Lsi02G029170.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G029170.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	DNA-directed RNA polymerase I subunit rpa49
Location	chr02: 35409753 .. 35412633 (+)
Sequence length	1008
RNA-Seq Expression	Lsi02G029170.1
Synteny	Lsi02G029170.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGGAAGACACGAAATCTCTTGAATTAGAAGAAAACCATAACGCAGAACCAGACGCAAACTCACAGTCTCCAGCGAAGAAGAAGAAGAGTGAAAAAAGGAAGAAGCGAGTTCCATCAGTGAATGCTAAAATCCAAGTTCTCAGCAACCATCCCCAGAAAACTCCGCCGTTAGTGGGTTATTTCCCGTCTGGGTTTGATCCTCACAAGGATGCCGACGAGGAAGCAGCCGAGGAACAGAATTCCGTTAGGGCTAGGGTTTATAAGCACAAGAACAAACCAAATAGGATGCAGCTGGTTGTGAGTCCAACAGGGTCGAATGTGGATTTTGTTGGTACCAATTATTTGGGCGAGGCTACAGCTGGGAAACAGCATTGCACCTATGCTCTCGGTGTTCTTGATAAGGCCACCCAAATCTTAAAGATAGTGCCTATTGCATCAAATATGGTACTTTATTCTCGATAATGAATGTAGAGAATTAAGAATTGTGAAAAATTGAATGCTACTTGATGCGATTTTATGTATACTCACTGAAGTTACAAATTATTAGTATGTTATCTTTTGAATGAATTTGGCTCCTCGATCTGCTGTGCTTGACCTTTCTCTGAAAAGACCAGTAATACGGGTTGTGAACTGTTTTCATCGAGCTAGGAGATTACAATCACTGAAGTTTTGGTTGATTATTTTGGTGCTATTGTCAACTTTGTTGCATATAATTTTTTTCTTCTTCATTTTGCTCCAAACTCACTGCAGAAATTTCTAGAACTAGGCTTACCTTTGTGATGCATTTTATCTACTTTACAAAAGGTTAATGTAGTAACTAATAATCATAATTGTGTTGCTAAGTTTGATATTTATGCCTGCAGATATTTAGATTAGACCCAAAAGTCAGAGGTTCAGACGTTTCTGAAAATGAATCCCCAAGTGTGGCAAATGAAGAGCTTTCTGTGCAGGAGAAAGCTGATAGAATAAGAGAGCTCACCAATCTCTATGGAACAAAGAGGTCAAAAACGCAGGTAGAAATTGAATCCTGGAATTGCTCTCTTTAGTACTTCAGCAGCCAATTTTCTCTTGGGCATGTTTGGAATGAATTTTCAAGTGCTTAAAAAAGTGCCTTAGTGCATGTTTGGGAGTGATTTTGAAAGGGTTAAAATCACTTTCGTCATATTCAAAATCACTTCGAAATATGTCTTTAATCACTCAAAATTAATTTAATATTTAATTTTACACTTTTAAATGCACTTTTCATGTCATCAAAATTGCTTTTGAATGATTAGAAGGATGTTTAAGAGTGATTTTGGTGTAATACCAATTTCTAGAATTAAATCAAATCAATCTCTACCTCTAAATGAGGGGAGATGGACTTTATTTATAGGAAAATTTGAGTAAAAGTTTGTTAAAAGAAAACTAAGAAATAAAAAATAACAAACAAACTATTTAACAACTAACCATTTAACTAACTAACTATTAGTTAGCTTTAGAAAGGTCTTATCATGAAGCCAAATGGAAATCGTCAGGGGCAAAATAAGGCTTTAAGTCAGCAACATTGAGTATCGGATTAATCTGTAAATCACTCGGTGGAGTAGGGTGGTCAGAGGTACTCTTTGCTCTTCTACTTGCAGAAGCATCCCAAGGAGCTTTTCTTGAATTTTTCTTGGAGTGTAAAACATTCAACTCCTCTATTGTTTCAGCATAAGAAATTGCATCAGCTAACAAATGAAATTGTTGTAGTTTTACCTTTTTGATGTCCATTCGCAAGCCACCTGTAAATCTTATTTTGCTCCTGATGACTTCCAGGTGGTTTCCTACATGCCATCCTCGAGGTCGAGTTTGATTTCGGGGGGATGGAATATGGTGTAGGGTGAGGCTTTATTGTTGTAACTAGCTAAAGAGTTGGTTGGGATAGTTAATTAGTTAGATAGTTAGTTTATAATAAGTTTGGGATAGTTAGTTAGTTAGATAGTTAATAATAAGTTTGTTTGTCATTTTAATTTGTTAGCATTCTTTACAAACTTGTATACACATTTTCGCTATAAATAAAGGCCACTTCCCCTTATTTAGAGGTGGAGATTCATTTTCATTCCAATCAGATTTGGGTATTACACAAAATTTTGAAAATGACAAAAGTGATTTAAACTATTTGAGAATCCCAAACATGCCCTTATGTATTAAACGAATGTTAGACTTCAGCAGGTGAGCAGAATTTTGGGTGGCATGTTATCATGAAAAAGAATTACATCTTTTCTATTTACCTGTTTTGTTGCTTTTAATTTGTTTTGGATGGCATCTGTTCCTCTTTTCCACTATCATCATCTTTACCCTTTACTTGAAAATGAATCCCTTTGACATTTGAGATGTTTGACCATGACAGTAAGTAAAAGAAACTTTGTTTTGGATGATGAAGTCGCTTTCAAATGGCAGTTTCAGGAAGTAGCAACAAATCATTTGATAATTGTTAATTTTGTTGCAGGCAATGAAGCTGCATGCATTGAAGCAAGAGGATGATCCTGATACCAAGAATGATCTAGATGTGAGAATGAAAAATGTAGTGGTAAACAAAGAGGCCTTGGGAGGTACGCATGCAGAAATTGCTCGTAATATACCAACATATAATGCTTCTGCAACTACTCCACAAGAAGCTTACCCACTCGACAAGATTATCCTCAAGGGAGAATGGAGTTTTCTGGAAGATCTCTACTTTCGTTTGCAAGAAACAGCTGAAATAAGTGCTAATTATCCATCCTTTGTCCGCAACAGAATTTATAAATTGCAAAACATTCAGGTACGGTGTCAAGAAGGTTCTGCTTGTAATTACCTTTATGAATATTCTGAGATAAAACATTCTTCCTTTCTGATACATATTCATGAATCATGTGGATGA

mRNA sequence

ATGACGGAAGACACGAAATCTCTTGAATTAGAAGAAAACCATAACGCAGAACCAGACGCAAACTCACAGTCTCCAGCGAAGAAGAAGAAGAGTGAAAAAAGGAAGAAGCGAGTTCCATCAGTGAATGCTAAAATCCAAGTTCTCAGCAACCATCCCCAGAAAACTCCGCCGTTAGTGGGTTATTTCCCGTCTGGGTTTGATCCTCACAAGGATGCCGACGAGGAAGCAGCCGAGGAACAGAATTCCGTTAGGGCTAGGGTTTATAAGCACAAGAACAAACCAAATAGGATGCAGCTGGTTGTGAGTCCAACAGGGTCGAATGTGGATTTTGTTGGTACCAATTATTTGGGCGAGGCTACAGCTGGGAAACAGCATTGCACCTATGCTCTCGGTGTTCTTGATAAGGCCACCCAAATCTTAAAGATAGTGCCTATTGCATCAAATATGATATTTAGATTAGACCCAAAAGTCAGAGGTTCAGACGTTTCTGAAAATGAATCCCCAAGTGTGGCAAATGAAGAGCTTTCTGTGCAGGAGAAAGCTGATAGAATAAGAGAGCTCACCAATCTCTATGGAACAAAGAGGTCAAAAACGCAGGCAATGAAGCTGCATGCATTGAAGCAAGAGGATGATCCTGATACCAAGAATGATCTAGATGTGAGAATGAAAAATGTAGTGGTAAACAAAGAGGCCTTGGGAGGTACGCATGCAGAAATTGCTCGTAATATACCAACATATAATGCTTCTGCAACTACTCCACAAGAAGCTTACCCACTCGACAAGATTATCCTCAAGGGAGAATGGAGTTTTCTGGAAGATCTCTACTTTCGTTTGCAAGAAACAGCTGAAATAAGTGCTAATTATCCATCCTTTGTCCGCAACAGAATTTATAAATTGCAAAACATTCAGGTACGGTGTCAAGAAGGTTCTGCTTGTAATTACCTTTATGAATATTCTGAGATAAAACATTCTTCCTTTCTGATACATATTCATGAATCATGTGGATGA

Coding sequence (CDS)

ATGACGGAAGACACGAAATCTCTTGAATTAGAAGAAAACCATAACGCAGAACCAGACGCAAACTCACAGTCTCCAGCGAAGAAGAAGAAGAGTGAAAAAAGGAAGAAGCGAGTTCCATCAGTGAATGCTAAAATCCAAGTTCTCAGCAACCATCCCCAGAAAACTCCGCCGTTAGTGGGTTATTTCCCGTCTGGGTTTGATCCTCACAAGGATGCCGACGAGGAAGCAGCCGAGGAACAGAATTCCGTTAGGGCTAGGGTTTATAAGCACAAGAACAAACCAAATAGGATGCAGCTGGTTGTGAGTCCAACAGGGTCGAATGTGGATTTTGTTGGTACCAATTATTTGGGCGAGGCTACAGCTGGGAAACAGCATTGCACCTATGCTCTCGGTGTTCTTGATAAGGCCACCCAAATCTTAAAGATAGTGCCTATTGCATCAAATATGATATTTAGATTAGACCCAAAAGTCAGAGGTTCAGACGTTTCTGAAAATGAATCCCCAAGTGTGGCAAATGAAGAGCTTTCTGTGCAGGAGAAAGCTGATAGAATAAGAGAGCTCACCAATCTCTATGGAACAAAGAGGTCAAAAACGCAGGCAATGAAGCTGCATGCATTGAAGCAAGAGGATGATCCTGATACCAAGAATGATCTAGATGTGAGAATGAAAAATGTAGTGGTAAACAAAGAGGCCTTGGGAGGTACGCATGCAGAAATTGCTCGTAATATACCAACATATAATGCTTCTGCAACTACTCCACAAGAAGCTTACCCACTCGACAAGATTATCCTCAAGGGAGAATGGAGTTTTCTGGAAGATCTCTACTTTCGTTTGCAAGAAACAGCTGAAATAAGTGCTAATTATCCATCCTTTGTCCGCAACAGAATTTATAAATTGCAAAACATTCAGGTACGGTGTCAAGAAGGTTCTGCTTGTAATTACCTTTATGAATATTCTGAGATAAAACATTCTTCCTTTCTGATACATATTCATGAATCATGTGGATGA

Protein sequence

MTEDTKSLELEENHNAEPDANSQSPAKKKKSEKRKKRVPSVNAKIQVLSNHPQKTPPLVGYFPSGFDPHKDADEEAAEEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEATAGKQHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQEKADRIRELTNLYGTKRSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHAEIARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISANYPSFVRNRIYKLQNIQVRCQEGSACNYLYEYSEIKHSSFLIHIHESCG

Homology

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BLAST of Lsi02G029170.1 vs. ExPASy Swiss-Prot
Match: Q54FE8 (DNA-directed RNA polymerase I subunit rpa49 OS=Dictyostelium discoideum OX=44689 GN=polr1e PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 3.8e-04
Identity = 42/184 (22.83%), Postives = 94/184 (51.09%), Query Frame = 0


Query: 124 QHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQEKADR 183
           ++  YA+G+ DK T+ LKIVP     I  ++  V G  ++ + S  ++ E     E+ D+
Sbjct: 70  KYAKYAIGIFDKNTKQLKIVP---TKIQTMNQSVIGYTLTLDSSSVISRE-----ERYDK 129

Query: 184 IRELTNLYGTK--RSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHAEIAR 243
           I++L + +G+K  + K Q M++  ++  D   T   +D  +KN + + E+      E   
Sbjct: 130 IKKLADSFGSKITKKKMQKMEMENVENLDYEKTTKSIDA-VKNELADIES-----NEKPF 189

Query: 244 NIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFR--LQETAEISANYPSFVRNRIYKL 303
           ++P ++A     ++ Y ++ I+    +  L    F   + +T+E  +  P + ++++ + 
Sbjct: 190 DLPDFDAETNDVRKIYNIEAILPPRIFFGLNHQPFLDIISKTSEAPSYLPEYFKDQLKRF 239

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BLAST of Lsi02G029170.1 vs. ExPASy TrEMBL
Match: A0A5D3BXN3 (DNA-directed RNA polymerase I subunit rpa49 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001060 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.4e-142
Identity = 270/313 (86.26%), Postives = 287/313 (91.69%), Query Frame = 0


Query: 1   MTEDTKSLELEENHNAEPDANSQSPA-KKKKSEKRKKRVPSVNAKIQVLSNHPQKTPPLV 60
           M EDTKSLELEENHNAEPDANSQSPA KKKKSEK+KKRV S+NAKIQVLSN PQK PP+V
Sbjct: 1   MAEDTKSLELEENHNAEPDANSQSPAKKKKKSEKKKKRVLSMNAKIQVLSNQPQKIPPVV 60

Query: 61  GYFPSGFDPHKDADEEAAEEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
           GYFPSGFDPHKDAD+   E+ +SVRARVYKH+NKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  GYFPSGFDPHKDADK---EQSHSVRARVYKHRNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQE 180
           TAGKQHCTYALGVLDKATQ LKIVPIASNMI RLDPKVRGSDVSENESPSVANEELS+QE
Sbjct: 121 TAGKQHCTYALGVLDKATQSLKIVPIASNMILRLDPKVRGSDVSENESPSVANEELSLQE 180

Query: 181 KADRIRELTNLYGTKRSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHAEI 240
           KA+R+RELT LYGTK+SK QA KLHALKQEDDP+TKNDLD RMKNVVVNKEAL GT AEI
Sbjct: 181 KANRMRELTALYGTKKSKKQAQKLHALKQEDDPETKNDLDGRMKNVVVNKEALEGTQAEI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISANYPSFVRNRIYKL 300
           ARNIP YNASATTPQE YPLDKIILKGEWSFLEDLYFRL+ETAEI+A YPSFVRNRIYKL
Sbjct: 241 ARNIPAYNASATTPQEVYPLDKIILKGEWSFLEDLYFRLRETAEINATYPSFVRNRIYKL 300

Query: 301 QNIQVRCQEGSAC 313
           Q+IQ   ++   C
Sbjct: 301 QDIQDEEEKKKLC 310

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BLAST of Lsi02G029170.1 vs. ExPASy TrEMBL
Match: A0A1S3CH93 (DNA-directed RNA polymerase I subunit rpa49 OS=Cucumis melo OX=3656 GN=LOC103500711 PE=3 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 4.0e-142
Identity = 269/313 (85.94%), Postives = 286/313 (91.37%), Query Frame = 0


Query: 1   MTEDTKSLELEENHNAEPDANSQSPA-KKKKSEKRKKRVPSVNAKIQVLSNHPQKTPPLV 60
           M EDTKSLE EENHNAEPDANSQSPA KKKKSEK+KKRV S+NAKIQVLSN PQK PP+V
Sbjct: 1   MAEDTKSLEFEENHNAEPDANSQSPAKKKKKSEKKKKRVLSMNAKIQVLSNQPQKIPPVV 60

Query: 61  GYFPSGFDPHKDADEEAAEEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
           GYFPSGFDPHKDAD+   E+ +SVRARVYKH+NKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  GYFPSGFDPHKDADK---EQSHSVRARVYKHRNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQE 180
           TAGKQHCTYALGVLDKATQ LKIVPIASNMI RLDPKVRGSDVSENESPSVANEELS+QE
Sbjct: 121 TAGKQHCTYALGVLDKATQSLKIVPIASNMILRLDPKVRGSDVSENESPSVANEELSLQE 180

Query: 181 KADRIRELTNLYGTKRSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHAEI 240
           KA+R+RELT LYGTK+SK QA KLHALKQEDDP+TKNDLD RMKNVVVNKEAL GT AEI
Sbjct: 181 KANRMRELTALYGTKKSKKQAQKLHALKQEDDPETKNDLDGRMKNVVVNKEALEGTQAEI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISANYPSFVRNRIYKL 300
           ARNIP YNASATTPQE YPLDKIILKGEWSFLEDLYFRL+ETAEI+A YPSFVRNRIYKL
Sbjct: 241 ARNIPAYNASATTPQEVYPLDKIILKGEWSFLEDLYFRLRETAEINATYPSFVRNRIYKL 300

Query: 301 QNIQVRCQEGSAC 313
           Q+IQ   ++   C
Sbjct: 301 QDIQDEEEKKKLC 310

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BLAST of Lsi02G029170.1 vs. ExPASy TrEMBL
Match: A0A6J1JX25 (DNA-directed RNA polymerase I subunit rpa49-like OS=Cucurbita maxima OX=3661 GN=LOC111488633 PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.9e-139
Identity = 264/324 (81.48%), Postives = 288/324 (88.89%), Query Frame = 0


Query: 1   MTEDTKSLELEENHNAEPDANSQSPA-KKKKSEKRKKRVPSVNAKIQVLSNHPQKTPPLV 60
           M EDTKS ELE+NH +EPDANSQSPA KKKKSEK+KKRVPSVNAKIQVLSNHPQKTPPLV
Sbjct: 1   MAEDTKSFELEDNHYSEPDANSQSPAKKKKKSEKKKKRVPSVNAKIQVLSNHPQKTPPLV 60

Query: 61  GYFPSGFDPHKDADEEAAEEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
            YFPSGFDPHKDA+EEA EEQ+SVRARVY+HKNKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  CYFPSGFDPHKDANEEAVEEQDSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQE 180
           TAGKQHC+Y LGVLDKATQ LKIVPIASNMIFRLDP++RGSDVSE ES SV NEELSVQE
Sbjct: 121 TAGKQHCSYGLGVLDKATQSLKIVPIASNMIFRLDPRIRGSDVSEMESQSVENEELSVQE 180

Query: 181 KADRIRELTNLYGTKRSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHAEI 240
           KA+++RELTNLYGTK+S  QA KLH+LKQEDDPDTK DLD +MKNVVVNKEAL  T A+I
Sbjct: 181 KAEKMRELTNLYGTKKSINQAKKLHSLKQEDDPDTKKDLDGKMKNVVVNKEALESTGAQI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISANYPSFVRNRIYKL 300
           ARNIPTYN SATTP+EAYPLDKIILKGEWSFLED+Y  LQE AEISA YPSFVRNRIYKL
Sbjct: 241 ARNIPTYNVSATTPREAYPLDKIILKGEWSFLEDIYSLLQEEAEISAKYPSFVRNRIYKL 300

Query: 301 QNIQVRCQEGSACNYLYEYSEIKH 324
           Q++Q   ++   C+    +S I H
Sbjct: 301 QDVQDEEEKKQLCSI---FSYITH 321

[Close]

BLAST of Lsi02G029170.1 vs. ExPASy TrEMBL
Match: A0A6J1GQS4 (DNA-directed RNA polymerase I subunit rpa49-like OS=Cucurbita moschata OX=3662 GN=LOC111456250 PE=3 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 7.8e-138
Identity = 261/324 (80.56%), Postives = 286/324 (88.27%), Query Frame = 0


Query: 1   MTEDTKSLELEENHNAEPDANSQSPA-KKKKSEKRKKRVPSVNAKIQVLSNHPQKTPPLV 60
           M EDTKS ELE+NH +EPD+N QSPA KKKKSEK+KKRVPSVNAKIQVLSNHPQKTPPLV
Sbjct: 1   MAEDTKSFELEDNHYSEPDSNLQSPAKKKKKSEKKKKRVPSVNAKIQVLSNHPQKTPPLV 60

