Cucsat.G9343.T12 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G9343.T12
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Diacylglycerol O-acyltransferase
Location	ctg1658: 1769664 .. 1772738 (-)
RNA-Seq Expression	Cucsat.G9343.T12
Synteny	Cucsat.G9343.T12

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCAAAACTTATTTATAAATATAATAAAAAGAATTTGTCATAAACATCTATCAATTCGTATCATTGTAATCTATCACTAATCAATAATTAAAATTTATTATATTTATAAATATTTTTAACAACTTTGTCGTTTGAAACAATTACCTAACATCAAGCATACGTAGTATCTAATATATTTAATATTTGGTTATATCGAAAGAAGATAGTTCGATATATTATAGCATATTTCAAGTGTATCAGTTGTCAACATTAATCCAGTTAGAATGATTACCAACAGGTGGATGATATAAACGGAGAGAGAAAATGGAAGAAAGTTGAAGTGAATCTTGAAGAACATATCTTACTGCCAACATTCCCCTCAAATTTGTCAGTAGTTGATGACATCAATTATGATGCATATTTCAATAAATATATTACAAATTTGGGTGCCCAAGAGTTAGCCAAAAACAAACCCTTGTGGGAAATTCATATCATAAAGTATCCAACAAGTAACGCAGCTGCTAATTTAATTCTCAAGTTTCACCATTCACTTGGAGATGGCTATTCTGTGATGGGCCTTCTTATTTCAAGCATGAAAAGGGCAGACAATCCTTCTCTTCCCTTAACTTTTCCTTCAAGGAAAATTAATTCAGATGATCACAATAATAAAAATTTAAGGCATGTTTTAAATTATGTGCCTTCATTTATGAATAGTGTTTTAGATTTTGGATGGAGCATTATCAAAAGCAGTGTTCTTGAAGATGATTTGACACCTATAAGATCTGGTGTTGATGGTGTGGAGTTTAGGCCAGTTGCAATATGTACAATCTCATTCTCTCTTCATCAAATCAAACAAATCAAATCCAAACTTAGAGTGGTATGATTTAATTTTTCCCTTTATCTATGGATATAAAATTAACTATTGTTTAAAAACTTATTTAAGCAAATGTAGGACACACTCGAATAGAGTAGATTTGGCTAACGAAATGATGATGTGGTAATTTCATTTGGTATTTAGTTTTTAGTAGTTTTTTTTTTTATATATATATATATTGCAAATAAAATATTTATGTTTGCATTTTCCTACCTTGCATAATAGGGTTCTAATTTTTTATTTTTTTTTGAATTTTTAGAGCACACTCCAATTGTTTAGTTGAAATCTAAGGACAAAGTTAAGTTCTGAGAAATGATTTTATTAGTTTTGAGAATTTGTGATAAAAAAACATTTGACATTGAATAACTAAAACCTAGAACGAGTAACTTTCAAGCTAAGTTTTTCAATAAATTTGAAGAGTCGCAACACTTGAACCGGAAAATTACTCATTCTATACTTGAGCTAATCAATGTCAAATATTTTTTTTATCAATAGTTAAAAATAAGAAAATCAAACAATTACTAAATAAAACTTGAAACCCTAATTTGTTGGTTTTTTTCGAGATGAGTTTAGAATTTTTTTTCTTTTCCACAACATGGAAACCAATTAAAAAGTGGTTTTGAAATTGGTCAATTTCAAATTCAATCCAAGCTGCTTCAAGGCATAATGAGTTGAAATGTCTTGGAGTTAAATGCAACATCAAACGCCGTTTGACGCTACGCGGTGCTTGCTATACATGGCCCTGAAGCAATGTGCATGGTGACGCTCTTGCGTTCTCTATGTTTGGCTTTAGTGATTGCAAAAAAAAAAACTTTTCAAGATCAAAGACATGTCGATTTTAAAACTTCAAGGATTGAATAGCTGATACAGAATTGATCAAATCACTTTGCAACTTATAAATGTGAATTCATGTTTGTAGACAATCAACGATGTGATCACAGGAATAATATTTTTGGGAATTCGATTATACATGCATGAAACTTGTCCCGACTCAATCCAATCTAACTCAACAACAATTGTTTTACTCAACACAAGGATGTTTGGAAATTACAAGTCTGTAGAAGATATGCTAAAACCCAACTCAAATGCCCCATGGGGGAATCGCCTTGCCCTACTGCCTATTGACATTCCGAAACTCACTGATTATGAGCTTTCAAACCCAATCCAATTCATCAAAGCCGCACAAAAGTTGATCAAGAGGAAGAGATATTCTTATGCTACTTTCCTCCTTGACAAGCTCATGGAGATGGTGCATAAACTTAAAGGCCCAGAGGTATGTACGTAAATCTAATTTCTATTCTCATAATATATCTACTTTTGATCACATTTGTTGCATTTTAAAATCTAAAGATATTAACTAAATATTGTACTGCAACCCTTCAAGCTTGGTGTGGTCAAGTATAATTAGTTTATAAAACTTTTAGCATGTACGTATTAAAAATTTTGACGTAGGTAGCAGCCAAATACATGTACAAGATGGTTAGAAATTCAAGCTTAAGCATATCAAATATGATCGGACCAAAGGAAAAAATGGCTCTGCTTGGCCACCCGGCAAAAGGAGTCTACTTCATCCTTTTTGGTATACCTCAGGTAGGGTTGATATGAACGGTAATATTTTGCTATATTTAAAAATCTTTTCACTAGTTTTGTTATTTTAAAACAATTATCCTCTCACATGCTAAGGTTATAGTTAATAAATAATAGTTTTATTAAAAGAATTTAAACAAAAATACTTTTTTAAAAAATTATAAATTCGTCCATAGTTAATTCAAAACGTCCTAAGTATTTGTTATTATTTAAATGGAAGAAATATGGTACCATTCCAACTCTTAATTAAGTACATTGATGCAAAAGAACTTTAAATGATTTTTTACAAATATATAAACCGGAGAAGAATTGAATTTCTGACTTCTAAATTCATAATACAATACTTCACTAGCTAATTGTGCTATTCTTACATGTTCATGAGAGAATTATAATAATTTAGTTGTTTGTTAATATTTGCAGAGTTTGATAATAACAATGGTAAGCTACATGGAAAATTTGAGGATTGCCTTTGGGAGTGAGAAAGAATTTATAGATCAAGAGAAACTCACATCTTGTATGAAAAAAGCTTTCGAGCATATGTATAAGGCAGCTTCCGTTGATGTTTCCATTTAAAAATTGTATATCAATTGAAAGTTATGAGAATTGTTATGGATCAAAATATAGTAAAAATAAACT

Coding sequence (CDS)

ATGGGCCTTCTTATTTCAAGCATGAAAAGGGCAGACAATCCTTCTCTTCCCTTAACTTTTCCTTCAAGGAAAATTAATTCAGATGATCACAATAATAAAAATTTAAGGCATGTTTTAAATTATGTGCCTTCATTTATGAATAGTGTTTTAGATTTTGGATGGAGCATTATCAAAAGCAGTGTTCTTGAAGATGATTTGACACCTATAAGATCTGGTGTTGATGGTGTGGAGTTTAGGCCAGTTGCAATATGTACAATCTCATTCTCTCTTCATCAAATCAAACAAATCAAATCCAAACTTAGAGTGACAATCAACGATGTGATCACAGGAATAATATTTTTGGGAATTCGATTATACATGCATGAAACTTGTCCCGACTCAATCCAATCTAACTCAACAACAATTGTTTTACTCAACACAAGGATGTTTGGAAATTACAAGTCTGTAGAAGATATGCTAAAACCCAACTCAAATGCCCCATGGGGGAATCGCCTTGCCCTACTGCCTATTGACATTCCGAAACTCACTGATTATGAGCTTTCAAACCCAATCCAATTCATCAAAGCCGCACAAAAGTTGATCAAGAGGAAGAGATATTCTTATGCTACTTTCCTCCTTGACAAGCTCATGGAGATGGTGCATAAACTTAAAGGCCCAGAGCCAAATACATGTACAAGATGGTTAGAAATTCAAGCTTAA

Protein sequence

MGLLISSMKRADNPSLPLTFPSRKINSDDHNNKNLRHVLNYVPSFMNSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYMHETCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPNSNAPWGNRLALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPEPNTCTRWLEIQA

Homology

[Close]

BLAST of Cucsat.G9343.T12 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.2e-10
Identity = 59/245 (24.08%), Postives = 108/245 (44.08%), Query Frame = 0


Query: 1   MGLLISSMKRADN----PSLPLTFPSRK----INSDDHNNKNLRHVLNYVPSF----MNS 60
           M L+++ M++  N    PSLP  + S      + +   ++  L  ++  + +     +N+
Sbjct: 158 MSLVLACMRKTSNPDELPSLPYQYRSSSRSSLLTTGSRSDSRLLWLVKVIWTAVILGLNT 217

Query: 61  VLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVI 120
           V D    I+ +  ++D  TPI+      + + + +   + SL  IK  K+ + +TINDV+
Sbjct: 218 VCDALEFIVTTLFVKDTETPIKGDFLSTKSKQLRLVHRTVSLDDIKLTKNAMNMTINDVV 277

Query: 121 TGIIFLGIRLYM-------------HETCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKP 180
            G+   G+  Y+              +  P  I+  S   +L+N R     + + DM++ 
Sbjct: 278 LGVTQAGLSRYLARRYGEEETKNRKQKKLPKRIRLRSA--LLVNLRPTTGIQDLADMMEK 337

Query: 181 NSNAPWGN--RLALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMV 219
            S   WGN     + P  I    D     P++ ++ AQK I RK+ SY   L      ++
Sbjct: 338 GSKCRWGNWFGYVVFPFSIALRDD-----PLEHLEIAQKTISRKKNSYGAMLTYIFCRII 395

[Close]

BLAST of Cucsat.G9343.T12 vs. ExPASy Swiss-Prot
Match: Q94CK0 (Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=WSD7 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.1e-09
Identity = 52/191 (27.23%), Postives = 87/191 (45.55%), Query Frame = 0


Query: 47  NSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTIND 106
           N+ +D    +     L+D  TP+++G D V   P        SL  IK IK+ + +TIND
Sbjct: 199 NTTIDMLLLLATVLFLKDTKTPLKAGAD-VRSNPKRFYHRIISLDDIKLIKNAMNMTIND 258

Query: 107 VITGIIFLGIRLYMH-----------------ETCPDSIQSNSTTIVLLNTRMFGNYKSV 166
           V+ GI    +  Y++                    PD I+      V  N R    +K +
Sbjct: 259 VLFGITQASLSHYLNRQYGTKKEEDGALTSYRNNLPDGIRFRVACTV--NLRSDIGFKPL 318

Query: 167 EDMLKPNSNAPWGNRLA--LLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLD 219
            DM+  +S   WGN  +   LP  I   TD     P+ ++K ++ ++ RK++SY   L+ 
Sbjct: 319 ADMMVKDSKCRWGNYFSFIFLPFTIGLQTD-----PLVYLKMSKSMMARKKHSYHAALVY 378

[Close]

BLAST of Cucsat.G9343.T12 vs. ExPASy Swiss-Prot
Match: Q9FK89 (Wax ester synthase/diacylglycerol acyltransferase 9 OS=Arabidopsis thaliana OX=3702 GN=WSD9 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.6e-09
Identity = 57/245 (23.27%), Postives = 107/245 (43.67%), Query Frame = 0


Query: 1   MGLLISSMKRADNPSLPLTFPSRKINSDDHNNKNLRH------VLNYVPSFMNSVLDFGW 60
           + L+++S ++   P    T P  K     ++ +          V + V    N+++D   
Sbjct: 149 LSLILASTRKTSEPEAFSTLPVPKCRESYNHRRGFSFFRLVLVVCSTVRLIWNTLVDSFL 208

Query: 61  SIIKSSVLEDDLTPIRSGVDGVEFRPVAICTIS---FSLHQIKQIKSKLRVTINDVITGI 120
            +     L+D  TP++        +P AI   S    SL  IK IK+ + +TINDV+ G+
Sbjct: 209 CMATIFFLKDTDTPLKG-------KPGAIKKFSHRIVSLDDIKLIKNAMEMTINDVLLGV 268

Query: 121 IFLGIRLYMHETCPDSIQSNST----------------TIVLLNTRMFGNYKSVEDMLKP 180
               +  Y+H++   + +   T                +++++N R  G+ +S+ DM+  
Sbjct: 269 TEAALTRYLHQSYDKTNEEAGTSLTPNRQDLLDRIRLRSLIVVNLRPTGS-QSIADMMAK 328

Query: 181 NSNAPWGNRLA--LLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMV 219
            S   WGN ++  L P  I   +D     P+ ++   + +I RK+ S  T+++    E V
Sbjct: 329 GSKCRWGNYISVILFPFTIALQSD-----PLVYLSNVKSMIDRKKNSLITYIIYTFSEFV 380

[Close]

BLAST of Cucsat.G9343.T12 vs. ExPASy Swiss-Prot
Match: Q5KS41 (Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=WSD11 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 6.0e-09
Identity = 46/183 (25.14%), Postives = 83/183 (45.36%), Query Frame = 0


Query: 47  NSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTIND 106
           N+ +D     +   VL D  TP+ +   G E  P        S   +K +K+ +++T+ND
Sbjct: 207 NTFVDILMFALTIFVLRDTETPLLA-KPGSELIPKRFVHRIISFDDVKLVKNAMKMTVND 266

