Lag0024094.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0024094.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cystatin domain-containing protein
Location	chr10: 483398 .. 483935 (+)
Sequence length	372
RNA-Seq Expression	Lag0024094.1
Synteny	Lag0024094.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTATCCACCCGAATACGTTTGTCCCGAGTCATGTGATGGATGTCATGAACATGACATCCGTGAGATGAATGATGAAGAGCTGGATCTATACTATGAAGCTGTGCATAAAAGCGAGGTTTTCTTTTCAATCCTTTGCTGCAATATTCTTGATTCTCATCTCAAACTCATTACAATATAATTCAAAATTTTCATTTACGACAGGGTTTTGATGTCCCGCATTTTCCTGATTCCTATGCCATTGCTCTGATTGTGCCTATGTCATTTCTAGAAGAGGTTGAAGAATGTGCACAGGAAGCTATTAAACATTACAACAACCAAAATGTATGTATATATGTTTTCATTTTCTGTTTAACTTGTTAGAGGGATCATATATATGAATGTTTTTTTTTCCATATATACAGGGTACAAATTTTGAGGTTGTGAATATTGTGAAGGCGAACAATCAAGCTGCCCGTGGTATCTTATATTTCATCACCTTCGAGGTCAAACCAATTGGAACACCTATGAGAATTGAGAATGTCATCATAGCATAG

mRNA sequence

ATGGCTTATCCACCCGAATACGTTTGTCCCGAGTCATGTGATGGATGTCATGAACATGACATCCGTGAGATGAATGATGAAGAGCTGGATCTATACTATGAAGCTGTGCATAAAAGCGAGGGTTTTGATGTCCCGCATTTTCCTGATTCCTATGCCATTGCTCTGATTGTGCCTATGTCATTTCTAGAAGAGGTTGAAGAATGTGCACAGGAAGCTATTAAACATTACAACAACCAAAATGGTACAAATTTTGAGGTTGTGAATATTGTGAAGGCGAACAATCAAGCTGCCCGTGGTATCTTATATTTCATCACCTTCGAGGTCAAACCAATTGGAACACCTATGAGAATTGAGAATGTCATCATAGCATAG

Coding sequence (CDS)

ATGGCTTATCCACCCGAATACGTTTGTCCCGAGTCATGTGATGGATGTCATGAACATGACATCCGTGAGATGAATGATGAAGAGCTGGATCTATACTATGAAGCTGTGCATAAAAGCGAGGGTTTTGATGTCCCGCATTTTCCTGATTCCTATGCCATTGCTCTGATTGTGCCTATGTCATTTCTAGAAGAGGTTGAAGAATGTGCACAGGAAGCTATTAAACATTACAACAACCAAAATGGTACAAATTTTGAGGTTGTGAATATTGTGAAGGCGAACAATCAAGCTGCCCGTGGTATCTTATATTTCATCACCTTCGAGGTCAAACCAATTGGAACACCTATGAGAATTGAGAATGTCATCATAGCATAG

Protein sequence

MAYPPEYVCPESCDGCHEHDIREMNDEELDLYYEAVHKSEGFDVPHFPDSYAIALIVPMSFLEEVEECAQEAIKHYNNQNGTNFEVVNIVKANNQAARGILYFITFEVKPIGTPMRIENVIIA

Homology

[Close]

BLAST of Lag0024094.1 vs. NCBI nr
Match: XP_022137488.1 (uncharacterized protein LOC111008920 isoform X1 [Momordica charantia])

HSP 1 Score: 124.8 bits (312), Expect = 5.2e-25
Identity = 58/105 (55.24%), Postives = 80/105 (76.19%), Query Frame = 0


Query: 14  DGCHEHDIREMNDEELDLYYEAVHKSEGFDVPHFPDSYAIALIVPMSFL----EEVEECA 73
           DG ++  +REMN+EE++ YY+A+ +SEGFDVP FP +YA A+I P+  +    EEV ECA
Sbjct: 46  DGYYDDGVREMNEEEVNRYYKAIQESEGFDVPSFPGTYAFAIISPLPLINLISEEVRECA 105

Query: 74  QEAIKHYNNQNGTNFEVVNIVKANNQAARGILYFITFEVKPIGTP 115
            +AIKHYNN+NG +FE V ++KAN+QAA G L+F+TF+VK  G P
Sbjct: 106 GQAIKHYNNENGASFEFVKMLKANSQAALGELFFLTFQVKQTGAP 150

[Close]

BLAST of Lag0024094.1 vs. NCBI nr
Match: XP_023525925.1 (uncharacterized protein LOC111789396 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 111.7 bits (278), Expect = 4.6e-21
Identity = 54/107 (50.47%), Postives = 75/107 (70.09%), Query Frame = 0


Query: 11  ESCDGCHEHDIREMNDEELDLYYEAVHKSEGFDVPHFPDSYAIALIVPM--SFLEE--VE 70
           +  D  +EH +R MN EE+  YY+ +  ++GFDVP FP++YA+ LIVP+   +L +  + 
Sbjct: 47  DDSDEEYEHGVRVMNREEMSRYYQVLRDTDGFDVPTFPNTYALGLIVPICEEYLSKPYLR 106

Query: 71  ECAQEAIKHYNNQNGTNFEVVNIVKANNQAARGILYFITFEVKPIGT 114
           +CA++AI HYN +NGTNFE V IVKAN Q   G  Y+ITF+VK IGT
Sbjct: 107 DCAEKAISHYNIRNGTNFEFVKIVKANQQVVSGFFYYITFDVKQIGT 153

[Close]

BLAST of Lag0024094.1 vs. NCBI nr
Match: XP_022942206.1 (uncharacterized protein LOC111447330 [Cucurbita moschata] >XP_022942207.1 uncharacterized protein LOC111447330 [Cucurbita moschata])

HSP 1 Score: 109.4 bits (272), Expect = 2.3e-20
Identity = 56/100 (56.00%), Postives = 72/100 (72.00%), Query Frame = 0


Query: 27  EELDLYYEAVHKSEGFDVPHFPDSYAIALIVPM------SFLEEVEECAQEAIKHYNNQN 86
           EE++ YY AV +S+GFDVP F + YA +LI PM        L+EV+  A EAIKHYNN+N
Sbjct: 33  EEME-YYCAVEESQGFDVPDFGNVYAFSLITPMKLHEVPGMLKEVQISANEAIKHYNNEN 92

Query: 87  GTNFEVVNIVKANNQAARGILYFITFEVKPIGTPMRIENV 121
           GTNFEVV+IVKAN+    G +Y+ITF VKPIGTP+   ++
Sbjct: 93  GTNFEVVDIVKANHSGCCGSMYYITFNVKPIGTPLEFPSI 131

[Close]

BLAST of Lag0024094.1 vs. NCBI nr
Match: XP_023548353.1 (UPF0725 protein At1g02770-like [Cucurbita pepo subsp. pepo] >XP_023548361.1 UPF0725 protein At1g02770-like [Cucurbita pepo subsp. pepo] >XP_023548367.1 UPF0725 protein At1g02770-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 102.1 bits (253), Expect = 3.6e-18
Identity = 54/93 (58.06%), Postives = 67/93 (72.04%), Query Frame = 0


Query: 27  EELDLYYEAVHKSEGFDVPHFPDSYAIALIVPM------SFLEEVEECAQEAIKHYNNQN 86
           EE++ YY AV +S+GFDVP F   YA +LI PM      S L+EV+    EAIKHYNN+N
Sbjct: 33  EEME-YYCAVQESQGFDVPDFGTVYAFSLITPMKLHEFPSMLKEVQISPNEAIKHYNNEN 92

Query: 87  GTNFEVVNIVKANNQAARGILYFITFEVKPIGT 114
           GTNFE+V+IVKAN+    G +Y+ITF VKPIGT
Sbjct: 93  GTNFELVDIVKANHSGCCGSMYYITFNVKPIGT 124

[Close]

BLAST of Lag0024094.1 vs. NCBI nr
Match: XP_023521680.1 (UPF0725 protein At1g02770-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 102.1 bits (253), Expect = 3.6e-18
Identity = 54/93 (58.06%), Postives = 67/93 (72.04%), Query Frame = 0


Query: 27  EELDLYYEAVHKSEGFDVPHFPDSYAIALIVPM------SFLEEVEECAQEAIKHYNNQN 86
           EE++ YY AV +S+GFDVP F   YA +LI PM      S L+EV+    EAIKHYNN+N
Sbjct: 33  EEME-YYCAVQESQGFDVPDFGTVYAFSLITPMKLHEFPSMLKEVQISPNEAIKHYNNEN 92

Query: 87  GTNFEVVNIVKANNQAARGILYFITFEVKPIGT 114
           GTNFE+V+IVKAN+    G +Y+ITF VKPIGT
Sbjct: 93  GTNFELVDIVKANHSGCCGSMYYITFNVKPIGT 124

The following BLAST results are available for this feature:

BLAST of Lag0024094.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137488.1	5.2e-25	55.24	uncharacterized protein LOC111008920 isoform X1 [Momordica charantia]	[more]
XP_023525925.1	4.6e-21	50.47	uncharacterized protein LOC111789396 [Cucurbita pepo subsp. pepo]	[more]
XP_022942206.1	2.3e-20	56.00	uncharacterized protein LOC111447330 [Cucurbita moschata] >XP_022942207.1 unchar...	[more]
XP_023548353.1	3.6e-18	58.06	UPF0725 protein At1g02770-like [Cucurbita pepo subsp. pepo] >XP_023548361.1 UPF0...	[more]
XP_023521680.1	3.6e-18	58.06	UPF0725 protein At1g02770-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0024094.1 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 59..79
None	No IPR available	GENE3D	3.10.450.10		coord: 58..122 e-value: 7.0E-12 score: 47.5
None	No IPR available	PANTHER	PTHR31260	CYSTATIN/MONELLIN SUPERFAMILY PROTEIN	coord: 4..107
None	No IPR available	PANTHER	PTHR31260:SF28	CYSTATIN/MONELLIN SUPERFAMILY PROTEIN	coord: 4..107
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 63..109
IPR000010	Cystatin domain	PFAM	PF00031	Cystatin	coord: 63..109 e-value: 2.1E-10 score: 40.7
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 63..109 e-value: 3.313E-7 score: 43.6168
IPR006525	Cystatin-related, plant	TIGRFAM	TIGR01638	TIGR01638	coord: 59..109 e-value: 1.4E-8 score: 32.7

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0024094	Lag0024094	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0024094.1.exon1	Lag0024094.1.exon1	exon
Lag0024094.1.exon2	Lag0024094.1.exon2	exon
Lag0024094.1.exon3	Lag0024094.1.exon3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0024094.1	cds.Lag0024094.1	CDS
cds.Lag0024094.1	cds.Lag0024094.1_2	CDS
cds.Lag0024094.1	cds.Lag0024094.1_3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0024094.1	Lag0024094.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity