Lsi03G002120 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G002120
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Proline dehydrogenase
Location	chr03: 2724191 .. 2728268 (+)
RNA-Seq Expression	Lsi03G002120
Synteny	Lsi03G002120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCAACGTTTTGGCACAATATTCGATAAGATTTTGCTGCCACGTCCGTTTTCTTATGAATAACACATTGCCACGTGGTTATTTGATGGCTTTATTTCTTCAATAGTTTTTCCATCTAAATCTTTTTCTTGTGGAAAAGAAAAGTAGACCAAGCAACTAAAATAAATGAAAGGAAGGGAAATGGTAATAATCTTGAAAAATAGTAATAAAATCCAATCTAATTTTTTAAAATAAGGTTTATAAACACTATTTCACCCATAATTCTTCGTATGTTTTGATTGATTGTGACATATTTTGAACACTTTTTTAATTTGACAACTGATAAAAGTAATGTTGTATTTGATTGATAGGCAAGTTTCGTGGTGACTAAAGTGAGTGCAATTTGTCCATTGAGATTACTGGAGAGAGTTAGCGACTTATTAAGATGGCAACAAAAGAACCCTAGTTTCAATCTTCCATGGAAGCTCGAAACTTTGCCAATTTTCTCAGAATCAAGCCCTCTTTACCACACTCTTGAACAACCAAAACCCTTAACAAATGAAGAAGAAAAAAGCCTCCAAATGAGCCATGAAAGGCTAATGAAAATTTGTGAAAGTTGTGTTGATGCCAATATTCCTTTAGCCATTGATGCTGAACATACCAAAGTTCAACCAGCCATTGATTACTTCACTTATTCAGCTGCCATTATTCACAACAAAGACCACAATCCAATAGTTTATGGAACAATTCAAGCCTATCTAAAAGATGCAAAAGATAGACTTTTGTTAGTTACCAAAGAAGCTACCAAAATGAAAGTTCCACTTGGTATTAAGCTTGTGAGAGGTGCTTATATGTCAAGTGAATCCAAACTTGCTTCTTCTCTTGGCTTTGAATCTCCAATTCACAATACAATTCAAGATACACATTTTTGCTACAATACTTGTGCATCTTTCTTGCTTGACAACATCGCCAAAGGCTCGAGTGGAGCTATTCTTGCAACCCATAACATCGAATCAGGTAATGAATTATGCTCTATGCTCTATGCTCTTCTTATTTCCAAACCGACCCAGTTGTTTGATAACCATTTCGGTTTCTTTTGAATAGGGAAATTGGCAGCTAGTAAAGCATATGAGATAGGGATAGGAAAGTTGAAGCAAAAGCTTGAGTTTGCTCAATTGTATGGAATGTCAGAAGCACTTTCTTTTGGGTTGAGAAATGCAGGGTTTCAAGTGAGTAAATACATGCCATTTGGACCTGTGGATATGGTAATGCCATACCTTTTAAGAAGAGCTGAAGAGAATAGAGGTCTCTTATCAACTTCAAATTTGGATAGACAACTTATGAGGTAATTCAAAACATATTTAATTAATTTTCATATAGTATGTTGAAAATTTTGTTAAACTATTTACAGAAAAATAACAAAAAAAAAAAAAAAAAACACGGATAGACATTATCAACTTCTATCAGCATCTATCAGTGATAAAACTTCTATCATTTCTATCCGCGTCTATCACAACTATCTAAACATTTTACTATTTTGTGTAAATAGTTTTTTTTATTTTTTTATTTTTAAAAATTCTCTTAGTATATATATGGATTTGACACATTTGAAATTTCTTGATTCTTTGCAGAAGGGAGATGGGAAGGAGGATGAAAGAGTACTTTAGGTGAAAAGAAAGGGCCTTTTAGCAATTTCAACAACCTACAACACCAAGATTTGCAATTTTTGTTTATAGGATCACACATGGTTTTAAAAACCTACTTTGTTTTGTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTAAAATCAAACAAAAGTTAAATTGCAAAACCACTTTGGTGAACAAAAAATATAATGTTTACTTAAAATATATATGTGGTTAAGTTACAAATTTATAATTCTTTTTTAGGATAAAAATTATGATGTTGGAAATCAAATTTAGAACTGTTTTCAAATATAAAAAAATGAGTTAAATTATTTACGAATATAGAAATTTTTTACTACATATTAAGGATAAACCGATTATAAAACTTGAACAATAAATAAGAATAGTCAAGTCAGTGAAATTTTTTAATATTTATAAATAATCTGATTTTTTTTGTTTAACAGTAATTTCTCAAAAAAAGTAAAAAAAGCGGTTGTGAGTTCAATCTATAGTGGCCATATTTAATATCCTACGATAAGTTGGCAGGCTGCCTGTTATAGGGTCGGGTGGATTGTCTCGTGAGATTAATAGAGATGGGCGTAAGTTGTCCAGACACTCACGGATATAAAAAAAGAAAAAACACATTAATTTCCCACAAAAACACAGTAATTTCCCACAAAATTAAGTATATTTATCATAAATAATTTAATTCTTAATTTAACTGAAAAAATATTCTCACCCACTCTTCTTCCCAACCCGTCTGAAATCGGACGCTCTCTCTGCTCTCTCTCCCTCCTTAACTTACCGCCGGCCGCCTTCTCTCTCCGTCTCCGGCATTCAGGGATCAGTTTCTCCTGTCTCTCAAGAAACCGATCGCTTCACAGAATACAGCAACCAACAGTAAGTCCTACAAATAGAATAATCGGAAGCATTTTTTAATAATTTTACTTCAATTGAGAGTATGATTGGAGGCTGTTTTTGGATCCTTTAAGTGTCATCTTACTTGTTCAATTGATCAATTCAACCCTATCTTGTGTATCGTATTCGTATTCTACGCCTCAATTTGGAGATTATATATATAATTTTCTTTTATTTATTCAACACTATCAGTGAATTAGATTCTCGAGAATCTCCTCTTTTGGCTTTGGAGTTTTTAACATTTCCTCTGTCGACACTGAGGCAGAAAGCAGAATAAAGTGGCAATGCGGAAAGTGCTCGTAACTTGTTATAGATCATCCATATCAGAATTTTTCAATTCCGTTTTCTTCTGGGTTCAAATTCAAACCTACCTGCGAATTTAATTTATGATGGTGGTGCAGAAGCCCTAGAGGTACGAAATTCCTTTGCTATCTTCAACGATCTCAATCAAATATACAAAGATATAGGGTTTGCGAAATTGTTATTCTTTACCTTTCTGTTATGGGATGTCGAGGTTGAATTTGCTTAGAAGCGATGATATGTGCTTCATTCGATGAAATTCGTTTCCCAAAACATGGATGTTTCGAAGAAAATCCATTAATTTTTCCTTAAATAAGTATTATTGAGCTTCTGCATCATTGACAGGCTGCGTCTTTCTTTTTAGGGATTTCTGGGTAG

mRNA sequence

ATGGTCAACGTTTTGGCACAATATTCGATAAGATTTTGCTGCCACGCAAGTTTCGTGGTGACTAAAGTGAGTGCAATTTGTCCATTGAGATTACTGGAGAGAGTTAGCGACTTATTAAGATGGCAACAAAAGAACCCTAGTTTCAATCTTCCATGGAAGCTCGAAACTTTGCCAATTTTCTCAGAATCAAGCCCTCTTTACCACACTCTTGAACAACCAAAACCCTTAACAAATGAAGAAGAAAAAAGCCTCCAAATGAGCCATGAAAGGCTAATGAAAATTTGTGAAAGTTGTGTTGATGCCAATATTCCTTTAGCCATTGATGCTGAACATACCAAAGTTCAACCAGCCATTGATTACTTCACTTATTCAGCTGCCATTATTCACAACAAAGACCACAATCCAATAGTTTATGGAACAATTCAAGCCTATCTAAAAGATGCAAAAGATAGACTTTTGTTAGTTACCAAAGAAGCTACCAAAATGAAAGTTCCACTTGGTATTAAGCTTGTGAGAGGTGCTTATATGTCAAGTGAATCCAAACTTGCTTCTTCTCTTGGCTTTGAATCTCCAATTCACAATACAATTCAAGATACACATTTTTGCTACAATACTTGTGCATCTTTCTTGCTTGACAACATCGCCAAAGGCTCGAGTGGAGCTATTCTTGCAACCCATAACATCGAATCAGGTAATGAATTATGCTCTATGCTCTATGCTCTTCTTATTTCCAAACCGACCCAGTTGTTTGATAACCATTTCGGGTTTCAAGTGAGTAAATACATGCCATTTGGACCTGTGGATATGGTAATGCCATACCTTTTAAGAAGAGCTGAAGAGAATAGAGGGTCGGGTGGATTGTCTCGTGAGATTAATAGAGATGGGCGGATCAGTTTCTCCTGTCTCTCAAGAAACCGATCGCTTCACAGAATACAGCAACCAACAGCTGCGTCTTTCTTTTTAGGGATTTCTGGGTAG