Query: 107 VITGIIFLGI-----RLYMHETCPDSIQS----NSTTIVLLNTRMFGNYKSVEDMLKPNS 166
           V+ G+   G+     R Y  E  P S +S       + +++N R     +++ DM+   S
Sbjct: 267 VLLGVTQAGLSRYLSRKYDQEATPKSKESMRRIRLRSAIMINLRPNAGIEALADMMAKKS 326

Query: 167 NAPWGNRLA--LLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHK 219
              WGN     LLP  +   TD     P+++++ A+  I RK++S          +++ K
Sbjct: 327 KCRWGNLFGYILLPFSVGLETD-----PLEYVRQAKATIDRKKHSLEAVFSMAFFKLILK 383

[Close]

BLAST of Cucsat.G9343.T12 vs. ExPASy Swiss-Prot
Match: Q9FFE8 (Wax ester synthase/diacylglycerol acyltransferase 8 OS=Arabidopsis thaliana OX=3702 GN=WSD8 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.3e-08
Identity = 52/198 (26.26%), Postives = 89/198 (44.95%), Query Frame = 0


Query: 47  NSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTIND 106
           N+++D          L+D  TP++ G + V   P      + SL  IK IK+ + +TIND
Sbjct: 199 NTLVDMFLLFATMLFLKDTKTPLKGGAN-VRSNPKTFYHRNISLDDIKLIKNAMNMTIND 258

Query: 107 VITGIIFLGI-----RLYMHE--------------TCPDSIQSNSTTIVLLNTRMFGNYK 166
           V+ GI    +     R Y HE                PD I+  +   V  N R    +K
Sbjct: 259 VLLGITQAALSSYLNRRYEHENNNEEDGVLTSYTNNLPDRIRFRAGCTV--NLRSDIGFK 318

Query: 167 SVEDMLKPNSNAPWGNRLA--LLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFL 224
            + +M+  +S   WGN  +  +LP+ I   TD     P+ ++  ++ ++ R ++SY   L
Sbjct: 319 PLAEMMVKDSKCRWGNYFSFIILPLSISLETD-----PLVYLNKSKAMMARTKHSYQAAL 378

[Close]

BLAST of Cucsat.G9343.T12 vs. NCBI nr
Match: XP_038888791.1 (O-acyltransferase WSD1-like [Benincasa hispida])

HSP 1 Score: 368 bits (945), Expect = 2.02e-123
Identity = 189/224 (84.38%), Postives = 203/224 (90.62%), Query Frame = 0


Query: 1   MGLLISSMKRADNPSLPLTFPSRKINSDDHNNKNLRHVLNYVPSF----MNSVLDFGWSI 60
           MGLLISSMKRADNPSLPLTFPSRKINSD  +N   R+VL+ VP F    MNSVLDFGWSI
Sbjct: 154 MGLLISSMKRADNPSLPLTFPSRKINSD--HNLQSRNVLSLVPKFVLSSMNSVLDFGWSI 213

Query: 61  IKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGI 120
           +KSSVLEDDLTPIRSG DGVEFRP+A  TI+FSLHQIKQIKSKLRVTINDV+TGIIFLGI
Sbjct: 214 MKSSVLEDDLTPIRSGGDGVEFRPIATWTINFSLHQIKQIKSKLRVTINDVVTGIIFLGI 273

Query: 121 RLYMHETCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPNSNAPWGNRLALLPIDIPKLT 180
           RLYM+ETCP+S QSNST IVLLNTRM G+YKS+EDMLKPNSN PWGN  ALLPIDIPKLT
Sbjct: 274 RLYMYETCPNSTQSNSTAIVLLNTRMLGDYKSIEDMLKPNSNNPWGNHFALLPIDIPKLT 333

Query: 181 DYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPE 220
           DYELSNPIQFIKAAQKLIKRKRYSYA FL+DKLMEM++KLKGPE
Sbjct: 334 DYELSNPIQFIKAAQKLIKRKRYSYAVFLVDKLMEMINKLKGPE 375

[Close]

BLAST of Cucsat.G9343.T12 vs. NCBI nr
Match: XP_038886812.1 (O-acyltransferase WSD1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 316 bits (809), Expect = 1.63e-103
Identity = 163/220 (74.09%), Postives = 185/220 (84.09%), Query Frame = 0


Query: 1   MGLLISSMKRADNPSLPLTFPSRKINSDDHNNKNLRHVLNYVPSFMNSVLDFGWSIIKSS 60
           MGLL+SSM RADNPSLPLTFPS KINS+  + +    V   + S +NSVLDFG S+IKSS
Sbjct: 153 MGLLLSSMTRADNPSLPLTFPSSKINSNQ-SRQIPSGVSQVLLSSINSVLDFGLSLIKSS 212

Query: 61  VLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYM 120
           VLED+LTPIRSG DGVEF+P +I T++FSLHQIKQIKSKLRVTINDVI G++FLGIRLYM
Sbjct: 213 VLEDELTPIRSGDDGVEFKPTSIWTMNFSLHQIKQIKSKLRVTINDVIAGVLFLGIRLYM 272

Query: 121 HETCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPNSNAPWGNRLALLPIDIPKLTDYEL 180
            ET PDS +SNST +VLLNTRMFG YK +EDML PNSN PWGNR A L IDIPKLTDYEL
Sbjct: 273 EETRPDSTKSNSTALVLLNTRMFGTYKCMEDMLNPNSNTPWGNRFAFLHIDIPKLTDYEL 332

Query: 181 SNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPE 220
           SNP+QF++AAQKLIKRKR S A FLLDKLMEM+HK +G E
Sbjct: 333 SNPLQFVEAAQKLIKRKRDSSAVFLLDKLMEMIHKFRGSE 371

[Close]

BLAST of Cucsat.G9343.T12 vs. NCBI nr
Match: XP_038886811.1 (O-acyltransferase WSD1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 316 bits (809), Expect = 6.61e-103
Identity = 163/220 (74.09%), Postives = 185/220 (84.09%), Query Frame = 0


Query: 1   MGLLISSMKRADNPSLPLTFPSRKINSDDHNNKNLRHVLNYVPSFMNSVLDFGWSIIKSS 60
           MGLL+SSM RADNPSLPLTFPS KINS+  + +    V   + S +NSVLDFG S+IKSS
Sbjct: 153 MGLLLSSMTRADNPSLPLTFPSSKINSNQ-SRQIPSGVSQVLLSSINSVLDFGLSLIKSS 212

Query: 61  VLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYM 120
           VLED+LTPIRSG DGVEF+P +I T++FSLHQIKQIKSKLRVTINDVI G++FLGIRLYM
Sbjct: 213 VLEDELTPIRSGDDGVEFKPTSIWTMNFSLHQIKQIKSKLRVTINDVIAGVLFLGIRLYM 272

Query: 121 HETCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPNSNAPWGNRLALLPIDIPKLTDYEL 180
            ET PDS +SNST +VLLNTRMFG YK +EDML PNSN PWGNR A L IDIPKLTDYEL
Sbjct: 273 EETRPDSTKSNSTALVLLNTRMFGTYKCMEDMLNPNSNTPWGNRFAFLHIDIPKLTDYEL 332

Query: 181 SNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPE 220
           SNP+QF++AAQKLIKRKR S A FLLDKLMEM+HK +G E
Sbjct: 333 SNPLQFVEAAQKLIKRKRDSSAVFLLDKLMEMIHKFRGSE 371

[Close]

BLAST of Cucsat.G9343.T12 vs. NCBI nr
Match: XP_022969083.1 (O-acyltransferase WSD1-like [Cucurbita maxima])

HSP 1 Score: 308 bits (790), Expect = 4.60e-100
Identity = 160/221 (72.40%), Postives = 184/221 (83.26%), Query Frame = 0


Query: 1   MGLLISSMKRADNPSLPLTFPSRKINSDDHNNKNLRHVLNYVPSFMNSVLDFGWSIIKSS 60
           MG+LISSM RA +PSLPLTFPSRK+  +   +  L  V  ++ S MN+VLDFG +I+KSS
Sbjct: 150 MGVLISSMTRAHDPSLPLTFPSRKVKLEQSGHI-LGRVYQFLFSSMNTVLDFGGNIMKSS 209

Query: 61  VLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYM 120
           VLED LTPIRSG DGVEFR  AI T +FSLHQIKQIK+KL VT+NDVITG+IFLG+RLYM
Sbjct: 210 VLEDHLTPIRSGGDGVEFRTFAIRTFTFSLHQIKQIKAKLGVTVNDVITGMIFLGLRLYM 269

Query: 121 HETCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPN-SNAPWGNRLALLPIDIPKLTDYE 180
            ETCPDS  SNST +VL+NTRM  NYKSV+DMLKPN SNAPWGNR A L IDIPK T+YE
Sbjct: 270 QETCPDSTNSNSTALVLINTRMVENYKSVQDMLKPNNSNAPWGNRFAFLHIDIPKATEYE 329

