Lsi03G002120.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G002120.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Proline dehydrogenase
Location	chr03: 2724191 .. 2728268 (+)
Sequence length	978
RNA-Seq Expression	Lsi03G002120.1
Synteny	Lsi03G002120.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCAACGTTTTGGCACAATATTCGATAAGATTTTGCTGCCACGTCCGTTTTCTTATGAATAACACATTGCCACGTGGTTATTTGATGGCTTTATTTCTTCAATAGTTTTTCCATCTAAATCTTTTTCTTGTGGAAAAGAAAAGTAGACCAAGCAACTAAAATAAATGAAAGGAAGGGAAATGGTAATAATCTTGAAAAATAGTAATAAAATCCAATCTAATTTTTTAAAATAAGGTTTATAAACACTATTTCACCCATAATTCTTCGTATGTTTTGATTGATTGTGACATATTTTGAACACTTTTTTAATTTGACAACTGATAAAAGTAATGTTGTATTTGATTGATAGGCAAGTTTCGTGGTGACTAAAGTGAGTGCAATTTGTCCATTGAGATTACTGGAGAGAGTTAGCGACTTATTAAGATGGCAACAAAAGAACCCTAGTTTCAATCTTCCATGGAAGCTCGAAACTTTGCCAATTTTCTCAGAATCAAGCCCTCTTTACCACACTCTTGAACAACCAAAACCCTTAACAAATGAAGAAGAAAAAAGCCTCCAAATGAGCCATGAAAGGCTAATGAAAATTTGTGAAAGTTGTGTTGATGCCAATATTCCTTTAGCCATTGATGCTGAACATACCAAAGTTCAACCAGCCATTGATTACTTCACTTATTCAGCTGCCATTATTCACAACAAAGACCACAATCCAATAGTTTATGGAACAATTCAAGCCTATCTAAAAGATGCAAAAGATAGACTTTTGTTAGTTACCAAAGAAGCTACCAAAATGAAAGTTCCACTTGGTATTAAGCTTGTGAGAGGTGCTTATATGTCAAGTGAATCCAAACTTGCTTCTTCTCTTGGCTTTGAATCTCCAATTCACAATACAATTCAAGATACACATTTTTGCTACAATACTTGTGCATCTTTCTTGCTTGACAACATCGCCAAAGGCTCGAGTGGAGCTATTCTTGCAACCCATAACATCGAATCAGGTAATGAATTATGCTCTATGCTCTATGCTCTTCTTATTTCCAAACCGACCCAGTTGTTTGATAACCATTTCGGTTTCTTTTGAATAGGGAAATTGGCAGCTAGTAAAGCATATGAGATAGGGATAGGAAAGTTGAAGCAAAAGCTTGAGTTTGCTCAATTGTATGGAATGTCAGAAGCACTTTCTTTTGGGTTGAGAAATGCAGGGTTTCAAGTGAGTAAATACATGCCATTTGGACCTGTGGATATGGTAATGCCATACCTTTTAAGAAGAGCTGAAGAGAATAGAGGTCTCTTATCAACTTCAAATTTGGATAGACAACTTATGAGGTAATTCAAAACATATTTAATTAATTTTCATATAGTATGTTGAAAATTTTGTTAAACTATTTACAGAAAAATAACAAAAAAAAAAAAAAAAAACACGGATAGACATTATCAACTTCTATCAGCATCTATCAGTGATAAAACTTCTATCATTTCTATCCGCGTCTATCACAACTATCTAAACATTTTACTATTTTGTGTAAATAGTTTTTTTTATTTTTTTATTTTTAAAAATTCTCTTAGTATATATATGGATTTGACACATTTGAAATTTCTTGATTCTTTGCAGAAGGGAGATGGGAAGGAGGATGAAAGAGTACTTTAGGTGAAAAGAAAGGGCCTTTTAGCAATTTCAACAACCTACAACACCAAGATTTGCAATTTTTGTTTATAGGATCACACATGGTTTTAAAAACCTACTTTGTTTTGTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTAAAATCAAACAAAAGTTAAATTGCAAAACCACTTTGGTGAACAAAAAATATAATGTTTACTTAAAATATATATGTGGTTAAGTTACAAATTTATAATTCTTTTTTAGGATAAAAATTATGATGTTGGAAATCAAATTTAGAACTGTTTTCAAATATAAAAAAATGAGTTAAATTATTTACGAATATAGAAATTTTTTACTACATATTAAGGATAAACCGATTATAAAACTTGAACAATAAATAAGAATAGTCAAGTCAGTGAAATTTTTTAATATTTATAAATAATCTGATTTTTTTTGTTTAACAGTAATTTCTCAAAAAAAGTAAAAAAAGCGGTTGTGAGTTCAATCTATAGTGGCCATATTTAATATCCTACGATAAGTTGGCAGGCTGCCTGTTATAGGGTCGGGTGGATTGTCTCGTGAGATTAATAGAGATGGGCGTAAGTTGTCCAGACACTCACGGATATAAAAAAAGAAAAAACACATTAATTTCCCACAAAAACACAGTAATTTCCCACAAAATTAAGTATATTTATCATAAATAATTTAATTCTTAATTTAACTGAAAAAATATTCTCACCCACTCTTCTTCCCAACCCGTCTGAAATCGGACGCTCTCTCTGCTCTCTCTCCCTCCTTAACTTACCGCCGGCCGCCTTCTCTCTCCGTCTCCGGCATTCAGGGATCAGTTTCTCCTGTCTCTCAAGAAACCGATCGCTTCACAGAATACAGCAACCAACAGTAAGTCCTACAAATAGAATAATCGGAAGCATTTTTTAATAATTTTACTTCAATTGAGAGTATGATTGGAGGCTGTTTTTGGATCCTTTAAGTGTCATCTTACTTGTTCAATTGATCAATTCAACCCTATCTTGTGTATCGTATTCGTATTCTACGCCTCAATTTGGAGATTATATATATAATTTTCTTTTATTTATTCAACACTATCAGTGAATTAGATTCTCGAGAATCTCCTCTTTTGGCTTTGGAGTTTTTAACATTTCCTCTGTCGACACTGAGGCAGAAAGCAGAATAAAGTGGCAATGCGGAAAGTGCTCGTAACTTGTTATAGATCATCCATATCAGAATTTTTCAATTCCGTTTTCTTCTGGGTTCAAATTCAAACCTACCTGCGAATTTAATTTATGATGGTGGTGCAGAAGCCCTAGAGGTACGAAATTCCTTTGCTATCTTCAACGATCTCAATCAAATATACAAAGATATAGGGTTTGCGAAATTGTTATTCTTTACCTTTCTGTTATGGGATGTCGAGGTTGAATTTGCTTAGAAGCGATGATATGTGCTTCATTCGATGAAATTCGTTTCCCAAAACATGGATGTTTCGAAGAAAATCCATTAATTTTTCCTTAAATAAGTATTATTGAGCTTCTGCATCATTGACAGGCTGCGTCTTTCTTTTTAGGGATTTCTGGGTAG