Coding sequence (CDS)

ATGGTCAACGTTTTGGCACAATATTCGATAAGATTTTGCTGCCACGCAAGTTTCGTGGTGACTAAAGTGAGTGCAATTTGTCCATTGAGATTACTGGAGAGAGTTAGCGACTTATTAAGATGGCAACAAAAGAACCCTAGTTTCAATCTTCCATGGAAGCTCGAAACTTTGCCAATTTTCTCAGAATCAAGCCCTCTTTACCACACTCTTGAACAACCAAAACCCTTAACAAATGAAGAAGAAAAAAGCCTCCAAATGAGCCATGAAAGGCTAATGAAAATTTGTGAAAGTTGTGTTGATGCCAATATTCCTTTAGCCATTGATGCTGAACATACCAAAGTTCAACCAGCCATTGATTACTTCACTTATTCAGCTGCCATTATTCACAACAAAGACCACAATCCAATAGTTTATGGAACAATTCAAGCCTATCTAAAAGATGCAAAAGATAGACTTTTGTTAGTTACCAAAGAAGCTACCAAAATGAAAGTTCCACTTGGTATTAAGCTTGTGAGAGGTGCTTATATGTCAAGTGAATCCAAACTTGCTTCTTCTCTTGGCTTTGAATCTCCAATTCACAATACAATTCAAGATACACATTTTTGCTACAATACTTGTGCATCTTTCTTGCTTGACAACATCGCCAAAGGCTCGAGTGGAGCTATTCTTGCAACCCATAACATCGAATCAGGTAATGAATTATGCTCTATGCTCTATGCTCTTCTTATTTCCAAACCGACCCAGTTGTTTGATAACCATTTCGGGTTTCAAGTGAGTAAATACATGCCATTTGGACCTGTGGATATGGTAATGCCATACCTTTTAAGAAGAGCTGAAGAGAATAGAGGGTCGGGTGGATTGTCTCGTGAGATTAATAGAGATGGGCGGATCAGTTTCTCCTGTCTCTCAAGAAACCGATCGCTTCACAGAATACAGCAACCAACAGCTGCGTCTTTCTTTTTAGGGATTTCTGGGTAG

Protein sequence

MVNVLAQYSIRFCCHASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKPLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTIQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFDNHFGFQVSKYMPFGPVDMVMPYLLRRAEENRGSGGLSREINRDGRISFSCLSRNRSLHRIQQPTAASFFLGISG

Homology

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BLAST of Lsi03G002120 vs. ExPASy Swiss-Prot
Match: Q6NKX1 (Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.5e-85
Identity = 165/299 (55.18%), Postives = 208/299 (69.57%), Query Frame = 0


Query: 15  HASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPK 74
           H S VV K++AICP+ +L+RVSDLLRWQ KNP+F LPWKL + P+FS  SPLYHT  +P+
Sbjct: 168 HLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYHTTSEPE 227

Query: 75  PLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN 134
           PLT EEE+ L+ +HERL  +C  C ++N+PL IDAE T +QPAIDY  Y +AI+ N D +
Sbjct: 228 PLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIMFNSDKD 287

Query: 135 -PIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIH 194
            PIVY TIQAYLKDA +RL L  +E+ KM VP+G KLVRGAYMSSE+KLA SLG++SP+H
Sbjct: 288 RPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLGYKSPVH 347

Query: 195 NTIQDTHFCYNTCASFLLDNIAKGSS-GAILATHNIESGNELCSMLYALLISKP------ 254
           +TIQ+TH CYN C SFL++  + GS    ILATHN +SG         L I+K       
Sbjct: 348 DTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKENGKIEF 407

Query: 255 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
            QL+             GF VSKYMP+GPVD  +PYL+RRA ENRG   +G L R++ R
Sbjct: 408 AQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALDRQLMR 466

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BLAST of Lsi03G002120 vs. ExPASy Swiss-Prot
Match: P92983 (Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 PE=1 SV=2)

HSP 1 Score: 307.4 bits (786), Expect = 2.0e-82
Identity = 162/299 (54.18%), Postives = 205/299 (68.56%), Query Frame = 0