Query: 181 LSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPE 220
           LSNP+QFIKAAQK+IKRKRYS A FL+ KLMEM+HKL+GPE
Sbjct: 330 LSNPLQFIKAAQKMIKRKRYSSAVFLVGKLMEMIHKLRGPE 369

[Close]

BLAST of Cucsat.G9343.T12 vs. NCBI nr
Match: XP_004136846.2 (O-acyltransferase WSD1 [Cucumis sativus] >KGN43650.1 hypothetical protein Csa_017285 [Cucumis sativus])

HSP 1 Score: 303 bits (776), Expect = 5.95e-98
Identity = 156/224 (69.64%), Postives = 181/224 (80.80%), Query Frame = 0


Query: 1   MGLLISSMKRADNPSLPLTFPSRKINSDDHNNKNLRHVLNYVPSFM----NSVLDFGWSI 60
           MGLL+S+MKRADNPSLPLTFP    N    N+K L  + + V  F+    NSVLDFG S+
Sbjct: 149 MGLLLSTMKRADNPSLPLTFP---FNGKMINSKKLGQISSGVSHFLLSSINSVLDFGLSL 208

Query: 61  IKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGI 120
           IKSSVLED+LTPIRSG DGVEF+P  I TI+FSLHQIKQIKSKLRVT+NDV+ G++FLGI
Sbjct: 209 IKSSVLEDELTPIRSGGDGVEFKPTEIWTITFSLHQIKQIKSKLRVTVNDVVAGMLFLGI 268

Query: 121 RLYMHETCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPNSNAPWGNRLALLPIDIPKLT 180
           RLYM ET PDS +SNST +VLLNTRMFG YK +EDML PNSN PWGNR   L IDIPKLT
Sbjct: 269 RLYMEETHPDSTKSNSTALVLLNTRMFGTYKCMEDMLNPNSNTPWGNRFGFLHIDIPKLT 328

Query: 181 DYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPE 220
           D+ LSNP+QF++AAQKLIKRKR S A FL+DKLME++HK +G E
Sbjct: 329 DFNLSNPLQFVQAAQKLIKRKRDSSAVFLVDKLMEIIHKFRGSE 369

[Close]

BLAST of Cucsat.G9343.T12 vs. ExPASy TrEMBL
Match: A0A6J1HZY6 (Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468185 PE=4 SV=1)

HSP 1 Score: 308 bits (790), Expect = 2.22e-100
Identity = 160/221 (72.40%), Postives = 184/221 (83.26%), Query Frame = 0


Query: 1   MGLLISSMKRADNPSLPLTFPSRKINSDDHNNKNLRHVLNYVPSFMNSVLDFGWSIIKSS 60
           MG+LISSM RA +PSLPLTFPSRK+  +   +  L  V  ++ S MN+VLDFG +I+KSS
Sbjct: 150 MGVLISSMTRAHDPSLPLTFPSRKVKLEQSGHI-LGRVYQFLFSSMNTVLDFGGNIMKSS 209

Query: 61  VLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYM 120
           VLED LTPIRSG DGVEFR  AI T +FSLHQIKQIK+KL VT+NDVITG+IFLG+RLYM
Sbjct: 210 VLEDHLTPIRSGGDGVEFRTFAIRTFTFSLHQIKQIKAKLGVTVNDVITGMIFLGLRLYM 269

Query: 121 HETCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPN-SNAPWGNRLALLPIDIPKLTDYE 180
            ETCPDS  SNST +VL+NTRM  NYKSV+DMLKPN SNAPWGNR A L IDIPK T+YE
Sbjct: 270 QETCPDSTNSNSTALVLINTRMVENYKSVQDMLKPNNSNAPWGNRFAFLHIDIPKATEYE 329

Query: 181 LSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPE 220
           LSNP+QFIKAAQK+IKRKRYS A FL+ KLMEM+HKL+GPE
Sbjct: 330 LSNPLQFIKAAQKMIKRKRYSSAVFLVGKLMEMIHKLRGPE 369

[Close]

BLAST of Cucsat.G9343.T12 vs. ExPASy TrEMBL
Match: A0A0A0K1W0 (Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051300 PE=4 SV=1)

HSP 1 Score: 303 bits (776), Expect = 2.88e-98
Identity = 156/224 (69.64%), Postives = 181/224 (80.80%), Query Frame = 0


Query: 1   MGLLISSMKRADNPSLPLTFPSRKINSDDHNNKNLRHVLNYVPSFM----NSVLDFGWSI 60
           MGLL+S+MKRADNPSLPLTFP    N    N+K L  + + V  F+    NSVLDFG S+
Sbjct: 149 MGLLLSTMKRADNPSLPLTFP---FNGKMINSKKLGQISSGVSHFLLSSINSVLDFGLSL 208

Query: 61  IKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGI 120
           IKSSVLED+LTPIRSG DGVEF+P  I TI+FSLHQIKQIKSKLRVT+NDV+ G++FLGI
Sbjct: 209 IKSSVLEDELTPIRSGGDGVEFKPTEIWTITFSLHQIKQIKSKLRVTVNDVVAGMLFLGI 268

Query: 121 RLYMHETCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPNSNAPWGNRLALLPIDIPKLT 180
           RLYM ET PDS +SNST +VLLNTRMFG YK +EDML PNSN PWGNR   L IDIPKLT
Sbjct: 269 RLYMEETHPDSTKSNSTALVLLNTRMFGTYKCMEDMLNPNSNTPWGNRFGFLHIDIPKLT 328

Query: 181 DYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPE 220
           D+ LSNP+QF++AAQKLIKRKR S A FL+DKLME++HK +G E
Sbjct: 329 DFNLSNPLQFVQAAQKLIKRKRDSSAVFLVDKLMEIIHKFRGSE 369

[Close]

BLAST of Cucsat.G9343.T12 vs. ExPASy TrEMBL
Match: A0A6J1GLS6 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455511 PE=4 SV=1)

HSP 1 Score: 301 bits (771), Expect = 1.64e-97
Identity = 159/221 (71.95%), Postives = 183/221 (82.81%), Query Frame = 0


Query: 1   MGLLISSMKRADNPSLPLTFPSRKINSDDHNNKNLRHVLNYVPSFMNSVLDFGWSIIKSS 60
           MG+LISSM RA +PSLPLTFPSRK+  +   +  L  V  ++ S MN+VLDFG +++KSS
Sbjct: 150 MGVLISSMTRAHDPSLPLTFPSRKVKLEQSGHI-LGRVYQFLLSSMNTVLDFGGNMMKSS 209

Query: 61  VLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYM 120
           VLED LTPIRSG DGVEFR  AI TI+FSLH IK+IK+KL VTINDVITG+IFLG+RLYM
Sbjct: 210 VLEDHLTPIRSGGDGVEFRTFAIRTITFSLHHIKKIKAKLGVTINDVITGMIFLGLRLYM 269

Query: 121 HETCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPN-SNAPWGNRLALLPIDIPKLTDYE 180
            ET PDS  SNST +VL+NTRM  NYKSV+DMLKPN SNAPWGNR ALL IDIPK TD E
Sbjct: 270 QETRPDSTNSNSTALVLINTRMVENYKSVQDMLKPNNSNAPWGNRFALLHIDIPKATDCE 329

Query: 181 LSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPE 220
           LSNP+QFIKAAQK+IKRKRYS A FL+ KLMEM+HKL+GPE
Sbjct: 330 LSNPLQFIKAAQKMIKRKRYSSAVFLVGKLMEMIHKLRGPE 369

[Close]

BLAST of Cucsat.G9343.T12 vs. ExPASy TrEMBL
Match: A0A6J1GJA7 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454845 PE=4 SV=1)

HSP 1 Score: 295 bits (754), Expect = 5.99e-95
Identity = 154/221 (69.68%), Postives = 179/221 (81.00%), Query Frame = 0


Query: 1   MGLLISSMKRADNPSLPLTFPSRKINSDDHNNKNLRHVLNYVPSFMNSVLDFGWSIIKSS 60
           MGLLIS M RA NPSLPLTFPSR I ++   +  L  V  ++ S MN+VLDFGW+I+KSS
Sbjct: 150 MGLLISGMTRAHNPSLPLTFPSRTIKAEQSGHI-LGRVSQFLLSSMNTVLDFGWNIMKSS 209

Query: 61  VLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYM 120
           VLED LTPIRSG D VEFR  AI TI+FSL QIKQIK+KL  TINDVITG+IFLG+RLYM
Sbjct: 210 VLEDHLTPIRSGRDDVEFRTFAIRTITFSLPQIKQIKTKLGETINDVITGMIFLGLRLYM 269

Query: 121 HETCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPN-SNAPWGNRLALLPIDIPKLTDYE 180
            ET PDS  SNST +VL+NTRM GNYKS++DMLK N SN  WGNR   L IDIPK T+YE
Sbjct: 270 QETRPDSTNSNSTALVLINTRMIGNYKSLQDMLKANNSNTSWGNRFGFLHIDIPKATEYE 329