mRNA sequence

ATGGTCAACGTTTTGGCACAATATTCGATAAGATTTTGCTGCCACGCAAGTTTCGTGGTGACTAAAGTGAGTGCAATTTGTCCATTGAGATTACTGGAGAGAGTTAGCGACTTATTAAGATGGCAACAAAAGAACCCTAGTTTCAATCTTCCATGGAAGCTCGAAACTTTGCCAATTTTCTCAGAATCAAGCCCTCTTTACCACACTCTTGAACAACCAAAACCCTTAACAAATGAAGAAGAAAAAAGCCTCCAAATGAGCCATGAAAGGCTAATGAAAATTTGTGAAAGTTGTGTTGATGCCAATATTCCTTTAGCCATTGATGCTGAACATACCAAAGTTCAACCAGCCATTGATTACTTCACTTATTCAGCTGCCATTATTCACAACAAAGACCACAATCCAATAGTTTATGGAACAATTCAAGCCTATCTAAAAGATGCAAAAGATAGACTTTTGTTAGTTACCAAAGAAGCTACCAAAATGAAAGTTCCACTTGGTATTAAGCTTGTGAGAGGTGCTTATATGTCAAGTGAATCCAAACTTGCTTCTTCTCTTGGCTTTGAATCTCCAATTCACAATACAATTCAAGATACACATTTTTGCTACAATACTTGTGCATCTTTCTTGCTTGACAACATCGCCAAAGGCTCGAGTGGAGCTATTCTTGCAACCCATAACATCGAATCAGGTAATGAATTATGCTCTATGCTCTATGCTCTTCTTATTTCCAAACCGACCCAGTTGTTTGATAACCATTTCGGGTTTCAAGTGAGTAAATACATGCCATTTGGACCTGTGGATATGGTAATGCCATACCTTTTAAGAAGAGCTGAAGAGAATAGAGGGTCGGGTGGATTGTCTCGTGAGATTAATAGAGATGGGCGGATCAGTTTCTCCTGTCTCTCAAGAAACCGATCGCTTCACAGAATACAGCAACCAACAGCTGCGTCTTTCTTTTTAGGGATTTCTGGGTAG

