Cucsat.G9343 (gene) Cucumber (B10) v3

BLAST of Cucsat.G9343 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.2e-49
Identity = 133/460 (28.91%), Postives = 236/460 (51.30%), Query Frame = 0

Query: 7   EEEEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMV 66
           E+EEE  +P+SP A+ F++   + ++I+V+ F+  +D    +   K+    +P FSS +V
Sbjct: 13  EKEEE--QPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFIRHPRFSSKLV 72

Query: 67  DDINGE-RKWKKVEVNLEEHILLPTF-PSNLSVVDDINYDAYFNKYITNLGAQELAKNKP 126
            D NG+ ++W +  V +E+H ++P   P N+      N +A+   Y+++L    L  ++P
Sbjct: 73  TDENGQNQRWVRTNVVVEDHFIVPKIKPQNIE-----NSNAFLEDYVSDLMKIPLDTSRP 132

Query: 127 LWEIHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADN----PSLPLTFPSRK 186
           LWE+H++   TS+A    +LK HHS+GDG S+M L+++ M++  N    PSLP  + S  
Sbjct: 133 LWELHLLDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSS 192

Query: 187 ----INSDDHNNKNLRHVLNYVPSF----MNSVLDFGWSIIKSSVLEDDLTPIRSGVDGV 246
               + +   ++  L  ++  + +     +N+V D    I+ +  ++D  TPI+      
Sbjct: 193 RSSLLTTGSRSDSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVKDTETPIKGDFLST 252

Query: 247 EFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYM-------------HET 306
           + + + +   + SL  IK  K+ + +TINDV+ G+   G+  Y+              + 
Sbjct: 253 KSKQLRLVHRTVSLDDIKLTKNAMNMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKK 312

Query: 307 CPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPNSNAPWGN--RLALLPIDIPKLTDYELS 366
            P  I+  S   +L+N R     + + DM++  S   WGN     + P  I    D    
Sbjct: 313 LPKRIRLRSA--LLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFPFSIALRDD---- 372

Query: 367 NPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPEVAAKYMYKMVRNSSLSISNMI 426
            P++ ++ AQK I RK+ SY   L      ++ KL G +VAA  + +MV N++++ SNM+
Sbjct: 373 -PLEHLEIAQKTISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMV 432

Query: 427 GPKEKMALLGHPAKGVYFILFGIPQSLIITMVSYMENLRI 438
           GP E+++  GHP        +G P +L I   SYM  + I
Sbjct: 433 GPVEEVSFYGHPITYFASSAYGHPHALTIHCQSYMNKMTI 458

BLAST of Cucsat.G9343 vs. ExPASy Swiss-Prot
Match: Q9FK89 (Wax ester synthase/diacylglycerol acyltransferase 9 OS=Arabidopsis thaliana OX=3702 GN=WSD9 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.9e-46
Identity = 134/490 (27.35%), Postives = 243/490 (49.59%), Query Frame = 0

Query: 1   MDQFKKEEEEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPL 60
           M++  KEEEE   EP+SP A+ F   ++   ++     +  I+   I+   K  +  +P 
Sbjct: 1   MEKKMKEEEE---EPLSPMARAFQEPSIDCGIVIKFGCKTKINPDVIVDSLKLNVFKHPR 60

Query: 61  FSSIMVDDINGERKWKKVE-VNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQEL 120
           F S++ DD     KW + + VN+EEH+ +P     L+  D    + +   YI+++    L
Sbjct: 61  FCSLLDDD---GTKWLRTDVVNVEEHVFVPDIDPKLTEED---VEWFVEDYISSITMIPL 120

Query: 121 AKNKPLWEIHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSR 180
            + KPLWE+HI+   TS+A A  +++ HH+LGDG S++ L+++S ++   P    T P  
Sbjct: 121 DRTKPLWEVHILNAKTSDAEAICVIRCHHALGDGVSILSLILASTRKTSEPEAFSTLPVP 180

Query: 181 KINSDDHNNKNLRH------VLNYVPSFMNSVLDFGWSIIKSSVLEDDLTPIRSGVDGVE 240
           K     ++ +          V + V    N+++D    +     L+D  TP++       
Sbjct: 181 KCRESYNHRRGFSFFRLVLVVCSTVRLIWNTLVDSFLCMATIFFLKDTDTPLKG------ 240

Query: 241 FRPVAICTIS---FSLHQIKQIKSKLRVTINDVITGIIFLGIRLYMHETCPDSIQSNST- 300
            +P AI   S    SL  IK IK+ + +TINDV+ G+    +  Y+H++   + +   T 
Sbjct: 241 -KPGAIKKFSHRIVSLDDIKLIKNAMEMTINDVLLGVTEAALTRYLHQSYDKTNEEAGTS 300

Query: 301 ---------------TIVLLNTRMFGNYKSVEDMLKPNSNAPWGNRLA--LLPIDIPKLT 360
                          +++++N R  G+ +S+ DM+   S   WGN ++  L P  I   +
Sbjct: 301 LTPNRQDLLDRIRLRSLIVVNLRPTGS-QSIADMMAKGSKCRWGNYISVILFPFTIALQS 360

Query: 361 DYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPEVAAKYMYKMVRNSSLS 420
           D     P+ ++   + +I RK+ S  T+++    E V K  G  VA  +  K++ N+++ 
Sbjct: 361 D-----PLVYLSNVKSMIDRKKNSLITYIIYTFSEFVIKAFGINVAVAFQRKIMLNTTMC 420

Query: 421 ISNMIGPKEKMALLGHPAKGVYFILFGIPQSLIITMVSYMENLRIAFGSEKEFIDQEKLT 463
           ISN+ GP E+++  GHP       ++G+PQ+L I  +SY   + I+   +   ID  KL 
Sbjct: 421 ISNLPGPTEEVSFHGHPIAYFAPSIYGLPQALTIHYLSYANKMIISVAVDPMIIDAHKLC 468

BLAST of Cucsat.G9343 vs. ExPASy Swiss-Prot
Match: Q94CK0 (Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=WSD7 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.2e-45
Identity = 136/486 (27.98%), Postives = 243/486 (50.00%), Query Frame = 0

Query: 14  EPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMVDDINGER 73
           EPVSP A+ F S  + +  + ++ F+  I+   ++   K  +  +P FSSI+ D  NG  
Sbjct: 7   EPVSPMARVFQSPGIDLCAVTIMGFKTKINPDVVLDALKQNVSKHPRFSSILSD--NG-A 66

Query: 74  KWKKVEVNLEEHILLPTFPSNLSVVDDINY--DAYFNKYITNLGAQELAKNKPLWEIHII 133
           KW + EVN+E+H+++P   +     ++I     ++ + Y++ L    L +++PLW+IHI+
Sbjct: 67  KWIETEVNVEDHVIVPYIDA-----EEIGEGGQSFIDDYMSRLTMIPLDRSRPLWDIHIL 126

Query: 134 KYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSL-PLTFPSRKINSDDHNNKN 193
              TS A A   ++ HHSLGDG S++ L+++   +  +P +     PS K          
Sbjct: 127 NVKTSEAEAVGFIRSHHSLGDGMSLISLMLACTHKTSDPDMFSNAIPSMK------RRAT 186

Query: 194 LRHVLNYVPSFMNSVLDFG------WS------IIKSSV--LEDDLTPIRSGVDGVEFRP 253
           + H L     F+ S+   G      W+      ++ ++V  L+D  TP+++G D V   P
Sbjct: 187 MSHSLKTKGWFLRSIFTIGSTMRLLWNTTIDMLLLLATVLFLKDTKTPLKAGAD-VRSNP 246

Query: 254 VAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYMH-----------------ET 313
                   SL  IK IK+ + +TINDV+ GI    +  Y++                   
Sbjct: 247 KRFYHRIISLDDIKLIKNAMNMTINDVLFGITQASLSHYLNRQYGTKKEEDGALTSYRNN 306

Query: 314 CPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPNSNAPWGNRLA--LLPIDIPKLTDYELS 373
            PD I+      V  N R    +K + DM+  +S   WGN  +   LP  I   TD    
Sbjct: 307 LPDGIRFRVACTV--NLRSDIGFKPLADMMVKDSKCRWGNYFSFIFLPFTIGLQTD---- 366

Query: 374 NPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPEVAAKYMYKMVRNSSLSISNMI 433
            P+ ++K ++ ++ RK++SY   L+  ++++V K+ G + AA+   + VRN++  +SN+I
Sbjct: 367 -PLVYLKMSKSMMARKKHSYHAALVYFIIKIVLKVFGAKAAAELFDRPVRNTTTCVSNVI 426

Query: 434 GPKEKMALLGHPAKGVYFILFGIPQSLIITMVSYMENLRIAFGSEKEFI-DQEKLTSCMK 463
           GP E+++  GHP   +    +G   +L+I ++SY + + I+   +   I D  K+   M+
Sbjct: 427 GPMEEISFRGHPVSYIAPSSYGHSHALLIHLMSYADKMIISLAYDPTVISDPHKICDDME 470

BLAST of Cucsat.G9343 vs. ExPASy Swiss-Prot
Match: Q5KS41 (Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=WSD11 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.8e-45
Identity = 136/493 (27.59%), Postives = 245/493 (49.70%), Query Frame = 0

Query: 1   MDQFKKEEEEEL-LEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNP 60
           M + KK   E +  EP+SP ++ FNS   + ++I  +  ++  D   I+   +  +  +P
Sbjct: 1   MGEDKKTARETVEEEPLSPCSRLFNSPDFNCAIIVTMGSKVKGDTPAIIHGLEHTLVNHP 60

Query: 61  LFSSIMVDDINGERKWKKVEVNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQEL 120
            FSSI+  +   + +W + +V +EEH+++P    ++      N D Y   YI+ L    +
Sbjct: 61  RFSSILEMNNGKKPRWVRTKVKVEEHVIVPDVDPDIE-----NPDQYLEDYISKLTTIPM 120

Query: 121 AKNKPLWEIHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNP-SLPLTFPS 180
             +KPLWE+H++   TSNA +  ILK HHSLGDG S+M LL++  ++  +P +LP     
Sbjct: 121 DLSKPLWEMHLLGVKTSNAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVAVH 180

Query: 181 RK-----INSDDHNN-----KNLRHVLNYVPSFMNSVLDFGWSIIKSSVLEDDLTPIRSG 240
           +K      NS   N        L  +L  + +    +L F  +I    VL D  TP+ + 
Sbjct: 181 KKRFGPSCNSGFFNKIWWLFVGLWFILRLLFNTFVDILMFALTIF---VLRDTETPLLA- 240

Query: 241 VDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGI-----RLYMHETCPDS 300
             G E  P        S   +K +K+ +++T+NDV+ G+   G+     R Y  E  P S
Sbjct: 241 KPGSELIPKRFVHRIISFDDVKLVKNAMKMTVNDVLLGVTQAGLSRYLSRKYDQEATPKS 300

Query: 301 IQS----NSTTIVLLNTRMFGNYKSVEDMLKPNSNAPWGNRLA--LLPIDIPKLTDYELS 360
            +S       + +++N R     +++ DM+   S   WGN     LLP  +   TD    
Sbjct: 301 KESMRRIRLRSAIMINLRPNAGIEALADMMAKKSKCRWGNLFGYILLPFSVGLETD---- 360

Query: 361 NPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPEVAAKYMYKMVRNSSLSISNMI 420
            P+++++ A+  I RK++S          +++ K+ G + +   + K++ +++LS SN++
Sbjct: 361 -PLEYVRQAKATIDRKKHSLEAVFSMAFFKLILKVLGLKASVVLVRKVIHSTTLSFSNVV 420

Query: 421 GPKEKMALLGHPAKGVYFILFGIPQSLIITMVSYMENLRIAFGSEKEFI-DQEKLTSCMK 470
           GPKE++   GHP   +   +FG P +L +   +Y   + I+  ++   I D  K+   + 
Sbjct: 421 GPKEEITFHGHPLNYISPCVFGHPHALTLHFQTYANKVIISVTADPTVIPDPHKMCDDLV 478

BLAST of Cucsat.G9343 vs. ExPASy Swiss-Prot
Match: Q9M3B1 (Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=WSD6 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.4e-44
Identity = 136/503 (27.04%), Postives = 246/503 (48.91%), Query Frame = 0

