Cucsat.G9905.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G9905.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	RING/FYVE/PHD zinc finger superfamily protein
Location	ctg1673: 1470881 .. 1474450 (+)
RNA-Seq Expression	Cucsat.G9905.T2
Synteny	Cucsat.G9905.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGATGGGGAAGAAAGGAAGCAAAGCAAGAGGTAGGGTTGAAAGCGGGGCCGGTCATTATGGCGCCAACAAATAAATTAGGAAGCGACTAAATTATATCCACCTGGGTCCTTATTCACAATTCATAACGAACATTTTACAATTCCCGTTATTAGATTCTTTATGGGAATAACTCCCGTTTTTGACGCTCAATCCGACGAACCCATCCCCGATTTCATAGCATTACCACACGCATCTTCTAATAACGGGAATTGTAAAATGTTCGATTTCATCCAAACCCACTTTCATTCGATTAAATCCCCTCCTTTTTCTTCCTCTTCTCTTCAACTCTCCTCTGGAATCCCTCCGGTCCGCTGCCTTCTCAGCGCGATCACTGGGGATTCTGGTTCTTCTCCCATTTCTACTACTACTACGCCTCTTGAGCTTTGTTTTTTTGCCCACTTTGGTGTTTCATTTTTGTTTGCGGAGCTATGAGCGATCATCTCGTTCTGTATGTGGACCGATTAGCTCGGCCGCCACCCCTTGATTCCATCCCCAACCCACCTGAGGCTGTTCCTCTCTTGCCGGTTGATGGGGATTTGGACAGCGACTCACCTTTGTCTTCTCCAACCTCTGCTTCCAGTGTTGAGGAGAGGGGAGAAGAAAATGAGGGTTCGGATGGAGAAGATGATTCTTTGATTCAGACAGCGGAGTGCCGCATTTGTCAGGATGAGGATGTAATTAGGAAGCTGGAAACTCCTTGTGCCTGTAGTGGCAGCCTTAAGGTATAAGTGATTTTTCAATTTAAGTATTGTAAAACTTTCTACTTTCTTTGTAAATGTTTCGGCTTTACGGGATTACATTAGTGATCATTAAGTAATTTGAAGCATAAATATATAGGGATTATTTAGCATCTTATTTGTTTTTGTAGCTCAAGTTGCAGTCATTGGCCGGCCATTGTGGTTGGCTAATTAAGTCGGTGTTTATGTCAAATTTACAAAAACTAGTTGTATATGCTTAAGTGAATGCCATAAATCTAAGAATGTTCGGTAGGAAAGTTTTATCGTAGGGTCAAATGTCTGTTTATGTTTATCTTGTTTTGTTTGTCTCAAATATATCCAATGAGACATTCGTTGGTTGTGTTGCATTACGATTTAGATGAGATGTTAACAACGATTGCTTTCTCAGTATGCTCATCGGAAGTGTATACAGCTTTGGTGCAATGAGAAAGGGGATATTATTTGTGAGATTTGTCATCAGGTATGTTCACTTTTTTTTATTGTACTTTGTTATTTTAAGTTCGTGGAATCATGTGTGCATTTGACATCAAAGATTCAAAGTTGTTCTAGAAGAGATGTTGTTGGACTTGCACCCCCTTTTGGGATATAGCAACGGTTTTTCTGCTGGTGTAGAGTGTTTGGGTTGAAAGAAATATTAGAATTTTTAGAAATGTCAATTGACCTTGGTTTGATATGCCCTATAATGTTAGATCTAAAACTTCGTTGTGGCTACTACTTCTCAGGCTTTGTGAAATTATTAGCTTGTTTGATTCTTTTGGACCAGAGTCCCTCTCTTTGGATGAACACCCTTTGTTGGGAGGTCGTTTTTGTATTGCCCTTTGTTTTTCCATAAGCTGGGTTCTTGTCAATATATCTATATATGAGTTCGTGAGAGCTCGTCTGACTTCTTAAGTGGACACGTATTATTCCATTCAATATTTTGTGTATGGGCCATGACACTATCTGGGGAAAGCATCTTGAAAGAAAAACATGTTTAACGTAATGGTATTTCCATTGGAAAATGAAGTCAAGAAATGGTATATGACCTTACTTGAACGGGATATGTTCTATAGGTTGTACATCTGGGTCCTTGAGTTCTAGGGAGACAAGAAATGGTACAGGCTGACTATTGAAAGATAAAACAATTTAAATAATGATGAGTGTAGACGCGTGTATATATATATATATATATATATGGAAACAATTCAGCTACATTGAATGCCATATTTTTTAGAGGTTTCAACTTACCTGAATTTATATGCTTGCATATGACGCACAGCCTTACCAACCTGATTATACAGCTCCGCCACCTCCCCCTCGCATTGAAGAAACATCTATTGACATCGGGTACCTTACATGCTTCAAATTTCCTTACTTTAGAAAGTTTTGAGATATTTTATGAAATCTTTTTGTTTATTTGAAAAATTGTGCTGTACTTCTTTTCTCGATATTTTGCATTTGAGATGTTAAGCTAAACATGTCCTATTCTTTGCTTTCTGCAGGGGAGGGTGGACCATCACTGGCACCCCTTTGAATTTACACGATCCTCGCTTTTTGGCTATTGCTGAGGCTGAGCATAATATATTGGAAGCCGAGTATGATGAATATGCTGCTTCAGATGCCAGTGGAGCTGCATTTTGTCGAGCAGCTGCTCTTATTGTAAGTTTGTTGTTCGATAATTGATACCACACGTAAAAAATCTCAGTAGTATAATCAAGAAACTTAGTATTGTCATACACATTTTATAAACTGATGTCACGATTAATTAGTGGGCTAATACCTCACATTTGAAGGCGTTTTCACACTTCTCTTTGAATTACTCTCATACAAAGTTGACATTGCAGTTTCATATTCTCATGGAAAATGTATGAACTAATGACCTGTTTGCTGATGTTCTTTTTATTCAATTGGGATAAAATGACATCATTTGTTTAATTTCAATATGGCAGCTCATGATCCTACTTTTCTTACGTCACGCACTTGAAGTCACAGATCCTGATGGTGACGATTACTTGTCTGCTTTCTTCTCTGTAAGTTAGAGTTCATTGTTTCAATCTGGAATGGACAACAATCTCCAGCAGTTTTCCTTTCTCACAGATCTTTGACATTCCTCATCTTCTGGACATTTTTTTAACCATACCATCCAGATTTTCTTGCTTCGAGCAGCTGGTTTTCTCTTGCCCTGTTACATTATGGCTTGTGCAGTCAACATCTTGCAACGTCGACAACGACGACAGGTGGGCATTACCTCCCACATTATCCTTAACTAATAATTCAAGGATAAATTTTTTTACCTAACGTTTCGTTTTCCTTTTATGTTTTGCCTAATTTGAGGAACGGGAGGCTGCAGCCTTGGGTGCAGCACAAGTTGCTTTTGTGCTACAATCAGGACGGCACAGGGGGCTGCAATTTGCAATAGCACCAGGACCTCAAATGACTCCCCACCAGGAGACTGTTTAACTTACATAGGCACAAAATGTATGACCTCTTCATTTTTTCCCCAATGGTTTTAGCACAAAAACGGAAAATCTAGATCTGCCTTGAAATCTGTCCATATTTGTAATCTGTCTTACATTCATTTTGGAAAGCCCGAGTGTAATTCTTCTCAAGAATGCTATAGCTTTTCATGATCTTCAATCTCCTGCCCCTTGAGTAATGTAGCAACGGAAAACTTAAAACCAAAATTTCAGTAGTTGAATGGTGTACACGAAACAGAGTTTGTATAAATTGTTTAAAGATCTTTGTTTCGAATCTTACATATAAAAGGAAGAACTATGTCTGGTTTGTCTTGTAAATTACCTTGTCAATTTTT

Coding sequence (CDS)

ATGAGCGATCATCTCGTTCTGTATGTGGACCGATTAGCTCGGCCGCCACCCCTTGATTCCATCCCCAACCCACCTGAGGCTGTTCCTCTCTTGCCGGTTGATGGGGATTTGGACAGCGACTCACCTTTGTCTTCTCCAACCTCTGCTTCCAGTGTTGAGGAGAGGGGAGAAGAAAATGAGGGTTCGGATGGAGAAGATGATTCTTTGATTCAGACAGCGGAGTGCCGCATTTGTCAGGATGAGGATGTAATTAGGAAGCTGGAAACTCCTTGTGCCTGTAGTGGCAGCCTTAAGTATGCTCATCGGAAGTGTATACAGCTTTGGTGCAATGAGAAAGGGGATATTATTTGTGAGATTTGTCATCAGCCTTACCAACCTGATTATACAGCTCCGCCACCTCCCCCTCGCATTGAAGAAACATCTATTGACATCGGGGGAGGGTGGACCATCACTGGCACCCCTTTGAATTTACACGATCCTCGCTTTTTGGCTATTGCTGAGGCTGAGCATAATATATTGGAAGCCGAGTATGATGAATATGCTGCTTCAGATGCCAGTGGAGCTGCATTTTGTCGAGCAGCTGCTCTTATTCTCATGATCCTACTTTTCTTACGTCACGCACTTGAAGTCACAGATCCTGATGGTGACGATTACTTGTCTGCTTTCTTCTCTGTAAGTTAG

Protein sequence

MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDSPLSSPTSASSVEERGEENEGSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEICHQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEYDEYAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSVS

Homology

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BLAST of Cucsat.G9905.T2 vs. ExPASy Swiss-Prot
Match: Q80TE3 (E3 ubiquitin-protein ligase MARCHF4 OS=Mus musculus OX=10090 GN=Marchf4 PE=2 SV=3)

HSP 1 Score: 60.5 bits (145), Expect = 2.9e-08
Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 0


Query: 22  PNPPEAVPLLPVDGDLDSDSPLSSP-----TSASSVE---ERGEENEGSDGEDDSLIQTA 81
           P PP   P LP  GD D D P + P     +SASS E   E+ E+        DS ++T 
Sbjct: 103 PLPPP--PPLPPSGDDDWDGPATGPPASLLSSASSDEFCKEKTEDCYSLGSSLDSGMRTP 162

Query: 82  ECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEICHQPY 125
            CRIC       +L +PC C GS+K  H+ C+  W +E+G   CE+C+  Y
Sbjct: 163 LCRICFQGPEQGELLSPCRCDGSVKCTHQPCLIKWISERGCWSCELCYYKY 211

[Close]

BLAST of Cucsat.G9905.T2 vs. ExPASy Swiss-Prot
Match: Q9P2E8 (E3 ubiquitin-protein ligase MARCHF4 OS=Homo sapiens OX=9606 GN=MARCHF4 PE=2 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 4.2e-07
Identity = 40/115 (34.78%), Postives = 58/115 (50.43%), Query Frame = 0


Query: 15  PPPLDSIPNPPEAVPLLPVD--GDLDSDSPLSSPTSASS---VEERGEENEGSDGEDDSL 74
           PPP   +P PP   P    D  G   ++ P S  +SASS    +E+ E+        DS 
Sbjct: 101 PPP---VPPPPPLPPSSVEDDWGGPATEPPASLLSSASSDDFCKEKTEDRYSLGSSLDSG 160

Query: 75  IQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEICHQPY 125
           ++T  CRIC       +L +PC C GS+K  H+ C+  W +E+G   CE+C+  Y
Sbjct: 161 MRTPLCRICFQGPEQGELLSPCRCDGSVKCTHQPCLIKWISERGCWSCELCYYKY 212

[Close]

BLAST of Cucsat.G9905.T2 vs. ExPASy Swiss-Prot
Match: Q86YJ5 (E3 ubiquitin-protein ligase MARCHF9 OS=Homo sapiens OX=9606 GN=MARCHF9 PE=1 SV=2)

HSP 1 Score: 56.2 bits (134), Expect = 5.5e-07
Identity = 36/104 (34.62%), Postives = 50/104 (48.08%), Query Frame = 0


Query: 22  PNPPEAVPLLPVDGDLDSDSPLSSPTSASSVEERGEENEGSDGEDDSLIQTAECRICQDE 81
           P PP A P LP  G LD+ S  SS                     DS ++T +CRIC   
Sbjct: 78  PLPPPAPP-LPPPGALDALSLSSSL--------------------DSGLRTPQCRICFQG 137

Query: 82  DVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEICHQPYQ 126
               +L +PC C GS++  H+ C+  W +E+G   CE+C+  YQ
Sbjct: 138 PEQGELLSPCRCDGSVRCTHQPCLIRWISERGSWSCELCYFKYQ 160

[Close]

BLAST of Cucsat.G9905.T2 vs. ExPASy Swiss-Prot
Match: Q3TZ87 (E3 ubiquitin-protein ligase MARCHF9 OS=Mus musculus OX=10090 GN=Marchf9 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.5e-07
Identity = 33/115 (28.70%), Postives = 48/115 (41.74%), Query Frame = 0


Query: 11  RLARPPPLDSIPNPPEAVPLLPVDGDLDSDSPLSSPTSASSVEERGEENEGSDGEDDSLI 70
           R   PPP    P PP A+  L +   LDS                              +
Sbjct: 75  RAGPPPPPAPPPPPPGALDALSLSSSLDSG-----------------------------L 134

Query: 71  QTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEICHQPYQ 126
           +T +CRIC       +L +PC C GS++  H+ C+  W +E+G   CE+C+  YQ
Sbjct: 135 RTPQCRICFQGPEQGELLSPCRCDGSVRCTHQPCLIRWISERGSWSCELCYFKYQ 160

[Close]

BLAST of Cucsat.G9905.T2 vs. ExPASy Swiss-Prot
Match: F4JKK0 (Probable E3 ubiquitin ligase SUD1 OS=Arabidopsis thaliana OX=3702 GN=SUD1 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 7.1e-07
Identity = 37/114 (32.46%), Postives = 53/114 (46.49%), Query Frame = 0


Query: 17  PLDSIPNPPEAVPLLPVDGDLDSDSPLSSPTSA-----SSVEERGEENEGSDGEDDSLIQ 76
           P DS+     A   +  +  + S S  SSP  A     S+++       GS   DD   +
Sbjct: 5   PADSLSISGAAASEVVSEPSVSSSSSSSSPNQASPNPFSNMDPAVSTATGSRYVDDDEDE 64

Query: 77  TAECRICQDE-DVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEICHQPY 125
              CRIC++  D    L  PCACSGS+K+ H+ C+  W N      CE+C  P+
Sbjct: 65  EDVCRICRNPGDADNPLRYPCACSGSIKFVHQDCLLQWLNHSNARQCEVCKHPF 118

[Close]

BLAST of Cucsat.G9905.T2 vs. NCBI nr
Match: XP_004135456.1 (uncharacterized protein LOC101205076 [Cucumis sativus] >KGN51915.1 hypothetical protein Csa_008417 [Cucumis sativus])

HSP 1 Score: 456 bits (1173), Expect = 8.99e-161
Identity = 224/225 (99.56%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDSPLSSPTSASSVEERGEENE 60
           MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDSPLSSPTSASSVEERGEENE
Sbjct: 1   MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDSPLSSPTSASSVEERGEENE 60

Query: 61  GSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEIC 120
           GSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEIC
Sbjct: 61  GSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEIC 120

Query: 121 HQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEYDEY 180
           HQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEYDEY
Sbjct: 121 HQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEYDEY 180

Query: 181 AASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSV 225
           AASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFS+
Sbjct: 181 AASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSI 225

[Close]

BLAST of Cucsat.G9905.T2 vs. NCBI nr
Match: XP_008446351.1 (PREDICTED: uncharacterized protein LOC103489118 [Cucumis melo] >KAA0034417.1 zinc finger family protein [Cucumis melo var. makuwa] >TYK17716.1 zinc finger family protein [Cucumis melo var. makuwa])

HSP 1 Score: 424 bits (1090), Expect = 4.11e-148
Identity = 210/226 (92.92%), Postives = 217/226 (96.02%), Query Frame = 0


