Cucsat.G8246.T1 (mRNA) Cucumber (B10) v3

BLAST of Cucsat.G8246.T1 vs. NCBI nr
Match: XP_011656281.2 (proline-rich protein 4 [Cucumis sativus] >KAE8648949.1 hypothetical protein Csa_008537 [Cucumis sativus])

HSP 1 Score: 620 bits (1599), Expect = 7.04e-224
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MSWLLILLLLNFSFFDLSEARHHRKLPSAVVVGTVFCDTCYQEKFSKTSHFISGATVAVE 60
           MSWLLILLLLNFSFFDLSEARHHRKLPSAVVVGTVFCDTCYQEKFSKTSHFISGATVAVE
Sbjct: 1   MSWLLILLLLNFSFFDLSEARHHRKLPSAVVVGTVFCDTCYQEKFSKTSHFISGATVAVE 60

Query: 61  CGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATAKS 120
           CGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATAKS
Sbjct: 61  CGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATAKS 120

Query: 121 SSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIQLPPPPSYDIPNL 180
           SSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIQLPPPPSYDIPNL
Sbjct: 121 SSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIQLPPPPSYDIPNL 180

Query: 181 PSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPKEK 240
           PSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPKEK
Sbjct: 181 PSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPKEK 240

Query: 241 DEKNAPNETPNTSEKLDKFPIPPIKPLRKPHHFVLPPQRLHHHPRLPPHVAVIGGEPIPN 300
           DEKNAPNETPNTSEKLDKFPIPPIKPLRKPHHFVLPPQRLHHHPRLPPHVAVIGGEPIPN
Sbjct: 241 DEKNAPNETPNTSEKLDKFPIPPIKPLRKPHHFVLPPQRLHHHPRLPPHVAVIGGEPIPN 300

Query: 301 LSKISSSHKKTSP 313
           LSKISSSHKKTSP
Sbjct: 301 LSKISSSHKKTSP 313

BLAST of Cucsat.G8246.T1 vs. NCBI nr
Match: KAA0039420.1 (major pollen allergen Lol p 11 [Cucumis melo var. makuwa] >TYK00608.1 major pollen allergen Lol p 11 [Cucumis melo var. makuwa])

HSP 1 Score: 563 bits (1450), Expect = 3.74e-201
Identity = 285/314 (90.76%), Postives = 298/314 (94.90%), Query Frame = 0

Query: 1   MSWLLILLLLNFSFFDLSEARHHRKLPSAVVVGTVFCDTCYQEKFSKTSHFISGATVAVE 60
           M WLLILLLLNFSFFDLSEARHHRKLPSAV++GTVFCDTC+QEKFSKTSHFISGATVAVE
Sbjct: 1   MIWLLILLLLNFSFFDLSEARHHRKLPSAVIIGTVFCDTCFQEKFSKTSHFISGATVAVE 60

Query: 61  CGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATAKS 120
           CGN+G +PSFREEVKTDKRGEFKVNLPVLVSKHV+KIEECYVE +KSSEPYCDVAATAKS
Sbjct: 61  CGNRGRKPSFREEVKTDKRGEFKVNLPVLVSKHVEKIEECYVESIKSSEPYCDVAATAKS 120

Query: 121 SSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEI-QLPPPPSYDIPN 180
           SSLQLKS+KQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEI QLPPPP +D PN
Sbjct: 121 SSLQLKSKKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIIQLPPPPPFDSPN 180

Query: 181 LPSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPKE 240
           LPSPIQ PTVP+APRIYDNLPPLPLLPGLLPLP LPPLPPLPPLP LPPLPKFPIFPPK 
Sbjct: 181 LPSPIQNPTVPNAPRIYDNLPPLPLLPGLLPLPPLPPLPPLPPLPPLPPLPKFPIFPPKA 240

Query: 241 KDEKNAPNETPNTSEKLDKFPIPPIKPLRKPHHFVLPPQRLHHHPRLPPHVAVIGGEPIP 300
            DEKNAP ETPNTSEKLDKFPIPPIKPLR+PH+FVLPPQRLHHHP+ PPHVAVIGGEPIP
Sbjct: 241 NDEKNAPIETPNTSEKLDKFPIPPIKPLRRPHYFVLPPQRLHHHPQPPPHVAVIGGEPIP 300

Query: 301 NLSKISSSHKKTSP 313
           NLS ISS  KKTSP
Sbjct: 301 NLSNISSPQKKTSP 314

BLAST of Cucsat.G8246.T1 vs. NCBI nr
Match: XP_038889683.1 (pollen-specific leucine-rich repeat extensin-like protein 3 [Benincasa hispida])

HSP 1 Score: 438 bits (1127), Expect = 5.71e-152
Identity = 239/318 (75.16%), Postives = 264/318 (83.02%), Query Frame = 0

Query: 1   MSWLLILLLLNFSFFDLSEARHHRKLPSAVVVGTVFCDTCYQEKFSKTSHFISGATVAVE 60
           M WL+ILLLLN SFFDLSEARHHRKLPSAVVVGTVFCDTC+QEKFSKTSHFISGATV V+
Sbjct: 5   MVWLIILLLLNLSFFDLSEARHHRKLPSAVVVGTVFCDTCFQEKFSKTSHFISGATVVVK 64

Query: 61  CGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATAKS 120
           CGN+G  PSFREEVKTDKRGEFKVNLPV VSKHVKKIEECYVEL+KSSEPYC VAATAKS
Sbjct: 65  CGNEGSRPSFREEVKTDKRGEFKVNLPVPVSKHVKKIEECYVELIKSSEPYCAVAATAKS 124

Query: 121 SSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIQLPPPPSYDIPNL 180
           SSLQLKS+KQ THTFSAGFFTFKPLKQPNLC Q+PQN NT+DD K++ L  PP++D P+L
Sbjct: 125 SSLQLKSKKQGTHTFSAGFFTFKPLKQPNLCKQRPQNSNTYDDTKQV-LAAPPTFDYPSL 184

Query: 181 PSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPKEK 240
           PSPIQ P VP   RIY+NLPPLPLLPGLLPLP   PLPPLPPLP LPPLP FP+FPPK K
Sbjct: 185 PSPIQNPNVP---RIYENLPPLPLLPGLLPLP---PLPPLPPLPPLPPLPGFPLFPPK-K 244

Query: 241 DEKNAPNETPNTSEKLDKFPIPPIKPLRKPHHFVLPPQRLHHHP----RLPPHVAVIG-G 300
           +E NAPNETPNTSEK ++F    + PLR+PH  VLPP RLHHH     RL P    +   
Sbjct: 245 NENNAPNETPNTSEKPNQFHPQTLLPLRRPHFSVLPPHRLHHHQPLLHRLQPAAGGVALS 304

Query: 301 EPIPNLSKISSSHKKTSP 313
            P+P+L +ISS  K+ SP
Sbjct: 305 LPLPDLPEISSPPKQNSP 314

BLAST of Cucsat.G8246.T1 vs. NCBI nr
Match: XP_023528636.1 (proline-rich protein 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 353 bits (905), Expect = 2.92e-118
Identity = 206/335 (61.49%), Postives = 233/335 (69.55%), Query Frame = 0

Query: 1   MSWLLILLLLNFSFFDLSEARHHRKLPSAVVVGTVFCDTCYQEKFSKTSHFISGATVAVE 60
           M  LLIL+ LNFS  DLS+ARHH  LPSA VVGTVFCDTC+Q+ FSKTSHFISGATVAVE
Sbjct: 1   MICLLILIALNFSLLDLSQARHHNNLPSAAVVGTVFCDTCFQDTFSKTSHFISGATVAVE 60