Coding sequence (CDS)

ATGGTCAACGTTTTGGCACAATATTCGATAAGATTTTGCTGCCACGCAAGTTTCGTGGTGACTAAAGTGAGTGCAATTTGTCCATTGAGATTACTGGAGAGAGTTAGCGACTTATTAAGATGGCAACAAAAGAACCCTAGTTTCAATCTTCCATGGAAGCTCGAAACTTTGCCAATTTTCTCAGAATCAAGCCCTCTTTACCACACTCTTGAACAACCAAAACCCTTAACAAATGAAGAAGAAAAAAGCCTCCAAATGAGCCATGAAAGGCTAATGAAAATTTGTGAAAGTTGTGTTGATGCCAATATTCCTTTAGCCATTGATGCTGAACATACCAAAGTTCAACCAGCCATTGATTACTTCACTTATTCAGCTGCCATTATTCACAACAAAGACCACAATCCAATAGTTTATGGAACAATTCAAGCCTATCTAAAAGATGCAAAAGATAGACTTTTGTTAGTTACCAAAGAAGCTACCAAAATGAAAGTTCCACTTGGTATTAAGCTTGTGAGAGGTGCTTATATGTCAAGTGAATCCAAACTTGCTTCTTCTCTTGGCTTTGAATCTCCAATTCACAATACAATTCAAGATACACATTTTTGCTACAATACTTGTGCATCTTTCTTGCTTGACAACATCGCCAAAGGCTCGAGTGGAGCTATTCTTGCAACCCATAACATCGAATCAGGTAATGAATTATGCTCTATGCTCTATGCTCTTCTTATTTCCAAACCGACCCAGTTGTTTGATAACCATTTCGGGTTTCAAGTGAGTAAATACATGCCATTTGGACCTGTGGATATGGTAATGCCATACCTTTTAAGAAGAGCTGAAGAGAATAGAGGGTCGGGTGGATTGTCTCGTGAGATTAATAGAGATGGGCGGATCAGTTTCTCCTGTCTCTCAAGAAACCGATCGCTTCACAGAATACAGCAACCAACAGCTGCGTCTTTCTTTTTAGGGATTTCTGGGTAG

Protein sequence

MVNVLAQYSIRFCCHASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKPLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTIQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFDNHFGFQVSKYMPFGPVDMVMPYLLRRAEENRGSGGLSREINRDGRISFSCLSRNRSLHRIQQPTAASFFLGISG

Homology

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BLAST of Lsi03G002120.1 vs. ExPASy Swiss-Prot
Match: Q6NKX1 (Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.5e-85
Identity = 165/299 (55.18%), Postives = 208/299 (69.57%), Query Frame = 0


Query: 15  HASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPK 74
           H S VV K++AICP+ +L+RVSDLLRWQ KNP+F LPWKL + P+FS  SPLYHT  +P+
Sbjct: 168 HLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYHTTSEPE 227

Query: 75  PLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN 134
           PLT EEE+ L+ +HERL  +C  C ++N+PL IDAE T +QPAIDY  Y +AI+ N D +
Sbjct: 228 PLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIMFNSDKD 287

Query: 135 -PIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIH 194
            PIVY TIQAYLKDA +RL L  +E+ KM VP+G KLVRGAYMSSE+KLA SLG++SP+H
Sbjct: 288 RPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLGYKSPVH 347

Query: 195 NTIQDTHFCYNTCASFLLDNIAKGSS-GAILATHNIESGNELCSMLYALLISKP------ 254
           +TIQ+TH CYN C SFL++  + GS    ILATHN +SG         L I+K       
Sbjct: 348 DTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKENGKIEF 407

Query: 255 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
            QL+             GF VSKYMP+GPVD  +PYL+RRA ENRG   +G L R++ R
Sbjct: 408 AQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALDRQLMR 466

[Close]

BLAST of Lsi03G002120.1 vs. ExPASy Swiss-Prot
Match: P92983 (Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 PE=1 SV=2)

HSP 1 Score: 307.4 bits (786), Expect = 2.0e-82
Identity = 162/299 (54.18%), Postives = 205/299 (68.56%), Query Frame = 0


Query: 15  HASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPK 74
           H S VV K++AICP+ LL+RVSDLLRW+ K+P+F L WKL++ P+FSESSPLYHT  +P+
Sbjct: 189 HFSSVVVKITAICPISLLKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPE 248

Query: 75  PLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN 134
           PLT EEE+ L+ +H R+ +IC  C ++N+PL IDAE T +QPAIDY  YS+AI+ N D +
Sbjct: 249 PLTAEEERELEAAHGRIQEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKD 308

Query: 135 -PIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIH 194
            PIVY TIQAYL+DA +RL L  + A K  VP+G KLVRGAYMSSE+ LA SLG +SP+H
Sbjct: 309 RPIVYNTIQAYLRDAGERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVH 368

Query: 195 NTIQDTHFCYNTCASFLLDNIAKGSS-GAILATHNIESGNELCSMLYALLISKP------ 254
           +TIQDTH CYN C +FL++  + GS  G +LATHN +SG         L I K       
Sbjct: 369 DTIQDTHSCYNDCMTFLMEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEF 428

Query: 255 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
            QL+             GF VSKYMPFGPV   +PYLLRRA ENRG   +G   R++ R
Sbjct: 429 AQLYGMSDALSFGLKRAGFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMR 487

[Close]

BLAST of Lsi03G002120.1 vs. ExPASy Swiss-Prot
Match: Q86H28 (Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=prodh PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.7e-31
Identity = 99/295 (33.56%), Postives = 150/295 (50.85%), Query Frame = 0


Query: 17  SFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP- 76
           SF   K + +    +LE+++ L+         N+   +  LPI S +SPL   L Q    
Sbjct: 265 SFSCVKFTGLVTPSVLEKMNTLVS--------NVTTNVSELPIESFNSPLDFYLNQSSSL 324

Query: 77  --------LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAI 136
                   LT+++ K ++    R+ KI + C    +P+ +DAE +  Q AI + T S +I
Sbjct: 325 MKQGSEPLLTSKDIKEIKEFFNRMDKIFQLCHQRGVPILVDAEQSYYQVAIHHLTMSYSI 384

Query: 137 IHNKDHNPIVYGTIQAYLKDAKDRLL--LVTKEATKMKVPLGIKLVRGAYMSSESKLASS 196
            +NK+  PI+Y T Q YL +  + L        + K    LG K+VRGAYM +ES+ +  
Sbjct: 385 KYNKE-KPIIYNTYQMYLVNGMNVLKQHFELSSSQKFNFKLGAKIVRGAYMVTESERSQR 444

Query: 197 LGFESPIHNTIQDTHFCYNTCASFLLDNIAK--GSSGAILATHNIESGNELCSML--YAL 256
           L  E+P+  TIQDTH  YNT   FLL+ I     S G ++A+HN +S N    ++  Y +
Sbjct: 445 LSTENPVLPTIQDTHKSYNTALDFLLNQIKSDPNSIGLMIASHNEDSINLGTKLIKQYKI 504

Query: 257 LISKPTQLFDNHFGF-------------QVSKYMPFGPVDMVMPYLLRRAEENRG 284
             + P   F   FG              ++ KY+PFGPV+ V+PYL+RR  EN+G
Sbjct: 505 DPTNPNIQFGQLFGMADFLSFNLVDQHQRIFKYVPFGPVEEVLPYLIRRMHENKG 550

[Close]

BLAST of Lsi03G002120.1 vs. ExPASy Swiss-Prot
Match: Q9WU79 (Proline dehydrogenase 1, mitochondrial OS=Mus musculus OX=10090 GN=Prodh PE=1 SV=2)

HSP 1 Score: 114.8 bits (286), Expect = 1.9e-24
Identity = 75/221 (33.94%), Postives = 116/221 (52.49%), Query Frame = 0


Query: 77  TNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPI 136
           T EEE+ ++   +R+  + +   +A + L IDAE +  QPAI   T       N D  P 
Sbjct: 348 TEEEEQQMKRMLQRMDVLAKKAKEAGVRLMIDAEQSYFQPAISRLTLEMQRRFNVD-KPF 407

Query: 137 VYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTI 196
           ++ T Q YLKDA D + L  + A +     G KLVRGAYM+ E   A+ +G+E PI+ T 
Sbjct: 408 IFNTFQCYLKDAYDNVTLDMELARREGWCFGAKLVRGAYMAQERVRAAEIGYEDPINPTY 467

Query: 197 QDTHFCYNTCASFLLDNIAKGSSGAIL-ATHNIESGN-ELCSM------------LYALL 256
           + T+  Y+ C +++L+ +   +   ++ A+HN ++ +  LC M             +  L
Sbjct: 468 EATNAMYHRCLNYVLEELKHSTKAEVMVASHNEDTVHFTLCRMKEIGLHPADGQVCFGQL 527

Query: 257 ISKPTQLFD--NHFGFQVSKYMPFGPVDMVMPYLLRRAEEN 282
           +    Q+       GF V KY+P+GPV  V+PYL RRA EN
Sbjct: 528 LGMCDQISFPLGQAGFPVYKYVPYGPVMEVLPYLSRRALEN 567

[Close]

BLAST of Lsi03G002120.1 vs. ExPASy Swiss-Prot
Match: O45228 (Proline dehydrogenase 1, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=prdh-1 PE=2 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 1.2e-23
Identity = 77/261 (29.50%), Postives = 128/261 (49.04%), Query Frame = 0


Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           L+NEEE+  +    R + + E  ++  + + +DAE T +QPAI   T      +NK    
Sbjct: 359 LSNEEEQEFRNMVRRTLDVAEYAIEKGVRIMVDAEQTYLQPAISKITIEMMKKYNKGRGN 418

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           I + T QAYLK     +    + A +     G KLVRGAYM  E   A ++G+E PI++ 
Sbjct: 419 I-FNTYQAYLKGTLQNMEADMQVARREGWHFGAKLVRGAYMEQERARAKAIGYEDPINDN 478

Query: 196 IQDTHFCYNTCASFLLDNI---AKGSSGAILATHNIESGNELCSMLYALLISKP------ 255
            + T   Y +C + + D +    K +   ++A+HN ++     +++    IS        
Sbjct: 479 FEATSKMYESCLTRIADEVHRRGKTNVSVMVASHNEDTVRFALNLMKEKCISPSERVMCM 538

Query: 256 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRGSGGLSREINRDGRI 315
            QL+             GF V KY+P+GPV+ V+PYL RRA EN   G + ++ N++  +
Sbjct: 539 AQLYGMCDQVSFSLGQAGFSVYKYLPYGPVEEVLPYLSRRALEN---GSVLKKANKERDL 598

Query: 316 SFSCLSRNRSLHRIQQPTAAS 319
            +  L R  S    +  +++S
Sbjct: 599 LWKELKRRISSGEFKARSSSS 615

[Close]

BLAST of Lsi03G002120.1 vs. ExPASy TrEMBL
Match: A0A0A0KXN4 (Proline dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G643300 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.5e-127
Identity = 239/296 (80.74%), Postives = 254/296 (85.81%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 185 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 244

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQMSHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 245 LTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 304

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+KVPLGIKLVRGAYMSSESKLASSLGFESPIH+T
Sbjct: 305 IVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESPIHDT 364

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGS+GAILATHN+ESG    S  Y + I K  Q  +    
Sbjct: 365 IQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLEFAQL 424

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L RE+ R
Sbjct: 425 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480

[Close]

BLAST of Lsi03G002120.1 vs. ExPASy TrEMBL
Match: A0A1S3CBE9 (Proline dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498577 PE=3 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 7.9e-127
Identity = 237/296 (80.07%), Postives = 254/296 (85.81%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 179 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 238

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQ+SHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 239 LTREEEKSLQISHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 298

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+K+PLGIKLVRGAYMSSESKLASSLGFESPIHNT
Sbjct: 299 IVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPIHNT 358

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGSSGAILATHN+ESG    +  Y + I K  Q  +    
Sbjct: 359 IQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEFAQL 418

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 419 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 474

[Close]

BLAST of Lsi03G002120.1 vs. ExPASy TrEMBL
Match: A0A5D3BNA9 (Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002050 PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 2.3e-126
Identity = 236/296 (79.73%), Postives = 253/296 (85.47%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 179 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 238

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQ+SHERLMKIC+SCVD N+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 239 LTREEEKSLQISHERLMKICQSCVDTNVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 298

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+K+PLGIKLVRGAYMSSESKLASSLGFESPIHNT
Sbjct: 299 IVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPIHNT 358

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGSSGAILATHN+ESG    +  Y + I K  Q  +    
Sbjct: 359 IQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEFAQL 418

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 419 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 474

[Close]

BLAST of Lsi03G002120.1 vs. ExPASy TrEMBL
Match: A0A6J1JZR8 (Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489341 PE=3 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 4.5e-122
Identity = 227/296 (76.69%), Postives = 249/296 (84.12%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVV KVSAICPLRLLERVSDLLRWQQK+P  NLPWKL+T PIFSESSPLYHTL+QP P
Sbjct: 178 ASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQPNP 237

