Cucsat.G9542.T32 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G9542.T32
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Pectin lyase-like superfamily protein
Location	ctg1673: 2193832 .. 2195432 (-)
RNA-Seq Expression	Cucsat.G9542.T32
Synteny	Cucsat.G9542.T32

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGTCTCAGTTTTTGCATGTGTAATACATTCTGATTTAAGGGATTTCTTGCAGGCATTAACCATTGACTCAAGTTCAAATATAAGAGTAAAAGGCCTTACCATTCAAAACAGCCAACAGATGCATTTCACCATTGCACGGTCTGAAACTGTTAGAATTACGGGGGTAAGAGTGTCAGCTCCAGGAGATAGCCCCAATACTGACGGGATTCATATCACTCAATCAACTAATGTTGTAGTTCAAAACTGCAAGATTTCAACAGGTTTTCATTCTGATCTTCACTTGTTACCATCTAAACTGGCTGGAAAGAGTATTAATTGATAATGGTTTTACAATTTATATGCAGGTGATGACTGCATCTCCATTGTTAATGCTAGCTCTGGCATCAAAATGAAGGGAATATCATGCGGACCAGGACATGGGATCAGGTATTTGCAAATGTTTTTTGTTTCACTCGACGTTGTTTGATTTATTTCAAGCACCAAAACCAGATGAATGCCTTAGAATTTCTCTCTTTGTTTGTTTGTTGCGGAATGGTGTTCCTCAGCATTGGAAGTTTAGGGAAAGACAACTCCACTGGCATTGTGACAAAGGTGGTGTTAGACACAGCCTACCTCAGAGAGACAACAAATGGTGTTAGAATCAAGACTTGGCAGGTTAGAATCACAAATTTAACCACCATTTTCATACTTAGTTTACATGGTTGATTACAACCAAGCACAAAGGGAAACTTTTTCTATTCATGTGGTACAGGGAGGTTCTGGATATGTTCGTGCCGTACGCTTCGAAAATGTGAGAATGGAAGATGTTGAGAACCCCATTATCATCGATCAGTTCTACTGTGACTCTCCTACTACTTGTGAAAGTCAGGTATTATCCTCCCTCGAAATCCAGAATTTGGCTTCTAATCAAACAGTAACATTAAGTATAAAATCATACAGTAGGACAGTTTGTTCATTATTCATCATCAAGTTCAATGCCTTTTGTTTCTGTCAGACATCAGCCGTGAAAATAAGCCAAATCATGTACCGAAACATAAGTGGAACCACAACGAGCAAGAACGCAATGAAATTCGCCTGTAGCGACAGTGTTCCATGCAGCAACATAATTCTAAGCAATGTCAACTTGGAGAAAACAGATGGAACAGTGGAGACCTATTGCCACGCTGCTCAAGGCTTTGGCTATGGTGTTGTGCACCCATCGGCAGACTGCTTAACTTCCAGCGACAATAACTTTGCATCTTCAGATCAAACGCAAGTTCTGGAGCTGGAGACAGTGCAACTCTCTACCGAACAGCACATTGTTCATACTGAACTCTGAGCTTATTGTCCTGTCTATCTAAACTAAACTACTAAAGGGATCATATTTTGGTAGAGCTTGGTTTTCAGGTGATCACCAACTCAGGAAATATTTGTAGCATATATTTACATAGCATACACAATTATTCCTACCCTTTTAACATCTAAATAGAAATATAGACTATGAAGTAAATGATTGTACTATCGCTAGAAGAAAATATACATATTTTGAGTTTATAGTAAAGTTTTTTAAAACCCAAGTTTCATATGGCATATGATTGATGGCATTCTAGTTCTAACTATA

Coding sequence (CDS)

