Lag0024094 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0024094
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCystatin domain-containing protein
Locationchr10: 483398 .. 483935 (+)
RNA-Seq ExpressionLag0024094
SyntenyLag0024094
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTATCCACCCGAATACGTTTGTCCCGAGTCATGTGATGGATGTCATGAACATGACATCCGTGAGATGAATGATGAAGAGCTGGATCTATACTATGAAGCTGTGCATAAAAGCGAGGTTTTCTTTTCAATCCTTTGCTGCAATATTCTTGATTCTCATCTCAAACTCATTACAATATAATTCAAAATTTTCATTTACGACAGGGTTTTGATGTCCCGCATTTTCCTGATTCCTATGCCATTGCTCTGATTGTGCCTATGTCATTTCTAGAAGAGGTTGAAGAATGTGCACAGGAAGCTATTAAACATTACAACAACCAAAATGTATGTATATATGTTTTCATTTTCTGTTTAACTTGTTAGAGGGATCATATATATGAATGTTTTTTTTTCCATATATACAGGGTACAAATTTTGAGGTTGTGAATATTGTGAAGGCGAACAATCAAGCTGCCCGTGGTATCTTATATTTCATCACCTTCGAGGTCAAACCAATTGGAACACCTATGAGAATTGAGAATGTCATCATAGCATAG

mRNA sequence

ATGGCTTATCCACCCGAATACGTTTGTCCCGAGTCATGTGATGGATGTCATGAACATGACATCCGTGAGATGAATGATGAAGAGCTGGATCTATACTATGAAGCTGTGCATAAAAGCGAGGGTTTTGATGTCCCGCATTTTCCTGATTCCTATGCCATTGCTCTGATTGTGCCTATGTCATTTCTAGAAGAGGTTGAAGAATGTGCACAGGAAGCTATTAAACATTACAACAACCAAAATGGTACAAATTTTGAGGTTGTGAATATTGTGAAGGCGAACAATCAAGCTGCCCGTGGTATCTTATATTTCATCACCTTCGAGGTCAAACCAATTGGAACACCTATGAGAATTGAGAATGTCATCATAGCATAG

Coding sequence (CDS)

ATGGCTTATCCACCCGAATACGTTTGTCCCGAGTCATGTGATGGATGTCATGAACATGACATCCGTGAGATGAATGATGAAGAGCTGGATCTATACTATGAAGCTGTGCATAAAAGCGAGGGTTTTGATGTCCCGCATTTTCCTGATTCCTATGCCATTGCTCTGATTGTGCCTATGTCATTTCTAGAAGAGGTTGAAGAATGTGCACAGGAAGCTATTAAACATTACAACAACCAAAATGGTACAAATTTTGAGGTTGTGAATATTGTGAAGGCGAACAATCAAGCTGCCCGTGGTATCTTATATTTCATCACCTTCGAGGTCAAACCAATTGGAACACCTATGAGAATTGAGAATGTCATCATAGCATAG

Protein sequence

MAYPPEYVCPESCDGCHEHDIREMNDEELDLYYEAVHKSEGFDVPHFPDSYAIALIVPMSFLEEVEECAQEAIKHYNNQNGTNFEVVNIVKANNQAARGILYFITFEVKPIGTPMRIENVIIA
Homology
[Close]
BLAST of Lag0024094 vs. NCBI nr
Match: XP_022137488.1 (uncharacterized protein LOC111008920 isoform X1 [Momordica charantia])

HSP 1 Score: 124.8 bits (312), Expect = 5.2e-25
Identity = 58/105 (55.24%), Postives = 80/105 (76.19%), Query Frame = 0 

Query: 14  DGCHEHDIREMNDEELDLYYEAVHKSEGFDVPHFPDSYAIALIVPMSFL----EEVEECA 73
           DG ++  +REMN+EE++ YY+A+ +SEGFDVP FP +YA A+I P+  +    EEV ECA
Sbjct: 46  DGYYDDGVREMNEEEVNRYYKAIQESEGFDVPSFPGTYAFAIISPLPLINLISEEVRECA 105

Query: 74  QEAIKHYNNQNGTNFEVVNIVKANNQAARGILYFITFEVKPIGTP 115
            +AIKHYNN+NG +FE V ++KAN+QAA G L+F+TF+VK  G P
Sbjct: 106 GQAIKHYNNENGASFEFVKMLKANSQAALGELFFLTFQVKQTGAP 150

[Close]
BLAST of Lag0024094 vs. NCBI nr
Match: XP_023525925.1 (uncharacterized protein LOC111789396 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 111.7 bits (278), Expect = 4.6e-21
Identity = 54/107 (50.47%), Postives = 75/107 (70.09%), Query Frame = 0 

Query: 11  ESCDGCHEHDIREMNDEELDLYYEAVHKSEGFDVPHFPDSYAIALIVPM--SFLEE--VE 70
           +  D  +EH +R MN EE+  YY+ +  ++GFDVP FP++YA+ LIVP+   +L +  + 
Sbjct: 47  DDSDEEYEHGVRVMNREEMSRYYQVLRDTDGFDVPTFPNTYALGLIVPICEEYLSKPYLR 106

Query: 71  ECAQEAIKHYNNQNGTNFEVVNIVKANNQAARGILYFITFEVKPIGT 114
           +CA++AI HYN +NGTNFE V IVKAN Q   G  Y+ITF+VK IGT
Sbjct: 107 DCAEKAISHYNIRNGTNFEFVKIVKANQQVVSGFFYYITFDVKQIGT 153