Query: 61  CGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATAKS 120
           CGN G  PSFR+EVKTDK GEFK+ LPV     V+KIEECYV L++SSEPYC VAA AKS
Sbjct: 61  CGNGGSNPSFRDEVKTDKTGEFKIQLPV----SVRKIEECYVRLIRSSEPYCAVAARAKS 120

Query: 121 SSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIQLPPPPSYDIPNL 180
           SSL+LKSRKQ  H FSAGFFTFKPLKQP LC+    + N FDD K++        D P L
Sbjct: 121 SSLKLKSRKQGMHVFSAGFFTFKPLKQPKLCSHN-SHFNEFDDTKQV-------VDFPGL 180

Query: 181 PSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPKEK 240
           P+PIQ PTVP+ PRIYDNLPPLPLLPGL PLPQLPPLPPLPPLP       FP+FPPK K
Sbjct: 181 PAPIQNPTVPNVPRIYDNLPPLPLLPGLPPLPQLPPLPPLPPLPV------FPLFPPK-K 240

Query: 241 DEKNAPNETPNTSEKLDKF------PIPPIKPLRKPHHFVLPPQRLHHHPRL----PPHV 300
           D++N   +TP  S+  D F      PIP +KP R   HFV+PP +L HHP      PP  
Sbjct: 241 DDENV--QTPKISQNPDLFHPQTLLPIPSLKPFRP--HFVMPPHKLRHHPLTHGPTPPSA 300

Query: 301 AVIGGE------------PIPNLSKISSSHKKTSP 313
           A   GE             IPN+ +ISS  K+TSP
Sbjct: 301 AAAAGELAPSPPLPFSLPSIPNMPEISSPPKQTSP 312

BLAST of Cucsat.G8246.T1 vs. NCBI nr
Match: XP_022990149.1 (amyloid beta A4 precursor protein-binding family B member 1-interacting protein-like [Cucurbita maxima])

HSP 1 Score: 343 bits (880), Expect = 1.39e-114
Identity = 199/316 (62.97%), Postives = 228/316 (72.15%), Query Frame = 0

Query: 1   MSWLLILLLLNFSFFDLSEARHHRKLPSAV--VVGTVFCDTCYQEKFSKTSHFISGATVA 60
           M  LLIL+ LNFSF DLS+ARHH  LPSA   VVGTVFCDTC+Q+ FSKTSHFISGATVA
Sbjct: 1   MICLLILIALNFSFLDLSQARHHNNLPSAAAAVVGTVFCDTCFQDTFSKTSHFISGATVA 60

Query: 61  VECGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATA 120
           VECGN+G  P+FREEVKTDK GEFK+ LPV     V+K+EECYV L++SSEPYC VAA A
Sbjct: 61  VECGNEGSNPNFREEVKTDKTGEFKIQLPV----SVRKVEECYVRLIRSSEPYCAVAARA 120

Query: 121 KSSSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIQLPPPPSYDIP 180
           KSSSL+LKS+KQ TH FSAGFFTFKPLKQP LC+    + N FDD K++        D P
Sbjct: 121 KSSSLRLKSKKQGTHVFSAGFFTFKPLKQPKLCSHNSHS-NEFDDTKQV-------VDFP 180

Query: 181 NLPSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPK 240
            LP+PIQ PTVP+ PRIYDNLPPLPLLPGL PLPQLPPLPPLPPLP       FP+FPPK
Sbjct: 181 GLPAPIQNPTVPNVPRIYDNLPPLPLLPGLPPLPQLPPLPPLPPLPI------FPLFPPK 240

Query: 241 EKDEKNAPNETPNTSEKLDKF------PIPPIKPLRKPHHFVLPPQRLHHHPRL----PP 300
            KD++N   +TP  S+  D F      PIP +KPLR   HFV+PP +L HHP      PP
Sbjct: 241 -KDDENV--QTPKISQNPDLFHPQTLLPIPSLKPLRP--HFVMPPHKLRHHPLTHGPTPP 293

BLAST of Cucsat.G8246.T1 vs. ExPASy TrEMBL
Match: A0A0A0KXM2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G642130 PE=4 SV=1)

HSP 1 Score: 572 bits (1475), Expect = 1.67e-205
Identity = 291/296 (98.31%), Postives = 291/296 (98.31%), Query Frame = 0

Query: 1   MSWLLILLLLNFSFFDLSEARHHRKLPSAVVVGTVFCDTCYQEKFSKTSHFISGATVAVE 60
           MSWLLILLLLNFSFFDLSEARHHRKLPSAVVVGTVFCDTCYQEKFSKTSHFISGATVAVE
Sbjct: 1   MSWLLILLLLNFSFFDLSEARHHRKLPSAVVVGTVFCDTCYQEKFSKTSHFISGATVAVE 60

Query: 61  CGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATAKS 120
           CGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATAKS
Sbjct: 61  CGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATAKS 120

Query: 121 SSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIQLPPPPSYDIPNL 180
           SSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIQLPPPPSYDIPNL
Sbjct: 121 SSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIQLPPPPSYDIPNL 180

Query: 181 PSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPKEK 240
           PSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPKEK
Sbjct: 181 PSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPKEK 240

Query: 241 DEKNAPNETPNTSEKLDKFPIPPIKPLRKPHHFVLPPQRLHHHPRLPPHVAVIGGE 296
           DEKNAPNETPNTSEKLDKFPIPPIKPLRKPHHFVLPPQRLHHHPRLPP     GGE
Sbjct: 241 DEKNAPNETPNTSEKLDKFPIPPIKPLRKPHHFVLPPQRLHHHPRLPPQSG--GGE 294

BLAST of Cucsat.G8246.T1 vs. ExPASy TrEMBL
Match: A0A5A7T850 (Major pollen allergen Lol p 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002260 PE=4 SV=1)

HSP 1 Score: 563 bits (1450), Expect = 1.81e-201
Identity = 285/314 (90.76%), Postives = 298/314 (94.90%), Query Frame = 0

Query: 1   MSWLLILLLLNFSFFDLSEARHHRKLPSAVVVGTVFCDTCYQEKFSKTSHFISGATVAVE 60
           M WLLILLLLNFSFFDLSEARHHRKLPSAV++GTVFCDTC+QEKFSKTSHFISGATVAVE
Sbjct: 1   MIWLLILLLLNFSFFDLSEARHHRKLPSAVIIGTVFCDTCFQEKFSKTSHFISGATVAVE 60

Query: 61  CGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATAKS 120
           CGN+G +PSFREEVKTDKRGEFKVNLPVLVSKHV+KIEECYVE +KSSEPYCDVAATAKS
Sbjct: 61  CGNRGRKPSFREEVKTDKRGEFKVNLPVLVSKHVEKIEECYVESIKSSEPYCDVAATAKS 120

Query: 121 SSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEI-QLPPPPSYDIPN 180
           SSLQLKS+KQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEI QLPPPP +D PN
Sbjct: 121 SSLQLKSKKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIIQLPPPPPFDSPN 180

Query: 181 LPSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPKE 240
           LPSPIQ PTVP+APRIYDNLPPLPLLPGLLPLP LPPLPPLPPLP LPPLPKFPIFPPK 
Sbjct: 181 LPSPIQNPTVPNAPRIYDNLPPLPLLPGLLPLPPLPPLPPLPPLPPLPPLPKFPIFPPKA 240

Query: 241 KDEKNAPNETPNTSEKLDKFPIPPIKPLRKPHHFVLPPQRLHHHPRLPPHVAVIGGEPIP 300
            DEKNAP ETPNTSEKLDKFPIPPIKPLR+PH+FVLPPQRLHHHP+ PPHVAVIGGEPIP
Sbjct: 241 NDEKNAPIETPNTSEKLDKFPIPPIKPLRRPHYFVLPPQRLHHHPQPPPHVAVIGGEPIP 300

Query: 301 NLSKISSSHKKTSP 313
           NLS ISS  KKTSP
Sbjct: 301 NLSNISSPQKKTSP 314

BLAST of Cucsat.G8246.T1 vs. ExPASy TrEMBL
Match: A0A6J1JRA2 (amyloid beta A4 precursor protein-binding family B member 1-interacting protein-like OS=Cucurbita maxima OX=3661 GN=LOC111487127 PE=4 SV=1)

HSP 1 Score: 343 bits (880), Expect = 6.71e-115
Identity = 199/316 (62.97%), Postives = 228/316 (72.15%), Query Frame = 0

Query: 1   MSWLLILLLLNFSFFDLSEARHHRKLPSAV--VVGTVFCDTCYQEKFSKTSHFISGATVA 60
           M  LLIL+ LNFSF DLS+ARHH  LPSA   VVGTVFCDTC+Q+ FSKTSHFISGATVA
Sbjct: 1   MICLLILIALNFSFLDLSQARHHNNLPSAAAAVVGTVFCDTCFQDTFSKTSHFISGATVA 60

Query: 61  VECGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATA 120
           VECGN+G  P+FREEVKTDK GEFK+ LPV     V+K+EECYV L++SSEPYC VAA A
Sbjct: 61  VECGNEGSNPNFREEVKTDKTGEFKIQLPV----SVRKVEECYVRLIRSSEPYCAVAARA 120

Query: 121 KSSSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIQLPPPPSYDIP 180
           KSSSL+LKS+KQ TH FSAGFFTFKPLKQP LC+    + N FDD K++        D P
Sbjct: 121 KSSSLRLKSKKQGTHVFSAGFFTFKPLKQPKLCSHNSHS-NEFDDTKQV-------VDFP 180

Query: 181 NLPSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPK 240
            LP+PIQ PTVP+ PRIYDNLPPLPLLPGL PLPQLPPLPPLPPLP       FP+FPPK
Sbjct: 181 GLPAPIQNPTVPNVPRIYDNLPPLPLLPGLPPLPQLPPLPPLPPLPI------FPLFPPK 240

Query: 241 EKDEKNAPNETPNTSEKLDKF------PIPPIKPLRKPHHFVLPPQRLHHHPRL----PP 300
            KD++N   +TP  S+  D F      PIP +KPLR   HFV+PP +L HHP      PP
Sbjct: 241 -KDDENV--QTPKISQNPDLFHPQTLLPIPSLKPLRP--HFVMPPHKLRHHPLTHGPTPP 293

BLAST of Cucsat.G8246.T1 vs. ExPASy TrEMBL
Match: A0A6J1HD47 (amyloid beta A4 precursor protein-binding family B member 1-interacting protein-like OS=Cucurbita moschata OX=3662 GN=LOC111463006 PE=4 SV=1)

HSP 1 Score: 338 bits (867), Expect = 1.05e-112
Identity = 202/342 (59.06%), Postives = 232/342 (67.84%), Query Frame = 0

Query: 1   MSWLLILLLLNFSFFDLSEARHHRKLPS-AVVVGTVFCDTCYQEKFSKTSHFISGATVAV 60
           M  LLIL+ LNFSF DLS+ARHH  LPS A VVGTVFCDTC+Q+ FSK+SHFISGATVAV
Sbjct: 1   MICLLILIALNFSFLDLSQARHHNNLPSTAAVVGTVFCDTCFQDTFSKSSHFISGATVAV 60

Query: 61  ECGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATAK 120
           ECG+ G  PSFR+EVKTDK GEFK+ LPV     V+KIEECYV L++SSEPYC VAA AK
Sbjct: 61  ECGDGGSNPSFRDEVKTDKTGEFKIQLPV----SVRKIEECYVRLIRSSEPYCAVAARAK 120

Query: 121 SSSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIQLPPPPSYDIPN 180
           SSSL+LKSRKQ TH FSAGFFTFKPLK P LC+    + + FDD K++        D P 
Sbjct: 121 SSSLKLKSRKQGTHVFSAGFFTFKPLKHPKLCSHNSHS-SEFDDTKQV-------VDFPG 180

Query: 181 LPSPIQIPTVPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPKE 240
           LP+PIQ PTVP+ PRIYDNLPPL LLPGL PLPQLPPLPPLPPLP       FP+FPPK 
Sbjct: 181 LPAPIQNPTVPNVPRIYDNLPPLTLLPGLPPLPQLPPLPPLPPLPV------FPLFPPK- 240

Query: 241 KDEKNAPNETPNTSEKLDKF------PIPPIKPLRKPHHFVLPPQRLHHHPRL------- 300
           KD++N   +TP  S+  D F      PIP +KPLR   HFV+PP +L HHP         
Sbjct: 241 KDDENV--QTPKISQNPDMFHPQTLLPIPSLKPLRP--HFVMPPHKLRHHPLTHGPFSPS 300

Query: 301 -----PPHVAV----------IGGEPIPNLSKISSSHKKTSP 313
                PP  A               PIP + +ISS  K+TSP
Sbjct: 301 FSTPTPPSAAADELAPSPPLPFSLPPIPRMPEISSPPKETSP 319

BLAST of Cucsat.G8246.T1 vs. ExPASy TrEMBL
Match: A0A6J1BYU3 (proline-rich protein 4-like OS=Momordica charantia OX=3673 GN=LOC111006489 PE=4 SV=1)

HSP 1 Score: 337 bits (863), Expect = 5.01e-112
Identity = 195/288 (67.71%), Postives = 213/288 (73.96%), Query Frame = 0

Query: 1   MSWLLILLLLNFSFFDLSEARHHRKLPSAVVVGTVFCDTCYQEKFSKTSHFISGATVAVE 60
           M WLLILL+LN SF DLS+ARHH+ LPSAV+VGTVFCDTC QEK SKTS FISGATVAVE
Sbjct: 1   MVWLLILLILNLSFCDLSQARHHKNLPSAVIVGTVFCDTCSQEKLSKTSRFISGATVAVE 60

Query: 61  CGNKGPEPSFREEVKTDKRGEFKVNLPVLVSKHVKKIEECYVELVKSSEPYCDVAATAKS 120
           CGN GP+PSFREEVKTDKRGEFKV+LPV VS+HVK IE C V L++SSE YC VAA A S
Sbjct: 61  CGNGGPKPSFREEVKTDKRGEFKVDLPVSVSRHVKTIEGCSVNLIRSSEAYCAVAAAATS 120

Query: 121 SSLQLKSRKQNTHTFSAGFFTFKPLKQPNLCNQKPQNPNTFDDMKEIQLPPPPSYDIPNL 180
           SS +LKSR Q TH FSAGFFTFKPLK PN+C QKP + NTF DMK+      P  D P L
Sbjct: 121 SSFELKSRNQGTHFFSAGFFTFKPLKHPNICTQKPYS-NTFHDMKQAL----PMLDYPAL 180

Query: 181 PSPIQIPT-VPSAPRIYDNLPPLPLLPGLLPLPQLPPLPPLPPLPTLPPLPKFPIFPPKE 240
           P+PI+ PT VP+ PRIYDNLPPLP LP L PLPQLPPLPPLPPLP       FPIFPPK 
Sbjct: 181 PTPIENPTIVPNVPRIYDNLPPLPFLPRLPPLPQLPPLPPLPPLPG------FPIFPPK- 240

Query: 241 KDEKNAPNETPNTSEKLDKFPIPPIKPLRKPHHFVLPPQRLHHHPRLP 287
           K  +NAPN          K  +P  K LR   HFVLPP RL H P  P
Sbjct: 241 KTVENAPN---------GKTLLPHKKHLRP--HFVLPPHRLQHPPLFP 265