value: 2.2E-100
score: 338.4
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 227..321
e-value: 8.3E-29
score: 100.1
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 223..323
score: 13.201634

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi03G019660Lsi03G019660gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi03G019660.1.exon.4Lsi03G019660.1.exon.4exon
Lsi03G019660.1.exon.3Lsi03G019660.1.exon.3exon
Lsi03G019660.1.exon.2Lsi03G019660.1.exon.2exon
Lsi03G019660.1.exon.1Lsi03G019660.1.exon.1exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi03G019660.1.five_prime_UTR.1Lsi03G019660.1.five_prime_UTR.1five_prime_UTR
Lsi03G019660.1.five_prime_UTR.2Lsi03G019660.1.five_prime_UTR.2five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi03G019660.1.CDS.1Lsi03G019660.1.CDS.1CDS
Lsi03G019660.1.CDS.2Lsi03G019660.1.CDS.2CDS
Lsi03G019660.1.CDS.3Lsi03G019660.1.CDS.3CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi03G019660.1.three_prime_UTR.1Lsi03G019660.1.three_prime_UTR.1three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi03G019660.1Lsi03G019660.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004497 monooxygenase activity