HG10018604.1 (mRNA) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10018604.1
TypemRNA
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionabscisic acid 8'-hydroxylase 4
LocationChr04: 5729414 .. 5733171 (-)
Sequence length1179
RNA-Seq ExpressionHG10018604.1
SyntenyHG10018604.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGCAAGCGCCGCCGCAGCACGGTTCGTTCTGGTGACAAACGCCAATTTATTCCGGCCGACTTACCCCAAAAGTAAAGAGACGATGATCGGACCGGCGGCACTTTTCTTCCACCAAGGAAATTACCATTCTAACCTCAGGAAACTTGTTCAAAATTCTCTCTCTCTTGAACGACTCAGAATTCTTGTTCCTCATATTGAAGCCGCCGCCATTTCCGCCACCCATTCCTGGGCCGCCGCCGGCCACGTCATCAATACCTTCCTCGAAATGAAAAAGGTCCGTCCCCCCTCACATTAAACAAAAATATTCTATAGAAACAGAGAATTTCTCCAAATGATTTTGTTAGTAAGGAATTAAAACTGTGTTCTTATCCAAAATACAAGTGTTTTTAATTTAGTCCAATATATCAATTTTGAATATAGTTCAACTAAATAAAAATTTAAATTCTCACTCTACACATTACATTATTGCACTGAAAATAAAATCTCTTTGACAACTATTTTGTCTTTTTTTTTTTTTTTTTTTTTTTGAAGAAATCTGCTAGTTTTTTTTTTCATATTTTCCTTAAATGATTTTCATCTTAAACATTGAAATTATTAGTCAAATTTCAAACATGAAAGCCTTCTTTTAATTTTTTTTATTTGAAATTTAGCTAAGAATTCAAATATTTATTAAGAAATATGAAAACCATACTAAAAAAATAGCGAGATAACCAACATAAATTTCAAAAACATAAAGCTAACAAATAAACCGTCATCAAACAAAGTCAAAATATATATGAAGTGTATCTTAAGCTGCACCTATCTTTCTGCAGTCCATTTCAACCAAACAGAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTAAAAAAATAGACAATTGGGTGGATTGCAAAAAAGATAAATGCAGTCTAGGATGCACGAAAATCCTCGTATTTGAGTAATGAAAATTGAATGAATTTGAAATTTTTGAACTGATTTTGTGTGTGTATATGTTTGTGTGAACAGTATTCTTTTGAGGTGGGAATTGTAGCAGTGTTTGGTAAGCTAGAAAATGAGTACAAAGAAAAGCTAAAAGAAAAGTATTGCATATTAGACAAAGGCTACAATTGTTTTCCAACCAGACTACCAGGAACACCATATTCAAAAGCATTATCAGTAAGAATTATTCCCAATAATAATCTTCTCAGATTCCCATCATCTATTTTTTTTTTTTTTTTTAATTCTTGACAAGAATAGTTTCATTAAATCTCATAACAGGCAAGGAAGAAGCTTAGGGAGATTCTGGGAGAGATAATTATGGAGAGAAGAGAAAAAAGAGTAACAGAGAGAGATTTAGTGGGTCATCTTTTAAACTTCAGAGATGAAAATGGTCAAAGTTTAAGTGAAGATCAAATCGCCGACAACATCATCGGAGTTCTCTTCGCCGCTCAAGACACCACCGCCACTGCTCTCACCTGGATTCTTAAATATCTTCATGACAACTTGAAGCTCTTTGAGGCTGTTAAGGTAACCATCATTTAATTAATTTTAGGTATTTCCAAATTTGTAACAATTAATTTAGTCTCTGTCCTGAAAAAAAAATGTTATTAAAAGTTAAATGACAAAATAAATTTTAGGAAAATTTTTAAAAATAGAAAAATAAAGAAAACTATTTATACAAAATAGTAAAATTTTTAGATAGTTGTGATAAACGCTGATATAAGTCTATCAAGGTTTATCAGTAATAGAAATGATAGAAATCTATCACTGATAGATGCTAATAGAAGTCTATCAATGTCTATCAATGTTTTTTTTTATCTTCGGTAAATAATTTGACCTTTTTTCTATCTGTGAAAATTTCTCTAAAATTTATTTATAAATTTATAATGATTTTATGGATTAAATGATATTACCCAATACAAGACTTCTTGCTCTCATACCATATTAAGTGTAATTGTTTTAATGATGTTTTCTGTGGTGTGGTTTTTTATTTTTATTTTTTTGTGTAAATTTTTCAGGCAGAGCAAATGGAAATTTATAGAAGAAATGGAGAGGGGAAGATGCCTCTGTCTTGGGCTCAGATTAAGGACATGCCCTTTACTCATAGGGTATTTTTTTTTCCCTTTTGCTTAATTTGATTTTAGAGTTCAGTAACCATTTTGTTTTTTCGTAATTTGGTGTTAAATTCTTTTACCTTCTATACAAACATATTTAAATTTTATACCAAATTTTTAAAAGAAAATTCATGTTCTTAAAAAATTACAAAGGTAGGAATTAAAAGTATACCTCCTTTTAACTTTTCTTTTAATTATTATTATTTGTATCAATTCAGTCTCTAAATATAACTAACGTGGATTATATTCCTATAAAAAAATCAACAAAATAATAAAAATTTTAACCATAAGCCTTAGTTCATATTTAATATGATGATGATATGCATATGGTTATTTTTCAGGTTGTATTAGAGAGCTTGAGGATGGCTAGCATCATATCCTTCACTTTTAGGGAGGCAGTTGTTGATGTGGAATATAAAGGTATATTCCTTGGTTTTTTTTTTAATTAATGAACAAAATCAACATATGGTGAATAAATAAATAATTAATTAATCATATTATATTTAATATATATATTTTTTAATATATGTTTAGGGTACCTTATCCCAAAGGGTTGGAAGGTCATGCCCTTATTTAGGAATATTCATCACAACCATGAGTACTTCCCTGATCCTCATATCTTTGATCCCTCTAGATTTGAGGTAAGCTTTTCAATATTCATATTCTTTTTTTTATATTATTTTATTTAAAATATAACCCAATTTTAATTTAGACTATATAGTTAATTGAAGAAAAAAATGGTTCTAAGAAGTTTATTGTTGTGGGTTATGTAGGTGGCTCCAAGGCCCAATACATTCATGCCATTTGGCAATGGAGTACATTCTTGTCCTGGCAATGAACTTGCCAAGCTGGAGATCCTCATCTTGCTTCATCACCTCATTACCAAATTTAGGTAAATCCAAACCCCAAAATTAAATTGTCATAAAATTAAATTTCTTTACATCAATAATTACAAATCTAACCTTATGTGAGAAAATTAATCTTTCACCTCTTAGATGGCACTATATATAACGTAATTAGTTGAGTTATATTTATATTAACATTTTTTTAAAAAAAATATTTGGATGCATTTTAGGCGATACCTATCTTCATCTAAGTATTTAAGAACATATACTCTCAAAGGAAACTAAATAGATATATTATTATTATTGGGTTAATCTAAACAAAACTCAGTAGATAACTACATGATTAGTTATTGTCTTAAAAGTTGATGGTTCAATTATTTATTTTTGTAATGGTTGAACTCAAAATTATTAGGGCAAAATGAAAAAAAAATATAAAATAAGTAAAATTGTTATTTACACTTAAAATTTGTCAACTCAACATTTGACACATCACTTTTCTGTTAGTCAATTGACGGTCAAATTTAACTTAAGGCCATTTAGAAGTATTTTTCAAATATCGAAGATTAAAGTGTCTGTTTTAAAACTTCAATGACTAAATAAACACTAAATTGAGATCGAAAATGTCTTTTTTTTTCTTCAAATTATTATTATTATGGGAGGTTAATAATAATAATAAAAAATGTGTGTTACTTTTGCAGGTGGGAAGTTGTGGGATCACAAAGTGGGGTTGAGTATGGGCCATTCCCAATGCCTGTCCACGGGCTACCGGCCAGATTTTTCAAACAAACAACAACCAACCAATAG

mRNA sequence

ATGTTGGCAAGCGCCGCCGCAGCACGGTTCGTTCTGGTGACAAACGCCAATTTATTCCGGCCGACTTACCCCAAAAGTAAAGAGACGATGATCGGACCGGCGGCACTTTTCTTCCACCAAGGAAATTACCATTCTAACCTCAGGAAACTTGTTCAAAATTCTCTCTCTCTTGAACGACTCAGAATTCTTGTTCCTCATATTGAAGCCGCCGCCATTTCCGCCACCCATTCCTGGGCCGCCGCCGGCCACGTCATCAATACCTTCCTCGAAATGAAAAAGTATTCTTTTGAGGTGGGAATTGTAGCAGTGTTTGGTAAGCTAGAAAATGAGTACAAAGAAAAGCTAAAAGAAAAGTATTGCATATTAGACAAAGGCTACAATTGTTTTCCAACCAGACTACCAGGAACACCATATTCAAAAGCATTATCAGCAAGGAAGAAGCTTAGGGAGATTCTGGGAGAGATAATTATGGAGAGAAGAGAAAAAAGAGTAACAGAGAGAGATTTAGTGGGTCATCTTTTAAACTTCAGAGATGAAAATGGTCAAAGTTTAAGTGAAGATCAAATCGCCGACAACATCATCGGAGTTCTCTTCGCCGCTCAAGACACCACCGCCACTGCTCTCACCTGGATTCTTAAATATCTTCATGACAACTTGAAGCTCTTTGAGGCTGTTAAGGCAGAGCAAATGGAAATTTATAGAAGAAATGGAGAGGGGAAGATGCCTCTGTCTTGGGCTCAGATTAAGGACATGCCCTTTACTCATAGGGTTGTATTAGAGAGCTTGAGGATGGCTAGCATCATATCCTTCACTTTTAGGGAGGCAGTTGTTGATGTGGAATATAAAGGGTACCTTATCCCAAAGGGTTGGAAGGTCATGCCCTTATTTAGGAATATTCATCACAACCATGAGTACTTCCCTGATCCTCATATCTTTGATCCCTCTAGATTTGAGGTGGCTCCAAGGCCCAATACATTCATGCCATTTGGCAATGGAGTACATTCTTGTCCTGGCAATGAACTTGCCAAGCTGGAGATCCTCATCTTGCTTCATCACCTCATTACCAAATTTAGGTGGGAAGTTGTGGGATCACAAAGTGGGGTTGAGTATGGGCCATTCCCAATGCCTGTCCACGGGCTACCGGCCAGATTTTTCAAACAAACAACAACCAACCAATAG

Coding sequence (CDS)

ATGTTGGCAAGCGCCGCCGCAGCACGGTTCGTTCTGGTGACAAACGCCAATTTATTCCGGCCGACTTACCCCAAAAGTAAAGAGACGATGATCGGACCGGCGGCACTTTTCTTCCACCAAGGAAATTACCATTCTAACCTCAGGAAACTTGTTCAAAATTCTCTCTCTCTTGAACGACTCAGAATTCTTGTTCCTCATATTGAAGCCGCCGCCATTTCCGCCACCCATTCCTGGGCCGCCGCCGGCCACGTCATCAATACCTTCCTCGAAATGAAAAAGTATTCTTTTGAGGTGGGAATTGTAGCAGTGTTTGGTAAGCTAGAAAATGAGTACAAAGAAAAGCTAAAAGAAAAGTATTGCATATTAGACAAAGGCTACAATTGTTTTCCAACCAGACTACCAGGAACACCATATTCAAAAGCATTATCAGCAAGGAAGAAGCTTAGGGAGATTCTGGGAGAGATAATTATGGAGAGAAGAGAAAAAAGAGTAACAGAGAGAGATTTAGTGGGTCATCTTTTAAACTTCAGAGATGAAAATGGTCAAAGTTTAAGTGAAGATCAAATCGCCGACAACATCATCGGAGTTCTCTTCGCCGCTCAAGACACCACCGCCACTGCTCTCACCTGGATTCTTAAATATCTTCATGACAACTTGAAGCTCTTTGAGGCTGTTAAGGCAGAGCAAATGGAAATTTATAGAAGAAATGGAGAGGGGAAGATGCCTCTGTCTTGGGCTCAGATTAAGGACATGCCCTTTACTCATAGGGTTGTATTAGAGAGCTTGAGGATGGCTAGCATCATATCCTTCACTTTTAGGGAGGCAGTTGTTGATGTGGAATATAAAGGGTACCTTATCCCAAAGGGTTGGAAGGTCATGCCCTTATTTAGGAATATTCATCACAACCATGAGTACTTCCCTGATCCTCATATCTTTGATCCCTCTAGATTTGAGGTGGCTCCAAGGCCCAATACATTCATGCCATTTGGCAATGGAGTACATTCTTGTCCTGGCAATGAACTTGCCAAGCTGGAGATCCTCATCTTGCTTCATCACCTCATTACCAAATTTAGGTGGGAAGTTGTGGGATCACAAAGTGGGGTTGAGTATGGGCCATTCCCAATGCCTGTCCACGGGCTACCGGCCAGATTTTTCAAACAAACAACAACCAACCAATAG

Protein sequence

MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERLRILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYCILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDENGQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGKMPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVVGSQSGVEYGPFPMPVHGLPARFFKQTTTNQ
Homology
BLAST of HG10018604.1 vs. NCBI nr
Match: XP_038887333.1 (abscisic acid 8'-hydroxylase 4 [Benincasa hispida])

HSP 1 Score: 767.7 bits (1981), Expect = 4.9e-218
Identity = 377/392 (96.17%), Postives = 386/392 (98.47%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLASAAAARFVLVTNA+LFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL
Sbjct: 91  MLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 150

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           RILVPHIEAAAISAT SWAAAGHVINTFLEMKKYSFEVGIVAVF KLENEYKEKLKEKY 
Sbjct: 151 RILVPHIEAAAISATDSWAAAGHVINTFLEMKKYSFEVGIVAVFEKLENEYKEKLKEKYS 210

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           ILDKGYNCFPTRLPGT YSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN
Sbjct: 211 ILDKGYNCFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 270

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
           G++LSEDQIADNIIGVLFAAQDTTATALTWILK+LHDNLKLFEAVKAEQMEIYRRNG+GK
Sbjct: 271 GETLSEDQIADNIIGVLFAAQDTTATALTWILKFLHDNLKLFEAVKAEQMEIYRRNGDGK 330

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
           MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVD+EYKGYLIPKGWKVMPLFRNIH
Sbjct: 331 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDMEYKGYLIPKGWKVMPLFRNIH 390

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           HN EYFPDPHIFDPSRFE+AP+PNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE
Sbjct: 391 HNPEYFPDPHIFDPSRFELAPKPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 450

Query: 361 VVGSQSGVEYGPFPMPVHGLPARFFKQTTTNQ 393
           VVGS+SGVEYGPFPMPVHGLPARFFK TTTNQ
Sbjct: 451 VVGSESGVEYGPFPMPVHGLPARFFKHTTTNQ 482

BLAST of HG10018604.1 vs. NCBI nr
Match: XP_008454947.1 (PREDICTED: abscisic acid 8'-hydroxylase 4 [Cucumis melo])

HSP 1 Score: 761.5 bits (1965), Expect = 3.5e-216
Identity = 373/392 (95.15%), Postives = 380/392 (96.94%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLASAAAARFVLVTNA+LFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLV NSLSLERL
Sbjct: 89  MLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERL 148

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R LVP IEAAAISAT SWAAAGHVINTF EMKKYSFEVGI+AVFGKLENEYKEKLK+KYC
Sbjct: 149 RTLVPSIEAAAISATDSWAAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYC 208

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           ILDKGYNCFPTRLPGT YSKALSARKKLREILGEIIMERREKRVTERD VGHLLN+RDEN
Sbjct: 209 ILDKGYNCFPTRLPGTGYSKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDEN 268

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
           G+SLSEDQIADNIIGVLFAAQDTTAT LTWILKYLHDN KLFEAVKAEQMEIYRRNGEGK
Sbjct: 269 GESLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGK 328

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
           MPLSW QIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 329 MPLSWGQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 388

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE
Sbjct: 389 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 448

Query: 361 VVGSQSGVEYGPFPMPVHGLPARFFKQTTTNQ 393
           V GSQSGVEYGPFPMP+ GLP+RFFKQTTTNQ
Sbjct: 449 VEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 480

BLAST of HG10018604.1 vs. NCBI nr
Match: KAA0031296.1 (abscisic acid 8'-hydroxylase 4 [Cucumis melo var. makuwa] >TYK06748.1 abscisic acid 8'-hydroxylase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 761.5 bits (1965), Expect = 3.5e-216
Identity = 373/392 (95.15%), Postives = 380/392 (96.94%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLASAAAARFVLVTNA+LFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLV NSLSLERL
Sbjct: 43  MLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERL 102

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R LVP IEAAAISAT SWAAAGHVINTF EMKKYSFEVGI+AVFGKLENEYKEKLK+KYC
Sbjct: 103 RTLVPSIEAAAISATDSWAAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYC 162

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           ILDKGYNCFPTRLPGT YSKALSARKKLREILGEIIMERREKRVTERD VGHLLN+RDEN
Sbjct: 163 ILDKGYNCFPTRLPGTGYSKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDEN 222

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
           G+SLSEDQIADNIIGVLFAAQDTTAT LTWILKYLHDN KLFEAVKAEQMEIYRRNGEGK
Sbjct: 223 GESLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGK 282

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
           MPLSW QIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 283 MPLSWGQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 342

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE
Sbjct: 343 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 402

Query: 361 VVGSQSGVEYGPFPMPVHGLPARFFKQTTTNQ 393
           V GSQSGVEYGPFPMP+ GLP+RFFKQTTTNQ
Sbjct: 403 VEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 434

BLAST of HG10018604.1 vs. NCBI nr
Match: XP_004137112.1 (abscisic acid 8'-hydroxylase 4 [Cucumis sativus] >KAE8645865.1 hypothetical protein Csa_017084 [Cucumis sativus])

HSP 1 Score: 760.8 bits (1963), Expect = 6.0e-216
Identity = 374/392 (95.41%), Postives = 381/392 (97.19%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLASAAAARFVLVTNA+LFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLV NSLSLERL
Sbjct: 89  MLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERL 148

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R LVP IEAAAISAT SWAAAGHVINTFLEMKKYSFEVGI+AVFGKLE+EYKEKLK+ YC
Sbjct: 149 RTLVPCIEAAAISATDSWAAAGHVINTFLEMKKYSFEVGIIAVFGKLEDEYKEKLKQNYC 208

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           ILDKGYNCFPTRLPGT YSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN
Sbjct: 209 ILDKGYNCFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 268

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
           G++LSEDQIADNIIGVLFAAQDTTAT LTWILKYLHDN KLFEAVKAEQMEIYRRNGEGK
Sbjct: 269 GENLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGK 328

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
           MPLSW+QIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 329 MPLSWSQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 388

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           HNHEYFPDPHIFDPSRFEVAPR NTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE
Sbjct: 389 HNHEYFPDPHIFDPSRFEVAPRANTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 448

Query: 361 VVGSQSGVEYGPFPMPVHGLPARFFKQTTTNQ 393
           V GSQSGVEYGPFPMPV GLPARFFKQTTTNQ
Sbjct: 449 VEGSQSGVEYGPFPMPVQGLPARFFKQTTTNQ 480

BLAST of HG10018604.1 vs. NCBI nr
Match: XP_022927667.1 (abscisic acid 8'-hydroxylase 4-like [Cucurbita moschata])

HSP 1 Score: 731.5 bits (1887), Expect = 3.9e-207
Identity = 358/392 (91.33%), Postives = 375/392 (95.66%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLASAAAARFVLVTNA+LFRPTYPKSKETMIGPAALFFHQGNYHSN+RKLVQNSL L  L
Sbjct: 84  MLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNIRKLVQNSLCLGSL 143

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R LVPHIE AAISA  SWAAAGHVINTFLEMKK+SF+VGI+AVFGKLE++Y EKLKE YC
Sbjct: 144 RNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFDVGILAVFGKLESDYSEKLKENYC 203

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           ILDKGYN FPTRLPGT YSKALSARKKLREILGEIIMERREKRVTERDLVGHLL+FRDEN
Sbjct: 204 ILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLSFRDEN 263

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
           G  LSEDQIADNIIGVLFAAQDTTA+ LTWILKYLHDNLKLFEAV+AEQMEIYRRNG+GK
Sbjct: 264 GGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHDNLKLFEAVRAEQMEIYRRNGDGK 323

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
           MPLSWAQIKDMP THRVVLESLRMASIISFTFREAVVDVEYKG+LIPKGWKVMPLFRNIH
Sbjct: 324 MPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVVDVEYKGFLIPKGWKVMPLFRNIH 383

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           HN +YFP+P IFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEIL+LLHHLITKFRWE
Sbjct: 384 HNPDYFPNPQIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILVLLHHLITKFRWE 443

Query: 361 VVGSQSGVEYGPFPMPVHGLPARFFKQTTTNQ 393
           VVGSQSGV+YGPFPMPV+GLPARFFKQTTTNQ
Sbjct: 444 VVGSQSGVQYGPFPMPVNGLPARFFKQTTTNQ 475

BLAST of HG10018604.1 vs. ExPASy Swiss-Prot
Match: Q9LJK2 (Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 SV=2)

HSP 1 Score: 550.1 bits (1416), Expect = 2.1e-155
Identity = 260/384 (67.71%), Postives = 319/384 (83.07%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLAS  AARFVLVT+A++F+PTYP+SKE +IGP+ALFFHQG+YHS++RKLVQ+S   E +
Sbjct: 81  MLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSFYPETI 140

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R L+P IE  A+S+  SWA    +++T+ EMKK++F+VGI+A+FG LE+ YKE LK  Y 
Sbjct: 141 RKLIPDIEHIALSSLQSWANM-PIVSTYQEMKKFAFDVGILAIFGHLESSYKEILKHNYN 200

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           I+DKGYN FP  LPGT Y KAL ARK+L+ I+ EII ERREKR  + D +GHLLNF++E 
Sbjct: 201 IVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRALQTDFLGHLLNFKNEK 260

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
           G+ L+++QIADNIIGVLFAAQDTTA+ LTWILKYLHD+ KL EAVKAEQ  IY  N   K
Sbjct: 261 GRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQKAIYEENSREK 320

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
            PL+W Q ++MP TH+V++ESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 321 KPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 380

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           HN +YF +P +FDPSRFEV P+PNTFMPFG+GVH+CPGNELAKL+ILI LHHL++ FRWE
Sbjct: 381 HNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNELAKLQILIFLHHLVSNFRWE 440

Query: 361 VVGSQSGVEYGPFPMPVHGLPARF 385
           V G + G++Y PFP+P +GLPA F
Sbjct: 441 VKGGEKGIQYSPFPIPQNGLPATF 463

BLAST of HG10018604.1 vs. ExPASy Swiss-Prot
Match: A9QNE7 (Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A1 PE=2 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 1.5e-153
Identity = 267/391 (68.29%), Postives = 317/391 (81.07%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLAS  AARFVLV  ANLF+PTYPKSKE +IG +A+FFHQG+YH++LRKLVQ  L+ E +
Sbjct: 87  MLASPEAARFVLVNQANLFKPTYPKSKENLIGQSAIFFHQGDYHNHLRKLVQAPLNPESI 146

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R  +P+IE  +ISA +SW   GHV+NT+ EMKK+SFEVGI+A+FG L+   KE+LK+ Y 
Sbjct: 147 RNQIPYIEELSISALNSW-VGGHVVNTYHEMKKFSFEVGILAIFGHLDGHVKEELKKNYS 206

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           I+DKGYN FP  LPGT Y KAL ARKKL +IL EII E +EK+  E+ L+   LN ++E 
Sbjct: 207 IVDKGYNSFPINLPGTLYRKALQARKKLGKILSEIIREMKEKKTLEKGLLSCFLNAKEEK 266

Query: 181 G-QSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEG 240
           G   L+EDQIADNIIGVLFAAQDTTA+ LTWI+KYLHDN KL E VKAEQ  I++ N + 
Sbjct: 267 GFLVLNEDQIADNIIGVLFAAQDTTASVLTWIIKYLHDNPKLLECVKAEQKVIWQSNEQE 326

Query: 241 KMPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNI 300
              L+W Q + MP T RVVLE+LRMASIISF FREAV DVEYKGYLIPKGWKVMPLFRNI
Sbjct: 327 NHGLTWTQTRKMPITSRVVLETLRMASIISFAFREAVADVEYKGYLIPKGWKVMPLFRNI 386

Query: 301 HHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRW 360
           HHN E+FPDP  FDPSRFE AP+PNTFMPFG+GVH+CPGNELAKLEILI+ HHL+TKFRW
Sbjct: 387 HHNPEFFPDPQKFDPSRFENAPKPNTFMPFGSGVHACPGNELAKLEILIMTHHLVTKFRW 446

Query: 361 EVVGSQSGVEYGPFPMPVHGLPARFFKQTTT 391
           EVVGS SG++YGPFP+P+ GL ARF+K T+T
Sbjct: 447 EVVGSGSGIQYGPFPVPLGGLAARFWKTTST 476

BLAST of HG10018604.1 vs. ExPASy Swiss-Prot
Match: Q09J78 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 4.5e-142
Identity = 246/392 (62.76%), Postives = 305/392 (77.81%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLAS  AARFVLV+ A LF+PTYP SKE MIGP+ALFFHQG YH  LR+LVQ +L+ + L
Sbjct: 98  MLASPEAARFVLVSQARLFKPTYPPSKERMIGPSALFFHQGEYHLRLRRLVQAALAPDSL 157

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVF-GKLENEYKEKLKEKY 120
           R LVP ++AA  +   +W + GHV +TF  MKK SF+VG+V +F G+L   ++E+L+  Y
Sbjct: 158 RALVPDVDAAVAATLAAW-SGGHVASTFHAMKKLSFDVGVVTIFGGRLGRRHREELRTNY 217

Query: 121 CILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDE 180
            ++++GYNCFP R PGT Y KA+ ARK+LR IL EI+ ERR +     DL+G L+  RD+
Sbjct: 218 SVVERGYNCFPNRFPGTLYHKAIQARKRLRAILSEIVAERRARGGGGDDLLGGLMRSRDD 277

Query: 181 NGQS----LSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRR 240
                   L++DQIADN++GVLFAAQDTTA+ LTWILKYLHD+ KL EAVKAEQM IY  
Sbjct: 278 GTAGAVALLTDDQIADNVVGVLFAAQDTTASVLTWILKYLHDSPKLLEAVKAEQMAIYVA 337

Query: 241 NGEGKMPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPL 300
           N  GK PL+W Q + M  TH+V+LESLRMASIISFTFREAV DVEYKG+LIPKGWKVMPL
Sbjct: 338 NEGGKRPLTWTQTRSMTLTHQVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPL 397

Query: 301 FRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLIT 360
           FRNIHHN +YF DP  FDPSRF+VAPRP+TF+PFG+GVH+CPGNELAKLE+L+L+H L+T
Sbjct: 398 FRNIHHNPDYFQDPQKFDPSRFKVAPRPSTFLPFGSGVHACPGNELAKLEMLVLVHRLVT 457

Query: 361 KFRWEVVGSQSGVEYGPFPMPVHGLPARFFKQ 388
            +RWE+VG+   VEY PFP+P  GL A+ +KQ
Sbjct: 458 AYRWEIVGASDEVEYSPFPVPRGGLNAKLWKQ 488

BLAST of HG10018604.1 vs. ExPASy Swiss-Prot
Match: Q0J185 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A7 PE=2 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 3.8e-141
Identity = 245/393 (62.34%), Postives = 306/393 (77.86%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLAS  AARFVLVT A+LF+PTYP+SKE MIGP+ALFFHQG+YH  LRKLVQ  L  + L
Sbjct: 91  MLASPEAARFVLVTQAHLFKPTYPRSKERMIGPSALFFHQGDYHLRLRKLVQGPLGPDAL 150

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVF-GKLENEYKEKLKEKY 120
           R LVP +EAA  S   SW   G+V +TF  MK+ SF+VGIV +F G+L+   K +L++ Y
Sbjct: 151 RALVPDVEAAVRSTLASW--DGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNY 210

Query: 121 CILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFR-D 180
            I++KGYN FP   PGT Y KA+ AR++L  +L +I+ ERR +     DL+G L+  R  
Sbjct: 211 AIVEKGYNSFPNSFPGTLYYKAIQARRRLHGVLSDIMRERRARGEPGSDLLGCLMQSRAG 270

Query: 181 ENGQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGE 240
           ++G  L+++Q+ADNIIGVLFAAQDTTA+ LTWI+KYLHD+ KL EAV+AEQ  I   N  
Sbjct: 271 DDGALLTDEQVADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDG 330

Query: 241 GKMPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRN 300
           G++PL+WAQ + M  TH+V+LESLRMASIISFTFREAV DVEYKG+LIPKGWKVMPLFRN
Sbjct: 331 GRLPLTWAQTRSMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRN 390

Query: 301 IHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFR 360
           IHHN +YF DP  FDPSRF+V+PRPNTFMPFGNGVH+CPGNELAKLE+L+L+HHL+T +R
Sbjct: 391 IHHNPDYFQDPQKFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYR 450

Query: 361 WEVVGSQSGVEYGPFPMPVHGLPARFFKQTTTN 392
           WE+VGS   VEY PFP+P HGL A+ ++  + +
Sbjct: 451 WEIVGSSDEVEYSPFPVPKHGLLAKLWRDDSVS 481

BLAST of HG10018604.1 vs. ExPASy Swiss-Prot
Match: A2Z212 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A7 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 8.5e-141
Identity = 245/393 (62.34%), Postives = 306/393 (77.86%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLAS  AARFVLVT A+LF+PTYP+SKE MIGP+ALFF+QG+YH  LRKLVQ  L  + L
Sbjct: 91  MLASPEAARFVLVTQAHLFKPTYPRSKERMIGPSALFFNQGDYHLRLRKLVQGPLGPDAL 150

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVF-GKLENEYKEKLKEKY 120
           R LVP +EAA  S   SW   G+V +TF  MK+ SF+VGIV +F G+L+   K +L++ Y
Sbjct: 151 RALVPDVEAAVRSTLASW--DGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNY 210

Query: 121 CILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFR-D 180
            I++KGYN FP   PGT Y KA+ AR++L  +L +I+ ERR +     DL+G L+  R  
Sbjct: 211 AIVEKGYNSFPNSFPGTLYYKAIQARRRLHGVLSDIMRERRARGEPGSDLLGCLMQSRAG 270

Query: 181 ENGQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGE 240
           ++G  L+++Q+ADNIIGVLFAAQDTTA+ LTWI+KYLHD+ KL EAV+AEQ  I   N  
Sbjct: 271 DDGALLTDEQVADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDG 330

Query: 241 GKMPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRN 300
           G++PL+WAQ + M  TH+V+LESLRMASIISFTFREAV DVEYKG+LIPKGWKVMPLFRN
Sbjct: 331 GRLPLTWAQTRSMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRN 390

Query: 301 IHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFR 360
           IHHN +YF DP  FDPSRF+V+PRPNTFMPFGNGVH+CPGNELAKLE+L+L+HHL+T +R
Sbjct: 391 IHHNPDYFQDPQKFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYR 450

Query: 361 WEVVGSQSGVEYGPFPMPVHGLPARFFKQTTTN 392
           WE+VGS   VEY PFP+P HGL A+ ++  T +
Sbjct: 451 WEIVGSSDEVEYSPFPVPKHGLLAKLWRDDTVS 481

BLAST of HG10018604.1 vs. ExPASy TrEMBL
Match: A0A5A7SMJ8 (Abscisic acid 8'-hydroxylase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00490 PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 1.7e-216
Identity = 373/392 (95.15%), Postives = 380/392 (96.94%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLASAAAARFVLVTNA+LFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLV NSLSLERL
Sbjct: 43  MLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERL 102

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R LVP IEAAAISAT SWAAAGHVINTF EMKKYSFEVGI+AVFGKLENEYKEKLK+KYC
Sbjct: 103 RTLVPSIEAAAISATDSWAAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYC 162

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           ILDKGYNCFPTRLPGT YSKALSARKKLREILGEIIMERREKRVTERD VGHLLN+RDEN
Sbjct: 163 ILDKGYNCFPTRLPGTGYSKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDEN 222

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
           G+SLSEDQIADNIIGVLFAAQDTTAT LTWILKYLHDN KLFEAVKAEQMEIYRRNGEGK
Sbjct: 223 GESLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGK 282

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
           MPLSW QIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 283 MPLSWGQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 342

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE
Sbjct: 343 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 402

Query: 361 VVGSQSGVEYGPFPMPVHGLPARFFKQTTTNQ 393
           V GSQSGVEYGPFPMP+ GLP+RFFKQTTTNQ
Sbjct: 403 VEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 434

BLAST of HG10018604.1 vs. ExPASy TrEMBL
Match: A0A1S3BZQ3 (abscisic acid 8'-hydroxylase 4 OS=Cucumis melo OX=3656 GN=LOC103495236 PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 1.7e-216
Identity = 373/392 (95.15%), Postives = 380/392 (96.94%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLASAAAARFVLVTNA+LFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLV NSLSLERL
Sbjct: 89  MLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERL 148

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R LVP IEAAAISAT SWAAAGHVINTF EMKKYSFEVGI+AVFGKLENEYKEKLK+KYC
Sbjct: 149 RTLVPSIEAAAISATDSWAAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYC 208

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           ILDKGYNCFPTRLPGT YSKALSARKKLREILGEIIMERREKRVTERD VGHLLN+RDEN
Sbjct: 209 ILDKGYNCFPTRLPGTGYSKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDEN 268

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
           G+SLSEDQIADNIIGVLFAAQDTTAT LTWILKYLHDN KLFEAVKAEQMEIYRRNGEGK
Sbjct: 269 GESLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGK 328

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
           MPLSW QIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 329 MPLSWGQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 388

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE
Sbjct: 389 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 448

Query: 361 VVGSQSGVEYGPFPMPVHGLPARFFKQTTTNQ 393
           V GSQSGVEYGPFPMP+ GLP+RFFKQTTTNQ
Sbjct: 449 VEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 480

BLAST of HG10018604.1 vs. ExPASy TrEMBL
Match: A0A0A0K6K8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073680 PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 2.9e-216
Identity = 374/392 (95.41%), Postives = 381/392 (97.19%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLASAAAARFVLVTNA+LFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLV NSLSLERL
Sbjct: 43  MLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERL 102

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R LVP IEAAAISAT SWAAAGHVINTFLEMKKYSFEVGI+AVFGKLE+EYKEKLK+ YC
Sbjct: 103 RTLVPCIEAAAISATDSWAAAGHVINTFLEMKKYSFEVGIIAVFGKLEDEYKEKLKQNYC 162

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           ILDKGYNCFPTRLPGT YSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN
Sbjct: 163 ILDKGYNCFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 222

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
           G++LSEDQIADNIIGVLFAAQDTTAT LTWILKYLHDN KLFEAVKAEQMEIYRRNGEGK
Sbjct: 223 GENLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGK 282

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
           MPLSW+QIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 283 MPLSWSQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 342

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           HNHEYFPDPHIFDPSRFEVAPR NTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE
Sbjct: 343 HNHEYFPDPHIFDPSRFEVAPRANTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 402

Query: 361 VVGSQSGVEYGPFPMPVHGLPARFFKQTTTNQ 393
           V GSQSGVEYGPFPMPV GLPARFFKQTTTNQ
Sbjct: 403 VEGSQSGVEYGPFPMPVQGLPARFFKQTTTNQ 434

BLAST of HG10018604.1 vs. ExPASy TrEMBL
Match: A0A6J1EHU3 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111434488 PE=3 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 1.9e-207
Identity = 358/392 (91.33%), Postives = 375/392 (95.66%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLASAAAARFVLVTNA+LFRPTYPKSKETMIGPAALFFHQGNYHSN+RKLVQNSL L  L
Sbjct: 84  MLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNIRKLVQNSLCLGSL 143

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R LVPHIE AAISA  SWAAAGHVINTFLEMKK+SF+VGI+AVFGKLE++Y EKLKE YC
Sbjct: 144 RNLVPHIETAAISAAESWAAAGHVINTFLEMKKFSFDVGILAVFGKLESDYSEKLKENYC 203

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           ILDKGYN FPTRLPGT YSKALSARKKLREILGEIIMERREKRVTERDLVGHLL+FRDEN
Sbjct: 204 ILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLSFRDEN 263

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
           G  LSEDQIADNIIGVLFAAQDTTA+ LTWILKYLHDNLKLFEAV+AEQMEIYRRNG+GK
Sbjct: 264 GGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHDNLKLFEAVRAEQMEIYRRNGDGK 323

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
           MPLSWAQIKDMP THRVVLESLRMASIISFTFREAVVDVEYKG+LIPKGWKVMPLFRNIH
Sbjct: 324 MPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVVDVEYKGFLIPKGWKVMPLFRNIH 383

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           HN +YFP+P IFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEIL+LLHHLITKFRWE
Sbjct: 384 HNPDYFPNPQIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILVLLHHLITKFRWE 443

Query: 361 VVGSQSGVEYGPFPMPVHGLPARFFKQTTTNQ 393
           VVGSQSGV+YGPFPMPV+GLPARFFKQTTTNQ
Sbjct: 444 VVGSQSGVQYGPFPMPVNGLPARFFKQTTTNQ 475

BLAST of HG10018604.1 vs. ExPASy TrEMBL
Match: A0A6J1JMR7 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111486079 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 5.1e-205
Identity = 353/392 (90.05%), Postives = 374/392 (95.41%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLASAAAARFVLVTNA+LFRPTYPKSKETMIGPAALFFHQGNYHSN+RKLVQNSL L  L
Sbjct: 84  MLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNIRKLVQNSLCLGSL 143

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R LVPHIE AAISA  SWAAAGHVINTFLEMKK++F+VGI+AVFGKLE++Y EKLKE YC
Sbjct: 144 RNLVPHIETAAISAAESWAAAGHVINTFLEMKKFAFDVGILAVFGKLESDYSEKLKENYC 203

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           ILDKGYN FPTRLPGT YSKALSARKKLREILGEIIMERREKRVTERDLVGHLL+FRDEN
Sbjct: 204 ILDKGYNSFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLSFRDEN 263

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
           G  LSEDQIADNIIGVLFAAQDTTA+ LTWILKYLHDNLKLFEAV+AEQM+IYRRNG+GK
Sbjct: 264 GGILSEDQIADNIIGVLFAAQDTTASVLTWILKYLHDNLKLFEAVRAEQMDIYRRNGDGK 323

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
           MPLSWAQIKDMP THRVVLESLRMASIISFTFREAVVDVEYKG+LIPKGWKVMPLFRNIH
Sbjct: 324 MPLSWAQIKDMPLTHRVVLESLRMASIISFTFREAVVDVEYKGFLIPKGWKVMPLFRNIH 383

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           H+ +YFP+P IFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHL+TKFRWE
Sbjct: 384 HSPDYFPNPQIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLLTKFRWE 443

Query: 361 VVGSQSGVEYGPFPMPVHGLPARFFKQTTTNQ 393
           VVGSQSGV+YGPFP+PV+GLPARFFKQTT NQ
Sbjct: 444 VVGSQSGVQYGPFPVPVNGLPARFFKQTTANQ 475

BLAST of HG10018604.1 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 550.1 bits (1416), Expect = 1.5e-156
Identity = 260/384 (67.71%), Postives = 319/384 (83.07%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           MLAS  AARFVLVT+A++F+PTYP+SKE +IGP+ALFFHQG+YHS++RKLVQ+S   E +
Sbjct: 81  MLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSFYPETI 140

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R L+P IE  A+S+  SWA    +++T+ EMKK++F+VGI+A+FG LE+ YKE LK  Y 
Sbjct: 141 RKLIPDIEHIALSSLQSWANM-PIVSTYQEMKKFAFDVGILAIFGHLESSYKEILKHNYN 200

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           I+DKGYN FP  LPGT Y KAL ARK+L+ I+ EII ERREKR  + D +GHLLNF++E 
Sbjct: 201 IVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRALQTDFLGHLLNFKNEK 260

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
           G+ L+++QIADNIIGVLFAAQDTTA+ LTWILKYLHD+ KL EAVKAEQ  IY  N   K
Sbjct: 261 GRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQKAIYEENSREK 320

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
            PL+W Q ++MP TH+V++ESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 321 KPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 380

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           HN +YF +P +FDPSRFEV P+PNTFMPFG+GVH+CPGNELAKL+ILI LHHL++ FRWE
Sbjct: 381 HNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNELAKLQILIFLHHLVSNFRWE 440

Query: 361 VVGSQSGVEYGPFPMPVHGLPARF 385
           V G + G++Y PFP+P +GLPA F
Sbjct: 441 VKGGEKGIQYSPFPIPQNGLPATF 463

BLAST of HG10018604.1 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 451.4 bits (1160), Expect = 7.2e-127
Identity = 219/381 (57.48%), Postives = 285/381 (74.80%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           M++S  AA+FVLVT ++LF+PT+P SKE M+G  A+FFHQG+YHS LRKLV  +   + +
Sbjct: 83  MISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLRAFMPDAI 142

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R +VPHIE+ A  + +SW   G  +NT+ EMK Y+F V ++++ GK E  Y+E LK  Y 
Sbjct: 143 RNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNVALISILGKDEVYYREDLKRCYY 202

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           IL+KGYN  P  LPGT + KA+ ARK+L +IL  I+ +RR+   +  DL+G  +    E+
Sbjct: 203 ILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPSSHTDLLGSFM----ED 262

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
              L+++QIADNIIGV+FAA+DTTA+ LTWILKYL DN  + EAV  EQM I +   EG+
Sbjct: 263 KAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGE 322

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
             L+W   K MP T+RV+ E+LR A+I+SFTFREAV DVEY+GYLIPKGWKV+PLFRNIH
Sbjct: 323 -SLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIH 382

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           HN + F DP  FDPSRFEVAP+PNTFMPFG+G+HSCPGNELAKLEI +L+HHL TK+RW 
Sbjct: 383 HNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEISVLIHHLTTKYRWS 442

Query: 361 VVGSQSGVEYGPFPMPVHGLP 382
           +VG   G++YGPF +P +GLP
Sbjct: 443 IVGPSDGIQYGPFALPQNGLP 456

BLAST of HG10018604.1 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 444.5 bits (1142), Expect = 8.8e-125
Identity = 215/381 (56.43%), Postives = 285/381 (74.80%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           M++S  AA+FVLVT ++LF+PT+P SKE M+G  A+FFHQG+YH+ LRKLV  +   E +
Sbjct: 83  MISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESI 142

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFGKLENEYKEKLKEKYC 120
           R +VP IE+ A  +  SW   G +INT+ EMK Y+F V ++++FGK E  Y+E LK  Y 
Sbjct: 143 RNMVPDIESIAQDSLRSW--EGTMINTYQEMKTYTFNVALLSIFGKDEVLYREDLKRCYY 202

Query: 121 ILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDEN 180
           IL+KGYN  P  LPGT + K++ ARK+L +IL  I+ ERR+   +  DL+G  +  ++E 
Sbjct: 203 ILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFMGDKEE- 262

Query: 181 GQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEGK 240
              L+++QIADNIIGV+FAA+DTTA+ ++WILKYL +N  + EAV  EQM I +   EG+
Sbjct: 263 ---LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGE 322

Query: 241 MPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 300
             L+W   K MP T RV+ E+LR+ASI+SFTFREAV DVEY+GYLIPKGWKV+PLFRNIH
Sbjct: 323 -SLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIH 382

Query: 301 HNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWE 360
           H+ + F +P  FDPSRFEVAP+PNTFMPFGNG HSCPGNELAKLE+ I++HHL TK+ W 
Sbjct: 383 HSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWS 442

Query: 361 VVGSQSGVEYGPFPMPVHGLP 382
           +VG+  G++YGPF +P +GLP
Sbjct: 443 IVGASDGIQYGPFALPQNGLP 456

BLAST of HG10018604.1 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 435.3 bits (1118), Expect = 5.3e-122
Identity = 218/384 (56.77%), Postives = 274/384 (71.35%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           M++S  AAR VLV+ A+LF+PTYP SKE MIGP ALFFHQG YHS L++LVQ+S     L
Sbjct: 96  MISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSAL 155

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFG-KLENEYKEKLKEKY 120
           R  V HIE   +    SW +    INT   MK+Y+F+V I++ FG K E    + +K  Y
Sbjct: 156 RPTVSHIELLVLQTLSSWTSQ-KSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLY 215

Query: 121 CILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDE 180
             L++GYN  P  LPGT + K++ AR +L E L ++I +RRE    E  L+G LL  +D+
Sbjct: 216 QRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEGGLLGVLLGAKDQ 275

Query: 181 NGQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEG 240
               LS+ QIADNIIGV+FAA DTTA+ LTW+LKYLHD+  L + V  EQ  I ++  + 
Sbjct: 276 KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKE 335

Query: 241 KMPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNI 300
              +SW   + MP T RV+ E+LR AS++SFTFREAV DVEY GYLIPKGWKV+PLFR I
Sbjct: 336 NRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRI 395

Query: 301 HHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRW 360
           HH+ E+FPDP  FDPSRFEVAP+P T+MPFGNGVHSCPG+ELAKLE+LILLHHL T FRW
Sbjct: 396 HHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRW 455

Query: 361 EVVGSQSGVEYGPFPMPVHGLPAR 384
           EV+G + G++YGPFP+P  GLP R
Sbjct: 456 EVIGDEEGIQYGPFPVPKKGLPIR 478

BLAST of HG10018604.1 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 435.3 bits (1118), Expect = 5.3e-122
Identity = 218/384 (56.77%), Postives = 274/384 (71.35%), Query Frame = 0

Query: 1   MLASAAAARFVLVTNANLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVQNSLSLERL 60
           M++S  AAR VLV+ A+LF+PTYP SKE MIGP ALFFHQG YHS L++LVQ+S     L
Sbjct: 96  MISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSAL 155

Query: 61  RILVPHIEAAAISATHSWAAAGHVINTFLEMKKYSFEVGIVAVFG-KLENEYKEKLKEKY 120
           R  V HIE   +    SW +    INT   MK+Y+F+V I++ FG K E    + +K  Y
Sbjct: 156 RPTVSHIELLVLQTLSSWTSQ-KSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLY 215

Query: 121 CILDKGYNCFPTRLPGTPYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDE 180
             L++GYN  P  LPGT + K++ AR +L E L ++I +RRE    E  L+G LL  +D+
Sbjct: 216 QRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEGGLLGVLLGAKDQ 275

Query: 181 NGQSLSEDQIADNIIGVLFAAQDTTATALTWILKYLHDNLKLFEAVKAEQMEIYRRNGEG 240
               LS+ QIADNIIGV+FAA DTTA+ LTW+LKYLHD+  L + V  EQ  I ++  + 
Sbjct: 276 KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKE 335

Query: 241 KMPLSWAQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNI 300
              +SW   + MP T RV+ E+LR AS++SFTFREAV DVEY GYLIPKGWKV+PLFR I
Sbjct: 336 NRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRI 395

Query: 301 HHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRW 360
           HH+ E+FPDP  FDPSRFEVAP+P T+MPFGNGVHSCPG+ELAKLE+LILLHHL T FRW
Sbjct: 396 HHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRW 455

Query: 361 EVVGSQSGVEYGPFPMPVHGLPAR 384
           EV+G + G++YGPFP+P  GLP R
Sbjct: 456 EVIGDEEGIQYGPFPVPKKGLPIR 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887333.14.9e-21896.17abscisic acid 8'-hydroxylase 4 [Benincasa hispida][more]
XP_008454947.13.5e-21695.15PREDICTED: abscisic acid 8'-hydroxylase 4 [Cucumis melo][more]
KAA0031296.13.5e-21695.15abscisic acid 8'-hydroxylase 4 [Cucumis melo var. makuwa] >TYK06748.1 abscisic a... [more]
XP_004137112.16.0e-21695.41abscisic acid 8'-hydroxylase 4 [Cucumis sativus] >KAE8645865.1 hypothetical prot... [more]
XP_022927667.13.9e-20791.33abscisic acid 8'-hydroxylase 4-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LJK22.1e-15567.71Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 ... [more]
A9QNE71.5e-15368.29Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A... [more]
Q09J784.5e-14262.76Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Q0J1853.8e-14162.34Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70... [more]
A2Z2128.5e-14162.34Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Match NameE-valueIdentityDescription
A0A5A7SMJ81.7e-21695.15Abscisic acid 8'-hydroxylase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BZQ31.7e-21695.15abscisic acid 8'-hydroxylase 4 OS=Cucumis melo OX=3656 GN=LOC103495236 PE=3 SV=1[more]
A0A0A0K6K82.9e-21695.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073680 PE=3 SV=1[more]
A0A6J1EHU31.9e-20791.33abscisic acid 8'-hydroxylase 4-like OS=Cucurbita moschata OX=3662 GN=LOC11143448... [more]
A0A6J1JMR75.1e-20590.05abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111486079 ... [more]
Match NameE-valueIdentityDescription
AT3G19270.11.5e-15667.71cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT5G45340.17.2e-12757.48cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT4G19230.18.8e-12556.43cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT2G29090.15.3e-12256.77cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
AT2G29090.25.3e-12256.77cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 295..319
score: 27.21
coord: 255..273
score: 22.97
coord: 326..336
score: 47.18
coord: 188..205
score: 21.19
coord: 336..359
score: 30.32
IPR001128Cytochrome P450PRINTSPR00385P450coord: 199..216
score: 37.03
coord: 256..267
score: 33.62
coord: 327..336
score: 47.79
coord: 336..347
score: 39.37
IPR001128Cytochrome P450PFAMPF00067p450coord: 3..362
e-value: 2.2E-61
score: 208.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..386
e-value: 1.9E-99
score: 335.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 2..385
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 1..386
NoneNo IPR availablePANTHERPTHR24286:SF103CYTOCHROME P450 FAMILY ABA 8'-HYDROXYLASEcoord: 1..386
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 329..338

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
HG10018604HG10018604gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
HG10018604.1-cdsHG10018604.1-cds-Chr04:5729414..5729519CDS
HG10018604.1-cdsHG10018604.1-cds-Chr04:5730171..5730289CDS
HG10018604.1-cdsHG10018604.1-cds-Chr04:5730422..5730528CDS
HG10018604.1-cdsHG10018604.1-cds-Chr04:5730643..5730721CDS
HG10018604.1-cdsHG10018604.1-cds-Chr04:5731069..5731158CDS
HG10018604.1-cdsHG10018604.1-cds-Chr04:5731650..5731898CDS
HG10018604.1-cdsHG10018604.1-cds-Chr04:5732001..5732150CDS
HG10018604.1-cdsHG10018604.1-cds-Chr04:5732893..5733171CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
HG10018604.1HG10018604.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0010295 (+)-abscisic acid 8'-hydroxylase activity