Query: 61  GYFPSGFDPHKDADEEAAEEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
            YFPSGFDPHKD +EEA EEQ+SVRARVY+HKNKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  CYFPSGFDPHKDVNEEAVEEQDSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQE 180
           TAGKQHC+Y LGVLDKATQ LKIVPIASNMIFRLDP++RGSDVSE ESPSV NEELSVQE
Sbjct: 121 TAGKQHCSYGLGVLDKATQSLKIVPIASNMIFRLDPRIRGSDVSEMESPSVENEELSVQE 180

Query: 181 KADRIRELTNLYGTKRSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHAEI 240
           KA+++RELTNLYGTK+S  QA KL +LKQEDDPDTK DLD +MKNVVVNKEAL  T A+I
Sbjct: 181 KAEKMRELTNLYGTKKSINQAKKLRSLKQEDDPDTKKDLDGKMKNVVVNKEALESTGAQI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISANYPSFVRNRIYKL 300
           ARNIPTYN SATTP+EAYPLDKIILKGEWS+LEDLY  LQE AEISA YPSFVRNRIYKL
Sbjct: 241 ARNIPTYNVSATTPREAYPLDKIILKGEWSYLEDLYSLLQEEAEISAKYPSFVRNRIYKL 300

Query: 301 QNIQVRCQEGSACNYLYEYSEIKH 324
           Q++Q   ++   C+    +S I H
Sbjct: 301 QDVQDEEEKKQLCSI---FSYITH 321

[Close]

BLAST of Lsi02G029170.1 vs. ExPASy TrEMBL
Match: A0A0A0KCN4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450680 PE=3 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 1.5e-136
Identity = 262/313 (83.71%), Postives = 283/313 (90.42%), Query Frame = 0


Query: 1   MTEDTKSLELEENHNAEPDANSQSPA-KKKKSEKRKKRVPSVNAKIQVLSNHPQKTPPLV 60
           M +DTKS+ELEENH     A+SQSPA KKKKSEK+KKRV S++AKIQVLSN PQKTPP+V
Sbjct: 4   MADDTKSVELEENHY----ASSQSPAKKKKKSEKKKKRVLSMDAKIQVLSNQPQKTPPVV 63

Query: 61  GYFPSGFDPHKDADEEAAEEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
           GYFPSGFDPHKDAD+   E+  SVRARVYKHKN+PNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 64  GYFPSGFDPHKDADK---EQSRSVRARVYKHKNRPNRMQLVVSPTGSNVDFVGTNYLGEA 123

Query: 121 TAGKQHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQE 180
           TAGKQHCTYALGVLDKATQ LKIVPIASNMIFRLDPKVRGSDV+ENESPSVANEELS Q+
Sbjct: 124 TAGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVTENESPSVANEELSGQD 183

Query: 181 KADRIRELTNLYGTKRSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHAEI 240
           KA+R+RELT LYGTK+SK QA KLHALKQEDDP+TK+DLD RMKNVVVNKEAL GT AE+
Sbjct: 184 KANRMRELTALYGTKKSKKQAQKLHALKQEDDPETKSDLDGRMKNVVVNKEALEGTQAEV 243

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISANYPSFVRNRIYKL 300
           ARNIP YNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISA YPSFVRNRIYKL
Sbjct: 244 ARNIPAYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISATYPSFVRNRIYKL 303

Query: 301 QNIQVRCQEGSAC 313
           Q+IQ   ++   C
Sbjct: 304 QDIQDEEEKKKLC 309

[Close]

BLAST of Lsi02G029170.1 vs. NCBI nr
Match: XP_038896156.1 (DNA-directed RNA polymerase I subunit rpa49 [Benincasa hispida])

HSP 1 Score: 528.5 bits (1360), Expect = 4.3e-146
Identity = 278/313 (88.82%), Postives = 288/313 (92.01%), Query Frame = 0


Query: 1   MTEDTKSLELEENHNAEPDANSQSPA-KKKKSEKRKKRVPSVNAKIQVLSNHPQKTPPLV 60
           M EDTKSLELEENH AE D NS+SPA KKKKSEK+KKRVPSVNAKIQVL NHPQKTPP+V
Sbjct: 1   MAEDTKSLELEENHYAERDTNSESPAKKKKKSEKKKKRVPSVNAKIQVLGNHPQKTPPIV 60

Query: 61  GYFPSGFDPHKDADEEAAEEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
           GYFPSGFDPHKDAD    EEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  GYFPSGFDPHKDAD----EEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQE 180
           TAGKQHCTYALGVLDKATQ LKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQE
Sbjct: 121 TAGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQE 180

Query: 181 KADRIRELTNLYGTKRSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHAEI 240
           KADR+RELT LYGTK+SK QA KLHALKQEDDPDTK DLDVRMKNVVVN EAL GT AEI
Sbjct: 181 KADRMRELTVLYGTKKSKKQAQKLHALKQEDDPDTKKDLDVRMKNVVVNIEALEGTQAEI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISANYPSFVRNRIYKL 300
           ARNIPTYNASATTPQEAYPLDKIILKGEW+FLEDLYFRLQETAEISANYP FVR+RIYKL
Sbjct: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWNFLEDLYFRLQETAEISANYPYFVRHRIYKL 300

Query: 301 QNIQVRCQEGSAC 313
           Q+IQ   ++   C
Sbjct: 301 QDIQDEEEKKKLC 309

[Close]

BLAST of Lsi02G029170.1 vs. NCBI nr
Match: KAA0059416.1 (DNA-directed RNA polymerase I subunit rpa49 [Cucumis melo var. makuwa] >TYK03910.1 DNA-directed RNA polymerase I subunit rpa49 [Cucumis melo var. makuwa])

HSP 1 Score: 515.8 bits (1327), Expect = 2.9e-142
Identity = 270/313 (86.26%), Postives = 287/313 (91.69%), Query Frame = 0


Query: 1   MTEDTKSLELEENHNAEPDANSQSPA-KKKKSEKRKKRVPSVNAKIQVLSNHPQKTPPLV 60
           M EDTKSLELEENHNAEPDANSQSPA KKKKSEK+KKRV S+NAKIQVLSN PQK PP+V
Sbjct: 1   MAEDTKSLELEENHNAEPDANSQSPAKKKKKSEKKKKRVLSMNAKIQVLSNQPQKIPPVV 60

Query: 61  GYFPSGFDPHKDADEEAAEEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
           GYFPSGFDPHKDAD+   E+ +SVRARVYKH+NKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  GYFPSGFDPHKDADK---EQSHSVRARVYKHRNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQE 180
           TAGKQHCTYALGVLDKATQ LKIVPIASNMI RLDPKVRGSDVSENESPSVANEELS+QE
Sbjct: 121 TAGKQHCTYALGVLDKATQSLKIVPIASNMILRLDPKVRGSDVSENESPSVANEELSLQE 180

Query: 181 KADRIRELTNLYGTKRSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHAEI 240
           KA+R+RELT LYGTK+SK QA KLHALKQEDDP+TKNDLD RMKNVVVNKEAL GT AEI
Sbjct: 181 KANRMRELTALYGTKKSKKQAQKLHALKQEDDPETKNDLDGRMKNVVVNKEALEGTQAEI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISANYPSFVRNRIYKL 300
           ARNIP YNASATTPQE YPLDKIILKGEWSFLEDLYFRL+ETAEI+A YPSFVRNRIYKL
Sbjct: 241 ARNIPAYNASATTPQEVYPLDKIILKGEWSFLEDLYFRLRETAEINATYPSFVRNRIYKL 300

Query: 301 QNIQVRCQEGSAC 313
           Q+IQ   ++   C
Sbjct: 301 QDIQDEEEKKKLC 310

[Close]

BLAST of Lsi02G029170.1 vs. NCBI nr
Match: XP_008462335.1 (PREDICTED: DNA-directed RNA polymerase I subunit rpa49 [Cucumis melo])

HSP 1 Score: 514.2 bits (1323), Expect = 8.3e-142
Identity = 269/313 (85.94%), Postives = 286/313 (91.37%), Query Frame = 0


Query: 1   MTEDTKSLELEENHNAEPDANSQSPA-KKKKSEKRKKRVPSVNAKIQVLSNHPQKTPPLV 60
           M EDTKSLE EENHNAEPDANSQSPA KKKKSEK+KKRV S+NAKIQVLSN PQK PP+V
Sbjct: 1   MAEDTKSLEFEENHNAEPDANSQSPAKKKKKSEKKKKRVLSMNAKIQVLSNQPQKIPPVV 60

Query: 61  GYFPSGFDPHKDADEEAAEEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
           GYFPSGFDPHKDAD+   E+ +SVRARVYKH+NKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  GYFPSGFDPHKDADK---EQSHSVRARVYKHRNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQE 180
           TAGKQHCTYALGVLDKATQ LKIVPIASNMI RLDPKVRGSDVSENESPSVANEELS+QE
Sbjct: 121 TAGKQHCTYALGVLDKATQSLKIVPIASNMILRLDPKVRGSDVSENESPSVANEELSLQE 180

Query: 181 KADRIRELTNLYGTKRSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHAEI 240
           KA+R+RELT LYGTK+SK QA KLHALKQEDDP+TKNDLD RMKNVVVNKEAL GT AEI
Sbjct: 181 KANRMRELTALYGTKKSKKQAQKLHALKQEDDPETKNDLDGRMKNVVVNKEALEGTQAEI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISANYPSFVRNRIYKL 300
           ARNIP YNASATTPQE YPLDKIILKGEWSFLEDLYFRL+ETAEI+A YPSFVRNRIYKL
Sbjct: 241 ARNIPAYNASATTPQEVYPLDKIILKGEWSFLEDLYFRLRETAEINATYPSFVRNRIYKL 300

Query: 301 QNIQVRCQEGSAC 313
           Q+IQ   ++   C
Sbjct: 301 QDIQDEEEKKKLC 310

[Close]

BLAST of Lsi02G029170.1 vs. NCBI nr
Match: XP_022992259.1 (DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita maxima])

HSP 1 Score: 505.4 bits (1300), Expect = 3.9e-139
Identity = 264/324 (81.48%), Postives = 288/324 (88.89%), Query Frame = 0


Query: 1   MTEDTKSLELEENHNAEPDANSQSPA-KKKKSEKRKKRVPSVNAKIQVLSNHPQKTPPLV 60
           M EDTKS ELE+NH +EPDANSQSPA KKKKSEK+KKRVPSVNAKIQVLSNHPQKTPPLV
Sbjct: 1   MAEDTKSFELEDNHYSEPDANSQSPAKKKKKSEKKKKRVPSVNAKIQVLSNHPQKTPPLV 60

Query: 61  GYFPSGFDPHKDADEEAAEEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
            YFPSGFDPHKDA+EEA EEQ+SVRARVY+HKNKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  CYFPSGFDPHKDANEEAVEEQDSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQE 180
           TAGKQHC+Y LGVLDKATQ LKIVPIASNMIFRLDP++RGSDVSE ES SV NEELSVQE
Sbjct: 121 TAGKQHCSYGLGVLDKATQSLKIVPIASNMIFRLDPRIRGSDVSEMESQSVENEELSVQE 180

Query: 181 KADRIRELTNLYGTKRSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHAEI 240
           KA+++RELTNLYGTK+S  QA KLH+LKQEDDPDTK DLD +MKNVVVNKEAL  T A+I
Sbjct: 181 KAEKMRELTNLYGTKKSINQAKKLHSLKQEDDPDTKKDLDGKMKNVVVNKEALESTGAQI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISANYPSFVRNRIYKL 300
           ARNIPTYN SATTP+EAYPLDKIILKGEWSFLED+Y  LQE AEISA YPSFVRNRIYKL
Sbjct: 241 ARNIPTYNVSATTPREAYPLDKIILKGEWSFLEDIYSLLQEEAEISAKYPSFVRNRIYKL 300

Query: 301 QNIQVRCQEGSACNYLYEYSEIKH 324
           Q++Q   ++   C+    +S I H
Sbjct: 301 QDVQDEEEKKQLCSI---FSYITH 321

[Close]

BLAST of Lsi02G029170.1 vs. NCBI nr
Match: XP_023547949.1 (DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 503.1 bits (1294), Expect = 1.9e-138
Identity = 262/324 (80.86%), Postives = 287/324 (88.58%), Query Frame = 0


Query: 1   MTEDTKSLELEENHNAEPDANSQSPA-KKKKSEKRKKRVPSVNAKIQVLSNHPQKTPPLV 60
           M EDTKS ELE+NH +EPD+N QSPA KKKKSEK+KKRVPSVNAKIQVLSNHPQKTPPLV
Sbjct: 1   MAEDTKSFELEDNHYSEPDSNLQSPAKKKKKSEKKKKRVPSVNAKIQVLSNHPQKTPPLV 60

Query: 61  GYFPSGFDPHKDADEEAAEEQNSVRARVYKHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
            YFPSGFDPHKD +EEA EEQ+SVRARVY+HKNKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  CYFPSGFDPHKDVNEEAVEEQDSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENESPSVANEELSVQE 180
           TAGKQHC+Y LGVLDKATQ LKIVPIASNMIFRLDP++RGSDVSE ESPSV NEELSVQE
Sbjct: 121 TAGKQHCSYGLGVLDKATQSLKIVPIASNMIFRLDPRIRGSDVSEMESPSVENEELSVQE 180

Query: 181 KADRIRELTNLYGTKRSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHAEI 240
           KA+++RELTNLYGTK+S  QA KLH+LKQEDDPDTK DLD +MKNVVVNKEAL  T A+I
Sbjct: 181 KAEKMRELTNLYGTKKSINQAKKLHSLKQEDDPDTKKDLDGKMKNVVVNKEALESTGAQI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISANYPSFVRNRIYKL 300
           ARNIPTYN SATTP+EAYPLDKIILKGEWS+LEDLY  LQE AEISA YPSFVRNRIYKL
Sbjct: 241 ARNIPTYNVSATTPREAYPLDKIILKGEWSYLEDLYSLLQEEAEISAKYPSFVRNRIYKL 300

Query: 301 QNIQVRCQEGSACNYLYEYSEIKH 324
           Q++Q   ++   C+    +S I H
Sbjct: 301 QDVQDEEEKKQLCSI---FSYITH 321

[Close]

BLAST of Lsi02G029170.1 vs. TAIR 10
Match: AT3G13940.1 (DNA binding;DNA-directed RNA polymerases )

HSP 1 Score: 200.7 bits (509), Expect = 1.9e-51
Identity = 123/316 (38.92%), Postives = 183/316 (57.91%), Query Frame = 0


Query: 9   ELEENHNAEPDANSQSPAKKK---------KSEKRKKRVPSVNAKIQVLSNHPQKTPPLV 68
           E  +  N E D N+    +KK         ++E   +    V  K+  ++  P +  P+V
Sbjct: 5   EKNDYRNEEEDFNTPQQLQKKQRKGKKIATETEDENEEDKRVTLKVTQVAERPDRISPIV 64

Query: 69  GYFPSGFDPHKDADEEAAEEQNSVRARVYKHK-NKPNRMQLVVSPTGSNVDFVGTNYLGE 128
            YF +G+DP K   +       + +  VYKHK +   R+Q+VVSP G+ V+FVGTNY GE
Sbjct: 65  AYFSTGYDPCKVDPKTGKRVHETPKVTVYKHKDDSKKRIQVVVSPPGARVEFVGTNYTGE 124

Query: 129 ATAGKQHCTYALGVLDKATQILKIVPIASNMIFRLDPKVRGSDVSENE-SPSVANEELSV 188
             A  Q  TY +GV ++  + L+I+P+A N I RL+P+V+  + +E E S S   +EL  
Sbjct: 125 -QAAMQTNTYRVGVFNREAKTLRILPVAHNKIIRLEPRVKAQETNEEEASGSAVVKELEE 184

Query: 189 QEKADRIRELTNLYGTKRSKTQAMKLHALKQEDDPDTKNDLDVRMKNVVVNKEALGGTHA 248
            +  +R R     Y TK++ T+  K  AL   DD  T+  LD ++  + VN  AL GT +
Sbjct: 185 LKTGERDR-----YNTKKAVTRDKKKRALYMGDDAATQKVLDGKLSELGVNTAALEGTSS 244

Query: 249 EIARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFRLQETAEISAN-YPSFVRNRI 308
            +ARNIP YN +ATT  EAYPL+KII KG+WSFLED+Y+ LQ+  E +   YP FVRNR+
Sbjct: 245 TVARNIPPYNTAATTANEAYPLEKIIEKGDWSFLEDIYWLLQQETEAATEAYPVFVRNRL 304

Query: 309 YKLQNIQVRCQEGSAC 313
           Y+L++I+   ++ + C
Sbjct: 305 YRLRDIKDDMKKQTVC 314

The following BLAST results are available for this feature:

BLAST of Lsi02G029170.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54FE8	3.8e-04	22.83	DNA-directed RNA polymerase I subunit rpa49 OS=Dictyostelium discoideum OX=44689...	[more]

BLAST of Lsi02G029170.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BXN3	1.4e-142	86.26	DNA-directed RNA polymerase I subunit rpa49 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3CH93	4.0e-142	85.94	DNA-directed RNA polymerase I subunit rpa49 OS=Cucumis melo OX=3656 GN=LOC103500...	[more]
A0A6J1JX25	1.9e-139	81.48	DNA-directed RNA polymerase I subunit rpa49-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1GQS4	7.8e-138	80.56	DNA-directed RNA polymerase I subunit rpa49-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A0A0KCN4	1.5e-136	83.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450680 PE=3 SV=1	[more]

BLAST of Lsi02G029170.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896156.1	4.3e-146	88.82	DNA-directed RNA polymerase I subunit rpa49 [Benincasa hispida]	[more]
KAA0059416.1	2.9e-142	86.26	DNA-directed RNA polymerase I subunit rpa49 [Cucumis melo var. makuwa] >TYK03910...	[more]
XP_008462335.1	8.3e-142	85.94	PREDICTED: DNA-directed RNA polymerase I subunit rpa49 [Cucumis melo]	[more]
XP_022992259.1	3.9e-139	81.48	DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita maxima]	[more]
XP_023547949.1	1.9e-138	80.86	DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi02G029170.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13940.1	1.9e-51	38.92	DNA binding;DNA-directed RNA polymerases	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009668	RNA polymerase I associated factor, A49-like	PFAM	PF06870	RNA_pol_I_A49	coord: 82..303 e-value: 3.7E-26 score: 91.9
IPR009668	RNA polymerase I associated factor, A49-like	PANTHER	PTHR14440	DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49	coord: 27..312
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..16
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..57

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi02G029170	Lsi02G029170	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi02G029170.1.exon.1	Lsi02G029170.1.exon.1	exon
Lsi02G029170.1.exon.2	Lsi02G029170.1.exon.2	exon
Lsi02G029170.1.exon.3	Lsi02G029170.1.exon.3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi02G029170.1.CDS.1	Lsi02G029170.1.CDS.1	CDS
Lsi02G029170.1.CDS.2	Lsi02G029170.1.CDS.2	CDS
Lsi02G029170.1.CDS.3	Lsi02G029170.1.CDS.3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi02G029170.1	Lsi02G029170.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
biological_process	GO:0042790	nucleolar large rRNA transcription by RNA polymerase I
biological_process	GO:0001188	RNA polymerase I preinitiation complex assembly
biological_process	GO:0006362	transcription elongation from RNA polymerase I promoter
cellular_component	GO:0005736	RNA polymerase I complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity