Lcy02g000320.1 (mRNA) Sponge gourd (P93075) v1

Overview
NameLcy02g000320.1
TypemRNA
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionUPF0496 protein At3g49070
LocationChr02: 604471 .. 605968 (+)
Sequence length1267
RNA-Seq ExpressionLcy02g000320.1
SyntenyLcy02g000320.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAGCTCCAAATTTTGCTGCCTAAATTTCAATTTTCTTTCCTTCAAGCAAACAGCAACAAAATAATCCAAAACCCTTCTTCTTATCTTCTCTCTCTTCCTATAAATTAAAACATCTTCTCTTTCTTTCTCTCTCATTTCCTTCATTTGTTTTTACAGATTATTATTCATGTTTCCAGACGAGATGAAGAAATCAAACATTGTTTCACGTCTCAGAAAATTTCTTTCATGCTCTGGTAATTCAAATCAACCCTGTTTTACTGTTTAAATAAATTCCTTTTCTTTTGTTAAGGGTTAAATATACATGTTCTGGTGTTTTCTTCAATTTGGTCTGTAACTTTTTAACTTTTTATTATAATTATCATTAATTCTGTTCGCTAAAATTAAAATCCATGAAATATAACATCACATCACACTTTATTTTATATAATATAATTTTATTTATATTTTTTTTTGGTGTTGAATAGACGGTGCGGCACACATTACAAGTCATCCAATAGGTGTAGACGTGCGGGAGGAGTACGCTAATGCCTTCCGCACCGAATCATACCTCGACTTTTGGACACGTGTCCTTGCCTTAAATGATGGAGAAGTGTCCGTAGAGTCCACAACCGCAACCCGCCTCTCATCTTACAGGCTATTCGCCGAACATCTACTGGACCCAGCCCAGCCCACAGTTAAAAGAATCCTAACTTCGGCCCATCTTCGGCCCAATTCCCACTCCCTCCTCTCCGATTATTTCTCTCACACCGCCGACGCCTCTCTCTTGTGTGGGCGTTTACTCAACGACGTCGATCATTTACGTCTCAAACTCCGTCCCCTTGAAATTACCCTCCAGTCCCTCGAAAGTACCAAATTTCACCATTTCAAGCCACTCTCGATCAACCCATTCGTATCATCTGCAGAACGAGTTCAAACGATCCAAAACGGGTGCTCCAAATTGTTGAAGCGACTCGAGTTCGAGCGGGACAAGGCTCGTGCCAGGCTGAAGAGAGTCAGATATTTCCAACATGGTTCAGCTGGGCTCTTGGTCGCTATAACGGCGTCGCTCACAGTAATCGTTGTGAGTCATGGAATTGCGTTGCTTGTTGTTGCTGCACCAGGGCTCCTTGTGGGTGCTGTAAGGTTGGCTAAGTCGAGGAAGCTAGCTAAGGAGGTGGCTCGACTCGACGTGGCTGCCAAAGGAACTTACACTTTGAATAGGGATTTGGACACGATTGGTCGGGTCCTGGCTCGGCTCAATCACGAGGTGGAACACATGAGGGTAATGACGAGGTTTTGGCTCGATGGACGCCGAGCCATAGATGAAGTGGCGCGCCACTTGATACAAACTCATCTCAACTTTAGCCAGCAATTGGATGAACTTGAGGAGCATTTGTATTTGTGTTTTATGACCATAAATCGAGCCAGAAATCTTGTAGTCAAAGAGATTTTGGATTCGGCTCAACCAGTAAAGATTCCATATTTATGATTATATAGTAAAACAAAGAGAAGTCTCAA

mRNA sequence

CAAGCTCCAAATTTTGCTGCCTAAATTTCAATTTTCTTTCCTTCAAGCAAACAGCAACAAAATAATCCAAAACCCTTCTTCTTATCTTCTCTCTCTTCCTATAAATTAAAACATCTTCTCTTTCTTTCTCTCTCATTTCCTTCATTTGTTTTTACAGATTATTATTCATGTTTCCAGACGAGATGAAGAAATCAAACATTGTTTCACGTCTCAGAAAATTTCTTTCATGCTCTGACGGTGCGGCACACATTACAAGTCATCCAATAGGTGTAGACGTGCGGGAGGAGTACGCTAATGCCTTCCGCACCGAATCATACCTCGACTTTTGGACACGTGTCCTTGCCTTAAATGATGGAGAAGTGTCCGTAGAGTCCACAACCGCAACCCGCCTCTCATCTTACAGGCTATTCGCCGAACATCTACTGGACCCAGCCCAGCCCACAGTTAAAAGAATCCTAACTTCGGCCCATCTTCGGCCCAATTCCCACTCCCTCCTCTCCGATTATTTCTCTCACACCGCCGACGCCTCTCTCTTGTGTGGGCGTTTACTCAACGACGTCGATCATTTACGTCTCAAACTCCGTCCCCTTGAAATTACCCTCCAGTCCCTCGAAAGTACCAAATTTCACCATTTCAAGCCACTCTCGATCAACCCATTCGTATCATCTGCAGAACGAGTTCAAACGATCCAAAACGGGTGCTCCAAATTGTTGAAGCGACTCGAGTTCGAGCGGGACAAGGCTCGTGCCAGGCTGAAGAGAGTCAGATATTTCCAACATGGTTCAGCTGGGCTCTTGGTCGCTATAACGGCGTCGCTCACAGTAATCGTTGTGAGTCATGGAATTGCGTTGCTTGTTGTTGCTGCACCAGGGCTCCTTGTGGGTGCTGTAAGGTTGGCTAAGTCGAGGAAGCTAGCTAAGGAGGTGGCTCGACTCGACGTGGCTGCCAAAGGAACTTACACTTTGAATAGGGATTTGGACACGATTGGTCGGGTCCTGGCTCGGCTCAATCACGAGGTGGAACACATGAGGGTAATGACGAGGTTTTGGCTCGATGGACGCCGAGCCATAGATGAAGTGGCGCGCCACTTGATACAAACTCATCTCAACTTTAGCCAGCAATTGGATGAACTTGAGGAGCATTTGTATTTGTGTTTTATGACCATAAATCGAGCCAGAAATCTTGTAGTCAAAGAGATTTTGGATTCGGCTCAACCAGTAAAGATTCCATATTTATGATTATATAGTAAAACAAAGAGAAGTCTCAA

Coding sequence (CDS)

ATGTTTCCAGACGAGATGAAGAAATCAAACATTGTTTCACGTCTCAGAAAATTTCTTTCATGCTCTGACGGTGCGGCACACATTACAAGTCATCCAATAGGTGTAGACGTGCGGGAGGAGTACGCTAATGCCTTCCGCACCGAATCATACCTCGACTTTTGGACACGTGTCCTTGCCTTAAATGATGGAGAAGTGTCCGTAGAGTCCACAACCGCAACCCGCCTCTCATCTTACAGGCTATTCGCCGAACATCTACTGGACCCAGCCCAGCCCACAGTTAAAAGAATCCTAACTTCGGCCCATCTTCGGCCCAATTCCCACTCCCTCCTCTCCGATTATTTCTCTCACACCGCCGACGCCTCTCTCTTGTGTGGGCGTTTACTCAACGACGTCGATCATTTACGTCTCAAACTCCGTCCCCTTGAAATTACCCTCCAGTCCCTCGAAAGTACCAAATTTCACCATTTCAAGCCACTCTCGATCAACCCATTCGTATCATCTGCAGAACGAGTTCAAACGATCCAAAACGGGTGCTCCAAATTGTTGAAGCGACTCGAGTTCGAGCGGGACAAGGCTCGTGCCAGGCTGAAGAGAGTCAGATATTTCCAACATGGTTCAGCTGGGCTCTTGGTCGCTATAACGGCGTCGCTCACAGTAATCGTTGTGAGTCATGGAATTGCGTTGCTTGTTGTTGCTGCACCAGGGCTCCTTGTGGGTGCTGTAAGGTTGGCTAAGTCGAGGAAGCTAGCTAAGGAGGTGGCTCGACTCGACGTGGCTGCCAAAGGAACTTACACTTTGAATAGGGATTTGGACACGATTGGTCGGGTCCTGGCTCGGCTCAATCACGAGGTGGAACACATGAGGGTAATGACGAGGTTTTGGCTCGATGGACGCCGAGCCATAGATGAAGTGGCGCGCCACTTGATACAAACTCATCTCAACTTTAGCCAGCAATTGGATGAACTTGAGGAGCATTTGTATTTGTGTTTTATGACCATAAATCGAGCCAGAAATCTTGTAGTCAAAGAGATTTTGGATTCGGCTCAACCAGTAAAGATTCCATATTTATGA

Protein sequence

MFPDEMKKSNIVSRLRKFLSCSDGAAHITSHPIGVDVREEYANAFRTESYLDFWTRVLALNDGEVSVESTTATRLSSYRLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSDYFSHTADASLLCGRLLNDVDHLRLKLRPLEITLQSLESTKFHHFKPLSINPFVSSAERVQTIQNGCSKLLKRLEFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGIALLVVAAPGLLVGAVRLAKSRKLAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWLDGRRAIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVKEILDSAQPVKIPYL
Homology
BLAST of Lcy02g000320.1 vs. ExPASy Swiss-Prot
Match: Q9SMU4 (UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.1e-75
Identity = 177/352 (50.28%), Postives = 225/352 (63.92%), Query Frame = 0

Query: 22  SDGAAHITSHP---IGVDVREEYANAFRTESYLDFWTRVLALNDGEVSV----------E 81
           S G A  +S+P     VDVREEYANAFRTESY  FWTRV+ L+  + +V           
Sbjct: 53  SIGTASGSSNPKDGADVDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIES 112

Query: 82  STTATRLSSYRLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSDYFSHTADASLLCGRLL 141
           S+T+ RL SYRLFA +LLDP   T+ RIL  + +  ++ +LLSDYF  TA+A LLC +LL
Sbjct: 113 SSTSARLMSYRLFAHNLLDPDLNTITRILDVSRVGRHTRTLLSDYFLETANAFLLCTQLL 172

Query: 142 NDVDHLRLKLRPLEITLQSLESTK---FHHFKPLS--INPFVSSAERVQTIQNGCSKLLK 201
            ++ HLR K   L+    S           F  +S   +PF+SS  R+Q I++GC  LLK
Sbjct: 173 KNIHHLRSKYESLKPKFHSENHNSLALIDQFTEISKWFDPFISSGSRIQLIRSGCLYLLK 232

Query: 202 RLEFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGIALLVVAAPGLLVGAVRL 261
           RLE  RDK RA+LK +    H S  L++A+T +L V + SH  AL  +AAP LL    + 
Sbjct: 233 RLESRRDKTRAKLKLINGLTHSSGLLVLALTTTLIVTIASHAFALF-LAAPTLLASQFKP 292

Query: 262 AKSR-KLAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWL---DGR- 321
           A  R KL K  ARLDVAAKGTY L+RDLDTI R++ R+N EV H+R M  FW+    GR 
Sbjct: 293 AGLRNKLTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGSGRV 352

Query: 322 RAIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVKEILDSAQP 351
           R  +EVAR L +   +FS++LDELEEH+YLCFMTINRARNL+VKEILDS  P
Sbjct: 353 RGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEILDSDDP 403

BLAST of Lcy02g000320.1 vs. ExPASy Swiss-Prot
Match: A2XCJ1 (UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 1.4e-49
Identity = 135/346 (39.02%), Postives = 189/346 (54.62%), Query Frame = 0

Query: 36  DVREEYANAFRTESYLDFWTRVL--ALNDGEVSVE-------STTATRLSSYRLFAEHLL 95
           D REEY +AFRTESY DFW RVL   L  G   V           + RL SYRLFAEHLL
Sbjct: 33  DFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFAEHLL 92

Query: 96  DPAQPTVKRILTS---AHLRPNSHSLLSDYFSHTADASLLCGRLLNDVDHLRLKLRPLEI 155
           +P Q  V   L S   + LRP+   LL+ Y++ TA+AS LC  LL D++H+RL+ RPL+ 
Sbjct: 93  EPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYRPLKH 152

Query: 156 TLQSLES----TKFHHFKPLSINPF---VSSAERVQTIQNGCSKLLKRLEFERDKARARL 215
           TL+ L S    +           PF    +S  R++ +Q G   LL+ L+  R KAR R+
Sbjct: 153 TLRKLASDVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLDAGRKKARHRI 212

Query: 216 KRVRYFQHGSAGLLVAITASLTVIVVSHGIALLVVAAPGLLVGAV----RLAKSRKLAKE 275
           + V   +   +   V   A + V+    G+ +L   A   ++       R    R   + 
Sbjct: 213 RSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLGERFFSGRAARRA 272

Query: 276 VARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWLDGR---------RAIDE 335
           + +L+ AAKGTY LNRD++TI R++AR+  E EHM  + R  ++ R         R + E
Sbjct: 273 LVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALLRLCVEHRPAAGAGGKGRLVQE 332

Query: 336 VARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVKEILDSAQ 350
           V R L +   +F QQLDELEEHL+LCFMTIN+AR +V+  +  +A+
Sbjct: 333 VLRQLSKNEESFRQQLDELEEHLFLCFMTINKARIMVMNFMAAAAR 378

BLAST of Lcy02g000320.1 vs. ExPASy Swiss-Prot
Match: Q10RR9 (UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 5.3e-49
Identity = 134/346 (38.73%), Postives = 188/346 (54.34%), Query Frame = 0

Query: 36  DVREEYANAFRTESYLDFWTRVL--ALNDGEVSVE-------STTATRLSSYRLFAEHLL 95
           D REEY +AFRTESY DFW RVL   L  G   V           + RL SYRLFAEHLL
Sbjct: 33  DFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFAEHLL 92

Query: 96  DPAQPTVKRILTS---AHLRPNSHSLLSDYFSHTADASLLCGRLLNDVDHLRLKLRPLEI 155
           +P Q  V   L S   + LRP+   LL+ Y++ TA+AS LC  LL D++H+RL+ RPL+ 
Sbjct: 93  EPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYRPLKH 152

Query: 156 TLQSLES----TKFHHFKPLSINPF---VSSAERVQTIQNGCSKLLKRLEFERDKARARL 215
           TL+ L S    +           PF    +S  R++ +Q G   LL+ L+  R KAR R+
Sbjct: 153 TLRKLASDVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLDAGRKKARHRI 212

