Cucsat.G8004.T3 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G8004.T3
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	3-isopropylmalate dehydratase large subunit, chloroplastic isoform B
Location	ctg1556: 2243692 .. 2246613 (-)
RNA-Seq Expression	Cucsat.G8004.T3
Synteny	Cucsat.G8004.T3

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAAGGCCCAAAAAGATCTGTCTTCCTCTTCCTCCTCAAGAAAAACGACGAACCTGAAAAACCTCCTCAGCTTCAAGGTTCCCGGAGCTTCATTGCAGAATGGATTCGGCCTCCAAATTCCTTCGTAGTTTAGCTAATGCCACCAACAAAAACACCGTTATCAACGTCTGTTTAGTCGTTTCCTTCGCCGCACTGACCGCAAGGTCAATCAAGCAGGAGAGACAAATTGAAGCTCTGGAAACCGAGAAAAATTCACTTCTCAATTCCAATAAAGCGTTGAAGAAAACCATGTGGGATTGGAAGCAACAGCTATTCGCCGAAGCCTCAACCGAATCTGCTTTAGTCCCTCTTGCCAGGATCAAAGCTATCTATGGCGAAGCTCCCATTTCCCCTTCCGGTAATATACGCCATGCTTCTCTCTGTTCTGTTCTTTATGTTTTTTTACTTTCTGTTGTTGGAAAAGTTAGTGTTTAGTTGAATGTGTCGAAATGTTAGTGATTCGTGTTAGAAATTGAGTGTCTGAAAATGATGGGTCGTTCCAAGCATTTCAAATTCCTTATTCGTACTTTACAAATTGTTGGGTTTCGTTGCTACTTCAACTATATCATCATTGACCTGATTTTTCTTATCATCCATTTCTGAACTTCAATGTTGTTATGATTAAGTACCATGTTCTTGGTTTTCATGGATTCTAATCTCTCAGAACTATTAGCCAGAACCCAGGAAACAAATAAGGTATGAGATTATGAATCTAAAAAGAATAGTTTTCATCGCACCATTATATGAAAAAAAGTGTGTTTGAGAAGTGAATGTTCTATTATGGGATGCTGGAATTGAAGACTAAAGTTAAGATTGATAAGGTCAACAGAACCTCCACCTATCTTCTACCTTGGCTATAAACTCGAACTACCCAAATGGTCTCGTACTAATTAAGATAATTGTTCTCACTCATGTACTAATGATTGTACTCATGTTTGCCCTAAAGATAATCGATAAAGAAGGAACCATACCCAGCCAAGCCTAAAGCCACTGAGCAATTCTCTACCTTCATCCTTTCCTACATTTCAATTTACGAATCACCTTGACAACTTTCACCCTGATAGACCATGCTGATCATTCCTGTTCTCTGCCTATTAAGTTAACGTTCCTGCCCAGCTGATGCTAAGGTTTTCATTATGGGGATAGTATATGCTGTATTAAACTTTACCGGGAAAGCACTGCTATAGTACTACGATTTTATATGAGAAAGAGACGCTTGAAAAGCTTATATGCGAAAGGAAGCAATTTCTCTTATTGGAAGCTTAAGAAAGGCACTCCAATTGAAGTTGATTGTGAAGGCAGAATAGTCATAGATGATAGGAGGAGTTGGAAGGGGAAGCTAAAAGACCATTGAACTTTTCCAATTCTTGAACGTCTTCCCCATATCTCTCCCTATTATTAGAAATCTGTTAATTTCTTTCTTTCTAGCCACCAGCCACTATACCTAATCCTGGACTGCATTTTGCTATAGGATCTCTCTCCTTTCGTAGTTGGTTCAGCACAGAAGCTGCATCTAACTATCCTTGGTCCGTAAATAAAAAACCGAGAAAATGATGATGTAGAGGAAAACTAGTAAAAGGCTTTGTGTCTTCTTTGGATTCCTACATAGTATGCACCAACTTAACTTGGTTGCAAGATCAGCAATTGTCATCTTCAAATTGGATTTTTGTATCTCCATTGTGTTTAGGTCAAGACACACAAACCAACAATATCTCTCGATTTTTATAAATTGGTTACCTTTCCAATTTGATAGTGATAAGTTTCTAGGTAGTGAGGAAACCAGTCTGCTATCTAGCTGTCATTGATCAACAGAAATGCGTTGCTTTCGTCAACCTTATGATAATATTACCCTGTTGACGATGACTCTAGTATGTATTTCGTAACTTTTGGGGAGTTATGAAGATCTTTGCTCAAGAATTGGTACTAAAATGTCTGCTTTTAGTACAAACGGATTGTAATTTCAGATAGATTGGAAGATAAACAGCACGAGTAGATTTTTTGAAAAAAAAGATTCAAATGCATGTTTGATGCAAAACTTTTACCCTTTTTCCTGGCAGAGTTCTTGCATAACAATGTTGGGAATCTATTAAACGATTGCCTATAAATTAATAATCGTAATGTAATGTGAGAGTTTTAAGTCTCGATTCAGATTATGTCAAATCCTATCCATATTATTTCATTCTCCCATGCATATTATTTAGTTATTGAACACATGAATCAAATACTCTTGTGGATAGTATTTTTTCAAGTTTGATTACAAAAAGAAGTGTTTGTTTAGAAAGAGTTTTAAGTGTCTAAAAAGTCATTTTGAATATGTTCAAGTCTATTATTGTACAGGCTAGCTTTTACAGATTTATCCATTGATCCCTTTTTTATGGACCATACAGGAGCAGTAAATGCCACAACCGAAGATGCAACTTCACGAAGCTCCAAACTTATGGTTTAATGTTAGCTTCACTCAAGTTTTCCAGGTTTTGTTTCCTTTGTTCACAAATTGGCTGAAGAATCGAACTCCATTAAGATTTGCATATTTCTATGCTCAATGCTAAAAAAAGTAGTGCAGAATATAAGGAATTAGATTTTTCCTTCTTCAGGCACGTATTATCAACTTCAAAGTTAGATATCATATTCCCTACCCTCACATTTTCAGAAGGAAAAAAGAAAAAAAAATCTTTTAGTTTGCCATTAAATGCTCTAGAATAAAACAGCTGATGTAGTCAAAAGCTCCATGATGACAAAGCATCATTATACAACCTTTTGCAACTACTTTAAAATAGGTTAAAGTGTGGTTTTAGTCTCGCACCTTGAAGATTGTTTAGGTTTAGTTTCTTTTTAATCTTCGCGCTTTTTATAAATTTTAAAGTTAATTTGTTATTGTTGA

Coding sequence (CDS)

ATGGATTCGGCCTCCAAATTCCTTCGTAGTTTAGCTAATGCCACCAACAAAAACACCGTTATCAACGTCTGTTTAGTCGTTTCCTTCGCCGCACTGACCGCAAGGTCAATCAAGCAGGAGAGACAAATTGAAGCTCTGGAAACCGAGAAAAATTCACTTCTCAATTCCAATAAAGCGTTGAAGAAAACCATGTGGGATTGGAAGCAACAGCTATTCGCCGAAGCCTCAACCGAATCTGCTTTAGTCCCTCTTGCCAGGATCAAAGCTATCTATGGCGAAGCTCCCATTTCCCCTTCCGGAGCAGTAAATGCCACAACCGAAGATGCAACTTCACGAAGCTCCAAACTTATGGTTTAA

Protein sequence

MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKALKKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGAVNATTEDATSRSSKLMV

Homology

[Close]

BLAST of Cucsat.G8004.T3 vs. NCBI nr
Match: XP_004151748.1 (uncharacterized protein LOC101219754 [Cucumis sativus] >KGN54811.2 hypothetical protein Csa_012251 [Cucumis sativus])

HSP 1 Score: 221 bits (563), Expect = 2.19e-72
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL
Sbjct: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGAVNATTEDATSRSSKLMV 118
           KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGAVNATTEDATSRSSKLMV
Sbjct: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGAVNATTEDATSRSSKLMV 118

[Close]

BLAST of Cucsat.G8004.T3 vs. NCBI nr
Match: XP_008444149.1 (PREDICTED: uncharacterized protein LOC103487583 [Cucumis melo])

HSP 1 Score: 199 bits (507), Expect = 7.62e-64
Identity = 106/118 (89.83%), Postives = 111/118 (94.07%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD ASKFLR L+N  NKNT+INVCLV SFAAL+ARSIKQERQIEALE EKNSLL+SNKAL
Sbjct: 1   MDLASKFLRILSNDNNKNTLINVCLVFSFAALSARSIKQERQIEALEAEKNSLLDSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGAVNATTEDATSRSSKLMV 118
           KKTMWDWKQQLFAEAST+SALVPLARIKAIYGEAPISPSGAVNA TEDATSRSSKLMV
Sbjct: 61  KKTMWDWKQQLFAEASTQSALVPLARIKAIYGEAPISPSGAVNAATEDATSRSSKLMV 118

[Close]

BLAST of Cucsat.G8004.T3 vs. NCBI nr
Match: XP_038897052.1 (uncharacterized protein LOC120085226 [Benincasa hispida])

HSP 1 Score: 184 bits (467), Expect = 9.55e-58
Identity = 96/118 (81.36%), Postives = 106/118 (89.83%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD ASKFLR++ NATN NT+INVCLV+SF AL+ARSIKQ+R+IEALE EK SLLNSNKAL
Sbjct: 1   MDLASKFLRTVTNATNNNTLINVCLVLSFGALSARSIKQQREIEALEAEKVSLLNSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGAVNATTEDATSRSSKLMV 118
           KKTMWDWKQQLFAEAST+SALVPLARIKAIYGEAPISPSG  +  TEDA S+ SKLMV
Sbjct: 61  KKTMWDWKQQLFAEASTDSALVPLARIKAIYGEAPISPSGVGHVATEDANSQGSKLMV 118

[Close]

BLAST of Cucsat.G8004.T3 vs. NCBI nr
Match: XP_022976887.1 (uncharacterized protein LOC111477117 [Cucurbita maxima])

HSP 1 Score: 182 bits (463), Expect = 3.89e-57
Identity = 93/118 (78.81%), Postives = 106/118 (89.83%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD ASKFLR+L NATN NT+INVCLV+SF AL+ARSIKQ+R+IEALE EK+SLLNSNKAL
Sbjct: 1   MDLASKFLRTLTNATNNNTLINVCLVISFGALSARSIKQQREIEALEAEKDSLLNSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGAVNATTEDATSRSSKLMV 118
           KKTMWDWKQQL++EAST+SAL+PLARIKAIY EAP+SPSGA  A T DA S+ SKLMV
Sbjct: 61  KKTMWDWKQQLYSEASTDSALIPLARIKAIYSEAPVSPSGAEQAATGDANSQGSKLMV 118

[Close]

BLAST of Cucsat.G8004.T3 vs. NCBI nr
Match: XP_022937146.1 (uncharacterized protein LOC111443531 [Cucurbita moschata] >XP_023536544.1 uncharacterized protein LOC111797682 [Cucurbita pepo subsp. pepo] >KAG7024766.1 hypothetical protein SDJN02_13584 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 179 bits (453), Expect = 1.30e-55
Identity = 90/118 (76.27%), Postives = 107/118 (90.68%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD A+KFLR++ +ATN NT+INVCLV+SF AL+ARSIKQ+R+IEALE EK+SLLNSNK+L
Sbjct: 1   MDLATKFLRTVTSATNNNTLINVCLVISFGALSARSIKQQREIEALEAEKDSLLNSNKSL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGAVNATTEDATSRSSKLMV 118
           KKTMWDWKQQL+++AST+SALVPLARIKAIYGEAP+SPSGA  A T DA S+ SKLMV
Sbjct: 61  KKTMWDWKQQLYSDASTDSALVPLARIKAIYGEAPVSPSGAEQAATGDANSQGSKLMV 118

[Close]

BLAST of Cucsat.G8004.T3 vs. ExPASy TrEMBL
Match: A0A1S3B9R7 (uncharacterized protein LOC103487583 OS=Cucumis melo OX=3656 GN=LOC103487583 PE=4 SV=1)

HSP 1 Score: 199 bits (507), Expect = 3.69e-64
Identity = 106/118 (89.83%), Postives = 111/118 (94.07%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD ASKFLR L+N  NKNT+INVCLV SFAAL+ARSIKQERQIEALE EKNSLL+SNKAL
Sbjct: 1   MDLASKFLRILSNDNNKNTLINVCLVFSFAALSARSIKQERQIEALEAEKNSLLDSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGAVNATTEDATSRSSKLMV 118
           KKTMWDWKQQLFAEAST+SALVPLARIKAIYGEAPISPSGAVNA TEDATSRSSKLMV
Sbjct: 61  KKTMWDWKQQLFAEASTQSALVPLARIKAIYGEAPISPSGAVNAATEDATSRSSKLMV 118

[Close]

BLAST of Cucsat.G8004.T3 vs. ExPASy TrEMBL
Match: A0A0A0L380 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G364020 PE=4 SV=1)

HSP 1 Score: 191 bits (486), Expect = 7.12e-61
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL
Sbjct: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSG 100
           KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSG
Sbjct: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSG 100

[Close]

BLAST of Cucsat.G8004.T3 vs. ExPASy TrEMBL
Match: A0A6J1IGY3 (uncharacterized protein LOC111477117 OS=Cucurbita maxima OX=3661 GN=LOC111477117 PE=4 SV=1)

HSP 1 Score: 182 bits (463), Expect = 1.88e-57
Identity = 93/118 (78.81%), Postives = 106/118 (89.83%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD ASKFLR+L NATN NT+INVCLV+SF AL+ARSIKQ+R+IEALE EK+SLLNSNKAL
Sbjct: 1   MDLASKFLRTLTNATNNNTLINVCLVISFGALSARSIKQQREIEALEAEKDSLLNSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGAVNATTEDATSRSSKLMV 118
           KKTMWDWKQQL++EAST+SAL+PLARIKAIY EAP+SPSGA  A T DA S+ SKLMV
Sbjct: 61  KKTMWDWKQQLYSEASTDSALIPLARIKAIYSEAPVSPSGAEQAATGDANSQGSKLMV 118

[Close]

BLAST of Cucsat.G8004.T3 vs. ExPASy TrEMBL
Match: A0A6J1F9J6 (uncharacterized protein LOC111443531 OS=Cucurbita moschata OX=3662 GN=LOC111443531 PE=4 SV=1)

HSP 1 Score: 179 bits (453), Expect = 6.29e-56
Identity = 90/118 (76.27%), Postives = 107/118 (90.68%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD A+KFLR++ +ATN NT+INVCLV+SF AL+ARSIKQ+R+IEALE EK+SLLNSNK+L
Sbjct: 1   MDLATKFLRTVTSATNNNTLINVCLVISFGALSARSIKQQREIEALEAEKDSLLNSNKSL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPISPSGAVNATTEDATSRSSKLMV 118
           KKTMWDWKQQL+++AST+SALVPLARIKAIYGEAP+SPSGA  A T DA S+ SKLMV
Sbjct: 61  KKTMWDWKQQLYSDASTDSALVPLARIKAIYGEAPVSPSGAEQAATGDANSQGSKLMV 118

[Close]

BLAST of Cucsat.G8004.T3 vs. ExPASy TrEMBL
Match: A0A6J1CGB8 (uncharacterized protein LOC111011272 OS=Momordica charantia OX=3673 GN=LOC111011272 PE=4 SV=1)

HSP 1 Score: 172 bits (436), Expect = 2.53e-53
Identity = 91/119 (76.47%), Postives = 105/119 (88.24%), Query Frame = 0


Query: 1   MDSASKFLRSLANATNKNTVINVCLVVSFAALTARSIKQERQIEALETEKNSLLNSNKAL 60
           MD A+KFLR+L NA+N  T+INVCLVVSF AL+ARSIKQ+R+IEALE EK+SLLNSNKAL
Sbjct: 1   MDMATKFLRTLTNASNNKTLINVCLVVSFGALSARSIKQQREIEALEAEKDSLLNSNKAL 60

Query: 61  KKTMWDWKQQLFAEASTESALVPLARIKAIYGEAPIS-PSGAVNATTEDATSRSSKLMV 118
           KK+MWDWKQQLFAEAS+ESALVPLARIKAIYGE PIS P+GA +A TED  S+ SK +V
Sbjct: 61  KKSMWDWKQQLFAEASSESALVPLARIKAIYGEVPISSPTGAGHAATEDENSQGSKFVV 119

The following BLAST results are available for this feature:

BLAST of Cucsat.G8004.T3 vs.


Match Name	E-value	Identity	Description

BLAST of Cucsat.G8004.T3 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004151748.1	2.19e-72	100.00	uncharacterized protein LOC101219754 [Cucumis sativus] >KGN54811.2 hypothetical ...	[more]
XP_008444149.1	7.62e-64	89.83	PREDICTED: uncharacterized protein LOC103487583 [Cucumis melo]	[more]
XP_038897052.1	9.55e-58	81.36	uncharacterized protein LOC120085226 [Benincasa hispida]	[more]
XP_022976887.1	3.89e-57	78.81	uncharacterized protein LOC111477117 [Cucurbita maxima]	[more]
XP_022937146.1	1.30e-55	76.27	uncharacterized protein LOC111443531 [Cucurbita moschata] >XP_023536544.1 unchar...	[more]

BLAST of Cucsat.G8004.T3 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B9R7	3.69e-64	89.83	uncharacterized protein LOC103487583 OS=Cucumis melo OX=3656 GN=LOC103487583 PE=...	[more]
A0A0A0L380	7.12e-61	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G364020 PE=4 SV=1	[more]
A0A6J1IGY3	1.88e-57	78.81	uncharacterized protein LOC111477117 OS=Cucurbita maxima OX=3661 GN=LOC111477117...	[more]
A0A6J1F9J6	6.29e-56	76.27	uncharacterized protein LOC111443531 OS=Cucurbita moschata OX=3662 GN=LOC1114435...	[more]
A0A6J1CGB8	2.53e-53	76.47	uncharacterized protein LOC111011272 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 29..63
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 99..118
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 96..118
None	No IPR available	PANTHER	PTHR38355	OS06G0149500 PROTEIN	coord: 1..118

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G8004	Cucsat.G8004	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8004.T3.E1	Cucsat.G8004.T3.E1	exon
Cucsat.G8004.T3.E2	Cucsat.G8004.T3.E2	exon
Cucsat.G8004.T3.E3	Cucsat.G8004.T3.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8004.T3.C2	Cucsat.G8004.T3.C2	CDS
Cucsat.G8004.T3.C1	Cucsat.G8004.T3.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G8004.T3	Cucsat.G8004.T3-protein	polypeptide