ATGCGGACCAGGACATGGGATCAGGTATTTGCAAATGTTTTTTGTTTCACTCGACGTTGTTTGATTTATTTCAAGCACCAAAACCAGATGAATGCCTTAGAATTTCTCTCTTTGTTTGTTTGTTGCGGAATGGTGTTCCTCAGCATTGGAAGTTTAGGGAAAGACAACTCCACTGGCATTGTGACAAAGGTGGTGTTAGACACAGCCTACCTCAGAGAGACAACAAATGGTGTTAGAATCAAGACTTGGCAGGGAGGTTCTGGATATGTTCGTGCCGTACGCTTCGAAAATGTGAGAATGGAAGATGTTGAGAACCCCATTATCATCGATCAGTTCTACTGTGACTCTCCTACTACTTGTGAAAGTCAGACATCAGCCGTGAAAATAAGCCAAATCATGTACCGAAACATAAGTGGAACCACAACGAGCAAGAACGCAATGAAATTCGCCTGTAGCGACAGTGTTCCATGCAGCAACATAATTCTAAGCAATGTCAACTTGGAGAAAACAGATGGAACAGTGGAGACCTATTGCCACGCTGCTCAAGGCTTTGGCTATGGTGTTGTGCACCCATCGGCAGACTGCTTAACTTCCAGCGACAATAACTTTGCATCTTCAGATCAAACGCAAGTTCTGGAGCTGGAGACAGTGCAACTCTCTACCGAACAGCACATTGTTCATACTGAACTCTGA

Protein sequence

MRTRTWDQVFANVFCFTRRCLIYFKHQNQMNALEFLSLFVCCGMVFLSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIVHTEL

Homology

[Close]

BLAST of Cucsat.G9542.T32 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.0e-37
Identity = 85/186 (45.70%), Postives = 118/186 (63.44%), Query Frame = 0


Query: 20  CLIYFKHQNQMNALEFLSLFVCCGMVFLSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVR 79
           C+   K+  Q++    +S  +C     +SIGSLGK  S   V  + +DTA + +T NGVR
Sbjct: 251 CISIVKNSTQIS----ISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVR 310

Query: 80  IKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCESQTSAVKISQIMYRNISG 139
           IKTWQGGSG V  + F N++M +V NPIIIDQ+YCDS   C +QTSA+ I  I + ++ G
Sbjct: 311 IKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRG 370

Query: 140 TTTSKNAMKFACSDSVPCSNIILSNVNLEKT--DGTVETYCHAAQGFGYGVVHPSADCLT 199
           T+ SK A+K +CSDS PC NI+L +++LE +  DG  E++C  A G   G V+P   CL 
Sbjct: 371 TSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYP-PPCL- 430

Query: 200 SSDNNF 204
           S D +F
Sbjct: 431 SDDTSF 430

[Close]

BLAST of Cucsat.G9542.T32 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.6e-33
Identity = 77/151 (50.99%), Postives = 100/151 (66.23%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLGK NS   V+ V LD A   +T NG+RIKTWQGGSG    + +ENV M + ENP
Sbjct: 281 MSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENP 340

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           I+I+QFYC S   C++Q SAVKI  + ++NI GT+ +  A++  CSDSVPCSNI LSNV 
Sbjct: 341 ILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSATTAAIQLMCSDSVPCSNIKLSNVF 400

Query: 167 LEKTDGTVETYCHA-AQGFGYGVVHPSADCL 197
           L+ T G V T  +  A G+    ++PS   L
Sbjct: 401 LKLTSGKVATCVNKNANGYYTNPLNPSCKSL 431

[Close]

BLAST of Cucsat.G9542.T32 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 8.5e-32
Identity = 68/130 (52.31%), Postives = 88/130 (67.69%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLG  NS   V+ V++D   L +TTNG+RIKTWQGGSG  + ++F+N+ M +V NP
Sbjct: 303 ISIGSLGDRNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNP 362

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           IIIDQ+YCDS   C  Q SAVK+S + Y NI GT+ S+ A+KF CS S PC   I+ N+N
Sbjct: 363 IIIDQYYCDSKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNIN 422