Query: 181 LSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPE 220
           LSNP+QFIKAAQ++IKRKRYS A +L+DK+MEM+HKL+GPE
Sbjct: 330 LSNPLQFIKAAQEMIKRKRYSSAVYLVDKVMEMIHKLRGPE 369

[Close]

BLAST of Cucsat.G9343.T12 vs. ExPASy TrEMBL
Match: A0A1S4E0D6 (Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495689 PE=4 SV=1)

HSP 1 Score: 299 bits (766), Expect = 2.82e-94
Identity = 151/173 (87.28%), Postives = 157/173 (90.75%), Query Frame = 0


Query: 48  SVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDV 107
           S   F     KSSVLEDDLTPIRSG DGVEFRPV ICTISFSLHQIKQIKSKLRVTINDV
Sbjct: 463 SCFRFWMEHFKSSVLEDDLTPIRSGGDGVEFRPVEICTISFSLHQIKQIKSKLRVTINDV 522

Query: 108 ITGIIFLGIRLYMHETCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPNSNAPWGNRLAL 167
           I+GI+F+GIRLYMHETCPDS QSN TTIVLLNTRMFG+YKS+EDMLKPNSNA WGNR+AL
Sbjct: 523 ISGIVFMGIRLYMHETCPDSTQSNVTTIVLLNTRMFGDYKSIEDMLKPNSNASWGNRIAL 582

Query: 168 LPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPE 220
           L IDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYA FLLDKLMEMV KLKGPE
Sbjct: 583 LLIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYAIFLLDKLMEMVQKLKGPE 635

The following BLAST results are available for this feature:

BLAST of Cucsat.G9343.T12 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M3B3	3.2e-10	24.08	Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3...	[more]
Q94CK0	2.1e-09	27.23	Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3...	[more]
Q9FK89	4.6e-09	23.27	Wax ester synthase/diacylglycerol acyltransferase 9 OS=Arabidopsis thaliana OX=3...	[more]
Q5KS41	6.0e-09	25.14	Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=...	[more]
Q9FFE8	2.3e-08	26.26	Wax ester synthase/diacylglycerol acyltransferase 8 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Cucsat.G9343.T12 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888791.1	2.02e-123	84.38	O-acyltransferase WSD1-like [Benincasa hispida]	[more]
XP_038886812.1	1.63e-103	74.09	O-acyltransferase WSD1-like isoform X2 [Benincasa hispida]	[more]
XP_038886811.1	6.61e-103	74.09	O-acyltransferase WSD1-like isoform X1 [Benincasa hispida]	[more]
XP_022969083.1	4.60e-100	72.40	O-acyltransferase WSD1-like [Cucurbita maxima]	[more]
XP_004136846.2	5.95e-98	69.64	O-acyltransferase WSD1 [Cucumis sativus] >KGN43650.1 hypothetical protein Csa_01...	[more]

BLAST of Cucsat.G9343.T12 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HZY6	2.22e-100	72.40	Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468185 PE=...	[more]
A0A0A0K1W0	2.88e-98	69.64	Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051300 PE=4...	[more]
A0A6J1GLS6	1.64e-97	71.95	Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455511 P...	[more]
A0A6J1GJA7	5.99e-95	69.68	Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454845 P...	[more]
A0A1S4E0D6	2.82e-94	87.28	Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495689 PE=4 SV...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR045034	O-acyltransferase WSD1-like	PANTHER	PTHR31650	O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN	coord: 1..220
None	No IPR available	PANTHER	PTHR31650:SF34	O-ACYLTRANSFERASE WSD1-LIKE	coord: 1..220

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G9343	Cucsat.G9343	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9343.T12.E1	Cucsat.G9343.T12.E1	exon
Cucsat.G9343.T12.E2	Cucsat.G9343.T12.E2	exon
Cucsat.G9343.T12.E3	Cucsat.G9343.T12.E3	exon
Cucsat.G9343.T12.E4	Cucsat.G9343.T12.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9343.T12.C3	Cucsat.G9343.T12.C3	CDS
Cucsat.G9343.T12.C2	Cucsat.G9343.T12.C2	CDS
Cucsat.G9343.T12.C1	Cucsat.G9343.T12.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G9343.T12	Cucsat.G9343.T12-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045017	glycerolipid biosynthetic process
biological_process	GO:0019432	triglyceride biosynthetic process
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0008374	O-acyltransferase activity
molecular_function	GO:0004144	diacylglycerol O-acyltransferase activity
molecular_function	GO:0047196	long-chain-alcohol O-fatty-acyltransferase activity