Query: 15  HASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPK 74
           H S VV K++AICP+ LL+RVSDLLRW+ K+P+F L WKL++ P+FSESSPLYHT  +P+
Sbjct: 189 HFSSVVVKITAICPISLLKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPE 248

Query: 75  PLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN 134
           PLT EEE+ L+ +H R+ +IC  C ++N+PL IDAE T +QPAIDY  YS+AI+ N D +
Sbjct: 249 PLTAEEERELEAAHGRIQEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKD 308

Query: 135 -PIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIH 194
            PIVY TIQAYL+DA +RL L  + A K  VP+G KLVRGAYMSSE+ LA SLG +SP+H
Sbjct: 309 RPIVYNTIQAYLRDAGERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVH 368

Query: 195 NTIQDTHFCYNTCASFLLDNIAKGSS-GAILATHNIESGNELCSMLYALLISKP------ 254
           +TIQDTH CYN C +FL++  + GS  G +LATHN +SG         L I K       
Sbjct: 369 DTIQDTHSCYNDCMTFLMEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEF 428

Query: 255 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
            QL+             GF VSKYMPFGPV   +PYLLRRA ENRG   +G   R++ R
Sbjct: 429 AQLYGMSDALSFGLKRAGFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMR 487

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BLAST of Lsi03G002120 vs. ExPASy Swiss-Prot
Match: Q86H28 (Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=prodh PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.7e-31
Identity = 99/295 (33.56%), Postives = 150/295 (50.85%), Query Frame = 0


Query: 17  SFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP- 76
           SF   K + +    +LE+++ L+         N+   +  LPI S +SPL   L Q    
Sbjct: 265 SFSCVKFTGLVTPSVLEKMNTLVS--------NVTTNVSELPIESFNSPLDFYLNQSSSL 324

Query: 77  --------LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAI 136
                   LT+++ K ++    R+ KI + C    +P+ +DAE +  Q AI + T S +I
Sbjct: 325 MKQGSEPLLTSKDIKEIKEFFNRMDKIFQLCHQRGVPILVDAEQSYYQVAIHHLTMSYSI 384

Query: 137 IHNKDHNPIVYGTIQAYLKDAKDRLL--LVTKEATKMKVPLGIKLVRGAYMSSESKLASS 196
            +NK+  PI+Y T Q YL +  + L        + K    LG K+VRGAYM +ES+ +  
Sbjct: 385 KYNKE-KPIIYNTYQMYLVNGMNVLKQHFELSSSQKFNFKLGAKIVRGAYMVTESERSQR 444

Query: 197 LGFESPIHNTIQDTHFCYNTCASFLLDNIAK--GSSGAILATHNIESGNELCSML--YAL 256
           L  E+P+  TIQDTH  YNT   FLL+ I     S G ++A+HN +S N    ++  Y +
Sbjct: 445 LSTENPVLPTIQDTHKSYNTALDFLLNQIKSDPNSIGLMIASHNEDSINLGTKLIKQYKI 504

Query: 257 LISKPTQLFDNHFGF-------------QVSKYMPFGPVDMVMPYLLRRAEENRG 284
             + P   F   FG              ++ KY+PFGPV+ V+PYL+RR  EN+G
Sbjct: 505 DPTNPNIQFGQLFGMADFLSFNLVDQHQRIFKYVPFGPVEEVLPYLIRRMHENKG 550

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BLAST of Lsi03G002120 vs. ExPASy Swiss-Prot
Match: Q9WU79 (Proline dehydrogenase 1, mitochondrial OS=Mus musculus OX=10090 GN=Prodh PE=1 SV=2)

HSP 1 Score: 114.8 bits (286), Expect = 1.9e-24
Identity = 75/221 (33.94%), Postives = 116/221 (52.49%), Query Frame = 0


Query: 77  TNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNPI 136
           T EEE+ ++   +R+  + +   +A + L IDAE +  QPAI   T       N D  P 
Sbjct: 348 TEEEEQQMKRMLQRMDVLAKKAKEAGVRLMIDAEQSYFQPAISRLTLEMQRRFNVD-KPF 407

Query: 137 VYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNTI 196
           ++ T Q YLKDA D + L  + A +     G KLVRGAYM+ E   A+ +G+E PI+ T 
Sbjct: 408 IFNTFQCYLKDAYDNVTLDMELARREGWCFGAKLVRGAYMAQERVRAAEIGYEDPINPTY 467