Query: 5   KKEEEEELLE--PVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFS 64
           +K++EEE+ E  P+SP A+ F++   +  VI+V+  +  ID   I+   K  +  +P FS
Sbjct: 15  RKDDEEEVEEEQPLSPAARVFHAPEFNCYVISVIGIKKKIDPDVIIEGLKQTLIRHPRFS 74

Query: 65  SIMVDDINGERK-----WKKVEVNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQ 124
           S MV    G +K     W +  V + +H+++    +    +++ N DA+   Y++NL   
Sbjct: 75  SKMVSTSVGNKKRQTQSWVRTNVVVTDHVIVSDIQT--QNIENGNADAFLETYVSNLTTV 134

Query: 125 ELAKNKPLWEIHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFP 184
            L  +KPLW++H++   TS+A    +LKFHHSLGDG S+M L+++ M++  NP    + P
Sbjct: 135 PLDISKPLWQLHLLDLKTSDAENVAVLKFHHSLGDGMSLMALVLACMRKTSNPDELPSLP 194

Query: 185 SRKINS------------DDHNNKNLRHVLNYVPSFMNSVLDFGWSIIKSSVLEDDLTPI 244
           ++  +S            D      +  + + +   +N+V D    I  +  L+D  TPI
Sbjct: 195 NQNRSSSRSSRLMAGSRGDSRFLWLVMVIWSAIMLVLNTVCDALEFIATTMFLKDTETPI 254

Query: 245 RSGVDGVEFRPVAICTISFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYMHE------- 304
           +      + + + +   + SL  IK IK+ +++T+NDV+ G+   G+  Y+         
Sbjct: 255 KGDFRFSKSKRMCLVHRTVSLDDIKLIKNTMKMTVNDVVLGVSQAGLSQYLDRRYGEKKK 314

Query: 305 -------------TCPDSIQSNSTTIVLLNTRMFGNYKSVEDMLKPNSNAPWGNRLA--L 364
                          P  I+  S   +L+N R     + + DM+   S   WGN +   +
Sbjct: 315 KVGEDQDSKRKATDMPKRIRLRSA--LLVNLRPNTGIQDLADMMAKGSTCRWGNWIGYIV 374

Query: 365 LPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLMEMVHKLKGPEVAAKYMY 424
            P  I    D     P+Q ++ A+++I RK+ S    L     + + K  G +VAAK + 
Sbjct: 375 FPFSIGLRDD-----PLQHLRRAKRIIDRKKNSLEAALTFVAGKFILKTFGVQVAAKIIN 434

Query: 425 KMVRNSSLSISNMIGPKEKMALLGHPAKGVYFILFGIPQSLIITMVSYMENLRIAFGSEK 466
           + + N+++S SN+IGP E+++  GHP   +   ++G P +L +   SYM  + I+   + 
Sbjct: 435 RALSNTTMSFSNLIGPIEEISFYGHPITYMAPSVYGHPHALTMHFQSYMNQMTISLTVDP 494

BLAST of Cucsat.G9343 vs. NCBI nr
Match: XP_038888791.1 (O-acyltransferase WSD1-like [Benincasa hispida])

HSP 1 Score: 755 bits (1950), Expect = 4.70e-272
Identity = 385/470 (81.91%), Postives = 424/470 (90.21%), Query Frame = 0

Query: 7   EEEEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMV 66
           E+E+ELLEPVSP AQCFN+S LSISVI VLEFEIPIDE +IM YAKDLIPLNPLFSSIMV
Sbjct: 5   EQEDELLEPVSPVAQCFNTSVLSISVIVVLEFEIPIDEAKIMSYAKDLIPLNPLFSSIMV 64

Query: 67  DDINGERKWKKVEVNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPLW 126
           +DING+RKWKKVEVNLEEHIL+PT PSNLS+V+ +NYD YF +Y+T+LG QEL KNKPLW
Sbjct: 65  EDINGKRKWKKVEVNLEEHILVPTLPSNLSLVE-LNYDTYFKEYMTDLGVQELPKNKPLW 124

Query: 127 EIHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSRKINSDDH 186
           EIHII YPT NAAA+LILKFHHSLGDGYS+MGLLISSMKRADNPSLPLTFPSRKINSD  
Sbjct: 125 EIHIINYPTKNAAAHLILKFHHSLGDGYSMMGLLISSMKRADNPSLPLTFPSRKINSD-- 184

Query: 187 NNKNLRHVLNYVPSF----MNSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTI 246
           +N   R+VL+ VP F    MNSVLDFGWSI+KSSVLEDDLTPIRSG DGVEFRP+A  TI
Sbjct: 185 HNLQSRNVLSLVPKFVLSSMNSVLDFGWSIMKSSVLEDDLTPIRSGGDGVEFRPIATWTI 244

Query: 247 SFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYMHETCPDSIQSNSTTIVLLNTRMFGNY 306
           +FSLHQIKQIKSKLRVTINDV+TGIIFLGIRLYM+ETCP+S QSNST IVLLNTRM G+Y
Sbjct: 245 NFSLHQIKQIKSKLRVTINDVVTGIIFLGIRLYMYETCPNSTQSNSTAIVLLNTRMLGDY 304

Query: 307 KSVEDMLKPNSNAPWGNRLALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLL 366
           KS+EDMLKPNSN PWGN  ALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYA FL+
Sbjct: 305 KSIEDMLKPNSNNPWGNHFALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYAVFLV 364

Query: 367 DKLMEMVHKLKGPEVAAKYMYKMVRNSSLSISNMIGPKEKMALLGHPAKGVYFILFGIPQ 426
           DKLMEM++KLKGPE AAKY+YK  RNSS+SISNMIGPKEKMALLGHPAKG+YF + G+PQ
Sbjct: 365 DKLMEMINKLKGPEAAAKYVYKTARNSSISISNMIGPKEKMALLGHPAKGIYFTVVGLPQ 424

Query: 427 SLIITMVSYMENLRIAFGSEKEFIDQEKLTSCMKKAFEHMYKAASVDVSI 472
           SL+ITM+SYMENLRI FGSEKEFIDQ KLTSCMKKA E ++ A S D+SI
Sbjct: 425 SLMITMISYMENLRITFGSEKEFIDQVKLTSCMKKALELIHNA-SFDISI 470

BLAST of Cucsat.G9343 vs. NCBI nr
Match: XP_038886811.1 (O-acyltransferase WSD1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 680 bits (1754), Expect = 2.74e-242
Identity = 352/470 (74.89%), Postives = 405/470 (86.17%), Query Frame = 0

Query: 3   QFKKEEEEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFS 62
           + KKE+E+E LEPVSPTAQ FN+SALSISVIAVLEFEIPIDE +IM YAKDLIPLNPLFS
Sbjct: 2   EIKKEQEDEWLEPVSPTAQFFNTSALSISVIAVLEFEIPIDEAKIMSYAKDLIPLNPLFS 61

Query: 63  SIMVDDINGERKWKKVEVNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKN 122
           SIMV+DINGERKWKKVEV +EE IL+   PSNLS   +  YDAYFN+YIT+L  QEL +N
Sbjct: 62  SIMVNDINGERKWKKVEVKVEEQILVAAPPSNLS---NELYDAYFNEYITDLSVQELPQN 121

Query: 123 KPLWEIHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSRKIN 182
           KPLWEIHI+  PTSNAA+N+ILKFHHSLGDGYSVMGLL+SSM RADNPSLPLTFPS KIN
Sbjct: 122 KPLWEIHILNCPTSNAASNIILKFHHSLGDGYSVMGLLLSSMTRADNPSLPLTFPSSKIN 181

Query: 183 SDDHNNKNLRHVLNYVPSFMNSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTI 242
           S+  + +    V   + S +NSVLDFG S+IKSSVLED+LTPIRSG DGVEF+P +I T+
Sbjct: 182 SNQ-SRQIPSGVSQVLLSSINSVLDFGLSLIKSSVLEDELTPIRSGDDGVEFKPTSIWTM 241

Query: 243 SFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYMHETCPDSIQSNSTTIVLLNTRMFGNY 302
           +FSLHQIKQIKSKLRVTINDVI G++FLGIRLYM ET PDS +SNST +VLLNTRMFG Y
Sbjct: 242 NFSLHQIKQIKSKLRVTINDVIAGVLFLGIRLYMEETRPDSTKSNSTALVLLNTRMFGTY 301

Query: 303 KSVEDMLKPNSNAPWGNRLALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLL 362
           K +EDML PNSN PWGNR A L IDIPKLTDYELSNP+QF++AAQKLIKRKR S A FLL
Sbjct: 302 KCMEDMLNPNSNTPWGNRFAFLHIDIPKLTDYELSNPLQFVEAAQKLIKRKRDSSAVFLL 361

Query: 363 DKLMEMVHKLKGPEVAAKYMYKMVRNSSLSISNMIGPKEKMALLGHPAKGVYFILFGIPQ 422
           DKLMEM+HK +G E AAKY+YK ++NSS+SISN+IGPKEK+AL GHPAKG+YF + GIPQ
Sbjct: 362 DKLMEMIHKFRGSEAAAKYVYKTIKNSSISISNLIGPKEKIALSGHPAKGMYFTVVGIPQ 421

Query: 423 SLIITMVSYMENLRIAFGSEKEFIDQEKLTSCMKKAFEHMYKAASVDVSI 472
           SL IT++SYMENLRIAFGSEK+FIDQEK T CMKKAF+H+++A S+ VSI
Sbjct: 422 SLKITVMSYMENLRIAFGSEKDFIDQEKFTCCMKKAFDHIHEA-SIYVSI 466

BLAST of Cucsat.G9343 vs. NCBI nr
Match: XP_004136846.2 (O-acyltransferase WSD1 [Cucumis sativus] >KGN43650.1 hypothetical protein Csa_017285 [Cucumis sativus])

HSP 1 Score: 662 bits (1708), Expect = 2.53e-235
Identity = 340/470 (72.34%), Postives = 398/470 (84.68%), Query Frame = 0

Query: 7   EEEEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMV 66
           EEE+E LEP+SPTAQC N+S+LSISVIAVLEFEIPIDE +I+CYAKD IPLN LFSSI V
Sbjct: 2   EEEDEFLEPMSPTAQCLNTSSLSISVIAVLEFEIPIDEAKIICYAKDFIPLNSLFSSITV 61

Query: 67  DDINGERKWKKVEVNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPLW 126
           +DINGERKWKKVEV +EE I++ T PS+LS+  ++N DA+FN+YIT L  QEL +NKPLW
Sbjct: 62  NDINGERKWKKVEVKVEEQIVVATPPSDLSI--ELN-DAFFNEYITKLSVQELDQNKPLW 121

Query: 127 EIHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSRKINSDDH 186
           EIHI+  PTS AAANLILKFHHSLGDGYSVMGLL+S+MKRADNPSLPLTFP    N    
Sbjct: 122 EIHILNCPTSTAAANLILKFHHSLGDGYSVMGLLLSTMKRADNPSLPLTFP---FNGKMI 181

Query: 187 NNKNLRHVLNYVPSFM----NSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTI 246
           N+K L  + + V  F+    NSVLDFG S+IKSSVLED+LTPIRSG DGVEF+P  I TI
Sbjct: 182 NSKKLGQISSGVSHFLLSSINSVLDFGLSLIKSSVLEDELTPIRSGGDGVEFKPTEIWTI 241

Query: 247 SFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYMHETCPDSIQSNSTTIVLLNTRMFGNY 306
           +FSLHQIKQIKSKLRVT+NDV+ G++FLGIRLYM ET PDS +SNST +VLLNTRMFG Y
Sbjct: 242 TFSLHQIKQIKSKLRVTVNDVVAGMLFLGIRLYMEETHPDSTKSNSTALVLLNTRMFGTY 301

Query: 307 KSVEDMLKPNSNAPWGNRLALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLL 366
           K +EDML PNSN PWGNR   L IDIPKLTD+ LSNP+QF++AAQKLIKRKR S A FL+
Sbjct: 302 KCMEDMLNPNSNTPWGNRFGFLHIDIPKLTDFNLSNPLQFVQAAQKLIKRKRDSSAVFLV 361

Query: 367 DKLMEMVHKLKGPEVAAKYMYKMVRNSSLSISNMIGPKEKMALLGHPAKGVYFILFGIPQ 426
           DKLME++HK +G EVA+KY+YK ++NSSLSI+N+IGPKEKM L GHP KG+YF + GIPQ
Sbjct: 362 DKLMEIIHKFRGSEVASKYVYKTIKNSSLSITNLIGPKEKMTLTGHPVKGMYFTVVGIPQ 421

Query: 427 SLIITMVSYMENLRIAFGSEKEFIDQEKLTSCMKKAFEHMYKAASVDVSI 472
           SL I+++SYMENLRIAFG EKEFIDQEKLTSCMKKAF+H++KA S+DVSI
Sbjct: 422 SLKISVISYMENLRIAFGGEKEFIDQEKLTSCMKKAFDHIHKA-SIDVSI 464

BLAST of Cucsat.G9343 vs. NCBI nr
Match: XP_016901702.1 (PREDICTED: O-acyltransferase WSD1-like, partial [Cucumis melo])

HSP 1 Score: 665 bits (1715), Expect = 4.78e-233
Identity = 339/466 (72.75%), Postives = 403/466 (86.48%), Query Frame = 0

Query: 7   EEEEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMV 66
           EEE+ELLEPVSPTAQCFN+SALSISVIAV+EFEIPIDE +IMCYAKD IPLNPLFSSIMV
Sbjct: 2   EEEDELLEPVSPTAQCFNTSALSISVIAVVEFEIPIDEAKIMCYAKDFIPLNPLFSSIMV 61

Query: 67  DDINGERKWKKVEVNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPLW 126
           +DINGERKWKKVEV +EE I++ T PSNLS+  ++N + YFN+YIT L  +E A+NKPLW
Sbjct: 62  NDINGERKWKKVEVKVEEQIVVATPPSNLSI--ELN-NVYFNEYITKLSVKEFAQNKPLW 121

Query: 127 EIHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFP-SRKINSDD 186
           EIHI+  PTS+AAANL+LKFHHSLGDGYSVMGLL+SSMKRADNPSLPLTFP + KI + +
Sbjct: 122 EIHILNCPTSDAAANLVLKFHHSLGDGYSVMGLLLSSMKRADNPSLPLTFPFTGKIINSN 181

Query: 187 HNNKNLRHVLNYVPSFMNSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTISFS 246
              +    V +++ S +N+VLDFG S+IKSSVLED+LTPIRSG DGVEF+P  I TI+FS
Sbjct: 182 KFGQISSGVSHFLLSSINTVLDFGLSLIKSSVLEDELTPIRSGGDGVEFKPTEIWTITFS 241

Query: 247 LHQIKQIKSKLRVTINDVITGIIFLGIRLYMHETCPDSIQSNSTTIVLLNTRMFGNYKSV 306
           LH IKQIKSKLR+T+NDV+ G++FLGIRLYM ET P+S +SNST +VLLNTRMF  YK +
Sbjct: 242 LHHIKQIKSKLRMTVNDVVVGMLFLGIRLYMEETRPESTKSNSTALVLLNTRMFRTYKCM 301

Query: 307 EDMLKPNSNAPWGNRLALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLDKL 366
           EDML PNSN PWGNR A L IDIPKLTD+ LSNP+Q ++AAQKLIKRKR S A FL+DKL
Sbjct: 302 EDMLNPNSNTPWGNRFAFLHIDIPKLTDFNLSNPLQLVEAAQKLIKRKRDSSAVFLVDKL 361

Query: 367 MEMVHKLKGPEVAAKYMYKMVRNSSLSISNMIGPKEKMALLGHPAKGVYFILFGIPQSLI 426
           M+M+HK +G EVA+KY+YK ++NSSLSISN+IGPKEKMAL GHPAKG+YF + GIPQSL 
Sbjct: 362 MQMIHKFRGSEVASKYVYKTIKNSSLSISNLIGPKEKMALTGHPAKGMYFTVVGIPQSLK 421

Query: 427 ITMVSYMENLRIAFGSEKEFIDQEKLTSCMKKAFEHMYKAASVDVS 471
           I+++SYMENLRIAFG EKEFIDQEKLTSCMKKAF++++KA S+DVS
Sbjct: 422 ISVISYMENLRIAFGGEKEFIDQEKLTSCMKKAFDYIHKA-SIDVS 463

BLAST of Cucsat.G9343 vs. NCBI nr
Match: XP_022969083.1 (O-acyltransferase WSD1-like [Cucurbita maxima])

HSP 1 Score: 638 bits (1646), Expect = 6.78e-226
Identity = 326/465 (70.11%), Postives = 392/465 (84.30%), Query Frame = 0

Query: 9   EEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMVDD 68
           E+ELL+PVSP AQ  N S  SIS+IAVLEF+I IDE++ MC+AKDLI LNPLF+S+MV+D
Sbjct: 5   EDELLQPVSPNAQHMNCSVQSISIIAVLEFDIAIDELKFMCFAKDLIHLNPLFTSVMVND 64

Query: 69  INGERKWKKVEVNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPLWEI 128
           +NGERKWKKVE+ +EEHI++ T PSNLS+     +DAYFN+Y+TN   QEL++NKPLWEI
Sbjct: 65  VNGERKWKKVELKIEEHIIVHTPPSNLSLQ---LFDAYFNEYMTNYALQELSQNKPLWEI 124

Query: 129 HIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSRKINSDDHNN 188
           HII  PTSNAA NL+ K HHSLGDGYS+MG+LISSM RA +PSLPLTFPSRK+  +   +
Sbjct: 125 HIINCPTSNAAGNLVFKIHHSLGDGYSLMGVLISSMTRAHDPSLPLTFPSRKVKLEQSGH 184

Query: 189 KNLRHVLNYVPSFMNSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQ 248
             L  V  ++ S MN+VLDFG +I+KSSVLED LTPIRSG DGVEFR  AI T +FSLHQ
Sbjct: 185 I-LGRVYQFLFSSMNTVLDFGGNIMKSSVLEDHLTPIRSGGDGVEFRTFAIRTFTFSLHQ 244

Query: 249 IKQIKSKLRVTINDVITGIIFLGIRLYMHETCPDSIQSNSTTIVLLNTRMFGNYKSVEDM 308
           IKQIK+KL VT+NDVITG+IFLG+RLYM ETCPDS  SNST +VL+NTRM  NYKSV+DM
Sbjct: 245 IKQIKAKLGVTVNDVITGMIFLGLRLYMQETCPDSTNSNSTALVLINTRMVENYKSVQDM 304

Query: 309 LKPN-SNAPWGNRLALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLME 368
           LKPN SNAPWGNR A L IDIPK T+YELSNP+QFIKAAQK+IKRKRYS A FL+ KLME
Sbjct: 305 LKPNNSNAPWGNRFAFLHIDIPKATEYELSNPLQFIKAAQKMIKRKRYSSAVFLVGKLME 364

Query: 369 MVHKLKGPEVAAKYMYKMVRNSSLSISNMIGPKEKMALLGHPAKGVYFILFGIPQSLIIT 428
           M+HKL+GPEVAAK+MYK  +N+SLSISNM+GP+EKMA+ GHP KGVYF + GIPQSL+IT
Sbjct: 365 MIHKLRGPEVAAKFMYKTAQNTSLSISNMMGPREKMAMCGHPVKGVYFTVVGIPQSLMIT 424

Query: 429 MVSYMENLRIAFGSEKEFIDQEKLTSCMKKAFEHMYKAASVDVSI 472
           ++SYME LRI   SEKEFID+EKLTSCM+KAFE++++A S+++SI
Sbjct: 425 VISYMEQLRITILSEKEFIDEEKLTSCMEKAFENIHEA-SMELSI 464

BLAST of Cucsat.G9343 vs. ExPASy TrEMBL
Match: A0A0A0K1W0 (Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051300 PE=4 SV=1)

HSP 1 Score: 662 bits (1708), Expect = 1.22e-235
Identity = 340/470 (72.34%), Postives = 398/470 (84.68%), Query Frame = 0

Query: 7   EEEEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMV 66
           EEE+E LEP+SPTAQC N+S+LSISVIAVLEFEIPIDE +I+CYAKD IPLN LFSSI V
Sbjct: 2   EEEDEFLEPMSPTAQCLNTSSLSISVIAVLEFEIPIDEAKIICYAKDFIPLNSLFSSITV 61

Query: 67  DDINGERKWKKVEVNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPLW 126
           +DINGERKWKKVEV +EE I++ T PS+LS+  ++N DA+FN+YIT L  QEL +NKPLW
Sbjct: 62  NDINGERKWKKVEVKVEEQIVVATPPSDLSI--ELN-DAFFNEYITKLSVQELDQNKPLW 121

Query: 127 EIHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSRKINSDDH 186
           EIHI+  PTS AAANLILKFHHSLGDGYSVMGLL+S+MKRADNPSLPLTFP    N    
Sbjct: 122 EIHILNCPTSTAAANLILKFHHSLGDGYSVMGLLLSTMKRADNPSLPLTFP---FNGKMI 181

Query: 187 NNKNLRHVLNYVPSFM----NSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTI 246
           N+K L  + + V  F+    NSVLDFG S+IKSSVLED+LTPIRSG DGVEF+P  I TI
Sbjct: 182 NSKKLGQISSGVSHFLLSSINSVLDFGLSLIKSSVLEDELTPIRSGGDGVEFKPTEIWTI 241

Query: 247 SFSLHQIKQIKSKLRVTINDVITGIIFLGIRLYMHETCPDSIQSNSTTIVLLNTRMFGNY 306
           +FSLHQIKQIKSKLRVT+NDV+ G++FLGIRLYM ET PDS +SNST +VLLNTRMFG Y
Sbjct: 242 TFSLHQIKQIKSKLRVTVNDVVAGMLFLGIRLYMEETHPDSTKSNSTALVLLNTRMFGTY 301

Query: 307 KSVEDMLKPNSNAPWGNRLALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLL 366
           K +EDML PNSN PWGNR   L IDIPKLTD+ LSNP+QF++AAQKLIKRKR S A FL+
Sbjct: 302 KCMEDMLNPNSNTPWGNRFGFLHIDIPKLTDFNLSNPLQFVQAAQKLIKRKRDSSAVFLV 361

Query: 367 DKLMEMVHKLKGPEVAAKYMYKMVRNSSLSISNMIGPKEKMALLGHPAKGVYFILFGIPQ 426
           DKLME++HK +G EVA+KY+YK ++NSSLSI+N+IGPKEKM L GHP KG+YF + GIPQ
Sbjct: 362 DKLMEIIHKFRGSEVASKYVYKTIKNSSLSITNLIGPKEKMTLTGHPVKGMYFTVVGIPQ 421

Query: 427 SLIITMVSYMENLRIAFGSEKEFIDQEKLTSCMKKAFEHMYKAASVDVSI 472
           SL I+++SYMENLRIAFG EKEFIDQEKLTSCMKKAF+H++KA S+DVSI
Sbjct: 422 SLKISVISYMENLRIAFGGEKEFIDQEKLTSCMKKAFDHIHKA-SIDVSI 464

BLAST of Cucsat.G9343 vs. ExPASy TrEMBL
Match: A0A1S4E0D6 (Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495689 PE=4 SV=1)

HSP 1 Score: 665 bits (1715), Expect = 2.32e-233
Identity = 339/466 (72.75%), Postives = 403/466 (86.48%), Query Frame = 0

Query: 7   EEEEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMV 66
           EEE+ELLEPVSPTAQCFN+SALSISVIAV+EFEIPIDE +IMCYAKD IPLNPLFSSIMV
Sbjct: 2   EEEDELLEPVSPTAQCFNTSALSISVIAVVEFEIPIDEAKIMCYAKDFIPLNPLFSSIMV 61

Query: 67  DDINGERKWKKVEVNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPLW 126
           +DINGERKWKKVEV +EE I++ T PSNLS+  ++N + YFN+YIT L  +E A+NKPLW
Sbjct: 62  NDINGERKWKKVEVKVEEQIVVATPPSNLSI--ELN-NVYFNEYITKLSVKEFAQNKPLW 121

Query: 127 EIHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFP-SRKINSDD 186
           EIHI+  PTS+AAANL+LKFHHSLGDGYSVMGLL+SSMKRADNPSLPLTFP + KI + +
Sbjct: 122 EIHILNCPTSDAAANLVLKFHHSLGDGYSVMGLLLSSMKRADNPSLPLTFPFTGKIINSN 181

Query: 187 HNNKNLRHVLNYVPSFMNSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTISFS 246
              +    V +++ S +N+VLDFG S+IKSSVLED+LTPIRSG DGVEF+P  I TI+FS
Sbjct: 182 KFGQISSGVSHFLLSSINTVLDFGLSLIKSSVLEDELTPIRSGGDGVEFKPTEIWTITFS 241

Query: 247 LHQIKQIKSKLRVTINDVITGIIFLGIRLYMHETCPDSIQSNSTTIVLLNTRMFGNYKSV 306
           LH IKQIKSKLR+T+NDV+ G++FLGIRLYM ET P+S +SNST +VLLNTRMF  YK +
Sbjct: 242 LHHIKQIKSKLRMTVNDVVVGMLFLGIRLYMEETRPESTKSNSTALVLLNTRMFRTYKCM 301

Query: 307 EDMLKPNSNAPWGNRLALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLDKL 366
           EDML PNSN PWGNR A L IDIPKLTD+ LSNP+Q ++AAQKLIKRKR S A FL+DKL
Sbjct: 302 EDMLNPNSNTPWGNRFAFLHIDIPKLTDFNLSNPLQLVEAAQKLIKRKRDSSAVFLVDKL 361

Query: 367 MEMVHKLKGPEVAAKYMYKMVRNSSLSISNMIGPKEKMALLGHPAKGVYFILFGIPQSLI 426
           M+M+HK +G EVA+KY+YK ++NSSLSISN+IGPKEKMAL GHPAKG+YF + GIPQSL 
Sbjct: 362 MQMIHKFRGSEVASKYVYKTIKNSSLSISNLIGPKEKMALTGHPAKGMYFTVVGIPQSLK 421

Query: 427 ITMVSYMENLRIAFGSEKEFIDQEKLTSCMKKAFEHMYKAASVDVS 471
           I+++SYMENLRIAFG EKEFIDQEKLTSCMKKAF++++KA S+DVS
Sbjct: 422 ISVISYMENLRIAFGGEKEFIDQEKLTSCMKKAFDYIHKA-SIDVS 463

BLAST of Cucsat.G9343 vs. ExPASy TrEMBL
Match: A0A6J1HZY6 (Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468185 PE=4 SV=1)

HSP 1 Score: 638 bits (1646), Expect = 3.28e-226
Identity = 326/465 (70.11%), Postives = 392/465 (84.30%), Query Frame = 0

Query: 9   EEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMVDD 68
           E+ELL+PVSP AQ  N S  SIS+IAVLEF+I IDE++ MC+AKDLI LNPLF+S+MV+D
Sbjct: 5   EDELLQPVSPNAQHMNCSVQSISIIAVLEFDIAIDELKFMCFAKDLIHLNPLFTSVMVND 64

Query: 69  INGERKWKKVEVNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPLWEI 128
           +NGERKWKKVE+ +EEHI++ T PSNLS+     +DAYFN+Y+TN   QEL++NKPLWEI
Sbjct: 65  VNGERKWKKVELKIEEHIIVHTPPSNLSLQ---LFDAYFNEYMTNYALQELSQNKPLWEI 124

Query: 129 HIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSRKINSDDHNN 188
           HII  PTSNAA NL+ K HHSLGDGYS+MG+LISSM RA +PSLPLTFPSRK+  +   +
Sbjct: 125 HIINCPTSNAAGNLVFKIHHSLGDGYSLMGVLISSMTRAHDPSLPLTFPSRKVKLEQSGH 184

Query: 189 KNLRHVLNYVPSFMNSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQ 248
             L  V  ++ S MN+VLDFG +I+KSSVLED LTPIRSG DGVEFR  AI T +FSLHQ
Sbjct: 185 I-LGRVYQFLFSSMNTVLDFGGNIMKSSVLEDHLTPIRSGGDGVEFRTFAIRTFTFSLHQ 244

Query: 249 IKQIKSKLRVTINDVITGIIFLGIRLYMHETCPDSIQSNSTTIVLLNTRMFGNYKSVEDM 308
           IKQIK+KL VT+NDVITG+IFLG+RLYM ETCPDS  SNST +VL+NTRM  NYKSV+DM
Sbjct: 245 IKQIKAKLGVTVNDVITGMIFLGLRLYMQETCPDSTNSNSTALVLINTRMVENYKSVQDM 304

Query: 309 LKPN-SNAPWGNRLALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLME 368
           LKPN SNAPWGNR A L IDIPK T+YELSNP+QFIKAAQK+IKRKRYS A FL+ KLME
Sbjct: 305 LKPNNSNAPWGNRFAFLHIDIPKATEYELSNPLQFIKAAQKMIKRKRYSSAVFLVGKLME 364

Query: 369 MVHKLKGPEVAAKYMYKMVRNSSLSISNMIGPKEKMALLGHPAKGVYFILFGIPQSLIIT 428
           M+HKL+GPEVAAK+MYK  +N+SLSISNM+GP+EKMA+ GHP KGVYF + GIPQSL+IT
Sbjct: 365 MIHKLRGPEVAAKFMYKTAQNTSLSISNMMGPREKMAMCGHPVKGVYFTVVGIPQSLMIT 424

Query: 429 MVSYMENLRIAFGSEKEFIDQEKLTSCMKKAFEHMYKAASVDVSI 472
           ++SYME LRI   SEKEFID+EKLTSCM+KAFE++++A S+++SI
Sbjct: 425 VISYMEQLRITILSEKEFIDEEKLTSCMEKAFENIHEA-SMELSI 464

BLAST of Cucsat.G9343 vs. ExPASy TrEMBL
Match: A0A6J1GLS6 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455511 PE=4 SV=1)

HSP 1 Score: 629 bits (1621), Expect = 2.08e-222
Identity = 327/465 (70.32%), Postives = 390/465 (83.87%), Query Frame = 0

Query: 9   EEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMVDD 68
           E+ELL+PVSPTAQ  NS   SIS+IAVLEFEI IDE + M +AKDLI LNPLF+SIMV+D
Sbjct: 5   EDELLQPVSPTAQSMNSFVRSISLIAVLEFEIAIDEHKFMSFAKDLIHLNPLFTSIMVND 64

Query: 69  INGERKWKKVEVNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPLWEI 128
           +NGERKWKKVEVN+EEHI++ T PSNLS+     +DAYFN+Y+TN   QEL +NKPLWEI
Sbjct: 65  VNGERKWKKVEVNIEEHIIVHTPPSNLSLQ---LFDAYFNEYMTNYALQELPQNKPLWEI 124

Query: 129 HIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSRKINSDDHNN 188
           HII  PT+NAA NL+ K HHSLGDGYS+MG+LISSM RA +PSLPLTFPSRK+  +   +
Sbjct: 125 HIINCPTNNAAGNLVFKIHHSLGDGYSLMGVLISSMTRAHDPSLPLTFPSRKVKLEQSGH 184

Query: 189 KNLRHVLNYVPSFMNSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQ 248
             L  V  ++ S MN+VLDFG +++KSSVLED LTPIRSG DGVEFR  AI TI+FSLH 
Sbjct: 185 I-LGRVYQFLLSSMNTVLDFGGNMMKSSVLEDHLTPIRSGGDGVEFRTFAIRTITFSLHH 244

Query: 249 IKQIKSKLRVTINDVITGIIFLGIRLYMHETCPDSIQSNSTTIVLLNTRMFGNYKSVEDM 308
           IK+IK+KL VTINDVITG+IFLG+RLYM ET PDS  SNST +VL+NTRM  NYKSV+DM
Sbjct: 245 IKKIKAKLGVTINDVITGMIFLGLRLYMQETRPDSTNSNSTALVLINTRMVENYKSVQDM 304

Query: 309 LKPN-SNAPWGNRLALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLME 368
           LKPN SNAPWGNR ALL IDIPK TD ELSNP+QFIKAAQK+IKRKRYS A FL+ KLME
Sbjct: 305 LKPNNSNAPWGNRFALLHIDIPKATDCELSNPLQFIKAAQKMIKRKRYSSAVFLVGKLME 364

Query: 369 MVHKLKGPEVAAKYMYKMVRNSSLSISNMIGPKEKMALLGHPAKGVYFILFGIPQSLIIT 428
           M+HKL+GPEVAAK+MYK  +N+SLSISNM+GP+EKMA+ GHP KGVYF + GIPQSL+IT
Sbjct: 365 MIHKLRGPEVAAKFMYKTAQNTSLSISNMMGPREKMAMCGHPVKGVYFTVVGIPQSLMIT 424

Query: 429 MVSYMENLRIAFGSEKEFIDQEKLTSCMKKAFEHMYKAASVDVSI 472
           ++SYME LRI   SEKEFID+EKLTSC++KAFE++++A S+++SI
Sbjct: 425 VISYMEQLRITLLSEKEFIDEEKLTSCIEKAFENIHEA-SMELSI 464

BLAST of Cucsat.G9343 vs. ExPASy TrEMBL
Match: A0A6J1GJA7 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454845 PE=4 SV=1)

HSP 1 Score: 615 bits (1587), Expect = 3.06e-217
Identity = 321/465 (69.03%), Postives = 379/465 (81.51%), Query Frame = 0

Query: 9   EEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMVDD 68
           E+ELL+PVSPTAQ  NSS  SIS+I VLE E+ IDE+Q M +AK  I LNPLF+SIMV+D
Sbjct: 5   EDELLQPVSPTAQHMNSSVQSISLIVVLELEMAIDELQFMSFAKHFIHLNPLFTSIMVND 64

Query: 69  INGERKWKKVEVNLEEHILLPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPLWEI 128
           +NGERKWK+VEVN+EEHI++ T PSNLS+     +DAYFN+Y+TN   QEL +NKPLWEI
Sbjct: 65  VNGERKWKEVEVNIEEHIIVHTPPSNLSLQ---LFDAYFNEYMTNYALQELPQNKPLWEI 124

Query: 129 HIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSRKINSDDHNN 188
           HII  PTSNAA NL+ K  HSLGDGYS MGLLIS M RA NPSLPLTFPSR I ++   +
Sbjct: 125 HIINCPTSNAAGNLVFKIRHSLGDGYSFMGLLISGMTRAHNPSLPLTFPSRTIKAEQSGH 184

Query: 189 KNLRHVLNYVPSFMNSVLDFGWSIIKSSVLEDDLTPIRSGVDGVEFRPVAICTISFSLHQ 248
             L  V  ++ S MN+VLDFGW+I+KSSVLED LTPIRSG D VEFR  AI TI+FSL Q
Sbjct: 185 I-LGRVSQFLLSSMNTVLDFGWNIMKSSVLEDHLTPIRSGRDDVEFRTFAIRTITFSLPQ 244

Query: 249 IKQIKSKLRVTINDVITGIIFLGIRLYMHETCPDSIQSNSTTIVLLNTRMFGNYKSVEDM 308
           IKQIK+KL  TINDVITG+IFLG+RLYM ET PDS  SNST +VL+NTRM GNYKS++DM
Sbjct: 245 IKQIKTKLGETINDVITGMIFLGLRLYMQETRPDSTNSNSTALVLINTRMIGNYKSLQDM 304

Query: 309 LKPN-SNAPWGNRLALLPIDIPKLTDYELSNPIQFIKAAQKLIKRKRYSYATFLLDKLME 368
           LK N SN  WGNR   L IDIPK T+YELSNP+QFIKAAQ++IKRKRYS A +L+DK+ME
Sbjct: 305 LKANNSNTSWGNRFGFLHIDIPKATEYELSNPLQFIKAAQEMIKRKRYSSAVYLVDKVME 364

Query: 369 MVHKLKGPEVAAKYMYKMVRNSSLSISNMIGPKEKMALLGHPAKGVYFILFGIPQSLIIT 428
           M+HKL+GPEVAAK MYK  RN+SL ISNMIGP+EKMA+ GHP KGVYF + G+ QSL+IT
Sbjct: 365 MIHKLRGPEVAAKLMYKTFRNTSLVISNMIGPREKMAVCGHPVKGVYFTVVGVAQSLVIT 424

Query: 429 MVSYMENLRIAFGSEKEFIDQEKLTSCMKKAFEHMYKAASVDVSI 472
           M+SYM++LRI F SEKEFID+ KLTSCM KAFE++YKA S++VSI
Sbjct: 425 MISYMDDLRITFRSEKEFIDEVKLTSCMNKAFENIYKA-SMEVSI 464