Query: 167 LEKTDGTVET 177
           L    G   T
Sbjct: 423 LVGNGGKETT 432

[Close]

BLAST of Cucsat.G9542.T32 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.5e-31
Identity = 72/146 (49.32%), Postives = 94/146 (64.38%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLG  NS   V+ VV++ A L  TTNGVRIKTWQGGSG    ++F+NV M +VENP
Sbjct: 308 ISIGSLGYGNSEAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENP 367

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           IIIDQ YCD    C+ Q+SAV++  ++Y+NI GT  S  A+ F CS   PC  I+L +V+
Sbjct: 368 IIIDQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVD 427

Query: 167 LEKTDG-TVETYCHAAQGFGYGVVHP 192
           LE   G   +  C+  +    GVV P
Sbjct: 428 LEIEGGAAAKALCNNVELSETGVVSP 453

[Close]

BLAST of Cucsat.G9542.T32 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 3.2e-31
Identity = 71/147 (48.30%), Postives = 100/147 (68.03%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLG+DNS   V+ V ++ A   +T NG+RIKTWQGGSG    + +ENV M + ENP
Sbjct: 280 ISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENP 339

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           I+I+QFYC S + C++Q SAV+I  + Y+NI GT+ +  A++  CSDSVPC+ I LSNV+
Sbjct: 340 ILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVS 399

Query: 167 LEKTDGTVETYCHA-AQGFGYGVVHPS 193
           L+ T G   +     A+GF  G + P+
Sbjct: 400 LKLTSGKPASCVDKNARGFYSGRLIPT 426

[Close]

BLAST of Cucsat.G9542.T32 vs. NCBI nr
Match: XP_011655619.1 (probable polygalacturonase At1g80170 isoform X1 [Cucumis sativus])

HSP 1 Score: 367 bits (941), Expect = 7.29e-123
Identity = 183/184 (99.46%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP
Sbjct: 286 ISIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 345

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN
Sbjct: 346 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 405

Query: 167 LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV 226
           LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV
Sbjct: 406 LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV 465

Query: 227 HTEL 230
           HTEL
Sbjct: 466 HTEL 469

[Close]

BLAST of Cucsat.G9542.T32 vs. NCBI nr
Match: XP_008446196.1 (PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo])

HSP 1 Score: 357 bits (916), Expect = 1.40e-118
Identity = 176/184 (95.65%), Postives = 182/184 (98.91%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLGKDNSTGIVTKVVLDTA+LRETTNGVRIKTWQGGSGYVR+VRFENVRMEDVENP
Sbjct: 323 ISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENP 382

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           IIIDQFYCDSPTTCE+QTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN
Sbjct: 383 IIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 442

Query: 167 LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV 226
           LEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSDNNFA+ DQTQVLELETVQL TEQHIV
Sbjct: 443 LEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAALDQTQVLELETVQLPTEQHIV 502

Query: 227 HTEL 230
           HTEL
Sbjct: 503 HTEL 506

[Close]

BLAST of Cucsat.G9542.T32 vs. NCBI nr
Match: TYK15614.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 359 bits (922), Expect = 2.15e-118
Identity = 177/184 (96.20%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLGKDNSTGIVTKVVLDTA+LRETTNGVRIKTWQGGSGYVR+VRFENVRMEDVENP
Sbjct: 408 ISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENP 467

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           IIIDQFYCDSPTTCE+QTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN
Sbjct: 468 IIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 527

Query: 167 LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV 226
           LEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSDNNFA+SDQTQVLELETVQL TEQHIV
Sbjct: 528 LEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAASDQTQVLELETVQLPTEQHIV 587

Query: 227 HTEL 230
           HTEL
Sbjct: 588 HTEL 591

[Close]

BLAST of Cucsat.G9542.T32 vs. NCBI nr
Match: KAA0034306.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 359 bits (922), Expect = 2.21e-118
Identity = 177/184 (96.20%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLGKDNSTGIVTKVVLDTA+LRETTNGVRIKTWQGGSGYVR+VRFENVRMEDVENP
Sbjct: 409 ISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENP 468

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           IIIDQFYCDSPTTCE+QTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN
Sbjct: 469 IIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 528

Query: 167 LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV 226
           LEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSDNNFA+SDQTQVLELETVQL TEQHIV
Sbjct: 529 LEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAASDQTQVLELETVQLPTEQHIV 588

Query: 227 HTEL 230
           HTEL
Sbjct: 589 HTEL 592

[Close]

BLAST of Cucsat.G9542.T32 vs. NCBI nr
Match: ADN33827.1 (polygalacturonase [Cucumis melo subsp. melo])

HSP 1 Score: 357 bits (916), Expect = 2.96e-117
Identity = 176/184 (95.65%), Postives = 182/184 (98.91%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLGKDNSTGIVTKVVLDTA+LRETTNGVRIKTWQGGSGYVR+VRFENVRMEDVENP
Sbjct: 427 ISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENP 486

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           IIIDQFYCDSPTTCE+QTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN
Sbjct: 487 IIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 546

Query: 167 LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV 226
           LEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSDNNFA+ DQTQVLELETVQL TEQHIV
Sbjct: 547 LEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAALDQTQVLELETVQLPTEQHIV 606

Query: 227 HTEL 230
           HTEL
Sbjct: 607 HTEL 610

[Close]

BLAST of Cucsat.G9542.T32 vs. ExPASy TrEMBL
Match: A0A0A0KQ65 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600960 PE=3 SV=1)

HSP 1 Score: 367 bits (941), Expect = 3.53e-123
Identity = 183/184 (99.46%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP
Sbjct: 286 ISIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 345

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN
Sbjct: 346 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 405

Query: 167 LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV 226
           LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV
Sbjct: 406 LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV 465

Query: 227 HTEL 230
           HTEL
Sbjct: 466 HTEL 469

[Close]

BLAST of Cucsat.G9542.T32 vs. ExPASy TrEMBL
Match: A0A1S3BF58 (probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103489000 PE=3 SV=1)

HSP 1 Score: 357 bits (916), Expect = 6.75e-119
Identity = 176/184 (95.65%), Postives = 182/184 (98.91%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLGKDNSTGIVTKVVLDTA+LRETTNGVRIKTWQGGSGYVR+VRFENVRMEDVENP
Sbjct: 323 ISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENP 382

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           IIIDQFYCDSPTTCE+QTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN
Sbjct: 383 IIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 442

Query: 167 LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV 226
           LEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSDNNFA+ DQTQVLELETVQL TEQHIV
Sbjct: 443 LEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAALDQTQVLELETVQLPTEQHIV 502

Query: 227 HTEL 230
           HTEL
Sbjct: 503 HTEL 506

[Close]

BLAST of Cucsat.G9542.T32 vs. ExPASy TrEMBL
Match: A0A5D3CXE4 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001110 PE=3 SV=1)

HSP 1 Score: 359 bits (922), Expect = 1.04e-118
Identity = 177/184 (96.20%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLGKDNSTGIVTKVVLDTA+LRETTNGVRIKTWQGGSGYVR+VRFENVRMEDVENP
Sbjct: 408 ISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENP 467

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           IIIDQFYCDSPTTCE+QTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN
Sbjct: 468 IIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 527

Query: 167 LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV 226
           LEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSDNNFA+SDQTQVLELETVQL TEQHIV
Sbjct: 528 LEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAASDQTQVLELETVQLPTEQHIV 587

Query: 227 HTEL 230
           HTEL
Sbjct: 588 HTEL 591

[Close]

BLAST of Cucsat.G9542.T32 vs. ExPASy TrEMBL
Match: A0A5A7SWQ6 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003060 PE=3 SV=1)

HSP 1 Score: 359 bits (922), Expect = 1.07e-118
Identity = 177/184 (96.20%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLGKDNSTGIVTKVVLDTA+LRETTNGVRIKTWQGGSGYVR+VRFENVRMEDVENP
Sbjct: 409 ISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENP 468

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           IIIDQFYCDSPTTCE+QTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN
Sbjct: 469 IIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 528

Query: 167 LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV 226
           LEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSDNNFA+SDQTQVLELETVQL TEQHIV
Sbjct: 529 LEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAASDQTQVLELETVQLPTEQHIV 588

Query: 227 HTEL 230
           HTEL
Sbjct: 589 HTEL 592

[Close]

BLAST of Cucsat.G9542.T32 vs. ExPASy TrEMBL
Match: E5GBI5 (Polygalacturonase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 357 bits (916), Expect = 1.43e-117
Identity = 176/184 (95.65%), Postives = 182/184 (98.91%), Query Frame = 0


Query: 47  LSIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENP 106
           +SIGSLGKDNSTGIVTKVVLDTA+LRETTNGVRIKTWQGGSGYVR+VRFENVRMEDVENP
Sbjct: 427 ISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENP 486

Query: 107 IIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 166
           IIIDQFYCDSPTTCE+QTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN
Sbjct: 487 IIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVN 546

Query: 167 LEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIV 226
           LEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSDNNFA+ DQTQVLELETVQL TEQHIV
Sbjct: 547 LEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAALDQTQVLELETVQLPTEQHIV 606

Query: 227 HTEL 230
           HTEL
Sbjct: 607 HTEL 610

The following BLAST results are available for this feature:

BLAST of Cucsat.G9542.T32 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AJ5	1.0e-37	45.70	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
Q9FY19	1.6e-33	50.99	Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1	[more]
Q02096	8.5e-32	52.31	Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1	[more]
P35336	1.5e-31	49.32	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1	[more]
Q7M1E7	3.2e-31	48.30	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]

BLAST of Cucsat.G9542.T32 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011655619.1	7.29e-123	99.46	probable polygalacturonase At1g80170 isoform X1 [Cucumis sativus]	[more]
XP_008446196.1	1.40e-118	95.65	PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo]	[more]
TYK15614.1	2.15e-118	96.20	polygalacturonase [Cucumis melo var. makuwa]	[more]
KAA0034306.1	2.21e-118	96.20	polygalacturonase [Cucumis melo var. makuwa]	[more]
ADN33827.1	2.96e-117	95.65	polygalacturonase [Cucumis melo subsp. melo]	[more]

BLAST of Cucsat.G9542.T32 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KQ65	3.53e-123	99.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600960 PE=3 SV=1	[more]
A0A1S3BF58	6.75e-119	95.65	probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103489000 PE=...	[more]
A0A5D3CXE4	1.04e-118	96.20	Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001...	[more]
A0A5A7SWQ6	1.07e-118	96.20	Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003...	[more]
E5GBI5	1.43e-117	95.65	Polygalacturonase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 33..189 e-value: 8.0E-44 score: 152.1
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 47..178 e-value: 1.2E-37 score: 129.7
None	No IPR available	PANTHER	PTHR31375:SF205	BNAA08G20470D PROTEIN	coord: 45..208
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 45..208
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 47..198

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G9542	Cucsat.G9542	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9542.T32.E1	Cucsat.G9542.T32.E1	exon
Cucsat.G9542.T32.E2	Cucsat.G9542.T32.E2	exon
Cucsat.G9542.T32.E3	Cucsat.G9542.T32.E3	exon
Cucsat.G9542.T32.E4	Cucsat.G9542.T32.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9542.T32.C3	Cucsat.G9542.T32.C3	CDS
Cucsat.G9542.T32.C2	Cucsat.G9542.T32.C2	CDS
Cucsat.G9542.T32.C1	Cucsat.G9542.T32.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9542.T32	Cucsat.G9542.T32-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004650	polygalacturonase activity
molecular_function	GO:0008234	cysteine-type peptidase activity