Query: 197 QDTHFCYNTCASFLLDNIAKGSSGAIL-ATHNIESGN-ELCSM------------LYALL 256
           + T+  Y+ C +++L+ +   +   ++ A+HN ++ +  LC M             +  L
Sbjct: 468 EATNAMYHRCLNYVLEELKHSTKAEVMVASHNEDTVHFTLCRMKEIGLHPADGQVCFGQL 527

Query: 257 ISKPTQLFD--NHFGFQVSKYMPFGPVDMVMPYLLRRAEEN 282
           +    Q+       GF V KY+P+GPV  V+PYL RRA EN
Sbjct: 528 LGMCDQISFPLGQAGFPVYKYVPYGPVMEVLPYLSRRALEN 567

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BLAST of Lsi03G002120 vs. ExPASy Swiss-Prot
Match: O45228 (Proline dehydrogenase 1, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=prdh-1 PE=2 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 1.2e-23
Identity = 77/261 (29.50%), Postives = 128/261 (49.04%), Query Frame = 0


Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           L+NEEE+  +    R + + E  ++  + + +DAE T +QPAI   T      +NK    
Sbjct: 359 LSNEEEQEFRNMVRRTLDVAEYAIEKGVRIMVDAEQTYLQPAISKITIEMMKKYNKGRGN 418

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           I + T QAYLK     +    + A +     G KLVRGAYM  E   A ++G+E PI++ 
Sbjct: 419 I-FNTYQAYLKGTLQNMEADMQVARREGWHFGAKLVRGAYMEQERARAKAIGYEDPINDN 478

Query: 196 IQDTHFCYNTCASFLLDNI---AKGSSGAILATHNIESGNELCSMLYALLISKP------ 255
            + T   Y +C + + D +    K +   ++A+HN ++     +++    IS        
Sbjct: 479 FEATSKMYESCLTRIADEVHRRGKTNVSVMVASHNEDTVRFALNLMKEKCISPSERVMCM 538

Query: 256 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRGSGGLSREINRDGRI 315
            QL+             GF V KY+P+GPV+ V+PYL RRA EN   G + ++ N++  +
Sbjct: 539 AQLYGMCDQVSFSLGQAGFSVYKYLPYGPVEEVLPYLSRRALEN---GSVLKKANKERDL 598

Query: 316 SFSCLSRNRSLHRIQQPTAAS 319
            +  L R  S    +  +++S
Sbjct: 599 LWKELKRRISSGEFKARSSSS 615

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BLAST of Lsi03G002120 vs. ExPASy TrEMBL
Match: A0A0A0KXN4 (Proline dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G643300 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.5e-127
Identity = 239/296 (80.74%), Postives = 254/296 (85.81%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 185 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 244

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQMSHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 245 LTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 304

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+KVPLGIKLVRGAYMSSESKLASSLGFESPIH+T
Sbjct: 305 IVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESPIHDT 364

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGS+GAILATHN+ESG    S  Y + I K  Q  +    
Sbjct: 365 IQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLEFAQL 424

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L RE+ R
Sbjct: 425 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480

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BLAST of Lsi03G002120 vs. ExPASy TrEMBL
Match: A0A1S3CBE9 (Proline dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498577 PE=3 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 7.9e-127
Identity = 237/296 (80.07%), Postives = 254/296 (85.81%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 179 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 238

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQ+SHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 239 LTREEEKSLQISHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 298

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+K+PLGIKLVRGAYMSSESKLASSLGFESPIHNT
Sbjct: 299 IVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPIHNT 358

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGSSGAILATHN+ESG    +  Y + I K  Q  +    
Sbjct: 359 IQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEFAQL 418

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 419 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 474

[Close]

BLAST of Lsi03G002120 vs. ExPASy TrEMBL
Match: A0A5D3BNA9 (Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002050 PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 2.3e-126
Identity = 236/296 (79.73%), Postives = 253/296 (85.47%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 179 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 238

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQ+SHERLMKIC+SCVD N+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 239 LTREEEKSLQISHERLMKICQSCVDTNVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 298

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+K+PLGIKLVRGAYMSSESKLASSLGFESPIHNT
Sbjct: 299 IVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPIHNT 358

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGSSGAILATHN+ESG    +  Y + I K  Q  +    
Sbjct: 359 IQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEFAQL 418

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 419 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 474

[Close]

BLAST of Lsi03G002120 vs. ExPASy TrEMBL
Match: A0A6J1JZR8 (Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489341 PE=3 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 4.5e-122
Identity = 227/296 (76.69%), Postives = 249/296 (84.12%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVV KVSAICPLRLLERVSDLLRWQQK+P  NLPWKL+T PIFSESSPLYHTL+QP P
Sbjct: 178 ASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQPNP 237

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LTNEE+KSLQ+SH+RLMKIC+SCVDAN+PLAIDAEHTK+QPAIDYFTYSAAIIHNKDHNP
Sbjct: 238 LTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHNP 297

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGT+QAYLKDAKDRLLLVTKEA KM+VPLGIKLVRGAYMSSESKLASSLGFESPIHN+
Sbjct: 298 IVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNS 357

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           I DTH CYN+CASFLLDNIA GSSGAILATHN+ESG  +    Y + I K  +  +    
Sbjct: 358 IHDTHACYNSCASFLLDNIAAGSSGAILATHNVESGKLVAKKAYEIGIEKLKEKLEFAQL 417

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFG VDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 418 YGMSEALSYGLRNAGFQVSKYMPFGGVDMVMPYLLRRAEENRGLLSASNLDRQLMR 473

[Close]

BLAST of Lsi03G002120 vs. ExPASy TrEMBL
Match: A0A6J1FGN7 (Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 2.2e-121
Identity = 228/296 (77.03%), Postives = 249/296 (84.12%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVV KVSAICPLRLLERVSDLLRWQQK+P  NLPWKL+T PIFSESSPLYHTL+QP P
Sbjct: 184 ASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQPNP 243

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LTNEE+KSLQ+SH+RLMKIC+SCVDAN+PLAIDAEHTK+QPAIDYFTYSAAIIHNKDHNP
Sbjct: 244 LTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHNP 303

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGT+QAYLKDAKDRLLLVTKEA +M+VPLGIKLVRGAYMSSESKLASSLGFESPIHN+
Sbjct: 304 IVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNS 363

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKP------TQL 255
           I DTH CYN+CASFLLDNIA GSSGAILATHN+ESG       Y + I K        QL
Sbjct: 364 IHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEFAQL 423

Query: 256 FD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
           +           + GFQVSKYMPFG VDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 424 YGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLMR 479

[Close]

BLAST of Lsi03G002120 vs. NCBI nr
Match: XP_038891036.1 (proline dehydrogenase 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 468.0 bits (1203), Expect = 6.6e-128
Identity = 243/307 (79.15%), Postives = 259/307 (84.36%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQPKP
Sbjct: 183 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTLEQPKP 242

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT+EEEKSLQMSHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKDH P
Sbjct: 243 LTHEEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHIP 302

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLLV KEA+K+KVPLGIKLVRGAYMSSESK+ASSLGFESPIHNT
Sbjct: 303 IVYGTIQAYLKDAKDRLLLVNKEASKLKVPLGIKLVRGAYMSSESKIASSLGFESPIHNT 362

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLDNIAKGSSG ILATHN+ESG    +  Y + + K  Q  +    
Sbjct: 363 IQDTHVCYNTCASFLLDNIAKGSSGVILATHNVESGKLAATKAYEIGMGKLKQKLEFAQL 422

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINRD--G 303
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L R++ R   G
Sbjct: 423 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQLMRKELG 482

[Close]

BLAST of Lsi03G002120 vs. NCBI nr
Match: XP_004141603.2 (proline dehydrogenase 2, mitochondrial [Cucumis sativus] >KGN52551.1 hypothetical protein Csa_008665 [Cucumis sativus])

HSP 1 Score: 464.5 bits (1194), Expect = 7.3e-127
Identity = 239/296 (80.74%), Postives = 254/296 (85.81%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 185 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 244

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQMSHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 245 LTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 304

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+KVPLGIKLVRGAYMSSESKLASSLGFESPIH+T
Sbjct: 305 IVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESPIHDT 364

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGS+GAILATHN+ESG    S  Y + I K  Q  +    
Sbjct: 365 IQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLEFAQL 424

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L RE+ R
Sbjct: 425 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480

[Close]

BLAST of Lsi03G002120 vs. NCBI nr
Match: XP_008459446.1 (PREDICTED: proline dehydrogenase 2, mitochondrial [Cucumis melo])

HSP 1 Score: 463.4 bits (1191), Expect = 1.6e-126
Identity = 237/296 (80.07%), Postives = 254/296 (85.81%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 179 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 238

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQ+SHERLMKIC+SCVDAN+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 239 LTREEEKSLQISHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 298

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+K+PLGIKLVRGAYMSSESKLASSLGFESPIHNT
Sbjct: 299 IVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPIHNT 358

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGSSGAILATHN+ESG    +  Y + I K  Q  +    
Sbjct: 359 IQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEFAQL 418

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 419 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 474

[Close]

BLAST of Lsi03G002120 vs. NCBI nr
Match: KAA0039400.1 (proline dehydrogenase 2 [Cucumis melo var. makuwa] >TYK00588.1 proline dehydrogenase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 461.8 bits (1187), Expect = 4.7e-126
Identity = 236/296 (79.73%), Postives = 253/296 (85.47%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLEQP+P
Sbjct: 179 ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEP 238

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LT EEEKSLQ+SHERLMKIC+SCVD N+PLAIDAEHTKVQPAIDYFTYSAAIIHNKD NP
Sbjct: 239 LTREEEKSLQISHERLMKICQSCVDTNVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNP 298

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGTIQAYLKDAKDRLLL  KEA+K+K+PLGIKLVRGAYMSSESKLASSLGFESPIHNT
Sbjct: 299 IVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPIHNT 358

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           IQDTH CYNTCASFLLD+IAKGSSGAILATHN+ESG    +  Y + I K  Q  +    
Sbjct: 359 IQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEFAQL 418

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFGPVDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 419 YGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 474

[Close]

BLAST of Lsi03G002120 vs. NCBI nr
Match: XP_022993279.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 447.6 bits (1150), Expect = 9.3e-122
Identity = 227/296 (76.69%), Postives = 249/296 (84.12%), Query Frame = 0


Query: 16  ASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPKP 75
           ASFVV KVSAICPLRLLERVSDLLRWQQK+P  NLPWKL+T PIFSESSPLYHTL+QP P
Sbjct: 178 ASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQPNP 237

Query: 76  LTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHNP 135
           LTNEE+KSLQ+SH+RLMKIC+SCVDAN+PLAIDAEHTK+QPAIDYFTYSAAIIHNKDHNP
Sbjct: 238 LTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKDHNP 297

Query: 136 IVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIHNT 195
           IVYGT+QAYLKDAKDRLLLVTKEA KM+VPLGIKLVRGAYMSSESKLASSLGFESPIHN+
Sbjct: 298 IVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFESPIHNS 357

Query: 196 IQDTHFCYNTCASFLLDNIAKGSSGAILATHNIESGNELCSMLYALLISKPTQLFD---- 255
           I DTH CYN+CASFLLDNIA GSSGAILATHN+ESG  +    Y + I K  +  +    
Sbjct: 358 IHDTHACYNSCASFLLDNIAAGSSGAILATHNVESGKLVAKKAYEIGIEKLKEKLEFAQL 417

Query: 256 -----------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
                       + GFQVSKYMPFG VDMVMPYLLRRAEENRG   +  L R++ R
Sbjct: 418 YGMSEALSYGLRNAGFQVSKYMPFGGVDMVMPYLLRRAEENRGLLSASNLDRQLMR 473

[Close]

BLAST of Lsi03G002120 vs. TAIR 10
Match: AT5G38710.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 315.8 bits (808), Expect = 3.9e-86
Identity = 165/299 (55.18%), Postives = 208/299 (69.57%), Query Frame = 0


Query: 15  HASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPK 74
           H S VV K++AICP+ +L+RVSDLLRWQ KNP+F LPWKL + P+FS  SPLYHT  +P+
Sbjct: 168 HLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYHTTSEPE 227

Query: 75  PLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN 134
           PLT EEE+ L+ +HERL  +C  C ++N+PL IDAE T +QPAIDY  Y +AI+ N D +
Sbjct: 228 PLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIMFNSDKD 287

Query: 135 -PIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIH 194
            PIVY TIQAYLKDA +RL L  +E+ KM VP+G KLVRGAYMSSE+KLA SLG++SP+H
Sbjct: 288 RPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLGYKSPVH 347

Query: 195 NTIQDTHFCYNTCASFLLDNIAKGSS-GAILATHNIESGNELCSMLYALLISKP------ 254
           +TIQ+TH CYN C SFL++  + GS    ILATHN +SG         L I+K       
Sbjct: 348 DTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKENGKIEF 407

Query: 255 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
            QL+             GF VSKYMP+GPVD  +PYL+RRA ENRG   +G L R++ R
Sbjct: 408 AQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALDRQLMR 466

[Close]

BLAST of Lsi03G002120 vs. TAIR 10
Match: AT3G30775.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.4e-83
Identity = 162/299 (54.18%), Postives = 205/299 (68.56%), Query Frame = 0


Query: 15  HASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLETLPIFSESSPLYHTLEQPK 74
           H S VV K++AICP+ LL+RVSDLLRW+ K+P+F L WKL++ P+FSESSPLYHT  +P+
Sbjct: 189 HFSSVVVKITAICPISLLKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPE 248

Query: 75  PLTNEEEKSLQMSHERLMKICESCVDANIPLAIDAEHTKVQPAIDYFTYSAAIIHNKDHN 134
           PLT EEE+ L+ +H R+ +IC  C ++N+PL IDAE T +QPAIDY  YS+AI+ N D +
Sbjct: 249 PLTAEEERELEAAHGRIQEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKD 308

Query: 135 -PIVYGTIQAYLKDAKDRLLLVTKEATKMKVPLGIKLVRGAYMSSESKLASSLGFESPIH 194
            PIVY TIQAYL+DA +RL L  + A K  VP+G KLVRGAYMSSE+ LA SLG +SP+H
Sbjct: 309 RPIVYNTIQAYLRDAGERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVH 368

Query: 195 NTIQDTHFCYNTCASFLLDNIAKGSS-GAILATHNIESGNELCSMLYALLISKP------ 254
           +TIQDTH CYN C +FL++  + GS  G +LATHN +SG         L I K       
Sbjct: 369 DTIQDTHSCYNDCMTFLMEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEF 428

Query: 255 TQLFD---------NHFGFQVSKYMPFGPVDMVMPYLLRRAEENRG---SGGLSREINR 294
            QL+             GF VSKYMPFGPV   +PYLLRRA ENRG   +G   R++ R
Sbjct: 429 AQLYGMSDALSFGLKRAGFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMR 487

The following BLAST results are available for this feature:

BLAST of Lsi03G002120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NKX1	5.5e-85	55.18	Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 P...	[more]
P92983	2.0e-82	54.18	Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 P...	[more]
Q86H28	2.7e-31	33.56	Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=p...	[more]
Q9WU79	1.9e-24	33.94	Proline dehydrogenase 1, mitochondrial OS=Mus musculus OX=10090 GN=Prodh PE=1 SV...	[more]
O45228	1.2e-23	29.50	Proline dehydrogenase 1, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=prdh...	[more]

BLAST of Lsi03G002120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXN4	3.5e-127	80.74	Proline dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G643300 PE=3 SV=1	[more]
A0A1S3CBE9	7.9e-127	80.07	Proline dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498577 PE=3 SV=1	[more]
A0A5D3BNA9	2.3e-126	79.73	Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16...	[more]
A0A6J1JZR8	4.5e-122	76.69	Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489341 PE=3 SV=1	[more]
A0A6J1FGN7	2.2e-121	77.03	Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1	[more]

BLAST of Lsi03G002120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891036.1	6.6e-128	79.15	proline dehydrogenase 2, mitochondrial-like [Benincasa hispida]	[more]
XP_004141603.2	7.3e-127	80.74	proline dehydrogenase 2, mitochondrial [Cucumis sativus] >KGN52551.1 hypothetica...	[more]
XP_008459446.1	1.6e-126	80.07	PREDICTED: proline dehydrogenase 2, mitochondrial [Cucumis melo]	[more]
KAA0039400.1	4.7e-126	79.73	proline dehydrogenase 2 [Cucumis melo var. makuwa] >TYK00588.1 proline dehydroge...	[more]
XP_022993279.1	9.3e-122	76.69	proline dehydrogenase 2, mitochondrial-like [Cucurbita maxima]	[more]

BLAST of Lsi03G002120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G38710.1	3.9e-86	55.18	Methylenetetrahydrofolate reductase family protein	[more]
AT3G30775.1	1.4e-83	54.18	Methylenetetrahydrofolate reductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002872	Proline dehydrogenase domain	PFAM	PF01619	Pro_dh	coord: 70..284 e-value: 4.1E-51 score: 174.1
None	No IPR available	GENE3D	3.20.20.220		coord: 60..315 e-value: 6.8E-58 score: 198.5
None	No IPR available	PANTHER	PTHR13914:SF25	PROLINE DEHYDROGENASE	coord: 16..284
IPR015659	Proline oxidase family	PANTHER	PTHR13914	PROLINE OXIDASE	coord: 16..284
IPR029041	FAD-linked oxidoreductase-like	SUPERFAMILY	51730	FAD-linked oxidoreductase	coord: 14..289

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G002120.1	Lsi03G002120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010133	proline catabolic process to glutamate
biological_process	GO:0006562	proline catabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0004657	proline dehydrogenase activity