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LTNEE+KSLQ+SH+RLMKIC+SCVDAN+PLAIDAEHTK+QPAIDYFTYSAAIIHNKDHNP
Sbjct: 238 LTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHNP 297

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGT+QAYLKDAKDRLLLVTKEA KM+VPLGIKLVRGAYMSSESKLASSLGFESPIHN+
Sbjct: 298 IVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNS 357

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           I DTH CYN+CASFLLDNIA GSSGAILATHN+ESG  +    Y + I K  +  +    
Sbjct: 358 IHDTHACYNSCASFLLDNIAAGSSGAILATHNVESGKLVAKKAYEIGIEKLKEKLEFAQL 417

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFG VDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 418 YGMSEALSYGLRNAGFQVSKYMPFGGVDMVMPYLLRRAEENRGLLSASNLDRQLMR 473

[Close]

BLAST of Lsi03G002120.1 vs. ExPASy TrEMBL
Match: A0A6J1FGN7 (Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 2.2e-121
Identity = 228/296 (77.03%), Postives = 249/296 (84.12%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVV KVSAICPLRLLERVSDLLRWQQK+P  NLPWKL+T PIFSESSPLYHTL+QP P
Sbjct: 184 ASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQPNP 243

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LTNEE+KSLQ+SH+RLMKIC+SCVDAN+PLAIDAEHTK+QPAIDYFTYSAAIIHNKDHNP
Sbjct: 244 LTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHNP 303

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGT+QAYLKDAKDRLLLVTKEA +M+VPLGIKLVRGAYMSSESKLASSLGFESPIHN+
Sbjct: 304 IVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNS 363

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKP------TQL 255
           I DTH CYN+CASFLLDNIA GSSGAILATHN+ESG       Y + I K        QL
Sbjct: 364 IHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEFAQL 423

Query: 256 FD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
           +           + GFQVSKYMPFG VDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 424 YGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMR 479

[Close]

BLAST of Lsi03G002120.1 vs. NCBI nr
Match: XP_038891036.1 (proline dehydrogenase 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 468.0 bits (1203), Expect = 6.6e-128
Identity = 243/307 (79.15%), Postives = 259/307 (84.36%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQPKP
Sbjct: 183 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTLEQPKP 242

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT+EEEKSLQMSHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKDH P
Sbjct: 243 LTHEEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHIP 302

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLLV KEA+K+KVPLGIKLVRGAYMSSESK+ASSLGFESPIHNT
Sbjct: 303 IVYGTIQAYLKDAKDRLLLVNKEASKLKVPLGIKLVRGAYMSSESKIASSLGFESPIHNT 362

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLDNIAKGSSG ILATHN+ESG    +  Y + + K  Q  +    
Sbjct: 363 IQDTHVCYNTCASFLLDNIAKGSSGVILATHNVESGKLAATKAYEIGMGKLKQKLEFAQL 422

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINRD--G 303
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L R++ R   G
Sbjct: 423 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQLMRKELG 482

[Close]

BLAST of Lsi03G002120.1 vs. NCBI nr
Match: XP_004141603.2 (proline dehydrogenase 2, mitochondrial [Cucumis sativus] >KGN52551.1 hypothetical protein Csa_008665 [Cucumis sativus])

HSP 1 Score: 464.5 bits (1194), Expect = 7.3e-127
Identity = 239/296 (80.74%), Postives = 254/296 (85.81%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 185 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 244

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQMSHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 245 LTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 304

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+KVPLGIKLVRGAYMSSESKLASSLGFESPIH+T
Sbjct: 305 IVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESPIHDT 364

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGS+GAILATHN+ESG    S  Y + I K  Q  +    
Sbjct: 365 IQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLEFAQL 424

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L RE+ R
Sbjct: 425 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480

[Close]

BLAST of Lsi03G002120.1 vs. NCBI nr
Match: XP_008459446.1 (PREDICTED: proline dehydrogenase 2, mitochondrial [Cucumis melo])

HSP 1 Score: 463.4 bits (1191), Expect = 1.6e-126
Identity = 237/296 (80.07%), Postives = 254/296 (85.81%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 179 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 238

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQ+SHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 239 LTREEEKSLQISHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 298

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+K+PLGIKLVRGAYMSSESKLASSLGFESPIHNT
Sbjct: 299 IVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPIHNT 358

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGSSGAILATHN+ESG    +  Y + I K  Q  +    
Sbjct: 359 IQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEFAQL 418

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 419 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 474

[Close]

BLAST of Lsi03G002120.1 vs. NCBI nr
Match: KAA0039400.1 (proline dehydrogenase 2 [Cucumis melo var. makuwa] >TYK00588.1 proline dehydrogenase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 461.8 bits (1187), Expect = 4.7e-126
Identity = 236/296 (79.73%), Postives = 253/296 (85.47%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 179 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 238

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQ+SHERLMKIC+SCVD N+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 239 LTREEEKSLQISHERLMKICQSCVDTNVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 298

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+K+PLGIKLVRGAYMSSESKLASSLGFESPIHNT
Sbjct: 299 IVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPIHNT 358

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGSSGAILATHN+ESG    +  Y + I K  Q  +    
Sbjct: 359 IQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEFAQL 418

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 419 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 474

[Close]

BLAST of Lsi03G002120.1 vs. NCBI nr
Match: XP_022993279.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 447.6 bits (1150), Expect = 9.3e-122
Identity = 227/296 (76.69%), Postives = 249/296 (84.12%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVV KVSAICPLRLLERVSDLLRWQQK+P  NLPWKL+T PIFSESSPLYHTL+QP P
Sbjct: 178 ASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQPNP 237

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LTNEE+KSLQ+SH+RLMKIC+SCVDAN+PLAIDAEHTK+QPAIDYFTYSAAIIHNKDHNP
Sbjct: 238 LTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHNP 297

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGT+QAYLKDAKDRLLLVTKEA KM+VPLGIKLVRGAYMSSESKLASSLGFESPIHN+
Sbjct: 298 IVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNS 357

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           I DTH CYN+CASFLLDNIA GSSGAILATHN+ESG  +    Y + I K  +  +    
Sbjct: 358 IHDTHACYNSCASFLLDNIAAGSSGAILATHNVESGKLVAKKAYEIGIEKLKEKLEFAQL 417

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFG VDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 418 YGMSEALSYGLRNAGFQVSKYMPFGGVDMVMPYLLRRAEENRGLLSASNLDRQLMR 473

[Close]

BLAST of Lsi03G002120.1 vs. TAIR 10
Match: AT5G38710.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 315.8 bits (808), Expect = 3.9e-86
Identity = 165/299 (55.18%), Postives = 208/299 (69.57%), Query Frame = 0


Query: 15  HASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPK 74
           H S VV K++AICP+ +L+RVSDLLRWQ KNP+F LPWKL + P+FS  SPLYHT  +P+
Sbjct: 168 HLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYHTTSEPE 227

Query: 75  PLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN 134
           PLT EEE+ L+ +HERL  +C  C ++N+PL IDAE T +QPAIDY  Y +AI+ N D +
Sbjct: 228 PLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIMFNSDKD 287

Query: 135 -PIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIH 194
            PIVY TIQAYLKDA +RL L  +E+ KM VP+G KLVRGAYMSSE+KLA SLG++SP+H
Sbjct: 288 RPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLGYKSPVH 347

Query: 195 NTIQDTHFCYNTCASFLLDNIAKGSS-GAILATHNIESGNELCSMLYALLISKP------ 254
           +TIQ+TH CYN C SFL++  + GS    ILATHN +SG         L I+K       
Sbjct: 348 DTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKENGKIEF 407

Query: 255 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
            QL+             GF VSKYMP+GPVD  +PYL+RRA ENRG   +G L R++ R
Sbjct: 408 AQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALDRQLMR 466

[Close]

BLAST of Lsi03G002120.1 vs. TAIR 10
Match: AT3G30775.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.4e-83
Identity = 162/299 (54.18%), Postives = 205/299 (68.56%), Query Frame = 0


Query: 15  HASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPK 74
           H S VV K++AICP+ LL+RVSDLLRW+ K+P+F L WKL++ P+FSESSPLYHT  +P+
Sbjct: 189 HFSSVVVKITAICPISLLKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPE 248

Query: 75  PLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN 134
           PLT EEE+ L+ +H R+ +IC  C ++N+PL IDAE T +QPAIDY  YS+AI+ N D +
Sbjct: 249 PLTAEEERELEAAHGRIQEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKD 308

Query: 135 -PIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIH 194
            PIVY TIQAYL+DA +RL L  + A K  VP+G KLVRGAYMSSE+ LA SLG +SP+H
Sbjct: 309 RPIVYNTIQAYLRDAGERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVH 368

Query: 195 NTIQDTHFCYNTCASFLLDNIAKGSS-GAILATHNIESGNELCSMLYALLISKP------ 254
           +TIQDTH CYN C +FL++  + GS  G +LATHN +SG         L I K       
Sbjct: 369 DTIQDTHSCYNDCMTFLMEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEF 428

Query: 255 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
            QL+             GF VSKYMPFGPV   +PYLLRRA ENRG   +G   R++ R
Sbjct: 429 AQLYGMSDALSFGLKRAGFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMR 487

The following BLAST results are available for this feature:

BLAST of Lsi03G002120.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NKX1	5.5e-85	55.18	Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 P...	[more]
P92983	2.0e-82	54.18	Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 P...	[more]
Q86H28	2.7e-31	33.56	Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=p...	[more]
Q9WU79	1.9e-24	33.94	Proline dehydrogenase 1, mitochondrial OS=Mus musculus OX=10090 GN=Prodh PE=1 SV...	[more]
O45228	1.2e-23	29.50	Proline dehydrogenase 1, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=prdh...	[more]

BLAST of Lsi03G002120.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXN4	3.5e-127	80.74	Proline dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G643300 PE=3 SV=1	[more]
A0A1S3CBE9	7.9e-127	80.07	Proline dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498577 PE=3 SV=1	[more]
A0A5D3BNA9	2.3e-126	79.73	Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16...	[more]
A0A6J1JZR8	4.5e-122	76.69	Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489341 PE=3 SV=1	[more]
A0A6J1FGN7	2.2e-121	77.03	Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1	[more]

BLAST of Lsi03G002120.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891036.1	6.6e-128	79.15	proline dehydrogenase 2, mitochondrial-like [Benincasa hispida]	[more]
XP_004141603.2	7.3e-127	80.74	proline dehydrogenase 2, mitochondrial [Cucumis sativus] >KGN52551.1 hypothetica...	[more]
XP_008459446.1	1.6e-126	80.07	PREDICTED: proline dehydrogenase 2, mitochondrial [Cucumis melo]	[more]
KAA0039400.1	4.7e-126	79.73	proline dehydrogenase 2 [Cucumis melo var. makuwa] >TYK00588.1 proline dehydroge...	[more]
XP_022993279.1	9.3e-122	76.69	proline dehydrogenase 2, mitochondrial-like [Cucurbita maxima]	[more]

BLAST of Lsi03G002120.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G38710.1	3.9e-86	55.18	Methylenetetrahydrofolate reductase family protein	[more]
AT3G30775.1	1.4e-83	54.18	Methylenetetrahydrofolate reductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002872	Proline dehydrogenase domain	PFAM	PF01619	Pro_dh	coord: 70..284 e-value: 4.1E-51 score: 174.1
None	No IPR available	GENE3D	3.20.20.220		coord: 60..315 e-value: 6.8E-58 score: 198.5
None	No IPR available	PANTHER	PTHR13914:SF25	PROLINE DEHYDROGENASE	coord: 16..284
IPR015659	Proline oxidase family	PANTHER	PTHR13914	PROLINE OXIDASE	coord: 16..284
IPR029041	FAD-linked oxidoreductase-like	SUPERFAMILY	51730	FAD-linked oxidoreductase	coord: 14..289

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi03G002120	Lsi03G002120	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi03G002120.1.exon.1	Lsi03G002120.1.exon.1	exon
Lsi03G002120.1.exon.2	Lsi03G002120.1.exon.2	exon
Lsi03G002120.1.exon.3	Lsi03G002120.1.exon.3	exon
Lsi03G002120.1.exon.4	Lsi03G002120.1.exon.4	exon
Lsi03G002120.1.exon.5	Lsi03G002120.1.exon.5	exon
Lsi03G002120.1.exon.6	Lsi03G002120.1.exon.6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi03G002120.1.CDS.1	Lsi03G002120.1.CDS.1	CDS
Lsi03G002120.1.CDS.2	Lsi03G002120.1.CDS.2	CDS
Lsi03G002120.1.CDS.3	Lsi03G002120.1.CDS.3	CDS
Lsi03G002120.1.CDS.4	Lsi03G002120.1.CDS.4	CDS
Lsi03G002120.1.CDS.5	Lsi03G002120.1.CDS.5	CDS
Lsi03G002120.1.CDS.6	Lsi03G002120.1.CDS.6	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi03G002120.1	Lsi03G002120.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006562	proline catabolic process
biological_process	GO:0010133	proline catabolic process to glutamate
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004657	proline dehydrogenase activity
molecular_function	GO:0071949	FAD binding