Query: 1   MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDSP-LSSPTSASSVEERGEEN 60
           MSDHLVLYVDRL RPPP+DS+PNP EAVPLLPV GDL+SDS  LSS TSAS+VEERGEEN
Sbjct: 1   MSDHLVLYVDRLVRPPPVDSVPNPAEAVPLLPVGGDLESDSVGLSSSTSASNVEERGEEN 60

Query: 61  EGSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEI 120
           EG DGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEI
Sbjct: 61  EGLDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEI 120

Query: 121 CHQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEYDE 180
           CHQPYQP YTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPR LAIAEAEHN+LEAEYD+
Sbjct: 121 CHQPYQPGYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRLLAIAEAEHNLLEAEYDD 180

Query: 181 YAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSV 225
           YAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFS+
Sbjct: 181 YAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSI 226

[Close]

BLAST of Cucsat.G9905.T2 vs. NCBI nr
Match: XP_038891937.1 (uncharacterized protein LOC120081275 [Benincasa hispida])

HSP 1 Score: 405 bits (1042), Expect = 8.38e-141
Identity = 202/226 (89.38%), Postives = 209/226 (92.48%), Query Frame = 0


Query: 1   MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDSP-LSSPTSASSVEERGEEN 60
           MSDHLVLYVDR+ RPPP+DS+PNP E VPLL V GDL SDS  LSS TS S+ +ER  EN
Sbjct: 1   MSDHLVLYVDRIIRPPPVDSVPNPTEVVPLLRVGGDLASDSVGLSSSTSTSNFDERAHEN 60

Query: 61  EGSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEI 120
           EG DGE DSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEI
Sbjct: 61  EGLDGEADSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEI 120

Query: 121 CHQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEYDE 180
           CHQPYQP YTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPR LAIAEAEHN+LEAEYDE
Sbjct: 121 CHQPYQPGYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRLLAIAEAEHNLLEAEYDE 180

Query: 181 YAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSV 225
           YAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFS+
Sbjct: 181 YAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSI 226

[Close]

BLAST of Cucsat.G9905.T2 vs. NCBI nr
Match: XP_023552876.1 (uncharacterized protein LOC111810398 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 395 bits (1016), Expect = 7.36e-137
Identity = 196/228 (85.96%), Postives = 208/228 (91.23%), Query Frame = 0


Query: 1   MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDS---PLSSPTSASSVEERGE 60
           MSDHLVLYVDRL RP P+DS+PNP E VP LPV GDL+ DS   P S  TS S+V+E+ +
Sbjct: 1   MSDHLVLYVDRLIRPVPVDSVPNPTEIVPFLPVGGDLEGDSLGFPCS--TSTSNVDEKRK 60

Query: 61  ENEGSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIIC 120
           E+E  DGEDDSLIQTAECRICQD+D+IRKLETPCACSGSLKYAHRKCIQLWCNEKGDIIC
Sbjct: 61  ESEDLDGEDDSLIQTAECRICQDDDIIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIIC 120

Query: 121 EICHQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEY 180
           EICHQPYQP YTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPR LAIAEAEHN+LEAEY
Sbjct: 121 EICHQPYQPGYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRLLAIAEAEHNLLEAEY 180

Query: 181 DEYAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSV 225
           DEYAASDASGAAFCRAAA ILMILLFLRHALEVTDPDGDDYLSAFFS+
Sbjct: 181 DEYAASDASGAAFCRAAAFILMILLFLRHALEVTDPDGDDYLSAFFSI 226

[Close]

BLAST of Cucsat.G9905.T2 vs. NCBI nr
Match: KAG7032215.1 (E3 ubiquitin-protein ligase MARCH4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 396 bits (1017), Expect = 1.70e-136
Identity = 198/229 (86.46%), Postives = 208/229 (90.83%), Query Frame = 0


Query: 1   MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDS---PLSSPTSASSVEERGE 60
           MSDHLVLYVDRL RP P+DS+PNP E VP LPV GDL+ DS   P S  TS S+V+E+  
Sbjct: 1   MSDHLVLYVDRLIRPVPVDSVPNPTEIVPFLPVGGDLEGDSLGFPCS--TSTSNVDEKRI 60

Query: 61  ENEGSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIIC 120
           E+E  DGEDDSLIQTAECRICQD+D+IRKLETPCACSGSLKYAHRKCIQLWCNEKGDIIC
Sbjct: 61  ESEDLDGEDDSLIQTAECRICQDDDIIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIIC 120

Query: 121 EICHQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEY 180
           EICHQPYQP YTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPR LAIAEAEHN+LEAEY
Sbjct: 121 EICHQPYQPGYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRLLAIAEAEHNLLEAEY 180

Query: 181 DEYAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSVS 226
           DEYAASDASGAAFCRAAA ILMILLFLRHALEVTDPDGDDYLSAFFSVS
Sbjct: 181 DEYAASDASGAAFCRAAAFILMILLFLRHALEVTDPDGDDYLSAFFSVS 227

[Close]

BLAST of Cucsat.G9905.T2 vs. ExPASy TrEMBL
Match: A0A0A0KQL3 (RING-CH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G605090 PE=4 SV=1)

HSP 1 Score: 456 bits (1173), Expect = 4.36e-161
Identity = 224/225 (99.56%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDSPLSSPTSASSVEERGEENE 60
           MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDSPLSSPTSASSVEERGEENE
Sbjct: 1   MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDSPLSSPTSASSVEERGEENE 60

Query: 61  GSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEIC 120
           GSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEIC
Sbjct: 61  GSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEIC 120

Query: 121 HQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEYDEY 180
           HQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEYDEY
Sbjct: 121 HQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEYDEY 180

Query: 181 AASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSV 225
           AASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFS+
Sbjct: 181 AASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSI 225

[Close]

BLAST of Cucsat.G9905.T2 vs. ExPASy TrEMBL
Match: A0A5A7SYR0 (Zinc finger family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00440 PE=4 SV=1)

HSP 1 Score: 424 bits (1090), Expect = 1.99e-148
Identity = 210/226 (92.92%), Postives = 217/226 (96.02%), Query Frame = 0


Query: 1   MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDSP-LSSPTSASSVEERGEEN 60
           MSDHLVLYVDRL RPPP+DS+PNP EAVPLLPV GDL+SDS  LSS TSAS+VEERGEEN
Sbjct: 1   MSDHLVLYVDRLVRPPPVDSVPNPAEAVPLLPVGGDLESDSVGLSSSTSASNVEERGEEN 60

Query: 61  EGSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEI 120
           EG DGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEI
Sbjct: 61  EGLDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEI 120

Query: 121 CHQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEYDE 180
           CHQPYQP YTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPR LAIAEAEHN+LEAEYD+
Sbjct: 121 CHQPYQPGYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRLLAIAEAEHNLLEAEYDD 180

Query: 181 YAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSV 225
           YAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFS+
Sbjct: 181 YAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSI 226

[Close]

BLAST of Cucsat.G9905.T2 vs. ExPASy TrEMBL
Match: A0A1S3BFI9 (uncharacterized protein LOC103489118 OS=Cucumis melo OX=3656 GN=LOC103489118 PE=4 SV=1)

HSP 1 Score: 424 bits (1090), Expect = 1.99e-148
Identity = 210/226 (92.92%), Postives = 217/226 (96.02%), Query Frame = 0


Query: 1   MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDSP-LSSPTSASSVEERGEEN 60
           MSDHLVLYVDRL RPPP+DS+PNP EAVPLLPV GDL+SDS  LSS TSAS+VEERGEEN
Sbjct: 1   MSDHLVLYVDRLVRPPPVDSVPNPAEAVPLLPVGGDLESDSVGLSSSTSASNVEERGEEN 60

Query: 61  EGSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEI 120
           EG DGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEI
Sbjct: 61  EGLDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIICEI 120

Query: 121 CHQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEYDE 180
           CHQPYQP YTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPR LAIAEAEHN+LEAEYD+
Sbjct: 121 CHQPYQPGYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRLLAIAEAEHNLLEAEYDD 180

Query: 181 YAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSV 225
           YAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFS+
Sbjct: 181 YAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSI 226

[Close]

BLAST of Cucsat.G9905.T2 vs. ExPASy TrEMBL
Match: A0A6J1GZ22 (uncharacterized protein LOC111458795 OS=Cucurbita moschata OX=3662 GN=LOC111458795 PE=4 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 1.45e-136
Identity = 196/228 (85.96%), Postives = 207/228 (90.79%), Query Frame = 0


Query: 1   MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDS---PLSSPTSASSVEERGE 60
           MSDHLVLYVDRL RP P+DS+PNP E VP LPV GDL+ DS   P S  TS S+V+E+  
Sbjct: 1   MSDHLVLYVDRLIRPVPVDSVPNPTEIVPFLPVGGDLEGDSLGFPCS--TSTSNVDEKRI 60

Query: 61  ENEGSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIIC 120
           E+E  DGEDDSLIQTAECRICQD+D+IRKLETPCACSGSLKYAHRKCIQLWCNEKGDIIC
Sbjct: 61  ESEDLDGEDDSLIQTAECRICQDDDIIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIIC 120

Query: 121 EICHQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEY 180
           EICHQPYQP YTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPR LAIAEAEHN+LEAEY
Sbjct: 121 EICHQPYQPGYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRLLAIAEAEHNLLEAEY 180

Query: 181 DEYAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSV 225
           DEYAASDASGAAFCRAAA ILMILLFLRHALEVTDPDGDDYLSAFFS+
Sbjct: 181 DEYAASDASGAAFCRAAAFILMILLFLRHALEVTDPDGDDYLSAFFSI 226

[Close]

BLAST of Cucsat.G9905.T2 vs. ExPASy TrEMBL
Match: A0A6J1JZ00 (uncharacterized protein LOC111489151 OS=Cucurbita maxima OX=3661 GN=LOC111489151 PE=4 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 1.45e-136
Identity = 194/228 (85.09%), Postives = 208/228 (91.23%), Query Frame = 0


Query: 1   MSDHLVLYVDRLARPPPLDSIPNPPEAVPLLPVDGDLDSDS---PLSSPTSASSVEERGE 60
           MSDHLVLYVDRL RP P++S+PNP E VP LP+ GDL+ DS   P S  TS S+V+E+ +
Sbjct: 1   MSDHLVLYVDRLIRPVPVESVPNPTEVVPFLPIGGDLEGDSLGFPCS--TSTSNVDEKRK 60

Query: 61  ENEGSDGEDDSLIQTAECRICQDEDVIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIIC 120
           E+E  DGEDDSLIQTAECRICQD+D+IRKLETPCACSGSLKYAHRKCIQLWCNEKGDIIC
Sbjct: 61  ESEDLDGEDDSLIQTAECRICQDDDIIRKLETPCACSGSLKYAHRKCIQLWCNEKGDIIC 120

Query: 121 EICHQPYQPDYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRFLAIAEAEHNILEAEY 180
           EICHQPYQP YTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPR LAIAEAEHN+LEAEY
Sbjct: 121 EICHQPYQPGYTAPPPPPRIEETSIDIGGGWTITGTPLNLHDPRLLAIAEAEHNLLEAEY 180

Query: 181 DEYAASDASGAAFCRAAALILMILLFLRHALEVTDPDGDDYLSAFFSV 225
           DEYAASDASGAAFCRAAA ILMILLFLRHALEVTDPDGDDYLSAFFS+
Sbjct: 181 DEYAASDASGAAFCRAAAFILMILLFLRHALEVTDPDGDDYLSAFFSI 226

The following BLAST results are available for this feature:

BLAST of Cucsat.G9905.T2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q80TE3	2.9e-08	37.84	E3 ubiquitin-protein ligase MARCHF4 OS=Mus musculus OX=10090 GN=Marchf4 PE=2 SV=...	[more]
Q9P2E8	4.2e-07	34.78	E3 ubiquitin-protein ligase MARCHF4 OS=Homo sapiens OX=9606 GN=MARCHF4 PE=2 SV=2	[more]
Q86YJ5	5.5e-07	34.62	E3 ubiquitin-protein ligase MARCHF9 OS=Homo sapiens OX=9606 GN=MARCHF9 PE=1 SV=2	[more]
Q3TZ87	5.5e-07	28.70	E3 ubiquitin-protein ligase MARCHF9 OS=Mus musculus OX=10090 GN=Marchf9 PE=2 SV=...	[more]
F4JKK0	7.1e-07	32.46	Probable E3 ubiquitin ligase SUD1 OS=Arabidopsis thaliana OX=3702 GN=SUD1 PE=1 S...	[more]

BLAST of Cucsat.G9905.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135456.1	8.99e-161	99.56	uncharacterized protein LOC101205076 [Cucumis sativus] >KGN51915.1 hypothetical ...	[more]
XP_008446351.1	4.11e-148	92.92	PREDICTED: uncharacterized protein LOC103489118 [Cucumis melo] >KAA0034417.1 zin...	[more]
XP_038891937.1	8.38e-141	89.38	uncharacterized protein LOC120081275 [Benincasa hispida]	[more]
XP_023552876.1	7.36e-137	85.96	uncharacterized protein LOC111810398 [Cucurbita pepo subsp. pepo]	[more]
KAG7032215.1	1.70e-136	86.46	E3 ubiquitin-protein ligase MARCH4, partial [Cucurbita argyrosperma subsp. argyr...	[more]

BLAST of Cucsat.G9905.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KQL3	4.36e-161	99.56	RING-CH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G60509...	[more]
A0A5A7SYR0	1.99e-148	92.92	Zinc finger family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3BFI9	1.99e-148	92.92	uncharacterized protein LOC103489118 OS=Cucumis melo OX=3656 GN=LOC103489118 PE=...	[more]
A0A6J1GZ22	1.45e-136	85.96	uncharacterized protein LOC111458795 OS=Cucurbita moschata OX=3662 GN=LOC1114587...	[more]
A0A6J1JZ00	1.45e-136	85.09	uncharacterized protein LOC111489151 OS=Cucurbita maxima OX=3661 GN=LOC111489151...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 163..183
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 14..68
None	No IPR available	PANTHER	PTHR23012:SF181	E3 UBIQUITIN-PROTEIN LIGASE MARCH-RELATED	coord: 1..225
None	No IPR available	CDD	cd16495	RING_CH-C4HC3_MARCH	coord: 75..121 e-value: 2.81951E-18 score: 73.5156
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 72..125
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 74..121 e-value: 1.5E-18 score: 77.6
IPR011016	Zinc finger, RING-CH-type	PFAM	PF12906	RINGv	coord: 75..120 e-value: 5.1E-12 score: 45.7
IPR011016	Zinc finger, RING-CH-type	PROSITE	PS51292	ZF_RING_CH	coord: 67..127 score: 22.463652
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 59..131 e-value: 7.0E-19 score: 69.2
IPR022143	Protein of unknown function DUF3675	PFAM	PF12428	DUF3675	coord: 126..225 e-value: 1.2E-30 score: 106.5
IPR033275	E3 ubiquitin-protein ligase MARCH-like	PANTHER	PTHR23012	RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAINING	coord: 1..225

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G9905	Cucsat.G9905	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9905.T2.E1	Cucsat.G9905.T2.E1	exon
Cucsat.G9905.T2.E2	Cucsat.G9905.T2.E2	exon
Cucsat.G9905.T2.E3	Cucsat.G9905.T2.E3	exon
Cucsat.G9905.T2.E4	Cucsat.G9905.T2.E4	exon
Cucsat.G9905.T2.E5	Cucsat.G9905.T2.E5	exon
Cucsat.G9905.T2.E6	Cucsat.G9905.T2.E6	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9905.T2.C1	Cucsat.G9905.T2.C1	CDS
Cucsat.G9905.T2.C2	Cucsat.G9905.T2.C2	CDS
Cucsat.G9905.T2.C3	Cucsat.G9905.T2.C3	CDS
Cucsat.G9905.T2.C4	Cucsat.G9905.T2.C4	CDS
Cucsat.G9905.T2.C5	Cucsat.G9905.T2.C5	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9905.T2	Cucsat.G9905.T2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity