Cucsat.G8212.T1 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G8212.T1
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionProtein disulfide isomerase (PDI)-like protein 4
Locationctg1557: 2936569 .. 2939341 (+)
RNA-Seq ExpressionCucsat.G8212.T1
SyntenyCucsat.G8212.T1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTATTGACTTAAAAGAGTATCATTACATTTTTCACATTCTTAATAATAATCCATAAAAGTTAATTTCTTTCTCACTAAGTTTGTGTTTCTCTTATTAATAACTGAGTTCTTTCATTTTCTTTTTCCATAACCCAACAATATAAACCTAAAGAATCATTTCTTTAATTATTATGGCTTTTGATGCTGTTCATGATCTCACTTCCCTCCTTTCCTCTGACAGGAGGGATTTTCTCATCCGTAACAATGGCGATCAGGTATATATCGTGCTATCTTTACAAAAAATGTTGTTATACACGTGATTATGATCGATCGATGACTTGTGTTTATTATTTTGGGTTATTTGTGTGTGTTATGAACAGGTGAAAATCAGTTCTTTAATTGGAAAGAGAGTGGGATTATATTTCTCAGCAGGATGGTGCCCTCCATGTCGACTTTTCACACCAAAGTTATCAGAATTTTACAAGGAACTGTTGGCTAATAAATCTAAAAATAATGACTTTGAAATTATCTTCATTTCTTCTGATAGAGATGCACTCTCATTCAAAGCTTACTTCTCAAAAATGCCATGGTTGGCCATTCCATTTGATGATTTAGAGACCCAAAAAAAGTTGAAAATATTGTTTCAACTTAGTTCAATTCCTTATCTTGTTGTTATTGATGGCAATGGCAAAGTTTCAAGTGCTGATGGTGTGAATTTAGTGAAGGAATTTGGAGTTGATGCTTACCCTTTTACTATTGATCGAAAAAAGCAACTTCTGGCTCAAAAAGAAGAGGCTAAGAAAAATAATCAAACCATTACTTCCGTTTTGGCTTCTGCTTCTCGCAATTATTTGGTTTCTAATGATGGAAAACAGGTATCTTTATATCTATGATCTTCTTCTTCTTTCTTGCTTTCACGGTTTAAACGGTGATCTTGTTGGTTTCGAGTTTTAAAATCACTTTTGTCATGTTTAATATCAAAAGAGCAAAGCCAAACGATGATTAAGTTGATAGTTGTTTGGTTTCATAAAATTTTAAAAATGAAGCTTAATCAATTATGATCATTACGTTCTACTTATAAATTTTTTTATCTACTTAGTCAAATTTTGAAAACTAAAAAGGAAAAATAGTCGTTTTGAAAATGGTTGATTTTGTTTTTAAAATGCCACTGAGAATTCTTGTGTTCCCTTATATCGTAAAGAGTTATGTGTAAATTCTAAATTTCACATAAAACTATATAACCATCTAATCATATTATTATACCAAACCTATAACCTAAATGTTGATATTTTGGAGTAATTTTGAACACCATGAACAGATTTCATCCATTTCAAAATTATTTTCCTAAACGCACCTTTCAAAATTAATTGTTACAACTTCAAATGGATATGTTAACCAAATTATTACAAACCTTACAAAATTTAGGAGCAAAAAGTTTTATTTTCAATGAAAGTAGAACTAAATCAAAATCGTTCTTAAAAATTGATAAAGAATAATTAGTACTTTATCGTAATCTATCACTACCTATATACTAACTAATAGCGATAACAATGTCCGATATTTTTTTGTATTTATAAATATTTTCAACTTTTGAATCTCAATGTCATTATACTATTTCTGTTGTACGTAGATTCCTGTCTCTGAGCTGGAAGGAAAATTGATAGGATTGTATTTCTCACTGCCTGGCCATGAACATTGTGATGCTTTTACACCAAAGTTAAGTGAAGTTTACAACAACTTGAAGAAGAAAAAAGAGAATTTTGAGATTGTGTTCGTCTCTTTAGAAGAAGAAGATGAAGATTTCTTCAATGAAGCATTCAAATCAATGCCATGGTTGGCATTGCCTTTTAAGGATGAGAAATGTCAAAAACTAAAGCTTTATTTTGATGTTGATGATATTCCTGCTCTTGTTATAACTGGCCAGGATGGGAGGACTTTAAACCCGAACGCAGTCGACCTGATCAAACAGCACGGAATTGATGCCTACCCTTTCACTCCCAAGAAACACGACGTAGTTCATGGCAAGGTAGAAGCTTCTTGTTGTGGTTGTGATGGGTCGAAGACGAGGGAGGAAACCAAAGATGAGAAGGTAGAAGTTTCTTGTGGCTCGATGGAGGAAGCCAAAGATGGGAAGGCAGAAGTTTCTTGTGGCTCGATGGAGGAAACCAAAGATGGGAAGGCAGAAGTTTCTTGTGGCTCGATGGAGGAAACCAAAGATGGGAAGGCAGAAGTTTCTTGTGGCTGCGATGGGTCGAAAAACGACGATGATGAAAAGATGGAGGAAACCAAAGATAAGATGGTAGAAGTTTCTTGTGGCTGCGATGGGTCGTCGAAAAATGACGATGACGAAAAGATGGAGGAAACCTGCAAAGATGGGAAGGTAGAAGTTTCTTGTTGTGGCTGTGATGAATCGAAAAACGATGGTGACCAAAAGATGGAGGAAACCAAGGAGGAGATCAAAGAAGGGTGTGGGTGCAAAGGATAAATAGCATATTCATTAACACAGCAAAGAATATGGAAGTTTATGAGTACACACATATACACACCCATAAGACTTTTATATAATCTGCTTCATTTGCTGCCTTTTTTCTTAAGACTTTGTTGTTTGAAATTGAATAAATCAAAGGAGGCTTGTAGTTTTAAGCATATATTTCAAGACATTTTTTGGTTTAGTTGTTCTAATTTGATGGCTTGAGCTATAACTCTCTTAGCTTGTATGTGATTTCTATGGTTAAAATGTTATTCTCTATAATATTTGTGAGTGTTATTAGTTTGTTTCTTACCTTACATT

Coding sequence (CDS)

ATGGCTTTTGATGCTGTTCATGATCTCACTTCCCTCCTTTCCTCTGACAGGAGGGATTTTCTCATCCGTAACAATGGCGATCAGGTGAAAATCAGTTCTTTAATTGGAAAGAGAGTGGGATTATATTTCTCAGCAGGATGGTGCCCTCCATGTCGACTTTTCACACCAAAGTTATCAGAATTTTACAAGGAACTGTTGGCTAATAAATCTAAAAATAATGACTTTGAAATTATCTTCATTTCTTCTGATAGAGATGCACTCTCATTCAAAGCTTACTTCTCAAAAATGCCATGGTTGGCCATTCCATTTGATGATTTAGAGACCCAAAAAAAGTTGAAAATATTGTTTCAACTTAGTTCAATTCCTTATCTTGTTGTTATTGATGGCAATGGCAAAGTTTCAAGTGCTGATGGTGTGAATTTAGTGAAGGAATTTGGAGTTGATGCTTACCCTTTTACTATTGATCGAAAAAAGCAACTTCTGGCTCAAAAAGAAGAGGCTAAGAAAAATAATCAAACCATTACTTCCGTTTTGGCTTCTGCTTCTCGCAATTATTTGGTTTCTAATGATGGAAAACAGATTCCTGTCTCTGAGCTGGAAGGAAAATTGATAGGATTGTATTTCTCACTGCCTGGCCATGAACATTGTGATGCTTTTACACCAAAGTTAAGTGAAGTTTACAACAACTTGAAGAAGAAAAAAGAGAATTTTGAGATTGTGTTCGTCTCTTTAGAAGAAGAAGATGAAGATTTCTTCAATGAAGCATTCAAATCAATGCCATGGTTGGCATTGCCTTTTAAGGATGAGAAATGTCAAAAACTAAAGCTTTATTTTGATGTTGATGATATTCCTGCTCTTGTTATAACTGGCCAGGATGGGAGGACTTTAAACCCGAACGCAGTCGACCTGATCAAACAGCACGGAATTGATGCCTACCCTTTCACTCCCAAGAAACACGACGTAGTTCATGGCAAGGTAGAAGCTTCTTGTTGTGGTTGTGATGGGTCGAAGACGAGGGAGGAAACCAAAGATGAGAAGGTAGAAGTTTCTTGTGGCTCGATGGAGGAAGCCAAAGATGGGAAGGCAGAAGTTTCTTGTGGCTCGATGGAGGAAACCAAAGATGGGAAGGCAGAAGTTTCTTGTGGCTCGATGGAGGAAACCAAAGATGGGAAGGCAGAAGTTTCTTGTGGCTGCGATGGGTCGAAAAACGACGATGATGAAAAGATGGAGGAAACCAAAGATAAGATGGTAGAAGTTTCTTGTGGCTGCGATGGGTCGTCGAAAAATGACGATGACGAAAAGATGGAGGAAACCTGCAAAGATGGGAAGGTAGAAGTTTCTTGTTGTGGCTGTGATGAATCGAAAAACGATGGTGACCAAAAGATGGAGGAAACCAAGGAGGAGATCAAAGAAGGGTGTGGGTGCAAAGGATAA

Protein sequence

MAFDAVHDLTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSEFYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSSIPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLASASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIVFVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNAVDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSKTREETKDEKVEVSCGSMEEAKDGKAEVSCGSMEETKDGKAEVSCGSMEETKDGKAEVSCGCDGSKNDDDEKMEETKDKMVEVSCGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEETKEEIKEGCGCKG
Homology
BLAST of Cucsat.G8212.T1 vs. ExPASy Swiss-Prot
Match: O80763 (Probable nucleoredoxin 1 OS=Arabidopsis thaliana OX=3702 GN=At1g60420 PE=1 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 2.2e-90
Identity = 164/329 (49.85%), Postives = 227/329 (69.00%), Query Frame = 0

Query: 4   DAVHDLTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSEFYK 63
           D   DL SLLSS  RDFL+RN+G+QVK+ SL+GK++GLYFSA WC PC+ FTP+L E Y 
Sbjct: 11  DDAQDLHSLLSSPARDFLVRNDGEQVKVDSLLGKKIGLYFSAAWCGPCQRFTPQLVEVYN 70

Query: 64  ELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSSIPY 123
           EL    S    FEI+F+S D D  SF  YF KMPWLA+PF D ET+ +L  LF++  IP 
Sbjct: 71  EL----SSKVGFEIVFVSGDEDEESFGDYFRKMPWLAVPFTDSETRDRLDELFKVRGIPN 130

Query: 124 LVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLASASR 183
           LV++D +GK+ + +GV +++ +G DAYPFT ++ K++   ++ A++  QT+ SVL + SR
Sbjct: 131 LVMVDDHGKLVNENGVGVIRSYGADAYPFTPEKMKEIKEDEDRARR-GQTLRSVLVTPSR 190

Query: 184 NYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIVFVS 243
           ++++S DG ++PVSELEGK IGL FS+  +  C   TPKL E Y  LK+ KE+FEIV +S
Sbjct: 191 DFVISPDGNKVPVSELEGKTIGLLFSVASYRKCTELTPKLVEFYTKLKENKEDFEIVLIS 250

Query: 244 LEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNAVDL 303
           L E+DE+ FN+ FK+ PWLALPF D+   KL  +F +  +P LVI G DG+T + N  + 
Sbjct: 251 L-EDDEESFNQDFKTKPWLALPFNDKSGSKLARHFMLSTLPTLVILGPDGKTRHSNVAEA 310

Query: 304 IKQHGIDAYPFTPKK----HDVVHGKVEA 329
           I  +G+ AYPFTP+K     ++   KVEA
Sbjct: 311 IDDYGVLAYPFTPEKFQELKELEKAKVEA 333

BLAST of Cucsat.G8212.T1 vs. ExPASy Swiss-Prot
Match: Q7Y0F2 (Probable nucleoredoxin 1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405900 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 4.0e-84
Identity = 154/323 (47.68%), Postives = 223/323 (69.04%), Query Frame = 0

Query: 10  TSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSEFYKELLANK 69
           T L S+D RDFL+RN+ D+VKISS+    V LYFSA WCPPCR FTPKL E Y EL+   
Sbjct: 15  TILASADGRDFLLRNSADKVKISSIKASTVALYFSASWCPPCRRFTPKLIEAYNELV--- 74

Query: 70  SKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSSIPYLVVIDG 129
           S+   FE++F+S D D  +F AYF+KMPWLA+PF D E   KL   +++  IP+LV++D 
Sbjct: 75  SQGKSFEVVFVSGDSDQDAFNAYFAKMPWLAVPFSDSEALAKLNERYKVMGIPHLVILDA 134

Query: 130 -NGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLASASRNYLVS 189
            +G++ + DGV LV E+G +AYPFT +R  + L ++E+A K+NQTI S+  + +R+YL++
Sbjct: 135 KSGEIYTEDGVELVHEYGTEAYPFTTERINE-LKEQEKAAKDNQTIHSLFGTPTRDYLIT 194

Query: 190 NDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIVFVSLEEED 249
           N G ++P+S+LEGK +GL F + G+     FT  L+++Y  LK   E FE+V VSL + D
Sbjct: 195 NKGDKVPISDLEGKYVGLCFVVNGYGPVVQFTSVLAKIYEKLKAVGEKFEVVMVSL-DGD 254

Query: 250 EDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNAVDLIKQHG 309
           E+ FNE+F  MPWLA+P  D+ C+KL  YF++  +P LV+ G DG+TLN +  D+I +HG
Sbjct: 255 EESFNESFADMPWLAIPQGDKMCEKLARYFELSGLPMLVLIGPDGKTLNDDIADIIDEHG 314

Query: 310 IDA---YPFTPKKHDVVHGKVEA 329
            DA   +PF+ +K +++  K +A
Sbjct: 315 PDAWEGFPFSAEKLEILAEKAKA 332

BLAST of Cucsat.G8212.T1 vs. ExPASy Swiss-Prot
Match: Q7Y0E8 (Probable nucleoredoxin 1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405500 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 8.9e-84
Identity = 155/324 (47.84%), Postives = 226/324 (69.75%), Query Frame = 0

Query: 9   LTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSEFYKELLAN 68
           + ++L++D RDFL+RN+ DQVKISS+    V LYFSA WCPPCR FTPKL E Y EL+  
Sbjct: 7   IATVLAADGRDFLLRNSADQVKISSIEASTVALYFSASWCPPCRRFTPKLIEAYNELV-- 66

Query: 69  KSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSSIPYLVVID 128
            S+  +FE++F+S D+D  +F AYF+KMPWLA+PF D E + KL   F++  IP+LV+++
Sbjct: 67  -SQGKNFEVVFVSGDKDQEAFDAYFAKMPWLAVPFSDSECRAKLNKRFKVRGIPHLVILN 126

Query: 129 G-NGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLASASRNYLV 188
             +G+V + DGV LV   G +AYPFT +R  + L ++E+A K+NQT+ SVL + +R+YL+
Sbjct: 127 ATSGEVYTEDGVELVTVHGTEAYPFTTERINE-LKEQEKAAKDNQTVQSVLGTPTRDYLL 186

Query: 189 SNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIVFVSLEEE 248
           SN G ++P+S+LEGK +GL F + G+     FT  L++ Y  LK+  E FE+V VSL + 
Sbjct: 187 SNKGDRVPISDLEGKYVGLCFVVNGYGPVVQFTSLLAKFYEKLKEVGEKFEVVAVSL-DS 246

Query: 249 DEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNAVDLIKQH 308
           DE+  NE+F  MPWLA+P +D+  +KL  YF++  +P LV+ G DG+TLN N  D+I +H
Sbjct: 247 DEELSNESFAGMPWLAIPQEDKMGEKLARYFELRGLPTLVLIGPDGKTLNNNVADIIDEH 306

Query: 309 GIDA---YPFTPKKHDVVHGKVEA 329
           G DA   +PFT +K +++  K +A
Sbjct: 307 GQDAWEGFPFTAEKMEILAEKAKA 325

BLAST of Cucsat.G8212.T1 vs. ExPASy Swiss-Prot
Match: Q7XPE8 (Probable nucleoredoxin 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0608600 PE=3 SV=2)

HSP 1 Score: 235.3 bits (599), Expect = 1.4e-60
Identity = 123/302 (40.73%), Postives = 182/302 (60.26%), Query Frame = 0

Query: 21  LIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSEFYKELLANKSKNNDFEIIFI 80
           L+ N G ++ +SS+ GKR+ L+FSA WC PCR FTPKL + Y++ L N  KN   EIIFI
Sbjct: 26  LLSNGGKEIPLSSIEGKRICLFFSAHWCRPCRNFTPKLLQIYRK-LRNTCKN--MEIIFI 85

Query: 81  SSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSSIPYLVVID---GNGKVSSAD 140
           S DRD +SF  YF  MPWLA+PF D   ++KL + F +  IP L+ +     +G     D
Sbjct: 86  SLDRDEISFLDYFKGMPWLALPF-DTGLRQKLCVQFDIEHIPALIPLSTTLSHGFRVEED 145

Query: 141 GVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLASASRNYLVSNDGKQIPVS 200
            V LV+E+GVDAYPF   R+ +L    ++A++    +  +L    R Y++S DG + P+S
Sbjct: 146 AVKLVEEYGVDAYPFGAKRRSELEGM-DDARRQGGNLLQLLGCKEREYVISADGIKTPIS 205

Query: 201 ELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKK-KKENFEIVFVSLEEEDEDFFNEAF 260
           +L GK IGLYF       C AFT +L E Y+ LK  +  NF+++F+S++  +E+ F  + 
Sbjct: 206 DLNGKTIGLYFGAHWCPPCRAFTKQLREAYDELKALRPGNFQVIFISMDRNEEE-FQASL 265

Query: 261 KSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNAVDLIKQHGIDAYPFTP 319
            +MPW A+P+ D   Q+L   F +  IP L+I G DG+    +   +I ++G  A+PFT 
Sbjct: 266 SAMPWFAIPYSDTTVQELSRIFTIKGIPTLLILGPDGKVFKTDGRRIISKYGAMAFPFTE 321

BLAST of Cucsat.G8212.T1 vs. ExPASy Swiss-Prot
Match: Q0JIL1 (Probable nucleoredoxin 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0794400 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.3e-58
Identity = 123/299 (41.14%), Postives = 181/299 (60.54%), Query Frame = 0

Query: 21  LIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSEFYKELLANKSKNNDFEIIFI 80
           LI   G++V+IS L GK +GLYF+A W P C  FTP L+  Y +L   K     FE+IF+
Sbjct: 26  LISPTGNEVQISELEGKIIGLYFAANWYPKCEAFTPALTAAYHQL---KEHGAGFEVIFV 85

Query: 81  SSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSSIPYLVVIDGNGKVSSADGVN 140
           S D +  SF+ +   MPW A+PF D+  +K+L   FQ+  IP LVV+  NG+V   D V 
Sbjct: 86  SCDENRPSFERFHRAMPWPAVPFGDIGCKKRLSERFQVEGIPRLVVLAPNGEVVQPDAVE 145

Query: 141 LVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLASASRNYLVSNDGKQIPVSELE 200
           LV  +G  A+PFT  R  +L A  E+ K  +QT+  + + + ++Y V+   +Q+P+S L 
Sbjct: 146 LVHRYGDRAFPFTSARVAELEAD-EQRKFASQTLEKIFSVSGKDY-VNGSQEQVPISSLV 205

Query: 201 GKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIVFVSLEEEDEDFFNEAFKSMP 260
           GK +GLYFS      C  FT KL+ +Y+NLK K E+FEI+++ +++E ED +  +   MP
Sbjct: 206 GKTVGLYFSAHRCAPCIKFTAKLAAIYSNLKGKAEDFEIIYIPMDKE-EDGYLRSCSDMP 265

Query: 261 WLALPFKD-EKCQKLKLYFDVDDIPALVITGQDGRTLNPNAVDLIKQHGIDAYPFTPKK 319
           WLALP+ D      L  YFDV +IP LV+ G DG+T+     +L+  +   A+PFT ++
Sbjct: 266 WLALPYDDGASSGALARYFDVREIPTLVVVGPDGKTVTREGRNLVNLYFDMAFPFTDEQ 318

BLAST of Cucsat.G8212.T1 vs. NCBI nr
Match: KAE8648980.1 (hypothetical protein Csa_009075 [Cucumis sativus])

HSP 1 Score: 954 bits (2465), Expect = 0.0
Identity = 477/477 (100.00%), Postives = 477/477 (100.00%), Query Frame = 0

Query: 1   MAFDAVHDLTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE 60
           MAFDAVHDLTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE
Sbjct: 1   MAFDAVHDLTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE 60

Query: 61  FYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSS 120
           FYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSS
Sbjct: 61  FYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSS 120

Query: 121 IPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLAS 180
           IPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLAS
Sbjct: 121 IPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLAS 180

Query: 181 ASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIV 240
           ASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIV
Sbjct: 181 ASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIV 240

Query: 241 FVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNA 300
           FVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNA
Sbjct: 241 FVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNA 300

Query: 301 VDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSKTREETKDEKVEVSCGSMEEAKDG 360
           VDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSKTREETKDEKVEVSCGSMEEAKDG
Sbjct: 301 VDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSKTREETKDEKVEVSCGSMEEAKDG 360

Query: 361 KAEVSCGSMEETKDGKAEVSCGSMEETKDGKAEVSCGCDGSKNDDDEKMEETKDKMVEVS 420
           KAEVSCGSMEETKDGKAEVSCGSMEETKDGKAEVSCGCDGSKNDDDEKMEETKDKMVEVS
Sbjct: 361 KAEVSCGSMEETKDGKAEVSCGSMEETKDGKAEVSCGCDGSKNDDDEKMEETKDKMVEVS 420

Query: 421 CGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEETKEEIKEGCGCKG 477
           CGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEETKEEIKEGCGCKG
Sbjct: 421 CGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEETKEEIKEGCGCKG 477

BLAST of Cucsat.G8212.T1 vs. NCBI nr
Match: XP_004141531.3 (probable nucleoredoxin 1-2 [Cucumis sativus])

HSP 1 Score: 937 bits (2422), Expect = 0.0
Identity = 469/469 (100.00%), Postives = 469/469 (100.00%), Query Frame = 0

Query: 9   LTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSEFYKELLAN 68
           LTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSEFYKELLAN
Sbjct: 335 LTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSEFYKELLAN 394

Query: 69  KSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSSIPYLVVID 128
           KSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSSIPYLVVID
Sbjct: 395 KSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSSIPYLVVID 454

Query: 129 GNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLASASRNYLVS 188
           GNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLASASRNYLVS
Sbjct: 455 GNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLASASRNYLVS 514

Query: 189 NDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIVFVSLEEED 248
           NDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIVFVSLEEED
Sbjct: 515 NDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIVFVSLEEED 574

Query: 249 EDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNAVDLIKQHG 308
           EDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNAVDLIKQHG
Sbjct: 575 EDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNAVDLIKQHG 634

Query: 309 IDAYPFTPKKHDVVHGKVEASCCGCDGSKTREETKDEKVEVSCGSMEEAKDGKAEVSCGS 368
           IDAYPFTPKKHDVVHGKVEASCCGCDGSKTREETKDEKVEVSCGSMEEAKDGKAEVSCGS
Sbjct: 635 IDAYPFTPKKHDVVHGKVEASCCGCDGSKTREETKDEKVEVSCGSMEEAKDGKAEVSCGS 694

Query: 369 MEETKDGKAEVSCGSMEETKDGKAEVSCGCDGSKNDDDEKMEETKDKMVEVSCGCDGSSK 428
           MEETKDGKAEVSCGSMEETKDGKAEVSCGCDGSKNDDDEKMEETKDKMVEVSCGCDGSSK
Sbjct: 695 MEETKDGKAEVSCGSMEETKDGKAEVSCGCDGSKNDDDEKMEETKDKMVEVSCGCDGSSK 754

Query: 429 NDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEETKEEIKEGCGCKG 477
           NDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEETKEEIKEGCGCKG
Sbjct: 755 NDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEETKEEIKEGCGCKG 803

BLAST of Cucsat.G8212.T1 vs. NCBI nr
Match: TYK00515.1 (protein disulfide isomerase (PDI)-like protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 810 bits (2092), Expect = 2.48e-293
Identity = 425/493 (86.21%), Postives = 438/493 (88.84%), Query Frame = 0

Query: 1   MAFDAVHDLTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE 60
           MAFDAVHD++SLLSS+RRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE
Sbjct: 1   MAFDAVHDISSLLSSERRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE 60

Query: 61  FYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSS 120
           FYKELLAN SKNNDFEIIFISSDRDALSFKAYFSKMPWL+IPFDDLETQKKLKILFQL S
Sbjct: 61  FYKELLANNSKNNDFEIIFISSDRDALSFKAYFSKMPWLSIPFDDLETQKKLKILFQLRS 120

Query: 121 IPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLAS 180
           IPYLVVIDGNGKVSS DGVNLVKEFGVDAYPFT DRKKQLLAQKEEAKKN QTI SVLAS
Sbjct: 121 IPYLVVIDGNGKVSSDDGVNLVKEFGVDAYPFTADRKKQLLAQKEEAKKNPQTIASVLAS 180

Query: 181 ASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIV 240
            SRNYLVSNDGKQIPVS+LEGKLIGLYFSLPGHEHC+ FTPKLSEVYN LKKK ENFEIV
Sbjct: 181 PSRNYLVSNDGKQIPVSKLEGKLIGLYFSLPGHEHCEDFTPKLSEVYNKLKKKDENFEIV 240

Query: 241 FVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNA 300
           FVSLEEEDED F+EAF+SMPWLALPFKDEKCQKLKLYFDV +IPALVITGQDGRTLNPNA
Sbjct: 241 FVSLEEEDEDLFDEAFESMPWLALPFKDEKCQKLKLYFDVGEIPALVITGQDGRTLNPNA 300

Query: 301 VDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSK-----TREETKDEKVEVSCGSME 360
           VDLIKQHGIDAYPFTPKKHDV+HGKVEASC GCDGSK     T EETKDEKVE SCG   
Sbjct: 301 VDLIKQHGIDAYPFTPKKHDVLHGKVEASC-GCDGSKNDNDKTMEETKDEKVEDSCGCDG 360

Query: 361 EAKDGKAEVSCGSMEETKDGKAEVSCG-----------SMEETKDGKAEVSCGCDGSKND 420
             K    +     MEETKDG  EVSCG           +MEETKD K EVSCG DGSKND
Sbjct: 361 SLKKDDDKT----MEETKDGNVEVSCGCDGSSKRDDDKTMEETKDRKVEVSCGYDGSKND 420

Query: 421 DDEKMEETKDKMVEVSCGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEE 477
            DEKMEETKDK VEVSCGCDGS KND+DEKMEET KDGKVEVSCCGCDESKND DQKMEE
Sbjct: 421 GDEKMEETKDKTVEVSCGCDGS-KNDEDEKMEET-KDGKVEVSCCGCDESKNDDDQKMEE 480

BLAST of Cucsat.G8212.T1 vs. NCBI nr
Match: AGH33853.1 (protein disulfide isomerase (PDI)-like protein 4 [Cucumis melo])

HSP 1 Score: 810 bits (2091), Expect = 3.66e-293
Identity = 427/494 (86.44%), Postives = 440/494 (89.07%), Query Frame = 0

Query: 1   MAFDAVHDLTSLLSSDRRDFLIRNNGDQV-KISSLIGKRVGLYFSAGWCPPCRLFTPKLS 60
           MAFDAVHD++SLLSS+RRDFLIRNNGDQV KISSLIGKRVGLYFSAGWCPPCRLFTPKLS
Sbjct: 1   MAFDAVHDISSLLSSERRDFLIRNNGDQVVKISSLIGKRVGLYFSAGWCPPCRLFTPKLS 60

Query: 61  EFYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLS 120
           EFYKELLAN SKNNDFEIIFISSDRDALSFKAYFSKMPWL+IPFDDLETQKKLKILFQL 
Sbjct: 61  EFYKELLANNSKNNDFEIIFISSDRDALSFKAYFSKMPWLSIPFDDLETQKKLKILFQLR 120

Query: 121 SIPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLA 180
           SIPYLVVIDGNGKVSS DGVNLVKEFGVDAYPFT DRKKQLLAQKEEAKKN QTITSVLA
Sbjct: 121 SIPYLVVIDGNGKVSSDDGVNLVKEFGVDAYPFTADRKKQLLAQKEEAKKNTQTITSVLA 180

Query: 181 SASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEI 240
           SASRNYLVSNDGKQIPVS+LEGKLIGLYFSLPGHEHC+ FTPKLSEVYN LKKK ENFEI
Sbjct: 181 SASRNYLVSNDGKQIPVSKLEGKLIGLYFSLPGHEHCEDFTPKLSEVYNKLKKKDENFEI 240

Query: 241 VFVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPN 300
           VFVSLEEEDED F+EAF+SMPWLALPFKDEKCQKLKLYFDV +IPALVITGQDGRTLNPN
Sbjct: 241 VFVSLEEEDEDLFDEAFESMPWLALPFKDEKCQKLKLYFDVGEIPALVITGQDGRTLNPN 300

Query: 301 AVDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSK-----TREETKDEKVEVSCGSM 360
           AVDLIKQHGIDAYPFTPKKHDV+HGKVEASC GCDGSK     T EETKDEKVE SCG  
Sbjct: 301 AVDLIKQHGIDAYPFTPKKHDVLHGKVEASC-GCDGSKNDDDKTMEETKDEKVEDSCGCD 360

Query: 361 EEAKDGKAEVSCGSMEETKDGKAEVSCG-----------SMEETKDGKAEVSCGCDGSKN 420
             +K    +     MEETKDG  EVSCG           +MEETKD K EVSCG DGSKN
Sbjct: 361 GSSKKDDDKT----MEETKDGNVEVSCGCDGSSKRDDDKTMEETKDRKVEVSCGYDGSKN 420

Query: 421 DDDEKMEETKDKMVEVSCGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKME 477
           D DEKMEETKDK VEVSCGCDGS KNDDDEKMEET KDGKVEVSCCGCDESKND DQKM 
Sbjct: 421 DGDEKMEETKDKTVEVSCGCDGS-KNDDDEKMEET-KDGKVEVSCCGCDESKNDDDQKMV 480

BLAST of Cucsat.G8212.T1 vs. NCBI nr
Match: AAU04768.1 (protein disulfide isomerase (PDI)-like protein 4 [Cucumis melo])

HSP 1 Score: 808 bits (2088), Expect = 1.01e-292
Identity = 424/493 (86.00%), Postives = 438/493 (88.84%), Query Frame = 0

Query: 1   MAFDAVHDLTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE 60
           MAFDAVHD++SLLSS+RRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE
Sbjct: 1   MAFDAVHDISSLLSSERRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE 60

Query: 61  FYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSS 120
           FYKELLAN SKNNDFEIIFISSDRDALSFKAYFSKMPWL+IPFDDLETQKKLKILFQL S
Sbjct: 61  FYKELLANNSKNNDFEIIFISSDRDALSFKAYFSKMPWLSIPFDDLETQKKLKILFQLKS 120

Query: 121 IPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLAS 180
           IPYLV IDGNGK SS DGVNLVKEFG DAYPFT DRKKQLLAQKEEAKKN QTITSVLAS
Sbjct: 121 IPYLVGIDGNGKSSSDDGVNLVKEFGSDAYPFTADRKKQLLAQKEEAKKNTQTITSVLAS 180

Query: 181 ASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIV 240
           ASRNYLVSNDGKQIPVS+LEGKLIGLYFSLPGHEHC+ FTPKLSEVYN LKKK ENFEIV
Sbjct: 181 ASRNYLVSNDGKQIPVSKLEGKLIGLYFSLPGHEHCEDFTPKLSEVYNKLKKKDENFEIV 240

Query: 241 FVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNA 300
           FVSLEEEDED F+EAF+SMPWLALPFKDEKCQKLKLYFDV +IPALVITGQDGRTLNPNA
Sbjct: 241 FVSLEEEDEDLFDEAFESMPWLALPFKDEKCQKLKLYFDVGEIPALVITGQDGRTLNPNA 300

Query: 301 VDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSK-----TREETKDEKVEVSCGSME 360
           VDLIKQHGIDAYPFTPKKHDV+HGKVEASC GCDGSK     T EETKDEKVE SCG   
Sbjct: 301 VDLIKQHGIDAYPFTPKKHDVLHGKVEASC-GCDGSKNDDDKTMEETKDEKVEDSCGCDG 360

Query: 361 EAKDGKAEVSCGSMEETKDGKAEVSCG-----------SMEETKDGKAEVSCGCDGSKND 420
            +K    +     MEETKDG  EVSCG           +MEETKD K EVSCG DGSKND
Sbjct: 361 SSKKDDDKT----MEETKDGNVEVSCGCDGSSKRDDDKTMEETKDRKVEVSCGYDGSKND 420

Query: 421 DDEKMEETKDKMVEVSCGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEE 477
            DEKMEETKDK VEVSCGCDGS KND+DEKMEET KDGKVEVSCCGCDESKND DQKMEE
Sbjct: 421 GDEKMEETKDKTVEVSCGCDGS-KNDEDEKMEET-KDGKVEVSCCGCDESKNDDDQKMEE 480

BLAST of Cucsat.G8212.T1 vs. ExPASy TrEMBL
Match: A0A0A0KW15 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G647490 PE=4 SV=1)

HSP 1 Score: 917 bits (2371), Expect = 0.0
Identity = 462/477 (96.86%), Postives = 462/477 (96.86%), Query Frame = 0

Query: 1   MAFDAVHDLTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE 60
           MAFDAVHDLTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE
Sbjct: 1   MAFDAVHDLTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE 60

Query: 61  FYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSS 120
           FYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSS
Sbjct: 61  FYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSS 120

Query: 121 IPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLAS 180
           IPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLAS
Sbjct: 121 IPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLAS 180

Query: 181 ASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIV 240
           ASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIV
Sbjct: 181 ASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIV 240

Query: 241 FVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNA 300
           FVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNA
Sbjct: 241 FVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNA 300

Query: 301 VDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSKTREETKDEKVEVSCGSMEEAKDG 360
           VDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSKTREETKDEKVEVSCGSMEEAKDG
Sbjct: 301 VDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSKTREETKDEKVEVSCGSMEEAKDG 360

Query: 361 KAEVSCGSMEETKDGKAEVSCGSMEETKDGKAEVSCGCDGSKNDDDEKMEETKDKMVEVS 420
           KAE               VSCGSMEETKDGKAEVSCGCDGSKNDDDEKMEETKDKMVEVS
Sbjct: 361 KAE---------------VSCGSMEETKDGKAEVSCGCDGSKNDDDEKMEETKDKMVEVS 420

Query: 421 CGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEETKEEIKEGCGCKG 477
           CGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEETKEEIKEGCGCKG
Sbjct: 421 CGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEETKEEIKEGCGCKG 462

BLAST of Cucsat.G8212.T1 vs. ExPASy TrEMBL
Match: A0A5D3BN43 (Protein disulfide isomerase (PDI)-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001250 PE=4 SV=1)

HSP 1 Score: 810 bits (2092), Expect = 1.20e-293
Identity = 425/493 (86.21%), Postives = 438/493 (88.84%), Query Frame = 0

Query: 1   MAFDAVHDLTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE 60
           MAFDAVHD++SLLSS+RRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE
Sbjct: 1   MAFDAVHDISSLLSSERRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE 60

Query: 61  FYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSS 120
           FYKELLAN SKNNDFEIIFISSDRDALSFKAYFSKMPWL+IPFDDLETQKKLKILFQL S
Sbjct: 61  FYKELLANNSKNNDFEIIFISSDRDALSFKAYFSKMPWLSIPFDDLETQKKLKILFQLRS 120

Query: 121 IPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLAS 180
           IPYLVVIDGNGKVSS DGVNLVKEFGVDAYPFT DRKKQLLAQKEEAKKN QTI SVLAS
Sbjct: 121 IPYLVVIDGNGKVSSDDGVNLVKEFGVDAYPFTADRKKQLLAQKEEAKKNPQTIASVLAS 180

Query: 181 ASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIV 240
            SRNYLVSNDGKQIPVS+LEGKLIGLYFSLPGHEHC+ FTPKLSEVYN LKKK ENFEIV
Sbjct: 181 PSRNYLVSNDGKQIPVSKLEGKLIGLYFSLPGHEHCEDFTPKLSEVYNKLKKKDENFEIV 240

Query: 241 FVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNA 300
           FVSLEEEDED F+EAF+SMPWLALPFKDEKCQKLKLYFDV +IPALVITGQDGRTLNPNA
Sbjct: 241 FVSLEEEDEDLFDEAFESMPWLALPFKDEKCQKLKLYFDVGEIPALVITGQDGRTLNPNA 300

Query: 301 VDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSK-----TREETKDEKVEVSCGSME 360
           VDLIKQHGIDAYPFTPKKHDV+HGKVEASC GCDGSK     T EETKDEKVE SCG   
Sbjct: 301 VDLIKQHGIDAYPFTPKKHDVLHGKVEASC-GCDGSKNDNDKTMEETKDEKVEDSCGCDG 360

Query: 361 EAKDGKAEVSCGSMEETKDGKAEVSCG-----------SMEETKDGKAEVSCGCDGSKND 420
             K    +     MEETKDG  EVSCG           +MEETKD K EVSCG DGSKND
Sbjct: 361 SLKKDDDKT----MEETKDGNVEVSCGCDGSSKRDDDKTMEETKDRKVEVSCGYDGSKND 420

Query: 421 DDEKMEETKDKMVEVSCGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEE 477
            DEKMEETKDK VEVSCGCDGS KND+DEKMEET KDGKVEVSCCGCDESKND DQKMEE
Sbjct: 421 GDEKMEETKDKTVEVSCGCDGS-KNDEDEKMEET-KDGKVEVSCCGCDESKNDDDQKMEE 480

BLAST of Cucsat.G8212.T1 vs. ExPASy TrEMBL
Match: M4QUW9 (Protein disulfide isomerase (PDI)-like protein 4 OS=Cucumis melo OX=3656 GN=PDI4 PE=4 SV=1)

HSP 1 Score: 810 bits (2091), Expect = 1.77e-293
Identity = 427/494 (86.44%), Postives = 440/494 (89.07%), Query Frame = 0

Query: 1   MAFDAVHDLTSLLSSDRRDFLIRNNGDQV-KISSLIGKRVGLYFSAGWCPPCRLFTPKLS 60
           MAFDAVHD++SLLSS+RRDFLIRNNGDQV KISSLIGKRVGLYFSAGWCPPCRLFTPKLS
Sbjct: 1   MAFDAVHDISSLLSSERRDFLIRNNGDQVVKISSLIGKRVGLYFSAGWCPPCRLFTPKLS 60

Query: 61  EFYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLS 120
           EFYKELLAN SKNNDFEIIFISSDRDALSFKAYFSKMPWL+IPFDDLETQKKLKILFQL 
Sbjct: 61  EFYKELLANNSKNNDFEIIFISSDRDALSFKAYFSKMPWLSIPFDDLETQKKLKILFQLR 120

Query: 121 SIPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLA 180
           SIPYLVVIDGNGKVSS DGVNLVKEFGVDAYPFT DRKKQLLAQKEEAKKN QTITSVLA
Sbjct: 121 SIPYLVVIDGNGKVSSDDGVNLVKEFGVDAYPFTADRKKQLLAQKEEAKKNTQTITSVLA 180

Query: 181 SASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEI 240
           SASRNYLVSNDGKQIPVS+LEGKLIGLYFSLPGHEHC+ FTPKLSEVYN LKKK ENFEI
Sbjct: 181 SASRNYLVSNDGKQIPVSKLEGKLIGLYFSLPGHEHCEDFTPKLSEVYNKLKKKDENFEI 240

Query: 241 VFVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPN 300
           VFVSLEEEDED F+EAF+SMPWLALPFKDEKCQKLKLYFDV +IPALVITGQDGRTLNPN
Sbjct: 241 VFVSLEEEDEDLFDEAFESMPWLALPFKDEKCQKLKLYFDVGEIPALVITGQDGRTLNPN 300

Query: 301 AVDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSK-----TREETKDEKVEVSCGSM 360
           AVDLIKQHGIDAYPFTPKKHDV+HGKVEASC GCDGSK     T EETKDEKVE SCG  
Sbjct: 301 AVDLIKQHGIDAYPFTPKKHDVLHGKVEASC-GCDGSKNDDDKTMEETKDEKVEDSCGCD 360

Query: 361 EEAKDGKAEVSCGSMEETKDGKAEVSCG-----------SMEETKDGKAEVSCGCDGSKN 420
             +K    +     MEETKDG  EVSCG           +MEETKD K EVSCG DGSKN
Sbjct: 361 GSSKKDDDKT----MEETKDGNVEVSCGCDGSSKRDDDKTMEETKDRKVEVSCGYDGSKN 420

Query: 421 DDDEKMEETKDKMVEVSCGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKME 477
           D DEKMEETKDK VEVSCGCDGS KNDDDEKMEET KDGKVEVSCCGCDESKND DQKM 
Sbjct: 421 DGDEKMEETKDKTVEVSCGCDGS-KNDDDEKMEET-KDGKVEVSCCGCDESKNDDDQKMV 480

BLAST of Cucsat.G8212.T1 vs. ExPASy TrEMBL
Match: Q5DMW8 (Protein disulfide isomerase (PDI)-like protein 4 OS=Cucumis melo OX=3656 GN=PDI4 PE=4 SV=1)

HSP 1 Score: 808 bits (2088), Expect = 4.89e-293
Identity = 424/493 (86.00%), Postives = 438/493 (88.84%), Query Frame = 0

Query: 1   MAFDAVHDLTSLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE 60
           MAFDAVHD++SLLSS+RRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE
Sbjct: 1   MAFDAVHDISSLLSSERRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSE 60

Query: 61  FYKELLANKSKNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSS 120
           FYKELLAN SKNNDFEIIFISSDRDALSFKAYFSKMPWL+IPFDDLETQKKLKILFQL S
Sbjct: 61  FYKELLANNSKNNDFEIIFISSDRDALSFKAYFSKMPWLSIPFDDLETQKKLKILFQLKS 120

Query: 121 IPYLVVIDGNGKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLAS 180
           IPYLV IDGNGK SS DGVNLVKEFG DAYPFT DRKKQLLAQKEEAKKN QTITSVLAS
Sbjct: 121 IPYLVGIDGNGKSSSDDGVNLVKEFGSDAYPFTADRKKQLLAQKEEAKKNTQTITSVLAS 180

Query: 181 ASRNYLVSNDGKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIV 240
           ASRNYLVSNDGKQIPVS+LEGKLIGLYFSLPGHEHC+ FTPKLSEVYN LKKK ENFEIV
Sbjct: 181 ASRNYLVSNDGKQIPVSKLEGKLIGLYFSLPGHEHCEDFTPKLSEVYNKLKKKDENFEIV 240

Query: 241 FVSLEEEDEDFFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNA 300
           FVSLEEEDED F+EAF+SMPWLALPFKDEKCQKLKLYFDV +IPALVITGQDGRTLNPNA
Sbjct: 241 FVSLEEEDEDLFDEAFESMPWLALPFKDEKCQKLKLYFDVGEIPALVITGQDGRTLNPNA 300

Query: 301 VDLIKQHGIDAYPFTPKKHDVVHGKVEASCCGCDGSK-----TREETKDEKVEVSCGSME 360
           VDLIKQHGIDAYPFTPKKHDV+HGKVEASC GCDGSK     T EETKDEKVE SCG   
Sbjct: 301 VDLIKQHGIDAYPFTPKKHDVLHGKVEASC-GCDGSKNDDDKTMEETKDEKVEDSCGCDG 360

Query: 361 EAKDGKAEVSCGSMEETKDGKAEVSCG-----------SMEETKDGKAEVSCGCDGSKND 420
            +K    +     MEETKDG  EVSCG           +MEETKD K EVSCG DGSKND
Sbjct: 361 SSKKDDDKT----MEETKDGNVEVSCGCDGSSKRDDDKTMEETKDRKVEVSCGYDGSKND 420

Query: 421 DDEKMEETKDKMVEVSCGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEE 477
            DEKMEETKDK VEVSCGCDGS KND+DEKMEET KDGKVEVSCCGCDESKND DQKMEE
Sbjct: 421 GDEKMEETKDKTVEVSCGCDGS-KNDEDEKMEET-KDGKVEVSCCGCDESKNDDDQKMEE 480

BLAST of Cucsat.G8212.T1 vs. ExPASy TrEMBL
Match: A0A5A7T778 (Putative nucleoredoxin 1-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001300 PE=4 SV=1)

HSP 1 Score: 792 bits (2046), Expect = 1.79e-281
Identity = 417/483 (86.34%), Postives = 428/483 (88.61%), Query Frame = 0

Query: 11  SLLSSDRRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSEFYKELLANKS 70
           SLLSS+RRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSEFYKELLAN S
Sbjct: 342 SLLSSERRDFLIRNNGDQVKISSLIGKRVGLYFSAGWCPPCRLFTPKLSEFYKELLANNS 401

Query: 71  KNNDFEIIFISSDRDALSFKAYFSKMPWLAIPFDDLETQKKLKILFQLSSIPYLVVIDGN 130
           KNNDFEIIFISSDRDALSFKAYFSKMPWL+IPFDDLETQKKLKILFQL SIPYLVVIDGN
Sbjct: 402 KNNDFEIIFISSDRDALSFKAYFSKMPWLSIPFDDLETQKKLKILFQLRSIPYLVVIDGN 461

Query: 131 GKVSSADGVNLVKEFGVDAYPFTIDRKKQLLAQKEEAKKNNQTITSVLASASRNYLVSND 190
           GKVSS DGVNLVKEFGVDAYPFT DRKKQLLAQKEEAKKN QTI SVLAS SRNYLVSND
Sbjct: 462 GKVSSDDGVNLVKEFGVDAYPFTADRKKQLLAQKEEAKKNPQTIASVLASPSRNYLVSND 521

Query: 191 GKQIPVSELEGKLIGLYFSLPGHEHCDAFTPKLSEVYNNLKKKKENFEIVFVSLEEEDED 250
           GKQIPVS+LEGKLIGLYFSLPGHEHC+ FTPKLSEVYN LKKK ENFEIVFVSLEEEDED
Sbjct: 522 GKQIPVSKLEGKLIGLYFSLPGHEHCEDFTPKLSEVYNKLKKKDENFEIVFVSLEEEDED 581

Query: 251 FFNEAFKSMPWLALPFKDEKCQKLKLYFDVDDIPALVITGQDGRTLNPNAVDLIKQHGID 310
            F+EAF+SMPWLALPFKDEKCQKLKLYFDV +IPALVITGQDGRTLNPNAVDLIKQHGID
Sbjct: 582 LFDEAFESMPWLALPFKDEKCQKLKLYFDVGEIPALVITGQDGRTLNPNAVDLIKQHGID 641

Query: 311 AYPFTPKKHDVVHGKVEASCCGCDGSK-----TREETKDEKVEVSCGSMEEAKDGKAEVS 370
           AYPFTPKKHDV+HGKVEASC GCDGSK     T EETKDEKVE SCG     K    +  
Sbjct: 642 AYPFTPKKHDVLHGKVEASC-GCDGSKNDNDKTMEETKDEKVEDSCGCDGSLKKDDDKT- 701

Query: 371 CGSMEETKDGKAEVSCG-----------SMEETKDGKAEVSCGCDGSKNDDDEKMEETKD 430
              MEETKDG  EVSCG           +MEETKD K EVSCG DGSKND DEKMEETKD
Sbjct: 702 ---MEETKDGNVEVSCGCDGSSKRDDDKTMEETKDRKVEVSCGYDGSKNDGDEKMEETKD 761

Query: 431 KMVEVSCGCDGSSKNDDDEKMEETCKDGKVEVSCCGCDESKNDGDQKMEETKEEIKEGCG 477
           K VEVSCGCDGS KND+DEKMEET KDGKVEVSCCGCDESKND DQKMEETKEEIKEGCG
Sbjct: 762 KTVEVSCGCDGS-KNDEDEKMEET-KDGKVEVSCCGCDESKNDDDQKMEETKEEIKEGCG 817

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O807632.2e-9049.85Probable nucleoredoxin 1 OS=Arabidopsis thaliana OX=3702 GN=At1g60420 PE=1 SV=1[more]
Q7Y0F24.0e-8447.68Probable nucleoredoxin 1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405... [more]
Q7Y0E88.9e-8447.84Probable nucleoredoxin 1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405... [more]
Q7XPE81.4e-6040.73Probable nucleoredoxin 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g060860... [more]
Q0JIL11.3e-5841.14Probable nucleoredoxin 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g079440... [more]
Match NameE-valueIdentityDescription
KAE8648980.10.0100.00hypothetical protein Csa_009075 [Cucumis sativus][more]
XP_004141531.30.0100.00probable nucleoredoxin 1-2 [Cucumis sativus][more]
TYK00515.12.48e-29386.21protein disulfide isomerase (PDI)-like protein 4 [Cucumis melo var. makuwa][more]
AGH33853.13.66e-29386.44protein disulfide isomerase (PDI)-like protein 4 [Cucumis melo][more]
AAU04768.11.01e-29286.00protein disulfide isomerase (PDI)-like protein 4 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A0A0KW150.096.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G647490 PE=4 SV=1[more]
A0A5D3BN431.20e-29386.21Protein disulfide isomerase (PDI)-like protein 4 OS=Cucumis melo var. makuwa OX=... [more]
M4QUW91.77e-29386.44Protein disulfide isomerase (PDI)-like protein 4 OS=Cucumis melo OX=3656 GN=PDI4... [more]
Q5DMW84.89e-29386.00Protein disulfide isomerase (PDI)-like protein 4 OS=Cucumis melo OX=3656 GN=PDI4... [more]
A0A5A7T7781.79e-28186.34Putative nucleoredoxin 1-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 227..247
NoneNo IPR availableCOILSCoilCoilcoord: 157..177
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 177..315
e-value: 3.7E-31
score: 109.8
coord: 13..154
e-value: 7.3E-39
score: 134.8
NoneNo IPR availablePANTHERPTHR13871THIOREDOXINcoord: 5..323
NoneNo IPR availablePANTHERPTHR13871:SF95NUCLEOREDOXIN 1-2-RELATEDcoord: 5..323
NoneNo IPR availableCDDcd03009TryX_like_TryX_NRXcoord: 19..152
e-value: 4.79288E-65
score: 204.056
IPR012336Thioredoxin-like foldPFAMPF13905Thioredoxin_8coord: 201..294
e-value: 2.8E-24
score: 85.2
coord: 36..133
e-value: 6.0E-29
score: 100.2
IPR013766Thioredoxin domainPROSITEPS51352THIOREDOXIN_2coord: 1..162
score: 11.55538
IPR013766Thioredoxin domainPROSITEPS51352THIOREDOXIN_2coord: 175..306
score: 8.987402
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 185..296
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 22..134

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G8212Cucsat.G8212gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G8212.T1.E1Cucsat.G8212.T1.E1exon
Cucsat.G8212.T1.E2Cucsat.G8212.T1.E2exon
Cucsat.G8212.T1.E3Cucsat.G8212.T1.E3exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G8212.T1.C1Cucsat.G8212.T1.C1CDS
Cucsat.G8212.T1.C2Cucsat.G8212.T1.C2CDS
Cucsat.G8212.T1.C3Cucsat.G8212.T1.C3CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G8212.T1Cucsat.G8212.T1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016853 isomerase activity