Query: 216 KRVRYFQHGSAGLLVAITASLTVIVVSHGIALLVVAAPGLLVGAV----RLAKSRKLAKE 275
           + V   +   +   V   A + V+    G+ +L   A   ++       R    R   + 
Sbjct: 213 RSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLGERFFSGRAARRA 272

Query: 276 VARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWLDGR---------RAIDE 335
           + +L+ AAKGTY LNRD++TI R++AR+  E EHM  + R  ++ R         R + E
Sbjct: 273 LVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALRRLCVEHRPAAGAGGKGRLVQE 332

Query: 336 VARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVKEILDSAQ 350
           V R L +   +F QQLDELEEHL+LCFMT N+AR +V+  +  +A+
Sbjct: 333 VLRQLSKNEESFRQQLDELEEHLFLCFMTTNKARIMVMNFMAAAAR 378

BLAST of Lcy02g000320.1 vs. ExPASy Swiss-Prot
Match: Q9LJK4 (UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 8.6e-23
Identity = 94/333 (28.23%), Postives = 167/333 (50.15%), Query Frame = 0

Query: 36  DVREEYANAFRTESYLDFWTRVLALNDGEVSVESTTATRLSSYRLFAEHLLDPAQPTVKR 95
           ++  E A+AF+T SY D  +R+L ++  + ++E           LF    L P   +V+ 
Sbjct: 35  NLSHELAHAFQTPSYHDIRSRLLVIDPTQENLE-----------LFLSQELRPNNESVQE 94

Query: 96  ILTSAHLRPNS-HSLLSDYFSHTADASLLCGRLLNDVDHLRLKLRPLEITL--------- 155
            L+  H +  +  +L+S YF H+ DA+  C  L  +V   R  L    + L         
Sbjct: 95  ALSLRHAKQTTLTNLVSTYFQHSEDATRFCLNLYQNVHSARCHLYTPLLDLFNIFPRDSH 154

Query: 156 ----QSLESTKFHHFKPLSI--NPFVS-SAERVQTIQNGCSKLLKRLEFERDKARARLKR 215
               +S  +  F  F  L    NPF S  +   Q  Q    +L  +L+    K+++R++ 
Sbjct: 155 SAIDESFCNLAFDVFLKLDTFENPFASPESHSFQDTQLCFYQLADKLDTRIRKSKSRVRL 214

Query: 216 VRYFQHGSAGLL---VAITASLTVIVVSHGIALLVVAAPGLLVGAVRLAKSRKLAKEVAR 275
           + +   GSA  L   V + A+    +  H +  ++V A  L    +  +  +K    + +
Sbjct: 215 LHHATAGSALCLVTAVVVVAASAAFIAYHALPTILVVAGPLCTPYLPHSFKKKELSNIFQ 274

Query: 276 LDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWLDGRR---AIDEVARHLIQTH 335
           L+VAAKGT+ LN+DLDTI R+++RL+  V++ +++ R  L+  R    I E  + L ++H
Sbjct: 275 LNVAAKGTFALNKDLDTIDRLVSRLHTGVKNDKLLIRLGLERGRDVYTIPEFVKQLRKSH 334

Query: 336 LNFSQQLDELEEHLYLCFMTINRARNLVVKEIL 346
           +N + QL+ L +H+   F  +N++R+L++KEIL
Sbjct: 335 VNHTHQLEVLVDHICRWFTNVNKSRSLLLKEIL 356

BLAST of Lcy02g000320.1 vs. ExPASy Swiss-Prot
Match: Q6DYE5 (UPF0496 protein At1g20180 OS=Arabidopsis thaliana OX=3702 GN=At1g20180 PE=2 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 4.3e-22
Identity = 101/356 (28.37%), Postives = 172/356 (48.31%), Query Frame = 0

Query: 35  VDVREEYANAFRTESYLDFWTRV---LALND----GEVSVESTTATRLSSYRLFAEHLLD 94
           + V EEY  AFRT SYL+  T+    L +         S   ++++ LS +  F ++LLD
Sbjct: 34  LSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTDYLLD 93

Query: 95  PAQPTVKRILTSAHLRPNSHSLLSDYFSHTADASLLCGRLLN-----DVDHLRLK----- 154
           P Q T+  ++  + L     +L+  +F  +++A  +C  LL       ++H ++K     
Sbjct: 94  PPQETLDALMQDSSL----DNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVMKI 153

Query: 155 -------LRPLEITLQSLESTKFHHFKPLSI--NPF--VSSAERVQTIQNGCSKLLKRLE 214
                   + LE + + L +  F      +   NP   + +  + + + +  S LL +L 
Sbjct: 154 GKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTKLT 213

Query: 215 FERDKARARLKRVRYFQH-GSAGLLVAITA-SLTVIVVSHGIALLVVAAPGLL------- 274
            ++ + R +++  ++ +  G   L++  +A  +T+++++    L V AAP LL       
Sbjct: 214 SKKRRIRRKIRFFKFCKKLGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCSFCL 273

Query: 275 ----VGAVRLAKSRK---LAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVM 334
                   R+ KS K   L K   ++D+AAKG + L  DLDT+ R+  RL  E+EH + +
Sbjct: 274 LRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRKTV 333

Query: 335 TRFWLDGRR--AIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVKEI 345
                  R+   + E  R        FS QL ELEEHLYLCF TINR+R LV+ +I
Sbjct: 334 AAMCAKSRKIEVLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 385

BLAST of Lcy02g000320.1 vs. ExPASy TrEMBL
Match: A0A6J1DZ81 (UPF0496 protein At3g49070 OS=Momordica charantia OX=3673 GN=LOC111025959 PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 5.6e-142
Identity = 273/374 (72.99%), Postives = 317/374 (84.76%), Query Frame = 0

Query: 6   MKKSNIVSRLRKFLSCSDGAAHITSHPIGVDVREEYANAFRTESYLDFWTRVLALNDGEV 65
           M+K  IVSRL KFLSCSDG  HITS+PIGVDVREEYANAFRTESY+DFWTRVL LNDG++
Sbjct: 1   MRKFKIVSRLIKFLSCSDGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGDL 60

Query: 66  S----VESTTATRLSSYRLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSDYFSHTADAS 125
           +    VESTTATRLSSYRLFAEHLLDP QPTVKR+L S +LRPNS+SLL+DYFSHTA+AS
Sbjct: 61  TTQLPVESTTATRLSSYRLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANAS 120

Query: 126 LLCGRLLNDVDHLRLKLRPLEITLQSLESTKFHH---FKPL---------SINPFVSSA- 185
           LLCGRLL D+ +LRLKLRPL+ITL S+E+T+F+H   F+P+         + NPFVSSA 
Sbjct: 121 LLCGRLLKDIHNLRLKLRPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAP 180

Query: 186 --ERVQTIQNGCSKLLKRLEFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGI 245
              R++ IQ+GCS LLK+LEF RDKARA+L+RVRYFQHGSAG LVA+T SLTVIV++HGI
Sbjct: 181 STRRIRIIQSGCSNLLKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGI 240

Query: 246 ALLVVAAPGLLVGAVRLAKSRKLAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEH 305
           AL  VAAPGLLV ++ LAKSRKLAK+VA+L+VAAKGTYTLNRD DT+GR++ARL+HE+EH
Sbjct: 241 ALF-VAAPGLLVASIELAKSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEH 300

Query: 306 MRVMTRFWL----DGRRAIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVK 357
           MRVMTRFWL    D R+AI EVAR L Q H +FSQQLDELEEHLYLCFMTINRARNLVVK
Sbjct: 301 MRVMTRFWLERREDRRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVK 360

BLAST of Lcy02g000320.1 vs. ExPASy TrEMBL
Match: A0A1S3ATJ2 (UPF0496 protein At3g49070 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482559 PE=3 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.4e-124
Identity = 256/374 (68.45%), Postives = 295/374 (78.88%), Query Frame = 0

Query: 6   MKKSNIVSRLRKFLSCSDGAAHITSHPIGVDVREEYANAFRTESYLDFWTRVLALNDG-- 65
           MKK  I+S LRKFLSCSDG  H+T++P+  DV EEYANAFRTESY+DFWTRV+ALN+G  
Sbjct: 1   MKKLKIISCLRKFLSCSDGEQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60

Query: 66  ---EVSVESTTATRLSSYRLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSD-YFSHTAD 125
              +VS+ESTTATRLSSYRLF EHLLDP QPT+KR+LT AHL PNS SLL D YFSHT++
Sbjct: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKRMLT-AHLGPNSCSLLLDHYFSHTSN 120

Query: 126 ASLLCGRLLNDVDHLRLKLRPLEITLQSLESTKFHHFKPLSI-----------NPFVS-S 185
           ASLLC R+L  + HLRLKLR L+   Q        HFK L +           N FV   
Sbjct: 121 ASLLCSRILKHIVHLRLKLRSLDQNKQEFNYDD-SHFKQLLVCLVEFSNNSTPNSFVPYC 180

Query: 186 AERVQTIQNGCSKLLKRLEFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGIA 245
            E+VQ IQNGCSKLLKRLE+ RDKA+ +LKRVRYFQH SAG LVAITAS T+IVV+HGIA
Sbjct: 181 MEQVQIIQNGCSKLLKRLEYSRDKAQDKLKRVRYFQHSSAGFLVAITASFTIIVVTHGIA 240

Query: 246 LLVVAAPGLLVGAVRLA-KSRKLAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEH 305
           L  VAAPG LVGA++L  KSRKLAK+VA+L+VAAKGTYTLNRD DTIGR++ARL+HE+EH
Sbjct: 241 LF-VAAPGFLVGAIKLVKKSRKLAKKVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEH 300

Query: 306 MRVMTRFWL----DGRRAIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVK 357
           MRVMT+FWL    D R AI E+ R L Q+H NF+QQLDELEEHLYLCFMTINRARNLVVK
Sbjct: 301 MRVMTKFWLDRGEDKRWAIGELLRQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVK 360

BLAST of Lcy02g000320.1 vs. ExPASy TrEMBL
Match: A0A6J1JZN7 (UPF0496 protein At3g49070 OS=Cucurbita maxima OX=3661 GN=LOC111491151 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 3.1e-116
Identity = 250/355 (70.42%), Postives = 282/355 (79.44%), Query Frame = 0

Query: 6   MKKSNIVSRLRKFLSCSDGAAHITSHPIGVDVREEYANAFRTESYLDFWTRVLALNDGEV 65
           MKKS +VSRLRK  S SDG  HI S PIGVDVREEYANAFRTESYLDFWTRV+AL D  V
Sbjct: 1   MKKSKLVSRLRKLRSSSDGGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVALKD--V 60

Query: 66  SVESTTATRLSSYRLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSDYFSHTADASLLCG 125
           +VESTTA+RLSSYRLFAEHLLDP +PTVKRIL  AHLRPNS SLLSDYFSHTA+ASLLC 
Sbjct: 61  AVESTTASRLSSYRLFAEHLLDPNEPTVKRILNWAHLRPNSKSLLSDYFSHTANASLLCS 120

Query: 126 RLLNDVDHLRLKLRPLEITLQSLESTKFHHFKPLSI-NPFVSSAERVQTIQNGCSKLLKR 185
           RLL D++HLR      EI +QSL++ +F +FKPLSI NPF       + IQNGC+KLLK+
Sbjct: 121 RLLKDINHLR-----PEIAIQSLQNPEF-NFKPLSIHNPF------PEIIQNGCAKLLKQ 180

Query: 186 LEFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGIALLVV-AAPGL--LVGAV 245
           LEF RDKAR ++KRVRYFQHGSAGLLVA+TASLTVI+V+HGI L+VV AAP L  LVGAV
Sbjct: 181 LEFNRDKARTKVKRVRYFQHGSAGLLVAVTASLTVILVTHGIGLVVVAAAPCLAGLVGAV 240

Query: 246 RLAKSRKLAKEVAR-LDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWL----- 305
           +LA+     KEVAR LDVAAK TYTLNRD DT+GR++ARLN EVEHMR + RFW+     
Sbjct: 241 KLAR----VKEVARVLDVAAKATYTLNRDFDTVGRLVARLNQEVEHMRGLMRFWVERGEG 300

Query: 306 -DGRR-AIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVKEILDSA 349
            DGR   + EVAR L Q  LN  Q LDELEE LYLCFMTINRARNLV+K+IL  A
Sbjct: 301 RDGRHGGVGEVARQLQQCRLNLGQHLDELEELLYLCFMTINRARNLVLKQILARA 337

BLAST of Lcy02g000320.1 vs. ExPASy TrEMBL
Match: A0A0A0KN20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166480 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 4.0e-116
Identity = 241/356 (67.70%), Postives = 277/356 (77.81%), Query Frame = 0

Query: 24  GAAHITSHPIGVDVREEYANAFRTESYLDFWTRVLALNDG-----EVSVESTTATRLSSY 83
           G  H+ ++P+  DV EEYANAFRTESY+DFWTRV+ALN+G     +VS+ESTTATRLSSY
Sbjct: 4   GGQHVINYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLSSY 63

Query: 84  RLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSD-YFSHTADASLLCGRLLNDVDHLRLK 143
           RLF EHLLDP QPT+K ILT AHL PNS SLL D YFSHTA+ASLLC R+L  + HLRLK
Sbjct: 64  RLFVEHLLDPPQPTIKTILT-AHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLK 123

Query: 144 LRPLEITLQSLESTKFHHFKPLSINPFVSS------------AERVQTIQNGCSKLLKRL 203
           L  L+   Q        HFK L +  F  S             E+VQ IQNGCSKLLKRL
Sbjct: 124 LHSLDQNKQEFNHDD-SHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCSKLLKRL 183

Query: 204 EFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGIALLVVAAPGLLVGAVRLA- 263
           E+ RDK R +LKRVRYFQH SAG LVAITAS TVIVV+HGIAL  VAAPG LVGA++LA 
Sbjct: 184 EYSRDKTRDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALF-VAAPGFLVGAIKLAK 243

Query: 264 KSRKLAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWL----DGRRA 323
           KSRKLAKEVA+L+VAAKGTYTLNRD DTIGR++ARL+HE+EHM+VM +FWL    D R A
Sbjct: 244 KSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDKGGDKRWA 303

Query: 324 IDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVKEILDSAQPVKIPYL 357
           IDE+AR L Q+H NF+QQLDELEEHLYLCFMTINRARNLVVKEIL+ ++P+KI YL
Sbjct: 304 IDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNLSKPIKISYL 356

BLAST of Lcy02g000320.1 vs. ExPASy TrEMBL
Match: A0A6J1H2B8 (UPF0496 protein At3g49070-like OS=Cucurbita moschata OX=3662 GN=LOC111459391 PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 7.6e-115
Identity = 248/352 (70.45%), Postives = 280/352 (79.55%), Query Frame = 0

Query: 6   MKKSNIVSRLRKFLSCSDGAAHITSHPIGVDVREEYANAFRTESYLDFWTRVLALNDGEV 65
           MKKS +VSRLR FL  SDG  HI S PIGVDVREEYANAFRTESYLDFWTRV+AL D  V
Sbjct: 1   MKKSKLVSRLRTFL--SDGGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVALKD--V 60

Query: 66  SVESTTATRLSSYRLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSDYFSHTADASLLCG 125
            VESTTA+RLSSYRLFAEHLLDP +PTVKRIL  AHLRPN  SLLSDYFSHTA+ASLLC 
Sbjct: 61  VVESTTASRLSSYRLFAEHLLDPTEPTVKRILNWAHLRPNFKSLLSDYFSHTANASLLCS 120

Query: 126 RLLNDVDHLRLKLRPLEITLQSLESTKFHHFKPLSI-NPFVSSAERVQTIQNGCSKLLKR 185
           RLL D++HLR      EI +QSL++ +F +FKPLSI NPF +       IQNGC+KLLK+
Sbjct: 121 RLLKDMNHLR-----PEIAIQSLQNPEF-NFKPLSIHNPFPA------IIQNGCAKLLKQ 180

Query: 186 LEFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGIALLVV-AAPGL--LVGAV 245
           LEF RDKAR ++KRVRYFQH SAGLLVA+TASLTVI+V+HGI L+VV AAPGL  LVGAV
Sbjct: 181 LEFNRDKARTKVKRVRYFQHSSAGLLVAVTASLTVILVTHGIGLVVVAAAPGLVGLVGAV 240

Query: 246 RLAKSRKLAKEVAR-LDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWL----- 305
           +LA+     KEVAR LDVAAK TYTLNRD DT+GR++ARLN EVEHM+ + RFW+     
Sbjct: 241 KLAR----VKEVARVLDVAAKATYTLNRDFDTVGRLVARLNQEVEHMKGLMRFWVERGEG 300

Query: 306 -DGRR-AIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVKEIL 346
            DGR   + EVAR L Q  LN  Q LDELEEHLYLCFMTINRARNLV+K+IL
Sbjct: 301 RDGRHGGVGEVARQLQQCRLNLGQHLDELEEHLYLCFMTINRARNLVLKQIL 332

BLAST of Lcy02g000320.1 vs. NCBI nr
Match: XP_022159588.1 (UPF0496 protein At3g49070 [Momordica charantia])

HSP 1 Score: 513.8 bits (1322), Expect = 1.2e-141
Identity = 273/374 (72.99%), Postives = 317/374 (84.76%), Query Frame = 0

Query: 6   MKKSNIVSRLRKFLSCSDGAAHITSHPIGVDVREEYANAFRTESYLDFWTRVLALNDGEV 65
           M+K  IVSRL KFLSCSDG  HITS+PIGVDVREEYANAFRTESY+DFWTRVL LNDG++
Sbjct: 1   MRKFKIVSRLIKFLSCSDGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGDL 60

Query: 66  S----VESTTATRLSSYRLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSDYFSHTADAS 125
           +    VESTTATRLSSYRLFAEHLLDP QPTVKR+L S +LRPNS+SLL+DYFSHTA+AS
Sbjct: 61  TTQLPVESTTATRLSSYRLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANAS 120

Query: 126 LLCGRLLNDVDHLRLKLRPLEITLQSLESTKFHH---FKPL---------SINPFVSSA- 185
           LLCGRLL D+ +LRLKLRPL+ITL S+E+T+F+H   F+P+         + NPFVSSA 
Sbjct: 121 LLCGRLLKDIHNLRLKLRPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAP 180

Query: 186 --ERVQTIQNGCSKLLKRLEFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGI 245
              R++ IQ+GCS LLK+LEF RDKARA+L+RVRYFQHGSAG LVA+T SLTVIV++HGI
Sbjct: 181 STRRIRIIQSGCSNLLKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGI 240

Query: 246 ALLVVAAPGLLVGAVRLAKSRKLAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEH 305
           AL  VAAPGLLV ++ LAKSRKLAK+VA+L+VAAKGTYTLNRD DT+GR++ARL+HE+EH
Sbjct: 241 ALF-VAAPGLLVASIELAKSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEH 300

Query: 306 MRVMTRFWL----DGRRAIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVK 357
           MRVMTRFWL    D R+AI EVAR L Q H +FSQQLDELEEHLYLCFMTINRARNLVVK
Sbjct: 301 MRVMTRFWLERREDRRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVK 360

BLAST of Lcy02g000320.1 vs. NCBI nr
Match: XP_038875828.1 (UPF0496 protein At3g49070-like [Benincasa hispida])

HSP 1 Score: 498.8 bits (1283), Expect = 3.8e-137
Identity = 271/374 (72.46%), Postives = 303/374 (81.02%), Query Frame = 0

Query: 6   MKKSNIVSRLRKFLSCSDGAAHITSHPIGVDVREEYANAFRTESYLDFWTRVLALNDG-- 65
           MKKS IVS LRKFLSCSDG  H+TSH + +DVREEYANAFRTESY+DFWTRVLALN+G  
Sbjct: 1   MKKSKIVSCLRKFLSCSDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDD 60

Query: 66  ---EVSVESTTATRLSSYRLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSDYFSHTADA 125
              ++SVESTTAT LSSYRLF EHLLDP Q T+KRILTS HL  NS SLL DYFSHTA+A
Sbjct: 61  LTTQLSVESTTATHLSSYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANA 120

Query: 126 SLLCGRLLNDVDHLRLKLRPLEITLQSLES-TKFH---HFKPLSI---------NPFVSS 185
           SL C  LL  ++HLRLKL PLEITLQSLE+  KFH   HFK L I         NPF+ S
Sbjct: 121 SLSCSHLLKHINHLRLKLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFIPS 180

Query: 186 AERVQTIQNGCSKLLKRLEFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGIA 245
            E+VQ IQNGCSKLLK+LEF RDKA+ +LKRVRYFQH SAG LVA+TASLTVI+V+HGIA
Sbjct: 181 IEQVQIIQNGCSKLLKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIA 240

Query: 246 LLVVAAPGLLVGAVRLAKS-RKLAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEH 305
           L  VAAPG LVGA++LA   RKL K+VARL++ AKGTYTLNRD DTIGR++ARLNHE+EH
Sbjct: 241 LF-VAAPGFLVGAIKLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEH 300

Query: 306 MRVMTRFWL----DGRRAIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVK 357
           MRVM RFWL    D RRAI E+ R L Q+H+NFS QLDELEEHLYLCFMTINRARNLVVK
Sbjct: 301 MRVMARFWLDRGEDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVK 360

BLAST of Lcy02g000320.1 vs. NCBI nr
Match: XP_008436996.1 (PREDICTED: UPF0496 protein At3g49070 isoform X1 [Cucumis melo])

HSP 1 Score: 456.1 bits (1172), Expect = 2.8e-124
Identity = 256/374 (68.45%), Postives = 295/374 (78.88%), Query Frame = 0

Query: 6   MKKSNIVSRLRKFLSCSDGAAHITSHPIGVDVREEYANAFRTESYLDFWTRVLALNDG-- 65
           MKK  I+S LRKFLSCSDG  H+T++P+  DV EEYANAFRTESY+DFWTRV+ALN+G  
Sbjct: 1   MKKLKIISCLRKFLSCSDGEQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60

Query: 66  ---EVSVESTTATRLSSYRLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSD-YFSHTAD 125
              +VS+ESTTATRLSSYRLF EHLLDP QPT+KR+LT AHL PNS SLL D YFSHT++
Sbjct: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKRMLT-AHLGPNSCSLLLDHYFSHTSN 120

Query: 126 ASLLCGRLLNDVDHLRLKLRPLEITLQSLESTKFHHFKPLSI-----------NPFVS-S 185
           ASLLC R+L  + HLRLKLR L+   Q        HFK L +           N FV   
Sbjct: 121 ASLLCSRILKHIVHLRLKLRSLDQNKQEFNYDD-SHFKQLLVCLVEFSNNSTPNSFVPYC 180

Query: 186 AERVQTIQNGCSKLLKRLEFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGIA 245
            E+VQ IQNGCSKLLKRLE+ RDKA+ +LKRVRYFQH SAG LVAITAS T+IVV+HGIA
Sbjct: 181 MEQVQIIQNGCSKLLKRLEYSRDKAQDKLKRVRYFQHSSAGFLVAITASFTIIVVTHGIA 240

Query: 246 LLVVAAPGLLVGAVRLA-KSRKLAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEH 305
           L  VAAPG LVGA++L  KSRKLAK+VA+L+VAAKGTYTLNRD DTIGR++ARL+HE+EH
Sbjct: 241 LF-VAAPGFLVGAIKLVKKSRKLAKKVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEH 300

Query: 306 MRVMTRFWL----DGRRAIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVK 357
           MRVMT+FWL    D R AI E+ R L Q+H NF+QQLDELEEHLYLCFMTINRARNLVVK
Sbjct: 301 MRVMTKFWLDRGEDKRWAIGELLRQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVK 360

BLAST of Lcy02g000320.1 vs. NCBI nr
Match: XP_031741820.1 (UPF0496 protein At3g49070 [Cucumis sativus] >KAE8647914.1 hypothetical protein Csa_000030 [Cucumis sativus])

HSP 1 Score: 453.8 bits (1166), Expect = 1.4e-123
Identity = 254/374 (67.91%), Postives = 292/374 (78.07%), Query Frame = 0

Query: 6   MKKSNIVSRLRKFLSCSDGAAHITSHPIGVDVREEYANAFRTESYLDFWTRVLALNDG-- 65
           MKK  I+S L+KFLSCSDG  H+ ++P+  DV EEYANAFRTESY+DFWTRV+ALN+G  
Sbjct: 1   MKKLKIISCLKKFLSCSDGGQHVINYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDN 60

Query: 66  ---EVSVESTTATRLSSYRLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSD-YFSHTAD 125
              +VS+ESTTATRLSSYRLF EHLLDP QPT+K ILT AHL PNS SLL D YFSHTA+
Sbjct: 61  LTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKTILT-AHLGPNSCSLLLDHYFSHTAN 120

Query: 126 ASLLCGRLLNDVDHLRLKLRPLEITLQSLESTKFHHFKPLSINPFVSS------------ 185
           ASLLC R+L  + HLRLKL  L+   Q        HFK L +  F  S            
Sbjct: 121 ASLLCSRILKQIVHLRLKLHSLDQNKQEFNHDD-SHFKQLLVRLFEFSNDSTPNSFVPYC 180

Query: 186 AERVQTIQNGCSKLLKRLEFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGIA 245
            E+VQ IQNGCSKLLKRLE+ RDK R +LKRVRYFQH SAG LVAITAS TVIVV+HGIA
Sbjct: 181 MEQVQIIQNGCSKLLKRLEYSRDKTRDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIA 240

Query: 246 LLVVAAPGLLVGAVRLA-KSRKLAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEH 305
           L  VAAPG LVGA++LA KSRKLAKEVA+L+VAAKGTYTLNRD DTIGR++ARL+HE+EH
Sbjct: 241 LF-VAAPGFLVGAIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEH 300

Query: 306 MRVMTRFWL----DGRRAIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVK 357
           M+VM +FWL    D R AIDE+AR L Q+H NF+QQLDELEEHLYLCFMTINRARNLVVK
Sbjct: 301 MKVMAKFWLDKGGDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVK 360

BLAST of Lcy02g000320.1 vs. NCBI nr
Match: XP_023534108.1 (UPF0496 protein At3g49070-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 430.3 bits (1105), Expect = 1.7e-116
Identity = 250/355 (70.42%), Postives = 283/355 (79.72%), Query Frame = 0

Query: 6   MKKSNIVSRLRKFLSCSDGAAHITSHPIGVDVREEYANAFRTESYLDFWTRVLALNDGEV 65
           MKKS +VSRLRKFL  SDG  HI S PIGVDVREEYANAFRTESYLDFWTRV+A+ D  V
Sbjct: 1   MKKSKLVSRLRKFL--SDGGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVAIKD--V 60

Query: 66  SVESTTATRLSSYRLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSDYFSHTADASLLCG 125
            VESTTA+RLSSYRLFAEHLLDP +PTVKRIL  AHLRPNS SLLSDYFSHTA+ASLLC 
Sbjct: 61  VVESTTASRLSSYRLFAEHLLDPTEPTVKRILNWAHLRPNSKSLLSDYFSHTANASLLCS 120

Query: 126 RLLNDVDHLRLKLRPLEITLQSLESTKFHHFKPLSI-NPFVSSAERVQTIQNGCSKLLKR 185
           RLL D++HLR      EI +QSL++ +F +FKPLSI NPF       + IQNGC+KLLK+
Sbjct: 121 RLLKDINHLR-----PEIAIQSLQNPEF-NFKPLSIHNPF------PEIIQNGCAKLLKQ 180

Query: 186 LEFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGIALLVV-AAPGL--LVGAV 245
           LEF RDKAR ++KRVRYFQH SAGLLVA+TASLTVI+V+HGI L+VV AAPGL  LVGAV
Sbjct: 181 LEFNRDKARTKVKRVRYFQHSSAGLLVAVTASLTVILVTHGIGLVVVAAAPGLAGLVGAV 240

Query: 246 RLAKSRKLAKEVAR-LDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWL----- 305
           +LA+     KE+AR LDVAAK TYTLNRD DT+GR++ARLN EVEHMR + RFW+     
Sbjct: 241 KLAR----VKEMARVLDVAAKATYTLNRDFDTVGRLVARLNQEVEHMRGLMRFWVELGEG 300

Query: 306 -DGRR-AIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVKEILDSA 349
            DGR   + EVAR L Q  LN  Q LDELEEHLYLCFMTINRARNLV+K+IL  A
Sbjct: 301 RDGRHGGVGEVARQLQQCRLNLGQHLDELEEHLYLCFMTINRARNLVLKQILTRA 335

BLAST of Lcy02g000320.1 vs. TAIR 10
Match: AT3G49070.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 285.0 bits (728), Expect = 8.1e-77
Identity = 177/352 (50.28%), Postives = 225/352 (63.92%), Query Frame = 0

Query: 22  SDGAAHITSHP---IGVDVREEYANAFRTESYLDFWTRVLALNDGEVSV----------E 81
           S G A  +S+P     VDVREEYANAFRTESY  FWTRV+ L+  + +V           
Sbjct: 53  SIGTASGSSNPKDGADVDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIES 112

Query: 82  STTATRLSSYRLFAEHLLDPAQPTVKRILTSAHLRPNSHSLLSDYFSHTADASLLCGRLL 141
           S+T+ RL SYRLFA +LLDP   T+ RIL  + +  ++ +LLSDYF  TA+A LLC +LL
Sbjct: 113 SSTSARLMSYRLFAHNLLDPDLNTITRILDVSRVGRHTRTLLSDYFLETANAFLLCTQLL 172

Query: 142 NDVDHLRLKLRPLEITLQSLESTK---FHHFKPLS--INPFVSSAERVQTIQNGCSKLLK 201
            ++ HLR K   L+    S           F  +S   +PF+SS  R+Q I++GC  LLK
Sbjct: 173 KNIHHLRSKYESLKPKFHSENHNSLALIDQFTEISKWFDPFISSGSRIQLIRSGCLYLLK 232

Query: 202 RLEFERDKARARLKRVRYFQHGSAGLLVAITASLTVIVVSHGIALLVVAAPGLLVGAVRL 261
           RLE  RDK RA+LK +    H S  L++A+T +L V + SH  AL  +AAP LL    + 
Sbjct: 233 RLESRRDKTRAKLKLINGLTHSSGLLVLALTTTLIVTIASHAFALF-LAAPTLLASQFKP 292

Query: 262 AKSR-KLAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWL---DGR- 321
           A  R KL K  ARLDVAAKGTY L+RDLDTI R++ R+N EV H+R M  FW+    GR 
Sbjct: 293 AGLRNKLTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGSGRV 352

Query: 322 RAIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVKEILDSAQP 351
           R  +EVAR L +   +FS++LDELEEH+YLCFMTINRARNL+VKEILDS  P
Sbjct: 353 RGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEILDSDDP 403

BLAST of Lcy02g000320.1 vs. TAIR 10
Match: AT3G19250.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 109.4 bits (272), Expect = 6.1e-24
Identity = 94/333 (28.23%), Postives = 167/333 (50.15%), Query Frame = 0

Query: 36  DVREEYANAFRTESYLDFWTRVLALNDGEVSVESTTATRLSSYRLFAEHLLDPAQPTVKR 95
           ++  E A+AF+T SY D  +R+L ++  + ++E           LF    L P   +V+ 
Sbjct: 35  NLSHELAHAFQTPSYHDIRSRLLVIDPTQENLE-----------LFLSQELRPNNESVQE 94

Query: 96  ILTSAHLRPNS-HSLLSDYFSHTADASLLCGRLLNDVDHLRLKLRPLEITL--------- 155
            L+  H +  +  +L+S YF H+ DA+  C  L  +V   R  L    + L         
Sbjct: 95  ALSLRHAKQTTLTNLVSTYFQHSEDATRFCLNLYQNVHSARCHLYTPLLDLFNIFPRDSH 154

Query: 156 ----QSLESTKFHHFKPLSI--NPFVS-SAERVQTIQNGCSKLLKRLEFERDKARARLKR 215
               +S  +  F  F  L    NPF S  +   Q  Q    +L  +L+    K+++R++ 
Sbjct: 155 SAIDESFCNLAFDVFLKLDTFENPFASPESHSFQDTQLCFYQLADKLDTRIRKSKSRVRL 214

Query: 216 VRYFQHGSAGLL---VAITASLTVIVVSHGIALLVVAAPGLLVGAVRLAKSRKLAKEVAR 275
           + +   GSA  L   V + A+    +  H +  ++V A  L    +  +  +K    + +
Sbjct: 215 LHHATAGSALCLVTAVVVVAASAAFIAYHALPTILVVAGPLCTPYLPHSFKKKELSNIFQ 274

Query: 276 LDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWLDGRR---AIDEVARHLIQTH 335
           L+VAAKGT+ LN+DLDTI R+++RL+  V++ +++ R  L+  R    I E  + L ++H
Sbjct: 275 LNVAAKGTFALNKDLDTIDRLVSRLHTGVKNDKLLIRLGLERGRDVYTIPEFVKQLRKSH 334

Query: 336 LNFSQQLDELEEHLYLCFMTINRARNLVVKEIL 346
           +N + QL+ L +H+   F  +N++R+L++KEIL
Sbjct: 335 VNHTHQLEVLVDHICRWFTNVNKSRSLLLKEIL 356

BLAST of Lcy02g000320.1 vs. TAIR 10
Match: AT1G20180.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 107.1 bits (266), Expect = 3.0e-23
Identity = 101/356 (28.37%), Postives = 172/356 (48.31%), Query Frame = 0

Query: 35  VDVREEYANAFRTESYLDFWTRV---LALND----GEVSVESTTATRLSSYRLFAEHLLD 94
           + V EEY  AFRT SYL+  T+    L +         S   ++++ LS +  F ++LLD
Sbjct: 34  LSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTDYLLD 93

Query: 95  PAQPTVKRILTSAHLRPNSHSLLSDYFSHTADASLLCGRLLN-----DVDHLRLK----- 154
           P Q T+  ++  + L     +L+  +F  +++A  +C  LL       ++H ++K     
Sbjct: 94  PPQETLDALMQDSSL----DNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVMKI 153

Query: 155 -------LRPLEITLQSLESTKFHHFKPLSI--NPF--VSSAERVQTIQNGCSKLLKRLE 214
                   + LE + + L +  F      +   NP   + +  + + + +  S LL +L 
Sbjct: 154 GKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTKLT 213

Query: 215 FERDKARARLKRVRYFQH-GSAGLLVAITA-SLTVIVVSHGIALLVVAAPGLL------- 274
            ++ + R +++  ++ +  G   L++  +A  +T+++++    L V AAP LL       
Sbjct: 214 SKKRRIRRKIRFFKFCKKLGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCSFCL 273

Query: 275 ----VGAVRLAKSRK---LAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVM 334
                   R+ KS K   L K   ++D+AAKG + L  DLDT+ R+  RL  E+EH + +
Sbjct: 274 LRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRKTV 333

Query: 335 TRFWLDGRR--AIDEVARHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVKEI 345
                  R+   + E  R        FS QL ELEEHLYLCF TINR+R LV+ +I
Sbjct: 334 AAMCAKSRKIEVLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 385

BLAST of Lcy02g000320.1 vs. TAIR 10
Match: AT3G19330.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 107.1 bits (266), Expect = 3.0e-23
Identity = 98/332 (29.52%), Postives = 167/332 (50.30%), Query Frame = 0

Query: 36  DVREEYANAFRTESYLDFWTRVLALNDGEVSVESTTATRLSSYRLFAEHLLDPAQPTVKR 95
           ++  E A+AF+T SY D  +RV  + D     +           L    +L P +  V+ 
Sbjct: 46  NLSRELAHAFQTPSYHDVRSRVHVVVD---LTQIHHRLIQPDIELLLSQVLQPNKECVQE 105

Query: 96  ILTSAHLRPNS-HSLLSDYFSHTADASLLCGRLLNDVDHLRLKLRPLEITL--------- 155
            +   H++  +  +L+S YF H+ DA+ LC  L  +V   R  L    + L         
Sbjct: 106 AI--RHVKQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPGDSL 165

Query: 156 ----QSLESTKFHHFKPLSI--NPFVS-SAERVQTIQNGCSKLLKRLEFERDKARARLKR 215
               +SL    F  F  L    NPF S  +   +  Q   S+L   L+    K+R+R++ 
Sbjct: 166 PAIDESLCDLAFDVFLKLDTFENPFSSPESYSFRDTQLCFSQLKHNLDRRLRKSRSRVRL 225

Query: 216 VRYFQHGSAGLLVA---ITASLTVIVVSHGIALLVVAAPGLLVGAVRLAKSRKLAKEVAR 275
           + +   GS+  LVA     A+  V++ SH + +L+V A  L    +  +  RK    + +
Sbjct: 226 IHHATAGSSLCLVAAVVAVAASAVVIASHALPILLVVAGPLCSPYLPHSFKRKELTNICQ 285

Query: 276 LDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWLDGRR---AIDEVARHLIQTH 335
           L+ A+KGT+ LN+DLDTI R+++RL+  +E+ + + R  L+  R   +I E+ + L ++H
Sbjct: 286 LNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLGLERGRDVHSIQEILKLLRKSH 345

Query: 336 LNFSQQLDELEEHLYLCFMTINRARNLVVKEI 345
           L  + QL +LE+H+ L F  +N+AR+L++ EI
Sbjct: 346 LPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 372

BLAST of Lcy02g000320.1 vs. TAIR 10
Match: AT1G20180.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 102.1 bits (253), Expect = 9.7e-22
Identity = 99/339 (29.20%), Postives = 155/339 (45.72%), Query Frame = 0

Query: 35  VDVREEYANAFRTESYLDFWTRV---LALND----GEVSVESTTATRLSSYRLFAEHLLD 94
           + V EEY  AFRT SYL+  T+    L +         S   ++++ LS +  F ++LLD
Sbjct: 34  LSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTDYLLD 93

Query: 95  PAQPTVKRILTSAHLRPNSHSLLSDYFSHTADASLLCGRLLN-----DVDHLRLKLRPLE 154
           P Q T+  ++  + L     +L+  +F  +++A  +C  LL       ++H ++K R ++
Sbjct: 94  PPQETLDALMQDSSL----DNLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIK-RVMK 153

Query: 155 ITLQSLESTKFHHFKPLSINPFV-SSAERVQTIQNGCSKLLKRLEFERDKARARLKRVRY 214
           I  +     K     P  +   +     R   ++N   +++   +F            R 
Sbjct: 154 IGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQF------------RI 213

Query: 215 FQHGSAGLLVAITASLTVIVVSHGIALLVVAAPGLL-----------VGAVRLAKSRK-- 274
               ++ LL  +T+    I     I L V AAP LL               R+ KS K  
Sbjct: 214 VHDANSDLLTKLTSKKRRIRRKISI-LGVFAAPALLGLCSFCLLRKKKAKGRMHKSNKDT 273

Query: 275 -LAKEVARLDVAAKGTYTLNRDLDTIGRVLARLNHEVEHMRVMTRFWLDGRR--AIDEVA 334
            L K   ++D+AAKG + L  DLDT+ R+  RL  E+EH + +       R+   + E  
Sbjct: 274 TLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRKTVAAMCAKSRKIEVLKEAL 333

Query: 335 RHLIQTHLNFSQQLDELEEHLYLCFMTINRARNLVVKEI 345
           R        FS QL ELEEHLYLCF TINR+R LV+ +I
Sbjct: 334 REFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SMU41.1e-7550.28UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1[more]
A2XCJ11.4e-4939.02UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2[more]
Q10RR95.3e-4938.73UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 ... [more]
Q9LJK48.6e-2328.23UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1[more]
Q6DYE54.3e-2228.37UPF0496 protein At1g20180 OS=Arabidopsis thaliana OX=3702 GN=At1g20180 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1DZ815.6e-14272.99UPF0496 protein At3g49070 OS=Momordica charantia OX=3673 GN=LOC111025959 PE=3 SV... [more]
A0A1S3ATJ21.4e-12468.45UPF0496 protein At3g49070 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482559 PE=... [more]
A0A6J1JZN73.1e-11670.42UPF0496 protein At3g49070 OS=Cucurbita maxima OX=3661 GN=LOC111491151 PE=3 SV=1[more]
A0A0A0KN204.0e-11667.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166480 PE=3 SV=1[more]
A0A6J1H2B87.6e-11570.45UPF0496 protein At3g49070-like OS=Cucurbita moschata OX=3662 GN=LOC111459391 PE=... [more]
Match NameE-valueIdentityDescription
XP_022159588.11.2e-14172.99UPF0496 protein At3g49070 [Momordica charantia][more]
XP_038875828.13.8e-13772.46UPF0496 protein At3g49070-like [Benincasa hispida][more]
XP_008436996.12.8e-12468.45PREDICTED: UPF0496 protein At3g49070 isoform X1 [Cucumis melo][more]
XP_031741820.11.4e-12367.91UPF0496 protein At3g49070 [Cucumis sativus] >KAE8647914.1 hypothetical protein C... [more]
XP_023534108.11.7e-11670.42UPF0496 protein At3g49070-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G49070.18.1e-7750.28Protein of unknown function (DUF677) [more]
AT3G19250.16.1e-2428.23Protein of unknown function (DUF677) [more]
AT1G20180.13.0e-2328.37Protein of unknown function (DUF677) [more]
AT3G19330.13.0e-2329.52Protein of unknown function (DUF677) [more]
AT1G20180.29.7e-2229.20Protein of unknown function (DUF677) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007749Protein of unknown function DUF677PANTHERPTHR31113UPF0496 PROTEIN 3-RELATEDcoord: 6..349
NoneNo IPR availablePANTHERPTHR31113:SF6UPF0496 PROTEIN 3coord: 6..349

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lcy02g000320Lcy02g000320gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy02g000320.1.utr5p1Lcy02g000320.1.utr5p1five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy02g000320.1.exon1Lcy02g000320.1.exon1exon
Lcy02g000320.1.exon2Lcy02g000320.1.exon2exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy02g000320.1.cds1Lcy02g000320.1.cds1CDS
Lcy02g000320.1.cds2Lcy02g000320.1.cds2CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy02g000320.1.utr3p1Lcy02g000320.1.utr3p1three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lcy02g000320.1Lcy02g000320.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane