Lag0035694.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0035694.1 vs. NCBI nr
Match: XP_023874613.1 (uncharacterized protein LOC111987139 [Quercus suber])

HSP 1 Score: 382.9 bits (982), Expect = 5.7e-102
Identity = 252/662 (38.07%), Postives = 347/662 (52.42%), Query Frame = 0

Query: 1   MNGVLADAIRLRLFPFSLQDKAKDWLESVETGSISTWDELAQAFLTKFFPPAKTTKLRTE 60
           MNGV  D IRLRLFPFSL+DKA+ WL+S++ GSI++W ++A+ FL KFFPPAKT +LR+E
Sbjct: 77  MNGVTEDTIRLRLFPFSLRDKARGWLQSLQPGSITSWQDMAEKFLAKFFPPAKTAQLRSE 136

Query: 61  IGTFRQLNEEQLYEAWEKYKEMLRRCPQSRYPELLQ------------------------ 120
           IG FRQ + E LYEAWE+YK+++R CPQ   P+ LQ                        
Sbjct: 137 IGQFRQNDFESLYEAWERYKDLIRCCPQHGLPDWLQVQMFYNGLNGQTRTIVDAASGGTL 196

Query: 121 -------------------------------KARLYELDESSSLKAQLASLTNALNKLTS 180
                                           A ++EL+  ++L AQ+ASL++ ++ LT+
Sbjct: 197 MSKTAEGATSLLEEMASNNYQWPTERTMAKKVAGIHELEPFAALSAQVASLSHQVSALTT 256

Query: 181 PEVVKSISTLVEGHSKKEGQDV--EEVQYIGNRPY---AQGVPNFYHPSLRNYENLSYSN 240
             + +    +          +   E+VQYI NR Y      +PN+YHP LRN+EN SY N
Sbjct: 257 QRIPQGAEYVAASSMTVPMNEASQEQVQYINNRNYNYRGNPMPNYYHPGLRNHENFSYGN 316

Query: 241 TKNVLQPPPGFASSSAPEKKNNQEEMV-------ALFIKEHRILNVSLQTSVNNHDAALK 300
           TKNVLQPPPGF S  + +K + ++ MV       A F K    L+ +++T  +N  A +K
Sbjct: 317 TKNVLQPPPGFDSQPSEKKMSLEDAMVSFVEETKATFKKSDSQLD-NIETHCSNMGATMK 376

Query: 301 NMEVQIGQIASVVNALQKGKFPSDTEPNPKEQCKMVILRSGRRLEKSSKEEEKRRDEYER 360
           N+EVQIGQ+A+ +NA Q+G FPS+TE NPKEQCK + LRSGR +E+S  +E         
Sbjct: 377 NLEVQIGQLATTINAQQRGTFPSNTEVNPKEQCKAITLRSGREIERSPSKE--------- 436

Query: 361 TEAQKASSERFQRPPNSIELKCDFSNKLADRKEDERQDDKKKLTEEEVV--PCNHHDRGS 420
                  +E     PN+                      K K+ EEE+V       D   
Sbjct: 437 -------TETTPTAPNN-------------------GQSKNKVEEEEIVEDTLRETDMPP 496

Query: 421 HISLPKRRGECPTFDFRELPFPQRFKNVKLDEQFQKFLEMFKKLSVNIPLVEALYNMPNY 480
            IS P    + P      LP+PQRF+  KLD+QF KFL++FKK+ +NIP  +AL  MPNY
Sbjct: 497 SISFP----DNPPILSTPLPYPQRFQKQKLDKQFSKFLDIFKKIHINIPFADALEQMPNY 556

Query: 481 GKFMKEMLSRKKSLKK-EVFNLSESSSTIVS----------GSFTVPCTIGEVSFDRALC 538
            KF+K+++S+K+ L++ E   LSE  S I+           GSFT+PCTIG   FD+ LC
Sbjct: 557 AKFLKDIISKKRRLEEFETVKLSEECSAIIQKKLPQKLKDPGSFTLPCTIGNSFFDKVLC 616

BLAST of Lag0035694.1 vs. NCBI nr
Match: KAG7947748.1 (hypothetical protein I3843_14G109500 [Carya illinoinensis])

HSP 1 Score: 377.1 bits (967), Expect = 3.1e-100
Identity = 258/676 (38.17%), Postives = 355/676 (52.51%), Query Frame = 0

Query: 1   MNGVLADAIRLRLFPFSLQDKAKDWLESVETGSISTWDELAQAFLTKFFPPAKTTKLRTE 60
           +NGV  D IRLRLFPFSL+DKA+ WL+S++ GSI +W ++A+ FL KFFPPAKT +LR+E
Sbjct: 77  INGVTEDTIRLRLFPFSLRDKARGWLQSLQPGSIVSWQDMAERFLAKFFPPAKTAQLRSE 136

Query: 61  IGTFRQLNEEQLYEAWEKYKEMLRRCPQSRYPELLQ------------------------ 120
           IG F+Q + E LYEAWE+YK+++RRCPQ   P+ LQ                        
Sbjct: 137 IGQFKQNDFESLYEAWERYKDLIRRCPQHGLPDWLQVQMFYNGLNGQTRTIVDAASGGTL 196

Query: 121 -------------------------------KARLYELDESSSLKAQLASLTNALNKLT- 180
                                           A ++EL+  ++L AQ+A+L++ ++ LT 
Sbjct: 197 MSKTAEGATALLEEMASNNYQWPTERTLAKKVAGIHELEPIAALSAQVATLSHQISALTT 256

Query: 181 -----SPEVVKSISTLVEGHSKKEGQDVEEVQYIGNRPY---AQGVPNFYHPSLRNYENL 240
                S E V S S +V  +   +    E+VQY+ NR Y      +PN+YHP LRN+ENL
Sbjct: 257 QRIPQSTEYVASTSMIVPSNEASQ----EQVQYVNNRNYNYRGNPMPNYYHPGLRNHENL 316

Query: 241 SYSNTKNVLQP--PPGFASSSAPEKKNNQEEMVALFIKE---------HRILNVSLQTSV 300
           SY NTKNVLQP  PPGF  S   EKK + E+ +  F++E          R+ N+  +T  
Sbjct: 317 SYGNTKNVLQPQHPPGF-DSQPSEKKMSLEDAMVSFVQETNARFKKTDSRLDNI--ETHC 376

Query: 301 NNHDAALKNMEVQIGQIASVVNALQKGKFPSDTEPNPKEQCKMVILRSGRRLEKSSKEEE 360
           +N  A +KN+EVQIGQ+A+ +NA Q+G FPS+TE NPKEQCK + LRSG+ +E++  +E 
Sbjct: 377 SNMGATMKNLEVQIGQLATTINAQQRGAFPSNTEVNPKEQCKAITLRSGKEIERAPLKES 436

Query: 361 KRRDEYERTEAQKASSERFQRPPNSIELKCDFSNKLADRKEDERQDDKKKLTEEEVVPCN 420
           K       T A    S                               K ++ EEE+V   
Sbjct: 437 KSTP----TAANNGQS-------------------------------KDQVEEEEIV--- 496

Query: 421 HHDRGSHISLPKRRGECPTFDFRE--------LPFPQRFKNVKLDEQFQKFLEMFKKLSV 480
            +D      LP      PT  F +        LP+PQRF+  KLD+QF KFL++FKK+ +
Sbjct: 497 -NDTLEETDLP------PTISFPDNPPILAPPLPYPQRFQKQKLDKQFSKFLDIFKKIHI 556

Query: 481 NIPLVEALYNMPNYGKFMKEMLSRKKSLKK-EVFNLSESSSTIVS----------GSFTV 538
           NIP  +AL  MPNY KF+K+++S+K+ L++ E   LSE  S I+           GSFT+
Sbjct: 557 NIPFADALEQMPNYVKFLKDIISKKRRLEEFETVKLSEECSAILQKKLPQKLKDPGSFTL 616

BLAST of Lag0035694.1 vs. NCBI nr
Match: KAG7990634.1 (hypothetical protein I3843_02G035100 [Carya illinoinensis])

HSP 1 Score: 374.8 bits (961), Expect = 1.6e-99
Identity = 253/668 (37.87%), Postives = 351/668 (52.54%), Query Frame = 0

Query: 1   MNGVLADAIRLRLFPFSLQDKAKDWLESVETGSISTWDELAQAFLTKFFPPAKTTKLRTE 60
           +NGV  D IRLRLFPFSL+DKA+ WL+S++ GSI +W ++A+ FL KFFPPAKT +LR+E
Sbjct: 107 INGVTEDTIRLRLFPFSLRDKARGWLQSLQPGSIVSWQDMAERFLAKFFPPAKTAQLRSE 166

Query: 61  IGTFRQLNEEQLYEAWEKYKEMLRRCPQSRYPELLQ------------------------ 120
           IG F+Q + E LYEAWE+YK+++RRCPQ   P+ LQ                        
Sbjct: 167 IGQFKQNDFESLYEAWERYKDLIRRCPQHGLPDWLQVQMFYNGLNGQTRTIVDAASGGTL 226

Query: 121 -------------------------------KARLYELDESSSLKAQLASLTNALNKLT- 180
                                           A +++L+  ++L AQ+A+L++ ++ LT 
Sbjct: 227 MSKTAEGATALLEEMASNNYQWPTERTLAKKVAGIHDLEPIAALSAQVATLSHQISALTT 286

Query: 181 -----SPEVVKSISTLVEGHSKKEGQDVEEVQYIGNRPY---AQGVPNFYHPSLRNYENL 240
                S E + S S +V  +   +    E+VQY+ NR Y      +PN+YHP LRN+ENL
Sbjct: 287 QRIPQSTEYLASTSMIVPSNEASQ----EQVQYVNNRNYNYRGNPMPNYYHPGLRNHENL 346

Query: 241 SYSNTKNVLQP--PPGFASSSAPEKKNNQEEMV-------ALFIKEHRILNVSLQTSVNN 300
           SY NTKNVLQP  PPGF S  +  K + ++ MV       A F K    L+ +++T  +N
Sbjct: 347 SYGNTKNVLQPQHPPGFDSQPSERKMSLEDAMVSFVQETNARFKKTDSRLD-NIETHCSN 406

Query: 301 HDAALKNMEVQIGQIASVVNALQKGKFPSDTEPNPKEQCKMVILRSGRRLEKSSKEEEKR 360
             AA+KN+EVQIGQ+A+ +NA Q+G FPS+TE NPKEQCK + LRSG+ +E+S  +E K 
Sbjct: 407 MGAAIKNIEVQIGQLATTINAQQRGAFPSNTEVNPKEQCKAITLRSGKEIERSPLKESK- 466

Query: 361 RDEYERTEAQKASSERFQRPPNSIELKCDFSNKLADRKEDERQDDKKKLTEEEVV--PCN 420
                               P ++ +                   K K+ E+E+V     
Sbjct: 467 ------------------STPTAVNI----------------GQSKNKVEEDEIVNDTLE 526

Query: 421 HHDRGSHISLPKRRGECPTFDFRELPFPQRFKNVKLDEQFQKFLEMFKKLSVNIPLVEAL 480
             D    IS P    + P      LP+PQRF+  KLD+QF KFL++FKK+ +NIP  +AL
Sbjct: 527 ETDFAPTISFP----DNPPILAPPLPYPQRFQKQKLDKQFSKFLDIFKKIHINIPFADAL 586

Query: 481 YNMPNYGKFMKEMLSRKKSLKK-EVFNLSESSSTIVS----------GSFTVPCTIGEVS 538
             MPNY KF+K+++S+K+ L++ E   LSE  S I+           GSFT+PCTIG+  
Sbjct: 587 EQMPNYVKFLKDIISKKRRLEEFETVKLSEECSAILQKKLPQKLKDPGSFTLPCTIGDSF 646

BLAST of Lag0035694.1 vs. NCBI nr
Match: KAG6734747.1 (hypothetical protein I3842_01G285500 [Carya illinoinensis])

HSP 1 Score: 371.7 bits (953), Expect = 1.3e-98
Identity = 256/676 (37.87%), Postives = 353/676 (52.22%), Query Frame = 0

Query: 1   MNGVLADAIRLRLFPFSLQDKAKDWLESVETGSISTWDELAQAFLTKFFPPAKTTKLRTE 60
           +NGV  D IRLRLFPFSL+DKA+ WL+S++ GSI +W ++A+ FL KFFPPAKT +LR+E
Sbjct: 77  INGVTEDTIRLRLFPFSLRDKARGWLQSLQPGSIVSWQDMAERFLAKFFPPAKTAQLRSE 136

Query: 61  IGTFRQLNEEQLYEAWEKYKEMLRRCPQSRYPELLQ------------------------ 120
           IG F+Q + E LYEAWE+YK+++RRCPQ   P+ LQ                        
Sbjct: 137 IGQFKQNDFESLYEAWERYKDLIRRCPQHGLPDWLQVQMFYNGLNGQTRTIVDAASGGTL 196

Query: 121 -------------------------------KARLYELDESSSLKAQLASLTNALNKLT- 180
                                           A ++EL+  ++L AQ+A+L++ ++ LT 
Sbjct: 197 MSKTAEGATALLEEMASNNYQWPTERTLAKKVAGIHELEPIAALSAQVATLSHQISALTT 256

Query: 181 -----SPEVVKSISTLVEGHSKKEGQDVEEVQYIGNRPY---AQGVPNFYHPSLRNYENL 240
                S E V S S +V  +   +    E+VQY+ NR Y      +PN+YHP LRN+ENL
Sbjct: 257 QRIPQSTEYVASTSMIVPSNEASQ----EQVQYVNNRNYNYRGNPMPNYYHPGLRNHENL 316

Query: 241 SYSNTKNVLQP--PPGFASSSAPEKKNNQEEMVALFIKE---------HRILNVSLQTSV 300
           SY NTKNVLQP  PPGF  S   EKK + E+ +  F++E          R+ N+  +T  
Sbjct: 317 SYGNTKNVLQPQHPPGF-DSQPSEKKMSLEDAMVSFVQETNARFKKTDSRLDNI--ETHC 376

Query: 301 NNHDAALKNMEVQIGQIASVVNALQKGKFPSDTEPNPKEQCKMVILRSGRRLEKSSKEEE 360
           +N  A +KN+EVQIGQ+A+ +NA Q+G FPS+TE NPKEQCK + LRSG+ +E++  +E 
Sbjct: 377 SNMGATMKNLEVQIGQLATTINAQQRGAFPSNTEVNPKEQCKAITLRSGKEIERAPLKES 436

Query: 361 KRRDEYERTEAQKASSERFQRPPNSIELKCDFSNKLADRKEDERQDDKKKLTEEEVVPCN 420
           K       T A    S                               K ++ EEE+V   
Sbjct: 437 KSTP----TAANNGQS-------------------------------KDQVEEEEIV--- 496

Query: 421 HHDRGSHISLPKRRGECPTFDFRE--------LPFPQRFKNVKLDEQFQKFLEMFKKLSV 480
            +D      LP      PT  F +        LP+PQRF+  KLD+QF KFL++FKK+ +
Sbjct: 497 -NDTLEETDLP------PTISFPDNPPILAPPLPYPQRFQKQKLDKQFSKFLDIFKKIHI 556

Query: 481 NIPLVEALYNMPNYGKFMKEMLSRKKSLKK-EVFNLSESSSTIVS----------GSFTV 538
           NIP  +AL  MPNY KF+K+++S+K+ L++ E   LSE  S I+            SFT+
Sbjct: 557 NIPFADALEQMPNYVKFLKDIISKKRRLEEFETVKLSEECSAILQKKLPQKLKDPESFTL 616

BLAST of Lag0035694.1 vs. NCBI nr
Match: XP_022860306.1 (uncharacterized protein LOC111380876 [Olea europaea var. sylvestris])

HSP 1 Score: 357.5 bits (916), Expect = 2.6e-94
Identity = 243/658 (36.93%), Postives = 344/658 (52.28%), Query Frame = 0

Query: 1   MNGVLADAIRLRLFPFSLQDKAKDWLESVETGSISTWDELAQAFLTKFFPPAKTTKLRTE 60
           MNGV  DAIRLRLF FSL+DKAK W +S+  GSI+TWD+LAQ FLTK+FPP+K+T+L +E
Sbjct: 82  MNGVTEDAIRLRLFSFSLRDKAKAWFQSLPYGSITTWDDLAQKFLTKYFPPSKSTQLHSE 141

Query: 61  IGTFRQLNEEQLYEAWEKYKEMLRRCPQSRYPELLQ------------------------ 120
           I  F+QL+ E  YEAWE++K++LRRCPQ  + + +Q                        
Sbjct: 142 ISQFKQLDFEPFYEAWERFKDLLRRCPQHGFQKWMQIEIFYNGLNGQTRTMVDAAAGGIL 201

Query: 121 -------------------------------KARLYELDESSSLKAQLASLTNALNKLTS 180
                                           A  +E+D  ++L AQ+ASLTN +  LT+
Sbjct: 202 MAKTAEAAYALLDDIATNSYQWPSERSGVKKVAGFHEVDPITALAAQVASLTNQIVTLTT 261

Query: 181 PEVVKSISTLVEGHSKKEGQDV--EEVQYIGNRPYAQG---VPNFYHPSLRNYENLSYSN 240
               + + +++   S  +  +V  E+ QY+ +R Y Q      N YHP LRN++NLSY N
Sbjct: 262 QGNQQKVDSIMSASSSNQETEVTNEQAQYVDSRNYNQRGSYQANHYHPGLRNHKNLSYGN 321

Query: 241 TKNVLQPPPGFASSSAPEKKNNQEEMVALFIKEHR-------ILNVSLQTSVNNHDAALK 300
            +N LQPPP F + ++ + K   E+++  FI E R       +   +++T V+N  A +K
Sbjct: 322 NRNTLQPPPEFNTQNS-DGKPPLEDILGTFISETRSRFNKNELRWDNIETHVSNMGATMK 381

Query: 301 NMEVQIGQIASVVNALQKGKFPSDTEPNPKEQCKMVILRSGRRLEKSSKEEEKRRDEYER 360
           N+EVQIGQ+A+ + + QKGKFP DTE NP+E C  +ILRSG+ +E+S             
Sbjct: 382 NLEVQIGQLATSMKSQQKGKFPIDTEVNPREHCNAIILRSGKMVEES------------- 441

Query: 361 TEAQKASSERFQRPPNSIELKCDFSNKLADRKEDERQDDKKKLTEEEVVPCNHHDRGSHI 420
                   ++   P + + +             +ERQ +++K TE E         G+ I
Sbjct: 442 ------KPKKIVVPTSDVIV------------TEERQSERQK-TEAE---------GTKI 501

Query: 421 SLPKRRG--ECPTFDFRELPFPQRFKNVKLDEQFQKFLEMFKKLSVNIPLVEALYNMPNY 480
             P      + P      LPFPQRF   K D+QF KFLE+FKK+ +NIP  EAL  MPNY
Sbjct: 502 YKPYSLSFPDNPPILKPPLPFPQRFMKKKFDDQFAKFLEVFKKIHINIPFAEALAQMPNY 561

Query: 481 GKFMKEMLSRKKSLKK-EVFNLSESSSTIV---------SGSFTVPCTIGEVSFDRALCD 535
            KF+KE++S KK L++ E   L+E  S I+         SGSF +PC IG ++FDRALCD
Sbjct: 562 AKFLKEVMSNKKKLEEFETIKLTEGCSDILQKLPHKLKDSGSFNIPCNIGGITFDRALCD 621

BLAST of Lag0035694.1 vs. ExPASy TrEMBL
Match: A0A6J0ZX64 (LOW QUALITY PROTEIN: uncharacterized protein LOC110412945 OS=Herrania umbratica OX=108875 GN=LOC110412945 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 5.8e-76
Identity = 223/659 (33.84%), Postives = 316/659 (47.95%), Query Frame = 0

Query: 2   NGVLADAIRLRLFPFSLQDKAKDWLESVETGSISTWDELAQAFLTKFFPPAKTTKLRTEI 61
           NGV  DAIRLRLFPFSL+DKAK WL S+  GSI+TW++LAQ FL KFFPPAKT K+R +I
Sbjct: 125 NGVTDDAIRLRLFPFSLRDKAKSWLNSLPNGSITTWEDLAQKFLAKFFPPAKTAKMRNDI 184

Query: 62  GTFRQLNEEQLYEAWEKYKEMLRRCPQSRYPELLQ------------------------- 121
            +F Q + E LYEAWE++KE+LRRCP    P+ LQ                         
Sbjct: 185 TSFIQFDGESLYEAWERFKELLRRCPHHGIPDWLQVQTFYNGLVGSIKTIIDAAAGGALM 244

Query: 122 ------------------------------KARLYELDESSSLKAQLASLTNALNKLTSP 181
                                             YE+D   +L  Q+A+L+  L+ L   
Sbjct: 245 SKNAVDAYNLLEEMASNNYQWPSERSGSRKAVGAYEIDALGTLTTQVAALSKKLDTLGVH 304

Query: 182 EVVKSI---STLVEGHSKKE-GQDVEEVQYIGNRPYAQGVP--NFYHPSLRNYENLSYSN 241
            V  S+       + HS  +   + E VQ++GN    Q  P  N Y+P  RN+ N S+SN
Sbjct: 305 AVQNSLVVCEMCGDSHSYDQCPYNSESVQFVGNFNRQQNNPYSNTYNPGWRNHPNFSWSN 364

Query: 242 TKNVLQP----PPGF---ASSSAPEKKNNQEEMVALFIKEHRILNVSLQTSVNNHDAALK 301
                 P    PPGF   A    PEKK+  EE++  +I +           + +  A+L+
Sbjct: 365 NAGPSNPKPIMPPGFQQQARPQIPEKKSQLEELLLQYISK-------TDAIIQSQGASLR 424

Query: 302 NMEVQIGQIASVVNALQKGKFPSDTEPNP--KEQCKMVILRSGRRLEKSSKEEEKRRDEY 361
           N+E Q+GQ+A+ +N   +G  PSDT+ NP  KEQC+ + LRSG+ +E  +++  +   E+
Sbjct: 425 NLETQVGQLANSINNRPQGSLPSDTQINPKGKEQCQAITLRSGKEIEGVNQKAVESEIEH 484

Query: 362 ERTEAQKASSERFQRPPNSIELKCDFSNKLADRKEDERQDDKKKLTEEEVVPCNHHDRGS 421
              E             N IE++         +K+D++ +++   T + + P        
Sbjct: 485 VDKEGM---------CENEIEIQ---------QKDDDKAENQG--TSQVIHP-------- 544

Query: 422 HISLPKRRGECPTFDFRELPFPQRFKNVKLDEQFQKFLEMFKKLSVNIPLVEALYNMPNY 481
                              PFPQR +  KL++QFQKFL +FKKL +NIP  EAL  MP+Y
Sbjct: 545 -----------------PPPFPQRLQKQKLEKQFQKFLNVFKKLHINIPFAEALEQMPSY 604

Query: 482 GKFMKEMLSRKKSLKK-EVFNLSESSSTIVS----------GSFTVPCTIGEVSFDRALC 535
            KF+K++LS+K+ L + E   L+E  S I+           GSFT+PCTIG + F +AL 
Sbjct: 605 VKFLKDILSKKRKLGEFETVFLTEECSAILQNKLPPKLKDPGSFTIPCTIGNLFFTKALS 664

BLAST of Lag0035694.1 vs. ExPASy TrEMBL
Match: A0A6P6TF62 (Reverse transcriptase OS=Coffea arabica OX=13443 GN=LOC113700883 PE=4 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 6.2e-70
Identity = 216/663 (32.58%), Postives = 319/663 (48.11%), Query Frame = 0

Query: 1   MNGVLADAIRLRLFPFSLQDKAKDWLESVETGSISTWDELAQAFLTKFFPPAKTTKLRTE 60
           MNGV  +AIRLRLFPFSL+DKAK WL S    + +TWD+L++AFL K+FPP KT KLR +
Sbjct: 1   MNGVSDEAIRLRLFPFSLRDKAKLWLHSHAPNTFTTWDDLSRAFLNKYFPPGKTAKLRMD 60

Query: 61  IGTFRQLNEEQLYEAWEKYKEMLRRCPQSRYPELLQKARLY------------------- 120
           I  F Q+  E LYEAWE+++++LR+CP    PE L     Y                   
Sbjct: 61  ITGFSQMEGESLYEAWERFRDLLRKCPHHGLPEWLIIQTFYNGLSFSTKTMIDAAAGGAL 120

Query: 121 ----ELDESSSLKAQLASLTNALNK------LTSPEVVKSISTLVEGHSKKEGQDVEEVQ 180
               E+D  + L AQ+ ++   L++        S   +   S     H   E  D E+VQ
Sbjct: 121 MGMIEMDTLNMLSAQMNNVMKLLSRQGGVGPSASNAHIACCSLCGGEHDTNECVDSEQVQ 180

Query: 181 YIGN-RPYAQGVP--NFYHPSLRNYENLSYSNTKNVLQP--PPGFAS-SSAPEKKNNQEE 240
           ++ N    AQ  P  N Y+P  RN+ N  + +  N  +P  PPGF S     E K   E 
Sbjct: 181 FVNNYNRNAQNNPYSNTYNPGWRNHPNFGWKDQGNQSRPANPPGFQSRQPQAETKPGWEI 240

Query: 241 MVALFIKEHRILNVSLQTSVNNHDAALKNMEVQIGQIASVVNALQKGKFPSDTEPNPKEQ 300
            V    K        ++  ++      +N+EVQIGQIAS +N   +G+ PS TE NPKE 
Sbjct: 241 AVEKLAKVTSDRFERVEGRLDQLTTMYRNVEVQIGQIASSLNNRNQGELPSKTEVNPKEH 300

Query: 301 CKMVILRSGRRLEKSSKEEEKRRDEYERTEAQKASSERFQRPPNSIELKCDFSNKLADRK 360
            K + LRSG++LE       K+ +  E  E Q+                    N+ A  +
Sbjct: 301 VKAITLRSGKQLEDPPVSGGKKDESEENAEKQR--------------------NQEATME 360

Query: 361 EDERQDDKKKLTEEEVVPCNHHDRGSHISLPKRRGECPTFDFRELPFPQRFKNVKLDEQF 420
            + RQ+ +      E  P +     ++I +P             +PFPQR K  K D+ F
Sbjct: 361 GNSRQNPR------ETQPSS----STNIPIPP-----------AVPFPQRLKQNKFDKDF 420

Query: 421 QKFLEMFKKLSVNIPLVEALYNMPNYGKFMKEMLSRKKSLKK-EVFNLSESSSTIVS--- 480
           +KF++MFK+L +NIP  +A+  +P+Y KF+KE+++RK+ L+  E   L+E  S I+    
Sbjct: 421 EKFVKMFKQLHINIPFADAILQIPSYAKFLKEIMTRKRKLEDCETIALTEECSAIIQNKL 480

Query: 481 -------GSFTVPCTIGEVSFDRALCDLGASINLMSYSVFRKIGLSGGQ----------- 540
                  GSF++PCTIG ++F +ALCDLGAS++L+  +V R++GL   +           
Sbjct: 481 PPKLKDPGSFSIPCTIGSINFSKALCDLGASVSLIPLTVARQLGLHELKRTNITLQLADR 540

Query: 541 --------------------IGADY--------------LGRPFLATGKAEISVHTGKLT 572
                               I  D+              LGRPFLAT    I V  GKL 
Sbjct: 541 SIRHPLGVLENVLIKVQKFIIPVDFVVLDMEEDVSMPIILGRPFLATAGTIIDVKNGKLK 600

BLAST of Lag0035694.1 vs. ExPASy TrEMBL
Match: A0A6P6X9H2 (Reverse transcriptase OS=Coffea arabica OX=13443 GN=LOC113741122 PE=4 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 1.8e-69
Identity = 215/663 (32.43%), Postives = 318/663 (47.96%), Query Frame = 0

Query: 1   MNGVLADAIRLRLFPFSLQDKAKDWLESVETGSISTWDELAQAFLTKFFPPAKTTKLRTE 60
           MNGV  +AIRLRLFPFSL+DKAK WL S    + +TWD+L++AFL K+FPP KT KLR +
Sbjct: 73  MNGVSDEAIRLRLFPFSLRDKAKLWLHSHAPNTFTTWDDLSRAFLNKYFPPGKTAKLRMD 132

Query: 61  IGTFRQLNEEQLYEAWEKYKEMLRRCPQSRYPELLQKARLY------------------- 120
           I  F Q+  E LYE WE+++++LR+CP    PE L     Y                   
Sbjct: 133 ITGFSQMEGESLYETWERFRDLLRKCPHHGLPEWLIIQTFYNGLSFSTKTMIDAAAGGAL 192

Query: 121 ----ELDESSSLKAQLASLTNALNK------LTSPEVVKSISTLVEGHSKKEGQDVEEVQ 180
               E+D  + L AQ+ ++   L++        S   +   S     H   E  D E+VQ
Sbjct: 193 MGMIEMDTLNMLSAQMNNVMKLLSRQGGVGPSASNAHIACCSLCGGEHDTNECVDSEQVQ 252

Query: 181 YIGN-RPYAQGVP--NFYHPSLRNYENLSYSNTKNVLQP--PPGFAS-SSAPEKKNNQEE 240
           ++ N    AQ  P  N Y+P  RN+ N  + +  N  +P  PPGF S     E K   E 
Sbjct: 253 FVNNYNRNAQNNPYSNTYNPGWRNHPNFGWKDQGNQSRPANPPGFQSRQPQAETKPGWEI 312

Query: 241 MVALFIKEHRILNVSLQTSVNNHDAALKNMEVQIGQIASVVNALQKGKFPSDTEPNPKEQ 300
            V    K        ++  ++      +N+EVQIGQIAS +N   +G+ PS TE NPKE 
Sbjct: 313 AVEKLAKVTSDRFERVEGRLDQLTTMYRNVEVQIGQIASSLNNRNQGELPSKTEVNPKEH 372

Query: 301 CKMVILRSGRRLEKSSKEEEKRRDEYERTEAQKASSERFQRPPNSIELKCDFSNKLADRK 360
            K + LRSG++LE       K+ +  E  E Q+                    N+ A  +
Sbjct: 373 VKAITLRSGKQLEDPPVSGGKKDESEENAEKQR--------------------NQEATME 432

Query: 361 EDERQDDKKKLTEEEVVPCNHHDRGSHISLPKRRGECPTFDFRELPFPQRFKNVKLDEQF 420
            + RQ+ +      E  P +     ++I +P             +PFPQR K  K D+ F
Sbjct: 433 GNSRQNPR------ETQPSS----STNIPIPP-----------AVPFPQRLKQNKFDKDF 492

Query: 421 QKFLEMFKKLSVNIPLVEALYNMPNYGKFMKEMLSRKKSLKK-EVFNLSESSSTIVS--- 480
           +KF++MFK+L +NIP  +A+  +P+Y KF+KE+++RK+ L+  E   L+E  S I+    
Sbjct: 493 EKFVKMFKQLHINIPFADAILQIPSYAKFLKEIMTRKRKLEDCETIALTEECSAIIQNKL 552

Query: 481 -------GSFTVPCTIGEVSFDRALCDLGASINLMSYSVFRKIGLSGGQ----------- 540
                  GSF++PCTIG ++F +ALCDLGAS++L+  +V R++GL   +           
Sbjct: 553 PPKLKDPGSFSIPCTIGSINFSKALCDLGASVSLIPLTVARQLGLHELKRTNITLQLADR 612

Query: 541 --------------------IGADY--------------LGRPFLATGKAEISVHTGKLT 572
                               I  D+              LGRPFLAT    I V  GKL 
Sbjct: 613 SIRHPLGVLENVLIKVQKFIIPVDFVVLDMEEDVSMPIILGRPFLATAGTIIDVKNGKLK 672

BLAST of Lag0035694.1 vs. ExPASy TrEMBL
Match: A0A6P8DKJ2 (uncharacterized protein LOC116204231 OS=Punica granatum OX=22663 GN=LOC116204231 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 1.9e-66
Identity = 207/685 (30.22%), Postives = 304/685 (44.38%), Query Frame = 0

Query: 1   MNGVLADAIRLRLFPFSLQDKAKDWLESVETGSISTWDELAQAFLTKFFPPAKTTKLRTE 60
           MN V  D IRL+LFPFSL+DKA+ W  S+   SI+TW +L+  FL +FFPPA+T +LR E
Sbjct: 222 MNNVTDDVIRLQLFPFSLRDKARAWFNSLPQESITTWADLSSKFLRRFFPPARTARLRNE 281

Query: 61  IGTFRQLNEEQLYEAWEKYKEMLRRCPQSRYPELL------------------------- 120
           I  F + N E LYEAWE++KE +R+CP    P+ L                         
Sbjct: 282 ITNFTKFNGESLYEAWERFKEAIRKCPHHGLPDNLLIEVFYLSLDDTLRSLVDAAAGGAL 341

Query: 121 -----------------------------QKARLYELDESSSLKAQLASLTNALNKLTSP 180
                                        + A + ++D  ++L  Q+++LT  ++KLTS 
Sbjct: 342 MGKNYDEASALIEEMASSAHNWQNERSKSRVASVNDMDTIANLTTQISALTTQVSKLTSA 401

Query: 181 EVVK----SISTLVEG-HSKKE--------GQDVEEVQYIGNRPYAQGVP--NFYHPSLR 240
                   +   L  G HS  E          + E+V ++ N   +   P  N Y+P  R
Sbjct: 402 HSFNTNQVAFCELCSGPHSTLECMSGNPSASPNGEQVNFVNNFQRSNQGPYSNTYNPGWR 461

Query: 241 NYENLSYSNTKNVLQPPPGF-----ASSSAPEKKNNQEEMVALFIKEHRILNVSLQTSVN 300
           N+ N S+ N  N L+PPPGF     A ++ P++  ++ E + L   +         T + 
Sbjct: 462 NHPNFSWRNENNALKPPPGFQKQGPAQNAPPQQSQSRMEELMLSYMQ------KTDTMLQ 521

Query: 301 NHDAALKNMEVQIGQIASVVNALQKGKFPSDTEPNPKEQCKMVILRSGRRLEKSSKEEEK 360
           N  A ++N+E QI QI+  ++    G  PS+TE NPK     ++LRSG+ LE        
Sbjct: 522 NQQATIRNLEGQISQISQQLSNRPSGSLPSNTEENPK-GVNAIMLRSGKELE-------- 581

Query: 361 RRDEYERTEAQKASSERFQRPPNSIELKCDFSNKLADRKEDERQDDKKKLTEEEVVPCNH 420
                                           N+ A  +E+  + DK K   EE      
Sbjct: 582 ------------------------------IVNRKAQTQEESPEKDKGKQKVEE------ 641

Query: 421 HDRGSHISLPKRRGECPTFDFRELPFPQRFKNVKLDEQFQKFLEMFKKLSVNIPLVEALY 480
                    P+R+          +PFP R K  +LD QF KFL++FKKL +NIP  EAL 
Sbjct: 642 ---------PRRKSLGVKPYVPPVPFPGRLKQQQLDAQFAKFLDVFKKLQINIPFAEALQ 701

Query: 481 NMPNYGKFMKEMLSRKKS----------------LKKEVFNLSESSSTIVSGSFTVPCTI 540
            MP+Y +FMK++L++K+                 L+K++ NL         GSFTVPCTI
Sbjct: 702 QMPSYARFMKDLLTKKRKFDGSEPVMLTGECSMILQKDLPNLPRKQRD--QGSFTVPCTI 761

Query: 541 GEVSFDRALCDLGASINLMSYSVFRKIGLSGGQ--------------------------- 549
           G   F+  L D GASINLM  S+FRK+GL   +                           
Sbjct: 762 GNFHFENVLIDSGASINLMPLSIFRKLGLGECKKTHITLQLADRSIKYPKGIVENVLVKV 821

BLAST of Lag0035694.1 vs. ExPASy TrEMBL
Match: A0A6P8DD93 (uncharacterized protein LOC116206453 OS=Punica granatum OX=22663 GN=LOC116206453 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 4.6e-65
Identity = 205/685 (29.93%), Postives = 310/685 (45.26%), Query Frame = 0

Query: 1   MNGVLADAIRLRLFPFSLQDKAKDWLESVETGSISTWDELAQAFLTKFFPPAKTTKLRTE 60
           MN V  D IRL+LFPFSL+DKA+ W  S+   SI+TW +L+  FL +FFPPA+T +LR E
Sbjct: 116 MNNVTDDVIRLQLFPFSLRDKARAWFNSLPQESITTWADLSSKFLRRFFPPARTARLRNE 175

Query: 61  IGTFRQLNEEQLYEAWEKYKEMLRRCPQSRYPELL------------------------- 120
           I  F + N E LYEAWE++KE +R+CP    P+ L                         
Sbjct: 176 ITNFTKFNGESLYEAWERFKEAIRKCPHHGLPDNLLIEVFYLSLDDTLRSLVDAAAGGAL 235

Query: 121 -----------------------------QKARLYELDESSSLKAQLASLTNALNKLTSP 180
                                        + A + ++D  ++L  Q+++LT  ++KLTS 
Sbjct: 236 MGKNYDEASALIEEMASSAHNWQNERSKSRVASVNDMDTIANLTTQISALTTQVSKLTSA 295

Query: 181 EVVK----SISTLVEG-HSKKE--------GQDVEEVQYIGNRPYAQGVP--NFYHPSLR 240
                   +   L  G HS  E          + E+V ++ N   +   P  N Y+P  R
Sbjct: 296 HSFNTNQVAFCELCSGPHSTLECMSGNPSASPNGEQVNFVNNFQRSNQGPYSNTYNPGWR 355

Query: 241 NYENLSYSNTKNVLQPPPGF-----ASSSAPEKKNNQEEMVALFIKEHRILNVSLQTSVN 300
           N+ N S+ N  N L+PPPGF     A ++ P++  ++ E + L   +         T + 
Sbjct: 356 NHPNFSWRNENNALKPPPGFQKQGPAQNAPPQQSQSRMEELMLSYMQ------KTDTMLQ 415

Query: 301 NHDAALKNMEVQIGQIASVVNALQKGKFPSDTEPNPKEQCKMVILRSGRRLEKSSKEEEK 360
           N  A ++N+E QI QI+  ++    G  PS+TE NPK     ++LRSG+ LE  +++ + 
Sbjct: 416 NQQATIRNLEGQISQISQQLSNRPSGSLPSNTEENPK-GVNAIMLRSGKELEIVNRKAQT 475

Query: 361 RRDEYERTEAQKASSERFQRPPNSIELKCDFSNKLADRKEDERQDDKKKLTEEEVVPCNH 420
           + +  E+ + ++                         + E+ RQ   K L  +  VP   
Sbjct: 476 QEESPEKDKGKQ-------------------------KVEEPRQ---KSLGVKPYVP--- 535

Query: 421 HDRGSHISLPKRRGECPTFDFRELPFPQRFKNVKLDEQFQKFLEMFKKLSVNIPLVEALY 480
                                  +PFP+R K  +LD QF KFL++FKKL +NIP  EAL 
Sbjct: 536 ----------------------PVPFPRRLKQQQLDAQFAKFLDVFKKLQINIPFAEALQ 595

Query: 481 NMPNYGKFMKEMLSRKKS----------------LKKEVFNLSESSSTIVSGSFTVPCTI 540
            MP+Y +FMK++L++K+                 L+K++ NL         GSFTVPCTI
Sbjct: 596 QMPSYARFMKDLLTKKRKFDGSEPVMLTGECSMILQKDLPNLPRKQRD--QGSFTVPCTI 655

Query: 541 GEVSFDRALCDLGASINLMSYSVFRKIGLSGGQ--------------------------- 549
           G   F+  L D GASINLM  S+FRK+GL   +                           
Sbjct: 656 GNFHFENVLIDSGASINLMPLSIFRKLGLGECKKTHVTLQLADRSIKYPKGIVENVLVKV 715