[Close]
BLAST of Lag0024094 vs. NCBI nr
Match: XP_022942206.1 (uncharacterized protein LOC111447330 [Cucurbita moschata] >XP_022942207.1 uncharacterized protein LOC111447330 [Cucurbita moschata])

HSP 1 Score: 109.4 bits (272), Expect = 2.3e-20
Identity = 56/100 (56.00%), Postives = 72/100 (72.00%), Query Frame = 0 

Query: 27  EELDLYYEAVHKSEGFDVPHFPDSYAIALIVPM------SFLEEVEECAQEAIKHYNNQN 86
           EE++ YY AV +S+GFDVP F + YA +LI PM        L+EV+  A EAIKHYNN+N
Sbjct: 33  EEME-YYCAVEESQGFDVPDFGNVYAFSLITPMKLHEVPGMLKEVQISANEAIKHYNNEN 92

Query: 87  GTNFEVVNIVKANNQAARGILYFITFEVKPIGTPMRIENV 121
           GTNFEVV+IVKAN+    G +Y+ITF VKPIGTP+   ++
Sbjct: 93  GTNFEVVDIVKANHSGCCGSMYYITFNVKPIGTPLEFPSI 131

[Close]
BLAST of Lag0024094 vs. NCBI nr
Match: XP_023548353.1 (UPF0725 protein At1g02770-like [Cucurbita pepo subsp. pepo] >XP_023548361.1 UPF0725 protein At1g02770-like [Cucurbita pepo subsp. pepo] >XP_023548367.1 UPF0725 protein At1g02770-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 102.1 bits (253), Expect = 3.6e-18
Identity = 54/93 (58.06%), Postives = 67/93 (72.04%), Query Frame = 0 

Query: 27  EELDLYYEAVHKSEGFDVPHFPDSYAIALIVPM------SFLEEVEECAQEAIKHYNNQN 86
           EE++ YY AV +S+GFDVP F   YA +LI PM      S L+EV+    EAIKHYNN+N
Sbjct: 33  EEME-YYCAVQESQGFDVPDFGTVYAFSLITPMKLHEFPSMLKEVQISPNEAIKHYNNEN 92

Query: 87  GTNFEVVNIVKANNQAARGILYFITFEVKPIGT 114
           GTNFE+V+IVKAN+    G +Y+ITF VKPIGT
Sbjct: 93  GTNFELVDIVKANHSGCCGSMYYITFNVKPIGT 124

[Close]
BLAST of Lag0024094 vs. NCBI nr
Match: XP_023521680.1 (UPF0725 protein At1g02770-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 102.1 bits (253), Expect = 3.6e-18
Identity = 54/93 (58.06%), Postives = 67/93 (72.04%), Query Frame = 0 

Query: 27  EELDLYYEAVHKSEGFDVPHFPDSYAIALIVPM------SFLEEVEECAQEAIKHYNNQN 86
           EE++ YY AV +S+GFDVP F   YA +LI PM      S L+EV+    EAIKHYNN+N
Sbjct: 33  EEME-YYCAVQESQGFDVPDFGTVYAFSLITPMKLHEFPSMLKEVQISPNEAIKHYNNEN 92

Query: 87  GTNFEVVNIVKANNQAARGILYFITFEVKPIGT 114
           GTNFE+V+IVKAN+    G +Y+ITF VKPIGT
Sbjct: 93  GTNFELVDIVKANHSGCCGSMYYITFNVKPIGT 124

The following BLAST results are available for this feature:
BLAST of Lag0024094 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022137488.15.2e-2555.24uncharacterized protein LOC111008920 isoform X1 [Momordica charantia][more]
XP_023525925.14.6e-2150.47uncharacterized protein LOC111789396 [Cucurbita pepo subsp. pepo][more]
XP_022942206.12.3e-2056.00uncharacterized protein LOC111447330 [Cucurbita moschata] >XP_022942207.1 unchar... [more]
XP_023548353.13.6e-1858.06UPF0725 protein At1g02770-like [Cucurbita pepo subsp. pepo] >XP_023548361.1 UPF0... [more]
XP_023521680.13.6e-1858.06UPF0725 protein At1g02770-like [Cucurbita pepo subsp. pepo][more]
BLAST of Lag0024094 vs. NCBI nr
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 59..79
NoneNo IPR availableGENE3D3.10.450.10coord: 58..122
e-value: 7.0E-12
score: 47.5
NoneNo IPR availablePANTHERPTHR31260CYSTATIN/MONELLIN SUPERFAMILY PROTEINcoord: 4..107
NoneNo IPR availablePANTHERPTHR31260:SF28CYSTATIN/MONELLIN SUPERFAMILY PROTEINcoord: 4..107
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 63..109
IPR000010Cystatin domainPFAMPF00031Cystatincoord: 63..109
e-value: 2.1E-10
score: 40.7
IPR000010Cystatin domainCDDcd00042CYcoord: 63..109
e-value: 3.313E-7
score: 43.6168
IPR006525Cystatin-related, plantTIGRFAMTIGR01638TIGR01638coord: 59..109
e-value: 1.4E-8
score: 32.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0024094.1Lag0024094.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010466 negative regulation of peptidase activity
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity